Variability of nitrogen-fixing Frankia on Alnus species

Botany ◽  
2008 ◽  
Vol 86 (5) ◽  
pp. 501-510 ◽  
Author(s):  
John H. Markham

Plants maintain mutualistic symbioses with multiple symbiont genotypes that differ in the benefits they provide. To investigate differences in the effect of nitrogen-fixing Frankia on Alnus species, spore-producing (sp+) nodules from Alnus rubra Bong. and Alnus incana subsp. rugosa (Du Roi) Clausen and non-spore-producing (sp–) nodules from Alnus viridis subsp. crispa (Ait.) Turrill, A. rubra, and A. incana subsp. rugosa were collected from each of four different populations and used to inoculate all three Alnus species. As expected, sp+ Frankia produced significantly more nodules on all three species. However, A. crispa, which normally does not have sp+ nodules in the field, was more susceptible to a high level of infection by sp+ Frankia in general, and by any source of sp+ Frankia in particular, whereas A. incana subsp. rugosa, which has the highest abundance of sp+ in the field, was less susceptible to high levels of infection. This suggests that A. incana subsp. rugosa develops resistance to high levels of infection. The infectivity of an sp+ Frankia source on A. viridis subsp. crispa and A. rubra was positively correlated with the proportion of sp+ nodules on the site it was collected from, suggesting that the variation in the abundance of sp+ in the field is caused by sp+ Frankia with different levels of infectivity. There was no effect of Frankia sources on nodule allocation. Plant growth was positively correlated with the specific nodule mass and the specific nodule activity, and negatively correlated with the nodule number per plant. Sp+ Frankia resulted in significantly smaller plants in A. rubra. While there was no overall sp+ type effect on the growth of A. viridis subsp. crispa, the largest plants always resulted when they were inoculated with sp–, and the smallest with sp+ Frankia. Neither spore type nor inoculum source had any effect on the performance of A. rugosa. These results suggest that Alnus species remain susceptible to infection by both Frankia spore types, but are able to modulate the effectiveness of these spore types when they are the common symbionts in the field.

2018 ◽  
Vol 2 (3) ◽  
pp. 225-246 ◽  
Author(s):  
Qamar Naith ◽  
Fabio Ciravegna

Purpose This paper aims to support small mobile application development teams or companies performing testing on a large variety of operating systems versions and mobile devices to ensure their seamless working. Design/methodology/approach This paper proposes a “hybrid crowdsourcing” method that leverages the power of public crowd testers. This leads to generating a novel crowdtesting workflow Developer/Tester- Crowdtesting (DT-CT) that focuses on developers and crowd testers as key elements in the testing process without the need for intermediate as managers or leaders. This workflow has been used in a novel crowdtesting platform (AskCrowd2Test). This platform enables testing the compatibility of mobile devices and applications at two different levels, high-level (device characteristics) or low-level (code). Additionally, a “crowd-powered knowledge base” has been developed that stores testing results, relevant issues and their solutions. Findings The comparison of the presented DT-CT workflow with the common and most recent crowdtesting workflows showed that DT-CT may positively impact the testing process by reducing time-consuming and budget spend because of the direct interaction of developers and crowd testers. Originality/value To authors’ knowledge, this paper is the first to propose crowdtesting workflow based on developers and public crowd testers without crowd managers or leaders, which light the beacon for the future research in this field. Additionally, this work is the first that authorizes crowd testers with a limited level of experience to participate in the testing process, which helps in studying the behaviors and interaction of end-users with apps and obtains more concrete results.


1996 ◽  
Vol 74 (11) ◽  
pp. 1684-1686 ◽  
Author(s):  
Todd M. Hurd ◽  
Christa R. Schwintzer

Small numbers of cluster roots were found on older roots of Alnus incana ssp. rugosa (speckled alder) shrubs in the field at 2 of 11 sites. Cluster roots also formed infrequently in speckled alder grown hydroponically in complete nutrient solution. No cluster roots formed in solutions lacking P or Fe. Small numbers of cluster roots also formed in Alnus glutinosa grown hydroponically in the presence of P but not in its absence. In contrast, Alnus rubra produced small numbers of cluster roots in the absence of P but not in its presence. No cluster roots formed in Alnus viridis ssp. crispa. Cluster roots are probably not important for nutrient uptake in speckled alder because they form infrequently both in the field and in hydroponic culture. Keywords: Alnus incana ssp. rugosa, Alnus glutinosa, Alnus rubra, Alnus viridis ssp. crispa, cluster roots, proteoid roots.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Haoran Chen ◽  
John Markham

