Ultraviolet induced random mutagenesis in Bacillus amyloliquefaciens (MF 510169) for improving biodiesel production

Fuel ◽  
2021 ◽  
Vol 304 ◽  
pp. 121380
Author(s):  
Amruta Prakash ◽  
Chandrahasya Namdev Khobragade ◽  
Rajaram Sakharam Mane
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Random Mutagenesis ◽  
Biodiesel Production

scholarly journals Random- Mutagenesis in Photosynthetic Microorganisms Further Selected with Respect to Increased Lipid Content

2018 ◽  
Vol 1 (1) ◽  
pp. 501-507
Author(s):  
Ana Valentina Ardelean ◽  
Ioan I. Ardelean ◽  
Oana Alina Sicuia-Boiu ◽  
Petruţa Cornea
Keyword(s):  
Genetic Diversity ◽  
Lipid Content ◽  
Random Mutagenesis ◽  
Biodiesel Production ◽  
Biotechnological Applications ◽  
Omega 3 ◽  
Naturally Occurring ◽  
Induced Mutagenesis ◽  
Photosynthetic Microorganisms ◽  
Selection Of

Abstract In the last decade there is an increased interest in selecting photosynthetic microorganisms with higher lipid content useful for biotechnological applications. In this paper we present our original results concerning: i) the selection of naturally occurring photosynthetic microorganisms with higher lipid content; ii) the use of these isolates as biological material subject of randomly induced mutagenesis; iii) selection by iodine vapour method of clones with decreased polysaccharides content and expected higher lipid content) as well as iv) genetic analysis of most promising strains in order to check the if they are true mutants or not. The results thus obtained argue the importance of the selection of naturally occurring photosynthetic microorganisms with higher lipid content as well as the use of random mutagenesis as a valuable tool to improve the genetic diversity of photosynthetic microorganisms in order to increase their ability to synthesize lipids for further biodiesel production and/or omega 3 or 6 production.

scholarly journals Protein Engineering by Random Mutagenesis and Structure-Guided Consensus of Geobacillus stearothermophilus Lipase T6 for Enhanced Stability in Methanol

2013 ◽  
Vol 80 (4) ◽  
pp. 1515-1527 ◽  
Author(s):  
Adi Dror ◽  
Einav Shemesh ◽  
Natali Dayan ◽  
Ayelet Fishman
Keyword(s):  
Protein Engineering ◽  
Organic Solvents ◽  
Random Mutagenesis ◽  
Acid Methyl Ester ◽  
Biodiesel Production ◽  
Geobacillus Stearothermophilus ◽  
Screening Assay ◽  
Lipase Gene ◽  
Improved Stability ◽  
Enhanced Stability

ABSTRACTThe abilities of enzymes to catalyze reactions in nonnatural environments of organic solvents have opened new opportunities for enzyme-based industrial processes. However, the main drawback of such processes is that most enzymes have a limited stability in polar organic solvents. In this study, we employed protein engineering methods to generate a lipase for enhanced stability in methanol, which is important for biodiesel production. Two protein engineering approaches, random mutagenesis (error-prone PCR) and structure-guided consensus, were applied in parallel on an unexplored lipase gene fromGeobacillus stearothermophilusT6. A high-throughput colorimetric screening assay was used to evaluate lipase activity after an incubation period in high methanol concentrations. Both protein engineering approaches were successful in producing variants with elevated half-life values in 70% methanol. The best variant of the random mutagenesis library, Q185L, exhibited 23-fold-improved stability, yet its methanolysis activity was decreased by one-half compared to the wild type. The best variant from the consensus library, H86Y/A269T, exhibited 66-fold-improved stability in methanol along with elevated thermostability (+4.3°C) and a 2-fold-higher fatty acid methyl ester yield from soybean oil. Based onin silicomodeling, we suggest that the Q185L substitution facilitates a closed lid conformation that limits access for both the methanol and substrate excess into the active site. The enhanced stability of H86Y/A269T was a result of formation of new hydrogen bonds. These improved characteristics make this variant a potential biocatalyst for biodiesel production.

scholarly journals Improved Raoultella planticola Strains for the Production of 2,3-Butanediol from Glycerol

