Aktivitas Aktivitas Antijamur Ekstrak Etanol Daun Matoa (Pometia Pinnata J.R. & G.Forst) Terhadap Pertumbuhan Jamur Trichophyton Mentagrophytes

2021 ◽  
Vol 11 (2) ◽  
pp. 137-148
Author(s):  
Wahyu Margi Sidoretno ◽  
Mirna Gustari
Keyword(s):  
Antifungal Activity ◽  
Ethanolic Extract ◽  
Trichophyton Mentagrophytes ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Laboratory Methods ◽  
Experimental Laboratory ◽  
Bacteria And Fungi

The matoa plant is widely used to treat infectious diseases caused by bacteria and fungi. The purpose of this study was to determine the antifungal activity of the ethanolic extract of matoa (Pometia pinnata) leaves on the growth of Trichophyton mentagrophytes at various concentrations 10%, 20%, and 30%. Trichophyton mentagrophytes are a type of fungus that belongs to the dermatophyte group that causes dermatophytoses (ringworm). The experimental laboratory methods were used in this study. The antifungal activity was determined by using the agar diffusion method. Ketoconazole was used as the positive control, and DMSO was used as the negative control. This study showed the inhibition zone of the extract with concentrations 10%, 20%, 30% were 23.05 mm, 24.86 mm, 25.05 mm, respectively. Ketoconazole as positive control showed the inhibition zone 28.56 mm. From the results, it can be concluded that the ethanolic extract of the leaves of matoa (Pometia pinnata) has potential antifungal activity against Trichophyton mentagrophytes.  

scholarly journals FRAKSI ETANOL EKSTRAK KULIT DURIAN (Durio zibethinus L.) SEBAGAI ANTIFUNGI TERHADAP Trichophyton mentagrophytes DAN Candida albicans

1970 ◽  
pp. 28-38
Author(s):  
Yuli Wahyu Tri Mulyani ◽  
Subur Widodo ◽  
Lisa Selviani
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Trichophyton Mentagrophytes ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Bark Extract ◽  
Chromatography Method ◽  
Durio Zibethinus ◽  
Ethanol Fraction

Durian is a fruit that is in demand by the community, but during durian season environmental problems arise due to waste from skin of durian. The aimed of the study was to prove the antifungal activity of the ethanol fraction of durian skin against Trichophyton mentagrophytes and Candida albicans and to determine the active active compounds inhibiting T. mentagrophytes and C. albicans fungi. Durian skin was extracted by maceration method, then fractionated used ethanol, chloroform, and n-hexane solvents. Ethanol fraction was tested on T. mentagrophytes and C. albicans fungi used the disc method with a concentration of 10%, 20%, 40%, 60%, 80%, 100%, distilled water as a negative control, ketoconazole as a positive control. The best inhibition zone is fungi T. mentagrophytes concentration 100% ( 9.23 mm) negative control (0 mm), positive control (32.96 mm), fungi C. albicans the best inhibition zone is concentration of 100% (8.30 mm), positive control (17.17 mm) negative controls (0 mm).The ethanol fraction was screened by the Thin Layer Chromatography method and positively contained flavonoids (rf 0.7), tannins (rf 0.58), saponins (rf 0.76) and alkaloids (rf 0.64). Minimum Inhibitory Concentrations of T. mentagrophytes were 7% and C. albicans were 8%. The most effective active compound growht of T. mentagrophytes is tannins (rf 0.7) and for C. albicans is flavonoid (rf 0.6). The ethanol fraction of durian bark extract has antifungal activity against T. mentagrophytes and C. albicans. Keywords: Antifungi, Candida albicans, ethanol fraction skin of durian, Trichophytonmentagrophytes

scholarly journals Pengujian Aktivitas Antibakteri Hidrogel Ekstrak Etanol Daun Tagetes erecta L

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 96
Author(s):  
Hosea Jaya Edy ◽  
Marchaban Marchaban ◽  
Subagus Wahyuono ◽  
Agung Endro Nugroho
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Tagetes Erecta ◽  
Tagetes Erecta L

