scholarly journals The impact of carbon source on cell growth and the production of bioactive compounds in cell suspensions of Hancornia speciosa Gomes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Luciana Arantes Dantas ◽  
Paula Sperotto Alberto Faria ◽  
Bruno Matheus Mendes Dário ◽  
Ana Luíza Martins Arantes ◽  
Fabiano Guimarães Silva ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Cell Growth ◽  
Water Absorption ◽  
Bioactive Compounds ◽  
Osmotic Potential ◽  
Carbon Sources ◽  
Biomass Accumulation ◽  
Dry Cell Weight ◽  
Hancornia Speciosa ◽  
The Impact

AbstractBelonging to the Brazilian flora, the species Hancornia speciosa (Gomes), known as mangabeira, has bioactive compounds of interest, such as flavonoids, xanthones, and proanthocyanidins. The objective of this study was to determine how the supplementation of sugars in culture medium affects the osmotic potential of the medium, as well as its influence on cell growth and on the concentration of phenolic compounds. For this purpose, after 90 days of subculture, 20 mL aliquots of the cultures were added to flasks containing 20 mL of medium with different sugars (glucose, fructose, sucrose, mannitol, and sorbitol) under a 16-h photoperiod with a spectral range between 400 and 700 nm of photosynthetically active radiation (45–55 μmol m−2 s−1) in a shaker at 110 rpm. After 30 days, the pH, electrical conductivity, osmotic potential, biomass accumulation, and concentrations of phenolic compounds were evaluated. Regardless of their concentration in the medium, the sugars sorbitol and mannitol provided more unfavorable conditions for water absorption at the cellular level, reducing the water potential of the medium. Sucrose favored greater water absorption and biomass accumulation. Among the various sugar concentrations, 3% (30 g/L) sucrose or glucose improved the accumulation of fresh and dry cell weight and the production of polyphenols such as chlorogenic acid, epicatechin, rosmarinic acid, hesperidin, rutin, and quercetin. In addition, they resulted in a higher osmotic potential of the medium and larger cells than other carbon sources. Despite the differences in cell size, no culture conditions compromised cell survival.

scholarly journals The Impact of Carbon Source on Cell Growth and the Production of Bioactive Compounds in Cell Suspensions of Hancornia speciosa Gomes

2021 ◽  
Author(s):  
Luciana Arantes Dantas ◽  
Paula Sperotto Alberto Faria ◽  
Bruno Matheus Mendes Dário ◽  
Ana Luíza Martins Arantes ◽  
Fabiano Guimarães Silva ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Cell Growth ◽  
Water Absorption ◽  
Bioactive Compounds ◽  
Osmotic Potential ◽  
Carbon Sources ◽  
Biomass Accumulation ◽  
Dry Cell Weight ◽  
Hancornia Speciosa ◽  
The Impact

Abstract Belonging to the Brazilian flora, the species Hancornia speciosa (Gomes), known as mangabeira, has bioactive compounds of interest, such as flavonoids, xanthones, and proanthocyanidins. The objective of this study was to determine how the supplementation of sugars in culture medium affects the osmotic potential of the medium, as well as its influence on cell growth and on the concentration of phenolic compounds. For this purpose, after 90 days of subculture, 20 ml aliquots of the cultures were added to flasks containing 20 ml of medium with different sugars (glucose, fructose, sucrose, mannitol, and sorbitol) under a 16-h photoperiod with a spectral range between 400 and 700 nm of photosynthetically active radiation (45-55 μmol m -2 s -1 ) in a shaker at 110 rpm. After 30 days, the pH, electrical conductivity, osmotic potential, biomass accumulation, and concentrations of phenolic compounds were evaluated. Regardless of their concentration in the medium, the sugars sorbitol and mannitol provided more unfavorable conditions for water absorption at the cellular level, reducing the water potential of the medium. Sucrose favored greater water absorption and biomass accumulation. Among the various sugar concentrations, 3% (30 g/L) sucrose or glucose improved the accumulation of fresh and dry cell weight and the production of polyphenols such as chlorogenic acid, epicatechin, rosmarinic acid, hesperidin, rutin, and quercetin. In addition, they resulted in a higher osmotic potential of the medium and larger cells than other carbon sources. Despite the differences in cell size, no culture conditions compromised cell survival.

scholarly journals In Vitro Gastrointestinal Digestion Impact on the Bioaccessibility and Antioxidant Capacity of Bioactive Compounds from Tomato Flours Obtained after Conventional and Ohmic Heating Extraction

