scholarly journals Aberrant expression and regulation of NR2F2 and CTNNB1 in uterine fibroids

Reproduction ◽  
2013 ◽  
Vol 146 (2) ◽  
pp. 91-102 ◽  
Author(s):  
Marina Zaitseva ◽  
Sarah J Holdsworth-Carson ◽  
Luke Waldrip ◽  
Julia Nevzorova ◽  
Luciano Martelotto ◽  
...  
Keyword(s):  
Uterine Fibroid ◽  
Molecular Mechanisms ◽  
Primary Cultures ◽  
Uterine Fibroids ◽  
Reproductive Age ◽  
Causal Role ◽  
Aberrant Expression ◽  
Regulatory Pathways ◽  
Shh Pathway ◽  
Common Benign Tumour

Uterine fibroids are the most common benign tumour afflicting women of reproductive age. Despite the large healthcare burden caused by fibroids, there is only limited understanding of the molecular mechanisms that drive fibroid pathophysiology. Although a large number of genes are differentially expressed in fibroids compared with myometrium, it is likely that most of these differences are a consequence of the fibroid presence and are not causal. The aim of this study was to investigate the expression and regulation of NR2F2 and CTNNB1 based on their potential causal role in uterine fibroid pathophysiology. We used real-time quantitative RT-PCR, western blotting and immunohistochemistry to describe the expression of NR2F2 and CTNNB1 in matched human uterine fibroid and myometrial tissues. Primary myometrial and fibroid smooth muscle cell cultures were treated with progesterone and/or retinoic acid (RA) and sonic hedgehog (SHH) conditioned media to investigate regulatory pathways for these proteins. We showed that NR2F2 and CTNNB1 are aberrantly expressed in fibroid tissue compared with matched myometrium, with strong blood vessel-specific localisation. Although the SHH pathway was shown to be active in myometrial and fibroid primary cultures, it did not regulateNR2F2orCTNNB1mRNA expression. However, progesterone and RA combined regulatedNR2F2mRNA, but notCTNNB1, in myometrial but not fibroid primary cultures. In conclusion, we demonstrate aberrant expression and regulation of NR2F2 and CTNNB1 in uterine fibroids compared with normal myometrium, consistent with the hypothesis that these factors may play a causal role uterine fibroid development.

scholarly journals Concept of Granthi with reference to uterine fibroid: An Ayurveda review

2021 ◽  
Vol 9 (02) ◽  
Author(s):  
Jaya Umate ◽  
Soudamini Chaudhari
Keyword(s):  
Life Span ◽  
Uterine Fibroid ◽  
Uterine Fibroids ◽  
Modern Science ◽  
Reproductive Age ◽  
Significant Morbidity ◽  
Reproductive Life ◽  
Reproductive Life Span ◽  
Gynecological Disease ◽  
Reproductive Age Group

Uterine fibroids are the commonest benign tumor of the uterus and also the commonest benign solid tumor in the female. It can cause significant morbidity in women of a reproductive life span. The exact cause of uterine fibroid is unknown. Prevalence of uterine fibroid 5 -20 % of women in the reproductive age group. It can cause significant morbidity in women of a reproductive life span. Not all fibroids cause symptoms. 50% of women are asymptomatic. Fibroids mainly cause symptoms like menorrhagia, dysmenorrhea and cause pressure symptoms on adjacent viscera i. e. bladder, uterus, rectum which affects the everyday activities of women. In Ayurveda all gynecological disease explained under the term yonivyapad. It can be explained as the anatomical and functional abnormalities of the female reproductive system. In Ayurveda granthi can be correlated with the uterine fibroid. In modern science uterine fibroid treats medically, surgically. But it is challenging to establish a satisfactory conservatory medical treatment to date. so in Ayurveda the main purpose is the management of granthi by samprapti vighatan by use of medicine.

scholarly journals Ultrasound assessment of the uterine wall after hysteroscopic myomectomy of submucous uterine fibroids

2020 ◽  
Vol 19 (4) ◽  
pp. 5-21
Author(s):  
I.А. Esipova ◽  
◽  
L.M. Kappusheva ◽  
V.G. Breusenko ◽  
D.M. Lyafisheva ◽  
...  
Keyword(s):  
Uterine Fibroid ◽  
Surgical Navigation ◽  
Uterine Fibroids ◽  
Intraoperative Ultrasound ◽  
Precise Determination ◽  
The State ◽  
Reproductive Age ◽  
New Technique ◽  
Hysteroscopic Myomectomy ◽  
A New Technique

