Effect of Aging on Free Amino Acids and Electrolytes in Leg Skeletal Muscle

1979 ◽  
Vol 56 (5) ◽  
pp. 427-432 ◽  
Author(s):  
P. Möller ◽  
J. Bergström ◽  
S. Eriksson ◽  
P. Fürst ◽  
K. Hellström
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Statistical Significance ◽  
Plasma Concentrations ◽  
The Elderly ◽  
Essential Amino Acids ◽  
Elderly Men ◽  
Healthy Elderly ◽  
The Difference

1. The concentrations of electrolytes and free amino acids in plasma and the quadriceps femoris muscle were studied in ten apparently healthy elderly men, 52–77 years of age. The results were compared with those previously recorded for men 20–36 years old. 2. The two groups of subjects did not differ with regard to serum electrolytes and intracellular water content but the extracellular water in the older subjects exceeded that of the younger group by about 50%. The muscle specimens of the elderly men were also characterized by a 40% elevation of their total contents of Na+ and Cl−, whereas the content of K+ and Mg2+ was almost identical in both groups. 3. The means recorded for the plasma concentrations of most amino acids tended to be higher in the elderly men. The differences reached statistical significance for tyrosine, histidine, valine, lysine and total essential amino acids. In keeping with the findings in plasma, the amino acid concentrations in the muscle of the older group tended to exceed those of the younger ones. The difference reached statistical significance with regard to total amino acids, essential and non-essential amino acids, aspartate, alanine, citrulline, histidine, arginine, leucine and lysine. The various mechanisms that may contribute to these findings are discussed.

scholarly journals Changes in the plasma concentrations of free amino acids in relation to egg formation in the hen

1970 ◽  
Vol 24 (4) ◽  
pp. 1071-1081 ◽  
Author(s):  
T. G. Taylor ◽  
J. J. Waring ◽  
R. K. Scougall
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Egg Production ◽  
Nutritive Value ◽  
Plasma Concentrations ◽  
Essential Amino Acids ◽  
Egg Formation ◽  
Amino Acid Requirements

1. The changes occurring in the free amino acids of the plasma of laying hens in relation to egg formation have been investigated in fed and starved hens in three experiments, each with eight birds.2. The mean concentrations of most amino acids and of the totals were higher at night than in the morning.3. In general, egg formation was associated with increases in the concentrations of non- essential and decreases in the concentrations of essential amino acids. Cystine and glutamic acid tended to behave like the essential amino acids.4. After 40 h starvation seven amino acids, particularly serine, histidine and lysine, in- creased in concentration and only three, proline, ornithine and arginine, decreased significantly.5. When eight cocks were injected with oestrogen most of the amino acids increased in con-centration. The essential amino acids (with the exception of phenylalanine), serine, proline, cystine and ornithine showed the greatest increases.6. The results are discussed in relation to the possibility that the voluntary food intake of hens may be influenced by changes in the plasma levels of one or more essential amino acids associated with the synthesis of egg albumen. Arginine appeared to be the only amino acid that might possibly fulfil this role.7. It was concluded that investigations of changes in the free amino acids of hen plasma are unlikely to provide a useful approach to a study of the amino acid requirements or the nutritive value of particular proteins for egg production.

Free Amino Acids in Citrullus vulgaris (Watermelon)

PEDIATRICS ◽  
1984 ◽  
Vol 73 (6) ◽  
pp. 879-879
Author(s):  
T. A. TEDESCO ◽  
S. A. BENFORD ◽  
R. C. FOSTER ◽  
L. A. BARNESS
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Neonatal Period ◽  
Essential Amino Acids ◽  
Solid Foods ◽  
Citrullus Vulgaris ◽  
Keto Acids ◽  
Wet Weight ◽  
Cycle Disorders

To the Editor.— Currently accepted dietary management of citrullinemia and other urea cycle disorders includes protein restriction, sodium benzoate, and dietary supplements of keto acids or essential amino acids with postblock intermediates such as arginine in citrullinemia and arginino-succinic aciduria. When a child survives the neonatal period on such a regimen and solid foods are introduced into the diet, there is at least one fruit that should be avoided, Citrullus Vulgaris, commonly known as watermelon. Quantitation of free amino acids extracted from 1 g wet weight of watermelon fruit yielded the following (in mmoles per gram wet weight): Phenylalanine, 1.25; histidine, 0.24; tryptophan, 0.35; lysine, 0.82; ornithine, 0.32; arginine, 11.36; aspartic acid, 0.97; threonine, 0.74; serine, 1.05; glutamine, 3.86; glutamic acid, 1.38; citrulline, 23.68; alanine, 1.15; valine, 0.17; isoleucine, 1.24; leucine, 0.24.

