scholarly journals Native Corrinoids from Clostridium cochlearium Are Adeninylcobamides: Spectroscopic Analysis and Identification of Pseudovitamin B12 and Factor A

2000 ◽  
Vol 182 (17) ◽  
pp. 4773-4782 ◽  
Author(s):  
Bernd Hoffmann ◽  
Michael Oberhuber ◽  
Erhard Stupperich ◽  
Harald Bothe ◽  
Wolfgang Buckel ◽  
...  
Keyword(s):  
Spectroscopic Data ◽  
Mass Spectra ◽  
Spectroscopic Analysis ◽  
Obligate Anaerobe ◽  
Sample Analysis ◽  
The Third ◽  
Absorbance Spectroscopy ◽  
Pseudovitamin B12 ◽  
Main Components ◽  
Clostridium Cochlearium

ABSTRACT The corrinoids from the obligate anaerobe Clostridium cochlearium were extracted as a mixture of Coβ-cyano derivatives. From 50 g of frozen cells, approximately 2 mg (1.5 μmol) of B12 derivatives was obtained as a crystalline sample. Analysis of the corrinoid sample ofC. cochlearium by a combination of high-pressure liquid chromatography and UV-Vis absorbance spectroscopy revealed the presence of three cyano corrinoids in a ratio of about 3:1:1. The spectroscopic data acquired for the sample indicated the main components to be pseudovitamin B12(Coβ-cyano-7"-adeninylcobamide) (60%) and factor A (Coβ-cyano-7"-[2-methyl]adeninylcobamide) (20%). Authentic pseudovitamin B12 was prepared by guided biosynthesis from cobinamide and adenine. Both pseudovitamin B12 and its homologue, factor A, were subjected to complete spectroscopic analysis by UV-Vis, circular dichroism, mass spectrometry, and by one- and two-dimensional 1H,13C-, and 15N nuclear magnetic resonance (NMR) spectroscopy. The third component was indicated by the mass spectra to be an isomer of factor A and is likely (according to NMR) to be 7"-[N 6-methyl]-adeninylcobamide, a previously unknown corrinoid. C. cochlearium thus biosynthesizes as its native “complete” B12 cofactors the 7"-adeninylcobamides and two homologous corrinoids, in which the nucleotide base is a methylated adenine.

scholarly journals Two new α-Methoxy-γ-Pyrones From the Mangrove Sediment-Derived Streptomyces psammoticus SCSIO NS126

2021 ◽  
Vol 16 (9) ◽  
pp. 1934578X2110414
Author(s):  
Kunlong Li ◽  
Mengdie Zhou ◽  
Ziqi Su ◽  
Xiliang Yang ◽  
Xuefeng Zhou ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Spectroscopic Data ◽  
Spectroscopic Analysis ◽  
Mangrove Sediment ◽  
Natural Sources ◽  
Fermentation Conditions ◽  
Acetylcholinesterase Inhibitory Activity ◽  
Actinomycete Strain

Two new α-methoxy- γ-pyrone analogs, 2-methoxy-3-methyl-5,6-diethyl- γ-pyrone (2) and 2-methoxy-3,5-dimethyl-6-propyl- γ-pyrone (3), together with 2-methoxy-3,5-dimethyl-6-ethyl- γ-pyrone (1), firstly isolated from natural sources, were obtained from the EtOAc-solube extract of the mangrove sediment-derived actinomycete strain Streptomyces psammoticus SCSIO NS126, under the optimized fermentation conditions. Their structures were elucidated by detailed spectroscopic analysis and by comparison of their spectroscopic data with those reported in the literature. Those α-methoxy-γ-pyrones were evaluated for their acetylcholinesterase inhibitory activity; however, none of them exhibited obvious activity. Moreover, their biosynthetic relationship with piericidins was also discussed.

scholarly journals Durian Rind Micro Composter Model: A Case of Kampung Durian, Ngrogung, Ponorogo, Indonesia

2021 ◽  
Vol 226 ◽  
pp. 00021
Author(s):  
Haris Setyaningrum ◽  
Atika Rukminastiti Masrifah ◽  
Adib Susilo ◽  
Imam Haryadi
Keyword(s):  
Air Pollution ◽  
Organic Fertilizer ◽  
Dry Season ◽  
Organic Fertilizers ◽  
Direct Impact ◽  
The Third ◽  
Main Components ◽  
Surrounding Areas ◽  
Durian Rind

The case of waste from durian rind in Ngrogung, Ngebel, Ponorogo, Indonesia increased significantly per year. Agglomerations and decomposition of durian rind surrounding agro park caused air pollution and bad view in park areas. The decomposition of durian rind is very important to reduce those impacts. The micro composter model in Kampung Durian consisted of three main components. The first component was drying area with cemented ground. The drying area was set to accommodate 1 t fresh durian rind per day. The materials come from surrounding areas of agro park. The second was a machine house with one ton material capacity per day. The third was a composter house consisted of composter tube and packing area. The result of the micro composter house showed a cleaning agglomeration of durian rind from Ngebel lake and surrounding areas. In addition, the model produced liquid organic fertilizer and compost. A cycle of composting was 30 d in the dry season. Amount 1 t of durian rind decomposition resulted of 100 L liquid organic fertilizers and 50 kg compost. The result of composter had a direct impact to control waste problems in Ngebel and surrounding areas.

