scholarly journals Antioxidant and Antidiabetic Activities of Biologically Synthesized Silver Nanoparticles using Linumusitatissimum Extract

2021 ◽  
Vol 37 (5) ◽  
pp. Kanmani-R
Author(s):  
Kanmani R ◽  
IrudayaIrin Scleeva P
Keyword(s):  
Silver Nanoparticles ◽  
Research Work ◽  
Radical Scavenging ◽  
Xrd Analysis ◽  
Spectroscopic Studies ◽  
Uv Spectra ◽  
Alpha Amylase ◽  
Ir Spectroscopic ◽  
Flaxseed Extract

This research work was mainly focused to study the anti-oxidant and anti-diabetic activities of biologically synthesis of silver nanoparticles (AgNPs) from the flaxseed extract of Linumusitassimum. Qualitative tests identify the presence of phytochemicals in the flaxseed extract and its results showed the presence of tannins, terpenoids, saponins, flavonoids, steroids, cardiac glycosides, anthraquinones, coumarins, xanthoproteins, alkaloids, emodins, and carbohydrate in it. Preliminarily AgNPs formation is confirmed by the UV spectra and it showed maximum adsorption band at 438nm. FT-IR spectroscopic studies reveal the Phyto-constituents which are involved in the reduction of silver (Ag+1) into silver nanoparticles (Ag0). The spherical shapes of AgNPs are observed with crystalline nature are found in the aid of SEM and XRD analysis. Synthesized AgNPs have the maximum percentage of a silver element which is examined by the EDX analysis. The in-vitro antioxidant and antidiabetic activities of L. usitatissimummediated AgNPs were analyzed by using the DPPH, alpha-amylase, and alpha glycosides assays respectively. The DPPH result shows that the AgNPs possess 59.01% of radical scavenging property and the standard ascorbic acid reveals 48.63% at 100µg/ml concentration. Similarly in anti-diabetic activity, AgNPs shows the maximum inhibition of 79.84% in the alpha-amylase assay, and for alpha-glucosidase, AgNPs showed 58.86% at 100µg/ml concentration.

scholarly journals Multifaceted Phytogenic Silver Nanoparticles by a Rare Insectivorous Plant Drosera Spatulata Labill Var.bakoensis and its Enhanced Antioxidant, Antibacterial, Antifungal and in Vitro Cytotoxic Studies

2021 ◽  
Author(s):  
Susmila Aparna Gaddam ◽  
Venkata Subbaiah Kotakadi ◽  
Gunasekhar. Kalavakunta ◽  
Josthna Penchalaneni ◽  
Varadarajulu Naidu Challagundla ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Radical Scavenging ◽  
Human Colon ◽  
Xrd Analysis ◽  
Antimicrobial Activities ◽  
Human Colon Cancer ◽  
Tem Analysis ◽  
Insectivorous Plant ◽  
Average Size

Abstract The current investigation highlights the green synthesis of silver nanoparticles (AgNPs) by the insectivorous plant Drosera spatulata Labill var.bakoensis, which is the first of its kind. The biosynthesized nanoparticles revealed a UV visible surface plasmon resonance (SPR) band at 427 nm. The natural phytoconstituents which reduce the monovalent silver were identified by FTIR. The particle size of the Ds-AgNPs was detected by the Nanoparticle size analyzer confirms that the average size of nanoparticles was around 23 ± 2 nm. Ds-AgNPs exhibit high stability because of their high negative zeta potential (-34.1 mV). AFM studies also revealed that the Ds-AgNPs were spherical in shape and average size ranges from 10 to 20 ± 5 nm. TEM analysis also revealed that the average size of Ds-AgNPs was also around 21 ± 4 nm and the shape is roughly spherical and well dispersed. The crystal nature of Ds-AgNPs was detected as a face-centered cube by the XRD analysis. Furthermore, studies on antibacterial and antifungal activities manifested outstanding antimicrobial activities of Ds-AgNPs compared with standard antibiotic Amoxyclav. In addition, demonstration of superior free radical scavenging efficacy coupled with potential in vitro cytotoxic significance on Human colon cancer cell lines (HT-29) suggests that the Ds-AgNPs attain excellent multifunctional therapeutic applications.

