scholarly journals HEPATOPROTECTIVE ACTION OF AQUEOUS EXTRACT OF Artemisia pallens lEAVES IN CLOFIBRATE AND PHENOL TREATED FRESHWATER FISH Pangasius Sp.

2021 ◽  
Vol 14 (2) ◽  
pp. 67-78
Author(s):  
B. Vijaya Geetha ◽  
Keyword(s):  
Oxidative Stress ◽  
Cortisol Level ◽  
Sewage Treatment ◽  
Antioxidant Enzyme Activities ◽  
Oxidative Stress Biomarkers ◽  
Fish Model ◽  
Stress Markers ◽  
Source Determination ◽  
Induced Changes ◽  
Artemisia Pallens

Aim: The key motive was to investigate the toxicological upshot of clofibrate and phenol prescribed under human medicine, having potential in water and sediments contamination via input from sewage treatment plants as active pharmaceutical ingredients’ discharge into the environment had kindled present catastrophic effects upon the aquatic ecosystem. Methods: The present study involved, exposing the fish model, Pangasius sp. after acclimatizing them at a suitable LC50 concentration of selective drugs. Their toxic effects were studied in terms of oxidative stress markers, antioxidant status, and protein damage levels in the occupancies under the bioremediation source, Artemisia pallens and further supported by histopathological and cortisol level studies. Results: The results’ comparison between fish maintained under the bioremediation source, when exposed to clofibrate and phenol resulted in severe oxidative stress (significant *P<0.001, #P<0.001) with significant alterations in antioxidant enzyme activities (significant *P<0.001, #P<0.001), histopathological changes and cortisol levels. In the fish exposed to clofibrate and phenol, the significant increase in cortisol level (significant *P<0.05, #P<0.05) may confer distinctive effects on the cell survival by protecting against oxidative stress-induced changes. Conclusion: Since these results varied with the dwelling of the bioremediation source, determination of oxidative stress biomarkers in Pangasius along with Artemisia pallens may serve as a convenient approach for pollution biomonitoring.

Salt tolerance of the halophyte Limonium delicatulum is more associated with antioxidant enzyme activities than phenolic compounds

2016 ◽  
Vol 43 (7) ◽  
pp. 607 ◽  
Author(s):  
Souid Aymen ◽  
Gabriele Morena ◽  
Longo Vincenzo ◽  
Pucci Laura ◽  
Bellani Lorenza ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Photosynthetic Performance ◽  
Polyphenolic Compounds ◽  
Antioxidant Enzyme Activities ◽  
Strongly Correlated ◽  
Growth Responses ◽  
Antioxidant Responses ◽  
Stress Markers ◽  
Biochemical Assays ◽  
Nacl Concentration

In this work we studied the effect of salinity (ranging from 50 to 500 mM NaCl) on the physiological and the antioxidant responses of the local halophyte Limonium delicatulum Kuntze. We based our analysis on 12 biochemical assays that are commonly used to measure the antioxidant responses under stress such as oxidative stress markers, enzymes activities and polyphenolic compounds. Our aim was to study parameters that are strongly correlated with the growth response to salinity. Results showed two different growth responses depending on the concentration of NaCl in the medium. Under 50 to 200 mM, the growth was stimulated before it decreased significantly at 300–500 mM. L. delicatulum revealed a good aptitude to maintain photosynthetic machinery by increasing the concentrations of photosynthetic pigments, which is essential for the stabilisation of photosystems and the photosynthesis process under optimal NaCl concentration. Their breakdown at higher salinity decreased the photosynthetic performance of plants resulting in growth inhibition. Moreover, to reduce the damaging effect of oxidative stress and to tolerate the accumulation of salt ions, L. delicatulum induced the activities of their antioxidant enzymes more than their contents in polyphenolic compounds.

scholarly journals Reversal of age-associated oxidative stress in mice by PFT, a novel kefir product

2020 ◽  
Vol 34 ◽  
pp. 205873842095014
Author(s):  
Mamdooh Ghoneum ◽  
Shaymaa Abdulmalek ◽  
Deyu Pan
Keyword(s):  
Oxidative Stress ◽  
Low Density Lipoprotein ◽  
Redox Homeostasis ◽  
Density Lipoprotein ◽  
Antioxidant Enzyme Activities ◽  
Protective Effects ◽  
Oxidative Stress Biomarkers ◽  
Age Related ◽  
Total Antioxidant ◽  
The Brain

