scholarly journals Assessment of Laser Biostimulation in Induction of Ovulation

2018 ◽  
Vol 6 (7) ◽  
pp. 1193-1198
Author(s):  
Mohamed A. El-Nourya ◽  
Mahmoud B. El-Begawyb ◽  
Mona E. Morsyc ◽  
Ehab M. Al Sherbiny
Keyword(s):  
Clomiphene Citrate ◽  
Study Groups ◽  
Control Group ◽  
Albino Rats ◽  
Serum Estradiol ◽  
Serum Progesterone ◽  
Induction Of Ovulation ◽  
Significant Difference ◽  
Conventional Medical Therapy ◽  
Wistar Albino Rats

AIM: This study aimed to evaluate a new modality of low power laser biostimulation in rat ovaries, in comparison with the conventional medical therapy by clomiphene citrate that depends on up-regulation of the hypothalamic-pituitary-ovarian axis to induce ovulation.STUDY DESIGN: A Prospective experimental study carried out from January 2014 to February 2016.SETTING: University-based photobiology laboratory.MATERIALS AND METHODS: Seventy-two (72) Female-Wistar Albino rats were used in this study, divided into three groups: 17 rats used as a control group, 19 rats received clomiphene citrate (Clomid group), 36 rats exposed to diode laser 660 nm wavelength (laser group).RESULTS: Biochemical assessment of serum Estradiol and serum Progesterone was done in the three study groups. Serum Estradiol & Progesterone levels were statistically significantly higher in clomiphene and laser treated groups than non-interventional controls, while no significant difference between clomiphene and laser groups as regard of both hormones.CONCLUSION: This study shows that ovarian laser biostimulation is a new encouraging method for induction of ovulation, at least in animals. This had been proven biochemically by a significant increase in serum Estradiol and serum Progesterone.

Clomiphene Citrate or Letrozole for Ovulation Induction in Women with Polycystic Ovarian Syndrome: A Prospective Randomized Trial

2010 ◽  
Vol 2 (2) ◽  
pp. 169-173
Author(s):  
Randhir Puri ◽  
Ahmed Badawy ◽  
Ibrahim Abdel Aal ◽  
Mohamed Abulatta
Keyword(s):  
Randomized Trial ◽  
Ovulation Induction ◽  
Polycystic Ovary ◽  
Endometrial Thickness ◽  
Clomiphene Citrate ◽  
University Teaching ◽  
Prospective Randomized Trial ◽  
Serum Estradiol ◽  
Serum Progesterone ◽  
Significant Difference

Objective To compare the effects of letrozole (5 mg) and clomiphene citrate (100 mg) for ovulation induction in women with polycystic ovary syndrome (PCOS). Design Prospective randomized trial. Setting University teaching hospital and private practice setting. Patients The study comprised a total of 438 infertile women (1063 cycles) with PCOS. Intervention Patients were randomized to treatment with 5 mg of letrozole daily (218 patients, 545 cycles) or 100 mg of clomiphene citrate daily (220 patients, 518 cycles) for 5 days starting on day 3 of menses. Timed intercourse was advised 24 to 36 hours after hCG injection. Main Outcome Measure Number of follicles, serum estradiol, serum progesterone, endometrial thickness, and pregnancy and miscarriage rates. Results The mean age, parity, and duration of infertility in both groups were similar. The total number of follicles was statistically significantly greater in the clomiphene citrate group (6.8 _ 0.3 vs 4.4 _ 0.4). Endometrial thickness at the time of hCG administration was statistically significantly greater in the CC group (9.2 _ 0.7 mm vs 8.1 _ 0.2 mm). The duration to reach a dominant follicle was statistically significantly longer in the letrozole group (12.1_1.3 vs 8.8_2.9 days). Ovulation occurred in 365 out of 540 cycles (67.5%) in letrozole group and 371 out of 523 cycles (70.9%) without a statistically significant difference. Levels of serum estradiol and progesterone were statistically significantly higher in the clomiphene citrate group. The pregnancy rate per cycle was 15.1% in the letrozole group and 17.9% in the clomiphene citrate group without statistically difference between the groups. Conclusion The results of this study did not show any advantage to the use of letrozole over clomiphene citrate as a first-line treatment for induction of ovulation in women with PCOS (Fertil Steril_2009;92:849-52. _2009 by American Society for Reproductive Medicine).

