scholarly journals High-energy diet enhances spermatogenic function and increases sperm midpiece length in fallow deer ( Dama dama ) yearlings

2019 ◽  
Vol 6 (6) ◽  
pp. 181972 ◽  
Author(s):  
José Luis Ros-Santaella ◽  
Radim Kotrba ◽  
Eliana Pintus
Keyword(s):  
Sexual Development ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
High Energy ◽  
Fallow Deer ◽  
Barley Grain ◽  
Control Group ◽  
Testosterone Levels ◽  
Sperm Midpiece ◽  
Experimental Group

Nutrition is a major factor involved in the sexual development of livestock ruminants. In the male, a high-energy diet enhances the reproductive function, but its effects on the underlying processes such as spermatogenic efficiency are not yet defined. Moreover, the possible changes in sperm size due to a supplemented diet remain poorly investigated. The main goal of this study was to evaluate whether a high-energy diet affects the spermatogenic activity, epididymal sperm parameters (concentration, morphology, morphometry and acrosome integrity) and blood testosterone levels in fallow deer yearlings. For this purpose, 32 fallow deer were allocated into two groups according to their diet: control (pasture) and experimental (pasture and barley grain) groups. Fallow deer from the experimental group showed a significant increase in the Sertoli cell function and sperm midpiece length, together with a higher testicular mass, sperm concentration and percentage of normal spermatozoa than the control group ( p < 0.05). We also found a tendency for higher blood testosterone levels in the animals fed with barley grain ( p = 0.116). The better sperm quality found in the experimental group may be related to their higher efficiency of Sertoli cells and to an earlier onset of puberty. The results of the present work elucidate the mechanisms by which dietary supplementation enhances the male sexual development and might be useful for better practices of livestock management in seasonal breeders.

scholarly journals Ameliorative effects of Spinacia oleracea on sperm morphology, count, and motility by normalizing the obesity-induced oxidative stress in Sprague Dawley rats

2020 ◽  
Vol 14 (03) ◽  
pp. 124-127
Author(s):  
Somia Iqbal ◽  
Noman Sadiq ◽  
Saad Siddiqui ◽  
Hira Iqbal
Keyword(s):  
Sperm Concentration ◽  
Treated Group ◽  
Sperm Parameters ◽  
Control Group ◽  
Sprague Dawley ◽  
Normal Morphology ◽  
Sprague Dawley Rats ◽  
Group A ◽  
Group B ◽  
Experimental Group

Background: Obesity is a prevailing metabolic disorder that affects the functioning of the male reproductive system. Excessive adipose tissue enhances reactive oxygen species generation and is linked with male infertility. Spinach has demonstrated antioxidant effects. The present study was conducted to determine the antioxidant effects of spinach on sperm parameters in obese Sprague Dawley rats. Subjects and methods: This randomized control study was conducted at the animal house of the National Institute of Health Islamabad, Islamic International Medical College, Cosmesurge International Hospital, Rawalpindi, and Apollo lab, Islamabad, Pakistan from April 2016 to March 2017. Forty male Sprague Dawley rats having an age of 8 weeks and weight 160-200g were tagged from number 1 to 40. Every third rat was randomly allocated to control Group A (n=13) and remaining into the Experimental group (n=27). Rats of control Group A was given a standard diet while a high-fat diet was given to Experimental group rats to induce obesity for the duration of six weeks. Weight (g) was measured weekly and obesity was confirmed when rats attain more than 20% weight when compared with that of rats of control Group A. Then, after obesity induction, the experimental group was alienated into the obesity control group (Group B) and spinach treated group (Group C). For sample, rats of Group A and Group B were sacrificed, and the cauda epididymis of each rat was placed in a Petri dish containing normal saline and cut into pieces to allow the release of sperm and then sperm parameters (sperms concentration, motility, and morphology) were recorded under the microscope. Then, spinach (5% hot water extract) along with the persistence of fat diet was administered to Group C for 4 weeks and finally, sperm parameters were measured in this group. Results: Sperm concentration/ml, motility (%), and normal morphology (%) of Group B rats were significantly decreased as compared to Group A rats. However, sperm concentration/ml, motility (%), and normal morphology (%) of Group C (spinach treated group) rats was significantly increased (p<0.001) as compared to Group B (obesity control group) rats after administering spinach. Conclusion: The addition of Spinach in a normal diet regimen restores normal sperm morphology, improves sperm motility and concentration.

