scholarly journals A newly developed paper embedded microchip based on LAMP for rapid multiple detections of foodborne pathogens

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mimi Zhang ◽  
Jinfeng Liu ◽  
Zhiqiang Shen ◽  
Yongxin Liu ◽  
Yang Song ◽  
...  
Keyword(s):  
Centrifugal Force ◽  
Foodborne Pathogens ◽  
Pathogen Detection ◽  
Point Of Care ◽  
Foodborne Pathogen ◽  
Cost Effective ◽  
Reaction Chamber ◽  
Salmonella Spp ◽  
Culture Methods ◽  
Detection Technology

Abstract Background Microfluidic chip detection technology is considered a potent tool for many bioanalytic applications. Rapid detection of foodborne pathogens in the early stages is imperative to prevent the outbreak of foodborne diseases, known as a severe threat to human health. Conventional bacterial culture methods for detecting foodborne pathogens are time-consuming, laborious, and lacking in pathogen diagnosis. To overcome this problem, we have created an embedded paper-based microchip based on isothermal loop amplification (LAMP), which can rapidly and sensitively detect foodborne pathogens. Results We embed paper impregnated with LAMP reagent and specific primers in multiple reaction chambers of the microchip. The solution containing the target pathogen was injected into the center chamber and uniformly distributed into the reaction chamber by centrifugal force. The purified DNA of Escherichia coli O157:H7, Salmonella spp., Staphylococcus aureus, and Vibrio parahaemolyticus has been successfully amplified and directly detected on the microchip. The E. coli O157:H7 DNA was identified as low as 0.0134 ng μL− 1. Besides, the potential of this microchip in point-of-care testing was further tested by combining the on-chip sample purification module and using milk spiked with Salmonella spp.. The pyrolyzed milk sample was filtered through a polydopamine-coated paper embedded in the inside of the sample chamber. It was transported to the reaction chamber by centrifugal force for LAMP amplification. Then direct chip detection was performed in the reaction chamber embedded with calcein-soaked paper. The detection limit of Salmonella spp. in the sample measured by the microchip was approximately 12 CFU mL− 1. Conclusion The paper embedded LAMP microchip offers inexpensive, user-friendly, and highly selective pathogen detection capabilities. It is expected to have great potential as a quick, efficient, and cost-effective solution for future foodborne pathogen detection.

Utilizing the Public GenomeTrakr Database for Foodborne Pathogen Traceback v1

2021 ◽  
Author(s):  
Ruth E Timme ◽  
Maria Sanchez ◽  
Marc Allard
Keyword(s):  
Real Time ◽  
Foodborne Pathogens ◽  
Pathogen Detection ◽  
Foodborne Pathogen ◽  
Reference Database ◽  
Surveillance Program ◽  
Environmental Isolates ◽  
The Public ◽  
The World ◽  
The Usa

This protocol outlines the all the steps necessary to become a GenomeTrakr data contributor. GenomeTrakr is an international genomic reference database of mostly food and environmental isolates from foodborne pathogens. The data and analyses are housed at the National Center for Biotechnology Information (NCBI), which is a database freely available to anyone in the world. The Pathogen Detection browser at NCBI computes daily cluster results adding the newly submitted data to the existing phylogenetic clusters of closely related genomes. Contributors to this database can see how their new isolates are related to the real-time foodborne pathogen surveillance program established in the USA and a few other countries, and at the same time adding valuable new data to the reference database. ------ Although originally published as a Chapter in Methods and Protocols, Foodborne Bacterial Pathogens, the protocol has since been adapted and split into four separate protocols all of which are contained in this collection.

scholarly journals Applications of Nanotechnology in Sensor-Based Detection of Foodborne Pathogens

Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 1966 ◽  
Author(s):  
Harsh Kumar ◽  
Kamil Kuča ◽  
Shashi Kant Bhatia ◽  
Kritika Saini ◽  
Ankur Kaushal ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Pathogen Detection ◽  
Foodborne Pathogen ◽  
Health Issues ◽  
Detection And Identification ◽  
Specificity And Sensitivity ◽  
Food Borne ◽  
Contaminated Food ◽  
Working Principle ◽  
Effective Use

The intake of microbial-contaminated food poses severe health issues due to the outbreaks of stern food-borne diseases. Therefore, there is a need for precise detection and identification of pathogenic microbes and toxins in food to prevent these concerns. Thus, understanding the concept of biosensing has enabled researchers to develop nanobiosensors with different nanomaterials and composites to improve the sensitivity as well as the specificity of pathogen detection. The application of nanomaterials has enabled researchers to use advanced technologies in biosensors for the transfer of signals to enhance their efficiency and sensitivity. Nanomaterials like carbon nanotubes, magnetic and gold, dendrimers, graphene nanomaterials and quantum dots are predominantly used for developing biosensors with improved specificity and sensitivity of detection due to their exclusive chemical, magnetic, mechanical, optical and physical properties. All nanoparticles and new composites used in biosensors need to be classified and categorized for their enhanced performance, quick detection, and unobtrusive and effective use in foodborne analysis. Hence, this review intends to summarize the different sensing methods used in foodborne pathogen detection, their design, working principle and advances in sensing systems.

