Assessment of Serum Enzymatic Antioxidant Levels in Patients with Recurrent Aphthous Stomatitis: A Case Control Study

Background and Aim. Recurrent aphthous stomatitis (RAS) is a common oral mucosal disorder characterized by recurrent, painful oral aphthae. Despite extensive research, the exact etiology of RAS remains elusive. Recently oxidant-antioxidant imbalance of the body has been implicated in the pathogenesis of recurrent aphthous stomatitis. Thus, the aim of the study was to evaluate the enzymatic antioxidant levels in patients with recurrent aphthous stomatitis. Materials and Methods. The serum levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were measured in 30 patients with recurrent aphthous stomatitis and compared to the control group, which included 30 healthy subjects. Student’s t-test was performed for statistical evaluation. Results. The mean levels of superoxide dismutase (130.2 ± 15.94 U/mL) and glutathione peroxidase (3527.93 ± 488.32 U/L) were found to be significantly lower in study group as compared to control group (211.9 ± 20.93 U/mL, 8860.93 ± 1105.31 U/L, resp.) (P=0.000) while level of catalase in study group was significantly higher when compared to control group (10981.00 ± 1018.07 U/mL versus 9764.00 ± 1621.19 U/mL) (P=0.000). Conclusion. Enzymatic antioxidant system is impaired in recurrent aphthous stomatitis patients and seems to play a crucial role in its pathogenesis.

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Effects of Three Types of Massage on Serum Levels of Malondialdehyde, Superoxide Dismutase and Glutathione Peroxidase After One Session of Exhaustive Exercise in Female Futsal Players

Complementary Medicine Journal ◽

10.32598/cmja.10.4.631.2 ◽

2021 ◽

Vol 10 (4) ◽

pp. 328-339

Author(s):

Bahare Heydari ◽

◽

Mohsen Ghofrani ◽

Mohammad Ebrahim Bahram ◽

◽

...

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Glutathione Peroxidase ◽

Recovery Period ◽

Serum Levels ◽

The Body ◽

Exhaustive Exercise ◽

Oxidative Stress Marker ◽

Control Group ◽

Significant Difference

Objective: The production of reactive oxygen species in exercise causes oxidative stress which disturbs the balance of oxidants and antioxidants, causing destructive effects on cells. The present study aims to investigate the effect of three types of massage (Swedish, Russian, Thai) on serum levels of Malondialdehyde (MDA), Glutathione Peroxidase (GPX) and Superoxide Dismutase (SOD) following one session of exhaustive exercise. Methods: This quasi-experimental study was conducted on 48 female futsal players aged 17-22 years in Zahedan, Iran who were selected using a purposive sampling method, and randomly divided into four groups of Swedish massage (Long strokes with pressing and tapping using hands), Russian massage (Medium to high pressure), Thai massage (Pressure to certain parts of the body) and Control. The exercise program was based on Bruce protocol. Serum levels of MDA, GPX and SOD were measured by before and immediately after exercise and after massage. Data analysis was performed using repeated measures ANOVA, considering a significance level of P≤0.05. Results: In all three types of massage, there was a significant decrease in serum level of MDA (0.22±0.08), and a significant increase in GPX (1.84±0.46) and SOD (10.02±2.86) levels after exhaustive (P=0.001). No significant difference was observed in the control group. Conclusion: It seems that Russian, Thai, and Swedish types of massage can affect the serum levels of the MDA (as an oxidative stress marker) and the antioxidant enzymes of GPX and SOD during the post-exercise recovery period.

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Mannose-binding lectin serum levels and (Gly54asp) gene polymorphism in recurrent aphthous stomatitis: A case-control study

International Journal of Immunopathology and Pharmacology ◽

10.1177/20587384211064454 ◽

2021 ◽

Vol 35 ◽

pp. 205873842110644

Author(s):

Shereen A Baioumy ◽

Shaimaa H Fouad ◽

Shaimaa A Abdalgeleel ◽

Ahmed A Baiomy ◽

Dina E Sallam ◽

...

