scholarly journals Antidiabetic Potential of Methanol Extract of Flamboyant (Delonix regia) Flowers

2021 ◽  
Vol 13 (2) ◽  
pp. 185-194
Author(s):  
Kartini Eriani ◽  
Uswatun Hasanah ◽  
Rizky Fitriana ◽  
Widya Sari ◽  
Yunita Yunita ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Methanol Extract ◽  
Negative Control ◽  
Male Rats ◽  
Glucose Measurement ◽  
Optimum Dose ◽  
Cell Degeneration ◽  
Pancreatic Cell ◽  
Delonix Regia ◽  
Flower Extract

Providing scientific evidences for the medicinal benefits and possible toxic effects of the flamboyant flowers (Delonix regia (Bojer ex Hook.) Raf.) are very important to implementing the medicinal plant in this modern era. This study aimed to investigate antidiabetic potential of methanol extract of flamboyant flowers using a completely random design. Twenty-four male rats were randomly divided into 6 treatment groups with 4 replications: healthy rats (normal control, P1), diabetic rats treated with flamboyant flower extract of 0 (negative control, P2), 100 (P3), 200 (P4), and 400 mg/kg BW (P5), and rats which were induced by 0.45 mg/kg BW of Glibenclamide (positive control, P6). Diabetic condition was achieved by a single injection of alloxan 150 mg/kg BW. Treatments were given once a day for 14 days. On day 0, 3 and 18 blood samples were withdrawn from rats’ orbital vein for glucose measurement. All rats were sacrificed for liver, gastrocnemius muscles and pancreatic tissues collection. The liver and gastrocnemius muscle were subjected for glycogen measurement whereas pancreatic tissues were processed for histological examinations. Data was analyzed by ANOVA and followed by Duncan test. The results showed that flamboyant flowers extracts significantly (p<0.05) reduced blood glucose as well as degeneration and necrosis of pancreatic β cells. Optimal dose to decrease blood glucose and pancreatic cell degeneration was 200 mg/kg BW; whereas optimum dose to decrease pancreatic cell necrosis was 400 mg/kg BW. In conclusion, flamboyant flower extract can reduce blood glucose in rats. This is the first that shows antidiabetic potential of local Flamboyant flower extracts along its toxicity effect to pancreatic tissues. These information could become a basic consideration for the use of the plant extracts as a candidate to cure patients with diabetic problems.

scholarly journals Neurogenic Differentiation of Bone Marrow Mesenchymal-Like Stem Cell Induced by Delonix regia Flowers Extract

2018 ◽  
Vol 10 (2) ◽  
pp. 417-423
Author(s):  
Kartini Eriani ◽  
Irma Suryani ◽  
Al Azhar ◽  
Risa Nursanti ◽  
Ichsan Ichsan ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Bone Marrow Cells ◽  
Population Doubling ◽  
Optimum Dose ◽  
Population Doubling Time ◽  
Neurogenic Differentiation ◽  
Delonix Regia ◽  
Flower Extract ◽  
And Control

Stem cell technology has great potential in the effort to cure degenerative diseases. This study was done to determine optimum dose of flamboyant (Delonix regia) flower extract to induce proliferation and differentiation of mice (Mus musculus) bone marrow mesenchymal-like stem cell. Bone marrow cells were collected from mice by aspiration. Cells suspension (1 x 106) were poured into petri dishes containing 2 ml of modified Dulbecco's Modified Eagle's Media (mDMEM) and incubated overnight at 37 °C in a 5% CO2 incubator and microscopically observed. In quadriplicate, MSC were cultured in mDMEM containing D. regia flower extract of 0.0 (control), 0.4, 0.6, 0.8, and 1.0 mg/ml and incubated at 37 °C for 9 days. Population doubling time (PDT) and differentiated cell type were microscopically observed using HE staining on day 1 and 10. Data obtained were analyzed by ANOVA and Tukey test. The results showed that the addition of D regia flowers extracts 0.8 and 1.0 mg/ml significantly reduced PDT compared to that of 0.4, 0.6 and control. The extract, at 0.4 and 0.6 mg/ml, were able to induce MSC differentiation into fibroblast-like and nerve-like cells. In conclusion, D. regia flower extracts of 0.6, 0.8 and 1.0 mg/ml were able to stimulate MSC proliferation, but optimum dose for neurogenic differentiation was 0.6 mg/ml. This is the first to show potential of D. regia flower extract as neurogenic differentiatian inducer on mice MSC. These findings can be used as preliminary information for using the extract as cellular differentian inducer in basic and applicative reseach using stem cells.

