scholarly journals Clinical effect of Changweishu on gastrointestinal dysfunction in patients with sepsis

2020 ◽  
Vol 48 (8) ◽  
pp. 030006052091957
Author(s):  
Fen-Qiao Chen ◽  
Wen-Zhong Xu ◽  
Hai-Yun Gao ◽  
Li-Juan Wu ◽  
He Zhang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Treatment Group ◽  
Western Medicine ◽  
Clinical Effect ◽  
Apache Ii ◽  
Inflammatory Factors ◽  
Apache Ii Score ◽  
Control Group ◽  
Gastrointestinal Dysfunction ◽  
Tnf Α

Objective To investigate Changweishu’s clinical effect on gastrointestinal dysfunction in patients with sepsis. Methods Fifty patients with gastrointestinal dysfunction and sepsis were randomly divided into treatment and control groups. The control group patients received routine Western medicine treatments (meropenem, noradrenaline, glutamine glue, Bifidobacterium lactis triple-strain tablet), and the treatment group patients received routine Western medicine treatment combined with Changweishu. Treatments in both groups lasted 7 days. Changes in APACHE II score, gastrointestinal dysfunction score, serum levels of diamine oxidase (DAO), D-lactic acid, inflammatory factors (tumor necrosis factor (TNF)-α, interleukin (IL)-6, and high-mobility group box 1 (HMGB-1)), and the incidence of multiple organ dysfunction syndrome (MODS) and mortality were observed. Results After treatment, APACHE II score, gastrointestinal dysfunction score, and DAO, D-lactic acid, TNF-α, IL-6, and HMGB-1 levels decreased significantly in both groups, but the decrease was more significant in the treatment group than in the control group. The incidence of MODS and mortality were significantly lower in the treatment group than in the control group. Conclusion The addition of Changweishu to routine Western treatments can improve gastrointestinal function in patients with sepsis and gastrointestinal dysfunction, as well as decreasing the incidence of MODS and mortality and improving patient prognosis.

scholarly journals The Efficacy of Moxibustion on the Serum Levels of CXCL1 and β-EP in Patients with Rheumatoid Arthritis

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Siyu Tao ◽  
Xue Wang ◽  
Chenxi Liao ◽  
Yan Xiong ◽  
Jie Tang ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Drug Therapy ◽  
Treatment Group ◽  
Serum Levels ◽  
Inflammatory Factors ◽  
Control Group ◽  
Tnf Α ◽  
Before And After ◽  
Chemokine Ligand ◽  
Pain Symptoms

Objective. This study aims to evaluate the efficacy of moxibustion on joint swelling and pain and the levels of C-X-C motif chemokine ligand 1 (CXCL1), β-endorphin (β-EP) in serum of rheumatoid arthritis (RA) patients and to investigate the anti-inflammatory and analgesic mechanism of moxibustion on improving RA. Methods. Sixty-eight patients with RA were randomly and equally classified into the control and treatment groups. The control group was treated with routine drug therapy, while the treatment group received routine drug therapy and moxibustion. Both groups were treated for eight weeks. The symptoms and laboratory indicators of RA patients were compared in the two groups before and after intervention. Results. Sixty-one patients completed the study: four patients dropped out from the treatment group and three from the control group. Trial endpoints were change (∆) in symptoms, measured by Ritchie’s articular index (RAI), swollen joint count (SJC), and laboratory indicators, measured by the level of CXCL1, β-EP, tumor necrosis factor-a (TNF-α), and interleukin-1β (IL-1β). ∆RAI, ∆SJC, ∆CXCL1, ∆β-EP, ∆TNF-α, and ∆IL-1β in the treatment group were superior to the control group (13.50 [14.50] versus 6.00 [13.00] in ∆RAI, 4.00 [3.00] versus 2.00 [4.00] in ∆SJC, 0.04 ± 0.79 ng/mL versus -0.01 ± 0.86 ng/mL in ∆CXCL1, -2.43 [5.52] pg/mg versus -0.04 [4.09] pg/mg in ∆β-EP, 3.45 [5.90] pg/mL versus 1.55 [8.29] pg/mL in ∆TNF-α, and 6.15 ± 8.65 pg/mL versus 1.28 ± 8.51 pg/mL in ∆IL-1β; all P  < 0.05). Conclusion. Moxibustion can improve the joint swelling and pain symptoms in patients with RA, which may be related to the fact that moxibustion can reduce the release of inflammatory factors in patients with RA and downregulate the level of CXCL1 and increase the level of β-EP at the same time. This trial is registered with ChiCTR-IOR-17012282.

