Chitosan-Ag nanoparticle antifungal activity against Fusarium sp., causal agent of wilt disease on chili

Abstract Chitosan is a polysaccharides compound derived from nature and has an amine group and a hydroxyl group that gives it unique physicochemical properties, which are polychationic and chelating agent. This shows that chitosan can be used as a stabilizer and reducer of Ag nanoparticles. Unlike other studies, chitosan that was used in this study was derived from Black Soldier Fly exuviae which has been through demineralization, deproteination, depigmentation, and deacetylation processes. This study aims to obtain an alternative environmentally friendly fungicide with Ag-chitosan nanoparticle formulation which has antifungal activity against Fusarium sp. that causes wilt disease in chili plants. We analysed particle size, functional group by FTIR, and antifungal test against Fusarium sp. on 1000, 750, 500, 250, and 100 ppm of chitosan-Ag nanoparticle. The chitosan-Ag nanoparticles have a particle size of 188.4 nm with a polydispersity index of 0.344. FTIR analysis showed that Ag+ ions are successfully grafted into the chitosan matrix. All the chitosan-Ag nanoparticle concentration tested showed significant results with negative and positive control, except the concentration of 100 ppm which is not significantly differ from negative control. The antifungal test showed a concentration of 750 ppm chitosan-Ag nanoparticles had a growth inhibition of 72.17%.

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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Sintering of Silver Nanoparticles at Room-Temperature for Conductive Ink Applications

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.775.144 ◽

2018 ◽

Vol 775 ◽

pp. 144-148 ◽

Cited By ~ 4

Author(s):

Bethel Faith Y. Rezaga ◽

Mary Donnabelle L. Balela

Keyword(s):

Particle Size ◽

Ag Nanoparticles ◽

Room Temperature ◽

Average Diameter ◽

Aqueous System ◽

Ag Nanoparticle ◽

Nacl Solution ◽

Cellulose Acetate Film ◽

Sintered Ag ◽

Nanoparticle Ink

Silver (Ag) nanoparticles synthesized in an aqueous system was sintered at room temperature using NaCl solution. The Ag nanoparticles have an average diameter of about 24 nm. After dispersing the Ag nanoparticles in 50mM NaCl solution, a significant increase in particle size to about 206 nm was observed. On the other hand, the particle size was also increased to about 175 nm when the Ag nanoparticles were printed and then 50mM NaCl solution was dropped onto the printed Ag nanoparticles. The enlargement of particle size was accompanied by the increase in conductivity of the Ag nanoparticle ink. The resistance was reduced from 57.7 to 6.5 and 6.7 ohms for the as-prepared and sintered Ag nanoparticles using two different treatments, respectively. The sintered Ag nanoparticle ink formulation exhibit high conductivity when drawn on both cellulose acetate film and bond paper even after bending and folding of the substrates.

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Fusing of Silver Nanoparticles at Room Temperature Using Halide Solutions for Conductive Inks

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.833.181 ◽

2020 ◽

Vol 833 ◽

pp. 181-185 ◽

Cited By ~ 1

Author(s):

Bethel Faith Y. Rezaga ◽

Mary Donnabelle L. Balela

Keyword(s):

Particle Size ◽

Ag Nanoparticles ◽

Room Temperature ◽

Average Diameter ◽

Aqueous System ◽

Ag Nanoparticle ◽

Nacl Solution ◽

Halide Solution ◽

Sintered Ag ◽

Nanoparticle Ink

Fusing of silver (Ag) nanoparticles synthesized in an aqueous system was observed at room temperature using halide solutions. The as-synthesized Ag nanoparticles have an average diameter of about 24 nm. After dispersing the Ag nanoparticles in a halide solution, a significant increase in particle size to about 188-197 nm was observed. The enlargement of particle size was accompanied by the increase in conductivity of the Ag nanoparticle ink. The resistance was reduced from 110 kiloohms to 35 and 9.3 ohms for the as-prepared and sintered Ag nanoparticles using NaBr and NaCl solution, respectively.

