Improvement of Oxidative and Metabolic Parameters by Cellfood Administration in Patients Affected by Neurodegenerative Diseases on Chelation Treatment

Objective. This prospective pilot study aimed at evaluating the effects of therapy with antioxidant compounds (Cellfood, and other antioxidants) on patients affected by neurodegenerative diseases (ND), who displayed toxic metal burden and were subjected to chelation treatment with the chelating agent calcium disodium ethylenediaminetetraacetic acid (CaNa2EDTA or EDTA).Methods. Two groups of subjects were studied: (a) 39 patients affected by ND and (b) 11 subjects unaffected by ND (controls). The following blood parameters were analyzed before and after three months’ treatment with chelation + Cellfood or chelation + other antioxidants: oxidative status (reactive oxygen species, ROS; total antioxidant capacity, TAC; oxidized LDL, oxLDL; glutathione), homocysteine, vitamin B12, and folate.Results. After 3-months’ chelation + Cellfood administration oxLDL decreased, ROS levels were significantly lower, and TAC and glutathione levels were significantly higher than after chelation + other antioxidants treatment, both in ND patients and in controls. Moreover, homocysteine metabolism had also improved in both groups.Conclusions. Chelation + Cellfood treatment was more efficient than chelation + other antioxidants improving oxidative status and homocysteine metabolism significantly in ND patients and controls. Although limited to a small number of cases, this study showed how helpful antioxidant treatment with Cellfood was in improving the subjects’ metabolic conditions.

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EDTA Chelation Therapy in the Treatment of Neurodegenerative Diseases: An Update

Biomedicines ◽

10.3390/biomedicines8080269 ◽

2020 ◽

Vol 8 (8) ◽

pp. 269

Author(s):

Alessandro Fulgenzi ◽

Daniele Vietti ◽

Maria Elena Ferrero

Keyword(s):

Neurodegenerative Diseases ◽

Toxic Metals ◽

Inductively Coupled Plasma ◽

Chelation Therapy ◽

Ethylenediaminetetraacetic Acid ◽

Human Subjects ◽

Toxic Metal ◽

Chelating Agent ◽

Inductively Coupled ◽

Plasma Mass Spectrometry

We have previously described the role played by toxic-metal burdens in the etiology of neurodegenerative diseases (ND). We herein report an updated evaluation of toxic-metal burdens in human subjects affected or not affected by ND or other chronic diseases. Each subject underwent a chelation test with the chelating agent calcium disodium ethylenediaminetetraacetic acid (CaNA2EDTA or EDTA) to identify the presence of 20 toxic metals in urine samples using inductively coupled plasma mass spectrometry. Our results show the constant presence of toxic metals, such as lead, cadmium, cesium, and aluminum, in all examined subjects but the absence of beryllium and tellurium. Gadolinium was detected in patients undergoing diagnostic magnetic resonance imaging. The presence of toxic metals was always significantly more elevated in ND patients than in healthy controls. Treatment with EDTA chelation therapy removes toxic-metal burdens and improves patient symptoms.

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Rationale for the Successful Management of EDTA Chelation Therapy in Human Burden by Toxic Metals

BioMed Research International ◽

10.1155/2016/8274504 ◽

2016 ◽

Vol 2016 ◽

pp. 1-13 ◽

Cited By ~ 25

Author(s):

Maria Elena Ferrero

Keyword(s):

Adverse Effects ◽

Neurodegenerative Diseases ◽

Toxic Metals ◽

Chelation Therapy ◽

Ethylenediaminetetraacetic Acid ◽

Toxic Metal ◽

Chelating Agent ◽

Successful Management ◽

Human Organs ◽

Human Burden

Exposure to environmental and occupational toxicants is responsible for adverse effects on human health. Chelation therapy is the only procedure able to remove toxic metals from human organs and tissue, aiming to treat damage related to acute and/or chronic intoxication. The present review focuses on the most recent evidence of the successful use of the chelating agent ethylenediaminetetraacetic acid (EDTA). Assessment of toxic-metal presence in humans, as well as the rationale of EDTA therapy in cardiovascular and neurodegenerative diseases, is reported.