scholarly journals Tetrapleura tetraptera Extract Inhibited Luteinizing Hormone and Estrogen Secretion in Clomiphene Citrate Treated Female Wistar Albino Rats

2019 ◽  
pp. 1-6
Author(s):  
E. O. Agbai ◽  
C. O. Nwanegwo ◽  
C. J. Njoku ◽  
P. C. Onyebuagu ◽  
J. Ekezie ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Clomiphene Citrate ◽  
Control Group ◽  
Albino Rats ◽  
Pituitary Cells ◽  
Progesterone Secretion ◽  
Tetrapleura Tetraptera ◽  
Group A ◽  
Wistar Albino Rats ◽  
Group D

Tetrapleura tetraptera stem bark has been reported to cause inhibition of luteinizing hormone release in cultured rat pituitary cells. Hence, we investigated the effects of Tetraptera tetrapleura pod extract on follicle stimulating hormone, luteinizing hormone, cortisol, progesterone and estrogen. Thirty non-pregnant female wistar albino rats were divided into group A - D. Group A rats were used as Control. Group B rats were administered 1 mg/kg/day of clomiphene citrate orally. Group C rats were administered 200 mg/kg of extract only, whereas group D rats were administered 1 mg/kg /day of Clomiphene citrate plus 200 mg/kg of extract. At the end of 14 days experiment, group A, B and D were found in proestrus phase and group C in diestrus phase. FSH and cortisol levels remained unchanged. Group C and D rats produced significant reduction (P < 0.05) in LH and estrogen levels in prolonged proestrus and normal diestrus respectively. Progesterone level was significantly high (P < 0.05) in the group C rats. The reduced LH level could be due to the anti-estrogenic effect of extract during proestrus when LH secretion is expected to surge. But co-administration resulted in high progesterone secretion, suggesting extract may have influenced progesterone secretion in group D rats simultaneously administered with clomiphene citrate and extract. The above findings indicated that Tetrapleura tetraptera pod extracts inhibited luteinizing hormone and estrogen even when co-administered with clomiphene citrate.

scholarly journals Hepatotoxicity and biochemical evaluation of a novel breakfast food produced from the blend of fonio (Digitaria iburua Stapf) and pigeon pea (Cajanus cajan (L.) Millspaugh) in albino rats

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Grace O. Babarinde ◽  
Akos A. Ebun ◽  
Peter I. Adegbola
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Density Lipoprotein ◽  
Experimental Period ◽  
Pigeon Pea ◽  
Control Group ◽  
Albino Rats ◽  
Significant Difference ◽  
Breakfast Food ◽  
Wistar Albino Rats

Abstract Background The production of novel foods from underutilized nutritious tropical crops is increasing; however, the toxicological studies are sparse. In this study, the safety of the breakfast food produced from the blend of fonio and pigeon pea was examined by evaluating the biochemical and toxicological parameters of the breakfast food using Wistar albino rats. Twenty male Wistar albino rats subdivided into 4 groups of 5 were separately fed with four food samples (100% fonio, 80:20 fonio:pigeon pea, standard rat feed (control) and a commercial brand of corn flake) for 28 days after which the animals were sacrificed and blood samples collected for analyses. Body weight, blood glucose, biochemical, and hematological parameters were examined during the experimental period. Results The feeding of rats with the four diets did not cause mortality and adverse toxicity during the experimental period. Steady body weight gain was observed in fonio-pigeon pea fed rats, and slight changes were obtained in percentage blood glucose. There was no significant difference in the levels of white blood cells, lymphocytes, mean corpuscular volume, platelet, high-density lipoprotein, catalase, SOD, and malondialdehyde of the group fed with fonio-pigeon pea diet as compared with the control group. Furthermore, significant (p < 0.05) lower levels of density lipoprotein, triacylglyceride, and cholesterol were observed in the fonio-pigeon pea-fed rats than the control group. The liver function marker of fonio-pigeon pea-fed-rat and the control showed no significant difference. Conclusion The study showed no severe toxicity in the test animals, although a mild loss of liver parenchyma and variation in size and shape of nuclei were observed in the group fed with the fonio-pigeon pea diet.

