scholarly journals Diurnal and dietary impacts on estimating microbial protein flow from urinary purine derivative excretion in beef cattle1

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Grant I Crawford ◽  
Jim C MacDonald ◽  
Andrea K Watson ◽  
Galen E Erickson ◽  
Terry J Klopfenstein

Abstract Two experiments were conducted to determine the effects of diet composition and time of urine spot sampling on estimates of urinary purine derivative (PD) excretion. In Exp. 1, 116 individually fed crossbred heifers (407 ± 32 kg) were arranged in a randomized block design (82 d). Treatments were arranged in a 2 × 3 factorial design, with two urine spot sample collection times (0700 and 1700 hours; AM and PM) and three diets: 85% steam-flaked corn (SFC); 85% SFC + 1.5% urea (UREA); or 25% SFC, 30% wet corn gluten feed, and 30% corn bran (BYPROD). In Exp. 2, six ruminally and duodenally fistulated steers (474 ± 37 kg) were arranged in a replicated 3 × 3 Latin square design, with dietary treatments identical to Exp. 1 (63 d). Treatment diets were selected to result in varied amounts of microbial crude protein (MCP) in order to evaluate the accuracy of using estimates of urinary PD excretion to predict MCP. Urine spot samples were collected at 0700, 1200, 1700, and 2200 hours. No urine collection time × diet interactions occurred (P > 0.20) for any variable in either experiment. In Exp. 1, dry matter intake (DMI) was greatest with BYRPOD (10.40 kg/d) and lowest with SFC (7.90 kg/d; P < 0.05). Feed efficiencies were greatest for UREA (0.182) and least for SFC (0.141; P < 0.05). Urinary PD:creatinine (PD:C) ratio was greatest for BYPROD (1.25) and least for SFC (0.94; P < 0.05). Urine spot sampling time had a significant (P < 0.05) impact on PD:C, 1.03 for AM and 1.22 for PM samples. In Exp. 2, DMI was greater (P < 0.05) with BYPROD than with SFC and tended (P = 0.07) to be greater with BYPROD than with UREA. Ruminal pH was greatest for BYPROD (5.94; P < 0.05). Flow of MCP was 636, 829, and 1,056 g/d for SFC, UREA, and BYPROD, with BYPROD being greater (P < 0.05) than SFC and tending (P = 0.06) to be greater than UREA. Urinary PD:C was greater (P < 0.05) for BYPROD than SFC and tended (P = 0.09) to be greater for UREA than SFC. Urinary PD:C increased linearly (P < 0.05) with sampling time. Diets formulated to affect DMI and MCP flow resulted in differences in urinary PD excretion, and these results related well with MCP flow estimated from duodenal purines. Collecting spot samples of urine later in the day resulted in greater estimates of urinary PD excretion; purine and PD flows appear to increase with time after one morning feeding per day. This method is well suited to evaluating relative differences between treatments but should not be extrapolated to assume absolute values.

1970 ◽  
Vol 37 (1) ◽  
pp. 10-16
Author(s):  
S Barua ◽  
MJ Khan ◽  
AKFH Bhuiyan ◽  
MN Islam ◽  
SS Islam

The study was undertaken to investigate the effects of concentrate supplementation with different protein levels on intake, digestibility and growth performance of Red Chittagong (RC) heifers fed urea molasses straw (UMS) based diet. Twelve RC heifers having average live weight of 124.83±43.15 kg and aged between 8 to 14 months were selected for 90 days feeding trial. Animals were divided into four groups having three animals in each and were randomly assigned to four dietary treatments in a Randomized Block Design (RBD). The experimental diets were formulated using urea molasses straw (UMS), German grass (Echinochloa grousgalli) with or without concentrate mix (having different levels of protein). All the animals received UMS ad libitum and German grass at the rate of 20% of total DM intake. In addition to UMS and German grass of control diet T0, animals on diets T1, T2 and T3 were supplied with concentrate mixture at the rate of 10% of DM intake containing 15, 20 and 25% CP, respectively. Average daily DM intake was 2.65, 3.06, 2.62 and 2.86 kg/100 kg LW for diets T0, T1, T2 and T3, respectively and the difference was non significant (P>0.05). The digestibility of DM, CP, EE, NFE and digestible nutrients (DCP, DEE and DNFE) for diets T1, T2 and T3 was significantly higher than those for diet T0. The daily average liveweight gain of RC heifers fed diets T0, T1, T2 and T3 were 100, 275, 333 and 291 g respectively (P<0.01). Concentrate mixture having 20% crude protein may be supplemented at the rate of 10% of DM intake per day for optimum growth of Red Chittagong heifers. DOI: http://dx.doi.org/10.3329/bjas.v37i1.9861 BJAS 2008; 37(1): 10-16