Objective. To study the state of the myometrium and the endometrium after hysteroscopic myomectomy of uterine fibroid using 2D/3D transvaginal echography in patients of reproductive age. Patients and methods. We examined 90 patients of reproductive age with submucous uterine fibroid, who underwent 117 one-, two- or three-step hysteroscopic resections performed with a new technique of transcervical myomectomy. 3D transvaginal echography was performed in all patients with target assessment of the fibroid type and the intended resection site, and also with subsequent assessment of its changes. Results. A new technique of fibroid resection with rigid loops was elaborated. New options for noninvasive 3D-US in preoperative diagnosis of uterine fibroids have been determined. Owing to 3D-US, the state of the endometrium, myometrium at the «resection site» were studied for the first time. Indications for one-, two- and tree-step resections have been specified. Conclusion. 3D transvaginal echography at the stage of planning hysteroscopic myomectomy allows not only a more precise determination of the fibroid type, assessment of its location and size, but also prediction of the length of operative intervention, the likelihood of multi-step resection and a risk of developing postoperative complications, as well as recommendation of other surgical treatments. Intraoperative ultrasound surgical navigation was introduced, which in combination with an appropriate resection technique permits to enhance the safety of surgical procedure. Depending on the echographic image of the endometrium and myometrium at the «resection site», specific recommendations have been formulated on management of the postoperative period that can permit to prevent the formation of synechiae and to determine precise terms of possible pregnancy occurrence. Key words: infertility, hysteroscopic myomectomy, resection site, uterine fibroid, reproductive age, submucosal fibroid, ultrasound examination, 3D-US

scholarly journals Role of Mifepristone in treatment of uterine fibroid: an experience from tertiary care centre in South India

2019 ◽  
Vol 8 (3) ◽  
pp. 974
Author(s):  
S. V. Nachiketha ◽  
Veena Hadi
Keyword(s):  
Side Effects ◽  
Uterine Fibroid ◽  
Tertiary Care ◽  
Reproductive Age ◽  
Tertiary Care Centre ◽  
Common Benign Tumour ◽  
Fibroid Volume ◽  
Reproductive Age Group

Background: Uterine fibroid are the most common benign tumour of the uterus and is seen in 20% of women in their reproductive age group. The aim of the study was to evaluate effect of Mifepristone on uterine fibroid with reference to reduction in size of fibroid and change in symptomatic profile.Methods: It is a hospital based interventional study conducted at KIMS Hubli hospital. 98 patients with symptomatic fibroid uterus were given 50mg of Mifepristone on alternate day for 3 months.Results: Mifepristone treatment significantly reduced mean PBAC score from baseline score of 212.61 to 20.39 at the end of 3rd month of therapy. Mean fibroid volume also reduced significantly from baseline value of 237.95cm3 to 30.45cm3 after 3 months of treatment. At the end of therapy hemoglobin was raised from 9.57g/dl at baseline to 10.42g/dl after 3 months of treatment. No major side effects were observed, and 7% patients had hysterectomy.Conclusions: Mifepristone is very useful option in perimenopausal women with symptomatic fibroid. It reduces fibroid size and its symptoms without any major side effects. However, future long term RCTs are needed to assess the safety and efficacy of Mifepristone.

scholarly journals Fibronectin-Binding Proteins of Staphylococcus aureus Are Involved in Adherence to Human Airway Epithelium

2002 ◽  
Vol 70 (2) ◽  
pp. 620-630 ◽  
Author(s):  
Emmanuel Mongodin ◽  
Odile Bajolet ◽  
Jérôme Cutrona ◽  
Noël Bonnet ◽  
Florence Dupuit ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Airway Epithelium ◽  
Molecular Mechanisms ◽  
Binding Capacity ◽  
Primary Cultures ◽  
Xenograft Model ◽  
Airway Epithelial Cells ◽  
Regulatory Pathways ◽  
Human Airway ◽  
Human Airway Epithelium

ABSTRACT This study was designed to investigate the molecular mechanisms of Staphylococcus aureus adherence to human airway epithelium. Using a humanized bronchial xenograft model in the nude mouse and primary cultures of human airway epithelial cells (HAEC), we showed that S. aureus adhered mainly to undifferentiated HAEC whereas weak adherence (11- to 20-fold lower) to differentiated HAEC was observed (P < 0.01). A fibronectin (FN)-binding protein (FnBP)-deficient strain of S. aureus had a fivefold-lower adherence level to undifferentiated HAEC than did the parental strain (P < 0.005), suggesting that S. aureus FN-binding capacity is involved in the adherence to HAEC. We also showed that 97% of 32 S. aureus clinical strains, isolated from the airway secretions of cystic fibrosis patients (n = 18) and patients with nosocomial pneumonia (n = 14), possessed the two fnb genes. The strains from pneumonia patients had a significantly (P < 0.05) higher FN-binding capacity than did the strains from CF patients. This result was confirmed by the expression of FnBPs, investigated by Western ligand affinity blotting. Our results suggest a major role of FnBPs in the colonization of the airways by S. aureus and point to the importance of the adhesin regulatory pathways in the staphylococcal infectious process.

