scholarly journals Optimasi Produksi Inulinase oleh Khamir Pichia manshurica DUCC Y-015 pada Tepung Umbi Dahlia (Dahlia variabilis Willd.) dengan Variasi Konsentrasi K2HPO4 dan Waktu Inkubasi

2016 ◽  
Vol 18 (2) ◽  
pp. 48
Author(s):  
Inggrit Amedia ◽  
Wijanarka Wijanarka ◽  
Susiana Purwantisari

Sugar national supply more and more decreases and can not meet the market needs. The research has been done to find alternative natural sweeteners including inulin from dahlia tubers (D. variabilis Willd). Dahlia tuber can produce 95% of yield of fructose syrup in an enzimatic reaction by inulinase (E.C.3.2.1.7). Inulinase is inductive enzyme that can be produce by P. manshurica. The production of fructose needs to be optimized to get optimum product. The optimization can be done by modifying the nutrient content in the medium such as K2HPO4 and variation of incubation time. The purpose of this study is to determine the concentration of K2HPO4 and optimum incubation time for P. manshurica. This research was conducted in Microbiology Laboratory, Biology Department, Faculty of Science and Mathematics Undip. The examined variable is the growth of yeast cell, inulinase activity, invertase, and the I/S ratio. This research was conducted experimentally using Randomized Complete Block Design (RCBD) factorial pattern with 2 factors, the first factor was the concentration of K2HPO4 (P), with concentration  level (g/L) of 0.5 (P1), 1.0 (P2), and 1.5 (P3). The second factor was incubation time (W) with 12 hours (W12), 18 hours (W18), and 24 hours (W24). Every treatment was repeated three times. The collected data were analyzed using ANOVA. If there was a treatment effect, it will be continued with Duncan test on 5% significance level. The result of analysis show that the highest cell growth and the maximum production of inulinase enzyme was in P3W24 occurs in P3W24 (K2HPO4 1.5 g/L and 24 hours incubation time) treatment at 0.428 IU, but efficient in P1W12 treatment as much as 0.365 IU. Keywords: Dahlia variabilis Willd., Inulinase, K2HPO4, Pichia manshurica DUCC Y-015, incubation time

Author(s):  
M Alvin Rifqi Fadilla, Erwanto, Muhtarudin, dan Agung Kusuma Wijaya

The purpose of this research was to determine the effect of addition of Multinutrient Sauce (MNS) in the ration on digestibility of crude protein and crude fiber in sheep.  This research was conducted in May—July 2019 at the People's Farm of Kebagusan Village, Gedong Tataan District, Pesawaran Regency.  The digestibility analysis of crude protein and crude fiber was carried out at the Laboratory of Animal Nutrition and Feed, Animal Husbandry Department, Faculty of Agriculture, University of Lampung.  This study used a randomized block design (RBD) based on sheep body weight with six groups and three treatments, namely R0 (ration without MNS); R1 (R0 + MNS 5%); and R2 (R0 + MNS 10%).  The data obtained were analyzed using ANOVA at a 5% significance level or 1% significance, and continued with further test using Least Significant Difference Test (LSD) if there is significant results.  The results showed that the addition of 10% MNS in the ration had a significant effect (P<0.05) on increasing the digestibility of crude protein and crude fiber in sheep.   Keywords: multi nutrient sauce (MNS), digestibility of crude protein, digestibility of crude fiber, sheep.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 516b-516
Author(s):  
James N. McCrimmon

Zoysiagrass has great potential for use in the Gulf Coast states as a turfgrass. There has been minimal research on the nitrogen (N) and potassium (K) fertility response of zoysiagrass and the effect on turf color, quality, and nutrient content. The objective of this study was to evaluate the effects of N and K fertility on zoysiagrass. A study was conducted on three zoysiagrasses: Zoysia japonica × Z. tenuifolia Willd. ex Trin. (`Emerald'); Z. japonica Steud. (`Meyer'); and Z. matrella. The N and K treatment combinations consisted of high (H) and low (L) rates of N and K at the following levels: N levels of 454 and 227 g N/92.9 m2 per month and K levels of 454 and 227 g N/92.9 m2 per month. The treatment combinations were (N and K): HH, HL, LH, and LL and were applied in two split applications monthly from July through November. The study was a randomized complete-block design with three replications. All plots received two applications of a micronutrient fertilizer (late June and August), were irrigated as needed, and maintained at a height of 3.8 cm. Color, density, texture, uniformity, and quality were determined visually for each month. Plant tissue samples were collected (September) and analyzed for macronutrient and micronutrient contents. There were significant differences for color, density, and quality in the following months: September (color and density); October (quality); and November (color and quality). There were differences in leaf texture for all months. There were significant differences for N, magnesium (Mg), and K contents but there were no differences for any micronutrient. This study indicated that all three zoysiagrasses provided acceptable color and quality during the summer and fall, and that N and K rates affected N, K, and Mg contents in the plant.