scholarly journals Anti-Urolithiatic Activity of Silver Nanoparticles of Terminalia Chebula Bark

2021 ◽  
Vol 37 (1) ◽  
pp. 109-115
Author(s):  
S. Vidhya ◽  
A. Leema Rose ◽  
F. Janeeta Priya ◽  
T. Keerthana ◽  
R. Priyadharshini
Keyword(s):  
Silver Nanoparticles ◽  
Xrd Analysis ◽  
Spectroscopic Studies ◽  
Modern Medicine ◽  
Diffusion Method ◽  
Terminalia Chebula ◽  
Ft Ir ◽  
Crystalline Nature ◽  
Ir Spectroscopic ◽  
Gel Diffusion

The potential inhibitory action of herbals plays an indispensable role in modern medicine. The struvite crystal is an infectious stone related to urinary tract infection, hence the present study highlighted the anti-urolithiatic potential of Terminalia chebula bark mediated silver nanoparticles. Silver nanoparticles formation was confirmed by SPR band at 437nm. FT-IR spectroscopic studies suggested that phytoconstituents are primarily responsible for the reduction and capping of silver nanoparticles. TEM and XRD analysis revealed that the uniformity in shape and crystalline nature of T. chebula mediated silver nanoparticles. The single gel diffusion method was adopted to found the inhibition action of T.chebula mediated silver nanoparticles in struvite crystals. The inhibition efficiency was found to be 87.9 %, indicated that T. chebula mediated silver nanoparticles was a good inhibitor for the struvite crystals. The shift in band values of FTIR spectra confirms that the phyto constituents present in T. chebula bark is responsible for the anti-urolithiatic activity.

PHYTO-MEDIATED SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF „BUGLOSSOIDES PURPUROCAERULEA“ (BORAGINACEAE) AND THEIR BIOACTIVITY

2021 ◽  
Author(s):  
Jelena S. Katanić Stanković ◽  
◽  
Nikola Srećković ◽  
Vladimir Mihailović
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Bacterial Strains ◽  
Penicillium Species ◽  
Microdilution Method ◽  
Biological Potential ◽  
Vis Spectroscopy

In this study, silver nanoparticles (AgNPs) have been synthesized using the aqueous extract of the aerial parts of B. purpurocaerulea, collected in Serbia. B. purpurocaerulea silver nanoparticles (Bp– AgNPs) synthesis was confirmed using UV-Vis spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The biological potential of synthesized Bp-AgNPs was evaluated in vitro using ABTS assay for determining free radical scavenging potential and microdilution method for analysis of antimicrobial properties. Bp-AgNPs showed high antioxidant activity similar to Bp-extract, comparable to BHT. The synthesized nanoparticles exerted remarkable antibacterial effects, with minimal inhibitory concentration (MIC) values below 20 µg/mL. In the case of some bacterial strains, the results of Bp– AgNPs were comparable or similar to standard antibiotic erythromycin. The antifungal activity of Bp– AgNPs was moderate for most of the used strains. Nevertheless, several fungi were resistant to the NPs action, while two tested Penicillium species were extremely sensitive on Bp-AgNPs with MIC lower than 40 µg/mL. The antimicrobial properties of Bp-AgNPs can be useful for the development of new NPs-containing products.

Functionalized silver nanoparticles synthesized from marine algae: In vitro Evaluation of Antibacterial Potential

2021 ◽  
Vol 16 (8) ◽  
pp. 38-49
Author(s):  
Siva Kumar Kandula ◽  
Satyanarayana Swamy Cheekatla ◽  
Venkata Satya Mahesh Kumar Metta ◽  
Venkata Rajagopal Saladi
Keyword(s):  
Silver Nanoparticles ◽  
Antioxidant Activities ◽  
Algal Species ◽  
Synthesis Method ◽  
Radical Scavenging ◽  
Visible Spectrum ◽  
Total Phenolic ◽  
Face Centered Cubic ◽  
Frap Assay

