247 Metabolic Adaptation to Short-term of Dietary Nutrient Imbalances in Mid Lactation Dairy Cows

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 130-130
Author(s):  
Josef J Gross ◽  
Remo Stürmlin ◽  
Rupert M Bruckmaier
Keyword(s):  
Milk Yield ◽  
Feed Intake ◽  
Milk Fat ◽  
Mixed Model ◽  
Nitrogen Sources ◽  
Glucagon Secretion ◽  
Metabolic Adaptation ◽  
Short Term ◽  
Blood Concentrations ◽  
Plasma Urea

Abstract This study investigated the short-term metabolic and endocrine responses to different levels of concentrate formulated to particularly provide one major macronutrient source. Based on parity number, lactational stage, milk yield and BW, 9 mid lactating cows (211 ± 19 d in milk) were grouped into 3 blocks of 3 animals each. Concentrate supplements (aminogenic: rich in crude protein and nitrogen sources; glucogenic: high content of carbohydrates and glucogenic precursors; lipogenic: high lipid content) were fed in a factorial arrangement at 3 increasing levels for 3 d each. Milk yield, BW and feed intake were recorded daily. Blood and milk were sampled every 3 d at the end of each concentrate level. Statistical analysis was carried out using a mixed model with concentrate supplement and level as fixed, and block and animal as random factors. Milk fat, protein, lactose and urea contents were determined. In blood, concentrations of various metabolites, endocrine factors and enzyme activities (e.g. glucose, non-esterified fatty acids (NEFA), β-hydroxybutyrate, urea, cholesterol, triglycerides, insulin, glucagon, ASAT, GGT, GLDH) were measured. Milk yield, milk composition and BW were not affected by diets and concentrate levels. Feed intake increased in cows receiving the aminogenic and lipogenic diet. Milk und plasma urea concentrations were clearly elevated in the aminogenic and to a lesser extent in the lipogenic treatment compared to the glycogenic diet (P < 0.05). Glucose concentrations in plasma were not affected by treatments, whereas insulin and glucagon increased only in the aminogenic treatment with concomitantly reduced NEFA concentrations (P < 0.05). Activities of ASAT, GGT and GLDH as well as the total antioxidant capacity were not affected by diets (P >0.05). To conclude, immediate metabolic and endocrine responses occurred due to the short-term dietary changes. Particularly a surplus of nitrogen supply affected metabolic responses and stimulated insulin and glucagon secretion.

EFFECTS OF FEEDING A HIGH LEVEL OF TOWER RAPESEED MEAL IN DAIRY RATIONS ON FEED INTAKE AND MILK PRODUCTION

1977 ◽  
Vol 57 (4) ◽  
pp. 653-662 ◽  
Author(s):  
H. R. SHARMA ◽  
J. R. INGALLS ◽  
J. A. MCKIRDY
Keyword(s):  
Adverse Effect ◽  
Milk Production ◽  
Milk Yield ◽  
Feed Intake ◽  
Milk Fat ◽  
Rapeseed Meal ◽  
Feed Consumption ◽  
Short Term ◽  
N Retention ◽  
High Level

In experiment 1, 12 cows were used to compare the two (0–0) rapeseed meal (1788 and Tower) varieties with the commercial rapeseed meal (CRSM) and soybean meal (SBM). Feed intake, milk yield and fat content were not different (P > 0.05) among the four treatments; however, protein content was higher (P < 0.05) for the cows fed CRSM and SBM diets than for those fed the 1788–RSM diet. But more (P < 0.05) milk fat was produced by the cows fed 1788–RSM than by those fed CRSM and SBM diets. In experiment 2, eight cows were used to determine the effects of replacing SBM with Tower and also replacing a portion of Tower with urea (TU) in a mixed or extruded (TUE) form on feed intake, milk yield and nitrogen (N) retention. No differences were observed in feed consumption, milk yield or composition among the treatments. Serum thyroxine (T4) level was higher (P < 0.05) for the cows fed SBM than for those fed the 1788–SBM and was similar to levels for cows fed CRSM and Tower in the first experiment. However, no differences were found in thyroxine level in the second experiment. Extrusion of Tower–urea mixture increased (P < 0.05) the N retention compared with other treatments. These short-term studies suggest that up to 25% Tower RSM can be used in dairy rations without adverse effect on performance.

