scholarly journals EFEK ANTI HISTAMIN EKSTRAK DAUN JAMBU BIJI PADA TIKUS PUTIH YANG DIINDUKSI OVALBUMIN DINILAI DARI JUMLAH EOSINOFIL JARINGAN KULIT

2020 ◽  
Vol 2 (2) ◽  
pp. 66
Author(s):  
RENDRA RISTIAN WIBOWO ◽  
RATIH DEWI YUDHANI ◽  
NOVAN ADI SETYAWAN
Keyword(s):  
Leaf Extract ◽  
Allergic Diseases ◽  
Psidium Guajava ◽  
Skin Tissue ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Quasi Experimental ◽  
Guava Leaf ◽  
Post Hoc

<p class="Default"><strong>                                                     ABSTRAK</strong></p><p class="Default"><strong>Pendahuluan:</strong> Prevalensi dan keparahan penyakit alergi terus meningkat secara global, terutama di negara dengan pendapatan rendah sampai menengah. Terapi alergi saat ini seperti kortikosteroid banyak memberikan efek samping dalam penggunaan jangka panjang. Ekstrak daun jambu biji (Psidium guajava) berpotensi sebagai antihistamin yang dapat menjadi terapi alternatif penyakit alergi. Penelitian ini bertujuan untuk mengetahui efek antihistamin ekstrak daun jambu biji (Psidium guajava) pada tikus putih (Rattus novergicus) yang diinduksi ovalbumin dinilai dari jumlah eosinofil jaringan kulit.   </p><p class="Default"><strong>Metode: </strong>Penelitian ini merupakan penelitian <em>quasi experimental design</em> dengan rancangan <em>post test only with control group design. </em>Subjek penelitian tikus putih jantan<em>, </em>umur 3-4 bulan, dan berat badan 200-300 gram. Sampel 24 ekor tikus putih dibagi secara random menjadi enam kelompok. Kelompok kontrol normal (K) tanpa perlakuan, kontrol negatif (KN) hanya diinduksi ovalbumin, kontrol positif (KP) diberikan metilprednisolon, dan kelompok perlakuan (KP1, KP2, KP3) diberikan ekstrak daun jambu biji dengan dosis berikut 27 mg/200 gramBB, 54 mg/200 gramBB, dan 108 mg/200 gramBB. Efek antihistamin dinilai melalui gambaran histopatologi jaringan kulit berupa rata-rata jumlah infiltrasi eosinofil dengan menggunakan mikroskop cahaya. Data dianalisis menggunakan uji <em>Kruskal Wallis</em> dan dilanjutkan uji <em>Post Hoc Mann Whitney</em> (α= 0,05).</p><p class="Default"><strong>Hasil: </strong>Uji <em>Kruskal Wallis </em>menunjukkan perbedaan signifikan di antara enam kelompok perlakuan (p=0,00). Uji <em>Post Hoc Mann Whitney </em>menunjukkan perbedaan signifikan di antara semua pasangan kelompok (p=&lt;0,05). KP3 menunjukkan rerata jumlah eosinofil lebih rendah daripada KP dan signifikan (p=0,021). <sup> </sup></p><p class="Default"><strong>Kes</strong><strong>impulan: </strong>Ekstrak daun jambu biji (<em>Psidium guajava</em>) memiliki efek antihistamin dengan menurunkan infiltrasi eosinofil pada jaringan kulit tikus putih (<em>Rattus norvegicus</em>) yang dinduksi ovalbumin.</p><p><strong>Kata Kunci: </strong>Ekstrak daun jambu biji, eosinofil, tikus putih, histopatologi kulit.</p><p align="center"><strong> </strong><strong><em>ABSTRACT</em></strong></p><p class="Default"><strong><em>Introduction</em></strong><strong><em>: </em></strong><em>The prevalence and severity of allergic diseases continues to increase globally, especially in countries with low to middle income. Current allergic therapies such as corticosteroids have many side effects in long-term use. Guava leaf extract (Psidium guajava) has the potential as an antihistamine which can be an alternative therapy for allergic diseases. This study aims to determine the effect of antihistamine extract of guava leaves (Psidium guajava) on white rats (Rattus novergicus) induced by ovalbumin assessed from the number of eosinophils of skin tissue.</em><em></em></p><p class="Default"><em> </em></p><p class="Default"><strong><em>Methods</em></strong><em>: </em><em>This research was a quasi experimental design with a post test only design with control group design. The research subjects were male white rats, aged 3-4 months, and weighing 200-300 grams. Samples of 24 white rats were randomly divided into six groups. Normal (K) control group without treatment, negative control (KN) was only induced by ovalbumin, positive control (KP) was given methylprednisolone, and treatment groups (KP1, KP2, KP3) were given guava leaf extract with the following dosage 27 mg / 200 gram BW, 54 mg / 200 gram BW, and 108 mg / 200 gram BW. The effect of antihistamines was assessed through histopathology of skin tissue in the form of an average number of eosinophil infiltrations using a light microscope. Data were analyzed using the Kruskal Wallis test and followed by the Mann Whitney Post Hoc test (α = 0.05).</em><em></em></p><p class="Default"><strong><em>Result</em></strong><em>: </em><em>The Kruskal Wallis test showed significant differences between the six treatment groups (p = 0.00). The Mann Whitney Post Hoc test showed significant differences between all group pairs (p = &lt;0.05). KP3 shows the average number of eosinophils lower than KP and significant (p = 0.021).</em><em> </em><em></em></p><p><strong><em>Conclusion</em></strong><em>: Guava leaf extract (Psidium guajava) has an antihistamine effect by reducing eosinophil infiltration in ovalbumin-induced white rat (Rattus norvegicus) skin tissue.<strong></strong></em></p><p><strong><em>Keywords: </em></strong><strong><em>Guava leaf extract; eosinophil; skin histopathology</em></strong></p>