Fermentation ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 11 ◽  
Author(s):  
Daniel Bustamante ◽  
Silvia Segarra ◽  
Alejandro Montesinos ◽  
Marta Tortajada ◽  
Daniel Ramón ◽  
...  
Keyword(s):  
Uv Light ◽  
Random Mutagenesis ◽  
Ultra Violet ◽  
Biodiesel Production ◽  
Product Concentration ◽  
Raw Glycerol ◽  
Raoultella Planticola ◽  
Process Economics ◽  
Mutant Strains ◽  
Class 1

Raw glycerol is an industrial byproduct from biodiesel production and is one of the most promising substrates for 2,3-butanediol (2,3-BD) production; however, 2,3-BD is not yet produced by fermentation from glycerol on a commercial scale due to poor process economics. Class 1 microorganism collections were screened and Raoultella planticola strain CECT 843 proved to be the best 2,3-BD producer, achieving (23.3 ± 1.4) g 2,3-BD per L and a yield of 36% (g 2,3-BD per g glycerol). To further increase product concentration and yield, R. planticola CEC T843 was subjected to random mutagenesis using ultra-violet (UV) light and ethyl methane sulfonate (EMS). Two mutant strains were found to produce at least 30% more 2,3-BD than the wild type: R. planticola IA1 [(30.8 ± 3.9) g 2,3-BD per L and 49% yield] and R. planticola IIIA3 [(30.5 ± 0.4) g 2,3-BD per L and 49% yield].

Biodiesel production from non-edible oils: A review

2020 ◽  
pp. 149-159
Author(s):  
Jatinder Kataria ◽  
Saroj Kumar Mohapatra ◽  
Amit Pal
Keyword(s):  
Environmental Impact ◽  
Energy Demand ◽  
Fossil Fuels ◽  
World Wide ◽  
Edible Oils ◽  
Control Measure ◽  
Biodiesel Production ◽  
Pollution Level ◽  
Animal Fats ◽  
The World

The limited fossil reserves, spiraling price and environmental impact due to usage of fossil fuels leads the world wide researchers’ interest in using alternative renewable and environment safe fuels that can meet the energy demand. Biodiesel is an emerging renewable alternative fuel to conventional diesel which can be produced from both edible and non-edible oils, animal fats, algae etc. The society is in dire need of using renewable fuels as an immediate control measure to mitigate the pollution level. In this work an attempt is made to review the requisite and access the capability of the biodiesel in improving the environmental degradation.

PENGARUH PELARUT KLOROFORM DALAM PEMURNIAN GLISEROL DENGAN PROSES ASIDIFIKASI ASAM KLORIDA

2016 ◽  
Vol 5 (3) ◽  
pp. 38-43
Author(s):  
Windi Monica Surbakti ◽  
Gerson Rico M.H ◽  
Mersi Suriani Sinaga
Keyword(s):  
Fatty Acid ◽  
Volume Ratio ◽  
Biodiesel Production ◽  
Extraction Time ◽  
Glycerol Concentration ◽  
Purification Process ◽  
Soap Content ◽  
Test Volume ◽  
Base Content ◽  
Water Salt

Glycerol as a byproduct of biodiesel production was approximately formed 10% of the biodiesel weight. Impurities which contained in the glycerol such as catalyst, soap, methanol, water, salt, and matter organic non glycerol (MONG) have a significant effect on the glycerol concentration. So, it is necessary to treat the impurities. The purpose of this study is to know the effect of chloroform to glycerol purification process with acidification method using hydrochloric acid as pretreatment process. This research was begun with acid addition to the glycerol to neutralize the base content and to split the soap content into free fatty acid and salt, that are more easily separated from glycerol. Then the process was continued with extraction by the solvent chloroform using the variable of test volume ratio (v/v) (1:1, 1:1.5, 1:2)  and the extraction time (20, 40, and 60 minutes). The results showed that the more volume of solvent used, gave less extraction time to produce high purity of glycerol. The highest purity produced in this study amounted to 90,9082% is obtained at the ratio of the volume solvent (v/v) 1:1 with extraction time 60 minutes.

