scholarly journals Insight into transketolase of Pyropia haitanensis under desiccation stress based on integrative analysis of omics and transformation

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Jianzhi Shi ◽  
Wenlei Wang ◽  
Yinghui Lin ◽  
Kai Xu ◽  
Yan Xu ◽  
...  
Keyword(s):  
Desiccation Tolerance ◽  
Integrative Analysis ◽  
Pyropia Haitanensis ◽  
Desiccation Stress ◽  
Integrated Analysis ◽  
Antioxidant Systems ◽  
Water Losses ◽  
Harsh Conditions ◽  
Insight Into ◽  
Better Than

Abstract Background Pyropia haitanensis, distributes in the intertidal zone, can tolerate water losses exceeding 90%. However, the mechanisms enabling P. haitanensis to survive harsh conditions remain uncharacterized. To elucidate the mechanism underlying P. haitanensis desiccation tolerance, we completed an integrated analysis of its transcriptome and proteome as well as transgenic Chlamydomonas reinhardtii carrying a P. haitanensis gene. Results P. haitanensis rapidly adjusted its physiological activities to compensate for water losses up to 60%, after which, photosynthesis, antioxidant systems, chaperones, and cytoskeleton were activated to response to severe desiccation stress. The integrative analysis suggested that transketolase (TKL) was affected by all desiccation treatments. Transgenic C. reinhardtii cells overexpressed PhTKL grew better than the wild-type cells in response to osmotic stress. Conclusion P. haitanensis quickly establishes acclimatory homeostasis regarding its transcriptome and proteome to ensure its thalli can recover after being rehydrated. Additionally, PhTKL is vital for P. haitanensis desiccation tolerance. The present data may provide new insights for the breeding of algae and plants exhibiting enhanced desiccation tolerance.

scholarly journals The Effectiveness of Multi-Label Classification and Multi-Output Regression in Social Trait Recognition

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 4127
Author(s):  
Will Farlessyost ◽  
Kelsey-Ryan Grant ◽  
Sara R. Davis ◽  
David Feil-Seifer ◽  
Emily M. Hand
Keyword(s):  
Social Interactions ◽  
First Impressions ◽  
Social Traits ◽  
Judicial Proceedings ◽  
Facial Attributes ◽  
Future Work ◽  
Automated Methods ◽  
Insight Into ◽  
Social Trait ◽  
Better Than

First impressions make up an integral part of our interactions with other humans by providing an instantaneous judgment of the trustworthiness, dominance and attractiveness of an individual prior to engaging in any other form of interaction. Unfortunately, this can lead to unintentional bias in situations that have serious consequences, whether it be in judicial proceedings, career advancement, or politics. The ability to automatically recognize social traits presents a number of highly useful applications: from minimizing bias in social interactions to providing insight into how our own facial attributes are interpreted by others. However, while first impressions are well-studied in the field of psychology, automated methods for predicting social traits are largely non-existent. In this work, we demonstrate the feasibility of two automated approaches—multi-label classification (MLC) and multi-output regression (MOR)—for first impression recognition from faces. We demonstrate that both approaches are able to predict social traits with better than chance accuracy, but there is still significant room for improvement. We evaluate ethical concerns and detail application areas for future work in this direction.

scholarly journals Acoustic-Prosodic and Lexical Cues to Deception and Trust: Deciphering How People Detect Lies

2020 ◽  
Vol 8 ◽  
pp. 199-214
Author(s):  
Xi (Leslie) Chen ◽  
Sarah Ita Levitan ◽  
Michelle Levine ◽  
Marko Mandic ◽  
Julia Hirschberg
Keyword(s):  
Deception Detection ◽  
Human Perception ◽  
Lie Detection ◽  
Challenging Problem ◽  
Cues To Deception ◽  
Lexical Cues ◽  
Insight Into ◽  
Large Corpus ◽  
Better Than

Humans rarely perform better than chance at lie detection. To better understand human perception of deception, we created a game framework, LieCatcher, to collect ratings of perceived deception using a large corpus of deceptive and truthful interviews. We analyzed the acoustic-prosodic and linguistic characteristics of language trusted and mistrusted by raters and compared these to characteristics of actual truthful and deceptive language to understand how perception aligns with reality. With this data we built classifiers to automatically distinguish trusted from mistrusted speech, achieving an F1 of 66.1%. We next evaluated whether the strategies raters said they used to discriminate between truthful and deceptive responses were in fact useful. Our results show that, although several prosodic and lexical features were consistently perceived as trustworthy, they were not reliable cues. Also, the strategies that judges reported using in deception detection were not helpful for the task. Our work sheds light on the nature of trusted language and provides insight into the challenging problem of human deception detection.