Natural antioxidants, in particular phenolic derivatives, are used efficiently to combat against oxidative induced tissue damages. The objective of the study is to determine the antioxidant potential of methanolic extracts obtained from eight marine algal species (Enteromorpha compressa, Chaetomorpha antennina, Caulerpa racemosa, Caulerpa taxifolia, Sargassum vulgare, Padina tetrastromatica, Amphiroa fragilissima and Gracilaria corticata) by assessing their total phenolic content, DPPH scavenging assay, FRAP assay, H2O2 radical and superoxide radical scavenging activities. Among them, P.tetrastromatica, S.vulgare, E.compressa, C.taxifolia display significant antioxidant activities. Further, the aqueous extracts of these four algae are used for bioreduction of silver nitrate to silver nanoparticles (AgNPs) by green synthesis method at room temperature. UV-Visible spectrum revealed the surface plasmon resonance at 430 and 440nm. The characterizations of AgNPs by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) studies revealed the bioreduction and capping of AgNPs. XRD analysis elucidated the synthesized nanoparticles having face centered cubic crystalline geometry, with a mean size of 17 nm. The nanoparticles have better antimicrobial activity against cocci shaped than the rod shaped bacteria. The minimum inhibitory concentration and minimum bactericidal concentration exhibit more activity against S.aureus and B.cereus rather than E.coli.

scholarly journals In Vitro Antioxidant and Anti-Diabetic Potential of Gymnema Sylvestre Methanol Leaf Extract

2017 ◽  
Vol 13 (36) ◽  
pp. 218 ◽  
Author(s):  
Ibrahim A. ◽  
Babandi A. ◽  
Tijjani A.A. ◽  
Murtala Y. ◽  
Yakasai H.M. ◽  
...  
Keyword(s):  
Normal Control ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Significant Inhibition ◽  
Alpha Amylase ◽  
Gymnema Sylvestre ◽  
Total Flavonoids Content

Some medicinal plants and their purified derivatives have demonstrated beneficial therapeutic potentials for many centuries. They have been reported to exhibit antioxidant activity, reducing the oxidative stress in cells and are therefore useful in the treatment of many human diseases, including diabetes and other non-communicable diseases. This study evaluated antioxidative activity and enzymatic (alpha-amylase and alphaglucosidase) inhibitory potentials of Gymnema sylvestre methanolic leaf extract (GSMLE) using standard methods. Phytochemical screening revealed the presence of alkaloids, tannins, saponins, steroids, terpenoids and flavonoids. The total phenolics and total flavonoids content in the extract were found to be 6.629±0.745 (µg/ml of catechol equivalent) and 0.004±0.0012 (µg/ml of quercetin equivalent) respectively. GSMLE was shown to have radical scavenging activity against DPPH (290.54 ± 39.72 %), hydroxyl radical (86.507 ± 23.55 %) and hydrogen peroxide (45.25 ± 25.23 %). The level of SOD was significantly decreased in H2O2 induced and H2O2+extract induced when compared with normal control (p<0.05); the level of GSH was significantly increased in H2O2 induced control and significantly decreased in H2O2+extract induced test when compared to normal control. GSH was also decreased significantly in H2O2+extract induced when compared to H2O2 induced control (p<0.05). The extract also demonstrated significant inhibition of alpha-glucosidase (IC50 182.26 ±1.05μg/ml) when compared with standard acarbose (IC50 189.52±0.46) and was more potent than the arcarbose on alpha-amylase inhibition with IC50 of 195.3±4.40 and 200.05±7.16 respectively. These findings may therefore, stress the potentiality of using Gymnema sylvestre as a natural remedy for the management of type 2 diabetes.

scholarly journals Assessment of in-vitro Antioxidant/Enzymes Inhibitory Potentials of Aframomum melegueta [Roscoe] K. Schum (Grains of Paradise) Leaf, Stem Bark, Seed Bark and Seed Extracts

2020 ◽  
pp. 40-57
Author(s):  
Oludare Temitope Osuntokun ◽  
V. O. Olumekun ◽  
A. O. Ajayi ◽  
I. O. Omotuyi ◽  
A. Olonisakin
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Free Radical ◽  
Reduced Glutathione ◽  
Research Work ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Oh Radical ◽  
Seed Extracts

