scholarly journals Applications of Nanotechnology in Sensor-Based Detection of Foodborne Pathogens

Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 1966 ◽  
Author(s):  
Harsh Kumar ◽  
Kamil Kuča ◽  
Shashi Kant Bhatia ◽  
Kritika Saini ◽  
Ankur Kaushal ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Pathogen Detection ◽  
Foodborne Pathogen ◽  
Health Issues ◽  
Detection And Identification ◽  
Specificity And Sensitivity ◽  
Food Borne ◽  
Contaminated Food ◽  
Working Principle ◽  
Effective Use

The intake of microbial-contaminated food poses severe health issues due to the outbreaks of stern food-borne diseases. Therefore, there is a need for precise detection and identification of pathogenic microbes and toxins in food to prevent these concerns. Thus, understanding the concept of biosensing has enabled researchers to develop nanobiosensors with different nanomaterials and composites to improve the sensitivity as well as the specificity of pathogen detection. The application of nanomaterials has enabled researchers to use advanced technologies in biosensors for the transfer of signals to enhance their efficiency and sensitivity. Nanomaterials like carbon nanotubes, magnetic and gold, dendrimers, graphene nanomaterials and quantum dots are predominantly used for developing biosensors with improved specificity and sensitivity of detection due to their exclusive chemical, magnetic, mechanical, optical and physical properties. All nanoparticles and new composites used in biosensors need to be classified and categorized for their enhanced performance, quick detection, and unobtrusive and effective use in foodborne analysis. Hence, this review intends to summarize the different sensing methods used in foodborne pathogen detection, their design, working principle and advances in sensing systems.

Fluorescence sensor arrays for identification of foodborne pathogens

2015 ◽  
Vol 7 (18) ◽  
pp. 7431-7435 ◽  
Author(s):  
Radeemada Mungkarndee ◽  
Gamolwan Tumcharern ◽  
Raweewan Thiramanus ◽  
Ittipon Techakriengkrai ◽  
Mongkol Sukwattanasinitt
Keyword(s):  
Foodborne Pathogens ◽  
Pathogen Detection ◽  
Sensor Array ◽  
Foodborne Pathogen ◽  
Sensor Arrays ◽  
Fluorescence Sensor ◽  
Detection And Identification ◽  
Foodborne Pathogen Detection

A fluorescence sensor array containing polyelectrolyte fluorophores has been developed for foodborne pathogen detection and identification.

Utilizing the Public GenomeTrakr Database for Foodborne Pathogen Traceback v1

2021 ◽  
Author(s):  
Ruth E Timme ◽  
Maria Sanchez ◽  
Marc Allard
Keyword(s):  
Real Time ◽  
Foodborne Pathogens ◽  
Pathogen Detection ◽  
Foodborne Pathogen ◽  
Reference Database ◽  
Surveillance Program ◽  
Environmental Isolates ◽  
The Public ◽  
The World ◽  
The Usa

This protocol outlines the all the steps necessary to become a GenomeTrakr data contributor. GenomeTrakr is an international genomic reference database of mostly food and environmental isolates from foodborne pathogens. The data and analyses are housed at the National Center for Biotechnology Information (NCBI), which is a database freely available to anyone in the world. The Pathogen Detection browser at NCBI computes daily cluster results adding the newly submitted data to the existing phylogenetic clusters of closely related genomes. Contributors to this database can see how their new isolates are related to the real-time foodborne pathogen surveillance program established in the USA and a few other countries, and at the same time adding valuable new data to the reference database. ------ Although originally published as a Chapter in Methods and Protocols, Foodborne Bacterial Pathogens, the protocol has since been adapted and split into four separate protocols all of which are contained in this collection.

