Sub-chronic exposure to fipronil induced oxidative stress, biochemical and histopathological changes in the liver and kidney of male albino rats

Objective: This study assessed the effect of chronic exposure to a mixture of heavy metals arsenic (As), cadmium (Cd), and lead (Pb) at a very low environmentally relevant dose along with the effect of coadministration of metallic antioxidants selenium (Se) and zinc (Zn) on hepatic and renal function and oxidative stress parameters in the liver and kidney of female albino rats.Methods: A total of 24 female albino rats were divided into four groups. Animals of the control group received only distilled water. The treated group received mixture of heavy metals As (38.0 ppm), Cd (9.8 ppm), and Pb (22.0 ppm)/kg b.w./day. The supplemented groups received either sodium selenate (10 ppm) or Zn chloride (20 ppm) along with mixture of heavy metals. The treatment period was 90 consecutive days.Results: There was a significant increase in serum glucose, cholesterol, urea and creatinine and decrease in protein and albumin levels in the rats treated with mixture of heavy metals. The activities of serum enzymes, serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase and acid phosphatase and alkaline phosphatase in the liver and kidney of treated animals were also increased. The activities of different oxidative enzymes catalase, superoxide dismutase, glutathione reductase, glutathione peroxidase, and the levels of glutathione reduced significantly and level of malondialdehyde increased in rats treated with metal mixture. Histopathology of liver and kidney tissues exhibited toxic symptoms in treated animals. All the deleterious effects were reversed by cotreatment with either Se or Zn.Conclusion: Both Se and Zn provided protection against oxidative damage and hepatotoxic and nephrotoxic effects produced due to exposure to a mixture of heavy metals As, Cd, and Pb at a very low environmentally relevant dose in female rats for 3 months.

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MODULATORY ROLE OF SELENIUM AND VITAMIN E AGAINST OXIDATIVE STRESS INDUCED HEPATOTOXICITY AND NEPHROTOXICITY IN RATS EXPOSED SUB-CHRONICALLY TO HEXAVALENT CHROMIUM

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2020.v13i8.37900 ◽

2020 ◽

pp. 113-118

Author(s):

SOMA CHOUDHURI ◽

JAHNABI SAHA ◽

SANDEEP DAS ◽

DIPAYAN CHOUDHURI

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Hexavalent Chromium ◽

Chronic Exposure ◽

Male Rats ◽

Sodium Selenate ◽

Albino Rats ◽

Relevant Dose ◽

Liver And Kidney ◽

Serum Glutamate

Objective: The present study assessed the hepatotoxicity and nephrotoxicity associated with oxidative stress induced by chronic exposure to a very low environmentally relevant dose of hexavalent chromium along with the ameliorative potential of selenium and Vitamin E in male rats. Methods: Twenty-four male albino rats were divided into four groups. Animals of control group received only distilled water. The treated group received solution of potassium dichromate (K2Cr2O7) at a dose of 1 mg/kg b.w./day. The third group received sodium selenate (0.25 mg/kg bw) plus Vitamin E (100 mg/kg bw). The supplemented group received sodium selenate plus Vitamin E along with K2Cr2O7 solution. The animals were treated for 90 consecutive days. Results: There was a significant decrease in body weight gain and an increase in liver and kidney weight along with an increase in serum glucose, cholesterol, urea, and creatinine; a decrease in protein and albumin levels in the rats treated with K2Cr2O7. The activities of serum enzymes, serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, acid phosphatase, and alkaline phosphatase, were also increased in treated animals. The activities of enzymes catalase, superoxide dismutase, GPx and the levels of GSH reduced significantly and level of malondialdehyde increased in K2Cr2O7 treated rats. Liver and kidney tissues exhibited features of toxicity in chromium treated animals. All the effects were reversed in supplemented group. Conclusion: Chronic exposure to K2Cr2O7 at a very low environmentally relevant dose caused hepatotoxicity and nephrotoxicity induced by oxidative stress in male albino rats; the effects were ameliorated by supplementation with selenium and Vitamin E in combination.

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Amelorative effect of olive oil against hepatorenal toxicity of cefotaxime in albino rats

International Journal of Pharmacology and Toxicology ◽

10.14419/ijpt.v8i1.31179 ◽

2020 ◽

Vol 8 (1) ◽

pp. 96

Author(s):