Milk Protein Quantity and Quality in Low-Birth-Weight Infants: II. Effects on Selected Aliphatic Amino Acids in Plasma and Urine

PEDIATRICS ◽  
1977 ◽  
Vol 59 (3) ◽  
pp. 407-422 ◽  
Author(s):  
David K. Rassin ◽  
Gerald E. Gaull ◽  
Kirsti Heinonen ◽  
Niels C. R. Räihäa
Keyword(s):  
Amino Acids ◽  
Birth Weight ◽  
Low Birth Weight ◽  
Human Milk ◽  
Free Amino Acids ◽  
Free Amino ◽  
Plasma Concentrations ◽  
Low Birth Weight Infants ◽  
Low Protein ◽  
Protein Diets

The optimal quantity and quality of protein for low-birth-weight infants is undefined. In this study, 106 well, appropriate-for-gestational-age, low-birth-weight infants weighing 2,100 gm or less were divided into three gestational age groups and assigned randomly within each age group to one of five feeding regimens: pooled human milk; formula 1 (protein content, 1.5 gm/100 ml, 60 parts bovine whey proteins to 40 parts bovine caseins); formula 2 (3.0 gm/100 ml, 60:40); formula 3 (1.5 gm/100 ml, 18:82); and formula 4 (3.0 gm/100 ml, 18:82). The concentrations of the free amino acids in the plasma and urine of these infants were determined. The plasma concentrations of free amino acids were generally far greater in the infants fed the 3.0-gm/100 ml protein diets than they were in the infants fed pooled human milk. The plasma concentrations of free amino acids of the infants fed the 1.5-gm/100 ml protein diets were intermediate. In general, the concentrations of the free amino acids in the plasma of the infants fed the 3.0-gm/100 ml caseinpredominant formula (F4) were furthest from those fed pooled human milk. Glutamate showed the highest plasma amino acid concentrations in infants fed both the high- and low-protein casein-predominant formulas. This was true despite the fact that the intake of glutamate on the high-protein, whey-predominant formula was twice that on the low-protein, casein-predominant formula. The differences between groups in the essential amino acids in plasma were generally greater than those of the nonessential amino acids. The concentrations of amino acids in the urine tended to parallel those of the plasma.

The effect of starvation and low nitrogen intakes on the concentration of free amino acids in the blood plasma and on the nitrogen metabolism in sheep

1970 ◽  
Vol 21 (5) ◽  
pp. 723 ◽  
Author(s):  
J Leibholz
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Dry Matter ◽  
Control Diet ◽  
Amino Nitrogen ◽  
Essential Amino Acids ◽  
Isoleucine Leucine ◽  
Plasma Urea ◽  
Low Nitrogen

Crossbred wethers were given a control diet (8 g nitrogen, 730 g dry matter daily) or a low nitrogen diet (0.5 g nitrogen, 520 g dry matter daily) or starved, for a 12 or 20 day experimental period. The concentrations of free serine, glutamine, glycine, alanine, histidine, and arginine in the plasma of the starved sheep decreased significantly while the concentrations of lysine, 3-methylhistidine, and isoleucine increased significantly. The ratio of essential to non-essential amino acids increased from 0.35 to 0.56 in the starved sheep. In sheep on the low nitrogen diet, the ratio of essential to non-essential amino acids in the plasma decreased from 0.40 to 0.27, with significant increases in the concentrations of glutanlic acid, glutamine, glycine, isoleucine, leucine, and 3-methylhistidine. Starvation and the low nitrogen diet both resulted in a reduction of the plasma urea concentrations. Starvation and the low nitrogen diet resulted in a 20-50 % reduction in the flow of saliva and a 40-78% increase in the concentration of total nitrogen. This resulted in no significant change in the daily secretion of nitrogen in the saliva. The concentration of urea in the saliva was increased by 3-54%. The concentrations of individual free amino acids in saliva are reported. The nitrogen content of the rumen was reduced, and after 7 days of starvation or on the low nitrogen diet all rumen nitrogen could be attributed to ammonia and free �-amino nitrogen.

Utilization of amino acids by pre-ruminant lambs. III.* The influence of age and the dietary proportions of essential and non-essential amino acids on the levels of urea, ammonia and free amino acids in the blood plasma of milk-fed lambs

1978 ◽  
Vol 29 (1) ◽  
pp. 145 ◽  
Author(s):  
H Dove
Keyword(s):  
Amino Acids ◽  
Plasma Levels ◽  
Free Amino Acids ◽  
Free Amino ◽  
Essential Amino Acids ◽  
High Plasma ◽  
Whole Milk ◽  
Wide Range ◽  
Pattern Characteristic ◽  
Pre Treatment

