scholarly journals The Effects Of Heavy Metals On The Morphological And Biochemical Composition Of The Blood Of Young Cattle Taking Into Account Of Breed Features

2020 ◽  
Author(s):  
Rustem B. Temiraev ◽  
Yusup A. Yuldashbaev ◽  
Susanna K. Cherchesova ◽  
Sofia F. Lamarton ◽  
Valentina S. Gappoeva ◽  
...  

AbstractHeavy metals can selectively accumulate in certain organs and remain in them for a long time. As a result, the accumulation of metal in one or another organ may cause strongest intoxication of the animal. The purpose of the research is to study the morphological and biochemical composition of the blood of young cattle of different breeds of the dairy and dairy-and-beef direction of productivity fed in the technogenic zone in the RNO-Alania diets with a high content of heavy metals. According to the principle of analogues, taking into account the breed, origin, age, sex and body weight, 4 groups of 10 animals each of different breed were formed: Group I - black and white, Group II - Danish Red, Group III - Swiss, and Group IV - Simmental. The experimental material was statistically processed using the Microsoft Excel software for mathematical analysis. With a tendency of unreliable differences in morphological indices of blood, in diminishing numbers of erythrocytes and hemoglobin, the test breeds should be arranged in the following order: Simmental → Swiss → Red Danish → Black and White. At the same time, as compared to the animals of the black-and-white breed, the young cattle of the Simmental breed showed the best level of intermediate metabolism, which resulted in an increase in total protein and sugar in the blood, which indicates an improvement in their protein and energy metabolism; an increase in the content of albumin in the liquid internal medium and γ-globulins;an increase inconcentration oftotal lipids in serum, respectively, while reducing the level of cholesterol, which indicates an improvement fat metabolism in animals; a significant (P <0.05) decrease in blood saturation with cadmium, lead and zinc, respectively, by 26.8, 48.8 and 30.6%. The highest content of these elements was noted in the wool, then in decreasing order: in bone tissue → liver → muscle tissue → lungs → kidneys. Moreover, between the level of heavy metals in muscles, liver and lungs, on the one hand, and in the wool, bone tissue and kidneys, on the other hand, there was an inversely proportional relationship.

Author(s):  
S.A. OVODKOV ◽  
Ah.S. DELYAN

Изучено влияние способа содержания и сезона отела на лактационную деятельность голштинизированных коров-первотелок черно-пестрой породы. Исследования проведены в условиях сельскохозяйственного предприятия Тверской области на 4 группах животных: I группа — привязное содержание, летние отелы; II — привязное содержание, зимние отелы; III — беспривязное содержание, летние отелы; IV — беспривязное содержание, зимние отелы. Максимальные удои у коров I, III и IV групп были получены на 3-м мес лактации, II — на 2-м. Удой в I, II, III и IV группах составил, соответственно, 7714, 8717, 7682 и 7930 кг молока за 305 дней лактации. Удой коров за первые 3 мес в % к удою за 305 дней лактаций: I группа — 33,7%, II — 35,8, III — 34,4, IV — 35,9%. Максимальный суточный удой в I группе равнялся 30,8 кг, во II — 36,5, в III —29,7 и в IV — 35,3. Среднесуточный удой за 305 дней лактации в I группе составил 21,2 кг, во II — 23,6, в III группе — 19,4 и в IV группе — 21,7. Коэффициент постоянства лактации в I II III и IV группах равнялся 93,2, 94,2, 89,2 и 98%, соответственно. Независимо от способа содержания более высокие показатели молочной продуктивности и устойчивости лактационной деятельности имели коровы, отелившиеся в зимний период.The influence of the method of keeping and calving season on the lactation activity of Holstein cows-heifers of black-and-white breed is studied. Studies were conducted in the agricultural enterprise of the Tver region on 4 groups of animals: I group — tethered content, summer calving; 2 — tethered content, winter calving; III — loose content, summer calving; 4 — loose content, winter calving. Maximum milk yield in cows I and III and IV groups were obtained on the 3rd month of lactation, the second — at the 2nd. Milk Yield in I, II and III and in IV groups made, respectively, 7714, 8717, 7682 and 7930 kg of milk for 305 days of lactation. Milk yield of cows for the first 3% to milk yield for 305 days of lactation: group I-33,7%, II — 35,8, III-34,4, IV-35,9%. The maximum daily yield in group I was 30.8 kg, in group II — 36.5, in group III — 29.7 and in group IV — 35.3. The average daily yield for 305 days of lactation in group I was 21.2 kg, in group II — 23.6, in group III — 19.4 and in group IV — 21.7. The coefficient of persistence of lactation in I, II, III and IV groups was equal to 93.2 per cent, 94,2, to 89.2 and 98%, respectively. Regardless of the method of maintenance, cows calving in winter had higher indicators of milk productivity and stability of lactation activity.


