scholarly journals The GC–MS analysis of the diethylether and ethylacetate fraction of the peel of Solanum incanum and the study of their antibacterial activity

2022 ◽  
Author(s):  
Yesudass Dominic Ravichandran ◽  
Mequanint Molla Yetayih
Keyword(s):  
Antibacterial Activity ◽  
Solanum Incanum ◽  
Ms Analysis

scholarly journals Extraction and GC-MS Analysis of the Essential Oil from the Peel of Solanum incanum and its Antibacterial Activity Studies

2020 ◽  
Vol 32 (8) ◽  
pp. 2001-2006
Author(s):  
Mequanint Molla Yetayih ◽  
Yesudass Dominic Ravichandran
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Diethyl Ether ◽  
Methanolic Extract ◽  
Positive Control ◽  
Gas Chromatography Mass Spectrometry ◽  
Mass Spectral ◽  
E Coli ◽  
Solanum Incanum ◽  
Ms Analysis

Phytochemicals were extracted from the peel of Solanum incanum (S. incanum) with methanol (70% v/v). The phytochemical screening of the methanolic extract showed the presence of glycosides, steroids, terpenoids, flavonoids, tannins, alkaloids, saponins and phenolic compounds. The successive partitioning of the methanolic extract was carried out with three solvents hexane, diethyl ether and ethyl acetate. The diethyl ether fraction yielded two immiscible fractions. The oil was separated as essential oil fraction and analyzed by GC-MS (gas chromatography-mass spectrometry). The GC-MS analysis of the essential oil indicated 17 compounds including 2,3-butanediol (76.76%), diethyl phthalate (8.32%), benzyl benzoate (3.02%), 2,6-dimethyl-6-nitro-2-hepten-4-one (2.56%) and 1,2-dimethoxy- 4-(1-propenyl)benzene (1.88%). Among them, the mass spectral patterns of three compounds were analyzed, discussed and confirmed with NIST database. The antibacterial study of essential oil was conducted using four pathogens (E. coli, K. pneumonia, S. aureus and S. pyogenes) at four different concentrations (250, 500, 750 and 1000 μg/mL). The result indicates that the essential oil extracted from the peel of Solanum incanum exhibited remarkable antibacterial activity against E. coli and K. pneumonia in 1000 μg/mL, respectively compared with the positive control gentamicin (10 μg/mL). The result of this study revealed the presence of various organic components and the antibacterial activity of this plant essential oil may be as a result of the major compounds.

scholarly journals Quorum Sensing Inhibition in Chromobacterium violaceum, Antibacterial Activity and GC-MS Analysis of Centaurea praecox (Oliv. & Hiern) Extracts

2020 ◽  
Vol 9 (4) ◽  
pp. 1569-1577
Keyword(s):  
Antibacterial Activity ◽  
Quorum Sensing ◽  
Research Strategy ◽  
Chromobacterium Violaceum ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Control Drug ◽  
Ms Analysis ◽  
Drug Resistant Bacteria

The quorum sensing (QS) mechanism has become a viable research strategy for the discovery of plant-derived anti-virulent agents to control drug-resistant bacteria. The increasing incidences of drug-resistant bacteria and the effort to curb it necessitate this study. We investigated the QS inhibitory potential of Centaurea praecox extracts on Chromobacterium violaceum (CV), antibacterial activity, and determination of chemical composition using GC-MS. C. praecox was subjected to sequential extraction using hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), ethanol (ET), and aqueous (AQ) solvents. The extracts were subsequently evaluated for antibacterial activity using disc diffusion and QS violacein inhibition using spectrophotometry. The antibacterial effects of the extracts were moderate on gram-positive bacteria at 4 mg/mL in the order: HEX >EA >DCM >ET =AQ. However, the DCM extract demonstrated the most effective violacein inhibition of ≥80% at 0.3 mg/mL. QS violacein inhibitions were generally found to be concentration-dependent in the order: DCM >EA >HEX >ET =AQ with efficacies of ≥ 90% inhibition at ≥ 0.6 mg/mL. GC-MS analysis on the most potent DCM extract revealed N-vinylmethanimine, N-ethyl formamide, and propanamide among components identified. We concluded that C. praecox DCM extract contains bioactive chemicals as QS inhibitors and potential anti-virulent agents capable of combating the pathogenicity of drug-resistant bacteria in vivo.

scholarly journals GC-MS Analysis and Antibacterial Activity of different Solvent Extracts of Shuteria involucrata

2018 ◽  
Vol 7 (2) ◽  
pp. 116-120
Author(s):  
Senthamizh Selvan N ◽  
◽  
Isaiah S ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Disc Diffusion ◽  
Plant Materials ◽  
Gram Negative ◽  
Good Antibacterial Activity ◽  
Ms Analysis ◽  
Diffusion Technique

The present study was focused to examine the presence of phytoconstituents in the ethanolic extract of Shuteria involucrata plant using GC-MS analysis and Antibacterial activity. The GC-MS analysis of S. involucrata leaf was performed using Agilent 6890-JEOL GC-Mate-II Mass Spectrometer. The result of the study showed the presence of six bioactive compounds in the ethanolic extract. The antimicrobial activity was carried out by disc diffusion technique against the four selected pathogens. Among the four, tested for Antibacterial Activity Staphylococcus aureus, and Pseudomonas aeruginosa and were more susceptible to the extract, whereas the others are less susceptible. Ethanol and methanol extracts of plant materials exhibited good antibacterial activity against gram positive, gram negative bacterias

scholarly journals UHPLC-HESI-OT-MS-MS Biomolecules Profiling, Antioxidant and Antibacterial Activity of the “Orange-Yellow Resin” from Zuccagnia punctata Cav.

