scholarly journals VARIABILITY OF COMPONENT COMPOSITION OF MELISSA OFFICINALIS L. ESSENTIAL OIL DEPENDING ON THE CLIMATIC CONDITIONS OF THE REGION OF CULTIVATION

2020 ◽  
pp. 257-263
Author(s):  
Natal'ya Vladimirovna Nevkrytaya ◽  
Vladimir Stepanovich Pashtetskiy ◽  
Ilya Aleksandrovich Novikov ◽  
Irina Nikolaevna Korotkikh ◽  
Ruslan Ramazanovich Tkhaganov

The aim of the research was to detect the dependence of content and component composition of Melissa officinalis L. essential oil from climatic peculiarities of the region of cultivation and to specify the optimum conditions for growing high-quality raw materials. We presented the results of the comparative assessment of essential oil component composition (three main elements) obtained from air-dried raw materials of two cultivars Krymchanka and Lada. These Melissa officinalis L. cultivars were grown in three regions that sharply differed in weather conditions. To make the comparison accurate, we determined the component composition of essential oil in the Laboratory of Biochemistry at FSBSI “Research Institute of Agriculture of Crimea” using gas chromatograph Kristall 5000.2. The most favorable climatic conditions for essential oil accumulation were in the Foothill zone of the Crimea (moderate rainfalls combined with high air temperatures). Cultivar Krymchanka exceeded cultivar Lada by the amount of essential oil in raw materials. Excess sometimes reached 170%. Dependence of the main components such as citral, β-caryophyllene, caryophyllene oxide, germacrene D from the ratio of temperature and precipitation was revealed. After obtaining research results, we could suggest that by controlling the conditions of cultivation of M. officinalis, it is possible to adjust the ratio of the components of essential oil.

2020 ◽  
Vol 202 (11) ◽  
pp. 76-84
Author(s):  
Ol'ga Pehova ◽  
Lidiya Timasheva ◽  
Irina Danilova ◽  
Irina Belova

Abstract. The purpose of the research was to study the quality of Elsholtzia stauntonii Benth. raw materials grown in the foothill zone of the Crimea. In the course of the research, we studied such indicators as the content of essential oil and other types of biologically active substances (BAS), which allow using elsholtzia as an essential oil, food, or medicinal raw materials. Research methods. Determination of the quality of E. stauntonii raw materials was carried out according to generally accepted methods. Results. Peculiarities of the accumulation of essential oil in various organs of E. stauntonii were determined. The variability of the mass fraction of essential oil, its component composition and BAS depending on the plant’s growth stage is shown. We found that the main oil-synthesizing organs of E. stauntonii are leaves and inflorescences. The largest amount of essential oil (1.82 %) was obtained from inflorescences. In the fractional composition of raw materials, they amounted to 45.6 %. The maximum amount of essential oil with the dominant components (rosefuran and rosefuran epoxide) accumulated in plants during the phase of mass flowering (1.48 % in terms of absolutely dry weight). The highest content of BAS, namely phenolic compounds, accumulated in the stage of active growth of vegetative and the formation of generative organs of plants (regrowth and bud formation); extractives – in the stage of regrowth. During two years of storage of air-dry raw materials, there is a loss of essential oil as a result of its evaporation (54.0 %) and a change in its component composition (an increase in monoterpene derivatives of furan by 26.6 % and a decrease in sesquiterpene hydrocarbons by 23.5 %). To store air-dry raw materials of E. stauntonii for more than two years is not, however, reasonable because of significant losses of essential oil. Scientific novelty. The quality of raw materials of E. stauntonii variety Rozovoe oblako, which was grown in the foothill zone of the Crimea, was studied for the first time. Raw materials and essential oil of E. stauntonii have a broad spectrum of biological activity and can be used in medicine, as well as in essential oil and food industries.


Author(s):  
O. M. Shevchuk ◽  
S. P. Lukashuk ◽  
S. A. Feskov ◽  
S. L. Adzhiakhmetova ◽  
N. M. Chervonnaya ◽  
...  

The article provides data on a comparative study of the content and component composition of essential oil, the total content of antioxidants and flavonoids of two cultivars and two promising cultivar samples of Italian strawflower ( Helichrysum italicum (Roth) G. Don) grown on the Southern Coast of the Crimea in the collection of aromatic and medicinal plants of the Nikitsky Botanical Gardens. It has been found that the content of essential oil in the green weight of top of H. italicum varies from 0.1% of the wet weight (‘Crystal’ cultivar) to 0.2 (cultivar No. 5). The main components of ‘VIM’ cultivar essential oil are α-pinene and D-limonene, of ‘Crystal’ cultivar is neril acetate, of the sample No. 5 is α-pinene and rosiforiol, of the sample No. 158 is neryl acetate and γ-turmeric. A significant relative content of β-diketones (38%) in the essential oil of ‘Crystal’ cultivar was noted. The greatest amount of antioxidants (2.59 mg / g (in conversion to quercetin) and flavonoids (0.366%) in the inflorescences of Italian strawflower ‘VIM’ cultivar is contained in the budding phase. The results of chromatographic analysis prove the presence of 4 phenolic substances in the inflorescences of Italian strawflower. UV-spectra of alcohol solutions of the studied raw materials had a maximum absorption at a wavelength of 418 ± 2nm. Quantitative determination of flavonoids by differential spectroscopy calculated as isosalipurposide showed that the content of flavonoids in inflorescences varies from 0.262% to 0.366% depending on a cultivar and a phase of vegetation.