The transplantation of tissues raises many yet unsolved problems of fundamental interest, and is of potentially great clinical importance, especially in endocrinology. The present investigation is concerned with three main problems: the immunological reactions associated with the growth of homografts in the anterior chamber of the eye and subcutaneously; the role of the reticulo-endothelial system in the resistance to homografts; and the applicability of Halsted’s principle to auto- and homografts of endocrine tissue, and the mechanism involved. Four groups of experiments are described. In group I, a preliminary study of auto- and homografts of thyroid in the guinea-pig, it is shown that, if a total thyroid deficiency is produced in the host, autografts are uniformly successful both in the anterior chamber and subcutaneously, whereas homografts are usually successful in the anterior chamber but rarely so when made subcutaneously. Halsted’s principle holds good for anterior chamber grafts whether auto- or homografts, i.e. the proportion of successful grafts increases pari passu with the degree of thyroid deficiency produced in the host, but appears to be less applicable to subcutaneous autografts. Some light is thrown on the mechanism of Halsted’s principle by the observation that successful anterior chamber grafts can be obtained in non-thyroidectomized hosts if injections of pituitary thyrotrophic hormone are given. The experiments of group II are concerned with the immunological phenomena relating to thyroid homografts. It is shown that a subcutaneous homograft of thyroid in the guinea-pig is demonstrably antigenic and the same is true of a homograft in the anterior chamber provided this is undergoing destruction. The state of immunity induced is general and extends to the anterior chamber. The existence of a state of immunity induced by a homograft is shown by the diminished chance of a subsequent homograft ‘taking’, but ‘immunization’ (by means of a subcutaneous graft) of an animal which already has a homograft established in the anterior chamber does not appear to modify the behaviour of the latter. In group III it is shown that a homograft transferred after some months from the anterior chamber to a subcutaneous site is more likely to survive than a primary subcutaneous homograft. It thus appears that when a homograft is established in the anterior chamber for a sufficiently long time a process of adaptation occurs as between graft and host. The experiments of group IV relate to the behaviour of auto- and homografts of spleen in the anterior chamber, and of simultaneous thyroid and splenic grafts. It is shown that the chances of success of a thyroid homograft in the anterior chamber are greatly reduced if the latter is provided with reticulo-endothelial tissue in the shape of a simultaneous autograft of spleen, but that a simultaneous homograft of spleen from the same donor has little or no deleterious effect. The mechanism of this phenomenon has not been fully elucidated but would appear to be either cellular, humoral or a combination of the two.


Author(s):  
T. A. Krasnoshchekova ◽  
O.N. Tyutyavkina

The purpose of the research was to make a comparative study of the use of enzymatic probiotics in the feeding of young cattle Black-and-White breed and to determine their effect on growth, development and metabolic processes. Under the conditions of the Amur region the use of enzymatic probiotics in the feeding of young cattle has been justified in two scientific and economic experiments. In physiological experiments the digestibility of nutrients in rations has been determined; the balance of nitrogen, calcium and phosphorus has been studied. As a result of the first scientific and economic experiment, a comparative study of the feeding of enzymatic probiotics Cellobacterin and Vitacell to calves has been carried out. It has been found that the inclusion of the enzymatic probiotic Vitacell in the rations of calves had a better effect on growth, digestibility and balance of nutrients as well as improved hematological parameters, bringing them to the physiological norms. In the second experiment the effect of the enzymatic probiotics Vitacell and Promilk on the dynamics of the live weight of young cattle, their growth, development and metabolic processes has been studied. It has been found that the enzymatic probiotic Vitacell had a better effect on growth, digestibility and nutrient balance compared to the probiotic Promilk. Thus, physiological, biochemical, zootechnical and economic research methods proved the feasibility of using probiotics in the rations of calves of Black-and-White breed, but the best results were obtained when using the probiotic Vitacell. The data of the production inspection confirmed the results obtained in scientific and economic experiments.