Jugular blood samples were obtained from 10.5 kg and 28 kg lambs receiving a diet of reconstituted cows' whole milk. The lambs were then given diets in which the proportion of essential amino acids (BAA) in the dietary crude protein was altered over a wide range. A second blood sample was taken after lambs had received such diets for 12 days. Plasma obtained from these samples was analysed for free amino acids, urea and ammonia. The pattern of plasma free amino acids (PFAA) in lambs given reconstituted cows' whole milk is described. In both the pre-treatment and post-treatment samples, the heavier lambs appeared to have lower plasma levels of all EAA, and high plasma levels of glycine, serine, urea and ammonia. In the lighter lambs, there were pronounced responses of PFAA levels to changes in the dietary proportion of EAA. At low proportions, the levels of most EAA in plasma were low. Lysine and phenylalanine were exceptions. In addition, levels of many non-essential amino acids (non-EAA), particularly serine and glycine, were high. At high proportions of EAA, plasma levels of all EAA, especially methionine, rose markedly. Within the non-EAA, serine, proline and glycine were reduced, while taurine and cystathionine increased. In the plasma of the heavier lambs, the response of some amino acids to a given dietary change differed from the response in the lighter lambs. This was especially true of methionine, tyrosine, phenylalanine and arginine. There was also marked between-animal variation in plasma levels. When expressed as molar proportions of total PFAA, results were similar to those of the lighter lambs. There was a pronounced similarity between the response of the PFAA to diets with a low proportion of EAA, and the PFAA pattern characteristic of developing kwashiorkor. __________________ *Part II, Aust. J. Agric. Res., 28, 933 (1977).

scholarly journals Use of plasma-free amino acids as biomarkers for detecting and predicting disease risk

2020 ◽  
Vol 78 (Supplement_3) ◽  
pp. 79-85
Author(s):  
Kenji Nagao ◽  
Takeshi Kimura
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino ◽  
Disease Risk ◽  
The Elderly ◽  
Essential Amino Acids ◽  
Clinical Findings ◽  
Amino Acid Supplementation ◽  
Diabetes Prediction ◽  
Plasma Free Amino Acids

Abstract This paper reviews developments regarding the use of plasma-free amino acid (PFAA) profiles as biomarkers for detecting and predicting disease risk. This work was initiated and first published in 2006 and was subsequently developed by Ajinomoto Co., Inc. After commercialization in 2011, PFAA-based tests were adopted in over 1500 clinics and hospitals in Japan, and numerous clinician-led studies have been performed to validate these tests. Evidence is accumulating that PFAA profiles can be used for diabetes prediction and evaluation of frailty; in particular, decreased plasma essential amino acids could contribute to the pathophysiology of severe frailty. Integration of PFAA evaluation as a biomarker and effective essential amino acid supplementation, which improves physical and mental functions in the elderly, could facilitate the development of precision nutrition, including personalized solutions. This present review provides the background for the technology as well as more recent clinical findings, and offers future possibilities regarding the implementation of precision nutrition.

Effect of diet and infusion of volatile fatty acids into the rumen on the concentration of plasma free amino acids in sheep

1982 ◽  
Vol 48 (3) ◽  
pp. 519-526 ◽  
Author(s):  
J. R. Mercer ◽  
E. L. Miller
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino ◽  
Volatile Fatty Acids ◽  
Plasma Concentrations ◽  
Essential Amino Acids ◽  
Protein Nitrogen ◽  
Post Infusion ◽  
Limiting Amino Acid ◽  
The Mean

1. The effect of supplementing barley diets with urea (U), extracted decorticated groundnut meal (GNM) or Peruvian fish meal (PFM) on plasma free amino acid concentrations in sheep have been examined and the first limiting amino acid has been indicated by measuring the changes in the concentration of the plasma essential amino acids (PEAA) during a rumen infusion of a volatile fatty acid (VFA) mixture.2. Three wethers fitted with rumen and re-entrant duodenal cannulas were given isonitrogenous, isoenergetic diets containing (g/kg dry matter (DM)) U 20, GNM 106 or PFM 78, the crude protein (nitrogen × 6.25) contents being 139, 145 and 148 respectively. The sheep were fed hourly, the mean daily dm intake being 0.634 kg.3. Plasma concentrations of valine, threonine, lysine, isoleucine and leucine were linearly related to their concentrations in duodenal digesta.4. A VFA mixture was infused into the rumen for 6 h to supply (mmol/min) acetate 1.47, propionate 0.22 and n-butyrate 0.27. Blood samples were taken 6 h before, during and 12 h after the end of the infusion.5. The concentration of all PEAA decreased relative to the pre-infusion and post-infusion controls but there were no significant differences between diets.6. The mean decreases in concentration averaged over all three diets showed that the decrease in concentration of methionine (41.5%) was far greater than for any other essential amino acid suggesting that under these conditions methionine was the first limiting amino acid.

