scholarly journals Protective Effects of Lipoxin A4 in Testis Injury following Testicular Torsion and Detorsion in Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Xian-Long Zhou ◽  
Qi-Sheng Yang ◽  
Shao-Zhou Ni ◽  
Xiao-Peng Tu ◽  
Yan Zhao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Single Dose ◽  
Biochemical Analysis ◽  
Testicular Torsion ◽  
Sham Group ◽  
Torsion Group ◽  
Protective Effects ◽  
Lipoxin A4 ◽  
Testicular Morphology

Purpose. To investigate the protective effects of lipoxin A4 (LXA4) in rat testis injury following testicular torsion/detorsion.Methods. A rat testicular torsion model has been established as described. Rats were randomly divided into 6 groups: sham group, torsion group, torsion/detorsion (T/D) group, and T/D plus LXA4-pretreated groups (3 subgroups). Rats in LXA4-pretreated groups received LXA4 injection (0.1, 1.0, and 10 μg/kg body weight in LXA4-pretreated subgroups 1–3, resp.) at a single dose 1 h before detorsion. Biochemical analysis, apoptosis assessment, and morphologic evaluation were carried out after orchiectomies.Results. GPx and SOD levels significantly increased and MDA levels significantly reduced in LXA4-pretreated groups compared to T/D group. LXA4 also reverted IL-2 and TNF-αto basal levels and improved the expression of IL-4 and IL-10 in LXA4-pretreated groups. Moreover, the expression of NF-κB was downregulated in LXA4-pretreated groups. LXA4 treatment also showed an improved testicular morphology and decreased apoptosis in testes.Conclusion. Lipoxin A4 protects rats against testes injury after torsion/detorsion via modulation of cytokines, oxidative stress, and NF-κB activity.

Potential anti-toxic effect of d-ribose-l-cysteine supplement on the reproductive functions of male rats administered cyclophosphamide

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Gabriel O. Oludare ◽  
Gbenga O. Afolayan ◽  
Ganbotei G. Semidara
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Sperm Motility ◽  
Sperm Count ◽  
Male Rats ◽  
Oxidative Enzymes ◽  
Protective Effects ◽  
Testosterone Levels ◽  
Group I ◽  
Antioxidant Defence System

Abstract Objectives This study aimed to access the protective effects of d-ribose-l-cysteine (DRLC) on cyclophosphamide (CPA) induced gonadal toxicity in male rats. Methods Forty-eight male Sprague-Dawley rats were divided into six groups of eight rats each. Group I the control, received distilled water (10 ml/kg), Group II received a single dose of CPA 100 mg/kg body weight intraperitoneally (i.p), Groups III and IV received a single dose of CPA at 100 mg/kg (i.p) and then were treated with DRLC at 200 mg/kg bodyweight (b.w) and 400 mg/kg b.w for 10 days, respectively. Rats in Groups V and VI received DRLC at 200 and 400 mg/kg b.w for 10 days, respectively. DRLC was administered orally. Results Results showed that CPA increased percentage of abnormal sperm cells and reduced body weight, sperm count, sperm motility, follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone levels (p<0.05). CPA also induced oxidative stress as indicated by the increased malondialdehyde (MDA) content and reduced activities of the oxidative enzymes measured (p<0.05). Liver enzymes were elevated while the blood cells production was decreased in the rats administered CPA. DRLC supplementation enhanced the antioxidant defence system as indicated in the reduced MDA levels and increased activities of the antioxidant enzymes when compared with CPA (p<0.05). Bodyweight, sperm count, sperm motility, FSH, and testosterone levels were increased in the CPA + DRLC II group compared with CPA (p<0.05). Conclusions The results of this present study showed that DRLC has a potential protective effect on CPA-induced gonadotoxicity.

Protective Effects of Serotonin and its Derivatives, N-Feruloylserotonin and N-(p-Coumaroyl) Serotonin, Against Cisplatin-Induced Renal Damage in Mice

2019 ◽  
Vol 47 (02) ◽  
pp. 369-383 ◽  
Author(s):  
Chan Hum Park ◽  
Ah Young Lee ◽  
Ji Hyun Kim ◽  
Su Hui Seong ◽  
Eun Ju Cho ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Renal Damage ◽  
Pleiotropic Effect ◽  
Protective Effects ◽  
Related Protein ◽  
Renoprotective Effect ◽  
Serotonin Derivatives ◽  
Mitogen Activated Protein ◽  
Apoptosis Related Protein