2020 ◽  
Vol 59 ◽  
pp. 17-25
Author(s):  
S. F. Antonenko

The scientific experiment was carried out in two stages at State enterprise pilot farm “Hontarivka”, Vovchansk district, Kharkiv region. First stage. In a retrospective analysis of the rearing calf intensity from 3 to 6 months revealed that the highest live weight at 6-month age had animals of group III, .which is probably 47.0 kg or 34.6% and 23.0 kg or 14.4% (P < 0.001 in both comparison cases) outperformed peers I and II groups. A similar trend was observed in the analysis of live weight of heifers at 9 and 12-month age. Live weight at insemination in the higher growth group also had better rates of 4.0 and 3.7%, respectively, in animals of groups I and II (P < 0.001–0.05). It should be noted that the required live weight for insemination at heifers at growth rates of up to 500 g was reached in only 20.3 months, however, in the other two groups, the insemination age decreased by 1.5 and 1.7 months. Firstcalf heifers from group III also had the highest milk expectancy of 170.0 and 123.0 kg, or 3.1 and 2.3%, respectively, then animals in groups I and II. In the age period from 6 to 9 months it was found that at 9-month age heifers of group III had a higher live weight against animals of I and II groups respectively by 39.0 and 16.0 kg or 18.8 and 7.0% (P < 0.001 in both comparison cases). Intergroup differences by this indicator at 12-month age hiefers decreased to 35.0 and 8.0 kg, respectively, or 13.0 and 2.7% (P < 0.001 in both cases of comparison), which provided an advantage for the live weight of group III over I and II groups during the first fruitful insemination. Whereas the age of first fruitfull insemination, on the contrary, was lower at heifers of group III at 49 and 34 days than at groups I and II heifers. The highest milk productivity was also found at firstcalf heifers of group III at 634 kg or 12.3% (P < 0.001) and at 137 kg or 2.5% against animal I and II groups. The heifer live weight at the period from 9 to 12 months corresponded to the established differences at other age periods generally. And 12-month age calves of group III also significantly exceeded the analogues of group I by 38.0 kg or 14.0% (P < 0.001) and peers of group II – by 23.0 kg or 8.0% (P < 0.001). The live weight at fruitfull insemination of group III heifers was the largest and the difference compared with animals of group I was 21.0 kg or 5.3% (P < 0.01) and group II – 19.0 kg or 4.8% (P < 0.001). Group III animals also had the highest milk productivity, which is likely to be higher than group I peers by 751 kg or 14.5% (P < 0.001) and group II animals by 552 kg or 10.3% (P < 0.001). Second stage. It was found that during the experiment, live weight of free-stall keeping heifers were outnumbered by peers. In particular, the live weight of group I animals at the first month of the experiment was less by 8.0 kg or 2.9%, the second by 15.0 kg or 4.7% (P < 0.05) and the third by 19.0 kg or 5.4% against peers of group II. A similar pattern was observed in the estimation of intensive growth of these animals. However, at the end of the experiment, the heifers of the experimental group were 7.0 cm or 5.9% (P < 0.001) higher in the withers, 7.0 cm or 5.6% (P < 0.001) in the back, and the sacrum – 6.0 cm or 4.7% (P < 0.001). They showed deeper breasts by 3.0 cm or 4.9% (P < 0.01), larger breast widths – by 2.0 cm or 5.4% (P < 0.01), width in macaques – by 2.0 cm or 4.9% (P < 0.01), oblique torso length – 8.0 cm or 5.8% (P < 0.001), chest circumference 9.0 cm or 5.6% (P < 0.001) and girth – 1.0 cm or 6.1% (P < 0.01). It should be noted that from the second month of the research the animal number the free-stall keeping animal number, which ready for insemination, was more at 2.3 times or 5 goals than stall keeping heifers, and their live weight was more at 8 kg or 3.0%. With age, the differences between the groups gradually increased and by the third month reached 8 goals or 2.6 times and 19.0 kg or 5.7%.