scholarly journals The effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats

2017 ◽  
Vol 1 (1) ◽  
pp. 28
Author(s):  
Eviana Budiartanti Sutanto ◽  
Taufiq R Nasihun ◽  
Israhnanto Isradji ◽  
Luciana Budiati Sutanto
Keyword(s):  
Vitamin C ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Control Group ◽  
Control Group Design ◽  
Normal Sperm ◽  
Post Test ◽  
Male Wistar Rats ◽  
Post Hoc ◽  
Per Oral

Introduction: Cigarette smoke causes oxidative stress which results in reduced sperm concentration, motility and morphology, also increased levels of 8-OHdG as a marker of DNA damage. Vitamin C and E have potential role in repairing spermatozoa damages. The aim of this study was to determine the effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats.Methods: This study used a post test only control group design among 18 male Wistar rats subject, aged 8 week, 150-200 grams body weight (BW). The subject was randomly divided into 3 groups, K1: control, K2: cigarettes smoke exposed, K3: cigarettes smoke exposed and given a combination of 0.045 mg/gBW vitamin C and 0.036 IU/gBW vitamin E per oral. Analysis was done on day 21 using one-way ANOVA and post-hoc LSD for sperm concentration, motility and morphology; using Kruskal-Wallis and Mann-Whitney tests for cement 8- OHdG levels.Results: The lowest sperm concentration was found in   K2 (K2  32.59  million/mL,  K1 47.91 million/mL, K 339.43 million/mL); the lowest normal sperm motility was found in K2 (K 238.97%, K 164.57%, K3 51.43%); the lowest normal sperm morphology was found in K2 (K2 27.56%, K 138.36%, K 331.18%); and the highest cement 8- OHdG level was found in K2 (K2 20.18ng/mL, K1 3.43ng/mL, K3 5.28ng/mL).Conclusion: Combination of vitamin C and E can improve sperm concentration, motility and morphology and decrease cement 8-OHdG levels of smoke exposed rats.

scholarly journals Increased F2-Isoprostane Levels in Semen and Immunolocalization of the 8-Iso Prostaglandin F2α in Spermatozoa from Infertile Patients with Varicocele

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Giulia Collodel ◽  
Elena Moretti ◽  
Mariangela Longini ◽  
Nicola Antonio Pascarelli ◽  
Cinzia Signorini
Keyword(s):  
Fatty Acid ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Prostaglandin F2α ◽  
Fertility Index ◽  
Idiopathic Infertility ◽  
Prostaglandin F ◽  
Sperm Midpiece ◽  
Infertile Patients

Polyunsaturated fatty acid damages lead to alterations in sperm function. This study aimed to investigate the involvement of F2-isoprostanes (F2-IsoPs), oxidized lipid products from arachidonic acid, in sperm quality impairment. For this purpose, F2-IsoP levels in semen and F2-IsoP localization in spermatozoa were explored in infertile subjects affected by idiopathic infertility or varicocele, as well as in fertile men. As compared to fertile men, in the idiopathic infertility and varicocele groups, sperm concentration, motility, morphology, viability, and fertility index were significantly lower and the mean scores concerning sperm apoptosis, necrosis, and immaturity were significantly higher. The idiopathic infertile group showed a reduction in sperm motility and fertility index, as well as an increase of apoptosis and necrosis percentages, in comparison to the varicocele group. The varicocele group showed the highest levels of F2-IsoPs, a significant increase of sperm immaturity, and a significant correlation between F2-IsoP levels and sperm immaturity. 8-Iso Prostaglandin F2α, biomarker of in vivo F2-IsoP, was clearly localized in sperm midpiece and cytoplasmic residues. Data show that F2-IsoP formation is relevant in semen and sperm from infertile patients with varicocele and high percentage of immaturity, suggesting that a correct fatty acid integrity is needed for sperm maturation.