scholarly journals Reducing postsurgical exudate in breast cancer patients by using San Huang decoction to ameliorate inflammatory status: a prospective clinical trial

2018 ◽  
Vol 25 (6) ◽  
Author(s):  
Z. Y. Zhu ◽  
J. X. Xue ◽  
L. X. Yu ◽  
W. H. Bian ◽  
Y. F. Zhang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Clinical Trial ◽  
Treatment Group ◽  
Cancer Patients ◽  
Symptom Score ◽  
Inflammatory Factors ◽  
Control Group ◽  
Breast Cancer Patients ◽  
Drainage Fluid ◽  
Tnf Α

Background Reducing inflammatory factors in wound exudate is a promising treatment approach for healing wounds in postsurgical breast cancer patients. Traditional Chinese Medicine (tcm) treatments have been shown to be beneficial and safe for optimal regulation of oxidative stress during the postoperative period. In the present clinical trial, we evaluated the effectiveness of a promising Chinese herbal formula, San Huang decoction [shd (Radix astragali, Radix et rhizoma rhei, and Rhizoma curcuma longa, 3:1:1; supplemental Table 1)], on wound inflammatory response after mastectomy.Methods The study randomized 30 patients with breast cancer who fulfilled the inclusion and exclusion criteria to either a treatment (n = 15) or a control group (n = 15). Patients in the treatment group received liquid shd, taken twice daily with or without food. Treatment was given for 1 day before surgery and for 7 days postoperatively. Participants in the control group received a placebo on the same schedule as the treatment group. Outcomes measured in every subject included clinical tcm and wound inflammation symptom scores, daily and total amounts of drainage fluid, and levels of inflammatory factors in the exudate [tumour necrosis factor α (tnf-α), interleukins 6 (il-6), 8 (il-8), and 2R (il-2R), human C-reactive protein (crp)] at 2 hours and on days 1, 3, and 7 postoperatively.Results The total amount of drainage fluid over 7 days was significantly lower in the treatment group (572.20 } 93.95 mL) than in the control group (700.40 } 107.38 mL). The tcm symptom score was also lower in treatment group (day 7: 1.87 } 0.83 vs. 4.80 } 3.61, p = 0.049), as was the inflammatory symptom score (day 7: 0.67 } 0.72 vs. 3.67 } 2.50, p = 0.001). Levels of tnf-α, il-6, il-8, il-2R, and crp in drainage fluid were significantly lower with shd treatment.Conclusions Perioperative treatment with shd effectively lessened postoperative exudate and ameliorated inflammatory symptoms in patients who underwent surgery for breast cancer..

scholarly journals Effect of Moxibustion on β-EP and Dyn Levels of Pain-Related Indicators in Patients with Rheumatoid Arthritis

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Yingni Wang ◽  
Siyu Tao ◽  
Zeyun Yu ◽  
Yun Luo ◽  
Yuan Li ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Treatment Group ◽  
Clinical Symptoms ◽  
Inflammatory Factors ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Tnf Α ◽  
Significant Difference ◽  
Routine Inspection ◽  
Anti Inflammatory