Biodiesel Production from Microalgae

2013 ◽  
Vol 2 (2) ◽  
Author(s):  
M. Mohammadi ◽  
M. Azizollahi-Aliabadi
Keyword(s):  
Biodiesel Production

Directed Evolution of P450 Fatty Acid Decarboxylases via High-Throughput Screening Towards Improved Catalytic Activity

2019 ◽  
Author(s):  
Huifang Xu ◽  
Weinan Liang ◽  
Linlin Ning ◽  
Yuanyuan Jiang ◽  
Wenxia Yang ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Fatty Acid ◽  
Directed Evolution ◽  
High Throughput ◽  
High Throughput Screening ◽  
Colorimetric Detection ◽  
Screening Method ◽  
Random Mutagenesis ◽  
Direct Production ◽  
Consumption Activity

P450 fatty acid decarboxylases (FADCs) have recently been attracting considerable attention owing to their one-step direct production of industrially important 1-alkenes from biologically abundant feedstock free fatty acids under mild conditions. However, attempts to improve the catalytic activity of FADCs have met with little success. Protein engineering has been limited to selected residues and small mutant libraries due to lack of an effective high-throughput screening (HTS) method. Here, we devise a catalase-deficient <i>Escherichia coli</i> host strain and report an HTS approach based on colorimetric detection of H<sub>2</sub>O<sub>2</sub>-consumption activity of FADCs. Directed evolution enabled by this method has led to effective identification for the first time of improved FADC variants for medium-chain 1-alkene production from both DNA shuffling and random mutagenesis libraries. Advantageously, this screening method can be extended to other enzymes that stoichiometrically utilize H<sub>2</sub>O<sub>2</sub> as co-substrate.

PHÂN LẬP VÀ TUYỂN CHỌN CÁC CHỦNG BACILLUS ỨC CHẾ VI KHUẨN VIBRIO PARAHAEMOLYTICUS GÂY BỆNH TÔM CHẾT SỚM Ở TỈNH THỪA THIÊN HUẾ

2015 ◽  
Vol 100 (1) ◽  
Author(s):  
Nguyễn Thị Bích Đào ◽  
Trần Quang Khánh Vân ◽  
Nguyễn Văn Khanh ◽  
Nguyễn Quang Linh
Keyword(s):  
Bacillus Subtilis ◽  
Vibrio Parahaemolyticus ◽  
Bacillus Amyloliquefaciens ◽  
Bacillus Spp ◽  
Bacillus Subtilis B1

Khi tình hình bệnh hội chứng tôm chết sớm (EMS) đã gây thiệt hại vô cùng to lớn đối với Nuôi trồng thủy sản thì các giải pháp được đề nghị và áp dụng nhằm hạn chế dịch bệnh. Trong đó, việc tìm hiểu và đưa vi khuẩn có lợi để cạnh tranh và ức chế loài vi khuẩn gây bệnh rất được quan tâm, được cho là giải pháp có nhiều triển vọng phù hợp với điều kiện môi trường, đảm bảo sức khỏe cho con người, cũng như hạn chế được dịch bệnh. Đặc biệt, đưa vi khuẩn Bacillus spp. qua đường tiêu hóa của tôm ngay từ khi mới thả đã hạn chế được mật độ vi khuẩn Vibrio. Nghiên cứu này đã phân lập được các chủng Bacillus subtilis B1, Bacillus subtilis B2, Bacillus amyloliquefaciens B4và thử khả năng đối kháng với vi khuẩn Vibrio parahaemolyticus V1 ở các nồng độ 103, 104, 105, 106 CFU theo dõi ở các thời điểm 6h, 12h, 24h, 48h và 72h. Kết quả cho thấy cả ba chủng vi khuẩn Bacillus trên phân lập được đều có khả năng ức chế tốt vi khuẩn Vibrio parahaemolyticus V1, trong đó vi khuẩn Bacillus amyloliquefaciens B4 làtốt nhất với đường kính vòng kháng khuẩn 52,67 ± 4,31mm ở thời điểm 48h; hai chủng Bacillus subtilis B1, Bacillus subtilis B2 lầnlượt là  49,67 ± 3,15 mm, 44,07 ± 5,19 mm, với mức sai số có ý nghĩa thống kê p < 0,05.

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