scholarly journals Bioactive Compounds and Antioxidant Activity of Cauliflower According to Heat Treatment Method (P06-074-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Eun-Sun Hwang
Keyword(s):  
Heat Treatment ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Science Research ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Cooking Methods ◽  
Cruciferous Vegetables ◽  
Total Polyphenol ◽  
Health Promoting

Abstract Objectives Cauliflower is one of the cruciferous vegetables and contains various physiologically active substances such as glucosinolates, polyphenols, and flavonoids. Unlike ordinary vegetables, cruciferous vegetables are often consumed by cooking through heat treatment. The purpose of this study was to investigate the effects of different heating procedures, in particular steaming and boiling, on glucosinolate, total polyphenol, and total flavonoid concentrations in cauliflower. In addition, antioxidant activity was compared between fresh uncooked, steamed, and boiled cauliflower, which are the main methods of preparing cauliflower before consumption. Methods Glucosinolates in uncooked, steamed, and boiled cauliflower were subjected to high-pressure liquid chromatography. Total phenolic and total flavonoid content and antioxidant activities in cauliflower extracted in both water and 80% ethanol were determined. Results Eight glucosinolate peaks were detected in cauliflower representing glucoiberin, progoitrin, glucoraphanin, sinigrin, gluconapin, glucoiberverin, glucobrassicin, and gluconasturtiin. Boiling cauliflower significantly decreased the glucosinolate, total polyphenol, and total flavonoid concentrations compared to those of uncooked or steamed cauliflower. The results clearly indicated that health-promoting compounds in cauliflower are significantly affected by different cooking methods, showing that uncooked > steamed > boiled. The amounts of total polyphenols and total flavonoids contained within uncooked cauliflower extracted by 80% ethanol were higher than those in the steamed and boiled extracts. The highest antioxidant activity was observed in uncooked cauliflower extracted in 80% ethanol compared to that of water extracts at the same concentration. Steamed and boiled cauliflower extracts showed lower antioxidant activity. Conclusions Based on these results, fresh uncooked cauliflower is optimal in terms of the content of health-promoting compounds and antioxidant activity. It may be desirable to use steaming rather than boiling to minimize the loss of glucosinolates when storing, pretreating, processing, and cooking cruciferous vegetables. Funding Sources This research was supported by the Basic Science Research Program through the National Research Foundation of Korea.

scholarly journals Antioxidant Potential and Cytotoxic Effect of Isoflavones Extract from Thai Fermented Soybean (Thua-Nao)

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7432
Author(s):  
Kanokwan Kulprachakarn ◽  
Supakit Chaipoot ◽  
Rewat Phongphisutthinant ◽  
Narisara Paradee ◽  
Adchara Prommaban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Cell Growth ◽  
Cancer Cell ◽  
Cytotoxic Effect ◽  
Antioxidant Activities ◽  
Bacterial Species ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Cancer Cell Growth

Thua-nao, or Thai fermented soybeans, is a traditional Lanna fermented food in Northern Thailand. It is produced by using a specific bacterial species called Bacillus subtilis var. Thua-nao. We investigated the antioxidant activity and cytotoxic effect of isoflavones from Thua-nao. The phenolic compound contents and total flavonoid contents were determined by spectrophotometry. The antioxidant activity was examined using the ABTS, FRAP, and DPPH assays. The isoflavone contents and phenolic compositions were examined by the high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) techniques. The ability of isoflavones to inhibit human cancer cell growth was assessed by the MTT assay. The total phenolic content, total flavonoid content, and antioxidant activities of the isoflavones were 49.00 ± 0.51 mg GAE/g of dry extract (DE), 10.76 ± 0.82 mg QE/g of DE, 61.03 ± 0.97 µmol Trolox/g of DE, 66.54 ± 3.97 µM FeSO4/g of DE, and 22.47 ± 1.92% of DPPH inhibition, respectively. Additionally, the isoflavone extracts from Thua-nao had high isoflavone contents and polyphenolic compound compositions, especially daidzein and genistein. The isoflavone demonstrated a weak inhibition of MCF-7 and HEK293 cancer cell growth. It has a high antioxidant component, which is beneficial and can be developed for new therapeutic uses. However, further studies on the benefits of Thua-nao should be performed for realizing better and more effective uses soon.

scholarly journals Antioxidant properties of Philippine Bignay (Antidesma bunius (Linn.) Spreng cv. ‘Common’) flesh and seeds as affected by fruit maturity and heat treatment