Background. Rheumatoid arthritis (RA) is a systemic immunodeficiency disease characterized by persistent synovial inflammation, pannus formation, and bone and cartilage destruction, resulting in joint malformations and function decline. Objective. The purpose of this study is to evaluate the effect of moxibustion on clinical symptoms and levels of pain-related indicators beta-endorphin (β-EP) and dynorphin (Dyn) in patients with RA and to explore the potential anti-inflammatory and analgesic mechanisms of moxibustion in RA treatment. Methods. A total of 64 patients with RA who met the inclusion criteria were randomly and equally classified into the control and treatment groups. The control group received conventional treatment (oral methotrexate, folate, or leflunomide prescribed for a long time). The treatment group was treated with moxibustion at ST36 (Zusanli), BL23 (Shenshu), and Ashi points with respect to the control group. Patients’ clinical symptoms and routine inspection indexes (rheumatoid factor [RF], erythrocyte sedimentation rate [ESR], and C-reactive protein [CRP]) were recorded before and after treatment. Serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), β-EP, and Dyn were determined by enzyme-linked immunosorbent assay (ELISA). The software SPSS24.0 was used for statistical analysis. Results. (1) Compared with the pretreatment result, both of the two groups’ clinical symptoms and routine inspection indexes (RF, ESR, and CRP) improved ( P  < 0.05), and the improvement of clinical symptoms in the treatment group outperformed that in the control group ( P  < 0.05). (2) TNF-α and IL-1β levels decreased significantly in the treatment group after treatment ( P  < 0.01), while no significant difference was observed in the control group ( P  > 0.05). (3) β-EP and Dyn levels in the treatment group were significantly increased after treatment ( P  < 0.01, P  < 0.01), but the control group showed no significant difference ( P  > 0.05, P  > 0.05). It is worth mentioning that the serum TNF-α, IL-1β, β-EP, and Dyn levels between the two groups were significantly different after 8 weeks of treatment ( P  < 0.05). (4) Differences in the serum β-EP and Dyn levels in the patients of the treatment group were correlated with TNF-α and IL-1β levels after treatment, and the correlation was mainly negative (r < 0). Conclusion. Moxibustion can improve joint pain in patients with RA using conventional western medicine. One of the mechanisms may affect the serum β-EP and Dyn levels by downregulating the inflammatory factors to play an anti-inflammatory and analgesic role.

scholarly journals Η ανοσολογική απόκριση ολικού ανθρώπινου αίματος σε πρόκληση με στοιχεία του τοιχώματος μυκήτων

2014 ◽  
Author(s):  
Σταύρος Αλοΐζος
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Systemic Inflammatory Response Syndrome ◽  
Inflammatory Response ◽  
Ex Vivo ◽  
Systemic Inflammatory Response ◽  
Apache Ii ◽  
Apache Ii Score ◽  
Gram Negative ◽  
Tnf Α ◽  
Sigma Chemical