AbstractSmall inreases in CO2 stimulate nitrogen fixation and plant growth. Increasing soil N can inhibit nitrogen fixation. However, no studies to date have tested how nitrogen fixing plants perform under ancient CO2 levels (100 MYA), when nitrogen fixing plants evolved, with different levels of N additions. The aim of this study was to assess if ancient CO2, compared to present, favors nitrogen fixers over a range of soil nitrogen concentrations. Nitrogen fixers (Alnus incana ssp. rugosa, Alnus viridis ssp. crispa, and Alnus rubra) and their close non-nitrogen fixing relatives (Betula pumila, Betula papyrifera, Betula glandulosa) were grown at ancient (1600 ppm) or present (400 ppm) CO2 over a range of soil N levels, equivalent to 0, 10, 50, and 200 kg N ha−1 year−1. The growth of non-N fixing plants increased more than N fixing plants in response to the increasing N levels. When grown at an ancient CO2 level, the N level at which non-nitrogen fixing plant biomass exceeded nitrogen fixing plant biomass was twice as high (61 kg N ha−1 year−1) as the N level when plants were grown at the ambient CO2 level. Specific nodule activity was also reduced with an increasing level of soil N. Our results show there was a greater advantage in being a nitrogen fixer under ancient levels of CO2 compared with the present CO2 level.


2020 ◽  
Author(s):  
Haoran Chen ◽  
John Markham

Abstract Small inreases in CO2 stimulate nitrogen fixation and plant growth. Increasing soil N can inhibit nitrogen fixation. However, no studies to date have tested how nitrogen fixing plants perform under ancient CO2 levels (100 MYA) when nitrogen fixers evolved, with different levels of N additions. The aim of this study was to assess if ancient CO2, compared to present, favors nitrogen fixers over a range of soil nitrogen concentrations. Nitrogen fixers (Alnus incana ssp. rugosa, Alnus viridis ssp. crispa, and Alnus rubra) and their close non-nitrogen fixing relatives (Betula pumila, Betula papyrifera, Betula glandulosa) were grown at ancient CO2 (1600 ppm) or present CO2 (400 ppm) over a range of soil N levels, equivalent to 0, 10, 50, and 200 kg N ha-1 year-1. The growth of non-N fixing plants increased more than N fixing plants in response to the increasing N levels. When grown at an ancient CO2 level, the N level at which non-nitrogen fixing plant biomass exceeded nitrogen fixing plant biomass was twice as high (61 kg N ha-1 y-1) as the N level when plants were grown at an ambient CO2 level. Specific nodule activity was also reduced with an increasing level of soil N. Our results showed there was a greater advantage in being a nitrogen fixer under ancient levels of CO2 compared with the present CO2 level.


2020 ◽  
Vol 16 (2) ◽  
pp. 138-152
Author(s):  
Bingren Zhang ◽  
Chu Wang ◽  
Chanchan Shen ◽  
Wei Wang

Background: Responses to external emotional-stimuli or their transitions might help to elucidate the scientific background and assist the clinical management of psychiatric problems, but pure emotional-materials and their utilization at different levels of neurophysiological processing are few. Objective: We aimed to describe the responses at central and peripheral levels in healthy volunteers and psychiatric patients when facing external emotions and their transitions. Methods: Using pictures and sounds with pure emotions of Disgust, Erotica, Fear, Happiness, Neutral, and Sadness or their transitions as stimuli, we have developed a series of non-invasive techniques, i.e., the event-related potentials, functional magnetic resonance imaging, excitatory and inhibitory brainstem reflexes, and polygraph, to assess different levels of neurophysiological responses in different populations. Results: Sample outcomes on various conditions were specific and distinguishable at cortical to peripheral levels in bipolar I and II disorder patients compared to healthy volunteers. Conclusions: Methodologically, designs with these pure emotions and their transitions are applicable, and results per se are specifically interpretable in patients with emotion-related problems.