1996 ◽  
Vol 76 (2) ◽  
pp. 271-273 ◽  
Author(s):  
T. A. Van Lunen ◽  
H. Schulze

A trial was conducted to evaluate the effects of supplementing wheat- and corn-based diets with xylanase on growth performance and FCR of pigs from 10 to 18 wk of age. Seventy-tow castrated male pigs were assigned to pens of two and in a randomized block design to six dietary treatments consisting of diets containing 60% wheat, 40% wheat and 20% corn, and 20% wheat and 40% corn with and without supplementation with xylanase. Feed and water were available ad libitum. Xylanase supplementation improved growth rate and FCR by 9.2 and 5.3%, respectively, regardless of level of wheat and corn inclusion. Key words: Pig, growth, feed enzyme, Trichoderma longibrachiatum xylanase, wheat, corn


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 345-346
Author(s):  
Jose Alvear ◽  
Jorge Ron ◽  
Hector Torrealba ◽  
Christian H Ponce

Abstract An experiment was conducted to evaluate the effects of corn protein concentrate (CPC) levels on performance, nitrogen digestibility, blood parameters, and visceral organ mass by newly weaned piglets. Forty piglets (6. 9 kg ± 1.2 kg of live weight) were allotted to 4 dietary treatments in a completely randomized block design. Piglets were fed individually 1 of 4 experimental diets containing different CPC levels (0%, 5%, 10% or 15%) during 44 d. Performance parameters were measured weekly. Total fecal and urine samples were collected on d 33 of the experiment for 4 consecutive days to determine N balance. At the end of the feeding period, blood samples were collected to measure blood parameters, and animals were slaughter to determine visceral organ mass. Data were analyzed as a randomized complete block designed using PROC MIXED in SAS. Final BW tended to decrease linearly (P = 0.065) as dietary CPC level increased. Overall, ADG tended to decrease linearly (P = 0.065), and ADFI was significantly reduced (P = 0. 038) as dietary CPC level increased. Nevertheless, FCR was not by dietary treatment (P = 0.1872). There were no differences on nitrogen intake, retention and digestibility across dietary treatments (P &gt; 0. 407). However, there was a tendency to decrease linearly N excretion on urine as CPC increased in the diet. Hematocrit and Serum Urea-N were linearly increased and reduced, respectively as CPC increased in the diet (P &lt; 0. 027). Empty BW at d 44 tended to linearly reduce as CPC increased. Pancreas weight was reduced by inclusion of CPC (linearly; P = 0. 005). In summary, it was demonstrated that, when CPC replaced partially traditional protein sources up to 5% of the diet, performance and N status of the animal are not altered. However, caution should be taken with higher levels of inclusion.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 25-25
Author(s):  
Andrea L Warner ◽  
Paul A Beck ◽  
Andrew P Foote ◽  
Colton Robison ◽  
Kaitlyn Pierce ◽  
...  

Abstract Increased cotton production in the Southwest U.S. has increased the availability of cotton byproducts for use in cattle diets. The objective of this experiment was to evaluate the inclusion of cotton byproducts in feedlot finishing rations on the performance, carcass characteristics, and fecal characteristics of steers. Crossbred beef steers (n = 64; BW = 318 ± 12.3 kg) were assigned to 1 of 2 experimental treatments in a randomized complete block design (8 pens/treatment; 4 steers/pen). Treatments included a control (CON) diet (7% hay, 15% wet corn gluten feed, 67% rolled corn, 5% liquid supplement) and a cotton byproduct (CTN) diet (7% gin trash, 15% whole cottonseed, 72% rolled corn, 5% water). Both diets included 0.75% urea and 5% dry supplement. The CTN steers tended to have a heavier final BW (P = 0.09) and greater ADG (P = 0.08). Over the entire feeding period, DMI was greater for CTN steers than CON steers (P = 0.04) with no difference in G:F between treatments (P = 0.89). The CTN steers had heavier HCW (P = 0.02) and greater fat thickness (P = 0.03) than CON steers, but marbling score and REA were not different between treatments (P ≥ 0.64). Steers fed the CON diet tended to have a lower YG (P = 0.07), less KPH fat (P = 0.09), and lower dressing percentage (P = 0.10) than CTN steers. Liver scores did not differ between treatments (P ≥ 0.17). Fecal scores were lower for CTN steers on d 56 (P = 0.03) and fecal pH tended to be higher for the CTN steers on d 28 (P = 0.09), but neither differed during other periods (P ≥ 0.18). These results suggest that cotton byproducts can be used in feedlot rations without adverse effects on performance or carcass characteristics.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 398-399
Author(s):  
Sinthia Siqueira ◽  
Hébert De Brito ◽  
Wagner Araújo ◽  
Hélio Neves ◽  
Mara Benfato ◽  
...  