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 1980-1987
Author(s):  
K.A.T. Castillo-Israel ◽  
K.J.D. Sartagoda ◽  
M.C.R. Illano ◽  
L.E.L. Flandez ◽  
M.C.M. Compendio ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Antioxidant Content ◽  
Common Variety ◽  
Ripe Stage

The effects of maturity (green-unripe, red-ripe, and purple-fully ripe) and heat treatment (blanching at 90±5°C for 2 mins and steaming at 105±5°C for 5 mins) on the antioxidant content and activity of Philippine bignay (Antidesma bunius (Linn.) Spreng cv. ‘common’) flesh and seeds were investigated. The total phenolic content (TPC), total flavonoid content (TFC) and total anthocyanin content (TAC) were determined using the Folin-Ciocalteau assay, aluminum chloride assay, and pH differential technique, respectively, and the antioxidant activity was determined using DPPH, ABTS, and FRAP assays. Results showed that the antioxidant content and activity of bignay increased with maturity. Fully ripe flesh and seeds were found to have the highest antioxidant content and activity regardless of the heat treatment applied. The TPC (8.06±0.15 mg gallic acid equivalents (GAE)/g), TFC (8.10±0.06 mg quercetin equivalents (QE)/g), TAC (4.07±0.03 mg cyanidin-3-glucoside equivalents (CGE)/L extract), and antioxidant activity (ABTS 10.37±0.04 , DPPH 9.60±0.10, FRAP 24.37±0.53 Trolox equivalents (TE)/g) of fully ripe seeds were superior when blanched. On the other hand, the TPC (40.73±0.04 mg GAE/g), TFC (31.54±0.15 mg QE/g), TAC (20.93±0.12 mg CGE/L extract), of fully ripe flesh were superior when not heat-treated while its antioxidant activity (ABTS 34.19±0.19, DPPH 14.01±0.02, FRAP 87.78±1.22 (TE)/g) was highest when blanched but did not vary significantly compared to unheated samples. This study suggested that bignay ‘common’ variety was a good source of antioxidants particularly the flesh at fully ripe stage. Blanching had shown to enhance its antioxidant activities.

scholarly journals Capsaicinoids, Polyphenols and Antioxidant Activities of Capsicum annuum: Comparative Study of the Effect of Ripening Stage and Cooking Methods

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 364 ◽  
Author(s):  
Mansor Hamed ◽  
Diganta Kalita ◽  
Michael E. Bartolo ◽  
Sastry S. Jayanty
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Capsicum Annuum ◽  
Antioxidant Activities ◽  
Dry Weight ◽  
Mature Stage ◽  
Total Phenolic ◽  
Cooking Methods ◽  
Wide Range ◽  
Green Stage

Peppers (Capsicum annuum L.) are an important crop usually consumed as food or spices. Peppers contain a wide range of phytochemicals, such as capsaicinoids, phenolics, ascorbic acid, and carotenoids. Capsaicinoids impart the characteristic pungent taste. The study analyzed capsaicinoids and other bioactive compounds in different pepper cultivars at both the mature green and red stages. The effect of roasting on their nutritional content was also investigated. In the cultivars tested, the levels of capsaicin ranged from 0 to 3636 µg/g in the mature green stage and from 0 to 4820 µg/g in the red/yellow stage. The concentration of dihydrocapsaicin ranged from 0 to 2148 µg/g in the mature green stage and from 0 to 2162 µg/g in the red/yellow stage. The levels of capsaicinoid compounds in mature green and red /yellow stages were either reduced or increased after roasting depending on the cultivar. The ranges of total phenolic and total flavonoids compounds were 2096 to 7689, and 204 to 962 µg/g, respectively, in the green and red/yellow mature stage pods. Ascorbic acid levels in the peppers ranged from 223 to 1025 mg/ 100 g Dry Weight (DW). Both raw and roasted peppers possessed strong antioxidant activity as determined by 2,2-diphenyl-1-picrylhydrazyl) reagent (DPPH, 61–87%) and 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS, 73–159 µg/g) assays. Ascorbic acid and antioxidant activity decreased after roasting in the mature green and red stages, whereas total phenolics and flavonoids increased except in the mature green stage of Sweet Delilah and yellow stage of Canrio.

scholarly journals Correlation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white

