Efficiency of Grape (Vitis vinifera) Seed Oil on Nonspecific Immune Response and Histopathological Effects in Common Carp Cyprinus carpio Challenged with Pseudomonas aeruginosa

2021 ◽  
Vol 5 (1) ◽  
pp. 130-140
Author(s):  
Israa Al-Atbee
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cyprinus Carpio ◽  
Phagocytic Activity ◽  
Seed Oil ◽  
Grape Seed ◽  
Lysozyme Activity ◽  
Control Group ◽  
Treatment Groups ◽  
Grape Seed Oil ◽  
Histopathological Effects

The present study was carried out to assess the effect of grape seed oil supplemented diet on non- specific immunity of Cyprinus carpio against the pathogenic bacteria Pseudomonas aeruginosa. Fish weights 41.69±2.25 g and total length 13.65±1.24 cm were randomly distributed into four treatments (two replications for each treatment). Fish groups were fed four dissimilar diets up to 40 days. The first group (T1) was fed with 0.2% grape seed oil, the second group (T2) was fed supplemented with 0.5% grape seed oil, the third group (T3) was supplemented with 1% grape seed oil and the forth group (T4) was served as the control group which fed basal diet without supplementing with grape seed oil. Forty-six fishes in all treatment groups were challenged intramuscularly with P. aeruginosa on day 41. The fishes in experimental groups were challenged intramuscularly with 0.2 ml P. aeruginosa at a concentration of 108 CFU/ml and after challenge (14 days), the different parameters were determined including nitroblue tetrazolium % (NBT), myeloperoxidases % (MPO), phagocytic activity (%) and serum lysozyme activity (U/min). The results indicated that T2 group had significant increased (p<0.05) in NBT activity, MPO activity, phagocytic activity and lysozyme activity compared with control group and to other treatment groups, followed by T1 and T3, respectively. In addition, T2 showed highest resistance to challenge P. aeruginosa compared with other groups. T3 and T4 groups showed a decreased performance in all non-specific immune parameters. There were histopathological effects in liver, showing a focal region of lymphocyte aggregation (T3), and hemorrhage into the hepatic vein with infiltration of inflammatory cells (T4). Therefore, these results indicated that 0.5% grape seed oil (T2) as additive fed could be used as prophylactic in common carp culture to enhance the protection against any possible infection by P. aeruginosa.

scholarly journals Effect of Grape Seed Oil on Chronic Carbon Tetrachloride-Induced Hepatic Injury and Determination of Hepatic Apoptosis in Rats

2019 ◽  
pp. 1-10
Author(s):  
Ayhan Atasever ◽  
Ahmet Alpay ◽  
Gorkem Ekebas ◽  
Duygu Yaman Gram
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatic Injury ◽  
Caspase 3 ◽  
Seed Oil ◽  
Antioxidant Properties ◽  
Elevated Serum ◽  
Grape Seed ◽  
Control Group ◽  
Albino Rats ◽  
Grape Seed Oil

Aims: The present study was designed to evaluate the hepatoprotective activity of grape seed oil (GSO) on liver lesions induced by carbon tetrachloride (CCl4) in rats. The effects on hepatic injury were investigated by measuring serum levels of ALT, triglyceride, total protein, total cholesterol and liver levels of MDA. Furthermore, caspase -3, -8 and -9 activities in cellular apoptosis were determined. Place and Duration of Study: Faculty of Veterinary Medicine, Department of Pathology, Erciyes University, Kayseri, between November 2017 and September 2018. Methodology: In this study 40 male Wistar albino rats were divided into four groups including 10 animals in each. Control group administered with 0.9% NaCl. The second group was administered with 4 mL/kg GSO for twelve weeks. Third group were given CCl4 (0.2 mL/kg) twice for 8-weeks. Fourth group was administered with 4 mL/kg GSO, for 12 weeks and also given CCl4 (0.2 mL/kg) twice for 8 weeks, starting from the 5th week. Results: Histopathological examination of CCl4 group showed intense macro and micro vesicular steatosis in hepatocytes, necrosis, and lymphocytes rich mononuclear cell infiltration in portal area and mild portal fibrosis in the parenchyma. The grape seed oil applications have partially normalized the altered histological changes and the activity of caspase -3, -8 and -9. Administration of GSO led to a decline in the activities of ALT and MDA levels while this treatment elevated serum triglycerides levels which are not significantly important. Conclusion: The results indicate that the antioxidant properties of GSO have not ameliorative effect in either the histopathological lesions or biochemical parameters against CCl4-induced hepatotoxicity in rats. Also, it has been concluded that duration‐dependent further research results are needed to determine the effects of grape seed oil in high doses which can give the best results without side effects.

