Serum Thiol-Disulphide Levels in Epileptic Pediatric Patients

2019 ◽  
Vol 22 (1) ◽  
pp. 65-68
Author(s):  
Halit Halil ◽  
Nilden Tuygun ◽  
Erhan Aksoy ◽  
Ozcan Erel ◽  
Can Demir Karacan
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Pediatric Patients ◽  
Serum Levels ◽  
The Body ◽  
Control Group ◽  
Study Group ◽  
Total Thiol ◽  
Stress Markers ◽  
Meaningful Relationship

Background: Epilepsy is a serious clinical condition characterized by recurrent seizures. Oxidative stress plays an important role in the etio-pathogenesis of epilepsy. Measurements of serum thiol and disulfide levels were used to evaluate the antioxidant status of the body. Objective: The aim of this study was to determine serum levels of thiol and disulfide in epileptic pediatric patients. Methods: Ninety patients, 54 epilepsy and 36 controls were included in the study. Serum levels of native thiol total thiol and disulfide were measured and disulfide/native, disulfide / total thiol and native thiol/ total thiol ratios were calculated. Hence, the ratios of disulfide/ native thiol, disulfide / total thiol and native thiol/ total thiol were calculated. Results: Serum levels of native thiol, total thiol and disulfide were significantly lower in the epilepsy group than the control group. The ratio of disulfide/native thiol and disulfide / total thiol were significantly higher in the study group than the control group. As well as, the native thiol / total thiol ratio was lower in the epilepsy group than the control group. Native thiol, total thiol and disulfide were significantly lower in the epilepsy group who were taking anti-epileptic drugs than those who were not taking anti-epileptic drugs. Conclusion: We demonstrated a meaningful relationship between oxidative stress markers and epilepsy in pediatric patients.

Role of Ischemia Modified Albumin Serum Levels as an Oxidative Stress Marker in Children with Diabetic Ketoacidosis

2019 ◽  
Vol 22 (8) ◽  
pp. 577-581
Author(s):  
Mutlu U. Yazıcı ◽  
Ganime Ayar ◽  
Senay Savas-Erdeve ◽  
Ebru Azapağası ◽  
Salim Neşelioğlu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Intensive Care Unit ◽  
Intensive Care ◽  
Diabetic Ketoacidosis ◽  
Pediatric Patients ◽  
Serum Levels ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Study Group ◽  
Ischemia Modified Albumin

Aim and Objective: Ischemia modified albumin (IMA) is a biomarker that has been introduced recently for use in the evaluation of oxidative stress. The aim of this study was to measure the ischemia modified albumin serum levels in pediatric patients with diabetic ketoacidosis (DKA) during acidosis and after the patient recovered from acidosis and to compare these with the control group. Materials and Methods: Pediatric patients with Type I diabetes mellitus (T1DM) who were admitted to the pediatric intensive care unit with the diabetic ketoacidosis were assigned as the study group and healthy children who were admitted to the outpatient clinic and decided as healthy after clinic and laboratory evaluation were selected as the control group. IMA and adjusted IMA levels were evaluated in the blood samples from the control group and the study group when admitted first time to the intensive care unit during the acidosis period (DKA before treatment, DKA-BT), and after recovering from acidosis (DKA after treatment, DKA-AT). Results: A total of 24 pediatric patients with diabetic ketoacidosis and 30 healthy control children matching age and sex were included in the current study. The albumin levels in pediatric patients with T1DM during DKA-BT were higher than the albumin levels after acidosis (4.101±0.373, 3.854±0.369 g/dL, respectively) (p<0.05). However, there was no significant difference when these values were compared to the control group. Mean values of IMA and Adj-IMA were statistically higher in DKAAT compared to the control group (0.748±0.150 vs 0.591±0.099, p< 0.001; 0.708±0.125 vs 0.607±0.824, p< 0.001, respectively). IMA and adjusted IMA levels measured after recovered from acidosis were significantly higher compared to the level of IMA during DKA (0.748±0.150 vs 0.606±0.105 as absorbance unit, p<0.001; 0.708±0.125 vs 0.625±0.100, p<0.05, respectively). Conclusion: In children with T1DM, even though acidosis recovered following the treatment in diabetic ketoacidosis, which is an oxidative stress marker, the ischemia modified albumin levels and adjusted ischemia modified albumin levels were high.