The effects of chronic administration of ghrelin on rat sperm quality and membrane integrity

2009 ◽  
Vol 59 (2) ◽  
pp. 159-168 ◽  
Author(s):  
Arash Kheradmand ◽  
Majid Taati ◽  
Homayoon Babaei
Keyword(s):  
Plasma Membrane ◽  
Treatment Group ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Chronic Administration ◽  
Control Group ◽  
Plasma Membrane Integrity ◽  
Significant Difference

AbstractAlthough ghrelin acts as a modulator of feeding behavior and energy metabolism in the central nervous system, recent studies have implicated the peripheral actions of ghrelin in reproductive tissues. Here, we investigated the effects of chronic administration of ghrelin on the motility, plasma membrane integrity and concentration of rat spermatozoa. 45-d male Wistar rats were scheduled for the study and were divided into control and treatment groups. In the treatment group, 1 nmol of ghrelin was administered as sc injection for 10 consecutive days or vehicle (physiological saline) to the control rats. Sperm collection was achieved by killing of the rats on days 15, 25 and 50 after first injection. Total sperm motility and forward progressive movement did not exhibit significant difference during the experiment, although, there was a tendency for greater motion rate on d 15 and 25 in the treated rats compared to the control group. Plasma membrane integrity (HOS-reacted spermatozoa) was significantly higher in the treated animals, especially on day 15 as well as day 25, because of possible antioxidant properties of ghrelin. This value was statistically higher on day 15 than that of day 25 (P <0.05). Likewise, there was a significant correlation between the FPM (P <0.0001, r = 0.79) and TSM (P <0.01, r = 0.52) with the HOS test percentage in the treatment group. It was not observed statistically difference in the sperm concentration between groups during all of the experimental days. In conclusion, chronic administration of ghrelin (similar to induced by energy deficiency such as fasting) increased the integrity of sperm membrane, however, the sperm motility and concentration did not display any alterations.

scholarly journals The effect of a supplement of chromium (picolinate) on the level of blood glucose, insulin activity and changes in laboratory evaluation of the ejaculate of breeding boars

2012 ◽  
Vol 60 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Pavel Horký ◽  
Petra Jančíková ◽  
Ladislav Zeman
Keyword(s):  
Blood Glucose ◽  
Sperm Concentration ◽  
Laboratory Evaluation ◽  
Chromium Picolinate ◽  
Control Group ◽  
Organic Form ◽  
Laboratory Values ◽  
Insulin Activity ◽  
Experimental Group ◽  
Significant Data

The experiment was based on feeding the organic form of chromium (picolinate) and the assessment of its effect on the level of blood glucose, insulin activity and changes in the laboratory values of the ejaculate (sperm motility, ejaculate volume, sperm concentration and per cent of pathological sperm) in breeding boars. The experiment involved 40 boars divided into two equal groups. Boars of the experimental group (n = 21) received 181.81 μg of chromium per kg of feed ration (FR) administered perorally, in the control group (n = 19) chromium intake was not increased. The chromium supplement significantly (P < 0.05) increased cell absorption of blood glucose in the experimental group of boars as against the control group. There was no difference in insulin activity between the two groups. Changes in laboratory values of the ejaculate were evaluated and no significant differences were discovered in any of the parameters. During the experiment the sperm concentration and motility were absolutely the same in boars of both groups; it can therefore be concluded that increasing the level of chromium in the feed ration of boars of the experimental group had no direct effect on these parameters. It was the same in the case of the ejaculate volume which gradually decreased between periods 1 and 4, in boars receiving chromium by 12.8 % and in the control group by 8.1 %. The beneficial effect of chromium was seen in the reduced count of pathological sperm in boars in periods 3 and 4 of tests as against boars of the control group. In spite of the considerable 26.3 % difference between the groups this decrease was not statistically significant. Data evaluation revealed a significant correlation (P < 0.01) between the number of samplings per boar and sperm concentration. A correlation (P < 0.05) was also detected between the ejaculate volume and sperm concentration

