scholarly journals Bio-Inspired Fabrication of Silver Nanoparticles Using High Altitude Squamulose Lichen Extract and Evaluation of its Antioxidant, Anticandida and Cytotoxic properties

2021 ◽  
Author(s):  
S Shanthi ◽  
V. Uma Maheshwari Nallal ◽  
Krishnan Anand ◽  
Balasubramani Ravindran ◽  
Soon Woong Chang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Silver Nanoparticles ◽  
High Altitude ◽  
Usnic Acid ◽  
Nanoparticle Synthesis ◽  
Spectroscopic Studies ◽  
Binding Energies ◽  
Average Size ◽  
Nano Medicine

Abstract Bio-inspired nanoparticle synthesis has attracted substantial interest among the scientific society owing to its eco-friendly and non-toxic nature. In the present study, Silver nanoparticles (AgNPs) were synthesized using high altitude squamulose lichen – Cladonia subradiata and characterized using different techniques. The antioxidant and anticandida activity of AgNPs were evaluated using multiple in-vitro assays. In-silico molecular docking analysis and in-vitro cytotoxic assay was performed to determine the anti-cancer potential of synthesized AgNPs. The results of the spectroscopic studies revealed the successful synthesis of AgNPs and the presence of different functional groups suggesting the involvement of phytocompounds in the reduction and capping of AgNPs. The average size of the AgNPs was 20 nm and predominantly spherical in shape. AgNPs demonstrated excellent DPPH free radicals scavenging activity with an IC50 value of 7.51 ± 0.4 µg/mL. C.albicans was identified as the most susceptible strain from the anticandida studies. Usnic acid and Pulvinic acid exhibited low binding energies and showed excellent inhibition interaction with EGFR lung cancer protein. The in-vitro cytotoxic results were impressive with an IC50 value of 28.75 µg/mL for A549 lung cancer cells treated with AgNPs. Thus, the study demonstrates the effective and non-toxic synthesis of AgNPs using a less explored lichen extract as a promising anticandida and anticancer agent in the field of nano-medicine.

scholarly journals Enhanced antibacterial activity through silver nanoparticles deposited onto carboxylated graphene oxide surface

2021 ◽  
Author(s):  
Arturo Barjola ◽  
María Ángeles Tormo ◽  
Oscar Sahuquillo ◽  
Patricia Bernabé ◽  
José Manuel Pérez ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Biofilm Formation ◽  
Material Surface ◽  
Oxide Surface ◽  
Main Role ◽  
Carboxylic Groups ◽  
Average Size

Abstract The strong bactericidal action of silver nanoparticles (AgNPs) is usually limited for their degree of aggregation. Deposition of AgNPs onto a graphene oxide (GO) surface to generate GO-Ag hybrids has been shown to be an effective method to control these aggregation problems. In this sense, a novel carboxylated graphene oxide-silver nanoparticle (GOCOOH-Ag) material has been synthesized and their antibacterial and biofilm formation inhibition have been studied.AgNPs decorating the GOCOOH surface achieved an average size of 6.74±0.25 nm, which was smaller than those of AgNPs deposited onto the GO surface. In addition, better distribution of AgNPs was obtained using carboxylated material. It is important to highlight the main role of the carboxylic groups in the nucleation and growth of the AgNPs that decorate the GO-based material surface.In vitro antibacterial activity and antibiofilm-forming action were tested against Gram-positive (Staphylococcus aureus and Staphylococcus epidermidis) and Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). Both GO-Ag and GOCOOH-Ag reduced the bacterial growth, analyzed by time-kill curves. However, the minimum inhibitory concentration and the minimum bactericidal concentration of GOCOOH-Ag were lower than those of GO-Ag for all strains studied, indicating that GOCOOH-Ag has better antibacterial activity. In addition, both nanomaterials prevent biofilm-formation, with a higher reduction of biofilm mass and cell viability in the presence of GOCOOH-Ag. The carboxylation functionalization in GO-based materials can be applied to improve the bactericidal and antibiofilm-forming action of the AgNPs.