Η σήψη αντιπροσωπεύει ένα σοβαρό πρόβλημα υγείας, που σχετίζεταιμε υψηλή θνητότητα και θνησιμότητα, παρατεταμένη παραμονή για νοσηλείαστο Νοσοκομείο, και αυξημένα άμεσα και έμμεσα κόστη. Ειδικά στην ΜΕΘ, ησήψη είναι το πρώτο αίτιο θανάτου. Ακολούθως με τα αποτελέσματα τηςμελέτης Sepsis Occurrence in Acutely Ill Patients (SOAP), 35% των βαρέωςπασχόντων ασθενών αναπτύσσουν σήψη σε κάποιο σημείο της νοσηλείαςτους στην ΜΕΘ, με θανατηφόρες επιπλοκές στο 27% από αυτές, έναποσοστό που πλησιάζει το 50% σε ασθενείς με σηπτικό σοκ. Τα Grampositive[gram (+)] βακτήρια έχουν αναδειχθεί ως το συχνότερο αίτιο λοίμωξηςτων βαρέως πασχόντων ασθενών, ακολουθούμενα από τα gram-negative[gram (-)] βακτήρια και από τους μύκητες, οι οποίοι εμπλέκονται σε περίπου18% των περιπτώσεων.Η ΧΝΑ συχνά επιπλέκεται από λοιμώξεις, ειδικά όταν φτάνει στο τελικότης στάδιο που απαιτεί αιμοδιάλυση. Η θνητότητα των ασθενών με τελικούσταδίου ΧΝΑ είναι περίπου 20%, με τις καρδιαγγειακές παθήσεις και τιςλοιμώξεις να ενοχοποιούνται για το 70% των θανάτων. Η αυξημένη επίπτωσητης σήψης στους ασθενείς με τελικού σταδίου ΧΝΑ είναι πολυπαραγοντική καιαποδίδεται στην ουραιμία, την υποθρεψία, και την διαδικασία αιμοδιάλυσης, ηοποία μπορεί να επηρεάσει πολλαπλώς τόσο την εγγενή όσο και την επίκτητηανοσία. Η υπεργλυκαιμία είναι ένας καλά μελετημένος παράγοντας πουευοδώνει σε λοιμώδεις επιπλοκές. Η διαταραγμένη ομοιόσταση της γλυκόζηςεπηρεάζει πολλές παραμέτρους της ανοσιακής απάντησης,συμπεριλαμβανομένης της εκκρίσεως κυτταροκινών, της λειτουργίας των κυττάρων της ανοσίας και της επιθηλιακής δυσπραγίας. Οι ασθενείς με ΣΔείναι συνήθως εξαιρετικά επιρρεπείς σε λοιμώξεις.Αμέσως μετά μια μικροβιακή προσβολή η φλεγμονώδης απάντησηξεκινάει μετά την αναγνώριση των συστατικών του μικροβιακού εισβολέα. Οικύριες προφλεγμονώδεις κυτταροκίνες είναι ο TNF-α και οι ιντερλευκίνεςIL-6,IL-1β, and IL-8, οι οποίες προωθούν την φλεγμονώδη αντίδραση καιπροκαλούν την εμφάνιση του Συνδρόμου Συστηματικής ΦλεγμονώδουςΑντίδρασης {Systemic Inflammatory Response Syndrome (SIRS)}.Ταυτόχρονα με την προφλεγμονώδη διέγερση, ξεκινάει και ηαντιφλεγμονώδης, η οποία αντιπροσωπεύεται κυρίως από την IL-10. Ηκυριαρχία του προφλεγμονώδους ή του αντιφλεγμονώδους προφίλ σχετίζεταιμε την κλινική έκφραση της λοίμωξης και την έκβαση της σήψης αμφότερα.Η παθογένεση της gram(-) σήψης έχει μελετηθεί εκτεταμένως καιαποδίδεται σχεδόν αποκλειστικά στη δράση του λιποπολυσακχαρίτη(ενδοτοξίνη, LPS) του βακτηριακού τοιχώματος. Το κυτταρικό τοίχωμα τωνμυκήτων αποτελείται από τρείς κυρίως ομάδες πολυσακχαριδών, πολυμερήτης μανόζης (μαννοπρωτεΐνες, 40% της ξηρής μάζας του κυτταρικούτοιχώματος), πολυμερή της γλυκόζης (b-γλυκάνη, 60% της ξηρής μάζας τουκυτταρικού τοιχώματος) και πολυμερή της N-acetylglucosamine (χιτίνη, 2%της ξηρής μάζας του κυτταρικού τοιχώματος). Ανάμεσα σε αυτά οι μαννάνεςέχουν ιδιαίτερη σημαντικότητα, καθώς παρέχουν αντιγονική πολυμορφία καικατέχουν ανοσοδιεγερτικές ιδιότητες.