Clerodendrum volubile Ethanol Leaf Extract: A Potential Antidote to Doxorubicin-Induced Cardiotoxicity in Rats

Doxorubicin is widely applied in hematological and solid tumor treatment but limited by its off-target cardiotoxicity. Thus, cardioprotective potential and mechanism(s) of CVE in DOX-induced cardiotoxicity were investigated using cardiac and oxidative stress markers and histopathological endpoints. 50–400 mg/kg/day CVE in 5% DMSO in distilled water were investigated in Wistar rats intraperitoneally injected with 2.5 mg/kg DOX on alternate days for 14 days, using serum troponin I and LDH, complete lipid profile, cardiac tissue oxidative stress marker assays, and histopathological examination of DOX-treated cardiac tissue. Preliminary qualitative and quantitative assays of CVE’s secondary metabolites were also conducted. Phytochemical analyses revealed the presence of flavonoids (34.79 ± 0.37 mg/100 mg dry extract), alkaloids (36.73 ± 0.27 mg/100 mg dry extract), reducing sugars (07.78 ± 0.09 mg/100 mg dry extract), and cardiac glycosides (24.55 ± 0.12 mg/100 mg dry extract). 50–400 mg/kg/day CVE significantly attenuated increases in the serum LDH and troponin I levels. Similarly, the CVE dose unrelatedly decreased serum TG and VLDL-c levels without significant alterations in the serum TC, HDL-c, and LDL-c levels. Also, CVE profoundly attenuated alterations in the cardiac tissue oxidative stress markers’ activities while improving DOX-associated cardiac histological lesions that were possibly mediated via free radical scavenging and/or antioxidant mechanisms. Overall, CVE may play a significant therapeutic role in the management of DOX-induced cardiotoxicity in humans.

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African Vegetables (Clerodendrum volibile Leaf and Irvingia gabonensis Seed Extracts) Effectively Mitigate Trastuzumab-Induced Cardiotoxicity in Wistar Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/9535426 ◽

2020 ◽

Vol 2020 ◽

pp. 1-15

Author(s):

Olufunke Olorundare ◽

Adejuwon Adeneye ◽

Akinyele Akinsola ◽

Sunday Soyemi ◽

Alban Mgbehoma ◽

...

Keyword(s):

Oxidative Stress ◽

Wistar Rats ◽

Cardiac Tissue ◽

Histopathological Examination ◽

Radical Scavenging ◽

Metastatic Breast ◽

Heart Tissue ◽

Oxidative Stress Markers ◽

Her2 Positive ◽

Stress Markers

Trastuzumab (TZM) is a humanized monoclonal antibody that has been approved for the clinical management of HER2-positive metastatic breast and gastric cancers but its use is limited by its cumulative dose and off-target cardiotoxicity. Unfortunately, till date, there is no approved antidote to this off-target toxicity. Therefore, an acute study was designed at investigating the protective potential and mechanism(s) of CVE and IGE in TZM-induced cardiotoxicity utilizing cardiac enzyme and oxidative stress markers and histopathological endpoints. 400 mg/kg/day CVE and IGE dissolved in 5% DMSO in sterile water were investigated in Wistar rats injected with 2.25 mg/kg/day/i.p. route of TZM for 7 days, using serum cTnI and LDH, complete lipid profile, cardiac tissue oxidative stress markers assays, and histopathological examination of TZM-intoxicated heart tissue. Results showed that 400 mg/kg/day CVE and IGE profoundly attenuated increases in the serum cTnI and LDH levels but caused no significant alterations in the serum lipids and weight gain pattern in the treated rats. CVE and IGE profoundly attenuated alterations in the cardiac tissue oxidative stress markers’ activities while improving TZM-associated cardiac histological lesions. These results suggest that CVE and IGE could be mediating its cardioprotection via antioxidant, free radical scavenging, and antithrombotic mechanisms, thus, highlighting the therapeutic potentials of CVE and IGE in the management of TZM-mediated cardiotoxicity.

