scholarly journals Amino acids, bioactive compounds and biological activities of ten species from family Commelinaceae in Thailand

2021 ◽  
Vol 49 (3) ◽  
pp. 12391
Author(s):  
Theeraphan CHUMROENPHAT ◽  
Surapon SAENSOUK
Keyword(s):  
Amino Acids ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Frap Assay ◽  
Few Data

Commelinaceae is used for ethnobotany. However, bioactive compounds information of this family is few data. The aim here was to determine the bioactive compounds and quantify the antioxidant activity from leaves 10 species.  Twenty amino acids were identified using LC/MS/MS. The antioxidant contents were evaluated by the total phenolic and total flavonoid content assays. The individual phenolic acids, flavonoids and vitamin c were identified by HPLC. The antioxidant activities detected were DPPH scavenging and FRAP assay. The total amino acids found in most samples studied ranging from 239 to 1012 µg/g DW. Vitamin C contents were in the range of 23 to 195 mg/100 g DW. Total phenolic and total flavonoid contents ranged from 11.3 to 35.7 mg GAE/g DW and 56.7 to 368.7 mg RE/100 g DW, respectively. All the species studied possessed strong antioxidant properties (DPPH and FRAP). This result can be applied for further development of functional foods or cosmetics.

scholarly journals A Comparison of Nutritional Values, Bioactive Compounds, Amino Acids, and Antioxidant Activities of Alfalfa (Medicago sativa) Plant and Pellet for Use as Beneficial Material Ruminant Feed

2021 ◽  
Vol 18 (5) ◽  
Author(s):  
Supreena SRISAIKHAM
Keyword(s):  
Amino Acids ◽  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Neutral Detergent Fiber ◽  
Total Phenolic ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Nutritional Values

Aqueous extracts of whole alfalfa plants (AP) and alfalfa pellets (APe) were examined for nutritional values, bioactive compounds (total phenolic content (TPC), phenolic compounds (PC), total flavonoids content (TFC), flavonoids compounds (FC), isoflavones), amino acids (AA) and antioxidant activity of AP and APe assessed via a DHHP radical scavenging assay and ferric reducing antioxidant power (FRAP) assay. The potential ingredients in the form of AP and APe were used to evaluate the nutritional values measured by chemical composition, and to investigate bioactive compounds, AA and antioxidant activity to further contribute to use as a feed ingredient or application as a high-value alternative feed supplement in the ruminant diet. Crude protein and tannins were higher in the APe (18.17 and 3.08 %) than the AP (16.21 and 1.80 %). Higher content of organic matter, crude fiber, neutral-detergent fiber, acid-detergent fiber, acid-detergent lignin was observed for AP than APe. The TPC and TFC were more abundant in AP than in APe and AP also showed stronger antioxidant activity via DPPH than APe, with positive correlations of TPC, TFC and DPPH. The highest values of gallic acid in terms of PC and apigenin in terms of FC of APe were 215.30 and 2278.27 µg/g dry weight (DW), respectively, while the highest levels of genistein and daidzein occurred in AP at 104.37 and 130.91 µg/g DW, respectively. The total of essential AA was found to be in vary the range of 9.33 to 16689.47 µg/g DW in different forms of samples, with valine as the highest (5451 µg/g DW) in APe, followed by phenylalanine (4.30 µg/g DW) in AP.

scholarly journals Use of A Hydroalcoholic Extract of Moringa oleifera Leaves for the Green Synthesis of Bismuth Nanoparticles and Evaluation of Their Anti-Microbial and Antioxidant Activities

Materials ◽  
2020 ◽  
Vol 13 (4) ◽  
pp. 876
Author(s):  
Prince Edwin Das ◽  
Amin F. Majdalawieh ◽  
Imad A. Abu-Yousef ◽  
Srinivasan Narasimhan ◽  
Palmiro Poltronieri
Keyword(s):  
Green Synthesis ◽  
Moringa Oleifera ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Hydroalcoholic Extract ◽  
Bismuth Nanoparticles ◽  
Fungal Activity ◽  
Anti Fungal

The employment of plant extracts in the synthesis of metal nanoparticles is a very attractive approach in the field of green synthesis. To benefit from the potential synergy between the biological activities of the Moringa oleifera and metallic bismuth, our study aimed to achieve a green synthesis of phytochemical encapsulated bismuth nanoparticles using a hydroalcoholic extract of M. oleifera leaves. The total phenolic content in the M. oleifera leaves extract used was 23.0 ± 0.3 mg gallic acid equivalent/g of dried M. oleifera leaves powder. The physical properties of the synthesized bismuth nanoparticles were characterized using UV-Vis spectrophotometer, FT-IR spectrometer, TEM, SEM, and XRD. The size of the synthesized bismuth nanoparticles is in the range of 40.4–57.8 nm with amorphous morphology. Using DPPH and phosphomolybdate assays, our findings revealed that the M. oleifera leaves extract and the synthesized bismuth nanoparticles possess antioxidant properties. Using resazurin microtiter assay, we also demonstrate that the M. oleifera leaves extract and the synthesized bismuth nanoparticles exert potent anti-bacterial activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Enterococcus faecalis (estimated MIC values for the extract: 500, 250, 250, and 250 µg/mL; estimated MIC values for the bismuth nanoparticles: 500, 500, 500, and 250 µg/mL, respectively). Similarly, the M. oleifera leaves extract and the synthesized bismuth nanoparticles display relatively stronger anti-fungal activity against Aspergillus niger, Aspergillus flavus, Candida albicans, and Candida glabrata (estimated MIC values for the extract: 62.5, 62.5, 125, and 250 µg/mL; estimated MIC values for the bismuth nanoparticles: 250, 250, 62.5, and 62.5 µg/mL, respectively). Thus, green synthesis of bismuth nanoparticles using M. oleifera leaves extract was successful, showing a positive antioxidant, anti-bacterial, and anti-fungal activity. Therefore, the synthesized bismuth nanoparticles can potentially be employed in the alleviation of symptoms associated with oxidative stress and in the topic treatment of Candida infections.