Παρότι είναι βολικό να πιστεύουμε ότι μια κοινή παθογενετική οδόςαποτελεί την βάση σε όλες τις σηπτικές προσβολές, φαίνεται να υπάρχειδιαφορετικότητα ανάμεσα σε διαφορετικά είδη βακτηρίων. Σκοπός της μελέτης: Ο κύριος σκοπός της παρούσης μελέτης είναι νααξιολογήσει την βασική ανοσιακή κατάσταση των ασθενών των ευπαθών σεμυκητιασικές λοιμώξεις, και να αξιολογήσει την ανοσολογική τους απάντησημετά διέγερση με συστατικά του κυτταρικού τοιχώματος των μυκήτων και νασυγκριθεί αυτή η αντίδραση με την αντίδραση υγειών εθελοντών ενηλίκων,χρησιμοποιώντας ένα ex vivo μοντέλο διέγερσης. Ένας δευτερεύων σκοπόςήταν να διαπιστωθεί το εάν αυτέ οι ομάδες ασθενών ευρίσκονται σεκατάσταση ανοσοπαράλυσης, κάτι που αναγνωρίζεται με ολοένα καιαυξανόμενη συχνότητα σε βαριά πάσχοντες ασθενείς.Υλικό και μέθοδος: Μετρήσαμε τα βασικά επίπεδα των κυτταροκινών τουορού καθώς και τα επίπεδα που παρήχθησαν αυτές μετά από ex vivoδιέγερση με μαννάνη σε ολικό αίμα που συνελέγη από 10 υγιείς εθελοντές, 10ασθενείς με τελικού σταδίου ΧΝΑ, 10 ασθενείς με ΣΔ και 10 ασθενείς πουβρίσκονταν στη 2η ημέρα νοσηλείας τους σε ΜΕΘ, οι οποίοι είχαν μη σηπτικόSIRS και είχαν ένα APACHE II score ≥25. Χρησιμοποιήσαμε 100 μg/mlμαννάνης για μια περίοδο επώασης 8 ωρών ώστε να διεγείρουμε 1 ml ολικούαίματος. Τα δείγματα του αίματος συνελέγησαν από περιφερική φλέβα και ηδιέγερση έλαβε χώρα κάτω από τις ίδιες συνθήκες για όλες τις ομάδες μεμαννάνη από Saccharomyces cerevisiae της εταιρείας Sigma Chemical Co.(St Louis, MO, USA). Η μέτρηση των κυτταροκινών έγινε χρησιμοποιώνταςειδικά για το ανθρώπινο είδος εμπορικά διαθέσιμα κιτ με ELISA γιακυτταροκίνες.Αποτελέσματα: Όλες οι ομάδες των ασθενών είχαν υψηλότερες βασικέςτιμές TNF-α, IL-6, IL-1β, και IL-10 συγκρινόμενες με την ομάδα ελέγχου, αλλάμόνο στους ασθενείς της ΜΕΘ οι διαφορές ήταν στατιστικά σημαντικές. Ο λόγος IL-10/IL-6 βρέθηκε 0.33, 0.22 και 0.96 αντίστοιχα στους υγιείς, τουςασθενείς με ΧΝΑ και τους ασθενείς με ΣΔ, και 1.32 για τους ασθενείς τηςΜΕΘ πριν την διέγερση και 0.22, 0.51, 1.21, 2.46 αντίστοιχα μετά τηνδιέγερση. Σε όλες τις εξετασθείσες ομάδες, τα επίπεδα των κυτταροκινώναυξήθηκαν σημαντικά μετά την διέγερση με την μαννάνη, αν και οι βαριάπάσχοντες ασθενείς εμφάνισαν σημαντικά μικρότερη μεταβολή. Δενδιαπιστώθηκε ανοσοπαράλυση σε όλες τις εξετασθείσες ομάδες, αν καιυπήρξε σχετική ανοσοπαράλυση στους ασθενείς με ΣΔ και στους ασθενείςτης ομάδας της ΜΕΘ σύμφωνα με τον λόγο IL-10/Il-6. Επιπλέον, σύμφωνα μετον λόγο IL-10/TNF-α, όλες οι ομάδες ασθενών έχουν υψηλότερη πιθανότηταθανάτου σε σύγκριση με τους υγιείς εθελοντές, με αυτούς της ομάδας τηςΜΕΘ στην κορυφή της επικινδυνότητας και ακολουθούμενους από τουςασθενείς της ομάδας του ΣΔ. Ο λόγος IL-10/TNF-α πριν και μετά από τηνδιέγερση ήταν 0.52/0.35, 0.77/0.64, 0.96/0.91 και 1.46/1.19 για τους υγιείςεθελοντές, την ομάδα της ΧΝΑ, τους ασθενείς με ΣΔ και την ομάδα της ΜΕΘαντίστοιχα.Συμπεράσματα: Οι βαρέως πάσχοντες ασθενείς και δευτερευόντως οιασθενείς με τελικού σταδίου ΧΝΑ καθώς και οι ασθενείς με ΣΔ βρίσκονται σεπροφλεγμονώδη κατάσταση. Η ανταπόκριση των ασθενών με ΧΝΑ και ΣΔστην διέγερση με μαννάνη κρίνεται ως ικανοποιητική και συγκρίνεται με τηναντίδραση των υγειών ενηλίκων, ενώ αυτή των βαρέως πασχόντωνδιαφοροποιείται, πιθανά εξαιτίας της διαφορετικής ανοσολογικής τουςκατάστασης. Οι ασθενείς της ΜΕΘ και δευτερευόντως οι ασθενείς με ΣΔ είχανσχετική ανοσοπαράλυση.