Letrozole Protects Against Cadmium-induced Inhibition of Spermatogenesis via LHCGR and Hsd3b6 to Activate Testosterone Synthesis in Mice

2021 ◽  
Author(s):  
Yao Yao ◽  
Yangyang Wan ◽  
Xiaoyun Shi ◽  
Lan Guo ◽  
Hui Jiang ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Sperm Count ◽  
Hormone Secretion ◽  
Reproductive Toxicity ◽  
Plasma Testosterone ◽  
Control Group ◽  
Rna Seq ◽  
Testosterone Levels ◽  
Letrozole Treatment ◽  
Testosterone Synthesis

Abstract The heavy metal cadmium is believed to be one of the environmental endocrine disruptors of spermatogenesis. Cadmium-induced inhibition of spermatogenesis is associated with hormone secretion disorder. Letrozole is an aromatase inhibitor that can raise peripheral androgen levels and stimulate spermatogenesis. However, the potential protective effects of letrozole against cadmium-induced reproductive toxicity remain to be elucidated. In this study, male mice were administered CdCl2 (4 mg/kg BW) orally by gavage alone or in combination with letrozole (0.25 mg/kg BW) for 30 days. Cd exposure caused a significant decrease in body weight, sperm count, motility, vitality and plasma testosterone levels. Histopathological changes revealed extensive vacuolization and decreased spermatozoa in the lumen. However, in the Cd+letrozole group, letrozole treatment compensated for deficits in sperm parameters (count, motility, and vitality) induced by Cd. Letrozole treatment significantly increased serum testosterone levels, which were reduced by Cd. Histopathological studies revealed a systematic array of all germ cells, a preserved basement membrane and relatively less vacuolization. For mechanistic exploration, RNA-seq was used to profile alterations in gene expression in response to letrozole. Compared with that in the Cd-treated group, RNA-Seq analysis showed that 214 genes were differentially expressed in the presence of letrozole. Gene ontology (GO) enrichment analysis and KEGG signaling pathway analysis showed that steroid biosynthetic processes were the processes most affected by letrozole treatment. Furthermore, we found that the expression of the testosterone synthesis-related genes LHCGR (luteinizing hormone/choriogonadotropin receptor) and Hsd3b6 (3 beta- and steroid delta-isomerase 6) was significantly downregulated in Cd‐induced testes, but in letrozole-treated testes, these genes maintained similar expression levels as the control group. However, the transcription levels of inflammatory cytokines, such as IL-1β and IL-6, and oxidative stress-related genes (Nrf2, Nqo1, and Ho-1) showed no changes. The present study suggests that the protective potential of letrozole against Cd-induced reproductive toxicity might be due to upregulation of LHCGR and Hsd3b6, which could beneficially increase testosterone synthesis to achieve optimum protection in sperm quality and spermatogenesis.

scholarly journals Effect of supplementation with inorganic and organic selenium on sperm quality and quantity in north-east Bulgarian merino rams

2018 ◽  
Vol 34 (1) ◽  
pp. 69-81 ◽  
Author(s):  
Rossen Stefanov ◽  
Mihail Chervenkov ◽  
Georgi Anev ◽  
Nevena Maksimovic ◽  
Madlena Andreeva ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Antioxidant Defenses ◽  
Semen Parameters ◽  
Control Group ◽  
Organic Selenium ◽  
North East ◽  
Selenium Treatment ◽  
Experimental Group ◽  
Inorganic And Organic