scholarly journals Effect of Moderate Intake of Ethanol on Lipid Profile of Wistar-Albino Rats Fed High Fat Diet

2020 ◽  
Vol 2 (3) ◽  
Author(s):  
Kathiravan Vayanaperumal ◽  
Ravindran Rajan
Keyword(s):  
Lipid Profile ◽  
Blood Cholesterol ◽  
Ethanol Administration ◽  
Control Group ◽  
Albino Rats ◽  
High Fat ◽  
Group I ◽  
Significant Difference ◽  
Wistar Albino Rats ◽  
The Impact

Many evidences suggest that light to moderate alcohol drinking may provide some cardiovascular benefits. The present study examined the impact of how moderate ethanol consumption provides a protective effect against high blood cholesterol. Wistar-Albino rats were divided into a control group (I) and test groups (II, III, IV). The test groups (II, III, IV) were administered with a predefined dosage of 10%, 20% and 30% ethanol respectively following twenty days of high fat routine however, control animals were managed with normal saline for the next fifteen days. Significant difference in lipid profile parameters witnessed before and after ethanol administration between the control and test groups. Findings from the study suggests that 10% moderate ethanol is adequately least enough to reduce the risk of cardiovascular disease by improving the blood cholesterol in Wistar-Albino rats.

scholarly journals An Effective Phytoconstituent Aconitine: A Realistic Approach for the Treatment of Trigeminal Neuralgia

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Dilek Çankal ◽  
Esra Küpeli Akkol ◽  
Yeliz Kılınç ◽  
Mert İlhan ◽  
Raffaele Capasso
Keyword(s):  
Spinal Cord ◽  
Trigeminal Neuralgia ◽  
Motor Coordination ◽  
Infraorbital Nerve ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Significant Difference ◽  
Wistar Albino Rats ◽  
Dose Dependent

Trigeminal neuralgia pain remains a challenge to treat. Natural compounds may be promising options for relieving pain. This study was aimed at investigating the effects of aconitine in a rat model of trigeminal neuralgia pain. Infraorbital nerve chronic constriction injury was performed in adult Wistar Albino rats. After the neuropathic pain developed, the rats were assigned to one of the treatment groups: carbamazepine 40 or 80 mg/kg; aconitine 0.25, 0.50, or 0.75 mg/kg; or saline injection (control group). Behavioral testing with von Frey filaments and the rotarod test were carried out before the surgical procedure and on the 24th to 29th postoperative days. Following the completion of tests, ipsilateral and contralateral spinal cords were harvested for Western blot analyses to assess NR-1 protein expression. ANOVA followed by Mann-Whitney U test was performed for the statistical analyses. P values of <0.05 were considered significant. Aconitine significantly reduced mechanical sensitivity in a dose-dependent manner. A significant reduction in motor coordination was noted for the higher doses of aconitine which was similar with the 40 and 80 mg/kg doses of carbamazepine. NR-1 expression was reduced in the ipsilateral spinal cord, whereas no significant difference was noted between the groups in the expression of NR-1 in the contralateral spinal cord. Aconitine had a significant pain relieving effect, which was similar to carbamazepine, in a dose-dependent manner. Aconitine may be an alternative pharmacological agent for the control of trigeminal neuralgia pain.

scholarly journals An Experimental Study of Ethanolic Extract of Myristica fragrans in Morphine Dependence

2016 ◽  
Vol 15 (2) ◽  
pp. 224-229 ◽  
Author(s):  
Imran Zaheer ◽  
Syed Ziaur Rahman ◽  
Rahat Ali Khan ◽  
Mehtab Parveen
Keyword(s):  
Medical Science ◽  
Ethanolic Extract ◽  
Study Groups ◽  
Morphine Dependence ◽  
Control Group ◽  
Albino Rats ◽  
Abstinence Syndrome ◽  
Myristica Fragrans ◽  
Wistar Albino Rats