Introduction: Oxidative stress is a key contributor to aging and age-related diseases. In the present study, we examine the protective effects of PFT, a novel kefir product, against age-associated oxidative stress using aged (10-month-old) mice. Methods: Mice were treated with PFT orally at a daily dose of 2 mg/kg body weight over 6 weeks, and antioxidant status, protein oxidation, and lipid peroxidation were studied in the brain, liver, and blood. Results: PFT supplementation significantly reduced the oxidative stress biomarkers malondialdehyde (MDA) and nitric oxide; reversed the reductions in glutathione (GSH) levels, total antioxidant capacity (TAC), and anti-hydroxyl radical (AHR) content; enhanced the antioxidant enzyme activities of glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD); inhibited the liver enzyme levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT); significantly reduced triglyceride (TG), total cholesterol (TC), and low density lipoprotein (LDL) levels; and significantly elevated high density lipoprotein (HDL) levels. Interestingly, PFT supplementation reversed the oxidative changes associated with aging, thus bringing levels to within the limits of the young control mice in the brain, liver, and blood. We also note that PFT affects the redox homeostasis of young mice and that it is corrected post-treatment with PFT. Conclusion: Our findings show the effectiveness of dietary PFT supplementation in modulating age-associated oxidative stress in mice and motivate further studies of PFT’s effects in reducing age-associated disorders where free radicals and oxidative stress are the major cause.

scholarly journals Protective Role of Probiotic Supplements in Hepatic Steatosis: A Rat Model Study

2020 ◽  
Vol 2020 ◽  
pp. 1-15 ◽  
Author(s):  
Aein Azarang ◽  
Omid Farshad ◽  
Mohammad Mehdi Ommati ◽  
Akram Jamshidzadeh ◽  
Reza Heidari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepatic Steatosis ◽  
Bacillus Coagulans ◽  
Male Rats ◽  
Health Priorities ◽  
Oxidative Stress Markers ◽  
Oxidative Stress Biomarkers ◽  
Nonalcoholic Fatty Liver ◽  
Stress Markers ◽  
And Oxidative Stress

Background. Treating nonalcoholic fatty liver disease (NAFLD) is considered one of the public health priorities in the past decade. So far, probiotics have represented promising results in controlling the signs and symptoms of NAFLD. However, attempts to find the ideal probiotic strain are still ongoing. The present study is designed to find the best strain amongst suitable probiotic strains according to their ability to ameliorate histopathological and oxidative stress biomarkers in hepatic steatosis-induced rats. Methods. Initially, four probiotics species, including Lactobacillus (L.) acidophilus, L. casei, L. reuteri, and Bacillus coagulans, were cultured and prepared as a lyophilized powder for animals. The experiment lasted for fifty days. Initially, hepatic steatosis was induced by excessive ingestion of D-fructose in rats for eight weeks, followed by eight weeks of administering probiotics and D-fructose concurrently. Forty-two six-week-old male rats were alienated to different groups and were supplemented with different probiotics ( 1 ∗ 10 9   CFU in 500 mL drinking water). After eight weeks, blood and liver samples were taken for further evaluation, and plasma and oxidative stress markers corresponding to liver injuries were examined. Results. Administration of probiotics over eight weeks reversed hepatic and blood triglyceride concentration and blood glucose levels. Also, probiotics significantly suppressed markers of oxidative stress in the liver tissue. Conclusions. Although some of the single probiotic formulations were able to mitigate oxidative stress markers, mixtures of probiotics significantly ameliorated more symptoms in the NAFLD animals. This enhanced effect might be due to probiotics’ cumulative potential to maintain oxidative stress and deliver improved lipid profiles, liver function markers, and inflammatory markers.

scholarly journals Physiochemical Studies of Sodium Chloride on Mungbean (Vigna radiataL. Wilczek) and Its Possible Recovery with Spermine and Gibberellic Acid