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 554
Author(s):  
Marta C. Coelho ◽  
Tânia B. Ribeiro ◽  
Carla Oliveira ◽  
Patricia Batista ◽  
Pedro Castro ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Solid Fraction ◽  
Ohmic Heating ◽  
Coumaric Acid ◽  
Insoluble Dietary Fiber ◽  
The Impact

In times of pandemic and when sustainability is in vogue, the use of byproducts, such as fiber-rich tomato byproducts, can be an asset. There are still no studies on the impact of extraction methodologies and the gastrointestinal tract action on bioactive properties. Thus, this study used a solid fraction obtained after the conventional method (SFCONV) and a solid fraction after the ohmic method (SFOH) to analyze the effect of the gastrointestinal tract on bioactive compounds (BC) and bioactivities. Results showed that the SFOH presents higher total fiber than SFCONV samples, 62.47 ± 1.24–59.06 ± 0.67 g/100 g DW, respectively. Both flours present high amounts of resistant protein, representing between 11 and 16% of insoluble dietary fiber. Furthermore, concerning the total and bound phenolic compounds, the related antioxidant activity measured by 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical cation decolorization assay presented significantly higher values for SFCONV than SFOH samples (p < 0.05). The main phenolic compounds identified in the two flours were gallic acid, rutin, and p-coumaric acid, and carotenoids were lycopene, phytofluene, and lutein, all known as health promoters. Despite the higher initial values of SFCONV polyphenols and carotenoids, these BCs’ OH flours were more bioaccessible and presented more antioxidant capacity than SFCONV flours, throughout the simulated gastrointestinal tract. These results confirm the potential of ohmic heating to modify the bioaccessibility of tomato BC, enhancing their concentrations and improving their antioxidant capacity.

scholarly journals Environmental Impact on Seaweed Phenolic Production and Activity: An Important Step for Compound Exploitation

Marine Drugs ◽  
2021 ◽  
Vol 19 (5) ◽  
pp. 245
Author(s):  
Silvia Lomartire ◽  
João Cotas ◽  
Diana Pacheco ◽  
João Carlos Marques ◽  
Leonel Pereira ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Animal Health ◽  
Antimicrobial Activities ◽  
Pharmaceutical Products ◽  
Human Beings ◽  
Healthy Foods ◽  
Beneficial Effects ◽  
Natural Drugs ◽  
The Impact

Seaweeds are a potential source of bioactive compounds that are useful for biotechnological applications and can be employed in different industrial areas in order to replace synthetic compounds with components of natural origin. Diverse studies demonstrate that there is a solid ground for the exploitation of seaweed bioactive compounds in order to prevent illness and to ensure a better and healthier lifestyle. Among the bioactive algal molecules, phenolic compounds are produced as secondary metabolites with beneficial effects on plants, and also on human beings and animals, due to their inherent bioactive properties, which exert antioxidant, antiviral, and antimicrobial activities. The use of phenolic compounds in pharmaceutical, nutraceutical, cosmetics, and food industries may provide outcomes that could enhance human health. Through the production of healthy foods and natural drugs, bioactive compounds from seaweeds can help with the treatment of human diseases. This review aims to highlight the importance of phenolic compounds from seaweeds, the scope of their production in nature and the impact that these compounds can have on human and animal health through nutraceutical and pharmaceutical products.

scholarly journals Impact of Different Extraction Solvents on Bioactive Compounds and Antioxidant Capacity from the Root of Salacia chinensis L.

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Thanh Van Ngo ◽  
Christopher James Scarlett ◽  
Michael Christian Bowyer ◽  
Phuong Duc Ngo ◽  
Quan Van Vuong
Keyword(s):  
Phenolic Compounds ◽  
Secondary Metabolites ◽  
Antioxidant Capacity ◽  
Organic Solvents ◽  
Bioactive Compounds ◽  
Total Solid ◽  
Extraction Solvent ◽  
Phytochemical Composition ◽  
Extraction Solvents ◽  
The Impact

This study aimed to study the impact of selected common organic solvents on extractable solids, phytochemical composition, and antioxidant capacity of S. chinensis. The results showed that the tested solvents played an important role in extraction of total solid and phytochemical composition as well as antioxidant capacity of S. chinensis. Acetone (50% v/v) was found to be the optimal extraction solvent for extractable solids (12.2%), phenolic compounds (60 mg GAE/g DW), flavonoids (100 mg CE/g DW), proanthocyanidins (47.4 mg CE/g DW), and saponins (754 mg EE/g DW) as well as antioxidant capacity (ABTS 334 mM TE/g DW, DPPH 470 mM TE/g DW, FRAP 347 mM TE/g DW, and CUPRAC 310 mM TE/g DW). The extract prepared from 50% acetone had high levels of bioactive compounds (TPC 555 mg GAE/g CRE, flavonoids 819 mg CE/g CRE, proanthocyanidins 392 mg CE/g CRE, and saponins 1,880 mg EE/g CRE) as well as antioxidant capacity (ABTS 414 mM TE/g, DPPH 407 mM TE/g, FRAP 320 mg TE/g, and CUPRAC 623 mM TE/g), thus further confirming that 50% acetone is the solvent of choice. Therefore, 50% acetone is recommended for extraction of phenolic compounds, their secondary metabolites, saponins, and antioxidant capacity from the root of S. chinensis for further isolation and utilisation.