Dipeptide utilization by starter streptococci

1977 ◽  
Vol 44 (2) ◽  
pp. 309-317 ◽  
Author(s):  
B. A. Law
Keyword(s):  
Amino Acids ◽  
Transport System ◽  
Free Amino Acids ◽  
Free Amino ◽  
Essential Amino Acids ◽  
Exponential Phase ◽  
Whole Cells ◽  
Streptococcus Cremoris ◽  
Defined Media ◽  
Hydrolysis Of

SummaryOf 8 strains ofStreptococcus cremoristested, 5 grew almost as well in defined media in which various essential amino acids were supplied in dipeptides as they did in media containing the equivalent free amino acids. The remainder grew poorly or not at all in the peptide-containing media. Growth of peptide-utilizing strains was inhibited by also including structurally-related dipeptides in the medium, presumably due to competition for uptake by transport system carriers. Both types of starters produced cell-free dipeptidases recoverable from the medium of exponential phase cultures. Addition of the partly-purified extracellular dipeptidases to dipeptidecontaining test media initiated growth in strains unable to use peptides.Str. lactisgrew in defined peptide media, but the further addition of structurally-related dipeptides did not inhibit growth, either bcause each dipeptide was transported by a specific carrier or because peptides were hydrolysed extracellularly. The presence of cell-bound extracellular dipeptidase was indicated by the hydrolysis of dipeptides with washed whole cells in buffer. This was not observed withStr. cremorisstrains.

Intracellular Free Amino Acids in Muscle Tissue of Patients with Chronic Uraemia: Effect of Peritoneal Dialysis and Infusion of Essential Amino Acids

1978 ◽  
Vol 54 (1) ◽  
pp. 51-60 ◽  
Author(s):  
J. Bergström ◽  
P. Fürst ◽  
L.-O. Norée ◽  
E. Vinnars
Keyword(s):  
Amino Acids ◽  
Peritoneal Dialysis ◽  
Amino Acid ◽  
Muscle Tissue ◽  
Free Amino Acids ◽  
Free Amino ◽  
Essential Amino Acids ◽  
Chronic Uraemia ◽  
Muscle Water ◽  
Intracellular Concentrations

1. Free amino acids were determined in the plasma and in the muscle tissue of 14 patients with chronic uraemia; eight were not on dialysis and six were having regular peritoneal dialysis. The concentration of each amino acid in muscle water was calculated with the chloride method. 2. In both groups of patients there were low intracellular concentrations of threonine, valine, tyrosine and carnosine, and high glycine/valine and phenylalanine/tyrosine ratios. Both groups of patients had increased amounts of 1- and 3-methylhistidine in plasma and in muscle water. 3. The non-dialysed patients had low intracellular concentrations of lysine, and the dialysed patients had high intracellular concentrations of lysine, isoleucine, leucine and of some of the non-essential amino acids. 4. After peritoneal dialysis for 22 h, the plasma concentration of several amino acids decreased but the intracellular concentrations of most amino acids did not change significantly. 5. Intravenous administration of essential amino acids and histidine during the last 4 h of dialysis increased in muscle the total free amino acids, the ratio of essential to non-essential amino acids and the valine and phenylalanine concentrations. 6. The results demonstrated that the plasma and muscle concentrations of several amino acids are grossly abnormal in chronic uraemia. Non-dialysed and dialysed patients exhibit important differences, especially in the intracellular amino acid patterns. Infusion of essential amino acids may result in enhancement of protein synthesis.

scholarly journals Quantification of Phytochemicals from CommercialSpirulinaProducts and Their Antioxidant Activities

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Naif Abdullah Al-Dhabi ◽  
Mariadhas Valan Arasu
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
High Performance ◽  
Antioxidant Activities ◽  
Analytical Techniques ◽  
Essential Amino Acids

The present study aimed to profile the polyunsaturated fatty acids, sugars, free amino acids, and polyphenols in 37 varieties ofSpirulinacommonly available in the market using gas chromatography and high performance liquid chromatography. In addition, the biological potentials of theSpirulinasamples were evaluated by analysing thein vitroantioxidant activities using various analytical techniques. The analyses revealed the presence of 13 polyunsaturated fatty acids, 18 amino acids, 7 sugars, and polyphenols. The polyunsaturated fatty acids contents were varied betweenSpirulinasamples. The total polyunsaturated fatty acids amount was 4.25 mg/100 g, and the average among of sapienic acid detected was 2.25 mg/100 g, which was followed by linoleic acid (16.7%) andγ-linolenic acid (14%). Among the 7 sugars, the hexose levels were the highest (73.85%). The total amino acids contents ranged from 11.49 to 56.14 mg/100 g, and the individual essential amino acids accounted for 17% to 39.18%. The “natural” tablets exhibited the highest polyphenols levels (24 mg/g). All of theSpirulinasamples expressed dose-dependent antioxidant activities. The polyunsaturated fatty acids, sugars, free amino acids, and polyphenols contents varied widely, and the variations in these compounds between theSpirulinasamples were significant.

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