scholarly journals Comparing the Effect of Foot Massage with Grape Seed Oil and Sweet Almond Oil on Physiological Leg Edema in Primigravidae: A Randomized Clinical Trial

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Maryam Navaee ◽  
Marzieh Rakhshkhorshid
Keyword(s):  
Clinical Trial ◽  
Pregnant Women ◽  
Randomized Clinical Trial ◽  
Seed Oil ◽  
Grape Seed ◽  
Control Group ◽  
Almond Oil ◽  
Grape Seed Oil ◽  
Leg Edema ◽  
Sweet Almond

Background. Leg edema is a prevalent problem in pregnancy causing activity restrictions for pregnant women. This study was performed to compare the effect of foot massage using grape seed oil and sweet almond oil on physiological leg edema. Methods. A randomized clinical trial was conducted on 90 primigravidae referred to public health centres of Zahedan, Iran. The participants’ gestational age was 30–40 weeks. The study was conducted from August 2016 to November 2017. The participants were randomly assigned to three groups (massage with grape seed oil, massage with sweet almond oil, and without intervention). After determining the extent of leg edema, foot massages were done for 20 minutes within 5 days in the two intervention groups. Then, foot circumferences were measured on day 5 after the intervention. Foot circumferences for the control group were measured on days 1 and 5. A nonelastic tape measure was used to measure the circumferences. To analyse the data, SPSS 21 software and statistical tests including one-way ANOVA, Tukey’s test, and paired t-test were used. Results. The results from this study showed a significant difference in the mean score change of foot circumferences between groups (P=0.001). According to the results of Tukey’s test, mean score changes of foot circumferences of both intervention groups were significantly different those of the control group. However, this difference was not significant between the two intervention groups (P=0.865). Conclusion. The findings of this study confirmed the effectiveness of foot massage using grape seed and sweet almond oils to reduce pregnancy physiological edema. Therefore, foot massage with appropriate oils can be used as a useful technique by trained midwives in prenatal care centres or at pregnant women houses. This trial is registered with IRCT2015072723370N1.

scholarly journals AMELIORATIVE EFFECTS OF GRAPE SEED OIL ON CHROMIUM-INDUCED NEPHROTOXICITY AND OXIDATIVESTRESS IN RATS

2020 ◽  
Vol 57 (3) ◽  
Author(s):  
Sahar Hassan Orabi ◽  
Sherif Mohamed Shawky
Keyword(s):  
Dna Damage ◽  
Hexavalent Chromium ◽  
Seed Oil ◽  
Potassium Dichromate ◽  
Serum Levels ◽  
Grape Seed ◽  
Renal Tissue ◽  
Male Rats ◽  
Group I ◽  
Grape Seed Oil