Objective: To evaluate the role of ethanolic extract of Myristica fragrans in morphine dependence. Methods: Wistar albino rats were made moderate and severe grade morphine dependence by administering morphine sulphate in dose of 10 mg /kg (i.p.), twice daily for 4 days and by increasing doses of 10-100 mg/kg (i.p.), twice daily for 7 days, respectively. The signs of spontaneous abstinence syndrome were recorded 12 hours in both studies after the last dose of morphine for 30 minutes and quantified by ‘counted’ and ‘checked’ signs. Ethanolic extract of Myristica fragrans (EEMF) was administered p.o. in different regimen: (a) EEMF 200 mg/kg along with morphine twice daily for 4 days and 7 days in moderately and severely induced morphine dependence group, respectively. (b) EEMF 400 mg/kg (p.o.), single dose10 hours after the last dose of morphine in both moderately and severely induced morphine dependence rats.Result: Oral administration of EEMF in both study groups caused significant reduction in the scores of counted and checked signs of morphine abstinence syndrome as compared to active morphine control group. The reduction was significantly more in regimen ‘a’ as compared to regimen ‘b’.Conclusion: Ethanolic extract of Myristica fragrans seed significantly reduced the mean scores of various’counted signs’ and ‘checked signs’ of morphine withdrawal syndrome and might give a solution as a substitute therapy in morphine de-addiction.Bangladesh Journal of Medical Science Vol.15(2) 2016 p.224-229

scholarly journals The effects of adding Gonadotropin-releasing Hormone antagonist on cycle characteristics and pregnancy rate in stimulated Intrauterine Insemination (IUI) cycle

2020 ◽  
Vol 11 (3) ◽  
pp. 3053-3060
Author(s):  
Rawaa saad Hassan Abunaila ◽  
Lubna Amer Al-anbari ◽  
Muayad Sraibet Abbood
Keyword(s):  
Pregnancy Rate ◽  
Assisted Reproductive Technologies ◽  
Intrauterine Insemination ◽  
Clomiphene Citrate ◽  
Study Groups ◽  
Reproductive Technologies ◽  
Control Group ◽  
Human Menopausal Gonadotropin ◽  
Study Group ◽  
Significant Difference

A prospective randomised (comparative) study was conducted in the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies in AL-Nahrain University, Baghdad – Iraq, from the 1st of October 2018 till 1st of September 2019 involving seventy women of infertile couples with the same inclusion and exclusion criteria. They were randomly allocated into two groupsreceiving minimal ovarian stimulation protocol (Clomiphene Citrate and Human Menopausal Gonadotropin) with IUI adding flexible GnRH antagonist protocol (Cetrorelix) to the study group. IUI was done 40-44 hours after ovulation trigger. There were no significant statistical variances among control and study groups in demographic characteristics concerning: age, BMI, type, duration and cause of infertility. There was no important variance in a mean day of trigger among control and study groups, 12.43 ±1.56 versus 13.11 ±1.49, at the same order (p = 0.064). The Mean number of dominant follicles was considerably greater in study groups than that of the group control, 2.40±1.03 versus 1.89±0.83, respectively (p = 0.025).Mean serum Estradiol of the study group was significantly higher than that of the control group, 478.68 ±423.61 versus 273.12 ±254.57, respectively (p = 0.016). The proportion of women with LH> 10 were significantly less frequent in the study group in comparison with the control group, 22.9 % versus 68.6 %, at the same order (p < 0.001). There was no important variance in mean serum Progesterone among control and study groups. There was no significant difference in the characteristic of the dominant follicle, ruptured versus not ruptured, (p = 0.124) on the day of IUI. The pregnancy rate was higher in the study group when compared with the control group; however, the difference in pregnancy rate doesn’t reach statistical import (p = 0.145)

scholarly journals Partial Effect of Resveratrol on Wound Healing: Study on Wistar Albino Rats

2021 ◽  
Author(s):  
Tayfun Bilgiç ◽  
Nazlı Caf ◽  
Ayşe Nilhan Atsü
Keyword(s):  
Wound Healing ◽  
Blood Perfusion ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
New Therapeutic Approaches ◽  
Significant Difference ◽  
Carcinogenic Effects ◽  
Wistar Albino Rats ◽  
And Control