2020 ◽  
pp. 39-53
Author(s):  
Tatyana Shcheglova

The article presents results of the study of a comparative analysis of essential oil obtained from fresh and dried leaves of medicinal sage (Salvia officinalis L.), growing in the Botanical Garden of the First Moscow State Medical University named after Sechenov. The studies were carried out using gas chromatography– mass spectrometry. Qualitative and quantitative difference in the component composition of the essential oil obtained from fresh and dried raw materials was determined as a result of the study.


2021 ◽  
Vol 36 ◽  
pp. 01003
Author(s):  
Elena Vologzhanina ◽  
Galina Batalova

The results of the study of 12 varieties and promising lines of glumaceous oat in the competitive variety testing of the FASC of the North-East (Kirov region) in the period from 2018 to 2020 are presented. The purpose of the research is to assess the productivity, ecological plasticity and stability of the genotypes of glumaceous oat for feed and universal use in the conditions of the Volga-Vyatka region. The dependences of grain yield and dry matter harvesting on the state of agro-climatic resources (HTC), temperature and precipitation are established. The contrasting weather conditions during the years of research allowed to conduct the most complete assessment of the studied genotypes. The most favorable conditions for the formation of a high yield of green mass of oat were formed in 2020 (Ij=3.76), grain - in 2019 (Ij=1.35). The average degree of positive dependence of green mass yield on the height of oat plants was revealed (r=0.51). The variety of the high-intensity type of the universal direction Medved, promising lines of the mowing direction (178h13 and 245h14) are distinguished.


2018 ◽  
Vol 9 (3) ◽  
pp. 340-346 ◽  
Author(s):  
T. E. Talankova-Sereda ◽  
J. V. Kolomiets ◽  
A. F. Likhanov ◽  
A. V. Sereda ◽  
N. I. Kucenko ◽  
...  

Quantitative and qualitative composition of essential oils of peppermint breeds Lebedinaya Pesnya, Lubenchanka, Lidiya, Ukrainskaya Perechnaya, Mama, Chornolista was investigated before and after clonal microreproduction by the method of isolated tissues and bodies culture in vitro. Methods of essential oil steam distillation, capillary gas chromatography and statistical analysis were used in the research. It is established that increase in essential oil quantity was observed for peppermint breeds on which reproduction and improvement іn vіtro technology was applied. As a result of clonal microreproduction of peppermint plants in culture іn vіtro on nutrient medium Murasige and Skug, in which the growth regulators 0.75 mg/l of 6-benzylaminopurine, 0.1 mg/l of adenine, 0.05 mg/l of indolil-3-acetic acid and 0.5 mg/l of gibberellins acid were added and virocide Ribavirin in concentration 10 mg/l, improvement was obtained in comparison with vegetatively reproduced plants; increase in essential oil quantity per hectare was established for the following breeds; Chornolista by 54.2%, Lebedinaya pesnya by 38.2%, Ukrainskaya Perechnaya by 36.7%, Mama by 28.5%, Lubenchanka by 17.1% and Lidiya by 11.6%. For oil content the highest indices were noted for Lubenchanka, Mama and Lebedinaya Pesnya peppermint breeds with product yield 4.02%, 3.98% and 3.84% respectively. It was established that the essential oil component composition in non-clonal peppermint plants raw materials and plants-regenerants after culture in vitro is variable depending on breed. Limonene, cineole, menthone, menthofuran, iso-menthone, menthyl acetate, β-caryophyllene, iso-menthol, menthol, pulegone, germacren, piperitone, carvone were identified in peppermint essential oil. High content of menthol, low content of carvone, piperitone, pulegone (except for Chornolista, Ukrainskaya Perechnaya breeds) and menthofuran (except for Chornolista, Ukrainskaya Perechnaya and Lubenchanka breeds) are characteristic for Ukrainian selection peppermint investigated breeds. A clear tendency to menthol and menthone content ratio increase is observed in plants which were improved in conditions іn vіtro. Pulegone was not detected in essential oil samples of Lebedinaya Pesnya, Lidiya and Mama breeds. Biochemical markers of Lebedinaya Pesnya, Lubenchanka, Mama breeds, which differentiate them within the group of investigated breeds, are higher limonene, piperitone and menthol pool; for Ukrainskaya Perechnaya and Chornolista breeds – pulegone, cineole and menthone; for Lidiya breed – iso-menthone.