scholarly journals Location effects across northeastern Europe on bioactive compounds in apple fruit

2019 ◽  
Vol 28 (2) ◽  
Author(s):  
Jonas Viškelis ◽  
Nobertas Uselis ◽  
Mindaugas Liaudanskas ◽  
Juozas Lanauskas ◽  
Pawel Bielicki ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Apple Tree ◽  
Total Content ◽  
Total Phenolic ◽  
The South ◽  
Climate Conditions ◽  
North Direction ◽  
The Impact ◽  
Apple Fruits

A multi-location trial was performed in apple orchards planted in 2005 under a uniform scheme in Poland, Lithuania, Latvia and Estonia. The aim of this study was to evaluate the impact of the geographical apple-tree growing location on the accumulation of dominant bioactive compounds in apple fruits: phenolics and triterpenes as the most valuable substances for human health. The study included two apple cultivars, ‘Auksis’ and ‘Ligol’, on B.396 rootstock and was carried out from 2016 to 2018. Geographical apple-tree growing location had a significant impact on the composition and total content of the tested bioactive compounds in apple fruits. Increased accumulation of phenolic and triterpene compounds was recorded in the south–north direction. This could be explained by the different climate conditions in the trial locations: the sum of active temperatures gradually decreased by 200–300 °C in the south–north direction, and the vegetation period becomes shorter. Apples grown in Estonia, depending on the tested cultivar, had 77–139% more total phenolic compounds. Significant differences were recorded for all groups of phenolic compounds. The differences between the trial sites in accumulation of triterpene compounds were lower, although apples in at farthest north location had 18–32% more total triterpene compounds than apples grown in south.

scholarly journals Bioactive compounds and antioxidant activity in tomatoes (Lycopersicon esculentum L.) cultivars in natura and after thermal processing

2020 ◽  
Vol 9 (11) ◽  
pp. e44991110192
Author(s):  
Angela Vacaro de Souza ◽  
Jéssica Marques de Mello ◽  
Vitória Ferreira da Silva Fávaro ◽  
Victória Farias da Silva ◽  
Diogo de Lucca Sartori ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Lycopersicon Esculentum ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Thermal Processing ◽  
Antioxidant Activities ◽  
The Impact ◽  
Β Carotene

The aim of the study was to evaluate the impact of processing by cooking in natura fruits on the content of bioactive compounds - vitamin C and ascorbic acid, phenolic compounds, flavonoids, carotenoids and anthocyanins, lycopene and β-carotene - and on antioxidant activity - DPPH and FRAP - of 9 varieties of commercial and non-commercial tomatoes. The fruits were harvested when they reached the point of physiological maturation, selected and evaluated in natura, and after grinding and cooking for 30 minutes. At the end of the experiment it was found that all bioactive compounds analyzed showed quantitative reductions when the fruits were processed, with cherry cultivars - 7, 8 and 9 - those that showed superior results compared to different bioactive compounds evaluated, proving to be interesting to be better explored. Regarding the antioxidant activities, the processed fruits of these cultivars showed less losses showing potential to be submitted to processing.

scholarly journals Reduction of antinutrients and maintenance of bioactive compounds in flour from agro-industrial residue of acerola (Malpighia emarginata D.C.)

2020 ◽  
Vol 9 (9) ◽  
pp. e980998054
Author(s):  
Viviane Martins Barros ◽  
Jane de Jesus da Silveira Moreira ◽  
Maria Terezinha Santos Leite Neta ◽  
Tatiana Pacheco Nunes ◽  
Evelyn Horanyi Silva Costa Vasvary ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Volatile Compounds ◽  
Bioactive Compounds ◽  
Physicochemical Characterization ◽  
Significant Loss ◽  
Alternative Source ◽  
Malpighia Emarginata ◽  
Industrial Residue ◽  
Drying Conditions ◽  
The Impact