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Evaluating the protective potency of Acacia hydaspica R. Parker on histological and biochemical changes induced by Cisplatin in the cardiac tissue of rats

BMC Complementary and Alternative Medicine ◽

10.1186/s12906-019-2575-8 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 5

Author(s):

Tayyaba Afsar ◽

Suhail Razak ◽

Ali Almajwal ◽

Maria Shabbir ◽

Muhammad Rashid Khan

Keyword(s):

Oxidative Stress ◽

Creatine Kinase ◽

Antioxidant Enzymes ◽

Cardiac Function ◽

Cardiac Tissue ◽

Troponin I ◽

Antioxidant Properties ◽

Oxidative Stress Markers ◽

Oxidative Stress Biomarkers ◽

Stress Markers

Abstract Background Increase oxidative trauma is the main cause behind Cisplatin (CP) induced cardiotoxicity which restricts its clinical application as anti-neoplastic prescription. Acacia hydaspica is a natural shrub with diverse bioactivities. Acacia hydaspica ethyl acetate extract (AHE) ameliorated drug-induced cardiotoxicity in animals with anti-oxidative mechanisms. Current study aimed to evaluate the protective potential of A. hydaspica against cisplatin-induced myocardial injury. Methods Rats were indiscriminately distributed into six groups (n = 6). Group 1: control; Groups 2: Injected with CP (7.5 mg/kg bw, i.p, single dose) on day 16; Group 3: Treated for 21 days with AHE (400 mg/kg b.w, oral); Group 4: Received CP injection on day 16 and treated with AHE for 5 days post injection; Group 5: Received AHE (400 mg/kg b.w/day, p.o.) for 21 days and CP (7.5 mg/kg b.w., i.p.) on day 16; Group 6: Treated with silymarin (100 mg/kg b.w., p.o.) after 1 day interval for 21 days and CP injection (7.5 mg/kg b.w., i.p.) on day 16. On 22nd day, the animals were sacrificed and their heart tissues were removed. Cisplatin induced cardiac toxicity and the influence of AHE were evaluated by examination of serum cardiac function markers, cardiac tissue antioxidant enzymes, oxidative stress markers and histology. Results CP inoculation considerably altered cardiac function biomarkers in serum and diminished the antioxidant enzymes levels, while increased oxidative stress biomarkers in cardiac tissues AHE treatment attenuated CP-induced deteriorations in creatine kinase (CK), Creatine kinase isoenzymes MB (CK-MB), cardiac Troponin I (cTNI) and lactate dehydrogenase (LDH) levels and ameliorated cardiac oxidative stress markers as evidenced by decreasing lipid peroxidation, H2O2 and NO content along with augmentation in phase I and phase II antioxidant enzymes. Additionally, CP inoculation also induced morphological alterations which were ameliorated by AHE. In pretreatment group more significant protection was observed compared to post-treatment group indicating preventive potential of AHE. The protective potency of AHE was comparable to silymarin. Conclusion Results demonstrate that AHE attenuated CP induce cardiotoxicity. The polyphenolic metabolites and antioxidant properties of AHE might be responsible for its protective influence.

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Cardiac troponins and oxidative stress markers in non-pregnant, pregnant and preeclampsia women

Bangladesh Medical Research Council Bulletin ◽

10.3329/bmrcb.v36i1.4806 ◽

2010 ◽

Vol 36 (1) ◽

pp. 4-9 ◽

Cited By ~ 11

Author(s):

Palanisamy Pasupathi ◽

Uma Manivannan ◽

Perisamy Manivannan ◽

Mathiyalagan Deepa

Keyword(s):

Oxidative Stress ◽

Troponin I ◽

Troponin T ◽

Enzymatic Antioxidants ◽

Pregnancy Induced Hypertension ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Induced Hypertension ◽