2005 ◽  
Vol 24 (s-1) ◽  
pp. 171-193 ◽  
Author(s):  
Michael Gibbins ◽  
Susan A. McCracken ◽  
Steven E. Salterio

Much of what takes place in auditor-client management negotiations occurs in unobservable settings and normally does not result in publicly available archival records. Recent research has increasingly attempted to probe issues relating to accounting negotiations in part due to recent events in the financial world. In this paper, we compare recalls from the two sides of such negotiations, audit partners, and chief financial officers (CFOs), collected in two field questionnaires. We examine the congruency of the auditors' and the CFOs' negotiation recalls for all negotiation elements and features that were common across the two questionnaires (detailed analyses of the questionnaires are reported elsewhere). The results show largely congruent recall: only limited divergences in recall of common elements and features. Specifically, we show a high level of congruency across CFOs and audit partners in the type of issues negotiated, parties involved in resolving the issue, and the elements making up the negotiation process, including agreement on the relative importance of various common accounting contextual features. The analysis of the common accounting contextual features suggests that certain contextual features are consistently important across large numbers of negotiations, whether viewed from the audit partner's or the CFO's perspective, and hence may warrant future study. Finally, the comparative analysis allows us to identify certain common elements and contextual features that may influence both audit partners and CFOs to consider the accounting negotiation setting as mainly distributive (win-lose).


Aerospace ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 61
Author(s):  
Dominik Eisenhut ◽  
Nicolas Moebs ◽  
Evert Windels ◽  
Dominique Bergmann ◽  
Ingmar Geiß ◽  
...  

Recently, the new Green Deal policy initiative was presented by the European Union. The EU aims to achieve a sustainable future and be the first climate-neutral continent by 2050. It targets all of the continent’s industries, meaning aviation must contribute to these changes as well. By employing a systems engineering approach, this high-level task can be split into different levels to get from the vision to the relevant system or product itself. Part of this iterative process involves the aircraft requirements, which make the goals more achievable on the system level and allow validation of whether the designed systems fulfill these requirements. Within this work, the top-level aircraft requirements (TLARs) for a hybrid-electric regional aircraft for up to 50 passengers are presented. Apart from performance requirements, other requirements, like environmental ones, are also included. To check whether these requirements are fulfilled, different reference missions were defined which challenge various extremes within the requirements. Furthermore, figures of merit are established, providing a way of validating and comparing different aircraft designs. The modular structure of these aircraft designs ensures the possibility of evaluating different architectures and adapting these figures if necessary. Moreover, different criteria can be accounted for, or their calculation methods or weighting can be changed.


Author(s):  
yifan yang ◽  
Lorenz S Cederbaum

The low-lying electronic states of neutral X@C60(X=Li, Na, K, Rb) have been computed and analyzed by employing state-of-the-art high level many-electron methods. Apart from the common charge-separated states, well known...


Electronics ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 73
Author(s):  
Francesco Ratto ◽  
Tiziana Fanni ◽  
Luigi Raffo ◽  
Carlo Sau

With the diffusion of cyber-physical systems and internet of things, adaptivity and low power consumption became of primary importance in digital systems design. Reconfigurable heterogeneous platforms seem to be one of the most suitable choices to cope with such challenging context. However, their development and power optimization are not trivial, especially considering hardware acceleration components. On the one hand high level synthesis could simplify the design of such kind of systems, but on the other hand it can limit the positive effects of the adopted power saving techniques. In this work, the mutual impact of different high level synthesis tools and the application of the well known clock gating strategy in the development of reconfigurable accelerators is studied. The aim is to optimize a clock gating application according to the chosen high level synthesis engine and target technology (Application Specific Integrated Circuit (ASIC) or Field Programmable Gate Array (FPGA)). Different levels of application of clock gating are evaluated, including a novel multi level solution. Besides assessing the benefits and drawbacks of the clock gating application at different levels, hints for future design automation of low power reconfigurable accelerators through high level synthesis are also derived.


2021 ◽  
Vol 11 (3) ◽  
pp. 1223
Author(s):  
Ilshat Khasanshin

This work aimed to study the automation of measuring the speed of punches of boxers during shadow boxing using inertial measurement units (IMUs) based on an artificial neural network (ANN). In boxing, for the effective development of an athlete, constant control of the punch speed is required. However, even when using modern means of measuring kinematic parameters, it is necessary to record the circumstances under which the punch was performed: The type of punch (jab, cross, hook, or uppercut) and the type of activity (shadow boxing, single punch, or series of punches). Therefore, to eliminate errors and accelerate the process, that is, automate measurements, the use of an ANN in the form of a multilayer perceptron (MLP) is proposed. During the experiments, IMUs were installed on the boxers’ wrists. The input parameters of the ANN were the absolute acceleration and angular velocity. The experiment was conducted for three groups of boxers with different levels of training. The developed model showed a high level of punch recognition for all groups, and it can be concluded that the use of the ANN significantly accelerates the collection of data on the kinetic characteristics of boxers’ punches and allows this process to be automated.


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