Abstract The present study aimed to evaluate the impact of a mycotoxin deactivator in diets containing added mycotoxins for gestating and lactating gilts on their performance. A total of 60 gilts (LD x LW) were used. After insemination the gilts were distributed among 3 dietary treatments according to a randomized block design: a negative control (CON); mycotoxin positive control (Myc); and a positive control added a mycotoxin deactivator (Myc+Deact 2 kg/ton; Unike®Plus, Adisseo). The total mycotoxins levels added to the diets 2 and 3 from d 75 of gestation until farrowing were: DON at 2.5 mg/kg, FBs at 10 mg/kg, and ZEA at 0.75mg/kg. Sows received the experimental diets from d 75 of gestation until weaning (i.e. 21 d). At farrowing, total number of piglets born, born alive, stillborn, and mummies were measured. Piglets were individually weighed 24-h post-farrowing, and at weaning to determine litter performance during lactation. The effects of diet composition, blocks and initial weight were tested according to a general linear procedure analysis of variance (GLM procedure of SAS). During gestation, treatments influenced (P = 0.022) sow mortality rate: CON had no mortality and Myc 15.8% and Myc+deact 10.5%. Same observation was made for the lactation period (P = 0.017): CON showed a lower mortality rate compared to the mycotoxin fed sows (5.0 vs. 31.2 vs. 11.8%, respectively for CON, Myc and Myc+deact). Sow milk production yield was greater (P = 0.021) in CON (11.66 vs. 9.53 vs. 9.67 kg/ d, respectively for CON, Myc and Myc+deact). As a consequence of that, treatments also influenced piglet (P = 0.047) and litter (P = 0.048) weight with a higher value for CON sows, followed by Myc+deact and Myc. Our findings showed that the use of the deactivator can mitigate the negative effects on performance when animals are subjected to diets contaminated by challenging levels of mycotoxins.


2003 ◽  
Vol 83 (1) ◽  
pp. 101-104 ◽  
Author(s):  
P. A. Thacker ◽  
B. G. Rossnagel ◽  
V. Raboy

Twenty-four crossbred barrows (Camborough 15 Line female × Canabred sire) weighing an average of 51.3 ± 5.1 kg were used in a trial conducted during two periods according to a completely randomized block design. The pigs were assigned to one of six dietary treatments comprised of a normal two-rowed, hulled variety of barley (CDC Bold) or three low-phytate genotypes selected to contain approximately 68, 41 or 3% of the normal level of phytate. A normal hulless barley (CDC Dawn) and a hulless genotype selected to contain 54% of the normal phytate content were also included. The diets fed contained 99.5% barley and 0.5% chromic oxide. The marked feed was provided for a 7-d acclimatization period, followed by a 3-d fecal collection. At the conclusion of the first replicate, the pigs were assigned to different treatments and the procedure was repeated resulting in eight observations per treatment. Selection for phytate content had no effects on the apparent fecal digestibility of dry matter, crude protein or gross energy (P > 0.05). The digestibility coefficients for dry matter and energy were significantly higher for the hulless varieties of barley than the hulled genotypes. Phosphorus digestibility increased as the level of phytate in the barley declined. The linear relationship relating phosphorus digestibility (y) to phytate content (x) was: y = 67.7 − 12.54x       (r2= 0.64; P < 0.0001).


Author(s):  
Everton T. M. Ichikawa ◽  
Adalton M. Fernandes ◽  
Lydia Helena da S. de O. Mota

ABSTRACT The objective of this study was to evaluate the rooting and growth of sweet potato seedlings in trays in response to substrate supplementation with calcium (Ca) and phosphorus (P). Two greenhouse experiments were conducted in a randomized block design with a split plot scheme and eight repetitions. In the Ca experiment, the plots were the doses of 0, 100 and 200 mg kg-1 of Ca, and in the P experiment, by the doses of 0, 150 and 300 mg kg-1 of P. In both experiments, the subplots corresponded to the sampling time of seedlings (15, 30, 45, 60 and 75 days after planting). The Ca present in the substrate was sufficient to promote the proper rooting and growth of sweet potato seedlings in the trays. The Ca supply in excess (200 mg kg-1) adversely affected the growth of seedlings that remained in the tray for more than 60 days. Although the seedlings supplied with P showed higher root growth rate after 45 days, the initial P available in the substrate was sufficient to promote the adequate growth of the seedlings in the trays until 60 days.