Expression of long non-coding RNAs ROR and MALAT1 in uterine fibroids

2021 ◽  
Vol 20 (4) ◽  
pp. 17-21
Author(s):  
S.A. Levakov ◽  
◽  
G.Ya. Azadova ◽  
A.E. Mamedova ◽  
Kh.R. Movtaeva ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Uterine Fibroids ◽  
Reproductive Age ◽  
Expression Level ◽  
Control Group ◽  
Tissue Samples ◽  
Non Coding Rna ◽  
Non Coding Rnas ◽  
Long Non Coding Rna

Objective. To study the expression level of long non-coding RNAs ROR and MALAT1 in tissue samples of uterine fibroids. Patients and methods. Samples of myomatous nodes and tissues of normal myometrium in 28 women of reproductive age were examined. The analysis of the expression of long non-coding RNAs was carried out using a real-time reverse-transcription polymerase chain reaction (RT-PCR) with specific primers. Results. There was a significant decrease in the expression level of long non-coding RNA ROR and an increase in the MALAT1 expression in tissue samples of uterine fibroids relative to the control group. Conclusion. The results obtained demonstrate a possible role of long non-coding RNAs in the development of uterine fibroids and correlate with the data which we obtained for patients with endometriosis. Detecting the expression level of long non-coding RNAs can improve the existing methods for diagnosing this disease. However, further research is required to determine the clinical significance of MALAT1 and ROR, and the molecular mechanisms underlying the action of these RNAs in uterine fibroid cells. Key words: long non-coding RNAs, uterine fibroids, myomectomy, lncROR, MALAT1

scholarly journals Herbal Medicine in Uterine Fibroid

2020 ◽  
Author(s):  
Zi-Lin Li ◽  
Tung-Yung Huang ◽  
Yih Ho ◽  
Ya-Jung Shih ◽  
Yi-Ru Chen ◽  
...  
Keyword(s):  
Uterine Fibroid ◽  
Antitumor Agents ◽  
Treatment Options ◽  
Uterine Fibroids ◽  
Herbal Medicines ◽  
Reproductive Age ◽  
Common Benign Tumor ◽  
Life Issues ◽  
Clinically Significant

Uterine fibroids, also known as uterine leiomyoma is the most common benign tumor of the uterus found in women of reproductive age. Uterine fibroids are the cause of major quality-of-life issues for approximately 25% of all women who suffer from clinically significant symptoms of uterine fibroid. Despite the prevalence of fibroid, currently, there are no effective treatment options for fibroid. The lack of understanding of the etiology of fibroid contributes to the scarcity of medical therapies available. Sex steroid hormones, dysregulation of cell signaling pathways, miRNA expression, and cytogenetic abnormalities may all implicate in fibroid etiology. Several herbal medicines have been used as anti-inflammation and antitumor agents. All of them have a common capability to inhibit expression of pro-inflammatory cytokines, proliferative genes, and pro-angiogenetic genes. Exploring herbal medicines as remedies lighten the hope of treatment. In the current review article, we discuss signal transduction pathways activated herbal medicines. We also address the possibility of using herbal medicines for uterine fibroid treatment.

scholarly journals Predictors and symptomatic burden of uterine fibroids among women in South-Eastern India: a cross-sectional survey analysis

2019 ◽  
Vol 8 (2) ◽  
pp. 524
Author(s):  
Dharitri Swain ◽  
Chanchal Yadav ◽  
Jyoti Kumari ◽  
Monika Rani ◽  
Priyanka Daunrai Rongmei ◽  
...  
Keyword(s):  
Risk Factors ◽  
Uterine Fibroid ◽  
Uterine Fibroids ◽  
Overweight And Obesity ◽  
Reproductive Age ◽  
Age Group ◽  
Women Of Reproductive Age ◽  
Cross Sectional ◽  
Associated Risk Factors ◽  
Reproductive Age Group

