scholarly journals Incidence and Susceptibility Pattern of Clinical Isolates of Pseudomonas aeruginosa from Selected Teaching Hospitals in Southwest Nigeria

2020 ◽  
pp. 110-114
Author(s):  
I. A. Azeez ◽  
S. L. Owolabi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Agents ◽  
Agar Plate ◽  
Teaching Hospitals ◽  
Southwest Nigeria ◽  
Life Threatening ◽  
Oxidase Test ◽  
Male Patients ◽  
And Gender ◽  
Age Range

Despite advances in sanitation facilities and the introduction of various antimicrobial agents with anti-pseudomonal activities, the life-threatening infections caused by Pseudomonas aeruginosa has continue to be hospital infections. This study was aimed to determining the incidence and susceptibility patterns of P. aeruginosa from some teaching hospitals in Southwest, Nigeria. Seventy-seven (77) isolates of Pseudomonas species were obtained from different clinical specimens from three (3) teaching hospitals in southwest, Nigeria. The isolates were re-identified by culturing on cetrimide agar plate and oxidase test was performed on the isolates. Information on the site of isolation, age and gender of the patient were obtained from request forms. Pseudomonas aeruginosa infections is mostly associated with the age range of 30-39 years in male patients and 10-19 years in female patients (P<0.05). The wound swab (29.87%) has the highest rate of P. aeruginosa infections closely followed by ear swab (22.08%). There was a statistical significant increase in the mean diameter of zone of inhibition of ciprofloxacin against P. aeruginosa when compared with other antibacterial agents (F-ratio = 18.798, P< 0.0001). However, P. aeruginosa was absolutely resistant to ceftazidime and augmentin.

scholarly journals SNAKE BITE

2010 ◽  
Vol 17 (02) ◽  
pp. 263-268
Author(s):  
KAMRAN ZAHEER BUTT ◽  
FAHEEM ANWAR ◽  
MOHAMMAD RIZWAN
Keyword(s):  
First Aid ◽  
Snake Bite ◽  
Clinical Effects ◽  
Field Hospital ◽  
Laboratory Findings ◽  
Life Threatening ◽  
History Of ◽  
Male Patients ◽  
Age Range ◽  
Study Setting

Introduction: 15 percent of the snakes are poisonous and present a potential life threatening risk to human lives. Objectives: (1) To review the demographic, epidemiological, clinical and laboratory findings of 48 patients of snake bite treated in a field hospital. (2) To evaluate the treatment and outcome of these patients. Design: A retrospective study. Setting: Field hospital in rural Sindh. Period: From January 2002 to December 2003. Material & Methods: All patients diagnosed with snake bite had first aid treatment by a either a nursing staff close to the place of bite or by a quack and later on transferred to field hospital. The first aid treatment consisted of pain relief (injectable diclofenac, oral acetaminophen), application of bandage or tourniquet proximal to the bite, antihistamine (oral or injectable chlorpheniramine) anti- inflammatory (injection hydrocortisone) and immobilization of the affected limb with a splint. Results: We are treated 48 patients with snake bite. There were 45(94%) male patients and 3(6%) female patients. Age range was 18 to 56 years with a mean age of 29.8 years. 35(73%) patients suffered from snake bite between the months of May and September. The timing of the bite was also peculiar with 36(75%) patients bitten between 8pm and 8am whereas only 12(25%) patients during other times of the day. 38(79%) patients gave history of seeing the snake themselves and 10(21%) patients were not able to see the snake mainly because of darkness. Conclusion It should be remembered that not all snakes are poisonous and that they are more afraid of humans than we are of them. Psychological effects of the bite are at times more devastating than the clinical effects, therefore patient reassurance forms part of the treatment.