scholarly journals Synthesis Of PEG Coated Ascorbic Acid Functionalized Silver Nanoparticles and Determining its Antioxidant Activity

2019 ◽  
Vol 8 (4S2) ◽  
pp. 741-744
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Ascorbic Acid ◽  
Nanoparticle Synthesis ◽  
Drug Carriers ◽  
Functionalized Nanoparticles ◽  
Antioxidative Effects ◽  
Uv Visible ◽  
Average Size ◽  
Scanning Electron

Silver nanoparticles are extensively being studied for various applications in different fields. Functionalized silver nanoparticles are now being used as drug carriers. Ascorbic acid (Vitamin C) is a well-known antioxidant which has been reported to have various beneficial properties to humans. Here we have synthesized silver nanoparticles functionalized with ascorbic acid. The functionalized nanoparticles were further coated with PEG. The nanoparticle synthesis was characterized using UV-Visible spectral analysis, XRD, FTIR and Scanning electron microscope. The average size of the nanoparticle was found to be 24 nm. The synthesized nanoparticle was found to possess antioxidative effects.

scholarly journals Multifaceted Phytogenic Silver Nanoparticles by a Rare Insectivorous Plant Drosera Spatulata Labill Var.bakoensis and its Enhanced Antioxidant, Antibacterial, Antifungal and in Vitro Cytotoxic Studies

2021 ◽  
Author(s):  
Susmila Aparna Gaddam ◽  
Venkata Subbaiah Kotakadi ◽  
Gunasekhar. Kalavakunta ◽  
Josthna Penchalaneni ◽  
Varadarajulu Naidu Challagundla ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Radical Scavenging ◽  
Human Colon ◽  
Xrd Analysis ◽  
Antimicrobial Activities ◽  
Human Colon Cancer ◽  
Tem Analysis ◽  
Insectivorous Plant ◽  
Average Size

Abstract The current investigation highlights the green synthesis of silver nanoparticles (AgNPs) by the insectivorous plant Drosera spatulata Labill var.bakoensis, which is the first of its kind. The biosynthesized nanoparticles revealed a UV visible surface plasmon resonance (SPR) band at 427 nm. The natural phytoconstituents which reduce the monovalent silver were identified by FTIR. The particle size of the Ds-AgNPs was detected by the Nanoparticle size analyzer confirms that the average size of nanoparticles was around 23 ± 2 nm. Ds-AgNPs exhibit high stability because of their high negative zeta potential (-34.1 mV). AFM studies also revealed that the Ds-AgNPs were spherical in shape and average size ranges from 10 to 20 ± 5 nm. TEM analysis also revealed that the average size of Ds-AgNPs was also around 21 ± 4 nm and the shape is roughly spherical and well dispersed. The crystal nature of Ds-AgNPs was detected as a face-centered cube by the XRD analysis. Furthermore, studies on antibacterial and antifungal activities manifested outstanding antimicrobial activities of Ds-AgNPs compared with standard antibiotic Amoxyclav. In addition, demonstration of superior free radical scavenging efficacy coupled with potential in vitro cytotoxic significance on Human colon cancer cell lines (HT-29) suggests that the Ds-AgNPs attain excellent multifunctional therapeutic applications.

In Vivo Effect of Different Doses of Silver Nanoparticles on the Seminiferous Tubules in Albino Rats: Histopathological Study

2020 ◽  
Vol 38 (1B) ◽  
pp. 1-5
Author(s):  
Ruqayah A. Salman ◽  
Abdulrahman K. Ali ◽  
Amenah Ali Salman
Keyword(s):  
Silver Nanoparticles ◽  
Harmful Effect ◽  
Seminiferous Tubules ◽  
Albino Rats ◽  
Histopathological Study ◽  
Ag Nps ◽  
Average Size ◽  
Different Doses