scholarly journals Progressive Boundary Refinement Network for Temporal Action Detection

2020 ◽  
Vol 34 (07) ◽  
pp. 11612-11619
Author(s):  
Qinying Liu ◽  
Zilei Wang
Keyword(s):  
State Of The Art ◽  
The State ◽  
Action Detection ◽  
Fine Grained ◽  
The Third ◽  
One Stage ◽  
The Family ◽  
Main Components ◽  
Temporal Action ◽  
Boundary Refinement

Temporal action detection is a challenging task due to vagueness of action boundaries. To tackle this issue, we propose an end-to-end progressive boundary refinement network (PBRNet) in this paper. PBRNet belongs to the family of one-stage detectors and is equipped with three cascaded detection modules for localizing action boundary more and more precisely. Specifically, PBRNet mainly consists of coarse pyramidal detection, refined pyramidal detection, and fine-grained detection. The first two modules build two feature pyramids to perform the anchor-based detection, and the third one explores the frame-level features to refine the boundaries of each action instance. In the fined-grained detection module, three frame-level classification branches are proposed to augment the frame-level features and update the confidence scores of action instances. Evidently, PBRNet integrates the anchor-based and frame-level methods. We experimentally evaluate the proposed PBRNet and comprehensively investigate the effect of the main components. The results show PBRNet achieves the state-of-the-art detection performances on two popular benchmarks: THUMOS'14 and ActivityNet, and meanwhile possesses a high inference speed.

scholarly journals Pubinernoid A and Apo-9′-fucoxanthinone, Secondary Metabolites from a Gorgonian Coral Pinnigorgia sp

2016 ◽  
Vol 11 (6) ◽  
pp. 1934578X1601100
Author(s):  
Hsin-Huan Chang ◽  
Yu-Chia Chang ◽  
Wu-Fu Chen ◽  
Tsong-Long Hwang ◽  
Lee-Shing Fang ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Spectroscopic Data ◽  
Spectroscopic Analysis ◽  
Inhibitory Effect ◽  
Human Neutrophils ◽  
Gorgonian Coral ◽  
First Report ◽  
Significant Inhibitory Effect ◽  
Animal Source

The structures of pubinernoid A (1) and apo-9′-fucoxanthinone (2), isolated from a gorgonian coral Pinnigorgia sp., were elucidated on the basis of spectroscopic analysis and by comparison of their spectroscopic data with those of known compounds. This is the first report of 1 and 2 from an animal source. Apo-9′-fucoxanthinone (2) displayed a significant inhibitory effect on the release of elastase by human neutrophils, with an IC50 value of 5.75 μM.

scholarly journals GC-MS Chemical Characterization of Main Components of Smilax Domingensis Wild. in Cuba

2018 ◽  
Vol 5 (4) ◽  
pp. 79 ◽  
Author(s):  
Pilar A. Soledispa ◽  
José González ◽  
Armando Cuéllar ◽  
Julio Pérez ◽  
Max Monan
Keyword(s):  
Mass Spectra ◽  
Chemical Constituents ◽  
Chemical Characterization ◽  
Ethanolic Extract ◽  
Chemical Compounds ◽  
Chemical Components ◽  
Analytical Technique ◽  
Main Components ◽  
First Time

A preliminary chemical characterization of main components of ethanolic extract with dried rhizomes of Smilax domingensis Wid. that grow in Cuba was done using a GCMS-QP2010 Ultra Shimadzu and the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. After sample derivatization 125 chemical compounds were registered by the equipment and from them, 35 different chemical components were characterized and reported for the first time from this part of the plant in our country. The results demonstrate the developed method could be employed as a rapid and versatile analytical technique for identification of chemical constituents and quality control of Smilax domingensis.

scholarly journals Raman spectroscopic analysis of high molecular weight proteins in solution – considerations for sample analysis and data pre-processing

The Analyst ◽  
2018 ◽  
Vol 143 (24) ◽  
pp. 5987-5998 ◽  
Author(s):  
Drishya Rajan Parachalil ◽  
Brenda Brankin ◽  
Jennifer McIntyre ◽  
Hugh J. Byrne
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Multivariate Regression ◽  
Spectroscopic Analysis ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Sample Analysis ◽  
Raman Spectroscopic ◽  
Regression Techniques ◽  
High Molecular Weight Proteins

This study explores the potential of Raman spectroscopy, coupled with multivariate regression techniques and ion exchange chromatography, to quantitatively monitor diagnostically relevant changes in high molecular weight proteins in liquid plasma.