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Oxidative Status before and after Renal Replacement Therapy: Differences between Conventional High Flux Hemodialysis and on-Line Hemodiafiltration

Nutrients ◽

10.3390/nu11112809 ◽

2019 ◽

Vol 11 (11) ◽

pp. 2809 ◽

Cited By ~ 3

Author(s):

José Alberto Navarro-García ◽

Elena Rodríguez-Sánchez ◽

Jennifer Aceves-Ripoll ◽

Judith Abarca-Zabalía ◽

Andrea Susmozas-Sánchez ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Defense ◽

Oxidized Ldl ◽

Oxidative Status ◽

Dialysis Session ◽

High Flux ◽

Oxidative Markers ◽

Before And After ◽

Total Antioxidant ◽

On Line

Hemodialysis patients experience high oxidative stress because of systemic inflammation and depletion of antioxidants. Little is known about the global oxidative status during dialysis or whether it is linked to the type of dialysis. We investigated the oxidative status before (pre-) and after (post-) one dialysis session in patients subjected to high-flux dialysis (HFD) or on-line hemodiafiltration (OL-HDF). We analyzed carbonyls, oxidized LDL (oxLDL), 8-hydroxy-2′-deoxyguanosine, and xanthine oxidase (XOD) activity as oxidative markers, and total antioxidant capacity (TAC), catalase, and superoxide dismutase activities as measures of antioxidant defense. Indices of oxidative damage (OxyScore) and antioxidant defense (AntioxyScore) were computed and combined into a global DialysisOxyScore. Both dialysis modalities cleared all markers (p < 0.01) except carbonyls, which were unchanged, and oxLDL, which increased post-dialysis (p < 0.01). OxyScore increased post-dialysis (p < 0.001), whereas AntioxyScore decreased (p < 0.001). XOD and catalase activities decreased post-dialysis after OL-HDF (p < 0.01), and catalase activity was higher after OL-HDF than after HFD (p < 0.05). TAC decreased in both dialysis modalities (p < 0.01), but remained higher in OL-HDF than in HFD post-dialysis (p < 0.05), resulting in a lower overall DialysisOxyScore (p < 0.05). Thus, patients on OL-HDF maintain higher levels of antioxidant defense, which might balance the elevated oxidative stress during dialysis, although further longitudinal studies are needed.

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Demineralization, Collagen Modification and Remineralization Degree of Human Dentin after EDTA and Citric Acid Treatments

Materials ◽

10.3390/ma12010025 ◽

2018 ◽

Vol 12 (1) ◽

pp. 25 ◽

Cited By ~ 5

Author(s):

Maria Gandolfi ◽

Paola Taddei ◽

Anna Pondrelli ◽

Fausto Zamparini ◽

Carlo Prati ◽

...

Keyword(s):

Citric Acid ◽

Ethylenediaminetetraacetic Acid ◽

Environmental Scanning Electron Microscopy ◽

Chelating Agent ◽

Environmental Scanning ◽

X Ray ◽

Before And After ◽

Human Dentin ◽

Mineral Components ◽

Mineralization Degree

The aim of the study was to investigate the effects of several decalcifying agents used as irrigant solutions in endodontic treatment on collagen and mineral components of dentin. Coronal dentin discs from five caries-free human third molars with a smear layer were treated for one minute with a chelating solution (1% Ethylenediaminetetraacetic acid (EDTA), 10% EDTA, 17% EDTA, 10% citric acid). Mineralization degree (Ca/N and P/N atomic ratios, IR Iapatite/Iamide II and I1410(carbonate)/I554(phosphate) spectroscopic ratios) and possible collagen rearrangements (collagen infrared (IR) amide II e III shifts) were evaluated by environmental scanning electron microscopy (ESEM)/energy dispersive X-ray spectroscopy (EDX) and IR spectroscopy before and after treatment (T0) and after ageing (T24h and T2m) in simulated body fluid (SBF). At T0, analysis showed that the highest demineralizing effect was achieved using a 10% citric acid solution and 10% EDTA, while the smallest effect was observed when using 17% EDTA. No significant collagen modifications were detected upon treatment with 1% EDTA, while subtle changes were observed after the other treatments. At T24h or T2m, analyses showed the highest remineralization values for 1% EDTA and the lowest for 10% citric acid, mainly at T2m. The samples treated with 17% EDTA showed slight collagen rearrangements upon remineralization. In conclusion, the highest demineralizing effect was observed for 10% EDTA and 10% citric acid. Collagen rearrangement was found for all the treatments except for 1% EDTA. The highest remineralization capability in SBF values was recorded for 1% EDTA and the lowest for 10% citric acid. A slight collagen rearrangement upon remineralization was still present in 17% EDTA-treated samples. Clinical use as a chelating agent in the endodontic therapy of citric acid and concentrated EDTA solutions should be reconsidered.