scholarly journals Characterization of Short-Term Heat Stress in Holstein Dairy Cows Using Altered Indicators of Metabolomics, Blood Parameters, Milk MicroRNA-216 and Characteristics

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 722
Author(s):  
Jang-Hoon Jo ◽  
Jalil Ghassemi Nejad ◽  
Dong-Qiao Peng ◽  
Hye-Ran Kim ◽  
Sang-Ho Kim ◽  
...  
Keyword(s):  
Heat Stress ◽  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Blood Parameters ◽  
Negative Effects ◽  
Short Term ◽  
Lactating Cows ◽  
Humidity Index ◽  
Holstein Dairy Cows

This study aims to characterize the influence of short-term heat stress (HS; 4 day) in early lactating Holstein dairy cows, in terms of triggering blood metabolomics and parameters, milk yield and composition, and milk microRNA expression. Eight cows (milk yield = 30 ± 1.5 kg/day, parity = 1.09 ± 0.05) were homogeneously housed in environmentally controlled chambers, assigned into two groups with respect to the temperature humidity index (THI) at two distinct levels: approximately ~71 (low-temperature, low-humidity; LTLH) and ~86 (high-temperature, high-humidity; HTHH). Average feed intake (FI) dropped about 10 kg in the HTHH group, compared with the LTLH group (p = 0.001), whereas water intake was only numerically higher (p = 0.183) in the HTHH group than in the LTLH group. Physiological parameters, including rectal temperature (p = 0.001) and heart rate (p = 0.038), were significantly higher in the HTHH group than in the LTLH group. Plasma cortisol and haptoglobin were higher (p < 0.05) in the HTHH group, compared to the LTLH group. Milk yield, milk fat yield, 3.5% fat-corrected milk (FCM), and energy-corrected milk (ECM) were lower (p < 0.05) in the HTHH group than in the LTLH group. Higher relative expression of milk miRNA-216 was observed in the HTHH group (p < 0.05). Valine, isoleucine, methionine, phenylalanine, tyrosine, tryptophan, lactic acid, 3-phenylpropionic acid, 1,5-anhydro-D-sorbitol, myo-inositol, and urea were decreased (p < 0.05). These results suggest that early lactating cows are more vulnerable to short-term (4 day) high THI levels—that is, HTHH conditions—compared with LTLH, considering the enormous negative effects observed in measured blood metabolomics and parameters, milk yield and compositions, and milk miRNA-216 expression.

scholarly journals Structural and non-structural carbohydrates in concentrate supplements of silage-based dairy cow rations. 1. Feed intake and milk production.

1990 ◽  
Vol 38 (3B) ◽  
pp. 487-498
Author(s):  
H. de Visser ◽  
P.L. van der Togt ◽  
S. Tamminga
Keyword(s):  
Protein Content ◽  
Milk Yield ◽  
Feed Intake ◽  
Milk Fat ◽  
Milk Protein ◽  
Basal Diet ◽  
Negative Energy ◽  
Net Energy ◽  
Rumen Degradation ◽  
Silage Maize

A feeding trial was carried out with 64 multiparous dairy cows, in which the effect of type of carbohydrate in concentrate mixtures (starch vs. cell wall constituents) and differences in rumen degradation (fast vs. slow) on feed intake and milk yield were studied. The experiment started immediately after parturition and lasted for 15 wk. The basal diet, which comprised 75% of the total DM intake, consisted of wilted grass silage, maize silage and concentrates. The remaining part of the diet consisted of barley (B), maize (M), pressed ensiled beet pulp (P) or moist ensiled maize bran (MB). All diets were given as totally mixed rations. Total intake of DM and net energy did not differ between diets, but differences were found in energy partition. There was a tendency for cows given diet B to show increased liveweight gain, while cows given P mobilized more body reserves compared with the other treatments. Milk yield did not differ between diets, but milk fat content was higher for diet P. Milk protein content was higher for diets B and M compared with P and MB. The lower protein content of the milk of treatment P can be explained by a longer period of negative energy balance, while the lower milk protein in cows given diet MB probably resulted from reduced microbial protein synthesis. (Abstract retrieved from CAB Abstracts by CABI’s permission)