One Health and Parasites

2019 ◽  
pp. 82-112
Author(s):  
Said Sajjad Ali Shah ◽  
Adnan Khan
Keyword(s):  
One Health ◽  
Health Workers ◽  
Animal Health ◽  
Health Issues ◽  
Food Borne ◽  
Contaminated Food ◽  
Parasitic Zoonoses ◽  
Public Health Workers ◽  
Etiological Agents ◽  
Water Borne

One health is a collective term used to address human and animal health issues under one platform. More than half of the diseases of humans are directly or indirectly related to animal health and spread from animals to humans or vice versa. Etiological agents of zoonotic diseases may be bacterial, viral, or parasitic in origin. Among them, parasitic agents are very important because they are either directly involved as etiological agents or as vectors of other pathogenic organisms. Parasitic zoonoses are transmitted to humans through vectors, food, or drinking water, and thus categorized as vector borne, food borne, and water borne parasitic zoonoses. Food borne and water borne parasitic zoonoses include all those parasitic diseases which are transmitted to humans by consuming contaminated food and water. An extensive alliance is necessary amongst physicians, veterinarians, and public health workers for timely response and approach to guarantee the prevention and management of infections.

scholarly journals A newly developed paper embedded microchip based on LAMP for rapid multiple detections of foodborne pathogens

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mimi Zhang ◽  
Jinfeng Liu ◽  
Zhiqiang Shen ◽  
Yongxin Liu ◽  
Yang Song ◽  
...  
Keyword(s):  
Centrifugal Force ◽  
Foodborne Pathogens ◽  
Pathogen Detection ◽  
Point Of Care ◽  
Foodborne Pathogen ◽  
Cost Effective ◽  
Reaction Chamber ◽  
Salmonella Spp ◽  
Culture Methods ◽  
Detection Technology

Abstract Background Microfluidic chip detection technology is considered a potent tool for many bioanalytic applications. Rapid detection of foodborne pathogens in the early stages is imperative to prevent the outbreak of foodborne diseases, known as a severe threat to human health. Conventional bacterial culture methods for detecting foodborne pathogens are time-consuming, laborious, and lacking in pathogen diagnosis. To overcome this problem, we have created an embedded paper-based microchip based on isothermal loop amplification (LAMP), which can rapidly and sensitively detect foodborne pathogens. Results We embed paper impregnated with LAMP reagent and specific primers in multiple reaction chambers of the microchip. The solution containing the target pathogen was injected into the center chamber and uniformly distributed into the reaction chamber by centrifugal force. The purified DNA of Escherichia coli O157:H7, Salmonella spp., Staphylococcus aureus, and Vibrio parahaemolyticus has been successfully amplified and directly detected on the microchip. The E. coli O157:H7 DNA was identified as low as 0.0134 ng μL− 1. Besides, the potential of this microchip in point-of-care testing was further tested by combining the on-chip sample purification module and using milk spiked with Salmonella spp.. The pyrolyzed milk sample was filtered through a polydopamine-coated paper embedded in the inside of the sample chamber. It was transported to the reaction chamber by centrifugal force for LAMP amplification. Then direct chip detection was performed in the reaction chamber embedded with calcein-soaked paper. The detection limit of Salmonella spp. in the sample measured by the microchip was approximately 12 CFU mL− 1. Conclusion The paper embedded LAMP microchip offers inexpensive, user-friendly, and highly selective pathogen detection capabilities. It is expected to have great potential as a quick, efficient, and cost-effective solution for future foodborne pathogen detection.

Application of Phage for Biocontrol of Salmonella Species in Food Systems

2020 ◽  
Vol 8 (10) ◽  
pp. 2214-2221
Author(s):  
Zehra Tuğçe Toprak ◽  
Pınar Şanlıbaba
Keyword(s):  
Foodborne Pathogens ◽  
Bacterial Cell ◽  
Dairy Products ◽  
Food Systems ◽  
Meat Products ◽  
Pathogenic Microorganisms ◽  
Food Borne ◽  
Contaminated Food ◽  
Seafood Products ◽  
Environmentally Friendly Approach