Rapid and accurate detection of Escherichia coli O157:H7 in beef using microfluidic wax-printed paper-based ELISA

The Analyst ◽  
2020 ◽  
Vol 145 (8) ◽  
pp. 3106-3115 ◽  
Author(s):  
Yanan Zhao ◽  
Dexin Zeng ◽  
Chao Yan ◽  
Wei Chen ◽  
Jianluan Ren ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
Point Of Care ◽  
Foodborne Pathogen ◽  
Escherichia Coli O157 ◽  
Good Sensitivity ◽  
Point Of Care Testing ◽  
Accurate Detection ◽  
New Ideas ◽  
Sensitivity Specificity

Escherichia coli O157:H7 is a severe foodborne pathogen. Paper-based ELISA can rapidly and accurately detect E.coli O157:H7 in beef. The method has good sensitivity, specificity and repeatability. It is suitable for point-of-care testing and offers new ideas for the detection of other foodborne pathogens.

scholarly journals Current State of Biosensors Development and their Application in Foodborne Pathogen Detection

2021 ◽  
Author(s):  
LUPING XU ◽  
Xingjian Bai ◽  
Arun K. Bhunia
Keyword(s):  
Mammalian Cells ◽  
Pathogen Detection ◽  
Safety Concern ◽  
Point Of Care ◽  
Foodborne Pathogen ◽  
Disease Outbreaks ◽  
High Specificity ◽  
Bacterial Cells ◽  
Outbreak Investigations ◽  
Foodborne Pathogen Detection

Foodborne disease outbreaks continue to be a major public health and food safety concern. Ensuring the safety of food prior to retail distribution by testing products promptly can protect consumers from foodborne diseases. F ast, sensitive, and accurate detection tools are in great demand. Therefore, various approaches have been explored in the past few years to find a more effective way to incorporate antibodies, oligonucleotides, phages, and mammalian cells as signal transducers and analyte recognition probes on biosensor platforms. The ultimate goal is to achieve high specificity and low detection limits (1-100 bacterial cells or pico-nanogram levels of toxins). Besides, advancement in mammalian cells and bacteriophage-based sensors led to their ability to detect not only low levels of pathogens but also to differentiate live from dead ones. Combining different biotechnology platforms enabled practical utility and application of biosensors in foodborne pathogen detection. However, further rigorous testing of biosensors from complex food matrices is needed to ensure their utility in point-of-care need and for outbreak investigations.

MicroSEQ® Salmonella spp. Detection Kit Using the Pathatrix® 10-Pooling Salmonella spp. Kit Linked Protocol Method Modification

2014 ◽  
Vol 97 (2) ◽  
pp. 484-491 ◽  
Author(s):  
Jason Wall ◽  
Rick Conrad ◽  
Kathy Latham ◽  
Eric Liu
Keyword(s):  
Real Time ◽  
Study Design ◽  
Foodborne Pathogens ◽  
Real Time Pcr ◽  
Reference Method ◽  
Immunomagnetic Separation ◽  
Pcr Analysis ◽  
Salmonella Spp ◽  
Culture Methods ◽  
Design For All

Abstract Real-time PCR methods for detecting foodborne pathogens offer the advantages of simplicity and quick time to results compared to traditional culture methods. The addition of a recirculating pooled immunomagnetic separation method prior to real-time PCR analysis increases processing output while reducing bothcost and labor. This AOAC Research Institute method modification study validates the MicroSEQ®Salmonella spp. Detection Kit [AOAC Performance Tested Method(PTM) 031001] linked with the Pathatrix® 10-Pooling Salmonella spp. Kit (AOAC PTM 090203C) in diced tomatoes, chocolate, and deli ham. The Pathatrix 10-Pooling protocol represents a method modification of the enrichment portion of the MicroSEQ Salmonella spp. protocol. The results of the method modification were compared to standard cultural reference methods for diced tomatoes, chocolate, and deli ham. All threematrixes were analyzed in a paired study design. An additional set of chocolate test portions was analyzed using an alternative enrichment medium in an unpaired study design. For all matrixes tested, there were no statistically significant differences in the number of positive test portions detected by the modified candidate method compared to the appropriate reference method. The MicroSEQ Salmonella spp. protocol linked with the Pathatrix individual or 10-Pooling procedure demonstrated reliabilityas a rapid, simplified, method for the preparation of samples and subsequent detection of Salmonella in diced tomatoes, chocolate, and deliham.