2021 ◽  
Vol 4 (4) ◽  
pp. 301-310
Author(s):  
Irma Antasionasti ◽  
Olvie Syenni Datu ◽  
Utami Sasmita Lestari ◽  
Surya Sumantri Abdullah ◽  
Imam Jayanto
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Egg White ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Stable Free Radical ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Myristica Fragrans

The nutmeg (Myristica fragrans) flesh extract has a strong antioxidant activity. Therefore, M. fragrans flesh can be developed for functional drinks which are sources rich in antioxidants good for the prevention and treatment of diseases such as cancer and cardiovascular diseases. However, the tannins' content can cause a bitter and sour taste. Therefore, the tannins content should be reduced by the addition of egg white. The purpose of this study is to find out the comparison of antioxidant activity between a combination of M. fragrans flesh extract with various concentrations of egg white and to correlate its antioxidant activities with tannin, total flavonoid, and total phenolic contents. The antioxidant activities were conducted on M. fragrans flesh extract by using DPPH and ABTS radicals. Tannin, total flavonoid, and total phenolic contents from M. fragrans flesh extract were also tested. The M. fragrans flesh extracts without addition egg white have a strong antioxidant in scavenging the stable free radical ABTS (89.980±0.480 µg/mL) and intermediate antioxidant in scavenging the stable free radical DPPH (105.669±0.102 µg/mL). It is followed accordingly by tannin, total flavonoid, and total phenolic contents, namely 14.034±0.100 %w/w TAE, 26.929±0.129 %w/w QE, and 53.164±0.129 %w/w GAE, respectively. Correlation of tannin, total flavonoid, and total phenolic contents, which inhibited DPPH and ABTS radicals had R2 values of about 89.23-97.63%. It showed that antioxidant activity is strongly influenced by the tannin, total flavonoid, and total phenolic contents. Therefore, precipitation from the tannin-protein bond caused antioxidant activities were decreased.

scholarly journals Distribution of β-Glucan, Phenolic Acids, and Proteins as Functional Phytonutrients of Hull-Less Barley Grain

Foods ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 680 ◽  
Author(s):  
Gordana Šimić ◽  
Daniela Horvat ◽  
Alojzije Lalić ◽  
Daliborka Koceva Komlenić ◽  
Ivan Abičić ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Protein Content ◽  
Phenolic Acids ◽  
High Performance ◽  
Antioxidant Activities ◽  
Barley Grain ◽  
Total Phenolic ◽  
Health Promoting

Two hull-less barley varieties were roller-milled, and breaks (B) and reduction flours (C), shorts, and bran were collected. Shorts, which mainly originate from endosperm cells with a smaller amount of the outer layers, had the largest yield (48.87–51.54%). Ash (0.82–3.10%) and protein (9.95–14.8%) increased from flours toward shorts and bran, while starch decreased (82.31–48.69%). In contrast to clear distribution differences in protein content (bran > shorts > C > B), albumins/globulins content was lowest in bran (0.78–0.90 g/100 gdw), and their distribution between fractions was uneven and genotype dependent. Distribution of hordeins (6.69–10.49 g/100 gdw) was more distinct and generally decreased in order from bran > B > shorts > C. The proportion of nutritionally poor C-hordeins in total hordeins varied from 28.33% to 30.24%, without significant differences between fractions. The β-glucan content varied from 0.80% to 7.49% with decreasing content in the order bran, shorts > C > B. Shorts and bran could be classified as moderate and high β-glucan flour (5.70–7.22%). The total phenolic and antioxidant activities ranged from 0.91 to 2.21 mg GAE/gdw and 28.81–72.06%, respectively. Ferulic and sinapic acids determined by high-performance liquid chromatography (HPLC) were major contributors to the antioxidant activity (45.16–1026.91 ug/gdw and 18.93–206.52 ug/gdw), respectively. The yield and high content of phytonutrients make hull-less barley shorts suitable for the production of health-promoting food and food supplements.

scholarly journals Antibacterial and antioxidant activities of ethanol extract of Artocarpus lacucha Buch-Ham. leaves against Propionibacterium acne

2021 ◽  
Vol 332 ◽  
pp. 08007
Author(s):  
Panal Sitorus ◽  
Mahatir Muhammad ◽  
Henni Cintya ◽  
and Denni Satria
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
The Body ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Degenerative Diseases ◽  
Inhibitor Concentration ◽  
Propionibacterium Acne