Dynamic thiol/disulphide homeostasis metrics as a risk factor for peripheral arterial disease

Vascular ◽  
2020 ◽  
pp. 170853812094724
Author(s):  
Ufuk Turan Kursat Korkmaz ◽  
Ahmet Yuksel ◽  
Ayhan Cetinkaya ◽  
Yusuf Velioglu ◽  
Erhan Renan Ucaroglu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Risk Factor ◽  
Peripheral Arterial Disease ◽  
Laboratory Data ◽  
Arterial Disease ◽  
Control Group ◽  
Study Group ◽  
Total Thiol ◽  
Peripheral Arterial ◽  
Study Participants

Objective To examine dynamic thiol/disulphide homeostasis metrics as a novel risk factor of oxidative stress in patients with peripheral arterial disease. Methods One hundred patients with lower extremity peripheral arterial disease (a study group) and 100 control subjects were included in this prospective case–control study. Participants’ baseline clinical characteristics and laboratory data including some oxidant/antioxidant status parameters such as albumin, ferroxidase and myeloperoxidase, and thiol/disulphide homeostasis parameters such as native thiol, total thiol and disulphide, as well as native thiol/total thiol, disulphide/native thiol and disulphide/total thiol ratios were all recorded and then compared between the groups. Results Mean albumin and ferroxidase, and median myeloperoxidase levels were found to be significantly higher in patients with the peripheral arterial disease than in control group ( p = 0.045, p = 0.000 and p = 0.000, respectively). Mean native thiol and total thiol, and median disulphide levels were found to be significantly lower in the study group as compared with the control group ( p = 0.000, p = 0.000 and p = 0.037, respectively). According to the results of logistic regression analysis, systolic blood pressure, ferroxidase and myeloperoxidase levels were detected to be the independent predictors of peripheral arterial disease. Conclusion Our report is the first one in the literature investigating dynamic thiol/disulphide homeostasis metrics as a novel risk factor of oxidative stress in peripheral arterial disease. Dynamic thiol/disulphide homeostasis metrics may be used as a valuable risk factor of oxidative stress in patients with the peripheral arterial disease since it is readily available, easily calculated and relatively cheap.

scholarly journals Effects of Three Types of Massage on Serum Levels of Malondialdehyde, Superoxide Dismutase and Glutathione Peroxidase After One Session of Exhaustive Exercise in Female Futsal Players

2021 ◽  
Vol 10 (4) ◽  
pp. 328-339
Author(s):  
Bahare Heydari ◽  
◽  
Mohsen Ghofrani ◽  
Mohammad Ebrahim Bahram ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Recovery Period ◽  
Serum Levels ◽  
The Body ◽  
Exhaustive Exercise ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Significant Difference

Objective: The production of reactive oxygen species in exercise causes oxidative stress which disturbs the balance of oxidants and antioxidants, causing destructive effects on cells. The present study aims to investigate the effect of three types of massage (Swedish, Russian, Thai) on serum levels of Malondialdehyde (MDA), Glutathione Peroxidase (GPX) and Superoxide Dismutase (SOD) following one session of exhaustive exercise. Methods: This quasi-experimental study was conducted on 48 female futsal players aged 17-22 years in Zahedan, Iran who were selected using a purposive sampling method, and randomly divided into four groups of Swedish massage (Long strokes with pressing and tapping using hands), Russian massage (Medium to high pressure), Thai massage (Pressure to certain parts of the body) and Control. The exercise program was based on Bruce protocol. Serum levels of MDA, GPX and SOD were measured by before and immediately after exercise and after massage. Data analysis was performed using repeated measures ANOVA, considering a significance level of P≤0.05. Results: In all three types of massage, there was a significant decrease in serum level of MDA (0.22±0.08), and a significant increase in GPX (1.84±0.46) and SOD (10.02±2.86) levels after exhaustive (P=0.001). No significant difference was observed in the control group. Conclusion: It seems that Russian, Thai, and Swedish types of massage can affect the serum levels of the MDA (as an oxidative stress marker) and the antioxidant enzymes of GPX and SOD during the post-exercise recovery period.

scholarly journals Rosuvastatin Affects Tracheal Responsiveness, Bronchoalveolar Lavage Inflammatory Cells, and Oxidative Stress Markers in Hyperlipidemic and Asthmatic Rats