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Uji Aktivitas Antifungi Ekstrak Rimpang Kencur (Kaempferia galanga L.) Terhadap Saprolegnia sp. Secara In Vitro [Antifungal Activity Test Of Kaempferia galanga L. Extract To Saprolegnia sp. By In Vitro]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v5i1.11420 ◽

2019 ◽

Vol 5 (1) ◽

pp. 23

Author(s):

Rahayu Kusdarwati, Ayu Ratnaningtyas, Dewa Ketut Meles

Keyword(s):

Minimum Inhibitory Concentration ◽

Antifungal Activity ◽

Inhibitory Concentration ◽

Positive Control ◽

Negative Control ◽

Fresh Water Fish ◽

Minimum Fungicidal Concentration ◽

Kaempferia Galanga ◽

Activity Test

Abstract Saprolegnia sp. is a fungus that causes the Saprolegniasis disease can infection eggs and fresh water fish. Treatment Saprolegniasis done using chemical drugs, however the use of drugs is bad for the environment and biota. The purpose of the research was to determined the antifungal activity include a minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) from Kaempferia galanga L. to Saprolegnia sp. by in vitro. This research used 9 different concentrations of Kaempferia galanga L extract were 50%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0, 39%, 0.2%, positive control used H2O2 3% and negative control used DMSO 10%. The results showed that the extract of Kaempferia galanga L had an antifungal activity were inhibits and kill with minimum inhibitory concentration (MIC) was 0.39% equivalen with 3,9 mg/ml and minimu fungicidal concentration (MBC) was 1.56% equivalen with 15,6 mg/ml. The existence of antifungal activity against Saprolegnia sp. by in vitro caused by some active compounds from the extracts of the Kaempferia galanga L. are polyphenolic compounds, flavonoin, saponins and essential oils.

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Coarse, but not finely ground, dietary fibre increases intestinalFirmicutes:Bacteroidetesratio and reduces diarrhoea induced by experimental infection in piglets

British Journal Of Nutrition ◽

10.1017/s0007114511005216 ◽

2011 ◽

Vol 108 (1) ◽

pp. 9-15 ◽

Cited By ~ 31

Author(s):

Francesc Molist ◽

Edgar Garcia Manzanilla ◽

José Francisco Pérez ◽

Charles Martin Nyachoti

Keyword(s):

Particle Size ◽

Wheat Bran ◽

Experimental Infection ◽

Dietary Fibre ◽

Positive Control ◽

Negative Control ◽

Volatile Fatty Acid Concentration ◽

Before And After ◽

Weaning Piglets ◽

Gut Microbial Community

Using dietary fibre to control childhood diarrhoea has rarely been discussed. However, dietary fibre is being proposed to prevent diarrhoea in piglets. The present study aimed to study the effects of introducing fibre in the post-weaning piglet diet and its particle size on the intestinal ecosystem before and after an experimental infection withEscherichia coli. A total of thirty-six post-weaning piglets were assigned to four experimental diets: a negative control (NC) diet, the same diet with 4 % wheat bran coarse (WBc) particle size or finely milled (WBF) and a positive control (PC) diet with an antibiotic. On day 9, animals were challenged withE. coli. Faecal and digesta samples were obtained before and after the experimental infection and changes in the microbial ecosystem were measured. Animals fed the WBc and the PC diets showed a significant reduction in the faecal score compared with the NC diet. The inclusion of WBc in the diet increased total volatile fatty acid concentration, reduced Bacteroidetes in the faeces before and after the experimental infection compared with the NC diet and increased Firmicutes at the end of the experiment. Based on the results, diarrhoea scours and the composition of the pig gut microbial community are modified by the inclusion of a relatively small amount of wheat bran in the diet, being the physical presentation of the fibre a determinant of that difference.

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FRAKSI ETANOL EKSTRAK KULIT DURIAN (Durio zibethinus L.) SEBAGAI ANTIFUNGI TERHADAP Trichophyton mentagrophytes DAN Candida albicans

JFL : Jurnal Farmasi Lampung ◽

10.37090/jfl.v8i1.84 ◽

1970 ◽

pp. 28-38

Author(s):

Yuli Wahyu Tri Mulyani ◽

Subur Widodo ◽

Lisa Selviani

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Trichophyton Mentagrophytes ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Bark Extract ◽