Keyword(s):

Case Control Study ◽

Serum Levels ◽

Case Control ◽

Recurrent Aphthous Stomatitis ◽

Mannose Binding Lectin ◽

Aphthous Stomatitis ◽

Control Group ◽

Mannose Binding ◽

Binding Lectin ◽

Control Study

Objectives: Dysregulation of the immune response appears to play a significant role in recurrent aphthous stomatitis (RAS) development. The main objective of this case–control study is to investigate the blood levels of mannose-binding lectin (MBL) and the frequency of the MBL2 gene (gly54asp) polymorphism in RAS patients, including 40 RAS patients and 40 healthy controls. Methods: Serum MBL levels were determined by ELISA, while the PCR-restriction fragment length polymorphism was used in MBL2 genotyping. Results: The median serum MBL level was significantly lower in the RAS group than in the control group (975 ng/mL (545–1320) vs. 1760 ng/mL (1254–2134); p≤ 0.001). The MBL levels were significantly lower in the BB genotype, whereas they were significantly higher in the wild type AA with a median of 525 and 1340 ng/mL, respectively ( p =0.005). The B allele was expressed in significantly higher percentages of RAS patients than in controls. There was no significant association between MBL serum levels ( p=0.685) or MBL2 codon 54 genotypes ( p=0.382) with the type of ulcers. Conclusion: There was an association between low MBL serum levels and the variant allele B of the MBL2 (gly54asp) gene, and the susceptibility to RAS. As a result, potential novel therapeutic options for RAS patients with MBL deficiency should be investigated.

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Detection of HSV1 and HSV2 viruses among Iraqi patients with recurrent Stomatitis Aphthous

Iraqi Dental Journal ◽

10.46466/idj.v40i1.134 ◽

2018 ◽

Vol 40 (1) ◽

pp. 19-24

Author(s):

Eman A Al-Hasnawi

Keyword(s):

Family History ◽

Recurrent Aphthous Stomatitis ◽

Aphthous Stomatitis ◽

Control Group ◽

Study Group ◽

Elisa Method ◽

Healthy Control ◽

Simplex Virus ◽

Positive Results ◽

Hsv 1

Background: Recurrent aphthous stomatitis (RAS) is a global oral case that stay incompletely understood yet. It was classified into three main types of minor, major or herpetiform, based on clinical features, but the exact causative agent is unknown, therefore treatments are primarily should be experimental and aimed at decrease symptoms rather than remedy or prevention. The aim of study is to detection of Herpes simplex virus-1 and Herpes simplex virus-2 in saliva of patients with recurrent aphthous stomatitis (RAS) as a causative agents of the disease by the enzyme-linked immunosorbent assay (ELISA) assay, and correlation between positive results and finding of age, gender, and family history. Materials and Methods: Study group of (40) saliva samples were taken from 40 patients with RAS lesions included 15 male and 25 female, with mean age at (34) years, at range about (18-55) years , and healthy control group were (20) healthy volunteers were suffered from oral traumatic ulcers, consist women (13), men (7), mean age (33) years in range (18-55). for detection of HSV viruses types that may causes this disease, and healthy control group were (20) healthy volunteers were suffered from oral traumatic ulcers, ELISA method used for diagnosis HSV-1 and HSV-2, from saliva samples of the patients with (RAS) with correlation between positive results and finding of age, gender, and family history. Results: Diagnostic specimens were consisted of two groups, study group of (40) saliva samples taken from 40 patients with RAS lesions. The results of ELISA to detect HSV had high significant (17): 42.5% from (40) patients with (RAS), HSV-1: (13)32.5%, and HSV-2: (4)10%. Conclusion: This high number of RAS cases by HSV, and presence of HSV-2 in patients saliva in this study acted important cause of prevalence of this disease in Iraq, were in age group (18-25) mostly in female especially in those with family history of infection. Keywords: Recurrent Aphthous Stomatitis (RAS), Aphthous ulcer, HSV-1&2 viruses, ELISA method

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Characteristic Features of Biochemical Indicators of Mixed Saliva in Patients with Chronic Recurrent Aphthous Stomatitis