This study examined whether serotonin and two of its derivatives, [Formula: see text]-feruloylserotonin and [Formula: see text]-([Formula: see text]-coumaroyl) serotonin, have a renoprotective effect in a mouse model of cisplatin-induced acute renal failure. Cisplatin (20[Formula: see text]mg/kg body weight) was administered by intraperitoneal injection to male BALB/c mice that had received oral serotonin, [Formula: see text]-feruloylserotonin or [Formula: see text]-([Formula: see text]-coumaroyl) serotonin (7.5[Formula: see text]mg/kg body weight per day) during the preceding 2 days. At 3 days after the cisplatin injection, serum and renal biochemical factors, oxidative stress, inflammation and apoptosis-related protein expression were evaluated, and histological examinations were performed. Cisplatin caused reduction in body weight and an increase in kidney weight; however, [Formula: see text]-([Formula: see text]-coumaroyl) serotonin and [Formula: see text]-feruloylserotonin attenuated these effects. Moreover, the serotonin derivatives significantly decreased serum urea nitrogen and creatinine levels. They also significantly reduced the level of reactive oxygen species and upregulated the expression of glutathione peroxidase in the kidney. Furthermore, the serotonin derivatives improved the abnormal expression of mitogen-activated protein kinases activation-dependent inflammation- and apoptosis-related protein and caused less renal damage. These results provide important evidence that [Formula: see text]-([Formula: see text]-coumaroyl) serotonin and [Formula: see text]-feruloylserotonin exert a pleiotropic effect on several parameters related to oxidative stress, inflammation and apoptosis. The derivatives also have a renoprotective effect in cisplatin-treated mice; however, this effect is higher with [Formula: see text]-([Formula: see text]-coumaroyl) serotonin.

scholarly journals Anti-Inflammatory and Antioxidative Effects of Sumatriptan Against Doxorubicin-Induced Cardiotoxicity in Rat

2021 ◽  
Author(s):  
Mohammad Sheibani ◽  
Hedyeh Faghir-Ghanesefat ◽  
Yaser Azizi ◽  
Tahmineh Mokhtari ◽  
Hasan Yousefi‐Manesh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Mortality Rate ◽  
Cardiac Tissue ◽  
The Body ◽  
Male Rats ◽  
Protective Effects ◽  
Cardiac Damage ◽  
Necrosis Factor Alpha ◽  
Antioxidative Effects

The clinical use of doxorubicin as a potent chemotherapeutic agent is limited due to its dose-dependent cardiotoxicity. Oxidative stress and inflammatory pathways have a pivotal role in doxorubicin-induced cardiotoxicity. Sumatriptan, a 5-hydroxytryptamine (5-HT)1B/1D agonist that is mainly used to relieve migraine pain, has suggested exerting protective effects in numerous pathological conditions through antiinflammatory properties. The aim of the present study was to investigate the effects of sumatriptan on doxorubicin-induced cardiotoxicity and the contribution of anti-inflammation and antioxidative responses. Cardiotoxicity was induced by the administration of doxorubicin three times a week (2.5 mg/kg i.p) for two consecutive weeks on male rats. The animals were divided into four groups, including Control, Sumatriptan (0.1 mg/kg) received group, doxorubicin received group, and Doxorubicin+Sumatriptan (0.1 mg/kg) received group. Sumatriptan was administered 30 min before every injection of doxorubicin. On the last day of the second week, the body weight, mortality rate, electrocardiogram (ECG) and histopathological changes, cardiac inotropic study, and biochemical factors were evaluated. The loss of body weight, mortality rate, ECG parameters, reduction of papillary muscle contractility force as well as histopathological scores following administration of doxorubicin indicated severe cardiac damage. However, treatment with sumatriptan inhibited the functional and structural impairment induced by doxorubicin. In addition, sumatriptan could significantly reduce cardiac tissue levels of malondialdehyde (MDA) and tumor necrosis factor-alpha (TNF-α), which were increased in the doxorubicin-treated rats. This study illustrated the protective effects of sumatriptan on decreasing doxorubicin-induced cardiac toxicity and mortality rate in part through inhibition of inflammatory and oxidative stress pathways.

scholarly journals Neuroprotective Effects of Thymol, a Dietary Monoterpene Against Dopaminergic Neurodegeneration in Rotenone-Induced Rat Model of Parkinson’s Disease

2019 ◽  
Vol 20 (7) ◽  
pp. 1538 ◽  
Author(s):  
Hayate Javed ◽  
Sheikh Azimullah ◽  
MF Meeran ◽  
Suraiya Ansari ◽  
Shreesh Ojha
Keyword(s):  
Oxidative Stress ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Body Weight ◽  
Therapeutic Potential ◽  
Nigrostriatal System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protective Effects ◽  
Neuroprotective Effects ◽  
Dopaminergic Neurodegeneration