The Military Physician in Captivity

1969 ◽  
Vol 9 (99) ◽  
pp. 295-303
Author(s):  
E. Reginato
Keyword(s):  
Medical Officer ◽  
Red Cross ◽  
Prisoner Of War ◽  
Geneva Conventions ◽  
Peace And Justice ◽  
In Captivity ◽  
Military Physician ◽  
The Military ◽  
Insight Into ◽  
Better Than

In his introductory address at the third International Refresher Course for Junior Medical Officers, Dr. H. Meuli, member of the ICRC, said “No one knows war better than the military medical officer, nor measures its horror, nor hates it more. No one has greater insight into war to enable him to take a stand for peace and against war”. From its very beginnings the Red Cross has been linked to medicine; it was the ICRC which obtained for doctors the means of exercising their profession in war, which are laid down in the Geneva Conventions.It therefore seems appropriate to quote extensively from a communication submitted at the Course by an Italian doctor, bearing moving testimony to the difficulties facing the medical officer, the noble character of his mission and the principles underlying his activity in the prisoner of war camp. These principles were summed up in his conclusion : “Like peace and justice, medicine loses its significance if not accompanied by charity. If it is to stay universal, it must not lose its humanity”. (Ed.).

scholarly journals Design and Demonstration of Large Scale Cu2O Photocathodes with Metal Grid Structure for Photoelectrochemical Water Splitting

Energies ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 7422
Author(s):  
Min-Kyu Son
Keyword(s):  
Water Splitting ◽  
Large Scale ◽  
Grid Structure ◽  
Large Area ◽  
Intrinsic Conductivity ◽  
Metal Grid ◽  
Splitting System ◽  
Insight Into ◽  
Better Than

Upscaling of photoelectrode for a practical photoelectrochemical (PEC) water splitting system is still challenging because the PEC performance of large-scale photoelectrode is significantly low, compared to the lab scale photoelectrode. In an effort to overcome this challenge, sputtered gold (Au) and copper (Cu) grid lines were introduced to improve the PEC performance of large-scale cuprous oxide (Cu2O) photocathode in this work. It was demonstrated that Cu grid lines are more effective than Au grid lines to improve the PEC performance of large-scale Cu2O photocathode because its intrinsic conductivity and quality of grid lines are better than ones containing Au grid lines. As a result, the PEC performance of a 25-cm2 scaled Cu2O photocathode with Cu grid lines was almost double than one without grid lines, resulting in an improved charge transport in the large area substrate by Cu grid lines. Finally, a 50-cm2 scaled Cu2O photocathode with Cu grid lines was tested in an outdoor condition under natural sun. This is the first outdoor PEC demonstration of large-scale Cu2O photocathode with Cu grid lines, which gives insight into the development of efficient upscaled PEC photoelectrode.

scholarly journals Asymmetries in mutual understanding: People with low status, power, and self-esteem understand better than they are understood

2020 ◽  
Author(s):  
Sanaz Talaifar ◽  
Michael Buhrmester ◽  
Ozlem Ayduk ◽  
william swann
Keyword(s):  
Low Power ◽  
Conceptual Analysis ◽  
Self Esteem ◽  
Mutual Understanding ◽  
Insight Into ◽  
Better Than

NOW PUBLISHED: https://doi.org/10.1177/1745691620958003. All too often, people who develop exceptionally astute insights into others remain mysterious to these others. Evidence for such asymmetric understanding comes from several independent domains. Striking asymmetries occur among those who differ in status and power, such that low status, low power individuals understand more than they are understood. We show that this effect extends to people who merely perceive that they have low status: individuals with low self-esteem. Whereas people with low self-esteem display insight into people with high self-esteem, their high self-esteem counterparts fail to reciprocate. Conceptual analysis suggests that asymmetries in mutual understanding may be reduced by addressing deficits in information and motivation among perceivers. Nevertheless, evidence from several interventions were unsuccessful, indicating that the path to symmetric understanding is a steep and thorny one. Further research is needed to develop strategies for fostering understanding of those who are most misunderstood: people with low self-esteem, low status, and low power.