scholarly journals THE COMPARISON OF RAMANIA (Bouea macrophylla Griff) AND BINJAI (Mangifera caesia) LEAVES EXTRACT GEL EFFECT ON COLLAGEN DENSITY

2021 ◽  
Vol 6 (2) ◽  
pp. 159
Author(s):  
Nabilah Nabilah ◽  
Irham Taufiqurrahman ◽  
Nolista Indah Rasyid
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Total Sample ◽  
Control Group ◽  
Control Group Design ◽  
Gel Effect ◽  
Index Measurement ◽  
Male Wistar Rats ◽  
Collagen Density ◽  
Post Hoc

Background: The extract of ramania Bouea macrophylla Griff) and binjai (Mangifera caesia) leaf have flavonoid compounds that function as antioxidants to balance the amount of Reactive Oxygen Species (ROS) in tissues and optimize wound healing by helping synthesis of hydroxyproline which is used as a collagen synthesis material. Objective: To compare the effect of 15% concentration of ramania leaf extract gel, 15% concentration of binjai leaf extract gel on collagen density in back incision wounds of male Wistar rats on day 7 and day 14. Methods: This study used a true experimental design with a posttest-only with control group design. The study sample used male wistar rats that were healthy and active, aged 2-3 months with a body weight of 250-300 grams. The total sample was 18 rats divided into 6 groups. The 15% concentration of ramania and binjai leaves was given topically, then the rats were euthanized on the 7th and 14th day. Collagen index measurement was using hydroxyproline concentration. Results: Two-way Anova data analysis showed a significant value of 0.00 (p<0.05), which means that there was a difference in effect between the treatment gel and wound day. Bonferroni Post Hoc test showed a significant value in all treatment gel groups. Conclusion: There is a difference in the effect of 15% concentration of ramania leaf extract gel and 15% concentration of binjai extract gel on collagen density. Binjai leaf extract gel at 15% concentration is more effective for collagen density than Ramania leaf extract at 15% concentration and placebo. Keywords : Binjai Leaf Extract Gel, Collagen, Hydroxyproline, Ramania Leaf Extract Gel.

