scholarly journals The rare sugar d-tagatose protects plants from downy mildews and is a safe fungicidal agrochemical

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Susumu Mochizuki ◽  
Takeshi Fukumoto ◽  
Toshiaki Ohara ◽  
Kouhei Ohtani ◽  
Akihide Yoshihara ◽  
...  
Keyword(s):  
Plant Diseases ◽  
Structural Development ◽  
Phosphomannose Isomerase ◽  
Inhibitory Effects ◽  
Food Ingredient ◽  
Rare Sugar ◽  
Initial Infection ◽  
Downy Mildews ◽  
Wide Range ◽  
Mannose Metabolism

AbstractThe rare sugar d-tagatose is a safe natural product used as a commercial food ingredient. Here, we show that d-tagatose controls a wide range of plant diseases and focus on downy mildews to analyze its mode of action. It likely acts directly on the pathogen, rather than as a plant defense activator. Synthesis of mannan and related products of d-mannose metabolism are essential for development of fungi and oomycetes; d-tagatose inhibits the first step of mannose metabolism, the phosphorylation of d-fructose to d-fructose 6-phosphate by fructokinase, and also produces d-tagatose 6-phosphate. d-Tagatose 6-phosphate sequentially inhibits phosphomannose isomerase, causing a reduction in d-glucose 6-phosphate and d-fructose 6-phosphate, common substrates for glycolysis, and in d-mannose 6-phosphate, needed to synthesize mannan and related products. These chain-inhibitory effects on metabolic steps are significant enough to block initial infection and structural development needed for reproduction such as conidiophore and conidiospore formation of downy mildew.

scholarly journals Microbial antagonists against plant pathogens in Iran: A review

2020 ◽  
Vol 5 (1) ◽  
pp. 404-440 ◽  
Author(s):  
Mehrdad Alizadeh ◽  
Yalda Vasebi ◽  
Naser Safaie
Keyword(s):  
Biological Control ◽  
Chemical Control ◽  
Plant Disease ◽  
Plant Pathogens ◽  
Plant Diseases ◽  
Agricultural Products ◽  
Control Measures ◽  
Wide Range ◽  
Plant Disease Management ◽  
Published Research

AbstractThe purpose of this article was to give a comprehensive review of the published research works on biological control of different fungal, bacterial, and nematode plant diseases in Iran from 1992 to 2018. Plant pathogens cause economical loss in many agricultural products in Iran. In an attempt to prevent these serious losses, chemical control measures have usually been applied to reduce diseases in farms, gardens, and greenhouses. In recent decades, using the biological control against plant diseases has been considered as a beneficial and alternative method to chemical control due to its potential in integrated plant disease management as well as the increasing yield in an eco-friendly manner. Based on the reported studies, various species of Trichoderma, Pseudomonas, and Bacillus were the most common biocontrol agents with the ability to control the wide range of plant pathogens in Iran from lab to the greenhouse and field conditions.

scholarly journals Trends in Molecular Diagnosis and Diversity Studies for Phytosanitary Regulated Xanthomonas

2021 ◽  
Vol 9 (4) ◽  
pp. 862
Author(s):  
Vittoria Catara ◽  
Jaime Cubero ◽  
Joël F. Pothier ◽  
Eran Bosis ◽  
Claude Bragard ◽  
...  
Keyword(s):  
Molecular Diagnosis ◽  
Bacterial Species ◽  
Management Strategies ◽  
Diagnostic Methods ◽  
Plant Diseases ◽  
Wild Plants ◽  
Future Climate Change ◽  
Climate Change Scenarios ◽  
Wide Range ◽  
Diversity Studies

Bacteria in the genus Xanthomonas infect a wide range of crops and wild plants, with most species responsible for plant diseases that have a global economic and environmental impact on the seed, plant, and food trade. Infections by Xanthomonas spp. cause a wide variety of non-specific symptoms, making their identification difficult. The coexistence of phylogenetically close strains, but drastically different in their phenotype, poses an added challenge to diagnosis. Data on future climate change scenarios predict an increase in the severity of epidemics and a geographical expansion of pathogens, increasing pressure on plant health services. In this context, the effectiveness of integrated disease management strategies strongly depends on the availability of rapid, sensitive, and specific diagnostic methods. The accumulation of genomic information in recent years has facilitated the identification of new DNA markers, a cornerstone for the development of more sensitive and specific methods. Nevertheless, the challenges that the taxonomic complexity of this genus represents in terms of diagnosis together with the fact that within the same bacterial species, groups of strains may interact with distinct host species demonstrate that there is still a long way to go. In this review, we describe and discuss the current molecular-based methods for the diagnosis and detection of regulated Xanthomonas, taxonomic and diversity studies in Xanthomonas and genomic approaches for molecular diagnosis.