scholarly journals Extremophilic Prokaryotic Endoxylanases: Diversity, Applicability, and Molecular Insights

2021 ◽  
Vol 12 ◽  
Author(s):  
Digvijay Verma
Keyword(s):  
Molecular Mechanisms ◽  
Good Choice ◽  
Industrial Processes ◽  
Low Molecular Weight ◽  
Short Life Span ◽  
Functional Attributes ◽  
Extreme Behavior ◽  
Harsh Conditions ◽  
Free Activity ◽  
Better Than

Extremophilic endoxylanases grabbed attention in recent years due to their applicability under harsh conditions of several industrial processes. Thermophilic, alkaliphilic, and acidophilic endoxylanases found their employability in bio-bleaching of paper pulp, bioconversion of lignocellulosic biomass into xylooligosaccharides, bioethanol production, and improving the nutritious value of bread and other bakery products. Xylanases obtained from extremophilic bacteria and archaea are considered better than fungal sources for several reasons. For example, enzymatic activity under broad pH and temperature range, low molecular weight, cellulase-free activity, and longer stability under extreme conditions of prokaryotic derived xylanases make them a good choice. In addition, a short life span, easy cultivation/harvesting methods, higher yield, and rapid DNA manipulations of bacterial and archaeal cells further reduces the overall cost of the product. This review focuses on the diversity of prokaryotic endoxylanases, their characteristics, and their functional attributes. Besides, the molecular mechanisms of their extreme behavior have also been presented here.

scholarly journals Informative Frailty Indices from Binarized Biomarkers

2020 ◽  
Author(s):  
Garrett Stubbings ◽  
Spencer Farrell ◽  
Arnold Mitnitski ◽  
Kenneth Rockwood ◽  
Andrew Rutenberg
Keyword(s):  
Medical Knowledge ◽  
Data Sets ◽  
Canadian Study ◽  
Adverse Health Outcomes ◽  
Health And Nutrition ◽  
Health Measure ◽  
Upper Limits ◽  
Insight Into ◽  
Better Than ◽  
Biomarker Data

AbstractFrailty indices (FI) based on continuous valued health data, such as obtained from blood and urine tests, have been shown to be predictive of adverse health outcomes. However, creating FI from such biomarker data requires a binarization treatment that is difficult to standardize across studies. In this work, we explore a “quantile” methodology for the generic treatment of biomarker data that allows us to construct an FI without preexisting medical knowledge (i.e. risk thresholds) of the included biomarkers. We show that our quantile approach performs as well as, or even slightly better than, established methods for the National Health and Nutrition Examination Survey (NHANES) and the Canadian Study of Health and Aging (CSHA) data sets. Furthermore, we show that our approach is robust to cohort effects within studies as compared to other data-based methods. The success of our binarization approaches provides insight into the robustness of the FI as a health measure, the upper limits of the FI observed in various data sets, and highlights general difficulties in obtaining absolute scales for comparing FI between studies.

Chemotherapy of drug-resistant ovarian cancer: a Southwest Oncology Group Study.

1986 ◽  
Vol 4 (9) ◽  
pp. 1374-1379 ◽  
Author(s):  
L R Laufman ◽  
J B Green ◽  
D S Alberts ◽  
R O'Toole ◽  
R D Hilgers ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Response Rate ◽  
Final Analysis ◽  
Drug Resistant ◽  
Eligibility Criteria ◽  
Interim Analyses ◽  
Southwest Oncology Group Study ◽  
Pathology Review ◽  
Insight Into ◽  
Better Than

We present a final analysis, including pathology review, of a cooperative group study of drug-resistant ovarian cancer. Of 200 patients registered, 112 were eligible and evaluable, with a response rate of 26% and median survival of 7 months. Because these results are poorer than those reported in the preliminary and interim analyses of this study, we scrutinized the 88 excluded patients, most of whom failed to meet our strict pathologic criteria for a diagnosis of ovarian cancer of epithelial type, and who, as a heterogeneous group, fared better than patients who did meet the eligibility criteria. We believe this analysis provides insight into the spectrum of diseases that are frequently called ovarian cancer, but might be more properly labeled abdominal carcinomatosis.