The studies on phage applications that provide successful results in biocontrol of foodborne pathogens and offer an environmentally friendly approach have been increasing today. Phages are viruses that can infect and kill the specific target bacterial cell. Salmonella is one of the most important pathogenic microorganisms that leading causes of food-borne illnesses called salmonellosis. Meat products especially chicken meat, fresh eggs, dairy products, ready-to-eat foods, seafood products and all kinds of contaminated food can be cause of salmonellosis. In this review, the phage application studies to control of Salmonella in food systems were summarized taking into account the research studied in recent years.

scholarly journals Advances in Nanomaterials-Based Electrochemical Biosensors for Foodborne Pathogen Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2700
Author(s):  
Ivan Bobrinetskiy ◽  
Marko Radovic ◽  
Francesco Rizzotto ◽  
Priya Vizzini ◽  
Stefan Jaric ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Foodborne Pathogens ◽  
Pathogen Detection ◽  
Carbon Nanomaterials ◽  
Graphene Quantum Dots ◽  
Foodborne Pathogen ◽  
Electrochemical Biosensors ◽  
Reduced Graphene ◽  
Gold Silver ◽  
2D And 3D Nanostructures

Electrochemical biosensors utilizing nanomaterials have received widespread attention in pathogen detection and monitoring. Here, the potential of different nanomaterials and electrochemical technologies is reviewed for the development of novel diagnostic devices for the detection of foodborne pathogens and their biomarkers. The overview covers basic electrochemical methods and means for electrode functionalization, utilization of nanomaterials that include quantum dots, gold, silver and magnetic nanoparticles, carbon nanomaterials (carbon and graphene quantum dots, carbon nanotubes, graphene and reduced graphene oxide, graphene nanoplatelets, laser-induced graphene), metal oxides (nanoparticles, 2D and 3D nanostructures) and other 2D nanomaterials. Moreover, the current and future landscape of synergic effects of nanocomposites combining different nanomaterials is provided to illustrate how the limitations of traditional technologies can be overcome to design rapid, ultrasensitive, specific and affordable biosensors.

scholarly journals Development of Simple Multiplex Real-Time PCR Assays for Foodborne Pathogens Detection and Identification On Lightcycler

2017 ◽  
Vol 40 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Avo Karus ◽  
Fabrizio Ceciliani ◽  
Armand Sanches Bonastre ◽  
Virge Karus
Keyword(s):  
Real Time ◽  
Foodborne Pathogens ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Melting Curve ◽  
Food Contamination ◽  
Shigella Boydii ◽  
Food Borne Pathogens ◽  
Detection And Identification ◽  
Food Borne

Abstract Most acute intestinal diseases are caused by food-borne pathogens. A fast and simple real-time PCR-based procedure for simultaneous detection of food contamination by any of the five food-borne pathogens: Campylobacter jejuni, Mycobacterium bovis, Enterobacter sakazaki, Shigella boydii, Clostridium perfrigens using multiplex EvaGreen real-time PCR for LightCycler was developed and evaluated. Real-time qPCR showed excellent sensitivity. Tm calling and Melting Curve Genotyping (MCG) were used for analysis of PCR product melting curves. The Melting Curve Genotyping option showed good performance for discrimination of positive samples containing DNA of single pathogen or pathogen mixtures from negative samples.

scholarly journals A Disposable and Multi-Chamber Film-Based PCR Chip for Detection of Foodborne Pathogen

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 3158 ◽  
Author(s):  
Nam Bae ◽  
Sun Lim ◽  
Younseong Song ◽  
Soon Jeong ◽  
Seol Shin ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Pathogen Detection ◽  
Detection System ◽  
Foodborne Pathogen ◽  
Pcr Amplification ◽  
Dna Amplification ◽  
Adhesive Film ◽  
Thermal Transfer ◽  
Polymerase Chain ◽  
Structurally Stable