Antioxidants can neutralize free radicals that can affect biological systems in the body that can cause degenerative diseases. Antibacterial active molecules have been identified from plants, and these chemicals are being utilized to develop new and promising treatments. The Mobe plant (Artocarpus lacucha Buch-Ham.) is a species of Artocarpus member of the Moraceae family. Includes a high proportion of phenolic compounds (flavonoids and phenolic acid). This research obtained to know about the biological activity of Artocarpus lacucha Buch-Ham and their total flavonoid and total phenolic content. The extract was prepared using the soxhletation technique using ethanol as the solvent. Antibacterial activity was determined using minimum inhibitor concentration. Propionibacterium acne can be inhibited at concentrations ranging from 300 ppm to 25 ppm. The DPPH technique was used to assess antioxidant activity. The samples’ total flavonoid and phenolic content were measured colorimetrically. The DPPH test revealed an antioxidant activity of 23.33 g/mL, which was used to compute the IC50. The extract’s total phenolic (117.44 mg GAE/g) and flavonoid (3.54 mg QE/g) content were determined to be high.

scholarly journals Anti-inflammatory and Antioxidant Activities of the Extracts from Leaves and Stems of Polygonum odoratum Lour

2019 ◽  
Vol 18 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Nittaya Chansiw ◽  
Kamonnaree Chotinantakul ◽  
Somdet Srichairatanakool
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Stem Extract ◽  
Anti Inflammatory

Background: Polygonum odoratum is an indigenous vegetable that has been used as a favoring agent and also used as a Thai traditional medicine to treat flatulence. </P><P> Objective: To analyze active ingredients, total phenolic and total flavonoid contents, antiinflammatory and antioxidant activities from leaf and stem extracts of P. odoratum. </P><P> Methods: Leaves and stems were dried and extracted by using methanol, dichloromethane and water for obtaining Methanolic Leaf Extract (MLE), Methanolic Stem Extract (MTE), Dichloromethane Leaf Extract (DLE), Dichloromethane Stem Extract (DTE), Water Leaf Extract (WLE) and Water Stem Extract (WTE). The extracts were quantified for total phenolic and total flavonoid contents by spectrophotometry and active compounds were analyzed by using GC-MS. Antioxidant activity was determined by ABTS and DPPH radicals scavenging assays. Anti-inflammatory activity was tested by the inhibition of nitric oxide production in RAW 264.7 macrophage cells induced by lipopolysaccharide. </P><P> Results: The DLE exhibited the most potent anti-inflammatory effect by inhibiting nitric oxide production in a concentration-dependent manner (IC50 = 53.75&#177;0.72 &#181;g/mL). MLE exhibited strong antioxidant activity and contained the highest concentration of phenolic compounds (52.59&#177;0.58 mg gallic acid equivalent/g extract) and flavonoid (19.97+0.11 mg quercetin equivalent/g extract). E-15-Heptadecenal and 3, 7, 11, 15-tetramethyl-2- hexadecen-1-ol were found predominantly in the methanol extracts. </P><P> Conclusion: The leaf extract of P. odoratum showed potent anti-inflammatory and antioxidant activities, mediated by DLE and MLE, respectively.

scholarly journals Investigation of Total Polyphenol, Antioxidant Activity and Levels of Metals in Ethiopian Commercial Beers

2020 ◽  
Author(s):  
Molla Tefera ◽  
Daniel Ayele
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Average Concentration ◽  
Total Phenolic ◽  
Total Polyphenol ◽  
Flame Atomic Absorption ◽  
Flame Atomic Absorption Spectrophotometer ◽  
Metal Contents ◽  
Percent Recovery

Abstract Total polyphenol, antioxidant activity and heavy metal contents in selected Ethiopian beers (Dashen= B1, Harar = B2, Habesha= B3, Saint George= B4 and Walya= B5) were investigated. The average concentration of total phenolic content in B1, B2, B3, B4 and B5 were found to be 219.80, 206.09, 190.24, 193.00 and 212.10 mg GAE/L, respectively. The obtained results showed that B1 contained significantly highest total polyphenol content (219.80±2.83 mg/L) and showed the highest radical scavenging capacities (84.16±0.65). According to Pearson’s correlation between polyphenol extract with antioxidant activities, suggested that phenolic compound in beer were contributor to antioxidant activity. Similarly, all the beer samples were analyzed for Cd, Cr, Cu, Ni and Pb with flame atomic absorption spectrophotometer. The total metal concentrations of Cd, Cr, Cu Ni and Pb were found to be in the range of (ND–0.022 mg/L), (0.02 -0.041), (0.023-0.054), (ND-0.064) and (ND-0.022 mg/L), respectively. The efficiency of the procedure was validated by spiking and the percent recovery of the metals varied from 90.9-104.3%.