Journal of Advances in Medicine and Medical Research ◽

10.9734/jammr/2019/v31i330289 ◽

2019 ◽

pp. 1-7

Author(s):

Nazira N. Khabibova

Keyword(s):

Suppressor Cells ◽

Recurrent Aphthous Stomatitis ◽

The Body ◽

Aphthous Stomatitis ◽

Medical Institute ◽

Control Group ◽

Leading Role ◽

Characteristic Features ◽

Severity Of The Disease ◽

Mixed Saliva

Chronic recurrent aphthous stomatitis (CRAC) is a chronic disease of the oral mucosa, characterized by periodic remissions and exacerbations with the eruption of aft. According to WHO, it affects up to 20% of the population. Currently, most scientists are inclined to the leading role of the immune system in the pathogenesis of the disease. It has been established that with increasing severity of the disease T-suppression of immunity increases, which is characterized by a decrease in the number of T-lymphocytes and their functional activity. The increase in the number of T-suppressor cells accompanied by a decrease in the number of T-helper cells. The severity and duration of the disease correspond to the severity of the sensitization of the body with these antigens. The work was carried out based on scientific-educational practical dental center at the Bukhara State Medical Institute of Therapeutic Dentistry. A clinical examination of 67 patients aged 25–35 years with chronic recurrent aphthous stomatitis was conducted, 14 healthy individuals were included in the control group.

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Assessment of Serum Concentrations of Ghrelin, Obestatin, Omentin-1, and Apelin in Children with Type 1 Diabetes

BioMed Research International ◽

10.1155/2016/8379294 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5 ◽

Cited By ~ 4

Author(s):

Agnieszka Polkowska ◽

Izabela Szczepaniak ◽

Artur Bossowski

Keyword(s):

Insulin Resistance ◽

Type 1 Diabetes ◽

Disease Duration ◽

Serum Levels ◽

The Body ◽

Control Group ◽

Study Group ◽

Newly Diagnosed ◽

Serum Concentrations

The increasing knowledge on the functions of gastric peptides and adipokines in the body allows the assumption of their major role linking the process of food intake, nutritional status, and body growth, largely through the regulation of glucose metabolism and insulin resistance. The aim of the study was the assessment of serum levels of selected gastric peptides and adipocytokines in children with type 1 diabetes, with respect to the disease duration. The study involved 80 children aged 4–18 years (M/F -37/43). Children with type 1 diabetes (n=46) were compared to the control group (n=34). The study group was divided into 4 subgroups: (I) patients with newly diagnosed type 1 diabetes, after an episode of ketoacidosis (n=10), (II) patients with type 1 diabetes of duration no longer than 5 years (n=9), (III) patients with 5 to 10 years of DT1 (n=20), and (IV) patients with type 1 diabetes of duration longer than 10 years (n=7). The concentrations of gastric peptide and adipocytokines across all subgroups were lower than in the control group. The differences were statistically significant (p<0.0001), which may be of importance in the development of the disease complications.

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Utility of dermatoglyphics as a non-invasive diagnostic tool in recurrent aphthous stomatitis

Archives of Oral Research ◽

10.7213/archives.08.003.ac03 ◽

2012 ◽

Vol 8 (3) ◽

Author(s):

Suwarna Dangore-Khasbage ◽

Shirish S. Degwekar ◽

Rahul R. Bhowate ◽

Vidya K. Lohe ◽

Dharmaraj Tamgire

Keyword(s):