Author(s):  
Bistok Hasiholan Simanjuntak ◽  
Devi Kusuma Wardani

Robusta coffee plant propagation is recommended to be performed by vegetative methods, one of which is cuttings. Among plant organs that can be used for cuttings is the stem. The research objective was to determine which stem segment from cuttings was able to provide the best root and leaf growth. The layout study used a completely randomized block design (RBD) with an experimental treatment consisting of five stem segments, namely (1) the first stem segment from the upper shoot (A); (2) the second stem segment from the upper shoot (B); (3) the third stem segment from the upper shoot (C); (4) the fourth stem segment from the upper shoot (D); and (5) the fifth stem segment from the upper shoot (E); each treatment had five replications. Data analysis was performed statistically with analysis of variance (ANOVA) and Tukey's HSD (honestly significant difference) test with a significance level of P < 0.05. Robusta coffee cuttings stems from the second stem segment from the upper shoot were able to provide the optimal root growth (root length) and shoot growth (leaf sprout height and leaf sprout growth rate). Leaf sprout number derived from the cuttings was not affected by the stem segment.


AgriPeat ◽  
2019 ◽  
Vol 20 (01) ◽  
pp. 19-26
Author(s):  
Admin Journal

ABSTRACTThe role of Trichocompost and KCl fertilizer to control Fusarium wilt disease on onion in sandy soil. Fusarium wilt on onion is an interesting disease it is can loss the onion yield. The purpose of research to study trichocompost and KCl fertilizer role to control Fusarium wilt disease on ann onion. The research design used a Factorial Randomized Block Design with 2 factors. The first factor is 4 levels trichocompost, it is: without trichocompost (T0), trichocompost 10 t.ha-1 dosage (T1), trichocompost 20 t.ha-1 dosage (T2), trichocompost 30 t.ha-1 dosage (T3). The second factor is 3 levels KCl fertilizer, it is: without KCl (K0), KCl 100 kg.ha-1 dosage (K1), KCl 200 kg.ha-1 dosage (K2). Result of this research showed the application of trichocompost 10 t.ha-1 dosage and KCl 100 kg.ha-1 dosage can inhibit Fusarium wilt incubation time, can inhibit the patogen development with effective value 89,23%, the single factor it is aplication trichocompost 10 t.ha-1 dosage and trichocompost 30 t.ha-1 dosage not significant to dried onion bulb weight per clump of onion plant.Key words: Trichocompost, KCl fertilizer, Fusarium wilt disease, onion, sandy soil.ABSTRAKPenyakit layu Fusarium merupakan salah satu penyakit penting dapat menurunkan produksi bawang merah hingga 50%. Tujuan penelitian untuk mengetahui peranan trichokompos dan pupuk KCl dalam mengendalikan penyakit layu fusarium pada tanaman bawang merah. Penelitian menggunakan Rancangan Acak Kelompok faktorial dua faktor perlakuan. Faktor pertama 4 taraf dosis trichokompos yaitu: tanpa trichokompos (T0), trichokompos dosis 10 t.ha-1 (T1), trichokompos dosis 20 t.ha-1 (T2), trichokompos dosis 30 t.ha-1 (T3). Faktor kedua 3 taraf dosis pupuk KCl yaitu: tanpa pupuk KCl (K0), pupuk KCl dosis 100 KCl kg.ha-1 (K1), pupuk KCl dosis 200 KCl kg.ha-1 (K2). Hasil penelitian menunjukkan pemberian trichokompos 10 t.ha-1 dan pupuk KCl 100 kg.ha-1 dapat memperpanjang masa inkubasi penyakit, menekan serangan penyakit layu Fusarium dengan nilai efektivitas sangat baik (89,23%), perlakuan tunggal trichokompos dosis 10 t.ha-1 tidak berbeda nyata dengan dosis 30 t.ha-1 terhadap bobot umbi kering per rumpun tanaman bawang merah.Kata kunci: penyakit layu Fusarium, pupuk KCl, tanah berpasir, tanaman bawang merah, trichokompos.


