scholarly journals Biochemical and Physiological Changes in Response to Salinity in Two Durum Wheat (Triticum turgidum L.) Genotypes

2011 ◽  
Vol 39 (1) ◽  
pp. 165 ◽  
Author(s):  
Ezatollah ESFANDIARI ◽  
Vaghef ENAYATI ◽  
Amin ABBASI
Keyword(s):  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Durum Wheat ◽  
Nutrient Solution ◽  
Negative Impact ◽  
Stability Index ◽  
Membrane Stability ◽  
Guaiacol Peroxidase ◽  
Membrane Stability Index ◽  
Significant Difference

The effects of salt stress on the activity of antioxidative enzymes, some oxidative stress indices and Na+ and K+ content were studied in leaves of two durum wheat cultivars, Egypt 449 (salt-tolerant) and Syria 371 (salt-sensitive), grown under control (nutrient solution) or salt stress (nutrient solution containing 200 mM NaCl) conditions. Leaves of control and salt-stressed plants were harvested from 10 days old plants beyond salt treatment. The results showed significant increase for activities of antioxidant enzymes such as ascorbate peroxidase (APX) and guaiacol peroxidase (GPX), in Egypt 449 under salinity. At the same time, in cultivar Egypt 449, activity of SOD and CAT were not changed. Meanwhile, under salinity condition the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and GPX in cultivar Syria 371 was lower than control. There was no significant difference between salinity situation and control ones regarding APX activity. Salt stress elevated the amounts of malondialdehyde (MDA) in both cultivars. However, the increasing rate in Syria 371 was more than (four times) that of Egypt 449. Membrane stability index (MSI) of both cultivars negatively influenced by salinity. This negative impact on Syria 371 was highlighted than on Egypt 449. Hydrogen peroxide (H2O2) content of salinity faced Syria 371 was higher than control. Both cultivars displayed increasing and decreasing trend for Na+ and K+ content, respectively. Moreover, K+/Na+ ratio was decreased in both cultivars due to salinity. The studied parameters elucidated that salt resistance of Egypt 449 might be due to increased activity of antioxidant enzymes, low lipid peroxidation, assumingly lower changes in membrane stability index and avoidance of Na+ absorption.

scholarly journals Antioxidant activity of enzymatic system of two different wheat (Triticum aestivum L.) cultivars growing under salt stress

2011 ◽  
Vol 57 (No. 3) ◽  
pp. 101-107 ◽  
Author(s):  
W.M. Bhutta
Keyword(s):  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Water Content ◽  
Relative Water Content ◽  
Stability Index ◽  
Membrane Stability ◽  
Membrane Stability Index ◽  
Salt Tolerant ◽  
Stress Levels ◽  
Relative Water

Soil salinity and semi-arid and arid climate of Pakistan is a major constraint in agriculture and predominantly in foodstuff production. It limits crop yield and use of land previously uncultivated. Wheat is moderately salt tolerant. A great variation was observed between and within the cultivars (genotypes: S-24 salt tolerant and DN-27 salt sensitive) in relationship to the choice of salinity level (control and treatments: in increment of 25 mol/m<sup>3</sup> NaCl/day to a final level of 80 and 160 mol/m<sup>3 </sup>NaCl into the nutrient solution) that will be used for screening purpose. Relative water content (RWC), membrane stability index and the activities of some antioxidant enzymes were determined after 20 and 40 days of salt stress exposure. As a result of activity enzymes, superoxide dismutase (SOD), peroxidase (POD) and catalase increased in S-24 with the increase of salt stress, while in DN-27 all the enzymes showed constant activity at all the stress levels. Meanwhile, relative water content and membrane stability index decrease the value as well as they increases the stress levels. It can be concluded that all three antioxidant enzymes were limiting factors for these genotypes and these reasons also led to the salt sensitivity in DN-27. Different selection methods should be applied to improve different traits in different conditions in wheat.

scholarly journals Effect of cold stress on boro rice seedlings

2017 ◽  
Vol 9 (2) ◽  
pp. 1036-1041 ◽  
Author(s):  
Priyanka Kumari ◽  
H. K. Jaiswal
Keyword(s):  
Cold Stress ◽  
Water Content ◽  
Chlorophyll Content ◽  
Relative Water Content ◽  
Stability Index ◽  
Membrane Stability ◽  
Membrane Stability Index ◽  
Relative Water ◽  
Cold Tolerant ◽  
Boro Rice