An Evaluation of Success Rate of Endoscopic Endonasal Dacryocystorhinostomy at Tertiary Care Hospital

2021 ◽  
Vol 15 (12) ◽  
pp. 3413-3414
Author(s):  
M Wahid Saleem ◽  
Lubna Wajid ◽  
Shahid Ali
Keyword(s):  
Success Rate ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Care Hospital ◽  
Endoscopic Endonasal ◽  
Surgical Success ◽  
Female Patients ◽  
Male Patients ◽  
And Gender ◽  
Age Range

Objective: To evaluate the success rate of endoscopic endonasal dacryocystorhinostomy at tertiary care hospital. Material and methods: Between July 2020 to December 2020, total 50 patients having age between 20-60 years, both gender, cases of epiphora, cases with evidence of obstruction were recruited from Department of ENT, DG Khan Medical Collge, DG Khan. Success of DCR was evaluated. Results: Fifty cases were selected for this study. Age range was 20-40 years with mean age 38.80 ± 8.74 years. DCR was successful in 47 (94%) patients while in 3 (6%) patients surgery was not successful. Male patients were 35 (70%) while female patients were 15 (30%). Surgical success was noted in 33 (94.29%) male patients and 14 (93.33%) female patients. Insignificant (P = 1.00) association between surgical success and gender was noted. Conclusion: In this study high success rate of DCR was noted. Males were prominent. Most of the patients were between 20-40 years and no association of success of surgery with age group and gender found. Keywords: Dacryocystorhinostomy, Epiphora, Intubation, Chronic dacryocystitis

scholarly journals Clinical characteristics and correlates of patients with heart failure with mid-range ejection fraction in southwest Nigeria

2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Adeseye Akintunde
Keyword(s):  
Heart Failure ◽  
Ejection Fraction ◽  
Clinical Characteristics ◽  
Left Ventricular ◽  
Teaching Hospitals ◽  
High Systolic Blood Pressure ◽  
Reduced Ejection Fraction ◽  
New Class ◽  
Southwest Nigeria ◽  
And Gender

A new class of Heart Failure (HF) phenotypes, HF with midrange Ejection Fraction (HFmrEF), was recently introduced, but its clinical characteristics and therapeutic distinctiveness are not yet well understood. This study aimed to describe the clinical characteristics, echocardiographic features, and other correlates of HFmrEF in southwest Nigeria. Two hundred and sixty-nine consecutive HF subjects who had echocardiography done in the cardiology clinics of two teaching hospitals were recruited for this study. Clinical parameters such as age, body mass index, waist-hip ratio, and gender were determined. The presence of comorbidities, such as hypertension and diabetes, was also assessed. Statistical analysis was done, and p <0.05 was taken as statistically significant. HFmrEF subjects constituted 27.5% of total cohort, while subjects with HF with preserved Ejection Fraction (HFpEF) and HF with reduced Ejection Fraction (HFrEF) were 29.0% and 43.5% respectively. HFmrEF was more likely to be associated with high systolic blood pressure and obesity. The clinical characteristics of HFmrEF were intermediate between those of the other two HF phenotypes. Prevalence of comorbidities, such as anaemia, iron deficiency, pulmonary hypertension, and left ventricular hypertrophy were also intermediate between HFpEF and HFrEF. Atrial fibrillation was commonest among HFmrEF subjects. There was no significant age or gender variation between the three phenotypes. Patients with HFmrEF have clinical and demographic distinctiveness that are often intermediate between HFpEF and HFrEF phenotypes. Further studies of this HF phenotype will help in understanding its therapeutic identity and its prognosis among Africans.

scholarly journals DIABETIC RETINOPATHY

2010 ◽  
Vol 17 (02) ◽  
pp. 257-262
Author(s):  
NAUMAN AYUB SHAIKH ◽  
FAROOQ AHMAD
Keyword(s):  
First Aid ◽  
Snake Bite ◽  
Clinical Effects ◽  
Field Hospital ◽  
Laboratory Findings ◽  
Life Threatening ◽  
History Of ◽  
Male Patients ◽  
Age Range ◽  
Study Setting