2017 ◽  
Vol 39 (2) ◽  
pp. 137
Author(s):  
Marielly Maria Almeida Moura ◽  
Daniel Ananias de Assis Pires ◽  
Renê Ferreira Costa ◽  
Daniella Cangussú Tolentino ◽  
João Paulo Sampaio Rigueira ◽  
...  

 This study aimed to evaluate the nutritional characteristics and quality of silages of five sorghum genotypes, namely: Volumax, AG2005E, Qualimax, BRS610 and AG2501. The study was conducted at the Experimental Farm Unimontes, in the municipality of Janaúba, state of Minas Gerais. The treatments were distributed in a randomized block design with four replicates. Data were tested by analysis of variance andmeans were compared by Scott-Knott test at a significance level of 5%. The pH values ranged from 3.93 and 4.10. Genotypes differed in all studied nutritional characteristics (p < 0.05). For the acid detergent fiber, differences were detected between the genotypes, AG2501 (35.66%), Volumax (34.89%), AG2005E (34.53%), Qualimax (32.39%) and BRS610 (33.60%). The high participation of lignin was verifiedin all genotypes except for Volumax (4.14%). All silages were within the recommended range 50-65% for in vitro dry matter digestibility and classified as having good quality, however only the AG2005E genotype met nitrogen requirements for microbial fermentation, with 7.06% crude protein. 


CERNE ◽  
2015 ◽  
Vol 21 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Manoel Cléber de Sampaio Alves ◽  
Luiz Fernando Frezzatti Santiago ◽  
Marcos Tadeu Tiburcio Gonçalves ◽  
Ivaldo De Domênico Valarelli ◽  
Francisco Mateus Faria de Almeida Varasquim

The present paper aims to evaluate the influence of the factors (belt speed, pressure and grit size) on the output parameters (temperature and surface roughness) for Pinus elliottii wood sanding, processed parallel to the fibers. Three levels of belt speed, three levels of pressure and four levels of grit size were employed, with six replicates for each process, totaling 216 observations. The experiment conducted under a randomized complete block design (RCBD). The results were analyzed employing the analysis of variance (ANOVA) with 5% of significance level. Only grit sizes were significant to different temperature levels. The same outcome was observed for roughness, where only grits sizes were significant. No interaction between the pressure and belt speed factors were observed for all the results analyzed.


FLORESTA ◽  
1999 ◽  
Vol 29 (12) ◽  
Author(s):  
SIMONE APARECIDA DE OLIVEIRA ◽  
MARIO LUIZ TEIXEIRA DE MORAES ◽  
SALATIÉR BUZETTI

Sementes de aroeira foram obtidas a partir de 30 árvores de polinização livre, localizadas na Estação Ecológica do Instituto Florestal em Paulo de Faria - SP, em setembro de 1996. Foram instalados dois testes de progênies de aroeira (consorciado com mutambo e angico do campo – Exp 1 e homogêneo – Exp 2), em março de 1997, na Fazenda de Ensino e Pesquisa da FEIS/UNESP, em Selvíria - MS. O delineamento experimental utilizado foram os blocos casualizados, tanto no experimento consorciado como no homogêneo. Os caracteres nutricionais avaliados foram o conteúdo de: N, P, K, Ca, Mg e S. As estimativas de parâmetros genéticos foram obtidas em nível de média de parcelas. A herdabilidade, aos dois anos, variou de 0,05 (para o Mg) a 0,63 (para o N). Foi encontrada variabilidade genética para o teor de nutrientes e a característica mais indicada para um processo de seleção seria o conteúdo de N. Nutrient aspects of genetic variation on progeny of Myracrodruon urundeuva Fr. All. under different condition of crop Abstract Aroeira seeds were obtained from 30 trees of open pollination, located in the Estação Ecológica do Instituto Florestal in Paulo de Faria – SP, in September, 1996. Two tests of Aroeira progeny were conducted (mutambo and angico together – Exp 1 and homogeneous – Exp 2), in March, 1997, at experimental station of FEIS/UNESP, in Selvíria – MS. A randomized completely block design, to both experiments was used. The nutrient content evaluated was: N, P, K, Ca, Mg, and S. The genetic estimates achieved to mean plot level and herdability on 2 year old trees was 0,05 to Mg and 0,63 to N. There was genetic variability to nutrient content and N was the better characteristic to selection process.