2021 ◽  
Vol 41 (1) ◽  
pp. 100-110
Author(s):  
J. O. Jegede ◽  
T. S. B. Tegbe ◽  
S. O. Ogundipe ◽  
P. I. Rekwot ◽  
S. B. Afolayan

Twenty four crossbred (Large White X Hampshire) female weaner pigs of average initial weight of 12.6 kg live weight were raised for a period 126 days from weaner to growing-finishing stage of average weight of 71.0kg in a trial in which undelinted undecorticated cottonseed cake (UUCSC) was fed at 0, 10, 20 and 30% of the 18%% or 16% crude protein diets. Animals were randomly assigned to pens on the basis of initial weight group and there were two pigs per pen in a randomized block design trial. Animals were fed 5% body weight and had free access to drinking water Gilts were weighed weekly and the trial lasted for 126 days. Twelve of the gilts were randomly selected and slaughtered for carcass analysis. As a result of this study, slaughter and carcass weights of gilts fed 30% UUCSC diet were significantly higher (P<0.05) than those fed 0, 10 and 20% UUCSC diets. The prime cuts (ham, shoulder and loin) were similar across dietary treatments. There was significant linear (P<0.05) reduction in loin-eye area while the back fat thickness significantly (P<O.05) increased as the levels of UUCSC increased in the diets. The gilts fed 30% UUCSC had significantly (P<0.05) lower liver kidneys and spleen weights as compared to those fed 0, 10and 20% UUCSC diets, Between the lungs and hearts of the gilts there were no significant differences (P>0.05) among them. There was significant linear (P<O.05) increase in stomach dimension while the gut dimensions were similar across dietary treatments. For good carcass quality, 20% UUCSC can be fed to gilts


Author(s):  
Milton C. Padilha Junior ◽  
Sérgio L. R. Donato ◽  
Paulo E. R. Donato ◽  
João A. da Silva

ABSTRACT The objective of this study was to evaluate the chemical attributes of the soil cultivated with ‘Gigante’ cactus pear under different doses of organic fertilizer, planting spacings and sampling times. Four doses of organic fertilizer with bovine manure (0, 30, 60 and 90 Mg ha-1 year-1), three spacings (1.0 x 0.5, 2.0 x 0.25 and 3.0 x 1.0 x 0.25 m) and three sampling times (0, 390 and 600 days after planting) corresponding to the periods prior to planting, growth and harvest of the first cycle, were evaluated in a randomized block design in a 4 x 3 x 3 factorial scheme, with three repetitions. The pH in water, macronutrients (P, K, Ca, Mg), micronutrients (B, Cu, Fe, Mn, Zn), variables of the soil sorption complex (H + Al, SB, CEC, T, V) and soil organic matter (SOM) were determined. Organic fertilization increased the values of SOM, P, K, Ca, Mg, SB, CEC, T and V and reduced H + Al in soils cultivated with ‘Gigante’ cactus pear at different times. The doses of manure increased pH and Mn, regardless of sampling time and spacing. The spacings did not influence the chemical attributes of the soil.


2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Eudia Christina Wulandari ◽  
Angela Nitia Nefasa

The aim of the present research was to evaluate the contribution effect of feeding Wulandari et al. (2013) and Wulandari et al. (2015) rations and free feeding choice in Arab hen. One hundred birds of Arab hen, age was + 14 weeks, with an average body weight of 814,73 g ± 46,86 g were used as experimental animals, and were divided randomly into a randomized block design with 3 treatments and 6 replications. Feedstuffs used for dietary treatments were rice bran, yellow corn, fish meal, Azolla microphylla, CaCO3, oyster shell, meat bone meal, soybean meal, pollard, and top mix. The rations were formulated as ration from Wulandari et al. (2013) and Wulandari et al. (2015). At the same time, there are feedstuff which gived ad libitum without formulated. Conclusion of the research is that free feeding choice affect by palatability feedstuff and gives a random data. Consumption ration on free feeding choice more decrease than another treatments. Keywords: arab’s laying hans, free feeding choice, productivity


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