Background: Uterine fibroid is the most common pelvic tumor in women of reproductive age and are asymptomatic in at least 50% of affected women. Various risk factors are associated with development of uterine fibroids during this reproductive age. The present study established various associated risk factors increases the prevalence of uterine fibroid among reproductive age group and clinical symptoms burden of diagnosed case.Methods: A cross sectional study design was used to collect samples for a period of six months in an OPD basis. Purposive sampling technique was used to select the 362 sample of reproductive age group (15-45 years) women in a tertiary care hospital, Southeastern India. Women diagnosed with uterine fibroid by ultrasonography were included as cases. Symptomatic features and associated risk factors of UF were collected through structure interview schedule.Results: Point prevalence of uterine fibroid among women in reproductive age group during the period of six months was 20%. Majority of them were in the age group of 30-39 years. Demographic factor such as overweight and obesity and consume dairy products daily has increased the prevalence of UF, whereas use of oral contraceptive pills and normal BMI had inverse relationship with UF risk. Most of the cases reported of having menstrual disturbances like heavy bleeding, passes blood clots during menstruation, prolonged period, urinary symptoms and pressure symptoms were considered independent predicting factors for the occurrence of uterine fibroid.Conclusions: Uterine fibroid is more prevalent among women of reproductive age causing various bleeding and renal symptoms that can have negative impact on quality of women’s life.

scholarly journals Anatomical location of uterine fibroids among women attending a tertiary health facility in a southern Nigerian population

2021 ◽  
Vol 14 (2) ◽  
pp. 007-015
Author(s):  
Joy E. Olotu ◽  
Michael Okon
Keyword(s):  
Uterine Fibroid ◽  
Uterine Fibroids ◽  
Anterior Wall ◽  
Point Prevalence ◽  
Reproductive Age ◽  
Radiology Department ◽  
Anatomical Location ◽  
Ultrasound Scan ◽  
Nulliparous Women ◽  
Study Population

Uterine fibroids are benign monoclonal tumours of the smooth muscle cells of the uterus and usually occur during reproductive age. Studies have revealed that it is more prone to women of African descent and the major factors responsible for this occurrence is yet to be accurately discovered. The study determined prevalence, sonographic location, and type of uterine fibroid among women undergoing ultrasound scan in Radiology Department of the University of Port Harcourt Teaching Hospital. The research was a retrospective study conducted from August to October 2019. Study population was 271 and included all consenting females within ages 18-55 years undergoing ultrasound scan at the study centre. Primary data was collected by direct interview using data collection sheet, parameters retrieved included age, marital status, parity and history of previous surgery, while secondary data collected from the Unit register and We extracted information about, presence, number and location of the fibroids from participant’ medical records. This was done for only women with confirmed cases of uterine fibroids. Analysis of data was done using SPSS version 22 and presented in diagrams, tables, figures and charts where appropriate. Point Prevalence was found to be 33.9%, respondents within ages 31 to 35 years (26.4%) presented with the highest prevalence of fibroid. Multiple fibroids with two or more nodules were found to be the most common from ultrasound scan (64.8%). In terms of types, intramural had the highest occurrence at 61.9%. Tumours are most frequently located in the anterior wall of the uterus (14.4%). Type and location of fibroid was seen to be mostly intramural and on the anterior wall of the uterus, respectively. This study found prevalence of uterine fibroid to be 33.9%, also nulliparous women were found to have the highest incidence of uterine fibroids. The point prevalence value for the study population is high, therefore, we suggest that awareness campaign be increased in the region

scholarly journals Peranan Resveratrol terhadap Progresivitas Uterine Fibroid

2021 ◽  
Vol 2 (2) ◽  
pp. 102-109
Author(s):  
Made Indira Dianti Sanjiwani ◽  
I Made Widianantara
Keyword(s):  
Uterine Fibroid ◽  
Genetic Factors ◽  
Uterine Fibroids ◽  
Reproductive Age ◽  
Women Of Reproductive Age ◽  
Matrix Deposition ◽  
Grape Skins ◽  
Extracellular Matrix Deposition ◽  
Over 40 Years

Uterine fibroids or leiomyomas are benign neoplasms that occur in the uterus and still become a health problem among women of reproductive age. Predominantly, uterine fibroids are found at productive age but some cases are found after menopause. Based on previous studies, there were 145 cases of uterine fibroids in 2014 and 69.7% were found in women over 40 years. Patients with uterine fibroids show a decreased quality of life with symptoms such as heavy uterine bleeding, pelvic pain, and loss of pregnancy. The pathogenesis of uterine fibroids is still unknown, but genetic factors, cytokines, growth factors, and steroid hormones are weak and play an important role in the development of uterine fibroids. Several studies have shown the effect of resveratrol that can be obtained from grape skins. Resveratrol can reduce extracellular matrix deposition and uterine fibroid cell proliferation. Looking at the induced events then resveratrol is effective in preventing the progression of uterine fibroids.