Introduction: 15 percent of the snakes are poisonous and present a potential life threatening risk to human lives. Objectives: (1) To review the demographic, epidemiological, clinical and laboratory findings of 48 patients of snake bite treated in a field hospital. (2) To evaluate the treatment and outcome of these patients. Design: A retrospective study. Setting: Field hospital in rural Sindh. Period: From January 2002 to December 2003. Material & Methods: All patients diagnosed with snake bite had first aid treatment by a either a nursing staff close to the place of bite or by a quack and later on transferred to field hospital. The first aid treatment consisted of pain relief (injectable diclofenac, oral acetaminophen), application of bandage or tourniquet proximal to the bite, antihistamine (oral or injectable chlorpheniramine) anti- inflammatory (injection hydrocortisone) and immobilization of the affected limb with a splint. Results: We are treated 48 patients with snake bite. There were 45(94%) male patients and 3(6%) female patients. Age range was 18 to 56 years with a mean age of 29.8 years. 35(73%) patients suffered from snake bite between the months of May and September. The timing of the bite was also peculiar with 36(75%) patients bitten between 8pm and 8am whereas only 12(25%) patients during other times of the day. 38(79%) patients gave history of seeing the snake themselves and 10(21%) patients were not able to see the snake mainly because of darkness. Conclusion It should be remembered that not all snakes are poisonous and that they are more afraid of humans than we are of them. Psychological effects of the bite are at times more devastating than the clinical effects, therefore patient reassurance forms part of the treatment. 

scholarly journals Ocurrence of Porphyromonas gingivalis in patients with periodontitis in Brazil

2010 ◽  
Vol 9 (3) ◽  
Author(s):  
Karla Zanini Kantorski ◽  
Ariana Soares Rodrigues ◽  
Gláucia Santos Zimmemann ◽  
Roberto Fraga Moreira Lotufo
Keyword(s):  
Porphyromonas Gingivalis ◽  
Clinical Laboratory ◽  
Chi Square ◽  
Gender And Age ◽  
Significant Difference ◽  
Chi Square Test ◽  
Oral Microbiology ◽  
Male Patients ◽  
And Gender ◽  
Age Range

 The aim of this study was to evaluate the occurrence of Porphyromonas gingivalis in patients with periodontitis in Brazil and investigate whether there is a relationship between your occurrence and gender and age. Analysis was performed of 1,386 results of microbiological examinations of samples of subgingival microbiota in patients with periodontitis. Collection was performed by periodontists in private practice and processed by culture in clinical laboratory of oral microbiology. The chi-square test was used to verify the association of occurrence of P. gingivalis with gender and age. It was observed that 59% of examinations were of female patients and 41% were of male patients, most of which aged more than 40 years (64.3%). The occurrence of P. gingivalis was 17.8%. There was no significant difference in the presence of this bacterium in the different age ranges, yet there was a significantly higher occurrence in males. It was observed that, regardless of the age range, the occurrence of P. gingivalis was always significantly higher in males. It was concluded that the occurrence of P. gingivalis in patients with periodontitis in Brazil was 17.8% and was associated with gender, yet had no association with the age range.

scholarly journals Congenita ptosis; Frequency of congenital ptosis.

2020 ◽  
Vol 27 (08) ◽  
pp. 1602-1605
Author(s):  
Sikander Hayat Khan Niazi
Keyword(s):  
Age Groups ◽  
Age Group ◽  
Age And Gender ◽  
Congenital Ptosis ◽  
Lacrimal Apparatus ◽  
Total Percentage ◽  
Gender And Age ◽  
Male Patients ◽  
And Gender ◽  
Age Range