scholarly journals Transportation protocols for accurate assessment of microbial burden classification using molecular methods

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Amelia Kung ◽  
Jade Chen ◽  
Michael Tomasek ◽  
Dakai Liu ◽  
William Rodgers ◽  
...  
Keyword(s):  
Pathogen Detection ◽  
Point Of Care ◽  
Cost Effective ◽  
Optimal Growth ◽  
Testing Procedure ◽  
Initial Development ◽  
Specimen Collection ◽  
Skin Flora ◽  
Patient Side ◽  
Urine Specimens

AbstractPoint-of-care testing is cost-effective, rapid, and could assist in avoiding hospital visits during a pandemic. However, they present some significant risks that current technologies cannot fully address. Skin flora contamination and insufficient specimen volume are two major limitations preventing self-collection microbiological testing outside of hospital settings. We are developing a hybrid testing procedure to bridge the laboratory test with patient-side specimen collection and transportation for molecular microbial classification of causative bacterial infection and early identification of microbial susceptibility profiles directly from whole blood or urine specimens collected patient-side by health care workers such as phlebotomists in nursing homes or family clinics. This feasibility study presents our initial development efforts, in which we tested various transportation conditions (tubes, temperature, duration) for direct-from-specimen viable pathogen detection to determine the ideal conditions that allowed for differentiation between contaminant and causative bacteria in urine specimens and optimal growth for low-concentration blood specimens after transportation. For direct-from-urine assays, the viable pathogen at the clinical cutoff of 105 CFU/mL was detected after transportation with molecular assays while contaminants (≤ 104 CFU/mL) were not. For direct-from-blood assays, contrived blood samples as low as 0.8 CFU/mL were reported positive after transportation without the need for blood culture.

scholarly journals Advances in Nanomaterials-Based Electrochemical Biosensors for Foodborne Pathogen Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2700
Author(s):  
Ivan Bobrinetskiy ◽  
Marko Radovic ◽  
Francesco Rizzotto ◽  
Priya Vizzini ◽  
Stefan Jaric ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Foodborne Pathogens ◽  
Pathogen Detection ◽  
Carbon Nanomaterials ◽  
Graphene Quantum Dots ◽  
Foodborne Pathogen ◽  
Electrochemical Biosensors ◽  
Reduced Graphene ◽  
Gold Silver ◽  
2D And 3D Nanostructures

Electrochemical biosensors utilizing nanomaterials have received widespread attention in pathogen detection and monitoring. Here, the potential of different nanomaterials and electrochemical technologies is reviewed for the development of novel diagnostic devices for the detection of foodborne pathogens and their biomarkers. The overview covers basic electrochemical methods and means for electrode functionalization, utilization of nanomaterials that include quantum dots, gold, silver and magnetic nanoparticles, carbon nanomaterials (carbon and graphene quantum dots, carbon nanotubes, graphene and reduced graphene oxide, graphene nanoplatelets, laser-induced graphene), metal oxides (nanoparticles, 2D and 3D nanostructures) and other 2D nanomaterials. Moreover, the current and future landscape of synergic effects of nanocomposites combining different nanomaterials is provided to illustrate how the limitations of traditional technologies can be overcome to design rapid, ultrasensitive, specific and affordable biosensors.

scholarly journals A newly developed polydopamine-coated paper-based microchip for rapid and highly selectivity detection of foodborne pathogens

2021 ◽  
Author(s):  
Jinfeng Liu ◽  
Mimi Zhang ◽  
Zhiqiang Shen ◽  
Yongxin Liu ◽  
Yang Song ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Pathogen Detection ◽  
Lower Cost ◽  
Limit Of Detection ◽  
Fluorescence Labeling ◽  
Economic Losses ◽  
Great Promise ◽  
Salmonella Spp ◽  
Coated Paper ◽  
E Coli