2020 ◽  
Author(s):  
Saeideh Saadat ◽  
Amin Mokhtari-Zaer ◽  
Mousa-Al-Reza Hadjzadeh ◽  
Mohammad Hossein Boskabady
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Bronchoalveolar Lavage ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Total Thiol ◽  
Stress Markers ◽  
Thiol Content ◽  
Wbc Count ◽  
And Oxidative Stress

Statins provide greater protection than predicted from just cholesterol-lowering effects, which is possibly mediated by other pleiotropic actions. This study aimed to examine the possible interaction effect of asthma on lipid profiles and evaluate the effect of rosuvastatin treatment on asthma. The animals were assigned into (1) control, (2) asthmatic, (3) hyperlipidemic, (4) asthmatic-hyperlipidemic, (5) rosuvastatin (40 mg/kg/day intraperitoneally, for 3 weeks)-treated asthmatic, (6) rosuvastatin-treated hyperlipidemic and (7) rosuvastatin-treated asthmatic-hyperlipidemic groups. Tracheal responsiveness to methacholine and ovalbumin, total and differential WBC (white blood cell) counts, and oxidative stress markers in bronchoalveolar lavage fluid (BALF) were evaluated. In the asthmatic and asthmatic-hyperlipidemic groups, tracheal responsiveness to ovalbumin, total WBC count, numbers of eosinophils, neutrophils, and monocytes were higher than the control group (p<0.001). A left-ward shift in the concentration-response curves to methacholine, an increase in nitrite and malondialdehyde concentrations, and a decrease in total thiol content, superoxide dismutase and catalase activities were also observed in the asthmatic and asthmatic-hyperlipidemic groups compared to control group (p<0.01 to p<0.001). Beyond lipid-lowering effect in the treated hyperlipidemic and asthmatic-hyperlipidemic groups, rosuvastatin treatment decreased tracheal responsiveness to methacholine, reduced total WBC count, the numbers of eosinophils, neutrophils, and monocytes, as well as decreased malondialdehyde concentration, and increased total thiol content, superoxide dismutase and catalase activities in treated asthmatic and asthmatic-hyperlipidemic groups (p<0.05 to p<0.001). The improving effect of rosuvastatin on asthmatic and asthmatic-hyperlipidemic animals was shown due to pleiotropic mechanisms including the effect on airway hyperresponsiveness, lung inflammation, and oxidative stress.

scholarly journals Oxidative Stress Biomarkers as a Predictor of Stage Illness and Clinical Course of Schizophrenia

2021 ◽  
Vol 12 ◽  
Author(s):  
Dariusz Juchnowicz ◽  
Michał Dzikowski ◽  
Joanna Rog ◽  
Napoleon Waszkiewicz ◽  
Anna Zalewska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Regression Model ◽  
Antioxidant Status ◽  
Serum Levels ◽  
Stress Index ◽  
First Episode ◽  
Control Group ◽  
Healthy Individuals ◽  
Related Factors ◽  
Reducing Ability

Pro/antioxidant imbalance has been reported in schizophrenia (SZ). However, the results of studies are inconsistent and usually do not include other factors that are highly affected by oxidative stress (OS).This cross-sectional study aimed to determine the serum levels of OS markers and their potential connection with schizophrenia. The total sample comprised 147: 98 individuals with SZ −47 first-episode (FS) and 49 chronic patients (CS)—and 49 healthy individuals (HC) as a control group. The examination included clinical variables and serum levels of antioxidants and oxidative damage products. The significant changes were observed in concentrations of all examined markers, without any specific direction of the pro/antioxidant balance shift between SZ and HC. In the regression model adjusted for cofounders, catalase: OR = 0.81 (95%CI: 0.74–0.88); glutathione peroxidase: OR = 1.06 (95%CI: 1.02–1.10); total antioxidant capacity: OR = 0.85 (95%CI: 0.75–0.98); oxidative stress index: OR = 1.25 (95%CI: 1.03–1.52); ferric reducing ability of plasma: OR = 0.79 (95%CI: 0.69–0.89); advanced glycation end products: OR = 1.03 (95%CI: 1.01–1.04); and advanced oxidation protein products (AOPP): OR = 1.05 (95%CI: 1.03–1.07) turned out to be significant predictors of schizophrenia. In the multiple stepwise regression model, pro/antioxidant status and their interaction with the duration of illness-related factors affected schizophrenia symptoms: positive symptoms (FRAPxKYN), negative (DITYR, FRAP, CAT), general (KYN), and over-all psychopathology (KYNxNFK). The results confirm differences in serum levels of oxidative biomarkers between SZ patients and healthy individuals. The pro/antioxidant status could be considered a predictor of schizophrenia and the factor affects patients' symptom severity.