scholarly journals A High-Quality Grapevine Downy Mildew Genome Assembly Reveals Rapidly Evolving and Lineage-Specific Putative Host Adaptation Genes

2019 ◽  
Vol 11 (3) ◽  
pp. 954-969 ◽  
Author(s):  
Yann Dussert ◽  
Isabelle D Mazet ◽  
Carole Couture ◽  
Jérôme Gouzy ◽  
Marie-Christine Piron ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Genome Assembly ◽  
Population Genomics ◽  
Plasmopara Viticola ◽  
Plant Diseases ◽  
Host Specialization ◽  
Grapevine Downy Mildew ◽  
Downy Mildews ◽  
Genes Encoding ◽  
High Level

Abstract Downy mildews are obligate biotrophic oomycete pathogens that cause devastating plant diseases on economically important crops. Plasmopara viticola is the causal agent of grapevine downy mildew, a major disease in vineyards worldwide. We sequenced the genome of Pl. viticola with PacBio long reads and obtained a new 92.94 Mb assembly with high contiguity (359 scaffolds for a N50 of 706.5 kb) due to a better resolution of repeat regions. This assembly presented a high level of gene completeness, recovering 1,592 genes encoding secreted proteins involved in plant–pathogen interactions. Plasmopara viticola had a two-speed genome architecture, with secreted protein-encoding genes preferentially located in gene-sparse, repeat-rich regions and evolving rapidly, as indicated by pairwise dN/dS values. We also used short reads to assemble the genome of Plasmopara muralis, a closely related species infecting grape ivy (Parthenocissus tricuspidata). The lineage-specific proteins identified by comparative genomics analysis included a large proportion of RxLR cytoplasmic effectors and, more generally, genes with high dN/dS values. We identified 270 candidate genes under positive selection, including several genes encoding transporters and components of the RNA machinery potentially involved in host specialization. Finally, the Pl. viticola genome assembly generated here will allow the development of robust population genomics approaches for investigating the mechanisms involved in adaptation to biotic and abiotic selective pressures in this species.

Inhibitory effects of catecholamines in canine cardiac Purkinje fibers

1976 ◽  
Vol 231 (5) ◽  
pp. 1415-1420 ◽  
Author(s):  
P Posner ◽  
EL Farrar ◽  
CR Lambert
Keyword(s):  
Negative Response ◽  
Chronotropic Effect ◽  
Inhibitory Effects ◽  
Purkinje Fibers ◽  
Spontaneous Rate ◽  
Negative Chronotropic Effect ◽  
Cardiac Purkinje Fibers ◽  
Wide Range ◽  
Low Levels

The effect of catecholamines over a wide range of concentrations was studied on 42K uptake and efflux, as well as on spontaneous rate in canine cardiac Purkinje fibers. Low levels of catecholamines (less than 10(-10) M epinephrine; less than 10(-9) M norepinephrine) decreased automaticity. This negative chronotropic effect was blocked by phentolamine and mimicked by phenylephrine. These low levels of epinephrine and norepinephrine also inhibited 42K uptake by Purkinje fibers but had no effect on 42K efflux. The inhibition of 42K uptake was blocked by phentolamine and verapamil and mimicked by phenylephrine. The data indicate an alpha-receptor-mediated negative response of rate and 42K uptake to low levels of catecholamine. The end result is discussed in terms of a competitive increase in the influx of Ca2+ rather than Na+ and an indirect inhibition of the Na+-K+ pump.

scholarly journals STRUCTURAL DEVELOPMENT AND RESEARCH OF TECHNOLOGICAL PARAMETERS OF MACHINES FOR CENTRIFUGAL -PLANETARY VIBRATION TREATMENT

2020 ◽  
pp. 41-50
Author(s):  
Leonid Yaroshenko ◽  
Igor Kupchuk ◽  
Mykhailo Zamrii
Keyword(s):  
High Efficiency ◽  
Metal Removal ◽  
Three Dimensional ◽  
High Hardness ◽  
Working Environment ◽  
Repeated Loading ◽  
Structural Development ◽  
Technological Parameters ◽  
Advantages And Disadvantages ◽  
Wide Range