Cold stress at seedling stage is a major constraint in boro rice production. Nine boro rice lines were crossed in diallel fashion excluding reciprocals to obtain 36 crosses. All the 36 crosses along with parents were grown in nursery in three seasons (boro-2014, kharif-2015 and boro-2015). Performance of seedlings for survival per cent, chlorophyll content, relative water content, membrane stability index was recorded just before transplanting in all the three seasons. Scoring for cold tolerance was done in both boro seasons. Gautam showed highest survival rate over three seasons. Among crosses, IR 64 x Krishna Hamsa showed highest survival (84%) in boro-2014, MTU 1010 x Jaya (86.33%) in boro-2015 and MTU 1010 x Krishna Hamsa (95.67%) in kharif-2015. Jaya x Krishna Hamsa was most cold tolerant cross over both boro seasons. Significant positive correlation was observed among survival per cent, chlorophyll content, relative water content and membrane stability index over seasons.

scholarly journals Potential use of germicides in vase solutions for gladiolus ‘White Friendship’

2017 ◽  
Vol 23 (1) ◽  
pp. 79
Author(s):  
Maria Carolina Casares ◽  
Ben-Hur Mattiuz ◽  
Ana Correa Muniz ◽  
Claudia Mattiuz
Keyword(s):  
Water Balance ◽  
Stability Index ◽  
Membrane Stability ◽  
Fresh Mass ◽  
Postharvest Quality ◽  
Flower Senescence ◽  
Vase Life ◽  
Membrane Stability Index ◽  
Ambient Conditions ◽  
Inflorescence Development

Gladiolus (Gladiolus x hortulanus) is one of the most important crops for cut flowers worldwide, however having a short longevity. Usually, stems are harvested at the bud stage and need water and carbohydrates to support inflorescence development and to ensure flower quality. Vase solutions can supply these elements. However, sugars may benefit bacterial proliferation, which eventually can block xylem vessels. Bactericides potentially inhibit microorganism proliferation and new compounds are being tested constantly. This experiment was conducted to evaluate ‘White Friendship’ cut gladiolus performance in vase solutions with sodium dichloroisocyanurate dihydrate (Na-DCC), 8-hydroxyquinoline (8-HQ) or polymeric biguanide (PB). Inflorescences harvested at commercial maturity were placed in distilled water with 4% w/v of sucrose plus the germicides and kept under ambient conditions. Vase life, inflorescence postharvest development, fresh mass, membrane stability index and water relations were evaluated. There were no visual symptoms of toxicity in the treatments. The highest values of vase life, fully-open flowers, fresh mass and water balance were obtained with the Na-DCC treatment. Inflorescences treated with 8-HQ exhibited the highest values of solution uptake and wilting flowers. PB had a limited effect in gladiolus postharvest since the obtained results were similar to the sucrose alone. There was no effect of germicides on membrane stability index and its reduction was due to flower senescence progress only. Vase solution with Na-DCC plus sucrose improved water balance, increased open flowers, reduced wilting of flowers and fresh mass loss. Therefore, vase solutions with sucrose 4% w/v and Na-DCC enhance postharvest quality of cut gladiolus inflorescences.

Evaluation of antioxidant enzymes activity in canola under salt stress

2014 ◽  
Vol 2 (2) ◽  
pp. 88-92 ◽  
Author(s):  
Jafar Abili ◽  
Sajjad Zare
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Arid Regions ◽  
Crop Productivity ◽  
Guaiacol Peroxidase ◽  
Randomized Design ◽  
Salinity Levels ◽  
Completely Randomized Design ◽  
Semi Arid

Salinity is one of the major stresses in arid and semi-arid regions causing adverse effects at physiological, biochemical, and molecular levels, limiting crop productivity. In this research, three canola cultivars (Licord, Talayeh, Zarfam) were compared at 5 salinity levels (control, 50, 100, 150 and 200 mM) for their catalase, guaiacol peroxidase, superoxide dismutase activity,proline and yield in a completely randomized design with 3 replications. In our study, we found that NaCl concentrations greater than 150 and 200 mM caused the irreversible disorders. Increased salt concentrations led to significant changes in the levels of antioxidative enzymes and proline in three canola cultivars. Also, yield rates in three varieties decreased in the presence of NaCl concentrations.

scholarly journals The Effect of Trichoderma citrinoviride Treatment under Salinity Combined to Rhizoctonia solani Infection in Strawberry (Fragaria x ananassa Duch.)