scholarly journals Therapeutic effect of Xuezhitong capsule on microvascular angina

2021 ◽  
Vol 20 (9) ◽  
pp. 1991-1997
Author(s):  
Xiaomei Ma ◽  
Dehui Yang ◽  
Weichun Shen ◽  
Wei Liang ◽  
Qian Lin
Keyword(s):  
Treatment Group ◽  
Therapeutic Effect ◽  
Conventional Treatment ◽  
Density Lipoprotein ◽  
Control Group ◽  
Vascular Endothelial ◽  
Beijing City ◽  
Microvascular Angina ◽  
Tnf Α ◽  
Hs Crp

Purpose: To determine the therapeutic effect of Xuezhitong capsule in patients with microvascular angina (MVA), and its impact on vascular endothelial function.Methods: In total, 172 MVA patients treated in Beijing City Fengtai District Nanyuan Hospital from September 2017 to September 2019 were selected and randomized into control group which received conventional treatment, and treatment group which received Xuezhitong capsules plus. There were 86 patients in each group. Therapeutic effect, levels of inflammatory factors, i.e., high-sensitivity C- reactive protein (hs-CRP), interleukin-(IL-6), tumor necrosis factor-α (TNF-α), and vascular endothelial factors such as nitric oxide (NO), thromboxane B2 (TXB2) and endothelin (ET), were determined.Results: Markedly higher total treatment effectiveness was observed in the treatment group than in the control group (89.53 % vs. 72.94 %; p < 0.05). In both groups, treatment reduced the levels of hs-CRP, IL-6, TNF-α, TXB2 and ET, but elevated NO, with better results for treatment group than the control group (p < 0.05). Better optimizations of total cholesterol (TC), triglycerides (TGs), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein (HDL) were observed in the treatment group, relative to the control group (p < 0.05). Patients in the treatment group experienced fewer (8.14%) adverse reactions than those in control group (21.18 %, p < 0.05).Conclusion: Xuezhitong capsule, when combined with conventional treatment, exerts high therapeutic effectiveness and safety in MVA patients by inhibiting inflammatory reactions, optimizing endothelialfunction, reducing blood lipid levels, and decreasing the incidence of cardiovascular adverse events. Thus, the combination therapy is a potentially superior therapeutic strategy to the conventional approach for the management of MVA patients.

scholarly journals Analysis of the clinical value of Baogan Lishui decoction and convetional western medicine in treating Hepatitis B cirrhosis refractory ascites

2018 ◽  
Vol 2 (4) ◽  
Author(s):  
Huaide Lin
Keyword(s):  
Chinese Medicine ◽  
Hepatitis B ◽  
Combination Treatment ◽  
Western Medicine ◽  
Clinical Effect ◽  
Refractory Ascites ◽  
Control Group ◽  
Observation Group ◽  
Quality And Safety ◽  
Clinical Value