scholarly journals EFFECTIVENESS OF LEAF EXTRACT REDUCE INFLAMMATION REACTION IN HYPERCHOLESTEROLEMIA RATS

2017 ◽  
Vol 1 (1) ◽  
pp. 12
Author(s):  
Puguh Santoso ◽  
Ni Nyoman Wahyu Udayani ◽  
I Nyoman Gede Tri Sutrisna ◽  
Ketut Agus Adrianta
Keyword(s):  
Free Radicals ◽  
Leaf Extract ◽  
Oxidized Ldl ◽  
Oxidative Modification ◽  
Blood Cholesterol ◽  
Ldl Oxidation ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Group I

Abstract High blood cholesterol is often called hypercholesterolemia is a risk factor for the emergence of pathological conditions such as heart and blood vessel disease. Hypercholesterolemia has an important role in the occurrence of damage to the endothelial cells is mainly caused by oxidized LDL. Oxidation of LDL triggers the formation of TNF - ?. Leaves messengers that allegedly contains flavonoids can improve the situation of hypercholesterolemia through the barriers specifically the expression of TNF - ? increased due to hypercholesterolemia. Plants messengers known to contain alkaloids, flavonoids, tannins, saponins, polyphenols, calcium oxalate, fats, and essential oil. Flavonoids which has the ability to bind the atom to form free radicals not to excess free radicals, thereby inhibiting the oxidative modification of LDL become ox-LDL so it will not be formed atherosclerosis. This study uses the Randomize pattern Pre and Post Test Control Group Design, using white rats (Rattusnovergicus) with Wistar strain aged 3-4 months, weighing 175-200 grams. Divided into four groups: Group I as a control with placebo, Group II treatment using extracts of ethanol leaves a messenger at a concentration of 10%, Group III treatments using extracts of ethanol leaves a messenger at a concentration of 20%, Group IV is to use the extract ethanol leaves errand at a concentration of 30%. It can be concluded at 4 dose group 30% messengers leaf extract significantly different, p <0.05, so it can be said that the provision of effective messengers extract at a dose of 30%.

scholarly journals ANTIBACTERIAL EFFECTIVITY OF KASTURI LEAF EXTRACT (Mangifera casturi) AGAINST THE GROWTH OF Streptococcus sanguinis BACTERIA

2020 ◽  
Vol 5 (1) ◽  
pp. 33
Author(s):  
Dita Puspita Sari ◽  
Didit Aspriyanto ◽  
Irham Taufiqurrahman
Keyword(s):  
Leaf Extract ◽  
Periodontal Diseases ◽  
Tooth Surface ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Streptococcus Sanguinis ◽  
Significant Difference ◽  
Post Hoc ◽  
One Way Anova

Background: Caries is a disease that occurs because of the fermentation carbohydrates process by microorganisms in the oral cavity. One of the bacteria that causes caries is Streptococcus sanguinis. These bacteria will colonize on the tooth surface, then form dental plaques and contribute to the causes of caries and other periodontal diseases. Kasturi leaf extract (Mangifera casturi) has various compounds such as tannins, terpenoids, alkaloids, and flavonoids that have antimicrobial substances. Purpose: The aim of this study was to determine antibacterial effectivity of kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis bacteria. Method: This research was an experimental method laboratory (true experimental), with a randomized pre test and post test with control group design using 5 treatments: kasturi leaf extract (concentration: 20 mg/ml, 25 mg/ml, and 30 mg/ml); and two groups of control: positive control and negative control. Each treatment was repeated 5 times. Antibacterial activity testing used a liquid dilution method. Measurement of minimum inhibitory concentration (MIC) used a Uv-Vis Spectrophotometer and measurement of the minimum bactericidal concentration (MBC) used a colony counter. The MIC data were analyzed using One Way Anova and continued with the Dunnet Post Hoc test. MBC data were analyzed using the Kruskal-Wallis test and continued with the Mann-Whitney Post Hoc test. Result: One-Way Anova test showed that MIC had a significant difference, and the Kruskal-Wallis test showed that MBC also had significant differences. MIC was obtained at the concentration of 20 mg/ml and MBC was obtained at the concentration of 30 mg / ml. Conclusion: There is antibacterial effectiveness in kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis.