And Oxidative Stress ◽

In Pregnancy

Free radicals play an important role in the pathogenesis of tissue damage in many clinical disorders, including atherosclerosis. This study was to investigate lipids and oxidative stress markers among women with 50 healthy non-pregnant compare with 50 healthy pregnant and 50 pregnancy-induced hypertensive subjects and correlate with cardiac troponin I (cTnI) and troponin T (cTnT). The level of plasma thiobarbituric acid reactive substances (TBARS), cTnI and cTnT levels significantly increase in pregnancy-induced hypertension compare with other groups. The level of lipids significantly altered in pregnancy-induced hypertension. Conversely, the activities of both enzymatic and non-enzymatic antioxidants were significantly decreased in pregnancy-induced hypertension compared to non-pregnant and healthy pregnant. Our data suggest that there is an imbalance between lipoperoxidation and antioxidants levels during pregnancy and preeclampsia. Serum cTnI and cTnT are elevated in women with pregnancy-induced hypertension indicating some degree of cardiac myofibrillar damage and cardiac dysfunction.Online: 11 July 2010DOI: http://dx.doi.org/10.3329/bmrcb.v36i1.4806Bangladesh Med Res Counc Bull 2010; 36: 4-9

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Aberrations in Oxidative Stress Markers in Amyotrophic Lateral Sclerosis: A Systematic Review and Meta-Analysis

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/1712323 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 12

Author(s):

Zihao Wang ◽

Zhile Bai ◽

Xiaoyan Qin ◽

Yong Cheng

Keyword(s):

Oxidative Stress ◽

Meta Analysis ◽

Density Lipoprotein ◽

Protein Product ◽

Oxidative Stress Marker ◽

Oxidative Stress Markers ◽

Stress Marker ◽

Stress Markers ◽

Als Patients ◽

Lateral Sclerosis

Oxidative stress has been reported to be involved in the onset and development of amyotrophic lateral sclerosis (ALS). Data from clinical studies have highlighted increased peripheral blood oxidative stress markers in patients with ALS, but results are inconsistent. Therefore, we quantitatively pooled data on levels of blood oxidative stress markers in ALS patients from the literature using a meta-analytic technique. A systematic search was performed on PubMed and Web of Science, and we included studies analyzing blood oxidative stress marker levels in patients with ALS and normal controls. We included 41 studies with 4,588 ALS patients and 6,344 control subjects, and 15 oxidative stress marker levels were subjected to random-effects meta-analysis. The results demonstrated that malondialdehyde (Hedges’ g, 1.168; 95% CI, 0.812 to 1.523; P<0.001), 8-hydroxyguanosine (Hedges’ g, 2.194; 95% CI, 0.554 to 3.835; P=0.009), and Advanced Oxidation Protein Product (Hedges’ g, 0.555; 95% CI, 0.317 to 0.792; P<0.001) levels were significantly increased in patients with ALS when compared with control subjects. Uric acid (Hedges’ g, -0.798; 95% CI, -1.117 to -0.479; P<0.001) and glutathione (Hedges’ g, -1.636; 95% CI, -3.020 to -0.252; P=0.02) levels were significantly reduced in ALS patients. In contrast, blood Cu, superoxide dismutase, glutathione peroxidase, ceruloplasmin, triglycerides, total cholesterol, low-density lipoprotein, high-density lipoprotein, coenzyme-Q10, and transferrin levels were not significantly different between cases and controls. Taken together, our results showed significantly increased blood levels of 8-hydroxyguanosine, malondialdehyde, and Advanced Oxidation Protein Product and decreased glutathione and uric acid levels in the peripheral blood of ALS patients. This meta-analysis helps to clarify the oxidative stress marker profile in ALS patients, supporting the hypothesis that oxidative stress is a central component underpinning ALS pathogenesis.

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Physiochemical Studies of Sodium Chloride on Mungbean (Vigna radiataL. Wilczek) and Its Possible Recovery with Spermine and Gibberellic Acid

The Scientific World JOURNAL ◽

10.1155/2015/858016 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 12

Author(s):

Srijita Ghosh ◽

Sanglap Mitra ◽

Atreyee Paul

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Gibberellic Acid ◽

Chlorophyll Content ◽

Biomass Production ◽

Oxidative Stress Marker ◽

Antioxidant Enzyme Activities ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Low Concentrations