Effect of feeding hay vs. silages of various types to dairy cows on feed intake, milk composition and coagulation properties

2020 ◽  
Vol 87 (3) ◽  
pp. 334-340
Author(s):  
Elisa Manzocchi ◽  
Werner Hengartner ◽  
Michael Kreuzer ◽  
Katrin Giller
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Feed Intake ◽  
Milk Fat ◽  
Fixed Effects ◽  
Experimental Period ◽  
Milk Composition ◽  
Processing Technology ◽  
Maize Silage ◽  
Rennet Coagulation

AbstractThis research paper addresses the hypotheses (1) that milk produced from hay-fed cows differs from that of silage-fed cows and (2) that silage type has an important impact, too. Four diets differing in forage type but with equal estimated milk production potential and a forage:concentrate ratio of 0.85 : 0.15 were compared regarding their effect on feed intake, milk yield and milk properties. The forages tested were hay, grass silage, conventional short-chopped and long-chopped maize silage subjected to a novel processing technology (Shredlage®). Twenty-four dairy cows were fed two of the four diets in two consecutive runs in an incomplete (4 × 2) Latin-square design (n = 12 per diet). Each experimental period lasted 22 d, with 12 d of adaptation and 10 d of sampling. During sampling, feed intake and milk yield were recorded daily, milk composition and coagulation properties were determined four times. The composition of the diet ingredients was analysed weekly. Data were analysed with a mixed model considering feed, period and their interaction as fixed effects. Stage of lactation, milk yield and milk composition from the pre-experimental period were used as covariates in the model. Dry matter intake was lower with the long-chopped processed maize silage compared to the other three groups. There were some diet differences in intakes of net energy for lactation and absorbable protein in the duodenum, but this did not result in changes in milk yield. The milk fat content was higher with the grassland-based diets compared to the maize silage diets. No treatment effect on milk acidity and rennet coagulation properties was observed. In conclusion, there were no indications for specific physico-chemical properties of milk from a hay-based diet, and maize processing technology was not of large effect either. Future investigations should focus on sensory differentiation of the milk produced with different forages.

scholarly journals Phenotypic Characterization of Milk Yield and Quality Traits in a Large Population of Water Buffaloes

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 327 ◽  
Author(s):  
Angela Costa ◽  
Riccardo Negrini ◽  
Massimo De Marchi ◽  
Giuseppe Campanile ◽  
Gianluca Neglia
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Large Scale ◽  
Mixed Model ◽  
Large Population ◽  
Solid Content ◽  
Current Status ◽  
Phenotypic Characterization ◽  
Italian Population ◽  
Milk Traits

The buffalo milk industry has economic and social relevance in Italy, as linked to the manufacture of traditional dairy products. To provide an overview of the current status of buffaloes’ performances on a large scale, almost 1 million milk test-day records from 72,294 buffaloes were available to investigate milk yield, energy corrected milk, fat, protein, and lactose content, and somatic cell score (SCS). Phenotypic correlations between milk traits were calculated and analysis of variance was carried out through a mixed model approach including fixed effect of parity, stage of lactation, sampling time, month of calving, and all their interactions and random effects of buffalo, herd-test-date, and residual. Third-parity buffaloes were the most productive in terms of milk yield, while the lowest solid content was detected in sixth parity buffaloes. A considerable gap between primiparous and multiparous buffaloes was observed for milk yield, especially in early- and mid-lactation. Overall, SCS progressively increased with parity and showed a negative correlation with milk yield in both primiparous (−0.12) and multiparous (−0.14) buffaloes. Results suggested that, at the industrial level, milk of primiparous buffaloes may be preferred for transformation purposes, since it was characterized by greater solid content and lower SCS. Results of this study provide a picture of the Italian population of buffaloes under systematic performance records and might be beneficial to both dairy industry and breeding organizations.