scholarly journals PERBEDAAN EFEKTIVITAS EKSTRAK RIMPANG TEMULAWAK (Curcuma xanthorriza Roxb.) DENGAN EKSTRAK DAUN SALAM (Eugenia polyantha Wight) PADA PENURUNAN KADAR KOLESTEROL TOTAL TIKUS PUTIH JANTAN (Rattus norvegicus)

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Ayu Ulan Rizki ◽  
Cholid Cholid ◽  
Muttia Amalia
Keyword(s):  
Total Cholesterol ◽  
Leaf Extract ◽  
Disease Risk ◽  
Simple Random Sampling ◽  
Control Group ◽  
Control Group Design ◽  
Standard Drug ◽  
Cholesterol Levels ◽  
White Rats ◽  
Ginger Rhizome

Dyslipidemia is a coronary heart disease risk factor. Dyslipidemia is characterized by the elevated levels of LDL, triglycerides, total cholesterol, and the decrease in HDL. Ginger rhizome (Curcuma xanthorriza Roxb.) and bay leaf (Eugenia polyantha Wight.) are local plants that have the abilities to decrease total cholesterol. This research design was true experimental with pre- and post-control group design. Criteria of sample were white rats, wistar strain, male, age of 8-12 weeks, and body weight range of 150-200 grams. Sampling method was simple random sampling. The experimental rats were acclimatized for seven days. Hypercholesterol diet was fed two weeks, and then the rats were given intervention and get hypercholesterol diet for two weeks. During intervention, the experimental rats were divided into 6 groups of administrationi.e, rats administered with 1) carboxymethil celluse/CMC, 2) simvastatin 0,9 mg/kg BW/day, 3) ginger rhizome extract 200 mg /kg BW/day, 4) ginger rhizome extract 400 mg/kg BW/day, 5) bay leaf extract 200 mg/kg BW/day, and 6) bay leaf extract 400 mg/kg BW/day. Blood samples were taken from caudal vein and total cholesterol levels were measured by spectrophotometry. The analysis used paired T test, One Way ANOVA test. The results showed ginger rhizome extract did not effectively reduce total cholesterol. However, bay leaf extract effectively decreased total cholesterol levels with the highest total cholesterol reduction was found in rats administered with dose of 400 mg/kg BW/day i.e, 15.4 mg/dl. Bay leaf extract have the same effectivity with simvastatin as a standard drug in lowering cholesterol level.

scholarly journals Effect of Jati Belanda Leaves Extract on Myeloperoxidase Level in Wistar Rat's Lung Induced Contusion Pulmonum

2019 ◽  
Vol 5 (3) ◽  
pp. 116-120
Author(s):  
Nia Savitri Tamzil ◽  
Evi Lusiana ◽  
Desi Oktariana
Keyword(s):  
Leaf Extract ◽  
Positive Control ◽  
Lung Damage ◽  
Control Group ◽  
Alveolar Cells ◽  
Pulmonary Tissue ◽  
Control Group Design ◽  
White Rats ◽  
Guazuma Ulmifolia

Pulmonary contusions are injuries to the lung parenchyma that often result from blunt trauma to the chest wall. This injury will activate the inflammatory response which can produce the effects of oxidative stress so that eventually lung damage occurs. Several studies have identified the effects of Jati Belanda leaves extracts related to the inflammatory process and their effects as antioxidants. This research is an in vivo experimental study with a prepost-test with control group design approach that aims to determine the effectiveness of the extracts of Jati Belanda (Guazuma ulmifolia) in its protection against pulmonary alveolar cells by pretreatment pulmonary contusions. The subjects of this study were white rats (Rattus norvegicus) Wistar strain which were divided into 5 groups namely positive control, negative control, Dutch teak leaf extract 125 mg / kgBB, 250 mg / kgBB and 500 mg / kgBW. Rats were induced by dropping a weight of 400 grams as high as 50 cm. The results of the study of Jati Belanda Leaf Extract (EDJB) all doses and positive control can significantly reduce levels of myeloperoxidase expression (p &lt;0.05). Therefore, it can be concluded that the extracts of Jati Belanda leaves (Guazuma ulmifolia) are all effective doses in reducing the expression of myeloperoxidase in pulmonary tissue of Wistar rats induced by pulmonary contusions.