This study was aimed at analyzing the reduction of antinutrients, preserving apparent phenolic compounds in acerola flour residue by employing drying techniques. The optimal drying conditions were then determined in the wet residue, analyzing antinutrient concentration and apparent phenolic compounds in the residue and subsequently in the acerola flour. The physicochemical characterization of the flour was carried out, determining its antioxidant activity, thereby assessing the impact of the drying process on the composition of volatile compounds. The optimal drying conditions were found for a temperature of 65ºC for 120 min, reducing water activity by 0.3 and moisture content by 13.89%, which are considered adequate conditions for flours. Carbohydrate content represented 68.72 g/100g, while proteins represented 12.55 g/100g of the centesimal composition of the flour. Significant reductions (p<0.5) of antinutrients were observed in saponins (35.9%), followed by phytates (32.8%) and condensed tannins (11.52%). Losses of 18.7% of apparent phenolic compounds were also observed. For volatile compounds, a significant loss of esters with a significant increase in alcohol content was noticed. Thermal processing reduced aroma complexity, but maintained important bioactive compounds, such as linalool and caryophyllene. In this regard, taking into account the results of the present study, the agro-industrial residue of acerola proved to be an alternative source of antioxidants with a reduced antinutritional impact and may be incorporated as an ingredient in the formulation of new food products. Moreover, this agro-industrial residue reduces the environmental impact caused by the fruit pulp processing industry.

The Impact of Plant Bioactive Compounds on Aging and Fertility of Diverse Organisms: A Review

2020 ◽  
Vol 20 (13) ◽  
pp. 1287-1299
Author(s):  
Muhammad Akram Mohd Noordin ◽  
Mahanem Mat Noor ◽  
Wan Mohd Aizat
Keyword(s):  
Clinical Trial ◽  
Bioactive Compounds ◽  
Defense Mechanisms ◽  
Healthy Aging ◽  
Antioxidant Defense ◽  
Senior Citizens ◽  
Aging Population ◽  
Human Clinical Trial ◽  
Fertility Reduction ◽  
The Impact

It is expected that in 2050, there will be more than 20% of senior citizens aged over 60 years worldwide. Such alarming statistics require immediate attention to improve the health of the aging population. Since aging is closely related to the loss of antioxidant defense mechanisms, this situation eventually leads to numerous health problems, including fertility reduction. Furthermore, plant extracts have been used in traditional medicine as potent antioxidant sources. Although many experiments had reported the impact of various bioactive compounds on aging or fertility, there is a lack of review papers that combine both subjects. In this review, we have collected and discussed various bioactive compounds from 26 different plant species known to affect both longevity and fertility. These compounds, including phenolics and terpenes, are mostly involved in the antioxidant defense mechanisms of diverse organisms such as rats, mites, fruit flies, roundworms, and even roosters. A human clinical trial should be considered in the future to measure the effects of these bioactive compounds on human health and longevity. Ultimately, these plant-derived compounds could be developed into health supplements or potential medical drugs to ensure a healthy aging population.

Advances in the Chromatographic Separation and Determination of Bioactive Compounds for Assessing the Nutrient Profile of Nuts

2020 ◽  
Vol 16 ◽  
Author(s):  
Natasa P. Kalogiouri ◽  
Natalia Manousi ◽  
Erwin Rosenberg ◽  
George A. Zachariadis ◽  
Victoria F. Samanidou
Keyword(s):  
Fatty Acids ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
High Performance ◽  
Unsaturated Fatty Acids ◽  
Nutritional Deficiencies ◽  
Analytical Technique ◽  
High Volatility ◽  
Analytical Approaches

Background:: Nuts have been incorporated into guidelines for healthy eating since they contain considerable amounts of antioxidants and their effects are related to health benefits since they contribute to the prevention of nutritional deficiencies. The micronutrient characterization is based mainly on the determination of phenolics which is the most abundant class of bioactive compounds in nuts. Terpenes constitute another class of bioactive compounds that are present in nuts and show high volatility. The analysis of phenolic compounds and terpenes are very demanding tasks that require optimization of the chromatographic conditions to improve the separation of the components. Moreover, nuts are rich in unsaturated fatty acids and they are therefore considered as cardioprotective. Gas chromatography is the predominant instrumental analytical technique for the determination of derivatized fatty acids and terpenes in food matrices, while high performance liquid chromatography is currently the most popular technique for the determination of phenolic compounds Objective:: This review summarizes all the recent advances in the optimization of the chromatographic conditions for the determination of phenolic compounds, fatty acids and terpenes in nuts Conclusion:: The state-of-the art in the technology available is critically discussed, exploring new analytical approaches to reduce the time of analysis and improve the performance of the chromatographic systems in terms of precision, reproducibility, limits of detection and quantification and overall quality of the results

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