scholarly journals Pengaruh suplementasi β-carotene terhadap kadar glukosa darah dan kadar malondialdehida pada tikus sprague dawley yang diinduksi Streptozotocin

2014 ◽  
Vol 2 (2) ◽  
pp. 41-46
Author(s):  
Elida Soviana ◽  
Banundari Rachmawati ◽  
Nyoman Suci Widyastiti
Keyword(s):  
Free Radicals ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Negative Control ◽  
Male Rats ◽  
Sprague Dawley ◽  
Significant Difference ◽  
Decrease Blood Glucose ◽  
Β Carotene

Background : Hyperglycemia on diabetes mellitus can cause increasing of free radicals production. Free radicals caused lipid peroxidation reaction by forming malondialdehyde (MDA). β-carotene has antioxidant activity may inhibit the formation of ROS.Objective : To prove the effect of multilevel doses β-carotene 1 mg/kg BW, 20 mg/kg BW and 20 mg/kg BW on alternate days within 30 days orally supplementation on blood glucose level and MDA level on Sprague Dawley male rats induced by streptozotocin (STZ). Methods : Thirty rats were randomly divided into 5 groups: X1=Negative control/diabetic, X2 (STZ 40 mg/kg BW + BC 1 mg/kg BW), X3 (STZ 40 mg/kg BW + BC 10mg/kg BW), X4 (STZ 40 mg/kg BW + BC 20 mg/kg BW), X5 (technic control/non diabetic). β-Carotene supplementation was given by nasogastric tube on alternate days within thirty days. Blood glucose level was measured by GOD-PAP and MDA level by ELISA with TBARS methods. Data was analized using paired t-test, wilcoxon, one way anova and post hoc bonferroni. Results : there was a significant difference of blood glucose level (p = 0,0001) and MDA level (p = 0,0001) after suplementation β-carotene on alternate days within 30 days orally. β-carotene 10 mg/kg BW was the most effective and efficient dose to lowering blood glucose, while 20 mg/kg BW to lowering MDA level. Conclusion : The multilevel doses β-carotene (1 mg/kg BW, 10 mg/kg BW and 20 mg/kg BW) on alternate days within 30 days orally supplementation can decrease blood glucose and MDA level. β-carotene 10 mg/kg BW is the most effecetive and efficient to decrease blood glucose and β-carotene 20 mg/kg BW to decrease MDA level.

scholarly journals Skrining Fitokimia dan Potensi Antidiabetes Ekstrak Etanol Herba Ciplukan (Physalis Angulata L) pada Tikus Putih (Rattus Novergicus) yang Diinduksi Aloksan

2019 ◽  
Vol 4 (3) ◽  
pp. 98-107
Author(s):  
Hendra Pratama Maliangkay ◽  
Rolef Rumondor ◽  
Mynia Kantohe
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Negative Control ◽  
Glucose Measurement ◽  
Percentage Decrease ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Physalis Angulata

The purpose of this study to determine the anti-diabetic potential of ciplukan herbal ethanol extract (Physalis angulata L) in white rats (Rattus norvegicus) induced alloxan given intraperitoneally and comparing its effectiveness with glibenclamide. This study uses 5 groups namely giving aquadest as normal control, giving alloxan as a negative control, giving alloxan and glibenclamide as a positive control and the ethanol extract of ciplukan herbal with a dose of 150 mg/kg BB and 300 mg/kg BB. Blood glucose measurement performed on day 0 (three days after induction), day 7, and day 14. The results showed that ethanol extract of ciplukan herbal (Physalis angulata L) can lower blood glucose levekls. A dose of 150 mg/kg BB have percentage decrease in blood glucose levels on day 7 and day 14 respectively by 14.1%and 70.5%. dose of 300 mg/kg BB have a percentage decrease in blood glucose levels on day 7 and day 14 respectively by 70.5%.and 69.5%. a dose of 150 mg/kg BB is more effective as an anti-diabetic compared with a dose of 300 mg/kg BB. Ethanol extract of ciplukan herbal (Physalis angulata L) also has an activity to repair damaged pancreatic-β cells in rats (Rattus norvegicus) induced by alloxan.

scholarly journals The Antidiabetic Activity ofNigella sativaand Propolis on Streptozotocin-Induced Diabetes and Diabetic Nephropathy in Male Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Haddad A. El Rabey ◽  
Madeha N. Al-Seeni ◽  
Amal S. Bakhashwain
Keyword(s):  
Diabetic Nephropathy ◽  
Methanol Extract ◽  
Nigella Sativa ◽  
Lipid Peroxide ◽  
Negative Control ◽  
Male Rats ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Standard Diet ◽  
The Third