The physiological and biochemical responses to increasing NaCl concentrations, along with low concentrations of gibberellic acid or spermine, either alone or in their combination, were studied in mungbean seedlings. In the test seedlings, the root-shoot elongation, biomass production, and the chlorophyll content were significantly decreased with increasing NaCl concentrations. Salt toxicity severely affected activities of different antioxidant enzymes and oxidative stress markers. Activities of antioxidant enzymes, superoxide dismutase (SOD), and catalase (CAT) increased significantly over water control. Similarly, oxidative stress markers such as proline, malondialdehyde (MDA), and hydrogen peroxide (H2O2) contents also increased as a result of progressive increase in salt stress. Combined application of NaCl along with low concentrations of either gibberellic acid (5 µM) or spermine (50 µM) in the test seedlings showed significant alterations, that is, drastic increase in seedling elongation, increased biomass production, increased chlorophyll content, and significant lowering in all the antioxidant enzyme activities as well as oxidative stress marker contents in comparison to salt treated test seedlings, leading to better growth and metabolism. Our study shows that low concentrations of either gibberellic acid or spermine will be able to overcome the toxic effects of NaCl stress in mungbean seedlings.

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Active recovery training does not affect the antioxidant response to soccer games in elite female players

British Journal Of Nutrition ◽

10.1017/s0007114510002394 ◽

2010 ◽

Vol 104 (10) ◽

pp. 1492-1499 ◽

Cited By ~ 16

Author(s):

Helena Andersson ◽

Anette Karlsen ◽

Rune Blomhoff ◽

Truls Raastad ◽

Fawzi Kadi

Keyword(s):

Oxidative Stress ◽

Antioxidant Response ◽

Oxidative Stress Marker ◽

Oxidative Stress Markers ◽

Active Recovery ◽

Dietary Antioxidants ◽

Antioxidant Defence ◽

Stress Markers ◽

Dietary Antioxidant ◽

Endogenous Antioxidant

Changes in plasma endogenous and dietary antioxidants and oxidative stress markers were studied following two 90 min elite female soccer games separated by 72 h of either active or passive recovery. The active recovery group (n 8) trained for 1 h at 22 and 46 h after the first game (low-intensity cycling and resistance training), while the passive group rested (n 8). Blood samples were taken before the games; immediately after the games; 21, 45 and 69 h after the first game; and immediately after the second game. The oxidative stress markers and antioxidants were not affected by active recovery. The oxidative stress marker GSSG increased by the same extent after both the games, while the lipid peroxidation marker diacron-reactive oxygen metabolite remained unchanged. The endogenous antioxidants total glutathione and uric acid and ferric reducing/antioxidant power increased immediately after both the games with the same amplitude, while increases in cysteine, cysteine–glycine and total thiols reached significant levels only after the second game. The changes in dietary antioxidants after the first game were either rapid and persistent (tocopherols and ascorbic acid (AA) increased; polyphenols decreased) or delayed (carotenoids). This resulted in high pre-second game levels of tocopherols, AA and carotenoids. Polyphenols returned to baseline at 69 h, and were not affected by the second game. In conclusion, the soccer-associated dietary antioxidant defence, but not the endogenous antioxidant defence, is persistent. Similar acute oxidative stress and endogenous antioxidant responses and dissimilar dietary antioxidant reactions occur during two repeated female soccer games. Finally, the complex antioxidant response to soccer is not affected by active recovery training.

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Protective Effect of Hydroethanolic Extracts of Solanum scabrum and Cola verticillata Against Cyclophosphamide Induced Toxicity in Female Rats

Journal of Food Research ◽

10.5539/jfr.v3n3p18 ◽

2014 ◽

Vol 3 (3) ◽

pp. 18 ◽

Cited By ~ 1

Author(s):

Mbong Angie M-A ◽

Djiokeng Paka G. ◽

Ntentie F. R. ◽

Dimodi H. ◽

Ngondi J. L. ◽

...