Parkinson’s disease (PD), a multifactorial movement disorder that involves progressive degeneration of the nigrostriatal system affecting the movement ability of the patient. Oxidative stress and neuroinflammation both are shown to be involved in the etiopathogenesis of PD. The aim of this study was to evaluate the therapeutic potential of thymol, a dietary monoterpene phenol in rotenone (ROT)-induced neurodegeneration in rats that precisely mimics PD in humans. Male Wistar rats were injected ROT at a dose of 2.5 mg/kg body weight for 4 weeks, to induce PD. Thymol was co-administered for 4 weeks at a dose of 50 mg/kg body weight, 30 min prior to ROT injection. The markers of dopaminergic neurodegeneration, oxidative stress and inflammation were estimated using biochemical assays, enzyme-linked immunosorbent assay, western blotting and immunocytochemistry. ROT challenge increased the oxidative stress markers, inflammatory enzymes and cytokines as well as caused significant damage to nigrostriatal dopaminergic system of the brain. Thymol treatment in ROT challenged rats appears to significantly attenuate dopaminergic neuronal loss, oxidative stress and inflammation. The present study showed protective effects of thymol in ROT-induced neurotoxicity and neurodegeneration mediated by preservation of endogenous antioxidant defense networks and attenuation of inflammatory mediators including cytokines and enzymes.

Hepatoprotective Effects of Algerian Crataegus oxyacantha Leaves

2019 ◽  
Vol 10 (1) ◽  
pp. 70-75
Author(s):  
Amira Mecheri ◽  
Wassila Benabderrahmane ◽  
Amel Amrani ◽  
Nassima Boubekri ◽  
Fadila Benayache ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Female Rats ◽  
Protective Effects ◽  
Edible Plant ◽  
Traditional Use ◽  
Butanol Extract ◽  
Hepatic Diseases ◽  
Pharmaceutical Industries ◽  
Hepatoprotective Effects

Background: Hawthorn (C. oxyacantha), a common edible plant, is widely used for the preparation of a different foodstuff and is also used in traditional medicine to treat heart problems and gastrointestinal ailments. Recently, a few patents of Crataegus preparation for protective effects (prevention of cardiovascular and hepatic diseases) have been developed. </P><P> Objective: The current study aimed to explore the antioxidant and hepatoprotective effects of nbutanol extract of Crataegus oxyacantha leaves in acute liver damage induced by Doxorubicin (DOX). </P><P> Methods: Crataegus oxyacantha (100 mg/kg body weight) or vitamin E as a standard antioxidant (100 mg/kg body weight) were administered orally to female rats for 10 days, in the presence or absence of hepatotoxicity induced by a single intraperitoneal (i.p.) injection of DOX (15 mg/kg on the 8th day). On day 11, blood and liver samples were analyzed for biomarker levels and histopathological changes. Liver homogenates were used for determination of oxidative stress parameters that include Malondialdehyde (MDA), Glutathione (GSH) level and Glutathione Peroxidase (GPx) activity. </P><P> Results: Treatment with n-butanol extract of C. oxyacantha leaves significantly improved the altered liver enzyme activities and oxidative stress markers. The histopathological observations confirm the results of biochemical parameters. </P><P> Conclusion: The obtained results support the traditional use of C. oxyacantha to cure gastrointestinal ailments and highlighted its possible use in the food and pharmaceutical industries as a source of natural antioxidant.

Crocin attenuates cisplatin-induced liver injury in the mice

2013 ◽  
Vol 33 (8) ◽  
pp. 855-862 ◽  
Author(s):  
Y Sun ◽  
J Yang ◽  
L-Z Wang ◽  
L-R Sun ◽  
Q Dong
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Caspase 3 ◽  
Antitumor Agents ◽  
Mitogen Activated Protein Kinase ◽  
Protective Effects ◽  
Once Daily ◽  
Liver Histopathology ◽  
Mitogen Activated Protein ◽  
Serum Aspartate Aminotransferase