Genetic Disorders ◽

Medical Science ◽

Recurrent Aphthous Stomatitis ◽

Aphthous Stomatitis ◽

Control Group ◽

Study Group ◽

Ridge Count ◽

Non Invasive ◽

Significant Difference ◽

And Control

Introduction: Dermatoglyphics is an advancing branch of medical science in which the dermal ridge patterns are studied and used in prediction of genetic disorders. Objective: To assess the usefulness of dermatoglyphics, as a non-invasive early predicator in RAS, since genetics plays a role in both. Materials and methods: This case control study comprised of 40 patients with recurrent aphthous stomatitis compared to 30 control subjects of similar age and sex. All subjects were investigated for their dermatoglyphic patterns of both hands, which were obtained by smearing the ink uniformly over the palm and fingers and pressing the hands firmly against the good quality paper. Qualitative analysis of prints was done for arches, loops and whorls and quantitative analysis included Total Finger Ridge Count (TFRC) and atd angle. Fisher’s Exact Tests were used to evaluate the significance between recurrent aphthous stomatitis and the dermatoglyphic features. Results: Among various dermatoglyphic parameters analyzed, recurrent aphthous stomatitis patients showed 9% arches, 62% loops and 29% whorls as the finger ridge configuration. In comparing the results between the two groups, the frequency of arches was observed more in study group while composite whorl and the nar pattern were more frequent in control group, the results were statistically significant (p < 0.05). In addition to this, study group patients demonstrated higher frequency of total finger ridge count, and control group showed higher frequency for atd angle on both hands, statistically significant difference is observed (p < 0.05). Conclusion: The study found significant correlation between palmar dermatoglyphics and recurrent aphthous stomatitis, suggesting that genetics is one of the host risk factor associated with the latter, and could aid in early detection of the disease.

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Serum Thiol-Disulphide Levels in Epileptic Pediatric Patients

Combinatorial Chemistry & High Throughput Screening ◽

10.2174/1386207322666190305135043 ◽

2019 ◽

Vol 22 (1) ◽

pp. 65-68

Author(s):

Halit Halil ◽

Nilden Tuygun ◽

Erhan Aksoy ◽

Ozcan Erel ◽

Can Demir Karacan

Keyword(s):

Oxidative Stress ◽

Antioxidant Status ◽

Pediatric Patients ◽

Serum Levels ◽

The Body ◽

Control Group ◽

Study Group ◽

Total Thiol ◽

Stress Markers ◽

Meaningful Relationship

Background: Epilepsy is a serious clinical condition characterized by recurrent seizures. Oxidative stress plays an important role in the etio-pathogenesis of epilepsy. Measurements of serum thiol and disulfide levels were used to evaluate the antioxidant status of the body. Objective: The aim of this study was to determine serum levels of thiol and disulfide in epileptic pediatric patients. Methods: Ninety patients, 54 epilepsy and 36 controls were included in the study. Serum levels of native thiol total thiol and disulfide were measured and disulfide/native, disulfide / total thiol and native thiol/ total thiol ratios were calculated. Hence, the ratios of disulfide/ native thiol, disulfide / total thiol and native thiol/ total thiol were calculated. Results: Serum levels of native thiol, total thiol and disulfide were significantly lower in the epilepsy group than the control group. The ratio of disulfide/native thiol and disulfide / total thiol were significantly higher in the study group than the control group. As well as, the native thiol / total thiol ratio was lower in the epilepsy group than the control group. Native thiol, total thiol and disulfide were significantly lower in the epilepsy group who were taking anti-epileptic drugs than those who were not taking anti-epileptic drugs. Conclusion: We demonstrated a meaningful relationship between oxidative stress markers and epilepsy in pediatric patients.

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State of the enzyme link of antioxidant protection in boys with hypoandrogenism

Problems of Uninterrupted Medical Training and Science ◽

10.31071/promedosvity2020.04.032 ◽

2020 ◽

Vol 40 (4) ◽

pp. 32-36

Author(s):

L. K. Parkhomenko ◽

◽

L. A. Strashok ◽

S. I. Turchina ◽

G. V. Kosovtsova ◽

...

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Free Radical ◽

Glutathione Peroxidase ◽

Free Radical Oxidation ◽

Conjugated Dienes ◽

Delayed Puberty ◽

Control Group ◽

Antioxidant Protection ◽

Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

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Peripheral levels of superoxide dismutase and glutathione peroxidase in youths in ultra-high risk for psychosis: a pilot study

CNS Spectrums ◽

10.1017/s1092852917000803 ◽

2017 ◽

Vol 24 (03) ◽

pp. 333-337 ◽

Cited By ~ 5

Author(s):

Maiara Zeni-Graiff ◽

Adiel C. Rios ◽

Pawan K. Maurya ◽

Lucas B. Rizzo ◽

Sumit Sethi ◽

...