Chromatography Method ◽

Durio Zibethinus ◽

Ethanol Fraction

Durian is a fruit that is in demand by the community, but during durian season environmental problems arise due to waste from skin of durian. The aimed of the study was to prove the antifungal activity of the ethanol fraction of durian skin against Trichophyton mentagrophytes and Candida albicans and to determine the active active compounds inhibiting T. mentagrophytes and C. albicans fungi. Durian skin was extracted by maceration method, then fractionated used ethanol, chloroform, and n-hexane solvents. Ethanol fraction was tested on T. mentagrophytes and C. albicans fungi used the disc method with a concentration of 10%, 20%, 40%, 60%, 80%, 100%, distilled water as a negative control, ketoconazole as a positive control. The best inhibition zone is fungi T. mentagrophytes concentration 100% ( 9.23 mm) negative control (0 mm), positive control (32.96 mm), fungi C. albicans the best inhibition zone is concentration of 100% (8.30 mm), positive control (17.17 mm) negative controls (0 mm).The ethanol fraction was screened by the Thin Layer Chromatography method and positively contained flavonoids (rf 0.7), tannins (rf 0.58), saponins (rf 0.76) and alkaloids (rf 0.64). Minimum Inhibitory Concentrations of T. mentagrophytes were 7% and C. albicans were 8%. The most effective active compound growht of T. mentagrophytes is tannins (rf 0.7) and for C. albicans is flavonoid (rf 0.6). The ethanol fraction of durian bark extract has antifungal activity against T. mentagrophytes and C. albicans. Keywords: Antifungi, Candida albicans, ethanol fraction skin of durian, Trichophytonmentagrophytes

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Effectiveness of Ethanol Extract among Three Ganoderma Species From Lombok in Inhibition Growth of Candida albicans and Cryptoccocus neoformans

Journal of Science and Science Education ◽

10.29303/jossed.v1i1.453 ◽

2020 ◽

Vol 1 (1) ◽

pp. 35

Author(s):

Fitria Rizka Hidayat ◽

Sukiman Sukiman ◽

Ernin Hidayati ◽

Sarkono Sarkono ◽

Bambang Fajar Suryadi ◽

...

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Large Diameter ◽

Ethanol Extract ◽

Pathogenic Fungi ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

High Concentration ◽

Opportunistic Pathogenic

Candida albicans and Cryptoccocus neoformans are opportunistic pathogenic fungi that cause infectious diseases that are the world's biggest health problems. The use of antibiotics is one way to overcome the spread of the infection and cause microbial resistance. Ganoderma is one of the many macrophages found on Lombok's island, and studies of its antifungal activity have not been carried out. The purpose of this study was to determine the antifungal potential and the effect of different concentrations of ethanolic extracts of three Ganoderma species on C. albicans and C. neoformans. Ganoderma samples were obtained from Suranadi Taman Wisata Alam (TWA), Sesaot TWA, Tunak Mountain TWA, Kerandangan TWA, and Pusuk Forest. Ganoderma extraction was carried out by the maceration method using ethanol 95% solvent. The extract concentrations used are 20%, 40%, 60% and 80%. This research was conducted using the wells method with metronidazole as a positive control and 50% DMSO as a negative control. The parameter measured is the large diameter of the inhibition zone formed around the well. The results obtained are the three species of Ganoderma have antifungal activity against test fungi, and different levels of concentration affect inhibition. The amount of the inhibition zone is directly proportional to the high concentration of the extract. All three Ganoderma species are more effective in inhibiting the growth of C. neoformans compared with Candida albicans

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Aktivitas Anti Cendawan Ekstrak Daun Sereh Wangi (Cymbopogon nardus L.) Terhadap Colletotrichum sp Penyebab Penyakit Antraknosa Pada Buah Cabai (Capsicum annum L.) Secara In Vitro Dan In Vivo

Agrologia ◽

10.30598/a.v4i1.220 ◽

2015 ◽

Vol 4 (1) ◽

Author(s):

Mohamad Ana Syabana ◽

Andree Saylendra ◽

Deri Ramdhani

Keyword(s):