This study was conducted to compare the ameliorative effect ofNigella sativaand propolis methanol extract onstreptozotocin-induced diabetic male rats and treating diabetic nephropathy. Forty male Albino rats were divided into four groups; the first group was the negative control fed standard diet. The other 30 rats were injected withstreptozotocin to inducediabetes by a single intravenous injection and then divided equally into three groups; the second group was the positive diabetic control; the third and the fourth groups were treated orally with 20% w/wNigella sativaseeds methanol extract and propolis methanol extract (20% w/w), respectively. The rats of the second group showed increased glucose levels and lipid peroxide accompanied with reduction in superoxide dismutase, catalase, and glutathione-S-transferase enzyme activities compared with the negative control. Carboxymethyl lysine, interleukin-6, and immunoglobulins were also increased as a result of diabetes. Kidney function parameters were also elevated, while potassium and sodium levels were decreased. Moreover, tissues of kidney and pancreas showed severe histopathological changes. Treating the diabetic rats withNigella sativaand propolis methanol extract in the third and fourth groups, respectively, ameliorated all altered biochemical and pathological examinations approaching the negative control. Propolis was more effective thanNigella sativa.

scholarly journals Efek Pemberian Biskuit Ulva terhadap Penurunan Kadar Glukosa Darah Tikus yang Diinduksi Sukrosa

2021 ◽  
Vol 16 (1) ◽  
pp. 63
Author(s):  
Ellya Sinurat ◽  
Dina Fransiska ◽  
Sihono Sihono ◽  
Rinta Kusumawati
Keyword(s):  
Blood Glucose ◽  
Dietary Fiber ◽  
Glucose Intolerance ◽  
Negative Control ◽  
Test Method ◽  
Male Rats ◽  
Oral Glucose ◽  
Glucose Levels ◽  
Blood Biochemical ◽  
Blood Glucose Levels

Rumput laut Ulva sp. memiliki kandungan serat pangan tinggi yang diketahui memiliki aktivitas hipoglikemik. Penelitian ini telah melakukan penambahan Ulva sp. pada biskuit sebagai makanan sehat yang kaya serat pangan. Tujuan penelitian untuk mengetahui pengaruh pemberian biskuit Ulva terhadap penurunan kadar glukosa darah pada tikus jantan yang diinduksi sukrosa jenuh. Selain itu, diamati profil hematologi dan biokimia klinis darah sebelum dan setelah pemberian biskuit Ulva. Uji antidiabetes dilakukan menggunakan uji toleransi glukosa oral terhadap tikus jantan yang diinduksi sukrosa jenuh. Biskuit Ulva yang diberikan 1 g/kg berat badan (BB) tikus dengan perlakuan kontrol negatif (pakan tanpa biskuit), biskuit tanpa Ulva sp., dan biskuit Ulva setara dengan Ulva sp. 1, 5, dan 10 mg/kg BB. Perlakuan dosis diberikan pada 5 ekor tikus percobaan sekali sehari selama 14 hari. Pengamatan terhadap intoleransi glukosa dilakukan melalui pengukuran glukosa darah setelah pemberian sukrosa jenuh ke semua perlakuan pada hari ke-14, dan diukur pada menit ke-0, 30, 60, dan 120. Penimbangan tikus dilakukan pada hari ke-0, 7, dan 14, sedangkan analisis hematologi dan biokimia klinis darah dilakukan pada hari ke-0 dan ke-14. Pemberian biskuit Ulva berpengaruh signifikan terhadap kadar glukosa darah, serta menurunkan hematokrit dan hemoglobin darah tikus. Biskuit dengan dosis Ulva setara 1 mg/kg BB mampu menurunkan kadar glukosa darah tikus secara efektif pada menit ke-60. Tidak ada perbedaan kenaikan berat badan tikus jantan antara kelompok kontrol negatif dan biskuit Ulva pada hari ke-0, 7, dan 14. Pemberian biskuit Ulva sampai dengan 10 mg/kg BB tidak mempengaruhi SGOT, SGPT, ureum, dan kreatinin tikus. ABSTRACTSeaweed Ulva sp. contains high dietary fiber which is known to have hypoglycemic activity. In this study, the addition of Ulva sp. in biscuit products as a healthy food rich in dietary fiber. The objective of this study was to determine the effect of Ulva added biscuits on blood glucose levels reduction in male rats induced by saturated sucrose. In addition, clinical hematology and blood biochemical profiles before and after the administration of Ulva biscuits were also observed. Antidiabetic method used the oral glucose intolerance test method on male rats induced by saturated sucrose. Ulva biscuits were given at 1 g/kg body weight of rats for each treatment. This test used five treatments, namely negative control (rats feeding without biscuits), rat feeding without Ulva added biscuits; and rat feeding with Ulva biscuits equivalent to 1, 5, and 10 mg Ulva sp. /kg BW. Each dose treatment was given to five experimental rats once a day for 14 days. Observations on glucose intolerance included measurement of blood glucose levels by giving saturated sucrose to all treatments and measured at 0, 30, 60, and 120 minutes after administration of saturated sucrose. The weighing was carried out on day 0, 7, and 14, while clinical hematological and blood biochemical analyzes were performed on day 0 and 14. The administration of Ulva biscuits had a significant effect on the blood glucose levels of male rats, lowering  hematocrit and hemoglobin in rat blood. The concentration of 0.1% Ulva biscuits in biscuits (equivalent to a dose of Ulva sp. 1 mg/kg BW) was able to effectively reduce the blood glucose levels of rats after 60 minutes. There was no difference in weight gain of male rats between the negative control group and Ulva biscuits on days 0, 7, and 14. The diet of Ulva biscuits with 10 mg/kg BW Ulva sp. did not affect the SGOT, SGPT, urea, and creatinine of rats.