Ashraf A. A. Elkomy ◽

Mossad G. E Elsayed ◽

Faten I. El sayed ◽

Ahmed A. Abd el atey

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Olive Oil ◽

Kidney Tissue ◽

Antioxidant Effect ◽

Oxidative Status ◽

Control Group ◽

Albino Rats ◽

Total Protein Level ◽

Liver And Kidney

Due to great hazard effects of antibiotic the following study aimed to investigate the adverse effect of cefotaxime in biochemical, oxidative status and histological examination of Liver and kidney tissue as well as the protective effect of olive oil. Twenty four male Wister albino rats were randomly divided into main four groups including: - G (1): Served as control group and it includes six rats, they were administrated 0.5ml of saline orally for 14 consecutive days. G (2): it includes six rats, they were administered 5ml/kg olive oil orally for 14 consecutive days. G (3): it includes six rats, they were administrated 90mg/kg body weight/twice daily of cefotaxime intramuscular for 14 consecutive days. G (4): it includes six rats, they were administered 5ml/kg olive oil orally concurrently with 90mg/kg body weight/twice daily of cefotaxime. Results revealed that cefotaxime induced significant increases in liver and kidney function parameters including AST, ALT, ALP. creatinine, and urea as well as decrease in albumin and total protein level. Moreover, marked an increase in malondialdehyde (MDA) and decreases in glutathione (GSH) and catalase (CAT) levels. that indicate oxidative stress levels expression in the hepatic and renal tissues following cefotaxime administration. On the beneficial side oral administration of olive oil at the dose 5ml/kg for 14 days significantly mitigate theses toxic effects. So it is concluded that olive oil has great hepatorenal antioxidant effect.

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Triazophos induced oxidative stress and histomorphological changes in liver and kidney of female albino rats

Pesticide Biochemistry and Physiology ◽

10.1016/j.pestbp.2014.03.003 ◽

2014 ◽

Vol 110 ◽

pp. 71-80 ◽

Cited By ~ 24

Author(s):

Dharmender Sharma ◽

Gurinder Kaur Sangha

Keyword(s):

Oxidative Stress ◽

Albino Rats ◽

Liver And Kidney ◽

Histomorphological Changes

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Beneficial effects of quercetin on renal injury and oxidative stress caused by ciprofloxacin in rats

Human & Experimental Toxicology ◽

10.1177/0960327115584686 ◽

2015 ◽

Vol 35 (3) ◽

pp. 276-281 ◽

Cited By ~ 7

Author(s):

H Elbe ◽

Z Dogan ◽

E Taslidere ◽

A Cetin ◽

Y Turkoz

Keyword(s):

Oxidative Stress ◽

Renal Injury ◽

Kidney Tissue ◽

Albino Rats ◽

Therapeutic Effects ◽

Histopathological Changes ◽

Tubular Atrophy ◽

Beneficial Effects ◽

Wistar Albino Rats ◽

And Oxidative Stress

Ciprofloxacin is a broad-spectrum quinolone antibiotic commonly used in clinical practice. Quercetin is an antioxidant belongs to flavonoid group. It inhibits the production of superoxide anion. In this study, we aimed to evaluate the effects of quercetin on renal injury and oxidative stress caused by ciprofloxacin. Twenty-eight female Wistar albino rats were divided into four groups: control, quercetin (20 mg kg−1 day−1 gavage for 21 days), ciprofloxacin (20 mg kg−1 twice a day intraperitoneally for 10 days), and ciprofloxacin + quercetin. Samples were processed for histological and biochemical evaluations. Malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD), and catalase (CAT) activities were measured in kidney tissue. The ciprofloxacin group showed histopathological changes such as infiltration, dilatation in tubules, tubular atrophy, reduction of Bowman’s space, congestion, hemorrhage, and necrosis. In the ciprofloxacin + quercetin group, these histopathological changes markedly reduced. MDA levels increased in the ciprofloxacin group and decreased in the ciptofloxacin + quercetin group. SOD and CAT activities and GSH levels significantly decreased in the ciprofloxacin group. On the other hand, in the ciprofloxacin + quercetin group, SOD and CAT activities and GSH levels significantly increased with regard to the ciprofloxacin group. We concluded that quercetin has antioxidative and therapeutic effects on renal injury and oxidative stress caused by ciprofloxacin in rats.

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Indium Titanium Oxide Nanoparticles Induced Hepatic Damage: Hepatoprotective Role of Novel 2-Imino-4-methyl-1, 2-Dihydropyrimido [5, 4C] Quinoline-5(6H)-one

Advances in Toxicology ◽

10.1155/2014/641813 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Dinesh Bheeman ◽

Sinjula Cheerothsahajan ◽

Sathish Sugumaran ◽

Sankaran Mathan ◽

Ramesh Mathan ◽

...

Keyword(s):

Oxidative Stress ◽

Titanium Oxide ◽

Total Protein ◽

Total Bilirubin ◽

Protective Role ◽

Albino Rats ◽

Oxide Nanoparticles ◽

Histopathological Changes ◽

Titanium Oxide Nanoparticles

Protective role of 2-imino-4-methyl-1, 2-dihydropyrimido [5, 4C] quinoline-5(6H)-one (IMDHPQ) in indium titanium oxide nanoparticles (InTiO NPs) induced hepatotoxicity was analyzed. InTiO NPs were synthesized and given orally to albino rats to assess their hepatotoxicity. NPs mediated oxidative stress and liver tissue pathology were analyzed. Altered antioxidants (GSH, GPx, and catalase) and, biochemical (SGOT, SGPT, ALP, total protein, and total bilirubin) and histopathological changes were observed due to the oxidative stress caused by InTiO NPs. Varying effects of IMDHPQ on each parameter were observed in the present study. The altered parameters of InTiO NPs exposed rats might be due to the oxidative stress caused by NPs and hepatoprotective or ameliorative efficacy of quinoline compound IMDHPQ on signaling and molecular mechanism needs further study.