Keyword(s):

Oxidative Stress ◽

At Risk ◽

Superoxide Dismutase ◽

High Risk ◽

Glutathione Peroxidase ◽

Healthy Control Group ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Ultra High Risk ◽

Healthy Control

IntroductionOxidative stress has been documented in chronic schizophrenia and in the first episode of psychosis, but there are very little data on oxidative stress prior to the disease onset.ObjectiveThis work aimed to compare serum levels of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in young individuals at ultra-high risk (UHR) of developing psychosis with a comparison healthy control group (HC).MethodsThirteen UHR subjects and 29 age- and sex-matched healthy controls (HC) were enrolled in this study. Clinical assessment included the Comprehensive Assessment of At-Risk Mental States (CAARMS), the Semi-Structured Clinical Interview for DSM-IV Axis-I (SCID-I) or the Kiddie-SADS-Present and Lifetime Version (K-SADS-PL), and the Global Assessment of Functioning (GAF) scale. Activities of SOD and GPx were measured in serum by the spectrophotometric method using enzyme-linked immunosorbent assay kits.ResultsAfter adjusting for age and years of education, there was a significant lower activity of SOD and lower GPX activity in the UHR group compared to the healthy control group (rate ratio [RR]=0.330, 95% CI 0.187; 0.584, p<0.001 and RR=0.509, 95% CI 0.323; 0.803, p=0.004, respectively). There were also positive correlations between GAF functioning scores and GPx and SOD activities.ConclusionOur results suggest that oxidative imbalances could be present prior to the onset of full-blown psychosis, including in at-risk stages. Future studies should replicate and expand these results.

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Glutathione peroxidase (EC 1.11.1.9) and superoxide dismutase (EC 1.15.1.1) activities in riboflavin-deficient rats infected with Plasmodium berghei malaria

British Journal Of Nutrition ◽

10.1079/bjn19980048 ◽

1998 ◽

Vol 79 (3) ◽

pp. 305-309 ◽

Cited By ~ 12

Author(s):

D. A. Adelekan ◽

D. I. Thurnham

Keyword(s):

Superoxide Dismutase ◽

Glutathione Peroxidase ◽

Plasmodium Berghei ◽

Control Group ◽

Riboflavin Deficiency ◽

Sod Activity ◽

Parasite Protein ◽

Riboflavin Deficient ◽

Gpx Activity ◽

Deficient Group

Riboflavin deficiency interferes with the growth and multiplication of malaria parasites as well as the host response to malaria. The objective of the present work was to determine the effects of riboflavin deficiency on erythrocyte glutathione peroxidase (EC1.11.1.9; GPx) and superoxide dismutase (EC1.15.1.1; SOD) in rats infected withPlasmodium bergheimalaria. Riboflavin in its co-enzyme form, FAD, is required by glutathione reductase (EC1.6.4.1) to regenerate GSH and GSH is an important cellular antioxidant both in its own right and also as a substrate for the enzyme GPx. Weanling rats were deprived of riboflavin for 8 weeks before intraperitoneal injection of 1 × 106P. bergheiparasites. Control animals were weight-matched to the respective riboflavin-deficient group. At 10d post-infection, parasite counts were higher in the weight-matched control group than the riboflavin-deficient group (P= 0.004). GPx activity was higher in erythrocytes of rats parasitized withP. bergheithan comparable non-infected rats regardless of riboflavin status (P< 0.05). As mature erythrocytes do not synthesize new protein, the higher GPx activities were probably due to the presence of the parasite protein. In erythrocytes from riboflavin-deficient rats, GPx activity tended to be lower than in those rats fed on diets adequate in riboflavin (weight-matched controls) whether parasitized or not, but the difference was not significant. Neither riboflavin deficiency nor malaria had any effect on erythrocyte SOD activity. It was concluded that riboflavin deficiency has no marked effect on erythrocyte GPx or SOD activity in the rat.

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