Objective: To investigate the clinical effect of combination treatment of Chinese medicine Baogan Lishui decoction and western medicine on hepatitis B cirrhosis related refactory ascites.Method: Control group was given conventional western medicine therapy while the observation group was given same conventional western medicine therapy with additional of Chinese medicine Baogan Lishui decoction.Result: The total effective of clinical treatment on hepatitis B cirrhosis related refractory ascites in observation group was higher than that of the control group which was 88.89% compared to 71.11%, respectively and it was significant different (p<0.05).Conclusion: The combination treatment of Chinese medicine Baogao Lishui decoction and conventional western medicine can significantly increased the treatment effect on hepatitis B cirrhosis refractory ascites, and thus increases the quality and safety of life.

scholarly journals Fasudil ameliorated liposaccharide-induced acute kidney injury in mice by inhibiting NLRP3

2021 ◽  
Vol 20 (10) ◽  
pp. 2055-2062
Author(s):  
Xueqian Li ◽  
Chengzhi Zhao
Keyword(s):  
Acute Kidney Injury ◽  
Treatment Group ◽  
Cell Growth ◽  
Mesangial Cells ◽  
Kidney Injury ◽  
Inflammatory Factors ◽  
Control Group ◽  
Model Group ◽  
Blank Control Group ◽  
Tissue Homogenates

Purpose: To determine the influence of fasudil on LPS-mediated acute kidney injury (AKI) in mice.Methods: Healthy C57 mice (n = 140) of largely similar weight were used in this study. They were assigned to a treatment group (n = 40), a model group (n = 50), and a blank control group (n = 50). Mice in treatment and model groups were injected with lipopolysaccharide (LPS). In the treatment group, each mouse was injected intravenously with fasudil daily before the establishment of the mouse model of AKI. All mice were sacrificed 6 h after establishing the AKI model. Portions of the kidney from mice were used for preparation of tissue homogenates, while the remaining portions were subjected to primary culture. Transformed C3H Mouse Kidney-1 (TCMK1) and mesangial cells from mouse glomeruli (SV40-MES-13) cells were used for assays of cell growth and apoptosis. Blood samples were alsocollected from the mice. Thereafter, the levels of blood urea nitrogen (BUN) and creatinine (Cr) in kidney homogenates of the three groups were determined. Moreover, levels of NLRP3, nuclear factor kappa-B (NF-κB), toll-like receptor 4 (TLR4), tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β in the homogenates and blood were assayed. Cell growth and apoptosis were also measured.Results: The treatment group and model group showed higher levels of BUN and Cr than the control group, with a higher level observed in model mice than in the treatment mice. There were significantly higher relative levels of NF-κB, NLRP3 and TLR4 in treatment and model groups than in controls, with a higher level observed in model mice than in treatment mice. There were significantly higher concentrations of inflammatory factors in treatment and model mice groups than in control mice, with higher levels observed in model mice than in treatment mice. The TCMK1 and SV40-MES-13 cells in the two groups showed slower cell growth and stronger apoptosis than those in control group (p < 0.05).Conclusion: Fasudil relieved LPS-mediated AKI in mice by suppressing TLR4/NF-κB signal pathway and lowering NLRP3. Thus, fasudil has potential as a new adjunctive agent for the treatment of AKI.

scholarly journals Preoperative pain aggravates postoperative cognitive deficits and hippocampal neuroinflammation in rats

2019 ◽  
Author(s):  
Xiang Gao ◽  
Xian Ding ◽  
Huan Yi ◽  
Chuan-tao Lin ◽  
Yu-ping Wang ◽  
...  
Keyword(s):  
Cognitive Function ◽  
Inflammatory Factors ◽  
Control Group ◽  
Gamma Aminobutyric Acid ◽  
Sprague Dawley ◽  
Preoperative Pain ◽  
Pain Model ◽  
Simple Operation ◽  
Tnf Α ◽  
Operation Group