2019 ◽  
Vol 30 (2) ◽  
pp. 113-117
Author(s):  
S. V. Yermolenko ◽  
V. Ya. Gasso ◽  
A. M. Hagut ◽  
I. A. Hasso ◽  
V. A. Spirina

We studied the peculiarities of bioaccumulation and distribution of heavy metals (cadmium, lead, zinc and copper) in the bone tissue, liver and kidneys of the dice snake Natrix tessellata (Laurenti, 1768) inhabited ecosystems under different anthropogenic loads in 2015-2016. Mature individuals were caught in anthropogenically transformed ecosystems of the sanitary-protective zone of the Dniprovska Thermal Power Plant (SPZ DTPP) (48.402°N, 35.111°E, n = 7), coastal ecosystems of the National Nature Park «Velykyi Luh» (47.443°N, 35.149°E, n = 8) and in the Maiorka gully ecosystems (48.260°N, 35.170°E, n = 7). The gross content of metals in the organs of N. tessellata was determined after their dry ashing in a muffle furnace. Determination of the trace elements was performed by atomic absorption spectrophotometry. Among the studied trace elements, zinc has the highest concentrations in the liver, kidneys and bones of N. tessellata. The kidneys and liver are characterized by such amount of concentrations: Zn> Cu> Pb> Cd, but bone tissue has the following ratio Zn> Pb> Cu> Cd. The highest concentration of cadmium is found in the kidneys, and the lowest one – in the bones of snakes. There are the highest contents of lead and copper in the snakes’ liver, but the lowest in the kidneys and bones. The lead bioaccumulations in organs of snakes from different ecosystems are dissimilar. Zinc content in bones is higher than in kidneys and liver, but the lowest concentration is found in the kidneys. It has been found that the highest contents of toxic metals (Cd, Pb) were in the kidneys and liver of the snakes dwelled in the SPZ DTPP ecosystems in comparison with both studied natural ecosystems. In addition, snakes caught in anthropogenically transformed ecosystems of SPZ DTPP are characterised by increased zinc content in all studied organs and enlarged copper concentration in the kidneys. Considering the obtained results on the metals bioaccumulation, the dice snake can be considered as a potential bio-indicator of environmental pollution by heavy metals.


2020 ◽  
Vol 104 (5) ◽  
pp. 36-43
Author(s):  
P. Leonenko ◽  
◽  
Yu. Kokoieva ◽  