scholarly journals Unique MicroRNA and mRNA Interactions in EGFR-Mutated Lung Adenocarcinoma

2018 ◽  
Vol 7 (11) ◽  
pp. 419 ◽  
Author(s):  
Sophia Subat ◽  
Kentaro Inamura ◽  
Hironori Ninomiya ◽  
Hiroko Nagano ◽  
Sakae Okumura ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Mrna Expression ◽  
Regulatory Network ◽  
Egfr Mutation ◽  
Expression Profiles ◽  
Integrative Analysis ◽  
Future Research ◽  
Mutation Status ◽  
Egfr Mutated ◽  
Insight Into

The EGFR gene was one of the first molecules to be selected for targeted gene therapy. EGFR-mutated lung adenocarcinoma, which is responsive to EGFR inhibitors, is characterized by a distinct oncogenic pathway in which unique microRNA (miRNA)–mRNA interactions have been observed. However, little information is available about the miRNA–mRNA regulatory network involved. Both miRNA and mRNA expression profiles were investigated using microarrays in 155 surgically resected specimens of lung adenocarcinoma with a known EGFR mutation status (52 mutated and 103 wild-type cases). An integrative analysis of the data was performed to identify the unique miRNA–mRNA regulatory network in EGFR-mutated lung adenocarcinoma. Expression profiling of miRNAs and mRNAs yielded characteristic miRNA/mRNA signatures (19 miRNAs/431 mRNAs) in EGFR-mutated lung adenocarcinoma. Five of the 19 miRNAs were previously listed as EGFR-mutation-specific miRNAs (i.e., miR-532-3p, miR-500a-3p, miR-224-5p, miR-502-3p, and miR-532-5p). An integrative analysis of miRNA and mRNA expression revealed a refined list of putative miRNA–mRNA interactions, of which 63 were potentially involved in EGFR-mutated tumors. Network structural analysis provided a comprehensive view of the complex miRNA–mRNA interactions in EGFR-mutated lung adenocarcinoma, including DUSP4 and MUC4 axes. Overall, this observational study provides insight into the unique miRNA–mRNA regulatory network present in EGFR-mutated tumors. Our findings, if validated, would inform future research examining the interplay of miRNAs and mRNAs in EGFR-mutated lung adenocarcinoma.

scholarly journals Desiccation tolerance in streptophyte algae and the algae to land plant transition: evolution of LEA and MIP protein families within the Viridiplantae

2020 ◽  
Vol 71 (11) ◽  
pp. 3270-3278 ◽  
Author(s):  
Burkhard Becker ◽  
Xuehuan Feng ◽  
Yanbin Yin ◽  
Andreas Holzinger
Keyword(s):  
Desiccation Tolerance ◽  
Experimental Studies ◽  
Sister Group ◽  
Land Plant ◽  
Late Embryogenesis Abundant ◽  
Land Plants ◽  
Desiccation Stress ◽  
Protein Families ◽  
Intrinsic Protein ◽  
Late Embryogenesis

Abstract The present review summarizes the effects of desiccation in streptophyte green algae, as numerous experimental studies have been performed over the past decade particularly in the early branching streptophyte Klebsormidium sp. and the late branching Zygnema circumcarinatum. The latter genus gives its name to the Zygenmatophyceae, the sister group to land plants. For both organisms, transcriptomic investigations of desiccation stress are available, and illustrate a high variability in the stress response depending on the conditions and the strains used. However, overall, the responses of both organisms to desiccation stress are very similar to that of land plants. We highlight the evolution of two highly regulated protein families, the late embryogenesis abundant (LEA) proteins and the major intrinsic protein (MIP) family. Chlorophytes and streptophytes encode LEA4 and LEA5, while LEA2 have so far only been found in streptophyte algae, indicating an evolutionary origin in this group. Within the MIP family, a high transcriptomic regulation of a tonoplast intrinsic protein (TIP) has been found for the first time outside the embryophytes in Z. circumcarinatum. The MIP family became more complex on the way to terrestrialization but simplified afterwards. These observations suggest a key role for water transport proteins in desiccation tolerance of streptophytes.

Sign in / Sign up

Export Citation Format

Share Document