scholarly journals PENGARUH EKSTRAK ETANOLIK BUAH PARIJOTO (Medinilla speciosa Blume) TERHADAP KADAR GULA DARAH DAN FUNGSI SEKSUAL TIKUS JANTAN GALUR WISTAR MODEL DIABETES MELLITUS KRONIK

2018 ◽  
Vol 15 (2) ◽  
pp. 1
Author(s):  
Rina Wijayanti ◽  
Asih Puji Lestari
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Sexual Function ◽  
Glucose Level ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Negative Group ◽  
One Way Anova

ABSTRACTChronic diabetes mellitus is a disease of increased blood glucose levels in a long time so that it can affect sexual function such as damage spermatogenesis and aphrodisiac function. Parijoto has flavonoid content. This study aimed to determine the effect of ethanolic extract of parijoto (Medinilla speciosa Blume) fruit in blood glucose level and the sexual function in alloxan-induced diabetic male rats. The design of the study was post test only control group design, 25 male wistar rats aged 2 months with BB 100-200 gram divided into 5 groups. Group 1 (normal control), group 2 (negative control) induced alloxan 100mg / kgBB, groups 3, 4, and 5 were alloxan induced and administered EEBP at doses of 100 mg / kgBW, 250 mg / kgBW, and 500 mg / kgBW. Alloxan-induced rats were left for 4 weeks to achieve chronic DM. Data analysis of blood glucose level and spermatozoa quality using one way anova followed by LSD post hoc. While aphrodisiac of introducing and climbing using one way anova continued by LSD and coitus using kruskal wallis. The results showed that there were significant differences between negative control with normal group, III, IV, and V. The effect of ethanolic extract of parijoto fruit on blood glucose level and sexual function in alloxan-induced diabetic male rats showed no difference between treatment group of 100 mg / kgBW with negative group, and there were significant differences between treatment group of 250 mg / kgBW and 500 mg / kgBW with negative group. Ethanolic extract of parijoto fruit at dose 250 mg / kgBB and 500 mg / kgBB decrease blood glucose level, improve and increase sexual function in alloxan-induced diabetic male rats.

scholarly journals Uji Potensi Nefropati Diabetes Daun Sirih Merah (Piper croatum Ruiz & Pav) pada Tikus Putih Jantan (Rattus norvegicus)

KOVALEN ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 239-251
Author(s):  
Joni Tandi ◽  
Rizaldy Lalu ◽  
Magfirah ◽  
Yunlis Silintowe Kenta ◽  
Ronaldy Nobertson
Keyword(s):  
Blood Glucose ◽  
White Male ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Average Dose ◽  
Average Value ◽  
Betel Leaf ◽  
White Rats