Cisplatin (CDDP) is one of the most frequently used antitumor agents, but its application is significantly limited by its hepatotoxicity. In the present study, we investigated the effects of crocin against CDDP-induced oxidative stress and apoptosis in the liver of Kunming mice. Crocin was administered to the mice once daily for 7 consecutive days at the doses of 6.25 and 12.5 mg/kg body weight orally. On day 1, a single intraperitoneal injection of CDDP was given at the dose of 10 mg/kg body weight. Crocin treatment significantly improved CDDP-induced hepatic damage as indicated by serum aspartate aminotransferase and alanine aminotransferase levels. Crocin relieved CDDP-induced oxidative stress by reducing malondialdehyde level and recovering the levels of glutathione and antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase. In addition, liver histopathology indicated that crocin alleviated CDDP-induced focal necrosis. Immunohistochemical staining and Western blot analysis showed that crocin significantly decreased the levels of phospho-p38 mitogen-activated protein kinase (MAPK), tumor protein 53 (p53), and cleaved caspase-3. Taken together, our data suggest that crocin provides protective effects against CDDP-induced hepatoxicity by attenuating oxidative stress and inhibiting the activation of p38 MAPK, p53, and caspase-3.

scholarly journals Hydroalcoholic Extract of Iranian Caper Leaves Protects Hepatic Toxicity by Suppressing Oxidative Stress in Mice

2019 ◽  
Author(s):  
Heibatullah Kalantari ◽  
Anis Alijani ◽  
Parvin Kheradmand ◽  
Maedeh Goodarzian ◽  
Leila Zeidooni
Keyword(s):  
Oxidative Stress ◽  
Sham Group ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Oxygen Species ◽  
Protective Effects ◽  
Aromatic Plant ◽  
Hydroalcoholic Extract ◽  
Capparis Spinosa ◽  
Hepatic Histopathology

Capparis spinosa L. (caper) is an aromatic plant, commonly used in the Mediterranean diet, possessing numerous antioxidant compounds, such as phenols, rutin, tocopherols, carotenoids, and vitamin C in its leaves. Thus, the present study investigated the effects of Iranian caper leaves extract on oxidative stress caused by CCl4 in the mice’s liver. This study was conducted on 42 male mice in seven groups. The control group, the sham group, the CCl4 group, the Iranian caper leaves extract 100, 200, and 400 mg/kg + CCl4 groups. Then, Biochemicals, oxidative stress, and hepatic histopathology parameters were evaluated. The co-administration of Iranian caper leaves extract, and CCl4 significantly decreased the levels of aspartate aminotransferase, alanine aminotransferase, and reactive oxygen species, malondialdehyde (P<0.001) and significantly increased the levels of glutathione and catalase in comparison with the group treated with CCl4 alone (P<0.01).Furthermore, Iranian caper leaves extract improved histopathological changes such as the the inflammation and necrosis of hepatocytes. Iranian caper leaves extract has protective effects on hepatotoxicity induced by CCl4, mainly through suppressing oxidative stress.

scholarly journals Aqueous extract from Cinnamomum zeylanicum (Lauraceae) stem-bark ameliorates gentamicin-induced nephrotoxicity in rats by modulating oxidative stress and inflammatory markers

2021 ◽  
Author(s):  
ATSAMO Albert ◽  
LONTSIE Auscar SONGMENE ◽  
Mireille Flaure METCHI DONFACK ◽  
Omer Bébé NGOUATEU KENFACK ◽  
Télesphore Benoît NGUELEFACK ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Aqueous Extract ◽  
Relative Weight ◽  
Stem Bark ◽  
Negative Control ◽  
Control Group ◽  
Protective Effects ◽  
Cinnamomum Zeylanicum ◽  
Reference Drug

Abstract Nephropathies and especially nephrotoxicity has become one of serious cause of life threatening condition, because of intensive exposure to xenobiotic either by environmental pollution or drug abuse. The present study was undertaken to assess the protective effects of Cinnamomum zeylanicum stem-bark aqueous extract (AECZ) on gentamicin-induced nephrotoxicity. AECZ was prepared by maceration in water and tested orally at the doses of 200 and 400 mg/kg/day to prevent gentamicin induced nephropathies in male Wistar rats. Gentamicin (100 mg/kg/day) was administered for 14 consecutive days by intraperitoneal route, concomitantly with AECZ or silymarin (50 mg/kg/day) used as reference drug. Animal body weight was monitored during the treatment. After the last treatment of the 14 th day, a 24h urine was collected and animals were sacrificed. Blood was collected for the evaluation of hematological and renal function biomarkers. The homogenate of one kidney was used to assess oxidative stress markers and pro-inflammatory cytokine, while the other one was fixed in formaldehyde for histopathological studies. Gentamicin decreased body weight, serum total proteins and calcemia, but increased kidneys relative weight, serum creatinine, urea and uric acid. Moreover, the levels of reduced glutathione, catalase and superoxide dismutase activities were decreased, while an increase in malondialdehyde, proinflammatory cytokines (TNFα, IL-1β, IL-6) and nitrites were observed in negative control group as compared to normal control. Histological analysis of the kidney revealed the presence of tubular necrosis, glomerular degeneration and macrophage infiltration in gentamicin treated group. All these impairment parameters were prevented by AECZ and silymarin treatments.AECZ has a protective effect against gentamicin-induced nephrotoxicity. The antioxidant and anti-inflammatory potentials of this extract may highly contribute to its nephroprotective activity.