The purpose of this research work is to evaluates the in-vitro antioxidant and antioxidant enzymes inhibition potentials of Aframomum melegueta [Roscoe] K. Schum (Grains of Paradise) Leaf, Stem Bark, Seed Bark and Seed Extracts. Aframomum melegueta is a spice with a similar composition as Ginger, that belongs to the same Zingiberaceae family, used in Nigeria, West Africa and it is used for the treatment of infectious diseases such as urinary tract infections, cancer and diabetes. Antioxidants neutralize the effect of free radicals through different ways and may prevent the body from various diseases. The plant extract were collected from a rain forest in Akugba Akoko, Ondo state, Nigeria. The plant sample was dried, pulverized, filtered with Whatman No 1 filtered paper and the filtrates was concentrated in-vacuum using vacuum rotary evaporator at 40°C and was later concentrated to dryness in a hot-air oven at 40°C. Assessment of antioxidant potentials were performed using Singleton method Total flavonoids (mg/g), Phenol (mg/g), Ferric reducing (FRAP) potentials (mg/g), Free radical scavenging ability (DPPH) (1, 1- diphenyl-2-picryhydrazyl) potentials (%),2,2’-azino-bis(3-ethylbenthiazoline-6-sulphonic acid), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging potentials (mMol/g), Fe2+ chelation potentials (%) and OH Radical Scavenging potentials (%) were evaluated. Antioxidant enzymes of plant were assessed using Bentller method, Superoxide Dismutase (SOD) (%), Reduced Glutathione (GSH) (%), Catalase (CAT) and Glutathione Peroxidise (GPX) were evaluated. Seed extract among other extracts of Aframomum melegueta has the highest quantity of flavonoids, Phenol, FRAP, DPPH, ABTS, Fe2+ chelation and OH radical scavenging potential. The leaf extract of A. meleguata has the highest percentage of Superoxide Dismutase (SOD) and Catalase (CAT) while stem bark of Aframomum melegueta has the highest percentage of Reduced Glutathione (GSH) and Glutathione Peroxidise (GPX).it can be deduced from this research work that all parts of the A. melegueta has pharmacological and therapeutic activities judging from literature that A. melegueta can be used to prevent oxidative damage by ROS (Reactive oxygen species) reacting with free radical chelating and catalytic metals  which can be used to reduce and prevent various diseases like heart disease, cancer, DNA degeneration, pulmonary and neurological disorder. SOD, GPX, GSH help to control cytokins induced peroxidise level and mediate signal transduction in mammalian cells. It can be deduced that Aframomum melegueta should be the focal point in human diet, natural herbal drug supplement and even in addition to conventional drug to improve the potency and quality of natural drug system. The use of Aframomum melegueta should be encouraged. These results show the potential of Aframomum melegueta as a source of bioactive compounds to be used for pharmaceutical, nutraceutical, and cosmeceutical applications.

scholarly journals Bio-Inspired Fabrication of Silver Nanoparticles Using High Altitude Squamulose Lichen Extract and Evaluation of its Antioxidant, Anticandida and Cytotoxic properties

2021 ◽  
Author(s):  
S Shanthi ◽  
V. Uma Maheshwari Nallal ◽  
Krishnan Anand ◽  
Balasubramani Ravindran ◽  
Soon Woong Chang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Silver Nanoparticles ◽  
High Altitude ◽  
Usnic Acid ◽  
Nanoparticle Synthesis ◽  
Spectroscopic Studies ◽  
Binding Energies ◽  
Average Size ◽  
Nano Medicine

Abstract Bio-inspired nanoparticle synthesis has attracted substantial interest among the scientific society owing to its eco-friendly and non-toxic nature. In the present study, Silver nanoparticles (AgNPs) were synthesized using high altitude squamulose lichen – Cladonia subradiata and characterized using different techniques. The antioxidant and anticandida activity of AgNPs were evaluated using multiple in-vitro assays. In-silico molecular docking analysis and in-vitro cytotoxic assay was performed to determine the anti-cancer potential of synthesized AgNPs. The results of the spectroscopic studies revealed the successful synthesis of AgNPs and the presence of different functional groups suggesting the involvement of phytocompounds in the reduction and capping of AgNPs. The average size of the AgNPs was 20 nm and predominantly spherical in shape. AgNPs demonstrated excellent DPPH free radicals scavenging activity with an IC50 value of 7.51 ± 0.4 µg/mL. C.albicans was identified as the most susceptible strain from the anticandida studies. Usnic acid and Pulvinic acid exhibited low binding energies and showed excellent inhibition interaction with EGFR lung cancer protein. The in-vitro cytotoxic results were impressive with an IC50 value of 28.75 µg/mL for A549 lung cancer cells treated with AgNPs. Thus, the study demonstrates the effective and non-toxic synthesis of AgNPs using a less explored lichen extract as a promising anticandida and anticancer agent in the field of nano-medicine.