Kandungan kimia flavonoid dan fenolik dalam ekstrak daun T.erecta diketahui memiliki aktivitas antibakteri yang baik. Pengunaan ekstrak daun T.erecta sebagai zat aktif sediaan hidrogel akan sangat mempermudah pemanfaatan dalam proses terapi oleh masyarakat. Tujuan dalam penelitian ini adalah melihat kemampuan aktivitas antibakteri sediaan hidrogel dengan zat aktif ekstrak daun T.erecta. Dalam penelitian ini digunakan 6 variasi konsentrasi ekstrak yang terkandung pada sediaan hidrogel yaitu 1.5; 2.0; 2.5; 3.0; 3.5; dan 4.0 %. Pengujian aktivitas antibakteri menggunakan metode difusi sumuran terhadap bakteri Staphylococcus aureus dan Escherichia coli. Aktivitas antibakteri terbesar ditunjukkan oleh sediaan hidrogel dengan konsentrasi ekstrak daun T.erecta 2.5%. Diameter zona hambat yang terbentuk terhadap pertumbuhan E.coli adalah 15.33 mm sedangkan diameter zona hambat terhadap S.aureus adalah 17.87 mm. Untuk kontrol positif digunakan bioplacenton® dan untuk kontrol negatif digunakan basis hidrogel tanpa kandungan ekstrak T.erectaFlavonoid and phenolic on ethanolic extract of T.erecta L has been antibacterial activity. The aim of this research was to determinate of antibacterial from hydrogel with ethanolic extract of T.erecta L. This research was begun making hydrogels with six variations of ethanolic extract of T.erecta L were 1.5; ; 2.0; 2.5; 3.0; 3.5; and 4.0 %.  Antibacterial activity used hole diffusion method against Staphylococcus aureus and Escherichia coli. Hydrogel containing 2.5 % ethanolic extract of T.erecta L was shown the highest antibacterial activity. The diameters of the inhibition zone against E.coli was 15.33 mm and against S.aureus was 17.87 mm. Positive control used bioplacenton® and negative control was hydrogel without ethanolic extract of T.erecta content

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes

EKOLOGIA ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 31-39
Author(s):  
Oom Komala ◽  
. Ismanto ◽  
Muhammad Alan Maulana
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Dilution Method ◽  
Natural Treatment ◽  
Amomum Compactum

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from  ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used   solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD)  using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were  analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC  was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.

scholarly journals Antiinflammatory Activity of Pagoda Flower (Clerodendrum Paniculatum L.) Ethanol Extract Using Paw Edema Method

2019 ◽  
Vol 7 (6) ◽  
pp. 43-45
Author(s):  
Ihsanul Hafiz ◽  
Mandike Ginting
Keyword(s):  
Antiinflammatory Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Paw Edema ◽  
Laboratory Design ◽  
White Rats ◽  
Experimental Laboratory ◽  
The Difference

Objectives: The purpose of this study was to determine antiinflammatory activity of pagoda flower (Clerodendrum paniculatum L.) ethanolic extract Design: This study uses an experimental laboratory design. This research uses paw edema method by inducing carrageenin in the legs of male white rats as an induction of inflammation. Interventions: The sample used was pagoda flower ethanol extract in various dosages of 25, 50 and 100 mg / kg. As a comparison, acetosal dose 33 mg / kg was used. Na CMC suspension was used as a negative control. Main outcome measure: The results in this study are the difference in the volume of edema volume from rat feet per unit time. The measurement of the rat's leg volume was measured at 30, 60, 120, 180, 240 and 300 minutes. Conclusion: Pagoda flower ethanol extract does not have good anti-inflammatory activity. there were no significant differences between groups except at dose 100 and positive control at minute 300 of negative control.    

scholarly journals The Potential of Mango (Mangifera infica L.) Peel of Apple Varieties By Infusion And Maceration In Inhibiting Pseudomonas aeruginosa And Propionibacterium acnes

2021 ◽  
Vol 4 (1) ◽  
pp. 1-6
Author(s):  
Vifin Putri Rahmawati ◽  
Chylen Setiyo Rini
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mangifera Indica ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Chemical Components ◽  
Extract Concentration ◽  
Potential Test ◽  
Antibacterial Potential

Plants have many chemical components. The use of natural ingredients as an alternative treatment in dealing with diseases, especially acne. One of them is mango (Mangifera indica L.) varieties of apples obtained at the Larangan Main Market in Sidoarjo. This study aims to determine the potential of infusion and maceration of mango skin varieties in inhibiting Pseudomonas aeruginosa and Propionibactrium acne at various concentrations. This antibacterial potential test was carried out using the diffusion method of the wells. The antibacterial potential is characterized by the formation of a clear zone around the well called the inhibition zone. This study uses 10 concentrations namely 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% and Clindamycin as positive control and aquades as negative control. Based on the results of the Two Way ANOVA test data obtained were not normally distributed, therefore a comparison test was performed using the Kruskal-Wallis test with a sign value (α <0.05). This showed that there were significant differences in the use of various concentrations. The maceration extract concentration of 100% is the best concentration to form a zone of inhibition against P. aeruginosa  of 17.9 mm and P. acne bacteria of 13.2 mm. The results of the infusion extract concentration did not form inhibitory zones in both of P. aeruginosa and P. acnes.