Author(s):  
N.V. Nevkrytaya ◽  
◽  
I.A. Novikov ◽  

In 2017–2019, a competitive variety trial of promising cultivar MD 1-17 of Melissa officinalis L. subsp. Altissima (Smith.) Arcang was conducted. We compared it with M. officinalis L. subsp. Officinalis varieties ‘Krymchanka’ and ‘Lada’. Сultivar MD 1-17 significantly exceeds other varieties in terms of yield of fresh plant material (on average, by 77.4 and 62.2 %, respectively). It also surpasses variety ‘Krymchanka’ (the best in collecting essential oil from air-dried raw materials) by 57.1 %. Basic components presented in the essential oil of M. officinalis L. subsp. Altissima (Smith.) Arcang. cv. MD 1-17. Promising cultivar are caryophyllene (25.3–35.9 %) and germacrene D (17.7–31.2 %). Citral is almost completely absent or present in an insignificant amount (0.1–7.3 %); its proportion in the essential oil of varieties ‘Krymchanka’ and ‘Lada’ can reach 36.6 %.


2020 ◽  
Vol 9 (4) ◽  
pp. 142-148
Author(s):  
Nadezhda Vasilyevna Portnyagina ◽  
Vasiliy Vitalyevich Punegov ◽  
Elmira Elizbarovna Echishvili ◽  
Marina Gennadyevna Fomina ◽  
Ivan Vladimirovich Gruzdev

The paper presents the results of the research on the growth, development, and biochemical evaluation of introduced Pyrethrum majus plants in the middle taiga subzone of the Komi Republic. It is established that in the conditions of culture the species is characterized by a high stability and winter hardiness. The phenology and dynamics of plant growth were studied. Morphological parameters of vegetative and generative shoots were determined. The yield of essential oil from the aboveground phytomass of P. majus and its component composition were studied. It was found that the content of essential oil of flowering plants varied over the years of research from 0,25 to 1,41% and depended on the weather conditions of the growing season. More than 190 components were found in the essential oil of P. majus plants using the method of GLC analysis, 48 of them were identified. The main terpenoids in the essential oil were S-(+) carvone (5465%), -tuyon (610%), TRANS-and CIS-p-Menta-2,8-dienols (2,32,8%), 1,8-Cineol (1,42,9%), CIS Menta-1(7)8-Dien-2ol (1,52,1%), germacrene d (1,21,8%) -cadinol (0,51,5%), -muurolol (0,22,6%). It was found that the component composition of the essential oil from perennial plants P. majus corresponds to the carvone-Tuyon chemotype and did not change significantly due to the meteorological conditions of the environment. Taking into account a high adaptation of plants of this species to the conditions of the middle taiga subzone of the Komi Republic, high indicators of aboveground phytomass with a sufficient content of essential oil, P. majus can be successfully cultivated in the North as a promising spicy-aromatic medicinal and ornamental plant.


Author(s):  
Т. Балдан

Unique biological quality of Mongolian livestock that helps the animal withstand the cold and feed shortage seasons in despite of feeding pasture plants only for all year round is an evidence of its pasture usability. Better growth of hair and wool of hardy Mongolian livestock, which are able to grow under severe natural and climatic conditions, during summer and autumn, and fibers appeared around the hair base during cold season make the animals resistant to cold. Mongolia has a long tradition of raising livestock in the vast territory. This is based on a system of pastoral herding consisting of five kinds of livestock. Mongolians have specific, historical traditions of nomadic ways for pastoral animal breeding. Since ancient times they are bred the five kinds of livestock with unique capabilities, adapted to four seasons grazing under harsh natural and climatic conditions of Central Asian mountainous and used their benefits. Although Mongolian livestock breeds are well adapted to harsh weather conditions, their productivity is not especially high. Animal husbandry is the fundamental source of food consumption and raw materials for the national economy, contributing 20% of the total GDP. Livestock workers make up 34.5% of the total labor force. The livestock sector is responsible for 90% of total agricultural production and comprises 12.5% of all exports. Currently, Mongolia has: 2 breeds and 2 strains of horses, 4 breeds of camel, 3 breeds and 1 breeding group of cattle, 13 breeds, 3 breed groups and 3 breeding races and 3 strains of sheep and 7 breeds, 4 strains of goats forming genetic resources of Mongolian livestock. The main products of the livestock sector are meat, milk, wool, cashmere and hides. Due to the harsh climatic conditions of Mongolian livestock, their wools grow well in the summer and autumn, and during the cold season, cashmere is covered with cashmere and they are resistant to cold. Mongolians have specific historical traditions of managing nomadic husbandry of five types of livestock populations, which are adapted to all year round grazing under severe natural and climatic conditions of Central Asian plateau, growing and breeding of wealth producing livestock populations and utilizing their animal products since ancient times.


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