The current study focused on investigating the renoprotective effects of grape seed oil (GSO) against hexavalent chromium (Cr (VI))-induced nephrotoxicity. A total of 40 male rats were randomly divided into four groups: group I served as the control group, group II received 1000 mg/L potassium dichromate (353.5 mg/L Cr(VI)) in drinking water for 12 weeks, group III received 3.7 g/kg body weight/day GSO orally for 12 weeks, and group IV received GSO together with potassium dichromate for 12 weeks. Cr(VI) significantly increased serum levels of urea, creatinine, potassium and glucose. In addition, Cr(VI) increased MDA levels and induced renal tissue damage and DNA damage. On the other hand, Cr(VI) decreased serum levels of sodium and antioxidant defence system [reduced glutathione (GSH) and catalase (CAT)]. However, treatment with GSO prevented elevation levels of serum urea, creatinine, potassium and glucose. In addition, GSO enhanced sodium level, renal tissue antioxidant defense system due to its curative effect ameliorated particularly oxidative stress, renal tissue and DNA damage. In conclusion, these results demonstrate that GSO is a promising nephroprotective agent against Cr(VI)-induced nephrotoxicity.Key words: grape seed oil; hexavalent chromium; nephrotoxicity; DNA damage BLAŽILNI UČINKI OLJA GROZDNIH PEŠK PRI TOKSIČNI OBREMENITVI LEDVIC TER VPLIV NA OKSIDATIVNI STRES PODGAN, POVZROČEN S KROMOM Povzetek: Študija je bila osredotočena na proučevanje zaščitnih učinkov olja grozdnih pešk (GSO) pri toksični obremenitvi ledvic, povzročeni s heksavalentnim kromom (Cr (VI)). Štirideset samcev podgan je bilo naključno razdeljenih v štiri skupine: skupina I - kontrolna skupina, skupina II, ki je v pitni vodi 12 tednov prejemala 1000 mg/L kalijevega dikromata (353,5 mg/L Cr (VI)), skupina III, ki je peroralno 12 tednov prejemala 3,7 g/kg telesne mase/dan GSO ter skupina IV, ki je 12 tednov prejemala GSO skupaj s kalijevim dikromatom. Cr(VI) je znatno zvišal serumske ravni sečnine, kreatinina, kalija in glukoze v serumu. Poleg tega je Cr(VI) zvišal raven MDA in povzročil poškodbe ledvičnega tkiva in poškodbe DNK. Po drugi strani je Cr(VI) znižal serumsko raven natrija in antioksidativnega obrambnega sistema, zmanjšal raven glutationske peroksidaze in katalaze. Dodajanje GSO poskusnim živalim je preprečilo zvišanje ravni sečnine v serumu, kreatinina, kalija, natrija in glukoze. Poleg tega je GSO izboljšal obrambni sistem antioksidantov ledvičnega tkiva. Zaradi svojega zdravilnega učinka je izboljšal zlasti oksidativni stres, poškodbe ledvičnega tkiva in DNK. Rezultati kažejo, da je GSO obetavno zaščitno sredstvo za ledvica pri toksični obremenitvi, povzročeni s Cr(VI).Ključne besede: olje grozdnih pešk; heksavalentni krom; nefrotoksičnost; poškodba DNK

scholarly journals Characterization of fatty acid, antioxidant, and polyphenol content of grape seed oil from different Vitis vinifera L. varieties

OCL ◽  
2021 ◽  
Vol 28 ◽  
pp. 30
Author(s):  
Viktória Kapcsándi ◽  
Erika Hanczné Lakatos ◽  
Beatrix Sik ◽  
László Ádám Linka ◽  
Rita Székelyhidi
Keyword(s):  
Seed Oil ◽  
Grape Seed ◽  
Seed Oils ◽  
Grape Seeds ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Total Polyphenol Content ◽  
Grape Seed Oil ◽  
Total Antioxidant ◽  
Grape Varieties

In this study, we examined the yield and oil quality of eight different grape varieties. For the experiments, the grape seeds were obtained from the Benedictine Pannonhalma Archabbey in the northwestern region of Hungary. The aim of the studies was to determine the oil yield obtained by extraction and to examine the differences between the fatty acid composition, antioxidant capacity, and total polyphenol content of the oils of different grape varieties. Based on the results, the oil content of the grape seeds varied between 99.91 g/kg and 126.74 g/kg. The grape seed oils analysed contained significant amounts of stearic acid (3.42–9.93%), palmitic acid (7.81–10.66%), oleic acid (14.29–19.92%), and linoleic acid (66.85–72.47%). Besides, the grape seed oils tested contained several other fatty acids in small amounts. There were significant differences in the total antioxidant and total polyphenol content of the oils. Total polyphenol content ranged from 0.24 to 1.13 mg GAE/g, while the total antioxidant content changed between 0.12 and 0.78 μg TEAC/g. The results show that the studied varieties are suitable for the production of table grape seed oil based on their oil yield, and the oils have favourable, health-protecting properties in terms of their quality.

scholarly journals Protective effects of vitamin E and grape seed oil against acute hepatorenal ivermectin toxicity in mice: biochemical and histopathological studies

2019 ◽  
Vol 7 (2) ◽  
pp. 087-094
Author(s):  
Mai Mohsen El Maghraby ◽  
Nada Mosaad El Maghraby ◽  
Ameera Ahmed Salama ◽  
Azza Shawky Abdlmonem ◽  
Eman Abdellatefe Authman ◽  
...  
Keyword(s):  
Vitamin E ◽  
Seed Oil ◽  
Grape Seed ◽  
Protective Effects ◽  
Grape Seed Oil ◽  
Histopathological Studies

scholarly journals The cell protection of weated pigs for probiotics

2020 ◽  
pp. 111-119
Author(s):  
L. Bondarenko
Keyword(s):  
Immune System ◽  
Blood Serum ◽  
Phagocytic Activity ◽  
Lysozyme Activity ◽  
The State ◽  
Natural Resistance ◽  
Phagocytic Index ◽  
Control Group ◽  
Stressful Situation ◽  
Experimental Group