scholarly journals PERBEDAAN KEMAMPUAN BERPIKIR KREATIF BIOLOGI SISWA ANTARA YANG MENDAPAT PEMBELAJARAN MODEL PROBLEM BASED LEARNING (PBL) DENGAN MODEL INKUIRI DI SMP NEGERI 1 TANJUNG KEMUNING

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Detah Detah
Keyword(s):  
Creative Thinking ◽  
Problem Based Learning ◽  
Control Group ◽  
Control Group Design ◽  
Grade 5 ◽  
Quasi Experimental ◽  
Class Viii ◽  
The One ◽  
Post Hoc ◽  
Pbl Model

This research aims to look at differences in the ability of creative thinking among student who obtained Problem Based Learning (PBL) model, inquiry and conventional. The research was conducted on April – Mei, 2013 in SMP Negeri 1 Tanjung Kemuning, on the subjects of biology class VIII semester academic year 2012/2013. The method used in this study was quasi experimental. The design study is Pretest-Postest Control Group Design. Samples in this study were students in grade 5 class amount, taken 3 homogen classes using pretest skor. Research data collection using an test instrument, as for the data analysis techniques are us to test the hypothesis in this study is to use the One Way Anova and Post-Hoc Test using LSD. Abilities of student who obtain a Problem Based Learning (PBL) model moss is a 12,96 and inquiry model is a 12,12. There were no significant differences in the result of creative thinking abilities of student who obtain PBL model and inquiry model. There are significant differences in the result of creative thinking abilities of student receiving learning PBL model and inquiry model with conventional model. Keywords: PBL model, Inquiry model, Creative Thinking

scholarly journals Pengaruh Model Pembelajaran Search, Solve, Create, and Share dan Predict Observe Explain terhadap Hasil Belajar Biologi Siswa Kelas VIII SMPN 1 Gondangrejo Karanganyar Tahun Ajaran 2013/2014

2015 ◽  
Vol 26 (1) ◽  
pp. 11-20
Author(s):  
Djumadi Djumadi ◽  
Erfan Budi Santoso
Keyword(s):  
Learning Model ◽  
Cognitive Domain ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
The Third ◽  
Quasi Experimental ◽  
The Difference ◽  
Post Hoc ◽  
And Control

This study has objectives (1) to know the effect of SSCS learning model and POE toward the achievement of students learning biology, (2) the difference between the achievement of students learning biology using SSCS learning model and POE. This is quasi experimental research with randomized subjects posttest only control group design. The population are three classes randomly selected from SMP Negeri 1 Gondangrejo Karanganyar 2013/2014. The first class, VIIID, is thaught by POE learning model. The second class is SSCS learning model, and the third, as control class, is thaught by variative lecturing. Data are collected by test, quessionaire, observation, and documentation.Then data are analysed by validity, reliability, difficulty test index. The results are (1) there is an effect of SSCS learning model and POE toward the achievement of students learning biology at three aspects: cognitive, affective and psychomotoric. It can be seen on the average of students learning achievement at experiment class and control class with the significance value < 0,05. The proceed result of anova Post Hoc test Scheffe indicates that there is a difference the achievement of students learning biology using SSCS learning model and POE at affective and psichomototic domain, but it does not happen in cognitive domain. The conclusion is (1) there is an effect of the achievement of students learning biology using SSCS learning model and POE, (2) SSCS learning model is more effective than POE.