The paper analyzes current state and prospects of further development of technology and equipment for mechanization and automation of finishing and cleaning of details. It is stated that the most effective for this purpose are the methods of abrasive machining, which include bulk galvanizing, vibration, centrifugal-rotary and centrifugal-planetary processing. These methods reduce the complexity and cost of processing, which in some cases reaches 20% of the total cost of manufacturing parts. Each of these methods has a different level of efficiency, certain advantages and disadvantages, the level of versatility and scope of effective application. The processes of vibration processing are quite deeply studied, for its industrial mass-produced technological equipment, but they have certain shortcomings that limit their use for further widespread implementation. Centrifugal-rotary processing is a more productive process of three-dimensional finishing and cleaning treatment, but its scope is limited by the possibility of processing parts that are not complex, usually flat. The most productive methods of finishing and cleaning of details include centrifugal-planetary volume processing which high efficiency is caused by repeated loading of particles of working load by inertial forces that creates preconditions for the solutions of a wide range of technological problems, for example, processing of details of difficult form, small weight and the sizes from materials of high hardness or viscosity that represents a serious problem for other methods of volume processing. The constructive scheme is offered in the work and the influence of the composition of the abrasive free-granular working environment on the productivity of the machine for centrifugal-planetary processing of details is investigated. The kinetics of metal removal from the surface of machined parts using different types of abrasive working environment is analyzed. The results of comparative machining of parts in a torus vibrating machine and a machine for centrifugal-planetary machining are given. It is shown that the simultaneous use of centrifugal-planetary and vibration processing methods allows to increase the intensity of the process while ensuring high quality machining of parts of relatively complex shape. The constructive scheme of the machine which allows to implement the specified combined method of processing is offered and described.

scholarly journals Bacterial exposure leads to variable mortality but not a measurable increase in surface antimicrobials across ant species

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10412
Author(s):  
Omar Halawani ◽  
Robert R. Dunn ◽  
Amy M. Grunden ◽  
Adrian A. Smith
Keyword(s):  
Staphylococcus Epidermidis ◽  
Solenopsis Invicta ◽  
Antimicrobial Compounds ◽  
Inhibitory Effects ◽  
E Coli ◽  
Aphaenogaster Rudis ◽  
Wide Range ◽  
Health Threats ◽  
Environmental Bacteria ◽  
Significant Mortality

Social insects have co-existed with microbial species for millions of years and have evolved a diversity of collective defenses, including the use of antimicrobials. While many studies have revealed strategies that ants use against microbial entomopathogens, and several have shown ant-produced compounds inhibit environmental bacterial growth, few studies have tested whether exposure to environmental bacteria represents a health threat to ants. We compare four ant species’ responses to exposure to Escherichia coli and Staphylococcus epidermidis bacteria in order to broaden our understanding of microbial health-threats to ants and their ability to defend against them. In a first experiment, we measure worker mortality of Solenopsis invicta, Brachymyrmex chinensis, Aphaenogaster rudis, and Dorymyrmex bureni in response to exposure to E. coli and S. epidermidis. We found that exposure to E. coli was lethal for S. invicta and D. bureni, while all other effects of exposure were not different from experimental controls. In a second experiment, we compared the antimicrobial ability of surface extracts from bacteria-exposed and non-exposed S. invicta and B. chinensis worker ants, to see if exposure to E. coli or S. epidermidis led to an increase in antimicrobial compounds. We found no difference in the inhibitory effects from either treatment group in either species. Our results demonstrate the susceptibility to bacteria is varied across ant species. This variation may correlate with an ant species’ use of surface antimicrobials, as we found significant mortality effects in species which also were producing antimicrobials. Further exploration of a wide range of both bacteria and ant species is likely to reveal unique and nuanced antimicrobial strategies and deepen our understanding of how ant societies respond to microbial health threats.

scholarly journals Introduction to the Special Issue: Legumes as Food Ingredient: Characterization, Processing, and Applications

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1525
Author(s):  
Alfonso Clemente ◽  
Jose C. Jimenez-Lopez
Keyword(s):  
Grain Legumes ◽  
Sensory Profile ◽  
Special Issue ◽  
Food Ingredient ◽  
Food Ingredients ◽  
Wide Range ◽  
Key Aspects ◽  
Lactic Bacteria ◽  
Essential Food

Legumes are major ingredients in the Mediterranean diet, playing an essential role in developing countries. Grain legumes, such as lentil, chickpea, pea, lupin and beans, among others, are recognized as good sources of proteins, starch, fiber, vitamins and minerals for human nutrition, being an essential food crop for people worldwide. Due to their nutritional and techno-functional properties, legumes are widely used by the food industry as ingredients in a wide range of products for general and specific groups of the population, including vegetarians, diabetics or celiac patients. The Special Issue “Legumes as Food Ingredients: Characterization, Processing, and Applications” covers key aspects regarding the nutritional quality of legume flours and their derived products, as well as the health benefits of some of their bioactive components. The amounts of antinutritional components, such as certain allergens that might pose risks to sensitized consumers, are reported to be reduced by processing. Several pretreatments, including fermentation with lactic bacteria and yeasts, are used to improve the nutritional and sensory profile of the legume-derived products, increasing their acceptance by consumers.

scholarly journals Lipopeptides as the Antifungal and Antibacterial Agents: Applications in Food Safety and Therapeutics