The immune system is central to ensuring the consistency of the body's homeostasis. The state of the body's natural resistance is determined by a set of non-specific protective mechanisms. Lymphocytes and phagocytes are actively involved in maintaining immunity. Lymphocytes recognize the antigens of pathogenic microorganisms, and phagocytes absorb and destroy the pathogens themselves. During the weaning of piglets from sows there is a decrease in the protective forces of their body. During this period, the natural resistance of the piglets is reduced due to the stressful situation caused by changing conditions of confinement, the transition to full feed and lack of sows. The immune system of weaning pigs is relatively weak, so when exposed to environmental and technological stressors, they become susceptible to various diseases. The use of probiotic drugs stimulates the activity of the immune system, prevents stress and immunodeficiency. One of these probiotics is the probiotic of domestic production Protecto-active. It w observed the the influence of the probiotic Protecto-active on the indices of nonspecific resistance of the young pigs organism to the growth. An increase in bactericidal activity of blood serum by 12.10% (P <0.05) and lysozyme activity of blood in the piglets of the experimental group was increased by 3.71% compared to control, which indicates the activation of the body's defenses and the increase in adaptive capacity. An important step in the study of the influence of the probiotic Protekto-active on the state of the immune system is to determine the phagocytic activity of neutrophils, phagocytic index and phagocytic number. In the experimental group of piglets that were fed the probiotic Protecto-active, we found an increase in leukocyte phagocytic activity by 9.0% (P <0.001), a phagocytic index by 51.7% (P<0.001) and a phagocytic number by 24.8% ( P <0.01) compared with the control group. Thus, using a probiotic Protecto-active, all indicators of phagocytosis increase: the number of phagocytes increases, their ability to capture microorganisms and increases their digestive capacity, it increases the bacterial and lysozyme activity of blood serum, which is positively reflected in the immunobiosity. Key words: probiotic, phagocytosis, phagocytic index, phagocytic number, phagocytic activity of leukocytes, cellular immunity, piglets

scholarly journals Effect of Gold Sea Cucumber (Stichopus hermanni) Extract Concentration on Antioxidant Activity of Grape Seed Oil (Vitis vinifera) nanoemulsion

2018 ◽  
Vol 16 (1) ◽  
pp. 36 ◽  
Author(s):  
Siti Umroh Noor ◽  
Amelia Gozali
Keyword(s):  
Antioxidant Activity ◽  
Sea Cucumber ◽  
Seed Oil ◽  
Grape Seed ◽  
Omega 3 ◽  
Extract Concentration ◽  
Globule Size ◽  
Grape Seed Oil ◽  
Dpph Method ◽  
Antioxidant Agent

Gold sea cucumber extract (Stichopus hermanni) is rich in collagen and unsatturated fatty acid also known omega 3 such as docosahexaenoic acid and eicosapentaenoic acid that highly effective as an antioxidant. Oligomeric proanthocyanidin is the main content in grape seed oil and the most powerful antioxidant agent that can protect collagen. The aim of research was to determine the effect of gold sea cucumber extract concetration on antioxidant activity of grape seed oil nanoemulsion. The three formulas with results test of antioxidant activity with DPPH method using 15% Brij CS12® and 30% propylene glycol has done which the oil phase was dispersed in the aqueous phase.Variation that have been done is to use the antioxidant activity (IC50) of gold sea cucumber extract  for 2, 4, and 6 times. The resulting preparations was conformed of organoleptic, nanoemulsion type, clarity, globule size, viscosity and rheology, pH and freez thaw test. Antioxidant activity of gold sea cucumber extract and grape seed oil were  (IC50= 53.79 and 17.41 μg/mL respectively). The nanoemulsion was analysed using two-way ANOVA method. Antioxidant  activity at storage for 0; 2 and 4-weeks of formula I, II, III were (IC50=  49.15; 49.45, 51.15 µg/mL), (IC50= 44.12, 47.48, 48.14 µg/mL), and (IC50= 42.14, 43.45, 45.33 µg/mL) respectively. Can be concluded that the higher concentration of gold sea cucumber extract, the antioxidant activity of nanoemulsion is higher, but it decreased during the storage.

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