Effect of intravenous bovine growth hormone or human pancreatic growth hormone-releasing factor on milk production and plasma hormones and metabolites in sheep

1985 ◽  
Vol 105 (2) ◽  
pp. 189-196 ◽  
Author(s):  
I. C. Hart ◽  
P. M. E. Chadwick ◽  
S. James ◽  
A. D. Simmonds
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Milk Yield ◽  
Milk Fat ◽  
Latin Square ◽  
Plasma Urea ◽  
Gh Secretion ◽  
Vehicle Treatment ◽  
Plasma Hormones ◽  
Plasma Gh

ABSTRACT Although it is well known that exogenous bovine GH (bGH) increases milk yield in ruminants it has not been possible to determine whether an increase in endogenous GH secretion has the same effect. The recent isolation of human pancreatic GH-releasing factor (hpGRF-44) has enabled this comparison of the effects of bGH and hpGRF-44 on milk production in sheep. Three pairs of Dorset ewes underwent three 4-day treatments according to a Latin square design. Treatment 1 involved: 2-hourly i.v. injections (∼ 3·0 ml) of bGH (15 μg/kg; 1·8 units/mg); treatment 2: 2-hourly i.v. injections (∼3·0 ml) of hpGRF-44 (0·6 μg/kg); treatment 3: 2-hourly i.v. injections (3·0 ml) of the vehicle. Treatment periods were separated by 10 days. Sheep were milked twice daily and the milk was analysed for fat, protein and lactose. Blood samples (5·0 ml) were taken before and at 15, 45, 75 and 100 min after every third injection throughout the 4 days. Plasma was analysed for insulin, glucose, urea and non-esterified fatty acids (NEFA). The changes in plasma GH stimulated by hpGRF-44 were consistent and repeatable throughout the 4 days of treatment. In comparison to the controls, treatment with hpGRF-44 and bGH significantly increased average plasma GH (μg/l) for the 4 days (control, 5·7±0·2 (s.e.m.); hpGRF-44, 12·3±0·4, P<0·001; bGH, 14·5±0·5, P<0·001), and this was accompanied by similar increases in milk yield (hpGRF-44, 26·7±2·6%, P<0·001; bGH, 30·7±2·9%, P<0·001) and the concentration of milk fat (g/kg) (control, 28·6±0·5; hpGRF-44, 33·2±0·5; bGH 34·8±1·5, P<0·01 for both treatments). The post-treatment decline in milk yield was much slower after hpGRF-44 than after bGH injections. Both treatments stimulated significant increases in the average concentrations of plasma insulin, glucose and NEFA, and plasma urea was significantly reduced by treatment with bGH only. It is concluded that raising endogenous GH secretion is equally as effective as exogenous bGH in stimulating milk production in sheep. J. Endocr. (1985) 105, 189–196

scholarly journals Effect of different levels of supplied cobalt on the fatty acid composition of bovine milk

2012 ◽  
Vol 109 (5) ◽  
pp. 834-843 ◽  
Author(s):  
Inger J. Karlengen ◽  
Ole Taugbøl ◽  
Brit Salbu ◽  
Are H. Aastveit ◽  
Odd M. Harstad
Keyword(s):  
Milk Yield ◽  
Feed Intake ◽  
Milk Fat ◽  
Bovine Milk ◽  
Latin Square ◽  
Basal Diet ◽  
Cobalt Acetate ◽  
Linear Effect ◽  
Fa Composition ◽  
Four Levels