scholarly journals The potency of ethanolic extract from corn silk as natural antibiotics for acne-related bacteria: A preliminary study

2022 ◽  
Vol 21 (1) ◽  
pp. 84-89
Author(s):  
Fitri A Nurani ◽  
Nadia RS Rejeki ◽  
Tanti Setyoputri ◽  
Putri K Wardani ◽  
Fatkhan B Ridwan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Acne Vulgaris ◽  
Medical Science ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Traditional Medicines ◽  
Corn Silk

Introduction: Corn silk or known as herb name stigma maydis is an important medicinal botanical in many traditional medicines worldwide, including jamu, an Indonesia traditional medicine. The exploration of corn silk to treat acne vulgaris is still lacking, therefore the current research was conducted to analyze the activity of ethanolic extract from corn silk (EECS) against 3 acne-related bacteria, Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus. Materials and Methods: The antibacterial activity of EECS at concentration range of 10 to 100% v/v was evaluated using the disk diffusion method. As comparation, distilled water was used as a solvent control, while 1% clindamycin was used as a positive control. Results: Shinoda’s test showed that flavonoid was detected in the EECS. The higher concentration of EECS exhibited higher diameter of inhibition zone indicating higher antibacterial activity on P. acnes, while the antibacterial activity of S. epidermidis was not increased at similar concentrations of EECS. The antibacterial activity of EECS against S. aureus decreased at the higher EECS concentration (>70%). Conclusion: Taken together, EECS is a potential as a bioactive source to inhibit the growth of acne-related bacteria P. acne, S. epidermidis and S. aureus. Further investigation is needed to explore the corn silk or stigma maydis as a medicinal botanical in jamu targeted to treat acne vulgaris. Bangladesh Journal of Medical Science Vol. 21(1) 2022 Page : 84-89

scholarly journals UJI EFEKTIFITAS DAUN PANDAN WANGI (PANDANUS AMARYLLIFOIUM ROXB) SEBAGAI ANTIJAMUR TERHADAP PITYSPORUM OVALE

2021 ◽  
Vol 3 (2) ◽  
pp. 81-85
Author(s):  
Saadah Siregar ◽  
Riana Topia
Keyword(s):  
Fungal Growth ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Oil Glands ◽  
Pityrosporum Ovale ◽  
Agar Media ◽  
Metabolite Content ◽  
Pandanus Amaryllifolius

The Pandan Wangi leaf plant is a plant that belongs to the Pandanaceae family. The metabolite content of fragrant pandan leaves (Pandanus amaryllifolius  Roxb.) Alkaloids, flavonoids, saponins, tannins are known to have an antifungal effect. Pityrosporum ovale is a yeast or single-celled fungus that belongs to the genus Malassezia sp, and belongs to the Cryptococcaceae family. Pityrosporum ovale causes superficial dermatomycosis which affects the stratum corneum in the epidermis layer. This fungus is a normal flora on the scalp, but in conditions of hair with excess oil glands, this fungus can thrive. The purpose of this study was to determine the inhibition zone of fragrant pandanus leaves (Pandanus amaryllifolius Roxb.) Concentrations of 10%, 20%, 30%, and 40% of Pytirosporum ovale on Potato Dextrose Agar media by diffusion method using disc paper. The diffusion method was used to measure the diameter of the zone of inhibition against fungal growth. The positive control used was ketoconazole 2% and negative control DMSO 1%. The results of the study using the diameter of the inhibition zone at a concentration of 10% were 7.86 mm, the inhibition zone at a concentration of 20% was 8.53 mm, the inhibition zone at a concentration of 30% was 8.76 mm, and the inhibition zone at a concentration of 40% was 9, 43 mm.

scholarly journals Evaluation of Anti Microbial and Anti Fungal Activity of Acalyphaindica L., Leaf Extract