scholarly journals EFFECT OF VITAMIN E ACETATE SUPPLEMENTATION ON THYROID HORMONE-SENSITIVE ORGANS FOLLOWING EXOGENOUS L- THYROXINE TREATMENT

2018 ◽  
Vol 11 (4) ◽  
pp. 123
Author(s):  
Sabyasachi Sinha ◽  
Arijit Chakraborty ◽  
Chiranjit Mondal ◽  
Amar K Chandra
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Vitamin E ◽  
Thyroid Hormone ◽  
Health Management ◽  
Control Treatment ◽  
Protective Effects ◽  
Vitamin E Acetate ◽  
Liver And Kidney ◽  
Hormone Sensitive

 Objective: L-thyroxine is used for control and prevention of many thyroidal diseases, though it may cause damages in thyroid hormone-sensitive organs, namely, liver and kidney. Reports on the protective effects of any antioxidants in L-thyroxine induced oxidative stress are scanty. Thus, L-thyroxine induced oxidative stress and its prevention by Vitamin E supplementation have been studied in the present investigation.Methods: Adult, male Wister rats were divided into four groups of six animals each, and L-thyroxine (T4) (0.3 mg/kg body weight) was administered intraperitoneally in the treated group. Similarly, L-thyroxine (T4), at the above-mentioned dose, and Vitamin E acetate (100 mg/kg of body weight/ day orally) coadministered simultaneously (T4+VE) in the next group. Third group was administered only with Vitamin E, and the remaining group kept as control. Treatment continued regularly for 15 and 30 days. Animals were sacrificed after completion of treatment. Lipid peroxidation (LPO) level, superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) activities were assayed in liver and kidney along with their histology. Obtained results were interpreted statistically against their respective control groups.Results: Body weight was significantly decreased, and relative kidney weight was increased after L-thyroxine administration as compared to control (p<0.05). LPO level, SOD and catalase activities were significantly enhanced in L-thyroxine treated groups, whereas GPx activity was decreased. However, LPO level and the activities of those enzymes along with body weight and organ weights were almost restored their normal in L-thyroxine and Vitamin E coadministered group treated for 15 days and 30 days, respectively.Conclusion: Exogenously administered L-thyroxine causes oxidative stress in liver and kidney that in turn generates reactive oxygen species resulting cell damages. Vitamin E acetate supplementation reduces these adverse effects on liver and kidney and thus acts as a beneficial health management agent.

scholarly journals Cucurbituril Ameliorates Liver Damage Induced by Microcystis aeruginosa in a Mouse Model

2021 ◽  
Vol 9 ◽  
Author(s):  
Na'il Saleh ◽  
Saad Al-Jassabi ◽  
Ali H. Eid ◽  
Werner M. Nau
Keyword(s):  
Body Weight ◽  
Liver Function ◽  
Microcystis Aeruginosa ◽  
Liver Damage ◽  
Biochemical Analysis ◽  
Protective Effects ◽  
Glutathione S Transferase ◽  
Cyclic Heptapeptide ◽  
Liver Enlargement ◽  
Dose Tolerance

Microcystis aeruginosa is a cyanobacterium that produces a variety of cyclic heptapeptide toxins in freshwater. The protective effects of the macromolecular container cucurbit[7]uril (CB7) were evaluated using mouse models of cyanotoxin-induced liver damage. Biochemical analysis of liver function was performed to gauge the extent of liver damage after exposure to cyanobacterial crude extract [CCE; LD50 = 35 mg/kg body weight; intraperitoneal (i.p.)] in the absence or presence of CB7 (35 mg/kg body weight, i.p.). CCE injection resulted in liver enlargement, potentiated the activities of alanine aminotransferase (ALT) and glutathione S-transferase (GST), increased lipid peroxidation (LPO), and reduced protein phosphatase 1 (PP1) activity. CCE-induced liver enlargement, ALT and GST activities, and LPO were significantly reduced when CB7 was coadministered. Moreover, the CCE-induced decline of PP1 activity was also ameliorated in the presence of CB7. Treatment with CB7 alone did not affect liver function, which exhibited a dose tolerance of 100 mg/kg body wt. Overall, our results illustrated that the addition of CB7 significantly reduced CCE-induced hepatotoxicity (P &lt; 0.05).

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