Summary. Inflammation and pain can lead not only to a deterioration in the patient’s condition, but also to such local consequences as: bone resorption, loss of soft tissue volume, an increase in the wound healing time and patient rehabilitation in general. Inflammation-induced bone resorption in the area of implantation with direct prosthetics, caused by the activity of cytokines and prostaglandins, negatively affects the entire result of treatment of dentition defects in general. This is because the quality and quantity of bone tissue is one of the key points in the success of prosthetics on dental implants, therefore, pharmacological support of dental implantation and direct prosthetics is an important component of treatment. Purpose: to investigate the effect of inflammation and pain on peri-implant bone tissue at the stages of dental implantation and direct prosthetics and scientifically substantiate pharmacological support in order to prevent inflammatory bone resorption. Materials and methods. A clinical prospective study of 57 patients was carried out at the stage of dental implantation and direct prosthetics with randomization according to the type of pharmacological accompaniment: 1) group I received anti-inflammatory therapy in the form of a balanced inhibitor of COX-1, COX-2 and 5-LOG – nimesulide and analgesic therapy – dexketaprofen trometamol; 2) group II received anti-inflammatory therapy – a selective COX-2 inhibitor – meloxicam and analgesic therapy – ibuprofen; 3) group III did not receive anti-inflammatory and analgesic therapy due to contraindications to the use of non-steroidal anti-inflammatory drugs. Patients of groups I, II, III underwent: clinical, radiological and functional research methods by monitoring the state in dynamics. Results. According to the data obtained, the indices of pain intensity in group I were significantly lower (p < 0.05) as of 1 and 2-d days, compared with groups II and III. The stabilization of inflammatory processes in group I was recorded on the 2-d day. There was a significant decrease (p < 0.05) in the signs of the inflammatory process in patients of group I on the 3rd day (3.01±0.11 units), and on the 7-th day – their complete absence (1.12±0.23 units). In group II, significant regression of inflammation was noted on the 4th day (3.14±0.12 units), and on the 7-th day, minimal signs were observed (2.04±0.17 units). A decrease in signs of inflammation in group III occurred from the 5th day (3.31±0.28 units), and inflammatory phenomena were observed on the 7th day of the study (2.65±0.27 units). In group I, there was a significant stop in the loss of stability of the connection between the bone tissue and the dental implant on the 20-th day (65.08±1.03 points). As of the 25-th day, in patients of group I of the study, there was significantly higher (p < 0.05) indicators of the coefficient of stability of the implant (66.21±1.40 points) in relation to group II of patients (62.93±0.94 points), in who used selective COX-2 inhibitors, and group III (62.90±0.75 points), where NSAID’s were not used. The loss of marginal bone around the dental implant during the study period in group I was 0.5±0.23 mm CI, in group II – 1.1±0.34 mm, in group III – 1.3±0.28 mm. Side effects in group I of the study were recorded in 5.3 % of patients taking drugs nimesulide and dexketoprofen, and in 15.8 % of those in group II who took drugs meloxicam and ibuprofen. Conclusions. Complex pharmacological support of dental implantation and direct prosthetics on implants in the treatment of dentition defects, consisting of perioperative analgesia – dexketoprofen trometamol, as well as nimesulide for anti-inflammatory therapy, made it possible to influence the trauma-induced bone resorption in the implantation area by controlling inflammation. As a result, on the 20-th day in the patients of the group I of the study, a significant stop was noted in the loss of stability of the connection of the bone tissue and the dental implant (65.08±1.03 units), and on the 25-th day of the study in the group I it was found significantly higher (p < 0.05) indicators of the coefficient of stability of the implant and less loss of height of marginal bone tissue in relation to groups II and III of patients. This pharmacological complex made it possible to achieve stabilization of pathological processes in soft tissues – stopping the formation of edema on the 2-d day, a significant decrease (p < 0.05) of signs of the inflammatory process on the 3-d day (3.01±0.11 points) and to implement effective pain prevention at the stages of dental implantation and direct prosthetics.


2019 ◽  
pp. 300-307
Author(s):  
Laipanov ◽  
Begiev ◽  
Bittirov ◽  
Begieva

The article gives an assessment of the effect of the new complex anthelmintic preparation "Prazinal F" on milk productivity and on the biochemical composition of milk of cows of black and white breeds with paramphistomatosis. Determination of the optimal dose after milking the cows of the black and white breed of the new preparation "Prazinal F" with paramphistomatosis showed that during the second, third, fourth and fifth months of lactation for milk production, the dose of 25 mg / kg of body weight has the most beneficial effect. During the second, third and subsequent months of lactation after prescribing "Prazinal F" in a dose of 25 mg / kg body weight, the milk productivity of the cows of the experimental groups and, especially, of the third group is increased. The cows of the 3rd group in terms of gross milk yield exceeded the cows of the control group infected with paramphistomatosis by 36,0%. In comparison with the control infected group, the difference for the third month of lactation, respectively, was 2.9-3.7-4.5 kg in favor of the experimental groups, 3.0-3.5-4.4 kg for the fourth month and the fifth month – 2,6-3,8-4,6 kg. The use of "Prazinal F" in the paramphistomatosis of cows of black and motley breed at a dose of 25 mg / kg of body weight, once allowed to increase the content of iodine, cobalt, magnesium and iron in milk by 31.7-45.6%. The appointment of the drug "Prazinal F" in the paramphistomatosis of cows of black and motley breed at a dose of 25 mg / kg of body weight, once allowed to increase the production of cow's milk, significantly enriching it with nutrient microelements.