scholarly journals PENGARUH DOSIS EKSTRAK JAMBU BIJI (PSIDIUM GUAJAVA. L) DAN TABLET Fe TERHADAP PERUBAHAN KADAR HEMOGLOBIN PADA MENCIT (MUS MUSCULUS)

2018 ◽  
Vol 10 (3) ◽  
pp. 102-107
Author(s):  
Yusnaini Yusnaini
Keyword(s):  
Mus Musculus ◽  
Experimental Studies ◽  
Psidium Guajava ◽  
Control Group ◽  
Control Group Design ◽  
Laboratory Design ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Post Hoc

According to the WHO 40% of maternal deaths in developing countries related to anemia in pregnancy and is most commonly caused by iron deficiency and acute bleeding, even less so the two interact. This study aims to determine the effect of doses of extracts of Guava (Psidium Guajava. L) and Tablet Fe to changes in hemoglobin levels in mice (Mus musculus). Type True Experimental studies in laboratory design nonrandomized design Pretest-Posttest Control Group Design. Samples were mice (Mus musculus) as many as 20 birds were divided into four groups with each group number as many as five tails. Group A was given a tablet Fe as much as 0.126 mg / kg / day, group B was given a tablet Fe as much as 0.126 mg / KgBW / day coupled with extracts of Guava as much as 10 mg / kg / day, group C was given tablets Fe as much as 0.126 mg / kg / day coupled with guava extract as much as 20 mg / kg / day, group D was given tablets as much as 0.126 mg Fe / KgBW / day plus guava extract as much as 30 mg / kg / day. The data collection was conducted from June 5 s / d July 20, 2015. Data were analyzed using Paired T-Test continued with Test and One Way ANOVA Post Hoc Test namely LSD test. Results showed that there was no significant difference in hemoglobin levels between the groups in which the value of P = 0634> 0.05.. Based on the results of this study concluded that there was no effect of dose of extract of guava (Psidium Guajava. L) and Tablet Fe to changes in hemoglobin levels in mice (Mus musculus), but the effect on the number of erythrocytes of mice (Mus musculus)

scholarly journals Interdisciplinary Approach-Based Energy Education

2019 ◽  
Vol 9 (1) ◽  
pp. 57
Author(s):  
Gokhan Guven ◽  
Yusuf Sulun
Keyword(s):  
Academic Achievement ◽  
Science Teachers ◽  
Conceptual Understanding ◽  
Interdisciplinary Approach ◽  
Service Science ◽  
Control Group ◽  
Control Group Design ◽  
Energy Education ◽  
Quasi Experimental ◽  
Post Hoc

The purpose of the current study is to investigate the effect of interdisciplinary approach-based energy instruction on pre-service science teachers’ academic achievement and conceptual understanding regarding the concept of energy. To this end, the current study was designed as a quasi-experimental study in line with the pretest-posttest control group design. The activities related to the concept of energy were conducted by using the interdisciplinary approach and the same activities were conducted by using the methods and techniques based on the existing approaches. A total of 66 pre-service science teachers participated in the study lasting for 10 weeks. In the analysis of the data, ANOVA/Post Hoc Test was used. As a result of the study, it was found that the use of the interdisciplinary instructional approach in teaching the concept of energy increased the pre-service teachers’ academic achievement and conceptual understanding more than the traditional approaches. Thus, it can be suggested that during the instruction of the energy concept, the features of this concept should be taught in a certain developmental order and the interdisciplinary approach should be used in the activities conducted to teach this concept.

scholarly journals Pengaruh Pemberian Kopi Instan Oral Terhadap Kadar Asam Urat pada Tikus Wistar

2014 ◽  
Vol 3 (3) ◽  
Author(s):  
Fauzan Arisyi Koto ◽  
Husnil Kadri ◽  
Zelly Dia Rofinda
Keyword(s):  
Uric Acid ◽  
Coffee Consumption ◽  
Control Treatment ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
White Rats ◽  
Wide Range ◽  
Post Hoc ◽  
One Way Anova