scholarly journals Assessment of dynamic thiol/disulfide homeostasis in patients with asthma

2018 ◽  
Vol 42 (3) ◽  
pp. 99-104
Author(s):  
Rukiye Nar ◽  
Aliye Gamze Çalış
Keyword(s):  
Oxidative Stress ◽  
Forced Expiratory Volume ◽  
Control Group ◽  
Reactive Protein ◽  
Total Thiol ◽  
Stress Markers ◽  
Group A ◽  
And Gender ◽  
And Control

Abstract Background: Asthma is a chronic inflammatory lung disease and oxidative stress is an important component in airway inflammation. This study aims to investigate dynamic thiol/disulfide homeostasis in patients with asthma. Methods: A total of 103 subjects, including 56 patients with asthma and 47 healthy controls, of similar age and gender were included in the study. The native thiol, total thiol and disulfide levels and the disulfide-native thiol, disulfide-total thiol and native thiol-total thiol ratios were analyzed and compared between the asthma and control groups using a novel automatized spectrophotometric assay. Results: The levels of native thiol (p<0.001), total thiol (p<0.001) and disulfide (p<0.001) were significantly lower and the C-reactive protein (CRP) levels (p<0.001) were significantly higher in patients with asthma when compared with those in the control group. A negative correlation was detected between CRP levels and native thiol, total thiol and disulfide levels (p<0.05). A significant positive correlation was detected between forced expiratory volume in 1 s (FEV1) and forced vital capacity (FVC) levels and native thiol and total thiol levels (p<0.01). Conclusions: The thiol/disulfide homeostasis parameters may be used as novel oxidative stress markers in asthma but further studies are needed to investigate the role of thiol/disulfide homeostasis in asthma.

scholarly journals Assessment of Serum Enzymatic Antioxidant Levels in Patients with Recurrent Aphthous Stomatitis: A Case Control Study

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Ishita Gupta ◽  
Arvind Shetti ◽  
Vaishali Keluskar ◽  
Anjana Bagewadi
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Serum Levels ◽  
Recurrent Aphthous Stomatitis ◽  
The Body ◽  
Aphthous Stomatitis ◽  
Control Group ◽  
Study Group ◽  
Enzymatic Antioxidant ◽  
Exact Etiology

Background and Aim. Recurrent aphthous stomatitis (RAS) is a common oral mucosal disorder characterized by recurrent, painful oral aphthae. Despite extensive research, the exact etiology of RAS remains elusive. Recently oxidant-antioxidant imbalance of the body has been implicated in the pathogenesis of recurrent aphthous stomatitis. Thus, the aim of the study was to evaluate the enzymatic antioxidant levels in patients with recurrent aphthous stomatitis. Materials and Methods. The serum levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were measured in 30 patients with recurrent aphthous stomatitis and compared to the control group, which included 30 healthy subjects. Student’s t-test was performed for statistical evaluation. Results. The mean levels of superoxide dismutase (130.2 ± 15.94 U/mL) and glutathione peroxidase (3527.93 ± 488.32 U/L) were found to be significantly lower in study group as compared to control group (211.9 ± 20.93 U/mL, 8860.93 ± 1105.31 U/L, resp.) (P=0.000) while level of catalase in study group was significantly higher when compared to control group (10981.00 ± 1018.07 U/mL versus 9764.00 ± 1621.19 U/mL) (P=0.000). Conclusion. Enzymatic antioxidant system is impaired in recurrent aphthous stomatitis patients and seems to play a crucial role in its pathogenesis.

scholarly journals Assessment of Serum Concentrations of Ghrelin, Obestatin, Omentin-1, and Apelin in Children with Type 1 Diabetes

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Agnieszka Polkowska ◽  
Izabela Szczepaniak ◽  
Artur Bossowski
Keyword(s):  
Insulin Resistance ◽  
Type 1 Diabetes ◽  
Disease Duration ◽  
Serum Levels ◽  
The Body ◽  
Control Group ◽  
Study Group ◽  
Newly Diagnosed ◽  
Serum Concentrations