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EDTA Chelation Therapy for the Treatment of Neurotoxicity

International Journal of Molecular Sciences ◽

10.3390/ijms20051019 ◽

2019 ◽

Vol 20 (5) ◽

pp. 1019 ◽

Cited By ~ 9

Author(s):

Fulgenzi Alessandro ◽

Ferrero Maria Elena

Keyword(s):

Neurodegenerative Diseases ◽

Therapeutic Agent ◽

Chelation Therapy ◽

Ethylenediaminetetraacetic Acid ◽

Organophosphorus Pesticides ◽

Chelating Agent ◽

Oxygen Species ◽

Anesthetic Drugs ◽

Disodium Ethylenediaminetetraacetic Acid ◽

Species Production

Neurotoxicity can be caused by numerous direct agents, of which toxic metals, organophosphorus pesticides, air pollution, radiation and electromagnetic fields, neurotoxins, chemotherapeutic and anesthetic drugs, and pathogens are the most important. Other indirect causes of neurotoxicity are cytokine and/or reactive oxygen species production and adoptive immunotherapy. The development of neurodegenerative diseases has been associated with neurotoxicity. Which arms are useful to prevent or eliminate neurotoxicity? The chelating agent calcium disodium ethylenediaminetetraacetic acid (EDTA)—previously used to treat cardiovascular diseases—is known to be useful for the treatment of neurodegenerative diseases. This review describes how EDTA functions as a therapeutic agent for these diseases. Some case studies are reported to confirm our findings.

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Evaluation of Rock Mechanical Properties Alteration During Matrix Stimulation With Chelating Agents

Journal of Energy Resources Technology ◽

10.1115/1.4032546 ◽

2016 ◽

Vol 138 (3) ◽

Cited By ~ 8

Author(s):

Assad Barri ◽

Mohamed Mahmoud ◽

Salaheldin Elkatatny

Keyword(s):

Mechanical Properties ◽

Elastic Properties ◽

Carbonate Rocks ◽

Chelating Agents ◽

Ethylenediaminetetraacetic Acid ◽

Chelating Agent ◽

Well Stimulation ◽

Before And After ◽

Rock Mechanical Properties ◽

Carbonate Formations

Well stimulation using acidic solutions is widely used to treat carbonate formations. The acidic fluids remove the near-wellbore damage and create channels around the wellbore by dissolving fraction of the carbonate rocks. Many stimulation fluids have been used such as hydrochloric acid (HCl) acid, organic acids, and chelating agents to stimulate carbonate reservoirs. Wormholes that are created by these fluids are very effective and will yield negative skin values and this will enhance the well productivity. In addition to the wormhole creation, the diffusion of these fluids inside the pores of the rock may create significant and permanent changes in the rock mechanical properties. These changes can eventually lead to weakening the rock strength, which may lead to future formation damage due to the wellbore instability. In this paper, the effect of ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA) chelating agents on the carbonate rocks elastic properties was investigated. The effect of wormholes created by chelating agent on the rock mechanical properties was investigated. Computed tomography (CT) scan and acoustic measurements were conducted on the core samples before and after matrix stimulation treatments. Experimental results showed that the mechanical properties of strong rocks such as Indiana limestone (IL) cores were not affected when chelating agents were used to stimulate those cores. On the other hand, less strong rocks such as Austin chalk (AC) show significant alteration on the rock elastic properties when chelating agents were used as stimulation fluids.

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Atomic Force Microscopy of Removal of Dentin Smear Layers

Microscopy and Microanalysis ◽

10.1017/s1431927607070419 ◽

2007 ◽

Vol 13 (4) ◽

pp. 245-250 ◽

Cited By ~ 10

Author(s):

Luiz Henrique Carvalho Batista ◽

José Ginaldo da Silva Júnior ◽

Milton Fernando Andrade Silva ◽

Josealdo Tonholo

Keyword(s):

Atomic Force Microscopy ◽

Ethylenediaminetetraacetic Acid ◽

Chelating Agent ◽

Root Surface ◽

Smear Layer ◽

Alternative Methods ◽

Force Microscopy ◽

Atomic Force ◽

Afm Analysis ◽

Before And After

The regular periodontal practice of scaling and root planing produces a smear layer on the root surface that is detrimental to the readhesion of tissues during subsequent regeneration therapy. Although it has been demonstrated that gels containing the chelating agent ethylenediaminetetraacetic acid (EDTA) can assist in the removal of this contaminating layer, no quantitative method is yet available by which to evaluate the efficiency of the treatment. In this article, the power of atomic force microscopy (AFM) as a technique for monitoring and mapping the surfaces of dentinal roots is demonstrated. Roughness parameters of teeth that had been scaled and root planed were determined from AFM images acquired both before and after treatment with EDTA. The results confirmed that EDTA is an efficient cleaning agent and that dentinal samples free from a smear layer are significantly rougher than the same samples covered by a contaminating layer. AFM analysis is superior to alternative methods involving scanning electron microscopy because the same sample section can be analyzed many times, thus permitting it to be used as both the control and the treatment surface.