AbstrakKopi merupakan salah satu minuman yang paling banyak dikonsumsi di dunia. Banyak studi yang meneliti efek konsumsi kopi terhadap berbagai kondisi medis tertentu. Salah satu efek dari kopi yang masih menjadi kontroversi adalah efek terhadap penurunan kadar asam urat. Kandungan polifenol dalam kopi diduga dapat menghambat kerja xantin oksidase sehingga menurunkan kadar asam urat. Tujuan penelitian ini adalah untuk menentukan pengaruh pemberian kopi oral terhadap kadar asam urat serum pada tikus wistar. Ini adalah penelitian eksperimental dengan rancangan post test only control group design. Sampel penelitian adalah 24 ekor tikus putih jantan (Rattus novergicus) yang dibagi menjadi 4 kelompok yaitu kelompok kontrol, perlakuan 1, perlakuan 2 dan perlakuan 3. Kontrol hanya diberi diet standar tanpa kopi, perlakuan 1diberikan diet kopi dosis rendah setara 3 cangkir kopi (0,39 mg/3 ml), perlakuan 2 diberikan diet kopi dosis sedang setara 6 cangkir kopi (0,78 mg/ 3ml), perlakuan 3 diberikan diet kopi dosis tinggi setara 10 cangkir kopi (1,3 mg/ 3ml) selama 4 minggu (28 hari). Pengukuran kadar asam urat serum menggunakan spektofotometer. Hasil penelitian didapatkan rerata kadar asam urat serum kontrol (2,26+0,16 mg/dl), perlakuan 1 (2,24+0,89 mg/dl), perlakuan 2 (1,00+0,33 mg/dl), perlakuan 3 (1,96+0,43 mg/dl). Uji analisis one way Anova dan Post hoc menunjukkan bahwa perbedaan yang bermakna hanya terdapat pada perbandingan kelompok kontrol dengan kelompok perlakuan 2 dan antara kelompok perlakuan 1 dengan kelompok perlakuan 2 (p<0,05). Kesimpulan dari penelitian ini adalah terdapat penurunan kadar asam urat serum setelah pemberian kopi dan terdapat perbedaan bermakna kadar asam urat antara kelompok kontrol dan perlakuan 1 dengan kelompok perlakuan 2.Kata kunci: kopi, polifenol, asam urat serumAbstractCoffee is one of the most frequently consumed beverages in the world. Many studies have examined the effect of coffee consumption on a wide range of specific medical condition. One of the effects of coffee that still a controversy is the effect of the reduction in uric acid levels because the polyphenol in coffee could be expected to inhibit xanthine oxidase to lower uric acid level. The objective of this study was to determine the effect of coffee on uric acid levels Wistar strain of rats (Rattus novergicus). This was a experimental study with posttest only control group design. Samples were 24 male white rats strain Wistar divided into 4 groups: control, treatment 1, treatment 2, and treatment 3. Control was not treat with coffee, treatment 1 were given low dose of coffee equivalent to 3 cups (0,39 mg/3 ml), treatment 2 were given moderate dose of coffee equivalent to 6 cups (0,78 mg/ 3ml), treatment 3 were given high dose of coffee equivalent to 10 cups (1,3 mg/ 3ml) for 4 weeks (28 days). Serum uric acid levels measured with sphectrophotometer The result showed the mean of uric acid levels control (2,26+0,16 mg/dl), treatment 1 (2,24+0,89 mg/dl), treatment 2 (1,00+0,33 mg/dl), treatment 3 (1,96+0,43 mg/dl). One way Anova dan Post hoc tests analysis showed significant differences of uric acid levels only between control and treatment 2 group and between treatment 1 group and treatment 2 group (p<0,05). The conclusion of this study is reduction of uric acid levels after coffee consumption and the significant differences in uric acid levels between control/treatment 1 group with treatment 2 group.Keywords: coffee, polyphenol, uric acid levels

Sign in / Sign up

Export Citation Format

Share Document