Abstract Purpose: There are multiple and specific phases during wound healing. Resveratrol (RSV) is a polyphenol compound found naturally in fruits. Resveratrol's antioxidant, anti-aging, cardioprotective, anti-inflammatory, and anti-carcinogenic effects have been shown. This study aims to determine the effects of RSV on perfusion, neovascularization, inflammation, and granulation stages of wound healing in addition to its antioxidant capacity over GPX and SOD.Methods: 16 female rats were divided into two groups as the RSV and control groups. Six excisions were made in each rat. Three excisions in each rat were sutured and the other 3 were left as open wounds. Topical RSV was applied to the wounds of the rats in the RSV group. No treatment was given to the control group. Blood perfusion of the wounds in both groups were measured using the PeriScan PIM 3 System Laser Doppler Blood Perfusion Imager. Punch biopsies were taken for histopathological examinations and for the evaluation of SOD, GPX, and MDA.Results: GPX level of the closed wounds belonging to the RSV group was higher compared to the control group. There was no statistically significant difference in SOD and MDA levels between the groups. Neovascularization was registered higher in the open wounds of RSV group. Conclusion: Within parameters, resveratrol has been shown to be effective in wound healing in various ways. The results of this study may shed light on new therapeutic approaches that can be used in the reversal of oxidative stress in wound-causing diseases.

Effect of Propolis-Supplemented Diet on Body Composition and Endocrine Function of Adipose Tissue

2020 ◽  
Vol 19 (2) ◽  
pp. 217-221
Author(s):  
Maria Jesús Lisbona-González ◽  
Candela Reyes-Botella ◽  
Esther Muñoz-Soto ◽  
Maria Victoria Olmedo-Gaya, ◽  
Jorge Moreno-Fernandez ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Composition ◽  
Adipose Tissue ◽  
Endocrine Function ◽  
Control Group ◽  
Albino Rats ◽  
Standard Diet ◽  
Reduced Body ◽  
Esterified Fatty Acids ◽  
Wistar Albino Rats

Adipose tissue is an endocrine organ and has central role in interaction with other organs or tissues while propolis can induce lipolysis. Therefore, the aim of this study is to provide detailed information about adipose tissue homeostasis modifications and body composition during propolis supplement consumption. Twenty male Wistar albino rats (8 weeks) were divided into two groups of 10 animals each and fed for 90 days with two different types of diets: standard for the control group (diet C) and standard diet + 2% propolis (diet P). Thyroid hormones did not show differences, while ghrelin and adiponectin decreased in the group that was fed propolis. Insulin, leptin, and non-esterified fatty acids also increased along with reduced body weight and fat, in addition to increased lean mass when propolis was in the diet. We conclude that propolis could decrease ghrelin and adiponectin but increase non-esterified fatty acids and insulin secretion, which improves body composition.

Success Rate of Obturation of Root Canals by Different Techniques in Primary Molars: A Comparative Clinical Study

2020 ◽  
Vol 2 (1) ◽  
pp. 36-44
Author(s):  
Satyawan G. Damle ◽  
Ritika Bansal ◽  
Dhanashree D. Sakhare
Keyword(s):  
Success Rate ◽  
Study Groups ◽  
Primary Molars ◽  
Control Group ◽  
Second Primary ◽  
Chi Square ◽  
Exact Test ◽  
Root Canals ◽  
Significant Difference ◽  
Comparative Clinical Study

Objective: To compare the success rate of different obturation procedures in primary mandibular second molars clinically and also by digital radiovisiography. Methods: A total of 40 children aged between 4-8 years with deeply carious mandibular second primary molars indicated for single session pulpectomy were selected. Canals were obturated with Metapex. The 3 study groups (Endodontic plugger, Handheld lentulospiral, Navi Tip syringe) were compared with the control group (reamer) both clinically and radiovisiographically. The data collected were statistically analyzed using Pearson’s Chi-square and Fisher’s exact test. Results: The use of Navi tip syringe led to the least number of voids followed by Endodontic plugger and Reamer and the highest number of voids was reported with Lentulospiral. Navitip presented maximum number of optimally filled cases followed by Endodontic plugger and Lentulospiral and least number of optimally filled cases with reamer. However, there was no statistically significant difference (p>0.05) in any of the groups with clinical (pain and tenderness to percussion) and radiographic parameters (presence or absence of voids and length of obturation). Conclusion: Within the limitations of the present study, though the clinical outcome was statistically insignificant, Navitip syringe exhibited encouraging results and is a promising option for obturation in primary teeth.

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