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Srijita Ghosh ◽  
Sanglap Mitra ◽  
Atreyee Paul
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Gibberellic Acid ◽  
Chlorophyll Content ◽  
Biomass Production ◽  
Oxidative Stress Marker ◽  
Antioxidant Enzyme Activities ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Low Concentrations

The physiological and biochemical responses to increasing NaCl concentrations, along with low concentrations of gibberellic acid or spermine, either alone or in their combination, were studied in mungbean seedlings. In the test seedlings, the root-shoot elongation, biomass production, and the chlorophyll content were significantly decreased with increasing NaCl concentrations. Salt toxicity severely affected activities of different antioxidant enzymes and oxidative stress markers. Activities of antioxidant enzymes, superoxide dismutase (SOD), and catalase (CAT) increased significantly over water control. Similarly, oxidative stress markers such as proline, malondialdehyde (MDA), and hydrogen peroxide (H2O2) contents also increased as a result of progressive increase in salt stress. Combined application of NaCl along with low concentrations of either gibberellic acid (5 µM) or spermine (50 µM) in the test seedlings showed significant alterations, that is, drastic increase in seedling elongation, increased biomass production, increased chlorophyll content, and significant lowering in all the antioxidant enzyme activities as well as oxidative stress marker contents in comparison to salt treated test seedlings, leading to better growth and metabolism. Our study shows that low concentrations of either gibberellic acid or spermine will be able to overcome the toxic effects of NaCl stress in mungbean seedlings.

Beyond lowering circulating LDL: Apheresis-induced changes of systemic oxidative stress markers by four different techniques

2009 ◽  
Vol 10 (5) ◽  
pp. 34-38 ◽  
Author(s):  
Steffi Kopprasch ◽  
Juergen Graessler ◽  
Stefan R. Bornstein ◽  
Peter E.H. Schwarz ◽  
Sergey Tselmin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Stress Markers ◽  
Ldl Apheresis ◽  
Stress Markers ◽  
Systemic Oxidative Stress ◽  
Induced Changes

scholarly journals Association between Vitamin D Supplements, Oxidative Stress Biomarkers, and Hyperbaric Therapy in Patients with Sudden Sensorineural Hearing Loss

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Jarosław Paprocki ◽  
Paweł Sutkowy ◽  
Jacek Piechocki ◽  
Alina Woźniak
Keyword(s):  
Oxidative Stress ◽  
Vitamin D ◽  
Hearing Loss ◽  
Sensorineural Hearing Loss ◽  
Thiobarbituric Acid ◽  
Vitamin D Supplementation ◽  
Sudden Sensorineural Hearing Loss ◽  
Sensorineural Hearing ◽  
Oxidative Stress Biomarkers ◽  
Stress Markers

The effect of vitamin D supplementation to patients with sudden sensorineural hearing loss (SSNHL), treated with hyperbaric oxygen (HBO) therapy, on the markers of the oxidant-antioxidant equilibrium was investigated. Patients were divided into two groups: those who did and did not receive vitamin D (cholecalciferol at 4000 IU/24 h). Concentrations of the following compounds, thiobarbituric acid reactive substances (TBARS), malondialdehyde (MDA), conjugated dienes (CD) in plasma and erythrocytes and activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) in erythrocytes, were determined. Haemoglobin (HGB) and haematocrit (HCT) were measured. Blood for analyses was collected from the basilic vein at three time points: before the first HBO procedure, up to 5 min after the first procedure, and after 14 procedures. No statistically significant differences in parameters tested were found between patients who did and did not receive vitamin D. In patients without supplementation, an increase of 53.2% ( P ≤ 0.05 ) in erythrocyte TBARS was observed after the first HBO treatment. In patients receiving vitamin D, a reduction of 27.6% ( P ≤ 0.05 ) was observed in erythrocyte MDA after 14 HBO treatments vs. that after the first treatment. In both groups, a decrease of 33.3% in plasma CD was observed after 14 treatments vs. that after the first treatment ( P ≤ 0.05 and P ≤ 0.01 , respectively). No statistically significant changes were observed in the erythrocyte SOD, GPx, and CAT activities and in HCT. A reduction of HGB concentration of 10.9% ( P ≤ 0.05 ) was demonstrated in nonsupplemented patients after 14 treatments compared with baseline. The results confirm that the effect of HBO therapy on oxidative stress markers is inconclusive and complex. Repeated HBO procedures can induce adaptive changes which protect against disruption of the oxidant-antioxidant equilibrium. It is possible that vitamin D supplementation inhibits the process of lipid peroxidation in erythrocytes.

scholarly journals From “Every Day” Hormonal to Oxidative Stress Biomarkers in Blood and Follicular Fluid, to Embryo Quality and Pregnancy Success?