scholarly journals DPPH Scavenging Activities and Phytochemical Content of Four Asteraceae Plants

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Raden Bayu Indradi ◽  
Irda Fidrianny ◽  
Komar Ruslan Wirasutisna
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Dpph Method

Free radicals are atoms or molecules that have one or more unpaired electrons on its outer orbital, highly reactive, and could damage cell inside human body. Human body produce antioxidant to neutralize free radicals, but human ageing and stress oxidative conditions would increase the formation of free radicals, therefore an exogenous antioxidant are needed. Asteraceae family is the largest family among the plant kingdom therefore it has great potential as source of exogeneous antioxidants. The objectives of this research were to determine antioxidant activities of aerial part of elephant’s foot (Elephantopus scaber L.), false daisy (Eclipta alba (L.) Hassk.), Indian pluchea (Pluchea indica (L.) Less), and dandelion (Taraxacum officinale Weber ex F.H Wigg.) using DPPH method, determine total flavonoid and total phenolic content, and analyze correlation between total flavonoid content and total phenolic content with antioxidant activity. Extraction was carried out by reflux with increasing polarity using n-hexane, ethyl acetate, and ethanol respectively. Antioxidant activity was determined by DPPH method. Total flavonoid content was determined using Chang’s method and total phenolic content evaluated using Folin—Ciocalteu reagent. Correlation of total flavonoid content and total phenolic content was analyzed by Pearson’s method. Ethanolic extract of Indian pluchea showed the highest antioxidant activity with IC50 DPPH 16.66 ± 0.08 µg/mL. The highest total phenolic content (23.49 ± 0,56 g QE (Quercetin Equivalent)/100 g) was given by ethyl acetate extract of Indian pluchea, while the highest flavonoid content (16.48 ± 0.25 g GAE (Gallic Acid Equivalent)/100 g) was showed by ethanolic extract of Indian pluchea. Total phenolic content of elephant’s foot, false daisy and Indian pluchea herbs extracts showed significantly negative correlation with their IC50 of DPPH scavenging activities. Indian pluchea herbs extract had the highest antioxidant activity using DPPH method compared to elephant’s foot, false daisy and dandelion herbs. Phenolic compounds were the major contributor in antioxidant activities of elephant’s foot, false daisy and dandelion herbs extracts by DPPH method.

scholarly journals Effects of Various Drying Methods on Selected Physical and Antioxidant Properties of Extracts from Moringa oliefera Leaf Waste

2020 ◽  
Vol 12 (20) ◽  
pp. 8586
Author(s):  
Ade Chandra Iwansyah ◽  
Tran Dinh Manh ◽  
Yusuf Andriana ◽  
Muhammad Aiman bin Hessan ◽  
Faridah Kormin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Physical Properties ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Drying Methods ◽  
Sun Drying ◽  
Phenolic Contents ◽  
Tray Drying

This study aims to evaluate the effects of different drying methods on the physical properties, total phenolic content, total flavonoid content, and antioxidant activity of extracts from Moringa oliefera L. (MO) leaf waste. The effects of two drying techniques, namely, sun drying (A1) and tray drying (A2), on the physical and antioxidant properties of the extracts obtained using three extracting solvents, i.e., water (b1), ethanol (b2), and ethyl acetate (b3), were investigated. These extracts were analyzed for their physicochemical and antioxidant properties. The antioxidant properties were determined with the Folin Ciocalteau, aluminum chloride, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Fourier transform infrared spectrophotometry (FTIR) was used to identify functional groups in the active compounds. It was found that the physical properties of the MO extract, including yield, pH, total solids, and color, showed significant differences for the two drying methods (p < 0.05). The b3 extract had the highest value for total phenolic contents, total flavonoid contents, and antioxidant activities, followed by b2 and b1, respectively, for both the sun drying and tray drying methods. There was a significant correlation between the total phenolic and flavonoid contents and antioxidant activity (IC50). This study reveals that waste material from MO leaves could be utilized as an antioxidant agent, which is expected to reduce environmental pollution from the MO processing industry.

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