Aksioma ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 13-18
Author(s):  
Fitrianti Fitrianti ◽  
Bakri Mallo ◽  
Linawati Linawati

Abstrak: Tujuan dari penelitian ini adalah untuk mengetahui hubungan antara kecerdasan emosional dengan prestasi belajar matematika siswa kelas VIII SMP Negeri 8 Palu. Populasi dari penelitian ini adalah seluruh siswa kelas VIII SMP Negeri 8 Palu yang terdiri dari 5 kelas. Sampel penelitian diperoleh dengan menggunakan teknik random sampling, jumlah siswa yang menjadi sampel sebanyak 44 orang. Pengumpulan data menggunakan dua instrumen yaitu, kuisioner kecerdasan emosional dan dokumentasi prestasi belajar dari nilai rapor. Hasil analisis data dengan menggunakan uji korelasi product moment diperoleh nilai korelasi r = 0,417. Nilai rtabel = 0,297 untuk N = 44 dengan taraf signifikansi 5%. Nilai rhitung (0,417) > rtabel (0,297) maka diputuskan menerima hipotesis alternatif (H1) dan menolak hipotesis awal (H0). Artinya terdapat hubungan yang positif dan signifikan antara kecerdasan emosional dengan prestasi belajar matematika siswa kelas VIII SMP Negeri 8 Palu. Kata kunci: Kecerdasan Emosional dan Prestasi Belajar Matematika The purpose of this study was to determine the relationship between emotional intelligence with mathematics learning achievement of VIII grade students at SMP Negeri 8 Palu. The population of this study is all students of VIII grade at SMP Negeri 8 Palu consisting of 5 classes. The sample was obtained by using random sampling technique. The total number of the sample was 44 students. The data collection used two instruments, namely, emotional intelligence questionnaire and documentation of learning achievement from the report card. The results of data analysis using product moment correlation test obtained correlation value r = 0.417. Value of rtable = 0,297 for N = 44 with significance level 5%. The value of rcount (0.417)> rtable (0.297) then decided to accept the alternative hypothesis (H1) and reject the initial hypothesis (H0). This means there is a positive and significant relationship between emotional intelligence and mathematics learning achievement of VIII grade students at SMP Negeri 8 Palu. Keywords: Emotional Intelligence and Mathematics Learning Achievement


2019 ◽  
Vol 6 (02) ◽  
pp. 81-88
Author(s):  
Gerson Hans Maure ◽  
Muhammad Achmad Chozin ◽  
Edi Santosa

Winged bean (Fabaceae) is a tropical crop that has high nutrient content, and almost all parts of the plant are edible. The research aimed to evaluate the growth and production of winged bean in an intercropping system with tomato. The study was conducted at the Cikabayan Experimental Field of Bogor Agricultural University, Bogor, Indonesia, in a randomized block design with three replications during the rainy season of December 2017 to June 2018. Winged beans were planted in high and low population densities and intercropped with three tomato genotypes, “Tora”, F70030081-12-16-3 and “Apel Belgia”. Monoculture winged bean was assigned as control. Tomatoes were planted between the rows of the winged beans in the intercropping treatment. The results showed that the growth of winged bean in monoculture and intercropping systems was not significantly different. The photosynthetic activity of winged bean at three to five weeks after planting remained high, indicating that the vegetative growth was optimal in both systems. Intercropping increased the winged bean number of pods per plant by 12.66-19.52% compared to monoculture, irrespective of population density. Therefore, winged bean could be considered as suitable to grow in intercropping systems.


2021 ◽  
Vol 11 (1) ◽  
pp. 72-83
Author(s):  
Ni Komang Hita Wulandari ◽  
Kadek Rahayu Puspadewi ◽  
Putu Ledyari Noviyanti

This correlational research aims to determine the relationship between emotional intelligence, independent learning and learning creativity with the mathematics learning outcomes of grade VII students of SMP Negeri 1 Blahbatuh. Data collection was carried out by means of questionnaires and tests. The sample used is a number of 80 people who are determined by the cluter random sampling technique. Hypothesis test used is simple and multiple regression with a significance level of 0.05. The results of this study indicate that there is a significant relationship between emotional intelligence and mathematics learning outcomes, the relative contribution is 46%. There is a significant relationship between independent learning and mathematics learning outcomes, the relative contribution is 39%. There is a significant relationship between creativity and mathematics learning outcomes, the relative contribution is 64%. There is an influence between emotional intelligence, independent learning and learning creativity with mathematics learning outcomes of students with a relative contribution of 62%.


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