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Katarzyna Olszak-Wąsik ◽  
Anna Bednarska-Czerwińska ◽  
Anita Olejek ◽  
Andrzej Tukiendorf
Keyword(s):  
Oxidative Stress ◽  
Follicular Fluid ◽  
Embryo Quality ◽  
R Package ◽  
Single Embryo Transfer ◽  
Oxidative Stress Biomarkers ◽  
Oocyte Growth ◽  
Vitro Fertilization ◽  
Stress Markers

Background. Oxidative stress appears to be involved in oocyte growth and maturation that when impaired results in poor embryo quality and lower potential to implant. The biochemical microenvironment of the oocyte (follicular fluid (FF)) consists of hormones and other various substances regulating the balance between oxidants and antioxidants. Aim. The aim of this study was to examine the possible impact of selected biomarkers (“every day,” hormonal biomarkers, enzymatic and nonenzymatic antioxidants, and also oxidative stress markers) in serum and FF, on embryo quality and pregnancy success in infertile women undergoing infertility treatment. Methods. All 53 patients, mean age 34.7±4.1 years, with serum AMH level≥0.7 ng/mL, were diagnosed with idiopathic infertility. They were stimulated in short antagonist protocol, followed by in vitro fertilization (IVF-ICSI intracytoplasmatic sperm injection) and a single embryo transfer. Follicular fluid was aspirated from the first mature follicle. In statistical analyses the R software was used, then all data was assessed with the Shapiro-Wilk test, logistic regression, and later the receiver operating characteristic (ROC) curve was applied using “pROC” R package. Results. We did not observe any correlation between AMH and embryo quality and pregnancy rate. Statistically significant results were only found for biomarkers examined in follicular fluid. Greater levels of GPX in FF were associated with the increased chance of producing a high quality embryo (the optimal cut-off concentration was established at over 450 lU/L.) Regarding pregnancy success, increasing levels of GR (cut-off at 21 IU/L), CuZnSOD (cut-off at 9NU/mL), and GST (cut-off at 2.5 IU/L) resulted in lower chances of a successful pregnancy. Conclusion. Our results indicate that FF markers may have some advantages in predicting embryo quality and pregnancy over AMH. The GPX system seems to be mostly related to embryo quality and pregnancy.

scholarly journals Oxidative Stress and Antioxidants in Athletes Undertaking Regular Exercise Training

2005 ◽  
Vol 15 (2) ◽  
pp. 131-146 ◽  
Author(s):  
Trent A. Watson ◽  
Lesley K. MacDonald-Wicks ◽  
Manohar L. Garg
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Defenses ◽  
Antioxidant Enzyme Activities ◽  
Regular Exercise ◽  
Activity Levels ◽  
Dietary Intakes ◽  
Stress Markers ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Β Carotene

Exercise has been shown to increase the production of reactive oxygen species to a point that can exceed antioxidant defenses to cause oxidative stress. Dietary intake of antioxidants, physical activity levels, various antioxidants and oxidative stress markers were examined in 20 exercise-trained “athletes” and 20 age- and sex-matched sedentary “controls.” Plasma F2-isoprostanes, antioxidant enzyme activities, and uric acid levels were similar in athletes and sedentary controls. Plasma α-tocopherol and β-carotene were higher in athletes compared with sedentary controls. Total antioxidant capacity tended to be lower in athletes, with a significant difference between male athletes and male controls. Dietary intakes of antioxidants were also similar between groups and well above recommended dietary intakes for Australians. These findings suggest that athletes who consume a diet rich in antioxidants have elevated plasma α-tocopherol and β-carotene that were likely to be brought about by adaptive processes resulting from regular exercise.

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