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1589
Author(s):  
Askim Hediye Sekmen Cetinel ◽  
Azime Gokce ◽  
Erhan Erdik ◽  
Barbaros Cetinel ◽  
Nedim Cetinkaya
Keyword(s):  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Rhizoctonia Solani ◽  
Antioxidant Defense ◽  
Negative Impact ◽  
Pathogenic Fungi ◽  
Dry Weight ◽  
Antioxidant Defense System ◽  
Psii Efficiency ◽  
Trichoderma Citrinoviride

Trihoderma citrinoviride protects plants from diseases by functioning as antagonists of many pathogenic fungi or by triggering the antioxidant defense system in plants. In the present study, to uncover the possible alleviative role of Trichoderma against salinity and Rhizoctonia solani infection, strawberry plants were pretreated Trichoderma citrinoviride and then subjected to salinity, R. solani and combined salinity and R. solani. The effect of T. citrinoviride on the alleviation of the effects of salt stress and Rhizoctonia solani infection was investigated by analysing leaf dry weight, PSII efficiency, and the activity of some antioxidant enzymes in the leaves of strawberry plants. T. citrinoviride improved competitive capability against salinity and R. solani infection. It showed 79% inhibition of the growth of pathogen R. solani. T. citrinoviride reduced 63% of the severity of disease in the leaves. Trichoderma pretreatment maximized plant dry weight. The T. citrinoviride-pretreated plants showed higher levels of PSII efficiency (Fv/Fm). Decreased lipid peroxidation and H2O2 accumulation compared to untreated seedlings under salt stress and R. solani infection was observed. Trichoderma-pretreated and –untreated plants respond differently to salt stress and R. solani infection by means of antioxidant defense. As compared to untreated seedlings, treated seedlings showed significantly lower activities of antioxidant enzymes, superoxide dismutase (SOD), peroxidase (POX), cell wall peroxidase (CWPOX) under salt stress and R. solani infection, indicating that treated seedlings might sense lower stress as compared to untreated seedlings. The study reports the effective adaptive strategy and potential of T. citrinoviride in alleviating the negative impact of salt stress and R. solani infection in strawberry.

scholarly journals Effect of Ethylene Antagonist Silver Thiosulphate on the Flower Longevity of Clarkia pulchella Pursh.

2018 ◽  
Vol 26 (1) ◽  
pp. 5-12 ◽  
Author(s):  
Riyaz Ahmad Dar ◽  
Inayatullah Tahir
Keyword(s):  
Antioxidant Enzymes ◽  
Stability Index ◽  
Dry Mass ◽  
Control Group ◽  
Total Phenols ◽  
Flower Longevity ◽  
Membrane Stability Index ◽  
Silver Thiosulphate ◽  
Ethylene Antagonist ◽  
Clarkia Pulchella

Abstract An experiment was conducted to study the effect of different concentrations of silver thiosulphate (STS) on flower longevity of Clarkia pulchella Pursh. The buds were subjected to 0.1, 0.25, 0.5, 0.75 and 1 mM of STS for 1 h pulse treatment. A separate set of flowers kept in distilled water was designated the control group. STS treatment resulted in improved flower longevity besides maintaining higher fresh and dry mass, water content and floral diameter. Conversely, total phenols, lipid peroxidation and lipoxygenase (LOX) activity decreased. The flowers treated with STS showed a significant increase in the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). Amongst various grades used, 0.5 mM STS was found to be most effective in enhancing the flower longevity by 1.5 days. The present study reveals that STS maintains lower LOX activity, thereby increased membrane stability index by improving the activity of antioxidant enzymes.

Effect of hexavalent chromium [Cr(VI)] stress in roots of Cr-tolerant and Cr-sensitive barley cultivars

2012 ◽  
Vol 60 (1) ◽  
pp. 29-36 ◽  
Author(s):  
M. Yildiz ◽  
H. Terzi
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Protein Content ◽  
Dehydrogenase Activity ◽  
Total Soluble Protein ◽  
Guaiacol Peroxidase ◽  
Sod Activity ◽  
Barley Cultivars ◽  
Significant Difference

The effect of different chromium [Cr(VI)] concentrations (0, 75, 150 and 225 μM) on dehydrogenase activity, total soluble protein, proline, malondialdehyde (MDA) and antioxidant enzymes was investigated in the roots of two barley cultivars (Cr-tolerant Zeynelağa and Cr-sensitive Orza-96) in hydroponic experiments. The root dehydrogenase activity and protein content decreased with an increase in the Cr(VI) concentration, but no significant difference was found between the two barley cultivars. Cr(VI) stress increased the contents of proline and MDA in both cultivars, but this effect was more pronounced in Orza-96 than in Zeynelağa. The activities of antioxidant enzymes, including superoxide dismutase (SOD), ascorbate peroxidase (APX) and guaiacol peroxidase (POD), exhibited changes. The SOD activity increased in Zeynelağa and decreased in Orza-96 at 225 μM Cr(VI) compared to their controls. Cr(VI) stress decreased the APX and POD activities. Zeynelağa had greater APX activity than Orza-96 at 150 and 225 μM Cr(VI). However, there was no marked difference in POD activities between the two cultivars. The decrease in root dehydrogenase activity and protein content, the increase in proline and lipid peroxidation, and the alterations in the activities of antioxidant enzymes may be indicative of oxidative stress induced by Cr(VI).

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