Objectives: To compare the frequency of congenital ptosis of Pakistani children with the children of the world. Study Design: Comparative study. Setting: Al Shifa Tertiary Eye Hospital Rawalpindi. Period: August 2013 to September 2016. Material & Methods: The record of admitted patients of all malformations of eyes was collected from pediatric eye department from age zero to 15 years. The data of each eye malformation was documented separately as per age and gender. The total numbers of eye malformations were arranged according to prevalence, age and gender. The data was analyzed separately for frequency, age and gender. All data was analyzed and arranged according to percentage of frequency, gender and age. The age data was analyzed making different age groups. Seven groups of age were made with 2 years difference between each group starting from age 0 to 15 years. The number of male and females were calculated in each age group. Total numbers of each malformation was calculated separately and percentage of each malformation calculated as per simple mathematical way to total malformations. Results: The data of five hundreds and fourteen congenital malformations of eyes was collected. The numbers of male patients were 289 and female patients were 225. As per percentage the males were 56.2% and females patients were 43.8 %. The patient age range was from age zero to fifteen years. The majority of the patients were between ages zero to two years. The total numbers of patients in this age group were 222. Out of 222 patients the males were 120 and females were 102. The total percentage of this age group was 43.2 % among all age groups. The most common frequency of eye malformation was cataract i.e. 116 (22.6 %) patients followed by lacrimal apparatus anomalies i.e. 103(20 %) and ptosis was 45 (8.8 %). Conclusion: The frequency of congenital ptosis is the third most common eye malformation among total eye malformations in this study.

Aktivitas Antibakteri Ekstrak Air Kopi Robusta (Coffea Canephora) terhadap Bakteri Pseudomonas aeruginosa

2021 ◽  
Vol 3 (1) ◽  
pp. 38-40
Author(s):  
Naura Mufida Marsya ◽  
Hendro Sudjono Yuwono ◽  
Oky Haribudiman
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Agents ◽  
Agar Plate ◽  
Antibacterial Properties ◽  
Water Extract ◽  
Inhibition Zone ◽  
Coffea Canephora ◽  
Healthcare Associated Infections ◽  
Robusta Coffee ◽  
Healthcare Associated

Pseudomonas aeruginosa umum ditemukan pada Healthcare-associated infections (HAIs) dan menunjukkan resistensi pada banyak agen antimikrob yang umum. Kopi Robusta telah dipilih sebagai antibiotik alternatif karena memiliki kemampuan antibakteri. Penelitian ini bertujuan mengetahui aktivitas antibakteri ekstrak air kopi robusta (Coffea canephora) terhadap bakteri Pseudomonas aeruginosa. Penelitian dilakukan di Laboratorium Mikrobiologi dan Parasitologi Fakultas Kedokteran UNPAD periode Maret – Desember 2020. Perlakuan yang diberikan adalah media agar diberi disk yang mengandung ekstrak air kopi robusta dalam 4 dosis, yaitu 12,5%, 25%, 50%, dan 100%, imipenem (kontrol positif), dan disk yang mengandung aquadest (kontrol negatif). Agar diinkubasi dalam inkubator dengan suhu 37ºC selama 24 jam. Penilaian dilakukan dengan pengamatan dan pengukuran zona inhibisi. Data hasil penelitian membuktikan zona inhibisi yang dihasilkan oleh ekstrak air kopi robusta pada dosis 100% memiliki rerata paling tinggi dibanding dengan dosis lainnya, yaitu 8,13 mm, namun lebih kecil dibanding dengan imipenem yang memiliki rerata zona inhibisi sebesar 25 mm. Hal ini menunjukkan bahwa ekstrak air kopi robusta memiliki efek antibakteri terhadap Pseudomonas aeruginosa. Antibacterial Activities of Robusta Coffee Water Extract (Coffea Canephora) against Pseudomonas aeruginosa BacteriaPseudomonas aeruginosa is common in Healthcare-associated infections (HAIs) and shows resistance to many common antimicrobial agents. Robusta coffee has been chosen as an alternative to antibiotics because of its antibacterial properties. This study aims to determine the antibacterial activity of Robusta coffee (Coffea canephora) water extract against Pseudomonas aeruginosa. The research was done in Laboratorium Mikrobiologi and Parasitologi Fakultas Kedokteran UNPAD period March – December 2020.The treatments given was agar plate that added Robusta coffee water extract (Coffea canephora) in 4 doses, namely 12.5%, 25%, 50%, and 100%, imipenem antibiotic disc (positive control), and disk containing aquadest (negative control). They were then incubated in an incubator at 37ºC for 24 hours. The assessment was carried out by observing and measuring the inhibition zone. The research data proved that the inhibition zone produced by robusta coffee water extract at a dose of 100% had the highest average compared to other doses, which is at 8.13 mm, but smaller than imipenem which had an average inhibition zone of 25 mm. This shows that robusta coffee water extract has an antibacterial effect against Pseudomonas aeruginosa.