This study aims to determine the type of secondary metabolite compounds in red betel leaf ethanol extract, the potential of red betel leaf ethanol extract on blood glucose, creatinine, and urea levels of male white rats induced by streptozotocin. This study used 30 white male rats which were divided into 6 groups: normal control, negative control, positive control, the dose of 150, 250, and 350 mg/kg BW. The showed that results ethanol extract of red betel leaves contained alkaloids, flavonoids, phenols, saponins, and tannins. Ethanol extract of red betel leaf at a dose of 150 mg /kg BW is an effective dose in reducing levels glucose blood with an average value of decreased blood glucose levels of 238.25 mg/dL, and has an effect on creatinine and ureum, with an average dose of 350 mg/kg BW with a mean creatinine level of 0.64 mg/dL and mean ureum of 39.68 mg/dL. Keywords: Creatinine, ureum, red betel leaf, streptozotocin

scholarly journals TEST OF THE EFFECTIVENESS OF THE COMBINATION OF CUCUMBER JUICE (Cucumis sativus Linn,) AND LONG BEANS(Vigna sinensis (L.) Savi Ex Has.)ON THE WISTAR MALE RATS THAT IS INDUCED BY SUKROSA

2016 ◽  
Vol 3 (2) ◽  
pp. 29-34
Author(s):  
Yoga Pratama Lumban Tobing ◽  
Robiyanto ◽  
Rafika Sari
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Cucumis Sativus ◽  
Glucose Level ◽  
Positive Control ◽  
Male Rats ◽  
Optimum Dose ◽  
Vigna Sinensis ◽  
Glucose Levels ◽  
Before And After

Diabetes mellitus is metabolic disorder caused by high glucose level due to abnormalities in insulin secretions, work or both that can be caused by alpha-glucosidase and alpha-amylase in intestine. Cucumbers (Cucumis sativus) and cowpeas (Vigna sinensis) are reported as anti-diabetic. The objective of the present study was to determine the effectiveness of cucumber-cowpea juice combination with single juice and metformin on lowering blood glucose level. Rats were made hyperglycemic by induced sucrose 6,825 g/Kg BW orally. Sample was taken on the lateral vein rat tail. Blood glucose levels were observed before and after juice induction in 30, 60, 90, and 120 minutes by using glucometer. The results were analyzed by Mann-Whitney methods. Single cucumber juice 14,11 g/Kg BW and combination cowpea-cucumber juice 26,4 g/Kg BW – 42 g/Kg BW were given the optimum effect. Both the optimum dose have significant level with metformin as positive control (p>0,05). Results showed that the combination have significant level on lowering blood glucose level compared with metformin.

PENGARUH EKSTRAK DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav.) TERHADAP GLUKOSA DARAH MENCIT (Mus musculus L.) JANTAN YANG DIINDUKSI SUKROSA

BioScience ◽  
2018 ◽  
Vol 2 (1) ◽  
pp. 61
Author(s):  
Elsa Yuniarti
Keyword(s):  
Blood Glucose ◽  
Mus Musculus ◽  
Leaf Extract ◽  
Negative Control ◽  
State University ◽  
Betel Leaf ◽  
Randomized Design ◽  
The Subject ◽  
Effective Drugs ◽  
Zoology Department

Patients withdiabetes mellitus (DM) continues to grow because prosperity and people's lifestyles.Treatment of diabetes often usei njections of insulin and oral antidiabetic drugs. Thetreatment has no side effects. Therefore, it is necessary to find effective drugs using plants thatred betel leaf (Piper crocatum Ruiz & Pav.). Red betel leaf contains flavonoids which are antioxidants. This study aims to determine the effect and dose of extract of red betel leaf (Piper crocatum Ruiz & Pav.) The most effective agains blood glucose in mice(Mus musculusL.) male induced sucrose.This study was an experimental study. The research was conducted in October 2015 in the Division of Laboratory Animal and Zoology Department of Biology, State University of Padang. The subject of research in the form of mice (Mus musculus L.) males totaled 24 tails. The design used was completely randomized design (CRD) with 6 treatments and 4 repetitions. The treatment is given as follows: treatment I: the diabetes control without any treatment given, treatment II: as a negative control (sucrose 3 g/kg bw), treatment III: sucrose+suspension of red betel leaf extract (dosage 0,7 g/kg bw), treatment IV: sucrose+suspension of red betel leaf extract (dosage 1,4 g/kg bw), treatment V: sucrose+suspension of red betel leaf extract (dosage of 2,1 g/kg bw) and treatment VI: sucrose+suspension extracts red betel leaf (dosage 2,8 g/kg bw).The results showed that the extract of red betel leaf (Piper crocatum Ruiz & Pav.) at a dose of 0,8 g/kg bw 1,4 g/kg bw 2,1 g/kg bw and 2,8 g/kg bw can lowers blood glucose in mice. However, the most appropriate dose in lowering blood glucose in mice (Mus musculus L.) at 2,8 g/kg bw in mice.

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