Since the increment of the threat to public health caused by foodborne pathogens, researches have been widely studied on developing the miniaturized detection system for the on-site pathogen detection. In the study, we focused on the development of portable, robust, and disposable film-based polymerase chain reaction (PCR) chip containing a multiplex chamber for simultaneous gene amplification. In order to simply fabricate and operate a film-based PCR chip, different kinds of PCR chambers were designed and fabricated using polyethylene terephthalate (PET) and polyvinyl chloride (PVC) adhesive film, in comparison with commercial PCR, which employs a stereotyped system at a bench-top scale. No reagent leakage was confirmed during the PCR thermal cycling using the film PCR chip, which indicates that the film PCR chip is structurally stable for rapid heat cycling for DNA amplification. Owing to use of the thin film to fabricate the PCR chip, we are able to realize fast thermal transfer from the heat block that leads to short PCR amplification time. Moreover, using the film PCR chip, we could even amplify the target pathogen with 10 CFU mL−1. The artificially infected milk with various concentration of Bacillus cereus was successfully amplified on a single film PCR chip. On the basis of the reliable results, the developed film PCR chip could be a useful tool as a POCT device to detect foodborne pathogens via genetic analysis.

scholarly journals Microfluidic-Based Approaches for Foodborne Pathogen Detection

2019 ◽  
Vol 7 (10) ◽  
pp. 381 ◽  
Author(s):  
Xihong Zhao ◽  
Mei Li ◽  
Yao Liu
Keyword(s):  
Food Safety ◽  
Foodborne Pathogens ◽  
Pathogen Detection ◽  
Accurate Determination ◽  
Foodborne Pathogen ◽  
Current Status ◽  
Future Research ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Beings ◽  
Microfluidic Technology

Food safety is of obvious importance, but there are frequent problems caused by foodborne pathogens that threaten the safety and health of human beings worldwide. Although the most classic method for detecting bacteria is the plate counting method, it takes almost three to seven days to get the bacterial results for the detection. Additionally, there are many existing technologies for accurate determination of pathogens, such as polymerase chain reaction (PCR), enzyme linked immunosorbent assay (ELISA), or loop-mediated isothermal amplification (LAMP), but they are not suitable for timely and rapid on-site detection due to time-consuming pretreatment, complex operations and false positive results. Therefore, an urgent goal remains to determine how to quickly and effectively prevent and control the occurrence of foodborne diseases that are harmful to humans. As an alternative, microfluidic devices with miniaturization, portability and low cost have been introduced for pathogen detection. In particular, the use of microfluidic technologies is a promising direction of research for this purpose. Herein, this article systematically reviews the use of microfluidic technology for the rapid and sensitive detection of foodborne pathogens. First, microfluidic technology is introduced, including the basic concepts, background, and the pros and cons of different starting materials for specific applications. Next, the applications and problems of microfluidics for the detection of pathogens are discussed. The current status and different applications of microfluidic-based technologies to distinguish and identify foodborne pathogens are described in detail. Finally, future trends of microfluidics in food safety are discussed to provide the necessary foundation for future research efforts.

Specificity of the BAX Polymerase Chain Reaction System for Detection of the Foodborne Pathogen Listeria monocytogenes

1998 ◽  
Vol 81 (4) ◽  
pp. 817-822 ◽  
Author(s):  
Diana Stewart ◽  
Steven M Gendel
Keyword(s):  
Polymerase Chain Reaction ◽  
Listeria Monocytogenes ◽  
Foodborne Pathogens ◽  
Foodborne Pathogen ◽  
Reaction System ◽  
Chain Reaction ◽  
Mixed Cell ◽  
Specificity And Sensitivity ◽  
Colony Forming Units ◽  
Polymerase Chain

Abstract The polymerase chain reaction (PCR) can be used for rapid and specific detection of foodborne pathogens. One commercial kit, the Qualicon BAX system uses PCR to detect Listeria monocytogenes in enrichment cultures derived from food and environmental samples. The specificity and sensitivity of the BAX system for detecting L. monocytogenes were characterized by using both pure and mixed cell cultures, and optimal conditions for production of cell lysates were determined. The BAX system was highly specific for L. monocytogenes, and no interference was seen in the presence of either other Listeria species or microbes from other genera. The assay detected L. monocytogenes at 105- 106 colony-forming units/mL. This sensitivity is adequate for detecting viable cells after enrichment but prevents false-positive signals from nonviable cells.

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