scholarly journals Venous Thromboembolism in Women with Uterine Fibroids

Blood ◽  
2020 ◽  
Vol 136 (Supplement 1) ◽  
pp. 28-29
Author(s):  
Hira Latif ◽  
Valentina Baez Sosa ◽  
Saira Farid ◽  
Stephen Fernandez ◽  
Nicholas Hazen ◽  
...  
Keyword(s):  
Platelet Count ◽  
Venous Thromboembolism ◽  
Uterine Fibroid ◽  
Tertiary Care ◽  
Uterine Fibroids ◽  
Reproductive Age ◽  
Hypercoagulable State ◽  
Morbidly Obese ◽  
Average Weight ◽  
Increased Risk

Background:Uterine fibroids, also called leiomyomas, are diagnosed in over two-third of females of reproductive age; about a quarter warrant treatment. Venous thromboembolism (VTE) is a challenging complication in patients with fibroids due to the increased risk of bleeding with anticoagulation, especially in the setting of associated menorrhagia. The incidence of deep vein thrombosis (DVT) is increased with higher uterine weight which may be related to the extrinsic venous compression of the iliac veins or the inferior vena cava. However, there is reported discordance between the site of uterine fibroid and the site of the DVT along with pulmonary embolism (PE) without evidence of lower extremity DVT suggesting an underlying hypercoagulable state that is more complex than mere hormonal and mechanical factors. Data regarding VTE in patients with uterine fibroids in the US is sparse and is limited to case reports. We performed a retrospective case control study to describe the rate and associated risk factors of VTE in patients with uterine fibroids. Objectives:Evaluate the incidence of VTE events in patients with uterine fibroids and explore trends and associations. Methods:A retrospective chart review of patients at our single, academic, DC metropolitan hospital was conducted. Patients were identified using electronic medical records via ICD-10 codes. Those seen as an inpatient or outpatient in our tertiary care fibroid center with a new diagnosis of uterine fibroids between January 1, 2015, and December 31, 2019 were included and compared to age matched controls of patients with uterine fibroids without VTE in a 1:2 ratio. Statistical analysis was reported as means and medians for descriptive data. Pearson's correlation was performed to determine associations. Results:A total of 6095 patients were diagnosed with uterine fibroids in 5 years, of which 2.2% (n=139) were found to have VTE after the diagnosis of uterine fibroid was made (see table 1). Of the patients with VTE, 88 had DVTs,63 had PE and 12 developed both DVT and PE. The mean age at diagnosis of VTE was 54 (SD=14.95) years. The majority of the patients in the VTE group were African Americans (86%) and mean BMI was 34.52 (SD =9.53), of which about 2/3 were obese or morbidly obese. Average weight of fibroid(s) for those who underwent surgical resection who had available records was noted to be 321 grams (n=11). Data regarding anticoagulation was available for 78% of the patients; the anticoagulant of choice being enoxaparin in 59%, warfarin in 9% and DOACs in 10% of the patients. Two Hundred and thirty-six age matched controls were identified. Statistically significant associations with BMI, race and comorbidities (including atrial fibrillation, congestive heart failure, diabetes, hypertension, HIV, pregnancy and cancer) were found in cases when compared to the control population. Furthermore, cases were found to have a lower mean hemoglobin (10.4 mg/dL) and higher platelet count (311,000/microL) which were statistically significant (p=0.0003). The use of non-steroidal anti-inflammatory drugs and iron supplementation was significantly more in cases than controls. D-dimer and ferritin levels seemed to trend higher and iron saturation lower in cases than controls but were not found to be statistically significant. Conclusions:VTE was found in 2.2% of patients with uterine fibroids, which is higher than the reported VTE incidence of 1-2 per 1000 in the general population. A significant association was found with race, which is consistent with literature that suggests African American patients have a higher rate of incident VTE and also comorbid conditions. Women with low hemoglobin and higher platelet count were more likely to experience VTE. The next steps include understanding more associations, laboratory and clinical, that will assist in risk stratification and tailoring management. Future prospective studies to recognize biomarkers that contribute the hypercoagulable state of uterine fibroids is warranted. Disclosures Morozov: Medtronic:Consultancy;Lumenis:Speakers Bureau;AbbVie:Speakers Bureau.Fitzpatrick:Pfizer:Honoraria.

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