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Effect of quercetin on parenchymatous organ of the alloxan induced diabetes in male rats

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20204862 ◽

2020 ◽

Vol 8 (11) ◽

pp. 3809

Author(s):

Rizgar Khalid Nabi ◽

Mahdi Ali Abdullah

Keyword(s):

Oxidative Stress ◽

Diabetic Control ◽

Male Rats ◽

Control Group ◽

Albino Rats ◽

Histopathological Changes ◽

Insulin Group ◽

Pancreatic Damage ◽

Parenchymatous Organ ◽

Diabetic Control Group

Background: Diabetes is directly involved in oxidative stress production. Therefore, this work was conducted to investigate the histopathological changes which occur in parenchymatous and to evaluate the antioxidant effect of quercetin in alloxan induced diabetes in male albino rats.Methods: Thirty-six male albino rats were divided into six groups of 6 rats in each group and treated as follows: a control group, quercetin group, diabetic control group, diabetic with quercetin group, diabetic with insulin group, diabetic with quercetin plus insulin group, alloxan was administered as a single dose (140 mg/kg body weight) to induce diabetes.Results: Result showed histopathological changes which included degenerative to necrotic changes of the liver, kidney and pancreas and this are due to the effect of oxidative stress that occurred from diabetes by alloxan. Conversely, quercetin significantly modulated improved histopathological changes founded on this study with or without of insulin, furthermore, results showed that damaged tissues where improved when groups of rats treated with quercetin and insulin together.Conclusions: It has been concluded that the quercetin could be promising antioxidants for reducing the risk of oxidation induced by diabetes that lead to nephrotoxicity, hepatotoxicity and pancreatic damage.

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The Protective Effect of Naringenin-Oxime on Cisplatin-Induced Toxicity in Rats

Biochemistry Research International ◽

10.1155/2017/9478958 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 18

Author(s):

Ismail Koyuncu ◽

Abdurrahim Kocyigit ◽

Ataman Gonel ◽

Erkan Arslan ◽

Mustafa Durgun

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Comet Assay ◽

Protective Effect ◽

Stress Index ◽

Mononuclear Leukocytes ◽

Albino Rats ◽

Peripheral Blood Mononuclear ◽

Liver And Kidney ◽

And Oxidative Stress

The aim of this study is to examine the protective effect of naringenin-oxime (NOX) on cisplatin-induced major organ toxicity and DNA damage in rats. Thirty-five male Wistar albino rats were equally split into five groups as follows: control (i.p., 0.1 ml of saline), Cis administration (i.p., 7 mg/kg b.w.), NOX treatment (i.p., 20 mg/kg b.w., daily for ten days), Cis + NOX20, and Cis + NOX40 combination (i.p., 20 and 40 mg/kg b.w., daily for ten days). Serum and peripheral blood mononuclear leukocytes (PBMC) were obtained from blood. Malondialdehyde, glutathione, total antioxidant and oxidant status, and catalase were measured in serum, liver, and kidney, and oxidative stress index was calculated. In parallel, paraoxonase and arylesterase activities were tested in liver and serum. We used 8-OHdOG as a marker for DNA damage in serum via ELISA and in PMBC via comet assay. Treatment with Cis elevated the levels of serum biochemical parameters, oxidative stress, and DNA damage. Pretreatments of NOX restored biochemical and oxidative stress parameters in serum, renal, and liver tissues (p<0.01) and reduced 8-OHdG level, a finding further supported by comet assay in PBMC. Observations of the present study support the fact that treatment with NOX prevents Cis-induced hepatotoxicity, nephrotoxicity, and genotoxicity by restoring antioxidant system.

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In Vivo Biocompatibility Studies of Nano TiO2 Materials

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.79-82.389 ◽

2009 ◽

Vol 79-82 ◽

pp. 389-392 ◽

Cited By ~ 15

Author(s):

Wei Han ◽

Yue Dan Wang ◽

Yu Feng Zheng

Keyword(s):

Oxidative Stress ◽

Animal Model ◽

Catalase Activity ◽

Histopathological Changes ◽

Vacuolar Degeneration ◽

Tio2 Nanomaterials ◽

Liver And Kidney ◽

Different Dimensions ◽

And Oxidative Stress

TiO2 nanomaterials with different dimensions(zero and one), sizes(20nm, 50nm and 100nm in diameter) and crystal structures(100% rutile, 100% anatase and combination of 20% rutile and 80% anatase) were confected to suspensions and ointment with varied concentrations and evaluated in animal model (Balb-c mouse). These mouse were divided into various groups randomly, with suspension intraperitoneally injected or ointment transdermally daubed. Heart, lung, liver and kidney were collected and prepared to HE sample after one week. Spectrophotometry was applied to study total antioxide capability and catalase activity of blood and tissues. It has been shown that all TiO2 nanomaterial groups had no effect on lives’ morphology and oxidative stress, with no obvious histopathological changes observed in heart, lung, liver and kidney, and these tissues presented no vacuolar degeneration, necrosis edema, engorgement and inflammation.

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