Selenium is a trace element, which stimulates antioxidant defenses and improves reproductive functions in human and animals, under the form of selenoproteins. The objective of the study was to evaluate the effect of selenium, supplemeted as inorganic or organic form in the diet of stud rams, on some of their semen parameters. The experiment was performed with 15 clinically healthy rams from North East Bulgarian merino breed. The animals were divided in three groups (5 per group). The rams from first experimental group (G1) received a diet with supplementation of 4,0mg sodium selenite (NapH of the ejaculates and motility, concentration and in vitro survivability of the spermatozoa at 39?? for 360 min. 2SeO3) per animal per day, while the animals of the second experimental group (G2) obtained diet with 1.83g L-selenomethionine (Sel-Plex, Alltech, USA) per animal per day. Eventually, each animal from the G1 and G2 received 1.83g selenium per day. The control group (GC) received a diet without supplementation of selenium. The principal composition of the diet in each group was the same. The ejaculates were obtained via artificial vagina. The evaluated parameters were volume and It was found that the supplementation of ram studs diet either with inorganic and organic selenium led to increase in the volume of the ejaculates, motility and survivability of the spermatozoa. The pH of the freshly obtained semen was not affected by selenium treatment.

scholarly journals Effect of selenium and vitamin E supplementation on reproductive performance of young boars

2005 ◽  
Vol 48 (1) ◽  
pp. 68-75 ◽  
Author(s):  
A. Kołodziej ◽  
E. Jacyno
Keyword(s):  
Vitamin E ◽  
Seminal Plasma ◽  
Reproductive Performance ◽  
Morphological Changes ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Selenium Concentration ◽  
Control Group ◽  
Young Boars ◽  
Experimental Group

Abstract. The studies were carried out on 40 young boars of the synthetic line 990. On their 70 days of age, the boars were divided into two groups; the control group received 0.2 mg Se and 30 mg vitamin E while the experimental group received 0.5 mg Se + 60 mg vitamin E per 1 kg of feed mixtures. The feeding test was carried on from 70 days until 180 days of age. During the experiment the boars were subjected to live evaluations, i.e. testes volume, libido level, semen characteristics, as well as selenium concentration and glutathione peroxidase (GSH-Px) activity in blood serum and seminal plasma. The boars of the experimental group, compared with the control, showed significantly (P ≤ 0.05) higher sperm concentration and total sperm count, significantly lower (P ≤ 0.05) percentage of spermatozoa with major or minor morphological changes, elevated (P ≤ 0.05) percentage of spermatozoa with normal acrosome, and significantly higher (P ≤ 0.01) ORT values. GSH-Px activity was higher (P ≤ 0.05) in seminal plasma of the control group boars.

scholarly journals Marsilea crenata ethanol extract prevents monosodium glutamate adverse effects on the serum levels of reproductive hormones, sperm quality, and testis histology in male rats

2021 ◽  
pp. 1529-1536
Author(s):  
Sri Rahayu ◽  
Riska Annisa ◽  
Ivakhul Anzila ◽  
Yuyun Ika Christina ◽  
Aries Soewondo ◽  
...  
Keyword(s):  
Leydig Cells ◽  
Sperm Quality ◽  
Monosodium Glutamate ◽  
Ethanol Extract ◽  
Cell Number ◽  
Reproductive Hormones ◽  
Male Rats ◽  
Control Group ◽  
Testosterone Levels ◽  
Testis Histology

Background and Aim: Marsilea crenata is an aquatic plant that contains high antioxidants level and could prevent cell damages caused by free radicals. The present study aimed to investigate the effect of M. crenata ethanol extract on luteinizing hormone (LH), testosterone levels, sperm quality, and testis histology of adult male rats induced by monosodium glutamate (MSG). Materials and Methods: This study randomly divided 48 male rats into eight groups (n=6): control group; MSG group (4 mg/g body weight [b.w.] for 30 days); MS1, MS2, and MS3 groups (4 mg/g b.w. MSG and M. crenata ethanol extract at dose 0.216, 0.432, and 0.648 mg/g b.w., respectively, for 30 days); and S1, S2, and S3 groups (M. crenata ethanol extract at dose of 0.216, 0.432, and 0.648 mg/g b.w., respectively, for 30 days). The blood sample was collected on days 0 and 30 to determine the LH and testosterone levels. The animals were dissected on day 30, and the testes were isolated for morphometric, histology (spermatogenic cell number), and malondialdehyde (MDA) examination. Moreover, semen was collected to determine the sperm quality parameter. Results: The LH and testosterone levels significantly increased (p<0.05) after M. crenata administration at all doses. The higher dose of M. crenata ethanol extract demonstrated a high decrease in MDA level in MSG-treated rat testis; increase of spermatogonia, spermatocytes, spermatids, and Leydig cells number; and increase of seminiferous tubular diameter and germinal epithelium thickness. Conclusion: The ethanol extract of M. crenata can improve the levels of LH, testosterone, sperm quality, number of testis morphometric, spermatogenic, and Leydig cells in MSG-treated male rats.