Evaluation of the Degree of Dental Anxiety in a Lot of Patients Altered in Young Adults

2019 ◽  
Vol 70 (2) ◽  
pp. 689-692
Author(s):  
Gheorghe Raftu ◽  
Elena-Claudia Sin ◽  
Aureliana Caraiane ◽  
Steliana Gabriela Bustiuc ◽  
Raluca Briceag
Keyword(s):  
Young Adults ◽  
Oral Cavity ◽  
Dental Treatment ◽  
Economic Status ◽  
Dental Anxiety ◽  
Background Level ◽  
Anxiety Scale ◽  
Statistical Relationships ◽  
And Gender ◽  
Age Range

The study aims at identifying and highlighting dental anxiety in a group of young adults, analyzing patients� concerns about the main dental treatment procedures, and establishing statistical relationships between anxiety and gender, the background, level of education, socio-economic status and knowledge of habits of care and hygiene of oral cavity characteristic of the studied group. The study group consists of 150 female (50%) and male (50%) patients , aged between 20 and 40 years. The inclusion criteria in the batch were those related to the age range of 20 to 40 years.Two respondents (Corah�s Dental Anxiety Scale, Revised (DAS-R)) were given two questionnaires ( Corah�s Dental Anxiety Scale, DAS-R), and the second questionnaire contained 7 questions about the habits dental care). Most patients� concerns about the main procedures of dental treatment / other concerns are: dental extraction, material expenses, possibility of treatments and shame she felt oppressed health of the oral cavity. Regardless of the level of dental anxiety, the oral cavity hygiene habits are not properly performed by patients, with an increased tendency for them to be disregarded by patients with severe dental anxiety.

scholarly journals Pseudomonas aeruginosa PA14 Enhances the Efficacy of Norfloxacin against Staphylococcus aureus Newman Biofilms

2020 ◽  
Vol 202 (18) ◽  
Author(s):  
Giulia Orazi ◽  
Fabrice Jean-Pierre ◽  
George A. O’Toole
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Agents ◽  
Respiratory Infections ◽  
Multiple Infection ◽  
Bacterial Biofilms ◽  
Interspecies Interactions ◽  
Chronic Infections ◽  
Content Type

ABSTRACT The thick mucus within the airways of individuals with cystic fibrosis (CF) promotes frequent respiratory infections that are often polymicrobial. Pseudomonas aeruginosa and Staphylococcus aureus are two of the most prevalent pathogens that cause CF pulmonary infections, and both are among the most common etiologic agents of chronic wound infections. Furthermore, the ability of P. aeruginosa and S. aureus to form biofilms promotes the establishment of chronic infections that are often difficult to eradicate using antimicrobial agents. In this study, we found that multiple LasR-regulated exoproducts of P. aeruginosa, including 2-heptyl-4-hydroxyquinoline N-oxide (HQNO), siderophores, phenazines, and rhamnolipids, likely contribute to the ability of P. aeruginosa PA14 to shift S. aureus Newman norfloxacin susceptibility profiles. Here, we observe that exposure to P. aeruginosa exoproducts leads to an increase in intracellular norfloxacin accumulation by S. aureus. We previously showed that P. aeruginosa supernatant dissipates the S. aureus membrane potential, and furthermore, depletion of the S. aureus proton motive force recapitulates the effect of the P. aeruginosa PA14 supernatant on shifting norfloxacin sensitivity profiles of biofilm-grown S. aureus Newman. From these results, we hypothesize that exposure to P. aeruginosa PA14 exoproducts leads to increased uptake of the drug and/or an impaired ability of S. aureus Newman to efflux norfloxacin. Surprisingly, the effect observed here of P. aeruginosa PA14 exoproducts on S. aureus Newman susceptibility to norfloxacin seemed to be specific to these strains and this antibiotic. Our results illustrate that microbially derived products can alter the ability of antimicrobial agents to kill bacterial biofilms. IMPORTANCE Pseudomonas aeruginosa and Staphylococcus aureus are frequently coisolated from multiple infection sites, including the lungs of individuals with cystic fibrosis (CF) and nonhealing diabetic foot ulcers. Coinfection with P. aeruginosa and S. aureus has been shown to produce worse outcomes compared to infection with either organism alone. Furthermore, the ability of these pathogens to form biofilms enables them to cause persistent infection and withstand antimicrobial therapy. In this study, we found that P. aeruginosa-secreted products dramatically increase the ability of the antibiotic norfloxacin to kill S. aureus biofilms. Understanding how interspecies interactions alter the antibiotic susceptibility of bacterial biofilms may inform treatment decisions and inspire the development of new therapeutic strategies.