The study aims to investigate the effects of silver nanoparticles (Ag NPs) on the seminiferous tubules in Albino rats. Several in vitro studies have been performed in different cell models, using various nanoparticles. Pure and spherical AgNPs with an average size of 30 nm, was injected into two groups of male albino rats (6 rats for each group) in different doses. Histopathological changes in testis tissues were showed a harmful effect of the silver nanoparticles, manifested by reducing the number of spermatogenic cells, and a decrease in the number of leyidg´s cells (group 1), and hypotrophy in seminiferous and enlargement in interstitial spaces in group 2.

scholarly journals Synergistic Antifungal Activity of Green Synthesized Silver Nanoparticles and Epoxiconazole against Setosphaeria turcica

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Weidong Huang ◽  
Minhui Yan ◽  
Haiming Duan ◽  
Yaling Bi ◽  
Xinxin Cheng ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antimicrobial Agents ◽  
Integrated Control ◽  
Antimicrobial Effect ◽  
Colony Growth ◽  
Setosphaeria Turcica ◽  
Ligustrum Lucidum ◽  
In Vitro Inoculation ◽  
Average Size

It is urgent to develop highly efficient and eco-friendly antimicrobial agents for integrated control of phytopathogens. Silver nanoparticles (AgNPs) were synthesized by Ligustrum lucidum leaf extract. UV-vis spectrum showed that there was a strong absorbance at 438 nm. Transmission electron microscopy (TEM) images displayed that synthesized nanoparticles were near spherical with an average size of 13 nm. The antimicrobial effect of AgNPs was evaluated through methods of paper disk diffusion, colony growth, conidia germination, and in vitro inoculation. The 50% inhibition concentration (IC50) of AgNPs against Setosphaeria turcica was 170.20 μg/mL calculated by SPSS 13.0. In addition, it displayed a significant synergistic antifungal effect when AgNPs were combined with epoxiconazole at the ratios of 8 : 2 and 9 : 1. The results of this study provide a novel fungistat not only for comprehensive control of plant fungi but also for reducing chemical pesticides use and avoiding drug-resistant phytopathogen generation.

scholarly journals Biosynthesis of Silver Nanoparticles Using Ligustrum Ovalifolium Fruits and Their Cytotoxic Effects

Nanomaterials ◽  
2018 ◽  
Vol 8 (8) ◽  
pp. 627 ◽  
Author(s):  
Bianca Moldovan ◽  
Vladislav Sincari ◽  
Maria Perde-Schrepler ◽  
Luminita David
Keyword(s):  
Silver Nanoparticles ◽  
Synthesis Method ◽  
Cost Effective ◽  
Bioactive Molecules ◽  
Energetic Cost ◽  
Fruit Extract ◽  
Vis Spectroscopy ◽  
Biosynthesized Agnps ◽  
Average Size

The present study reports for the first time the efficacy of bioactive compounds from Ligustrum ovalifolium L. fruit extract as reducing and capping agents of silver nanoparticles (AgNPs), developing a green, zero energetic, cost effective and simple synthesis method of AgNPs. The obtained nanoparticles were characterized by UV-Vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier Transform Infrared spectroscopy (FTIR), confirming that nanoparticles were crystalline in nature, spherical in shape, with an average size of 7 nm. The FTIR spectroscopy analysis demonstrated that the AgNPs were capped and stabilized by bioactive molecules from the fruit extract. The cytotoxicity of the biosynthesized AgNPs was in vitro evaluated against ovarian carcinoma cells and there were found to be effective at low concentration levels.

scholarly journals Surface and Structural Properties of Medical Acrylonitrile Butadiene Styrene Modified with Silver Nanoparticles

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 197 ◽  
Author(s):  
Magdalena Ziąbka ◽  
Michał Dziadek ◽  
Kinga Pielichowska
Keyword(s):  
Silver Nanoparticles ◽  
Water Solution ◽  
Young Modulus ◽  
Acrylonitrile Butadiene Styrene ◽  
Spectroscopic Studies ◽  
Homogeneous Distribution ◽  
The Stability ◽  
Butadiene Styrene