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Sudhakar Chekuri ◽  
Arun Jyoti B ◽  
Saraswathi JSompaga ◽  
Shivaprasadi Panjala ◽  
Roja Ran Anupalli
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Fungal Activity ◽  
Bacteria And Fungi ◽  
Anti Fungal ◽  
Acalypha Indica

Anti-microbial and anti-fungal activity of different solvent extracts of Acalypha indica (Euphorbeace family) was tested against bacterial pathogens (Pseudomonasaeruginosa, E.Coli, KlebsiellaPneumonia and Staphylococcus aureus) and fungal strains (Candida albicans, Aspergillus niger, Candida tropicalis andCandida kefyr) using the Agar Well diffusion method . It was observed that all the extracts showed positive activity) against bacteria and fungi. Ethanolic extract of Acalypha indica showed more potency against Staphylococcus aureus with an inhibition zone of12.46 (mm) and Methanolic extract exhibited higher activity against E.coli with an inhibition zone of11.26 (mm). Ethanolic extract of Acalypha indica showed prominent antifungal activity against candida albicans with an inhibition diameter of 12.53 (mm) and Aspergillus niger with a diameter of 9.21 (mm) when compared to other solvent extracts. Erythromycin and Ketoconazole were used as positive standards for antimicrobial and anti fungal experiments. In the present study, Ethanol extract showed a varying degree of inhibition to the growth of tested organisms compared to Methanol, Acetone and Chloroform against Bacteria and Fungi. The results confirmed the presence of antibacterial and antifungal compounds in shade dried extracts of Acalypha indica against human pathogenic organisms.

scholarly journals Uji Aktivitas Antibakteri Dari Air Perasan Bawang Putih Terhadap Bakteri Salmonella Typhi

2020 ◽  
Vol 6 (1) ◽  
pp. 9-12
Author(s):  
Olivia Yolanda Pasaribu ◽  
Ade Pryta Simaremare ◽  
Joseph Partogi Sibarani
Keyword(s):  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Laboratory Research ◽  
Diffusion Method ◽  
Minimum Concentration ◽  
Experimental Laboratory ◽  
Negative Controls

Background : Garlic is a plant which is used to treat various types of diseases, especially the ones caused by bacterial infection. Allicin in garlic is one of the active compounds which has an antibacterial effect. Some studies found  that garlic has an inhibitory effect on Salmonella typhi. Objective : This study aimed to determine the antibacterial effect of garlic juice against Salmonella typhi. Methods : This study was  an experimental-laboratory research using disc diffusion method on MHA media. This study used five concentrations, namely 12.5%, 25%, 50%, 75%, and 100%. Amoxicillin and aquadest were used as positive and negative controls. The inhibition zone was determined by observing the clear zone around the disc then the results of the study were compared with positive control with CLSI 2017 guidance table where Salmonella typhi was susceptible when the amoxicillin inhibitory diameter zone was ≥ 17 mm, intermediate was 14-16 mm, and resistant was ≤ 13 mm. Results : The results showed that garlic juice has an antibacterial effect on various consentration. The minimum concentration of garlic juice to inhibit the growth of Salmonella typhi is on 50% with 19.25 mm diameters. Conclusion : There was an antibacterial effect of garlic juice on Salmonella typhi.

scholarly journals 25. The effect of Fingerroot Rhizome (Boesenbergia pandurata) Extract on the Growth of Staphylococcus aureus in Vitro

2019 ◽  
Vol 13 (2) ◽  
Author(s):  
Silvia P.N Keliat ◽  
Darniati Darniati ◽  
Abdul Harris ◽  
Erina Erina ◽  
Rinidar Rinidar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Final Conclusion ◽  
Distilled Water

This study aimed to determine the inhibitory effect and the concentration of fingerroot extract to inhibit Staphylococcus aureus growth. The data was analyzed descriptively. Ampicillin was used as positive control, distilled water was used as negative control, and the treatments were given fingerroot extract with a concentration of 5%, 15%, 25%, 35% and 45%. This study was conducted with three replications. The parameter measured was the diameter of inhibition zone formed by diffusion method. The average diameter of inhibition zone of the fingerroot extract were 15% : 10.3 mm; 25% : 13,6mm; 35% : 18,7mm; 45% ± 21,1mm, and at a concentration of 5% the inhibition zone is not formed. The final conclusion is that the fingerroot extract can inhibit the growth of Staphylococcus aureus. The higher concentration of fingerroot extract, the more extensive inhibition zone formed.

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