In previous studies, administration of high amounts of Co decreased the proportion of MUFA in bovine milk. The present study was conducted to examine the amount of Co needed to obtain this effect. High-yielding dairy cows (n4), equipped with ruminal cannulas, were used in a 4 × 4 Latin square design study. The basal diet consisted of concentrate mixture (9 kg/d) without added Co and grass silage (ad libitum). The following four levels of Co were administrated as cobalt acetate dissolved in distilled water: no Co (treatment 1, T1); 4·0 mg Co/d (T2); 380 mg Co/d (T3); 5300 mg Co/d (T4). Each period lasted for 18 d, including 11 d of treatment. During the treatment periods, the solutions were continuously infused into the rumen. Milk yield and milk concentration of fat, fatty acids (FA), protein, lactose, Co, Zn, Fe and Cu were determined. Blood plasma was analysed with respect to FA, Co, Zn, Fe and Cu. Feed intake and total tract digestibility of feed components were also determined. There was a linear effect of increasing the level of Co on milk FA composition. The effects of Co on FA composition in blood were insignificant compared with the effects on milk. In milk fat, the concentration ofcis-9-18 : 1 was reduced by as much as 38 % on T4 compared with T1. Feed intake and milk yield were negatively affected by increasing the Co level.

Short-term effects of level of dry matter intake upon milk yield, fatty acid composition of milk fat, and some blood constituents of Hereford cows

1976 ◽  
Vol 27 (1) ◽  
pp. 175 ◽  
Author(s):  
TH Stobbs ◽  
DJ Brett
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Composition ◽  
Milk Yield ◽  
Milk Fat ◽  
Dry Matter ◽  
Reliable Index ◽  
Short Term ◽  
Calf Growth

The effect of two levels of feed intake (lucerne hay with mean digestible organic matter intakes (DOMI) of 6.1 and 9.1 kg/animal/day) on milk yield, calf growth rate, fatty acid composition of milk fat, and blood metabolites (non-esterified fatty acids and glucose) of suckled Hereford cows in their second and third month of lactation was measured to determine the most accurate indicators of intake of nutrients. Milk yields of cows averaged 5.0 and 6.1 kg/day on low and high intakes respectively, but production was extremely variable and poorly correlated with DOMI (r = 0.33). Calf growth rates were correlated with the milk yield of dams (r = 0.41) and with DOMI (r = 0.37). The fatty acid composition of milk fat provided the most repeatable (coefficient of variation of 6%) and reliable index of the feed intake, correlations being 0.71 with C4–C16 acids and –0.65 with oleic acid. Dry matter restriction decreased the proportion of C4–C16 acids (68.5% to 62.3%), while the proportion of oleic acid increased (17.0% to 21.9%). Cows responded rapidly to the level of feeding imposed, and no residual effects of previous feeding treatments were measured after 14 days. Non-esterified fatty acid levels in blood increased with restriction of feed (244–377 µ-equiv.11) and were poorly correlated (r = –0.16) with the intake, while the glucose content did not vary (mean of 70.6 mg/100 ml). It was concluded that the fatty acid composition of milk fat provides a reliable index of variation in the level of nutrient intake by suckled beef cows and could be used in short-term grazing studies.

scholarly journals Nutritional evaluation of ensilage made from intercropped corn and sunflowers

1993 ◽  
Vol 73 (3) ◽  
pp. 539-545 ◽  
Author(s):  
L. J. Fisher ◽  
S. Bittman ◽  
Z. Mir ◽  
P. Mir ◽  
J. A. Shelford
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Volatile Fatty Acids ◽  
Negative Impact ◽  
Milk Composition ◽  
Corn Silage ◽  
Plasma Metabolites ◽  
Plasma Urea ◽  
Lactating Cows ◽  
Rumen Ph