2021 ◽  
Vol 1 (1) ◽  
pp. 18-23
Author(s):  
A.N. Bigarina ◽  
◽  
K.H. Shaikenova ◽  

This article presents the results of research on the growth and development of calves obtained from cows during early insemination. The object of the study was calves from birth to 6 months of age of a black-and-white breed of a dairy farm «Kamyshenka» LLP in the Astrakhan district of the Akmola region. Two experimental groups of calves were formed in a dairy farm. Group I included calves from cows inseminated at 14-15 months of age, and group II included calves from cows inseminated at 16-17 months of age. The following indicators were studied: live weight of calves at birth and at the age of 6 months, average daily, absolute and relative increases in live weight were calculated. At the same time, the exterior profile was studied by measurements from birth to 6 months of age, followed by the calculation of body indices. The live weight of calves at birth in the first experimental group averaged 30.9±0,32 kg, while in the second it was at the level of 29.8±0,44 kg, and at the age of 6 months it was 149.2±2,3 kg and 147.3±2,4± kg, respectively. According to the exterior profile data, there are also better indicators in the first experimental group than in the second, since the oblique length of the trunk was 104.43±0,7 and 102.33±0,9 cm, respectively. The difference in body type indices ranged from 0.58-1.41%, respectively.


2016 ◽  
Vol 1 (1) ◽  
pp. 27-29 ◽  
Author(s):  
Гизатова ◽  
Natalya Gizatova

The research object is improvement of the productive qualities of heifers of Kazakh white breed by introducing additional to the basic diet of different doses of feed additives BioDarin. Protein-vitamin-mineral supplement BioDarin in its composition contains nutrients, micro- and macroelements, which are the source of energy, stimulate digestive processes, metabolism, growth and improve immunity, positive effect on animal performance. Currently of great practical and scientific importance is the use of the additive BioDarin for the correction of diet feeding. Scientific and economic experiment was carried out on the basis of JSC AIC «Alga+» Tuimazinsk district of the Republic of Bashkortostan. For the experiment there were formed 4 groups of heifers of Kazakh white-headed breed: group I – control; group II heifers in addition to the basic diet received a feed additive in a dose of 0.5 kg per 100 kg of grain mixture, the animals of group III received 1 kg of the feed additive per 100 kg of grain mixture, peers IV groups received 1.5 kg of a grain mixture. It is established that heifers of the III experienced group receiving feed additive BioDarin in the dose of 1.0 kg per 100 kg of grain mixture, had the best body weight, and average day growth.


2018 ◽  
Vol 17 (2) ◽  
pp. 224-229 ◽  
Author(s):  
Ayobola Abolape Iyanda

Background: Reports are available indicating that leaded gasoline is still being made available in some parts of the world. Moreover it is also known that the crude from which petroleum products are obtained is rich in heavy metals. This means heavy metal toxicity from petrol contact is a possibility. The aim of this study is to assess selected heavy metal levels in teenagers who are currently and formerly employed as GSA.Materials and Methods: The study population consisted of teenagers divided into 3 groups. GROUP I was made up of 30 male subjects that are currently employed as GSA with less than 6 months work history; GROUP II composed of 34 male teenagers with work history in gas station not less than 20 months; GROUP III consisted of 22 male teenagers who were former attendants; the control group consisted of 35 participants, with no history of occupational exposure to fuel, or heavy metals. Information was obtained through questionnaire on demography, life-style and clinical symptoms of gasoline exposure. Serum heavy metal levels were estimated using atomic absorption spectrometry. Student’s t test and analysis of variance were employed to determine the level of significant difference. P < 0.05 was considered significant.Results and Discussion: The serum levels of lead, arsenic, nickel, cadmium, and aluminium of GSA in GROUPS I and II were significantly higher compared with control. On the other hand, there were significant increases in levels of lead and cadmium in GROUP III compared with control. Additionally, majority of test subjects in GROUPS I and II identified headache and fatigue as symptoms associated with workplace. Teenage GSA are prone to heavy metal toxicity and discontinuing contact with gas station environment did not resolve this occupational hazard.Conclusion: Gonadotoxic effect of some of these elements on these categories of subjects who are within reproductive stage is possible. Therefore appropriate measure should be taken to address it.Bangladesh Journal of Medical Science Vol.17(2) 2018 p.224-229


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