Abstract Background In 2020, Covid-19 pneumonia has had a great impact on human health in although the countries around the world, it brings serious threaten to people’s lives and resulted in serious economic losses. At the same time, a lot news about the detection of Covid-19 in food emerges endlessly, a rapid and high selectivity detection method or technology is in urgent need for its ability to help relevant departments effectively control the epidemic situation and ensuring people’s lives and property safety. In recent years, loop-mediated isothermal amplification (LAMP) has been certified as a quick and highly selective technique to detect foodborne microorganisms. Results In this paper, a newly developed microchip with polydopamine-coated paper based on LAMP was fabricated. This microchip consists of nine chambers for sampling and reactions, the targeted nucleic acid of foodborne pathogens was labeled by calcein fluorescence rather than SYBR. The microchip is advantageous of lower cost of materials and simple pretreated methods, and is easy to operate without the need for complex controlled fluid flow. The LAMP procedure and fluorescence detection of pathogens can be carried on the chip without opening the lid, preventing aerosol contamination and reducing the probability of false positives. In experiments, the LAMP reaction conditions including the optimal reaction temperature and reaction time are thoroughly discussed and have been executed for various foodborne bacteria samples, including Escherichia coli O157:H7 (E. coli O157:H7), Salmonella spp., Staphylococcus aureus (S. aureus), and Vibrio parahaemolyticus (V. parahaemolyticus). Testing of E. coli O157:H7 proved to be highly selective and sensitive (as low as 0.0134 ng µL− 1). Additionally, experimental test of real milk sample was figured, the complete detection duration time was within 68 min, the limit of detection(LOD) for Salmonella spp. was determined to be lower than 12 CFU mL− 1. Conclusion In summary, a newly developed LAMP microchip with polydopamine-coated and calcein fluorescence labeling paper-based provides a lower cost, easy to use, highly selective, and multiplexable pathogen detection capability with great promise as a rapid, highly efficient, and economical solution for future foodborne pathogen testing.

scholarly journals Emerging Point-of-care Technologies for Food Safety Analysis

Sensors ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 817 ◽  
Author(s):  
Jane Choi ◽  
Kar Yong ◽  
Jean Choi ◽  
Alistair Cowie
Keyword(s):  
Food Safety ◽  
Foodborne Pathogens ◽  
High Performance ◽  
Point Of Care ◽  
Cost Effective ◽  
Safety Analysis ◽  
Public Concern ◽  
Food Contaminants ◽  
Safety Issues ◽  
User Friendly

Food safety issues have recently attracted public concern. The deleterious effects of compromised food safety on health have rendered food safety analysis an approach of paramount importance. While conventional techniques such as high-performance liquid chromatography and mass spectrometry have traditionally been utilized for the detection of food contaminants, they are relatively expensive, time-consuming and labor intensive, impeding their use for point-of-care (POC) applications. In addition, accessibility of these tests is limited in developing countries where food-related illnesses are prevalent. There is, therefore, an urgent need to develop simple and robust diagnostic POC devices. POC devices, including paper- and chip-based devices, are typically rapid, cost-effective and user-friendly, offering a tremendous potential for rapid food safety analysis at POC settings. Herein, we discuss the most recent advances in the development of emerging POC devices for food safety analysis. We first provide an overview of common food safety issues and the existing techniques for detecting food contaminants such as foodborne pathogens, chemicals, allergens, and toxins. The importance of rapid food safety analysis along with the beneficial use of miniaturized POC devices are subsequently reviewed. Finally, the existing challenges and future perspectives of developing the miniaturized POC devices for food safety monitoring are briefly discussed.

scholarly journals Biosensor for detection of bacteria with probiotic potential and food pathogens

2020 ◽  
Vol 9 (1) ◽  
pp. 800-807
Keyword(s):  
Foodborne Pathogens ◽  
Pathogen Detection ◽  
Food Contamination ◽  
High Specificity ◽  
Nanostructured Material ◽  
Probiotic Bacteria ◽  
Food Contaminants ◽  
Probiotic Potential ◽  
Detection Technology ◽  
Wide Range

The exploration for novel nano-sensors has enhanced significantly representing an incredible alternative for the development, speedy, and inexpensive bio-sensing strategy. Due to their low detection volumes, reduction of detection time, high specificity and user- friendly applicability, nano-bio sensors have raised the interest of the scientific community. Nanomaterials are now being used to develop biosensors thatexhibit superior sensitivity and uniqueness with applicability in research investigations, food contamination detection, detection of potential probiotic bacteria, etc. Detection of food contamination is of major significance and concern in areas like healthcare, agriculture, beverage, and fermentation industries. Distinctive biosensing technologies have already been developed for instant monitoring of microbes, food contaminants depending upon the application of nanomaterial. A wide range of nanomaterials, for example, gold nanostructured materials, carbon Copper and silicon nanotubes, GeO2/SiO2 matrix, nanoparticles, nanowires, TiO2 nanowire, nano-electrode, and nanostructured material arrays are performing an essential role in the bio-sensing application in food pathogen detection and probiotic bacteria detection.Nanosensors merges the principles of information technology and molecular biology proves essential in facilitating immediate detection of foodborne pathogens, contaminants, hence reducing the health risk and medical costs related to foodborne illness.This chapter aims to encompass the types of emerging nanosensors based on different detection technology, their commercial applications, recent advancement in food contamination detection and their future prospects.

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