Keyword(s):

Oxidative Stress ◽

Intraperitoneal Administration ◽

Female Rats ◽

Protective Effects ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Significant Toxicity ◽

Cyclophosphamide Treatment ◽

Hydroethanolic Extract ◽

Antioxidant Mechanisms

The aim of this study was to evaluate the protective effects of hydroethanolic extracts of S. scabrum and C. verticillata against cyclophosphamide induced toxicity. In this light, female albino wistar rats were treated by intraperitoneal administration of 100 mg/kg BW of cyclophosphamide or distilled water every other day for 7 days associated with oral gavage using hydroethanolic extract of C. verticillata/S. scabrum at a dose of 200 or 400 mg/kg BW or not every day for the same 7 days. On the 8th day, blood and organs (liver, heart and kidney) were collected for analyses of toxicity-related and oxidative stress markers. Cyclophosphamide treatment induced significant toxicity as shown by liver enzymes, urea and creatinine levels. The administration of extracts helped reduce the levels of these markers. The antioxidant effect of these extracts also helped or not to ameliorate oxidative stress markers (MDA, NO, hydroperoxides, catalase, thiols, GPx) depending on the extract and on the dose administered. These results suggest that administration of hydroethanolic extracts of S. scabrum and C. verticillata can help prevent or reduce toxicity that is brought about by treatment with cyclophosphamide due to their ability to upregulate antioxidant mechanisms.

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The Effects of Resveratrol on Oxidative Stress Markers: A Systematic Review and Meta-Analysis of Randomized Clinical Trials

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530319666191116112950 ◽

2020 ◽

Vol 20 (5) ◽

pp. 718-727 ◽

Cited By ~ 2

Author(s):

Mahsa Omidian ◽

Mina Abdolahi ◽

Elnaz Daneshzad ◽

Mohsen Sedighiyan ◽

Mohadeseh Aghasi ◽

...

Keyword(s):

Oxidative Stress ◽

Systematic Review ◽

Clinical Trials ◽

Randomized Clinical Trials ◽

Meta Analysis ◽

Serum Levels ◽

Oxidative Stress Marker ◽

Cochrane Library ◽

Oxidative Stress Markers ◽

Stress Markers

Objective: Recent trial studies have found that resveratrol supplementation beneficially reduces oxidative stress marker, but, there is no definitive consensus on this context. The present systematic review and meta-analysis aimed to investigate the effect of resveratrol supplementation on oxidative stress parameters. Methods: We searched databases of Pubmed, Scopus and Cochrane Library up to December 2018 with no language restriction. Studies were reviewed according to preferred reporting items for systematic reviews and meta-analyses (PRISMA) and Cochrane handbook. To compare the effects of resveratrol with placebo, weighted mean difference (WMD) with 95% confidence intervals (CI) were pooled based on the random-effects model. Results: Among sixteen clinical trials, we found that resveratrol supplementation increased GPx serum levels significantly (WMD: 18.61; 95% CI: 8.70 to 28.52; P<0.001) but had no significant effect on SOD concentrations (WMD: 1.01; 95% CI: -0.72 to 2.74; P= 0.25), MDA serum levels (WMD: -1.43; 95% CI: -3.46 to 0.61; P = 0.17) and TAC (WMD: -0.09; 95% CI: -0.29 to 0.11; P = 0.36) compared to placebo. Finally, we observed that resveratrol supplementation may not have a clinically significant effect on oxidative stress. Conclusion: However, the number of human trials is limited in this context, and further large prospective clinical trials are needed to confirm the effect of resveratrol supplement on oxidative stress markers.

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Multi-organ Toxicity Attenuation by Cerium Oxide and Yttrium Oxide Nanoparticles: Comparing the Beneficial Effects on Tissues Oxidative Damage Induced by Sub-acute Exposure to Diazinon

Pharmaceutical Nanotechnology ◽

10.2174/2211738508666200808135226 ◽

2020 ◽

Vol 8 (3) ◽

pp. 225-238

Author(s):

Mona Navaei-Nigjeh ◽

Marzieh Daniali ◽

Mahban Rahimifard ◽

Mohammad R. Khaksar

Keyword(s):