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Khem Raj Meena ◽  
Shamsher S. Kanwar
Keyword(s):  
Antimicrobial Agents ◽  
Antibacterial Activities ◽  
Plant Diseases ◽  
Biologically Active ◽  
Economic Losses ◽  
Biological Origin ◽  
Global Food Security ◽  
Wide Range ◽  
Potential Inhibitors ◽  
Global Food

A lot of crops are destroyed by the phytopathogens such as fungi, bacteria, and yeast leading to economic losses to the farmers. Members of theBacillusgenus are considered as the factories for the production of biologically active molecules that are potential inhibitors of growth of phytopathogens. Plant diseases constitute an emerging threat to global food security. Many of the currently available antimicrobial agents for agriculture are highly toxic and nonbiodegradable and thus cause extended environmental pollution. Moreover, an increasing number of phytopathogens have developed resistance to antimicrobial agents. The lipopeptides have been tried as potent versatile weapons to deal with a variety of phytopathogens. All the three families ofBacilluslipopeptides, namely, Surfactins, Iturins and Fengycins, have been explored for their antagonistic activities towards a wide range of phytopathogens including bacteria, fungi, and oomycetes. Iturin and Fengycin have antifungal activities, while Surfactin has broad range of potent antibacterial activities and this has also been used as larvicidal agent. Interestingly, lipopeptides being the molecules of biological origin are environmentally acceptable.

scholarly journals Transcriptomic Analysis of Orange Fruit Treated with Pomegranate Peel Extract (PGE)

Plants ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 101 ◽  
Author(s):  
Imen Belgacem ◽  
Sonia Pangallo ◽  
Ahmed Abdelfattah ◽  
Flora V. Romeo ◽  
Santa O. Cacciola ◽  
...  
Keyword(s):  
Gene Ontology ◽  
Plant Diseases ◽  
Pathway Enrichment Analysis ◽  
Plant Host ◽  
Pomegranate Peel ◽  
Transcriptomic Response ◽  
Wide Range ◽  
Orange Fruit ◽  
Pomegranate Peel Extract ◽  
Peel Extract

A Pomegranate Peel Extract (PGE) has been proposed as a natural antifungal substance with a wide range of activity against plant diseases. Previous studies showed that the extract has a direct antimicrobial activity and can elicit resistance responses in plant host tissues. In the present study, the transcriptomic response of orange fruit toward PGE treatments was evaluated. RNA-seq analyses, conducted on wounded fruits 0, 6, and 24 h after PGE applications, showed a significantly different transcriptome in treated oranges as compared to control samples. The majority (273) of the deferentially expressed genes (DEGs) were highly up-regulated compared to only 8 genes that were down-regulated. Gene Ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis showed the involvement of 1233 gene ontology (GO) terms and 35 KEGG metabolic pathways. Among these, important defense pathways were induced and antibiotic biosynthesis was the most enriched one. These findings may explain the underlying preventive and curative activity of PGE against plant diseases.

scholarly journals The Bacteriophage Pf-10—A Component of the Biopesticide “Multiphage” Used to Control Agricultural Crop Diseases Caused by Pseudomonas syringae

Viruses ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 42
Author(s):  
Olesya A. Kazantseva ◽  
Rustam M. Buzikov ◽  
Tatsiana A. Pilipchuk ◽  
Leonid N. Valentovich ◽  
Andrey N. Kazantsev ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Gc Content ◽  
Microscopic Analysis ◽  
Plant Diseases ◽  
Host Cells ◽  
Agricultural Crop ◽  
Electron Microscopic ◽  
Bacterial Diseases ◽  
Dsdna Molecule ◽  
Wide Range

Phytopathogenic pseudomonads are widespread in the world and cause a wide range of plant diseases. In this work, we describe the Pseudomonas phage Pf-10, which is a part of the biopesticide “Multiphage” used for bacterial diseases of agricultural crops caused by Pseudomonas syringae. The Pf-10 chromosome is a dsDNA molecule with two direct terminal repeats (DTRs). The phage genomic DNA is 39,424 bp long with a GC-content of 56.5%. The Pf-10 phage uses a packaging mechanism based on T7-like short DTRs, and the length of each terminal repeat is 257 bp. Electron microscopic analysis has shown that phage Pf-10 has the podovirus morphotype. Phage Pf-10 is highly stable at pH values from 5 to 10 and temperatures from 4 to 60 °C and has a lytic activity against Pseudomonas strains. Phage Pf-10 is characterized by fast adsorption rate (80% of virions attach to the host cells in 10 min), but has a relatively small number of progeny (37 ± 8.5 phage particles per infected cell). According to the phylogenetic analysis, phage Pf-10 can be classified as a new phage species belonging to the genus Pifdecavirus, subfamily Studiervirinae, family Autographiviridae, order Caudovirales.

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