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 123 ◽  
Author(s):  
Jessica Gómez ◽  
Mario J. Simirgiotis ◽  
Sofía Manrique ◽  
Beatriz Lima ◽  
Jorge Bórquez ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Liquid Chromatography ◽  
Metabolite Profiling ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Ultrahigh Resolution ◽  
Antioxidant Power ◽  
Orange Yellow ◽  
Ms Analysis ◽  
Orbitrap Ms

This research was designed to investigate the metabolite profiling, phenolics, and flavonoids content as well as the potential antioxidant and antibacterial, properties of orange-yellow resin from Zuccagnia punctata Cav (ZpRe). Metabolite profiling was obtained by a ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-ESI-OT-MS-MS). The antioxidant properties were screened by four methods: 2,2-diphenyl-1-picrylhydrazyl assay (DPPH), trolox equivalent antioxidant activity assay (TEAC), ferric-reducing antioxidant power assay (FRAP), and lipid peroxidation in erythrocytes (LP)). The antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) rules. The resin displayed a strong DPPH scavenging activity (IC50 = 25.72 µg/mL) and showed a percentage of inhibition of LP close to that of the reference compound catechin (70% at 100 µg ZpRe/mL), while a moderated effect was observed in the FRAP and TEAC assays. The resin showed a content of phenolic and flavonoid compounds of 391 mg GAE/g and 313 mg EQ/g respectively. Fifty phenolics compounds were identified by ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-PDA-OT-MS) analysis. Thirty-one compounds are reported for the first time, updating the knowledge on the chemical profile of this species. The importance of the biomolecules identified support traditional use of this endemic plant. Furthermore, additional pharmacological data is presented that increase the potential interest of this plant for industrial sustainable applications.

scholarly journals Chemical Composition and in Vitro Antibacterial Activity of the Essential Oil of Calycolpus Moritzianus (O. Berg) Burret from Mérida, Venezuela

2008 ◽  
Vol 3 (6) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Teresa Díaz ◽  
Flor D. Mora ◽  
Judith Velasco ◽  
Tulia Díaz ◽  
Luis B. Rojas ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Enterococcus Faecalis ◽  
Chemical Constituents ◽  
Ms Analysis ◽  
In Vitro Antibacterial Activity

The chemical constituents of the essential oil obtained by hydrodistillation from the leaves of Calycolpus moritzianus (O. Berg) Burret, syn Psidium caudatum Mc Vaught, collected in November 2006 in Mérida State, Venezuela, were identified by GC-MS analysis. Thirty components (91.1% of the sample) were identified, of which the seven major ones were β-caryophyllene (21.9%), α-pinene (10.9%), viridiflorol (9.7%), β-selinene (6.1%), α-copaene (6.3%), α-selinene (5.3%) and γ-eudesmol (5.1%). The oil was found to have antibacterial activity against Staphylococcus aureus ATCC (6538) and Enterococcus faecalis ATCC (29212), with MIC values of 60 μg/mL and 180 μg/mL, respectively.

scholarly journals Gc-Ms Analysis and Antibacterial Activity of Mangosteen Leaf Extracts against Plant Pathogenic Bacteria

2016 ◽  
Vol 07 (07) ◽  
pp. 1013-1020 ◽  
Author(s):  
Qamar Mohammed Naji Alsultan ◽  
Kamaruzaman Sijam ◽  
Tavga Sulaiman Rashid ◽  
Khairulmazmi Bin Ahmad
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Leaf Extracts ◽  
Plant Pathogenic Bacteria ◽  
Ms Analysis

Antioxidant and wound healing potential of essential oil from Citrus reticulata peel and its chemical characterization. Medicinal values of peels essential oil

2020 ◽  
Vol 21 ◽  
Author(s):  
Maham Ishfaq ◽  
Bushra Akhtar ◽  
Faqir Muhammad ◽  
Ali Sharif ◽  
Muhammad Furqan Akhtar ◽  
...  
Keyword(s):  
Wound Healing ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Chemical Characterization ◽  
Total Flavonoid ◽  
Citrus Reticulata ◽  
Fruit Peel ◽  
Phenolic Contents ◽  
Ms Analysis

Background: Fruit peels are considered as waste and contribute towards a major proportion of biomass. They might be a good source of various therapeutic benefits. Peels biomass of citrus fruits are usually considered as garbage. Such peels may have many important and valuable medicinal components with pharmacological activities. Citrus reticulata, (of family Rutaceae, local name tangerine) is a local seasonal fruit in Pakistan which is a very good example regarding wastage of its peels. Objective: The study is based on exploration of a citrus fruit peel derived essential oil, its chemical characterization, identification of various bioactive components and the exploration of pharmacological potentials (antibacterial and wound healing activity). Method: Essential oil was recovered by hydro-distillation of freshly collected peels. Chemical constituents of oil were determined by gas chromatography-mass spectroscopy (GC-MS) analysis. Antioxidant activities were evaluated by total phenolic contents, total flavonoid content, DPPH scavenging activity and reducing power assay. Antibacterial activity was determined using disc diffusion assay. In vivo wound healing potential was determined in rabbits after topical administration of oil. Wound scoring was calculated followed by histological study. Results: GC-MS analysis showed presence of various components with greatest proportion of D-Limonene (89.31%). Total flavonoid and phenolic contents were found to be 14.63 ± 0.95 mg CE/g and 17.03 ± 3.24 mg GAE/g respectively while DPPH activity was found to be 73.32%. Better antibacterial activity was shown against E. coli. In vivo studies showed significant reduction in wound diameter in essential oil treatment groups. Further the essential oil was found non-irritant in draize scoring. Conclusion: The study concluded that essential oil of this fruit peel might be used for antibacterial and wound healing purposes.

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