Oxidative Stress ◽

Cerium Oxide ◽

Metal Oxide Nanoparticles ◽

Radical Scavenging ◽

Oxide Nanoparticles ◽

Oxidative Stress Markers ◽

Beneficial Effects ◽

Stress Markers ◽

Kidney Liver ◽

The Brain

Background: Excessive use of diazinon, as an organophosphate pesticide (OP), contributes to cytotoxic and pathologic cellular damage and, in particular, oxidative stress. However, metal-oxide nanoparticles (NPs), such as cerium oxide (CeO2) and yttrium oxide (Y2O3), with the property of free radical scavenging demonstrated beneficial effects in the alleviation of oxidative stress biomarkers. Objective: The aims of this study include evaluating beneficial effects of CeO2 NPs, Y2O3 NPs, and their combination against diazinon-induced oxidative stress in different tissues of brain, heart, lung, kidney, liver, and spleen. Methods: Eight randomized groups of 6 adult male Wistar rats were formed. Each group of rats administered a different combination of diazinon, CeO2 and Y2O3 NPs daily and levels of oxidative stress markers, such as reactive oxygen species (ROS), lipid peroxidation (LPO), total thiol molecules (TTM) and total anti-oxidant power (TAP) and catalase enzyme, were measured after 2 weeks of the treatment. Results: Measurements of the mentioned markers in the brain, heart, lung, kidney, liver, and spleen showed that the administration of NPs could significantly alleviate the oxidative stress induced by diazinon. However, the findings of this study illustrated that the combination of both CeO2 and Y2O3 NPs led to a better reduction in oxidative stress markers. Conclusion: Sub-acute exposure of diazinon in rats led to increased levels of oxidative stress markers in pivotal tissues such as the brain, heart, lung, kidney, liver, and spleen. CeO2 and Y2O3 NPs neutralize the oxidative stress to compensate diazinon-induced tissue damages. Lay Summary: Organophosphate pesticides (OPs), which are mainly used for pest control, are responsible for the entry of pesticides into the human food cycle. Organophosphate such as diazinon increases the molecular biomarkers of oxidative stress inside the cells of vital tissues such as the heart, liver, lungs, etc. Metal oxide nanoparticles (NPs) such as cerium oxide (CeO2) and yitrium oxide (Y2O3) can have free radical scavenging potential under oxidative stress and through various mechanisms. Although these nanoparticles reduce oxidative stress, it should be borne in the design of the study that additional doses of these substances reverse the beneficial effects.

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Adiposity and Fat Distribution in relation to Inflammation and Oxidative Stress in a Relatively Lean Population of Chinese Women

Disease Markers ◽

10.1155/2013/437076 ◽

2013 ◽

Vol 34 (4) ◽

pp. 279-293 ◽

Cited By ~ 5

Author(s):

Sheng-Hui Wu ◽

Xiao-Ou Shu ◽

Wong-Ho Chow ◽

Yong-Bing Xiang ◽

Xianglan Zhang ◽

...

Keyword(s):

Oxidative Stress ◽

Chinese Women ◽

Plasma Concentrations ◽

Anthropometric Measurements ◽

Oxidative Stress Marker ◽

Oxidative Stress Markers ◽

Anthropometric Measures ◽

Stress Markers ◽

Markers Of Inflammation ◽

And Oxidative Stress

Objectives: This study evaluated associations of various anthropometric measures of adiposity with a panel of inflammatory and oxidative stress markers in a relatively lean population of Chinese women.Methods: This analysis included 1,005 Chinese women aged 40–70 years. Plasma concentrations of inflammatory and oxidative stress markers were measured. Anthropometric measurements were taken by trained interviewers.Results: Body mass index (BMI), waist circumference (WC), and waist-to-height ratio (WHtR) were all positively and linearly associated with the inflammatory markers, CRP, TNF-α, soluble TNF-receptor 1 (sTNF-R1), and IL-6. A significant positive association of these measures of adiposity with the oxidative stress marker F2-IsoP-M, a metabolite of F2-IsoPs, but with not F2-IsoPs was found. Differences in biomarkers between extreme quartiles of anthropometric measurements varied widely, ranging from 9.7% for sTNF-R1 to 162.0% for CRP. For each specific biomarker, various anthropometric measurements exhibited similar ability to explain variations in the biomarker, with the biggest partial r2(11%) observed for CRP.Conclusions: This study suggests that both general adiposity (measured by BMI) and central adiposity (measured by WC and WHtR) are positively and similarly associated with various markers of inflammation and oxidative stress in relatively lean Chinese women. The metabolite F2-IsoP-M of F2-IsoPs may be a better marker ofin vivooxidative stress than its parent compounds.

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