Acrylonitrile butadiene styrene/silver nanoparticles (ABS/AgNPs) composites were manufactured through the plastic processing method. Three different matrices were used to obtain polymer and composite samples containing 0.5 wt % and 1.0 wt % of silver nanoparticles, respectively. The aim of this study was to examine physicochemical properties and stability of the materials in the in vitro conditions for two years. The results showed that composites made from amorphous matrices had comparable mechanical properties after incorporation of AgNPs. The values of Young modulus and tensile strength increased after the first and second year of investigation. Silver nanoparticles did not alter the surface parameters—e.g., roughness and contact angle also retained stable values after the in vitro incubation in water solution. The scanning electron observation revealed homogeneous distribution of silver modifier in all the matrices. The 24-month incubation of materials proved the stability of the composites microstructure. The DSC analysis revealed that addition of AgNPs may decrease glass transition temperature of the composite materials which was also reduced after 12 and 24 months of incubation. The attenuated total reflectance–Fourier transform infrared (ATR-FTIR) spectroscopic studies did not indicate significant changes in the ABS matrices either upon their modification with AgNPs or after the long-term testing. The conducted studies proved that all the composites are stable and may be used for a long-term working period.

scholarly journals Investigation of In Vitro Antioxidant and Antibacterial Potential of Silver Nanoparticles Obtained by Biosynthesis Using Beech Bark Extract

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 459 ◽  
Author(s):  
Tanase ◽  
Berta ◽  
Coman ◽  
Roșca ◽  
Man ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Metal Ion ◽  
Color Change ◽  
Vital Role ◽  
Bark Extract ◽  
Ft Ir ◽  
Antioxidant And Antimicrobial Activity ◽  
Average Size

Green synthesis is one of the rapid and best ways for silver nanoparticles (AgNP) synthesis. In the present study, synthesis and bioactivity of AgNPs has been demonstrated using water beech (Fagus sylvatica L.) bark extract. The physical and chemical factors such as time, metal ion solution, and pH, which play a vital role in the AgNPs synthesis, were assessed. The AgNPs were characterized by ultraviolet-visible (UV-Vis) spectrometry, Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM). Antioxidant and antimicrobial activity of the obtained AgNPs was evaluated. AgNPs were characterized by color change pattern, and the broad peak obtained at 420–475 nm with UV-Vis confirmed the synthesis of AgNPs. FT-IR results confirmed that phenols and proteins of beech bark extract are mainly responsible for capping and stabilization of synthesized AgNPs. TEM micrographs showed spherical or rarely polygonal and triangular particles with an average size of 32 nm at pH = 9, and 62 nm at pH = 4. Furthermore, synthesized AgNPs were found to exhibit antioxidant activity and have antibacterial effect against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa. These results indicate that bark extract of F. sylvatica L. is suitable for synthesizing stable AgNPs, which act as an excellent antimicrobial agent.

scholarly journals Development of Functionalized silver Nanoparticles from Allamanda neriifolia Hook and their In Vitro Cytotoxic Effect on MCF-7 Cells

YMER Digital ◽  
2021 ◽  
Vol 20 (12) ◽  
pp. 385-396
Author(s):  
Sumathi R ◽  
◽  
Sivagamasundari K ◽  
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Morphological Changes ◽  
Total Phenolic ◽  
Biosynthesized Agnps ◽  
Average Size ◽  
Mcf 7

The present work demonstrates the influence of plant extract composition (antioxidant and total phenolic content) on the size and morphology of the produced AgNPs. In this study, silver nanoparticles (AgNPs) were synthesized using aqueous flower extract of Allamanda neriifolia plant. The biosynthetic procedure was rapid and simple and was easily monitored via colour changes and examined AgNPs (AN-AgNPs) by ultraviolet-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM). The results obtained from various characterizations revealed that average size of synthesized AgNPs was 50 nm and in spherical structure. The anticancer potential of AN-AgNPs was investigated against human breast cancer cells (MCF-7). The cytotoxic response was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), and morphological changes by apoptosis. The biosynthesized AgNPs-induced cell death in MCF- 7 cells suggested the anticancer potential of AN-AgNPs. Therefore, they may be used to treat the breast cancer cells.

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