Abstract Background Perioperative neurocognitive disorder (PND) is the progressive deterioration of cognitive function after surgery. The purpose of this study was to observe the effect of preoperative pain on inflammatory factors and neuronal apoptosis in the hippocampus of rats. Methods 36 adult male Sprague-Dawley rats were randomly divided into 4 groups: the control group, the pain group, the pain+operation group, and the operation group. 6 days before the surgery, the rats received cognitive training, and the cognitive evaluation was carried out on the1, 3 and 7th days after the surgery. The rats were killed on the first, third and seventh days after the surgery (n = 3 rats/day). The cognitive function of rats was evaluated by the Morris Water Maze (MWM), and the expression levels of the pro-inflammatory cytokines interleukin 6(IL-6), Interleukin 1β(IL-1β)and Tumor Necrosis Factor-α(TNF-α), Acetylcholine(Ach)and Cyclic Adenosine monophosphate(cAMP), protein kinase A(PKA)and gamma-aminobutyric acid type A receptors(GABAA) in the hippocampus were measured on the 1st, 3rd and 7th days after the operation. Results Our results showed that the pain model rats exhibited impaired behavior on the first day (P< 0.001), and this lasted until the 7th day after the operation (P≤0.002 and P≤0. 001, respectively). Preoperative pain model rats showed a higher level of apoptosis than that shown by the simple operation rats. On the 1st, 3rd and 7th days after the operation, the protein content of IL-1β, IL-6 and TNF-α in the pain operation group was increased compared to that in the simple operation group (P<0.001). ACh, cAMP, PKA and GABAA expression in the hippocampus was decreased after operation in the preoperative pain model rats. Conclusion Preoperative pain is a key risk factor for the development of PND. The ACh-PKA-GABAA signaling pathway plays a key role in the acetylcholine pathway.

scholarly journals Aberrant miR-219-5p is correlated with TLR4 and serves as a novel biomarker in patients with multiple organ dysfunction syndrome caused by acute paraquat poisoning

2020 ◽  
Vol 34 ◽  
pp. 205873842097488
Author(s):  
Yunxiang Dai ◽  
Xia Liu ◽  
Yuming Gao
Keyword(s):  
Organ Dysfunction ◽  
Multiple Organ Dysfunction Syndrome ◽  
Multiple Organ ◽  
Apache Ii ◽  
Luciferase Reporter ◽  
Apache Ii Score ◽  
Multiple Organ Dysfunction ◽  
Cox Regression Analysis ◽  
Potential Biomarker ◽  
Tnf Α

This study aimed to investigate the clinical significance of serum microRNA-219-5p (miR-219-5p) in patients with multiple organ dysfunction syndrome (MODS) caused by acute paraquat (PQ) poisoning, and its correlation with Toll-like Receptor 4 (TLR4). Luciferase reporter assay was used to investigate in vitro the correlation of miR-219-5p with TLR4. Serum miR-219-5p levels were evaluated by quantitative real-time polymerase chain reaction. Serum levels of TLR4, IL-1β, and TNF-α were measured by Enzyme-linked immune sorbent assay (ELISA). ROC analysis was performed to assess the diagnostic significance, Kaplan-Meier survival curves and Cox regression analysis were used to evaluate the prognostic value of miR-219-5p in MODS patients. TLR4 was a target gene of miR-219-5p and was increased in MODS patients. Serum miR-219-5p level was decreased and negatively correlated with TLR4 level in MODS patients ( r = −0.660, P < 0.001), which had important diagnostic value and negatively correlated with APACHE II score in MODS patients. The miR-219-5p expression was markedly associated with the WBC, ALT, AST, PaCO2, Lac, and APACHE II score. Non-survivals had more patients with low miR-219-5p expression. Patients with low miR-219-5p expression had shorter survival time. MiR-219-5p and APACHE II score were two independently prognostic factors for 28-day survival. MiR-219-5p was negatively correlated with, while TLR4 was positively correlated with the levels of IL-1β and TNF-α. The serum miR-219-5p level may be a potential biomarker for acute PQ-induced MODS diagnosis and prognosis. Furthermore, miR-219-5p may be associated with the progression of MODS by regulating TLR4-related inflammatory response.

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