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Effect of Ca-EDTA on performance, blood parameters and muscle color of grain-fed Holstein veal calves

Canadian Journal of Animal Science ◽

10.4141/cjas92-005 ◽

1992 ◽

Vol 72 (1) ◽

pp. 41-50 ◽

Cited By ~ 3

Author(s):

Serge A. Pommier ◽

Claire Vinet ◽

Bertrand Lachance

Keyword(s):

Ethylenediaminetetraacetic Acid ◽

Chelating Agent ◽

Blood Parameters ◽

Disodium Salt ◽

Veal Calves ◽

Time Of Application ◽

Time Period ◽

Blood Haemoglobin ◽

Fast Cooling ◽

Muscle Color

Sixty-four grain-fed male Holstein veal calves were allotted to treatments and were fed three levels of the calcium chelate of the disodium salt of ethylenediaminetetraacetic acid (Ca-EDTA) in their diet (0 mg, 30 mg and 60 mg of Ca-EDTA mg−1 of dietary Fe) for two time periods prior to slaughter (4 and 8 wk). Carcasses were then allotted to one of two cooling rates (slow and fast). Neither level of Ca-EDTA nor time period affected feed intake, ADG or feed efficiency. Regardless of time of application, the chelating agent significantly (P < 0.01) reduced serum Fe concentrations within 1 wk of initiation of treatment and blood haemoglobin concentrations within 2 wk of initiation of treatment. Liver Fe concentrations were significantly reduced (P < 0.05) and liver Cu concentrations were significantly (P < 0.01) increased by both levels of Ca-EDTA. The digestibility of feed DM or N was not significantly (P > 0.05) affected by treatments. The inclusion of Ca-EDTA for 8 wk prior to slaughter significantly (P < 0.05) lightened the color of the pectoralis. However, no significant dose response between the 30-mg and 60-mg treatments was observed. Compared to controls, the 4-wk treatment was not effective in improving the color of the meat. The fast cooling rate significantly (P < 0.05) lightened the appearance of the pectoralis muscle. In conclusion, EDTA effectively lightened muscle color without affecting animal performance. Key words: Veal, grain, EDTA, digestibility, chelator, color

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Melatonin Improves Lipid Profile and Ameliorates Lipid Peroxidation in Patients with Chronic Kidney Disease.

Al Mustansiriyah Journal of Pharmaceutical Sciences ◽

10.32947/ajps.18.01.0360 ◽

2018 ◽

pp. 38-45

Keyword(s):

Lipid Peroxidation ◽

Placebo Group ◽

Lipid Profile ◽

Kidney Diseases ◽

Oxidized Ldl ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Before And After ◽

Inhibit Lipid Peroxidation ◽

Controlled Clinical Study

Dyslipidemia and oxidative modifications of lipid are frequently associated in patients with chronic kidney diseases (CKD) and considered the most important risk factors for cardiovascular events. Melatonin is a well-known potent antioxidant and has beneficial effect on lipid metabolism. the study was designed to evaluate if Melatonin could improve lipid profile and ameliorates lipid peroxidation. This single blind placebo controlled clinical study carried out on 41 patients with CKD who were randomized into two groups, control groups (n=20) those who received placebo cap and melatonin group those who received 5mg melatonin (n=21). Lipid profile [total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C)] and parameters of lipid peroxidation [oxidized LDL (oxLDL) and malondialdehyde (MDA) were measured before and after 12 weeks of the treatment. After 12 weeks of treatment, melatonin significantly increased HDL-C and decreased LDL-C compared to the initial value. The elevation in HDL-C and reduction in LDL-C were significantly different from that in placebo group. Also, both oxLDL and MDA levels significantly lowered by melatonin compared to the baseline and to the placebo group. Collectively, the results of our study showed that melatonin has advantageous effect on lipid profile and inhibit lipid peroxidation in patients with CKD.

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