Weight Loss ◽

Antifungal Activity ◽

In Vitro Study ◽

Positive Control ◽

Negative Control ◽

Control Treatment ◽

Capsicum Annum ◽

Anti Fungal

The aim of this research was to examine in vitro and in vivo anti fungal activity of citronella leaves extract against Colletotrichum sp caused antrachnose disease on chilli. The in vitro and in vivo research used randomized completely design (RCD) with one factor and five level. The factor was citronella leaves extract and the level were 0,1 % (v/v), 0,2 % (v/v), 0,3 % (v/v), 0,4 % (v/v), 0,5 % (v/v). Negative control treatment was conducted by growing Colletotrichum sp on PDA (in vitro) and chili (in vivo) without citronella leaves extract while positive control was conducted by growing Colletotrichum sp on PDA (in vitro) and chili (in vivo) containing synthetic fungicide. The in vitro study showed that the higher concentration of citronella leaves extract caused higher growth inhibition of Colletotrichum sp. Whereas the in vivo study resulted that higher concentration of citronella leaves extract caused lower incubation period of Colletotrichum sp, intensity of disease and weight loss of chillies. The highest concentration of citronella leaves extract (0,5%) has higher antifungal activity compared to other treatments and negative control while lower than positive control.

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ANTIFUNGAL EXTRACT ACTIVITYOF ISOLATE Nocardia sp. ATS-4.1 AGAINST Candida albicans InaCC-Y116

BERITA BIOLOGI ◽

10.14203/beritabiologi.v19i3a.3868 ◽

2020 ◽

Vol 19 (3A) ◽

Author(s):

Abdullah Abdullah ◽

Rahmawati Rahmawati ◽

Rikhsan Kurniatuhadi

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Fungal Infections ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Mueller Hinton ◽

Nocardia Sp ◽

Mueller Hinton Agar ◽

Good Concentration

Candida albicans is a microorganism that knows as caused of candidiasis. Nocardia is known to have the ability to produce antifungal bioactive compounds to overcome cases of fungal infections. This research aims to determine the presence of antifungal activity and the good concentration from crude extract of Nocardia sp. ATS-4.1 to inhibit C. albicans InaCC-Y116 and. Antifungal activity test of isolate Nocardia sp. ATS-4.1 was performed using a well diffusion method on Mueller Hinton Agar (MHA) media with a concentration of 92%, 94%, 96%, 98%, 100% and nystatin (positive control) 0,0125% and DMSO 10% (negative control) incubated at 37 ᵒC for 24-48 hours. The results showed that the extract of Nocardia sp. ATS-4.1 can inhibit the growth of C. albicans. Nocardia sp. ATS-4.1 isolate extract concentration of 96% with a resistance diameter of 13.63 ± 0.53 mm with a strong category against the growth of C. albicans InaCC-Y116.

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Aktivitas Aktivitas Antijamur Ekstrak Etanol Daun Matoa (Pometia Pinnata J.R. & G.Forst) Terhadap Pertumbuhan Jamur Trichophyton Mentagrophytes

Photon: Jurnal Sain dan Kesehatan ◽

10.37859/jp.v11i2.2705 ◽

2021 ◽

Vol 11 (2) ◽

pp. 137-148

Author(s):

Wahyu Margi Sidoretno ◽

Mirna Gustari

Keyword(s):

Antifungal Activity ◽

Ethanolic Extract ◽

Trichophyton Mentagrophytes ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Laboratory Methods ◽

Experimental Laboratory ◽

Bacteria And Fungi

The matoa plant is widely used to treat infectious diseases caused by bacteria and fungi. The purpose of this study was to determine the antifungal activity of the ethanolic extract of matoa (Pometia pinnata) leaves on the growth of Trichophyton mentagrophytes at various concentrations 10%, 20%, and 30%. Trichophyton mentagrophytes are a type of fungus that belongs to the dermatophyte group that causes dermatophytoses (ringworm). The experimental laboratory methods were used in this study. The antifungal activity was determined by using the agar diffusion method. Ketoconazole was used as the positive control, and DMSO was used as the negative control. This study showed the inhibition zone of the extract with concentrations 10%, 20%, 30% were 23.05 mm, 24.86 mm, 25.05 mm, respectively. Ketoconazole as positive control showed the inhibition zone 28.56 mm. From the results, it can be concluded that the ethanolic extract of the leaves of matoa (Pometia pinnata) has potential antifungal activity against Trichophyton mentagrophytes.

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