scholarly journals 1597. Ceftolozane-Tazobactam and Meropenem Synergy Testing Against Multi-Drug and Extensively-Drug Resistant Pseudomonas aeruginosa

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S794-S795
Author(s):  
Mary Francine P Chua ◽  
Syeda Sara Nida ◽  
Jerry Lawhorn ◽  
Janak Koirala
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Synergistic Effect ◽  
Inhibitory Concentration ◽  
Multidrug Resistant ◽  
Additive Effect ◽  
Teaching Hospitals ◽  
Drug Resistant ◽  
Extensively Drug Resistant ◽  
Synergy Testing ◽  
Concentration Indices

Abstract Background Multidrug-resistant (MDR) and extensively drug-resistant (XDR) Pseudomonas aeruginosa (PA) have limited therapeutic options for treatment. Ceftolozane/tazobactam is a newer anti-pseudomonal drug effective against resistant PA infections, however resistance against this drug has now also developed and is increasing. In this study, we explored the combination of ceftolozane/tazobactam (CT) and meropenem (MP) as a possible effective regimen against MDR and XDR PA. Methods We obtained 33 non-duplicate isolates of MDR and XDR PA grown from blood, urine and respiratory samples collected from patients admitted between 2015 and 2019 at our two affiliate teaching hospitals. MDR PA was defined as resistance to 3 or more classes of anti-pseudomonal antibiotics, and XDR PA as resistance to all but two or less classes of anti-pseudomonal antibiotics. Antimicrobial preparations of both MP and CT were made according to manufacturer instructions. Susceptibility testing was performed using the checkerboard method in accordance to CLSI guidelines (CLSI M100, 2017). The ATCC 27853 strain of PA used as control. Synergy, additive effect, indifference and antagonism were defined as FIC (fractional inhibitory concentration) indices of ≤0.5, &gt;0.5 to &lt;1, &gt;1 to &lt;4, and &gt;4, respectively. Results Thirteen (39%) of 33 PA isolates were classified as XDR, while 20 (61%) PA isolates were MDR. All isolates were resistant to MP (MIC50 &gt;32 ug/mL), while only 2 (6%) isolates were susceptible to CT (MIC50 64 ug/mL). A synergistic effect was seen in 9 (27.3%) of PA isolates (FIC index range 0.28 to 0.5)— 2 of which were XDR PA, and 7 were MDR PA. An additive effect was seen in 12 (36.4%), with indifference seen in 12 (36.4%) of isolates. In this study, no antagonism was seen when CT and MP were combined. Conclusion When used in combination, CT and MP can exert a synergistic effect against MDR and XDR PA. Additive effect and indifference can also be seen when both antibiotics were used. Moreover, there was no antagonism seen when both antibiotics were combined. This study shows that the use of CT and MP in combination may be an option against XDR and MDR PA infections. Disclosures All Authors: No reported disclosures

Sign in / Sign up

Export Citation Format

Share Document