scholarly journals Antioxidant and Hepatoprotective Potentials of Lasimorpha senegalensis Schott Leaf Extract on Carbon Tetrachloride-induced Liver Damage in Rats

2020 ◽  
pp. 70-78
Author(s):  
Chinyere Blessing Chigor ◽  
Felix Ifeanyi Nwafor ◽  
Edith Ugwuja ◽  
Chisimdi S. Obi
Keyword(s):  
Liver Damage ◽  
Leaf Extract ◽  
Liver Enzymes ◽  
Radical Scavenging Activity ◽  
Research Work ◽  
Radical Scavenging ◽  
Albino Rats ◽  
Endogenous Antioxidants ◽  
Wistar Albino Rats

Aims: The present study assessed the antioxidant and hepatoprotective potentials of the methanolic leaf extract of Lasimorpha senegalensis – a medicinal plant used by the indigenous people of Nigeria to treat hepatitis and feverish conditions. Place and Duration of Study: The research work was conducted in the Department of Pharmacognosy and Environmental Medicine and Department of Plant Science and Biotechnology, both in the University of Nigeria, Nsukka, from May to August, 2019. Methodology: Phytochemical analyses and acute toxicity study of the sample followed standard procedures. In vitro antioxidant assay was by DPPH and H2O2 models. A total of 25 male Wistar albino rats (120 – 150 g) were grouped into five, each group with five animals. Hepatotoxicity was induced with carbontetrachloride (1 ml/kg). The treatment groups (3-5) received extract (200 and 400 mg/kg) and Silymarin (100 mg/kg). Endogenous antioxidants (superoxide dismutase, catalase, glutathione peroxidase), plasma malondialdehyde and liver enzymes (aspartate aminotransferase, alanine aminotransferase, alkaline phosphate) were determined after treatment. Results: The results showed the leaf extract had appreciable amounts of bioactive phytochemicals and free radical scavenging activity (IC50 of 0.52 mg/ml and 0.71 mg/ml for DPPH and H2O2 respectively) with no toxicity at 5000 mg/kg. The extract also elevated the endogenous antioxidants and significantly (p ≤ .05) reduced lipid peroxidase and liver enzymes. Conclusion: This report justifies the local use of this plant in the management of various diseases related to oxidative stress and liver damage.

scholarly journals Synthesis of substituted pyridine based sulphonamides as an antidiabetic agent

2021 ◽  
Vol 12 (3) ◽  
pp. 279-283
Author(s):  
Gautam Sadawarte ◽  
Samadhan Jagatap ◽  
Mukesh Patil ◽  
Vasant Jagrut ◽  
Jamatsing Darbarsing Rajput
Keyword(s):  
Heterocyclic Compounds ◽  
Biological Activities ◽  
Research Work ◽  
Synthetic Methods ◽  
Alpha Amylase ◽  
Spectroscopic Techniques ◽  
Inhibition Activity ◽  
Antidiabetic Agent ◽  
Biological Functions ◽  
Ir Spectroscopic

This research work describes the synthesis of a new series of heterocyclic compounds, namely sulfonamide derivatives. Sulfonamides are a diverse class of organic compounds having significant and potent biological activities. Diverse synthetic methods have been engaged to build up its various derivatives for different biological functions. In this study, the production of novel pyridine-based heterocyclic compounds having sulfonamide moieties has been elaborated. The obtained sulfonamide-based pyridine scaffold was used to investigate their alpha-amylase inhibition activity. The structures of freshly prepared compounds were described using 1H NMR, 13C NMR, and IR spectroscopic techniques. The molecular docking of sulfonamides performed against porcine pancreatic alpha-amylase using PDB file 1LP was used for generation of grid. All the new synthesized compounds were shown notable anti-diabetic activity.

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