The feeding value of sunflower–corn silage relative to that of corn silage as the only forage fed lactating cows was established using 12 lactating Holstein cows in an experiment with a 2 × 2 Latin square design. Treatment periods were 5 wk and there was no changeover period between treatments. A constant grain intake to milk yield ratio of 1:4 was maintained. The comparison was based on dry matter (DM) intake, milk yield and composition, body-weight change, rumen pH and molar proportions of volatile fatty acids and plasma metabolites. The sunflower–corn silage was lower in DM content (25.4 vs. 29.2%), but higher in protein (11.5 vs. 8.0%), acid detergent fiber (33.2 vs. 29.2%), fat (8.1 vs. 2.1%) and total ash (10.6 vs. 4.9%). Silage DM intake was the same for both silages, but milk yield was significantly greater (P < 0.05) (32.7 vs. 31.2 kg d−1) for cows fed sunflower–corn silage. However, both milk fat (3.19 vs. 3.87%) and milk protein (3.04 vs. 3.26%) were lower (P < 0.05) for cows fed sunflower–corn silage, resulting in a higher (P < 0.05) fat-corrected milk yield (30.5 vs. 28.4 kg d−1) for the cows fed corn silage. Type of silage fed did not alter rumen pH, but the molar percentage of acetic acid was higher (P < 0.05) and the molar percentages of isovaleric and valeric acids were lower when sunflower–corn silage was fed. The feeding of sunflower–corn silage resulted in a significant increase (P < 0.05) in plasma urea nitrogen (10.60 vs. 7.53 mg 100−1), cholesterol (7.36 vs. 5.63 mmol L−1) and triglyceride (0.20 vs. 0.18 mmol L−1). Plasma levels of methionine (29.9 vs. 18.5 mmol mL−1) and valine (208.0 vs. 191.0 N mol mL−1) were greater for cows fed the sunflower–corn silage. It was concluded from this study that intercropped sunflower–corn harvested and preserved as silage is an acceptable source of forage for lactating cows, but when fed as the only source of forage it may have a negative impact on milk composition. Key words: lactating cows, sunflower–corn silage, milk yield, milk composition

An energy-protein feed additive containing different sources of fat improves feed intake and milk performance of dairy cows in mid-lactation

2019 ◽  
Vol 86 (1) ◽  
pp. 55-62
Author(s):  
Piotr Micek ◽  
Zygmunt M. Kowalski ◽  
Marek Sady ◽  
Jolanta Oprządek ◽  
Jacek Domagała ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Yield ◽  
Feed Intake ◽  
Milk Fat ◽  
Functional Health ◽  
Feed Additive ◽  
Nutritional Effect ◽  
Total Fatty Acids ◽  
Protein Feed

AbstractThis research paper addresses the hypothesis that calcium salts combined with whole linseed and heat-treated rapeseed cake in one feed additive may efficiently stimulate the productivity of dairy cows and have a positive effect on the functional (health-promoting) properties of milk fat. The article proposes the composition of such an additive (EFA) and evaluates its nutritional effect in the diet of mid-lactation dairy cows. Forty multiparous Polish Holstein-Friesian (PHF) dairy cows were allocated to one of four treatments (10 cows/treatment) and fed a TMR diet without EFA or with EFA in the amount of 1, 2 or 3 kg/d per head for a 63-d-period. Individual intake of dry matter (DMI) and nutrients was determined, as was milk yield and composition, including fatty acid profile, fat soluble vitamins, cholesterol and phospholipids (PLs). Irrespective of the treatment group, cows fed diets with EFA had higher (P < 0.05) DMI, milk yield and milk vitamin D3 and K2 concentration but lower (P < 0.01) milk protein, fat and cholesterol contents. The additive did not affect the milk concentrations of β-carotene or vitamin A or E. The PLs content was correlated with fat concentration in the milk and decreased as the level of EFA in the diet increased. An increase in phosphatidylcholine in total PLs was accompanied by a reduction in the proportion of sphingomyelin (P < 0.05). The use of EFA increased the proportion of polyunsaturated fatty acids (PUFA) in the total fatty acids in the milk. The addition of EFA in the amount of 3 kg increased the proportion of PUFA by 77% (P < 0.05). In conclusion, the use of an energy-protein feed additive (EFA) increases feed intake and milk yield in cows and alters milk fat composition, improving its functional properties. Higher milk production compensates for the decrease in solids concentration in the milk, which has no effect on their daily yield.

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