scholarly journals Pengaruh Gangguan Tidur Terhadap Kadar Hormon Testosteron dan Jumlah Spermatozoa pada Tikus Jantan Wistar

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Leni Tri Wahyuni ◽  
Adnil Edwin Nurdin ◽  
Eliza Anas
Keyword(s):  
Sperm Quality ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Dependent Variables ◽  
Post Test ◽  
Independent Variable ◽  
The Mean ◽  
Experimental Group

Abstrak Tingginya angka infertilitas pada pria disebabkan antara lain oleh kualitas produksi spermatozoa dan gangguan hormonal. Tujuan penelitian ini adalah untuk mengetahui pengaruh gangguan tidur terhadap kadar hormon testosteron dan jumlah spermatozoa. Jenis penelitian adalah eksperimen laboratorium dengan desain post test only control groupdesign. Populasi tikus jantan Wistar berumur 2-3 bulan, dan berat badan 300 – 350 gr. Sampel sebanyak 24 ekor dibagi atas 4 kelompok yaitu: 1 kelompok kontrol dan 3 kelompok perlakuan. Variabel independen adalah gangguan tidur dan variabel dependen adalah kadar hormon testosteron dan jumlah spermatozoa. Analisa data mengunakan metode ANOVA dan dilanjutkan dengan uji statistik Multiple Comparisons jenis Bonferroni. Hasil penelitian menunjukkan ada perbedaan bermakna rerata kadar hormon testosteron kelompok kontrol dan perlakuan dengan nilaip=0,000. Gangguan tidur memberikan perbedaan bermakna terhadap kadar hormon testosteron. Terdapat perbedaan bermakna rerata jumlah spermatozoa kelompok kontrol dengan perlakuan dengan nilai p=0.000. Gangguan tidur juga memberikan perbedaan bermakna terhadap jumlah spermatozoa. Kesimpulan penelitian ini ialah terdapat pengaruh yang bermakna antara gangguan tidur terhadap kadar hormon testosteron dan jumlah spermatozoa pada tikus jantan wistar. Kata kunci: gangguan tidur, hormon testosteron, jumlah spermatozoaAbstract The increasing of infertility is caused by the quality of sperm production and hormonal disturbance. The objective of this study was to find out the effect of sleeping disturbance to the quality of testosterone hormone and the number of sperm. This was a laboratory experimental research with post-test only control group design. The populations were 2-3 months, 300-350 grams of weight, male rats. The sample was 24 rats which consisted of 4 groups: one control group and three experimental groups. Sleeping disturbance was an independent variable, whilethe quality of sperm testosterone hormone and the number of sperm were dependent variables. The data of the research were analyzed statistically by  ANOVA method and Bonferroni multiple comparison. There was a significant different of the mean of testosterone hormone quality between control group and experimental group with p=0,000. Sleeping disturbance gave significant different to the quality of testosterone hormone. There was a significant different on the number of sperm quality between control group and experimental group with p=0,000. Sleeping disturbancegave significant different to the number of sperm quality. The conclusion from this study is that there is effect of sleeping disturbance to testosterone hormone quality and the number of sperm on the wistar male rats. Keywords: sleeping disturbance, testosterone hormone, the number of sperm

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