The increasing knowledge on the functions of gastric peptides and adipokines in the body allows the assumption of their major role linking the process of food intake, nutritional status, and body growth, largely through the regulation of glucose metabolism and insulin resistance. The aim of the study was the assessment of serum levels of selected gastric peptides and adipocytokines in children with type 1 diabetes, with respect to the disease duration. The study involved 80 children aged 4–18 years (M/F -37/43). Children with type 1 diabetes (n=46) were compared to the control group (n=34). The study group was divided into 4 subgroups: (I) patients with newly diagnosed type 1 diabetes, after an episode of ketoacidosis (n=10), (II) patients with type 1 diabetes of duration no longer than 5 years (n=9), (III) patients with 5 to 10 years of DT1 (n=20), and (IV) patients with type 1 diabetes of duration longer than 10 years (n=7). The concentrations of gastric peptide and adipocytokines across all subgroups were lower than in the control group. The differences were statistically significant (p<0.0001), which may be of importance in the development of the disease complications.

scholarly journals Oxidative Stress and Antioxidant Status in Schizophrenia Patients

2016 ◽  
Vol 12 (2) ◽  
pp. 40-43
Author(s):  
Fatema Zerin Khan ◽  
Syeda Papia Sultana ◽  
Mohammad SI Mullick ◽  
Nargis Akhter
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Antioxidant Status ◽  
Armed Forces ◽  
Control Group ◽  
Study Group ◽  
Medical College ◽  
Anti Oxidant ◽  
Endogenous Antioxidants ◽  
Medical University

Introduction: Oxidative stress has been assumed to contribute to the pathophysiology of schizophrenia. Increased oxidative stress is the result of either an increased production of free radicals or a depletion of the endogenous antioxidants. Objective: To assess the levels of oxidative stress and antioxidant status in schizophrenia. Materials and Methods: This observational study was carried out in the department of Pharmacology, Bangabandhu Sheikh Mujib Medical University from September 2013 to January 2015. Ninety three schizophrenia patients were enrolled as study group and 30 healthy indivuduals were taken as control group. The peripheral levels of several molecules associated with oxidative stress namely malondialdehyde (MDA), glutathione (GSH) and anti-oxidant status like plasma levels of ascorbic acid (vitamin C) and α-tocopherol (vitamin E) in 93 patients with schizophrenia and 30 healthy participants were assessed. Results: Study found that the schizophrenia group presented substantially higher levels of oxidative stress than the control group, as revealed by elevated quantities of the pro-oxidant MDA (6.3±0.5μmol/L in study group and 2.1±0.5μmol/L in control group), decreased levels of the antioxidants GSH (0.6±0.2mg/gm of Hb in study group and 2.1±0.5mg/gm of Hb in control group), plasma α-tocopherol and ascorbic acid. Results found were highly significant (p=0.001). Conclusion: In schizophrenia there are increased level of oxidative stress and decreased level of the antioxidants. Journal of Armed Forces Medical College Bangladesh Vol.12(2) 2016: 40-43

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Type 2 Diabetes Mellitus: A Retrospective Cohort Study

2020 ◽  
Vol 20 (4) ◽  
pp. 584-590 ◽  
Author(s):  
Shima Fathi ◽  
Shiva Borzouei ◽  
Mohammad Taghi Goodarzi ◽  
Jalal Poorolajal ◽  
Fatemeh Ahmadi-Motamayel
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Type 2 Dm ◽  
Patients With Diabetes ◽  
The Difference ◽  
And Oxidative Stress

Background: Diabetes Mellitus (DM) is a progressive metabolic disorder. Objective: The aim of this study was to investigate the relationship between antioxidant and oxidative stress markers in the saliva of patients with type 2 DM and a healthy control group. Methods: In this study, 20 patients with diabetes and 20 healthy individuals were evaluated. Salivary antioxidants markers consisted of total antioxidant capacity (TAC), uric acid (UA), peroxidase and catalase. Oxidative stress markers included total oxidant status (TOS), malondealdehyde (MDA) and total thiol (SH). Sialochemical analysis was performed with spectrophotometric assay. All the statistical analyses were conducted using STATA software. Results: TAC decreased significantly in patients with diabetes. Although salivary UA and peroxidase were lower in patients with diabetes compared to the control group, the difference was not significant. Salivary catalase in patients with diabetes was significantly lower than that in the control group. MDA and TOS exhibited significantly higher levels in type 2 DM. SH levels were slightly higher in DM. Conclusions: According to the results of the present study, there were some changes in the salivary levels of some antioxidants and oxidative stress markers in patients with type 2 DM and could be measured as an indicator of serum changes..

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