scholarly journals Prenylated Flavones from Artocarpus Lanceifolius and their Cytotoxic Properties against P-388 Cells

2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Iqbal Musthapa ◽  
Jalifah Latip ◽  
Hiromitsu Takayama ◽  
Lia D. Juliawaty ◽  
Euis H. Hakim ◽  
...  
Keyword(s):  
Spectroscopic Data ◽  
Mass Spectra ◽  
Cytotoxic Effect ◽  
Methanol Extract ◽  
2D Nmr ◽  
Tree Bark ◽  
Prenylated Flavones ◽  
Murine Leukemia

New prenylated flavones, artoindonesianins Z-4 and Z-5, together with four known prenylated flavones, artonin E, 12-hydroxyartonin E, artobiloxanthone, and cycloartobiloxanthone, have been isolated from the methanol extract of the tree bark of Artocarpus lanceifolius. The structures of these compounds were determined on the basis of spectroscopic data, including UV, IR, 1D and 2D NMR, and mass spectra. The cytotoxic effect of the isolated compounds against murine leukemia P-388 cells is described.

Gas-Liquid Chromatographic Determination of Chlordene Epoxide in Catfish

1978 ◽  
Vol 61 (1) ◽  
pp. 01-04
Author(s):  
Gabriel D Castillo ◽  
Marshall T Jeffus ◽  
Charles T Kenner
Keyword(s):  
Mass Spectra ◽  
Chromatographic Determination ◽  
Gas Liquid Chromatography ◽  
Edible Portion ◽  
The Third ◽  
Chemical Residues ◽  
Fish Samples ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Chlordene epoxide was detected in the range of 0.04 to 0.27 ppm in the edible portion of 3 catfish samples during the analysis of 36 fish samples by electron capture gas-liquid chromatography (GLC) for pesticide and industrial chemical residues. Two of these catfish samples were from suppliers in Louisiana and the third was from a Texas supplier. Chlordene was also present; the amount of chlordene epoxide in the 3 samples averaged 46% of the amount of chlordene present. The epoxide was identified by comparison of the mass spectra and the CLC retention times of the samples with reference material prepared in this laboratory.

Cerium intermetallics CeTX – review III

2016 ◽  
Vol 71 (3) ◽  
pp. 165-191 ◽  
Author(s):  
Rainer Pöttgen ◽  
Oliver Janka ◽  
Bernard Chevalier
Keyword(s):  
High Pressure ◽  
Solid Solutions ◽  
Spectroscopic Data ◽  
Structure Property ◽  
The Third ◽  
Structure Property Relationships ◽  
High Pressure Studies ◽  
Hydrogenation Reactions ◽  
Cerium Intermetallics ◽  
Cerium Compounds

AbstractThe structure–property relationships of CeTX intermetallics with structures other than the ZrNiAl and TiNiSi type are systematically reviewed. These CeTX phases form with electron-poor and electron-rich transition metals (T) and X = Mg, Zn, Cd, Hg, Al, Ga, In, Tl, Si, Ge, Sn, Pb, P, As, Sb, and Bi. The review focusses on the crystal chemistry, the chemical bonding peculiarities, and the magnetic and transport properties. Furthermore 119Sn Mössbauer spectroscopic data, high-pressure studies, hydrogenation reactions and the formation of solid solutions are reviewed. This paper is the third of a series of four reviews on equiatomic intermetallic cerium compounds [Part I: R. Pöttgen, B. Chevalier, Z. Naturforsch. 2015, 70b, 289; Part II: R. Pöttgen, B. Chevalier, Z. Naturforsch. 2015, 70b, 695].

Two Rare Hydroazulene-type Sesquiterpenes from the Roots of Aristolochia yunnanensis

2014 ◽  
Vol 69 (6) ◽  
pp. 742-746 ◽  
Author(s):  
Zhuo Du ◽  
Feng-Ni Wen ◽  
Ji-Ping Zhang ◽  
Jian-Feng Wua ◽  
Fang Liu ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Cell Lines ◽  
Spectroscopic Data ◽  
Cytotoxic Activities ◽  
Moderate Activity ◽  
Electronic Circular Dichroism ◽  
The Third ◽  
The Absolute ◽  
First Time ◽  
Circular Dichroïsm

Two new sesquiterpenes, named aristoyunnolin I (1) and J (2), together with eight known compounds (3 - 10) were isolated from the roots of Aristolochia yunnanensis. Compounds 1 and 2 feature a rare hydroazulene-type sesquiterpene skeleton and represent the third and fourth examples of this kind found in nature. The structures were determined from spectroscopic data, and the absolute configurations of 1 - 3 were assigned by comparing experimental with simulated electronic circular dichroism (ECD) spectra. Compounds 1, 2, 6 - 10 were isolated from this plant for the first time. The cytotoxic activities of 1 - 10 were evaluated against P-388 and A-549 cell lines. Only compounds 4 and 5 showed moderate activity with IC50 values ranging from 12.0 to 18.2 μM.

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