scholarly journals Limosilactobacillus reuteri Fermented Brown Rice: A Product with Enhanced Bioactive Compounds and Antioxidant Potential

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1077
Author(s):  
Akanksha Tyagi ◽  
Umair Shabbir ◽  
Ramachandran Chelliah ◽  
Eric Banan-Mwine Daliri ◽  
Xiuqin Chen ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Current Knowledge ◽  
Oryza Sativa L ◽  
Antioxidant Properties ◽  
Brown Rice ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Gamma Aminobutyric Acid ◽  
The Impact

Oxidative stress has been postulated to play a role in several diseases, including cardiovascular diseases, diabetes, and stress-related disorders (anxiety/depression). Presently, natural plant-derived phytochemicals are an important tool in reducing metabolomic disorders or for avoiding the side effects of current medicinal therapies. Brown Rice (Oryza sativa L.) is an important part of Asian diets reported as a rich source of bioactive phytonutrients. In our present study, we have analyzed the effect of different lactic acid bacteria (LABs) fermentation on antioxidant properties and in the enhancement of bioactive constituents in Korean brown rice. Therefore, the antioxidant activities and phytochemical analysis were investigated for raw brown rice (BR) and different fermented brown rice (FBR). BR fermented with Limosilactobacillus reuteri, showed the highest antioxidant activities among all samples: DPPH (121.19 ± 1.0), ABTS (145.80 ± 0.99), and FRAP (171.89 ± 0.71) mg Trolox equiv./100 g, dry weight (DW). Total phenolic content (108.86 ± 0.63) mg GAE equiv./100 g, DW and total flavonoids content (86.79 ± 0.83) mg catechin equiv./100 g, DW was also observed highest in Limosilactobacillus reuteri FBR. Furthermore, phytochemical profiling using ultra-high-performance liquid tandem chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) and cell antioxidant assay (CAA) revealed L. reuteri FBR as a strong antioxidant with an abundance of bioactive compounds such as gamma-aminobutyric acid, coumarin, cinnamic acid, butanoic acid, ascorbic acid, nicotinic acid, and stearic acid. This study expanded current knowledge on the impact of fermentation leading to the enhancement of antioxidant capacity with an abundance of health-related bioactive compounds in BR. The results obtained may provide useful information on functional food production using fermented brown rice.

scholarly journals Total Vitamin C, Ascorbic Acid, Dehydroascorbic Acid, Antioxidant Properties, and Iron Content of Underutilized and Commonly Consumed Fruits in Sri Lanka

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Hashini I. Abeysuriya ◽  
Vajira P. Bulugahapitiya ◽  
Jayatissa Loku Pulukkuttige
Keyword(s):  
Ascorbic Acid ◽  
Sri Lanka ◽  
Vitamin C ◽  
Low Cost ◽  
Antioxidant Properties ◽  
Dehydroascorbic Acid ◽  
Biochemical Properties ◽  
Total Phenolic ◽  
Total Vitamin ◽  
Frap Assay

Sri Lanka is rich in a wide diversity of fruits, but many are underutilized by the people in Sri Lanka despite their nutritional value. This is mainly due to little awareness of the palatability of many fruits and hence low popularity in the market. The present study aimed at providing comparative data on the main biochemical and nutritional parameters of thirty-seven (37) species of fruits grown in Sri Lanka, including 22 underutilized fruits and 15 commonly consumed fruits. The main parameters of the comparison were the contents of ascorbic acid (AA), total vitamin C (TVC), total phenolic content (TPC), total flavonoid content (TFC), total iron (Fe), and antioxidant capacities (ACs). The mean AA, TVC, TPC, TFC, and Fe contents in 100 g of fresh edible portions of fruits ranged from 2.0 to 185.0 mg, 8.1 to 529.6 mg, 12.9 to 2701.7 mg gallic acid equivalent, 0.2 to 117.5 mg quercetin equivalents, and 0.1 to 1.1 mg, respectively. The IC50 values in a DPPH assay varied between 0.8 to 1856.7 mg/mL and FRAP values in a FRAP assay ranged from 4.2 to 2070 μmol FeSO4/g in the studied fruits. Fruits were ranked based on the levels of the abovementioned biochemical properties. Using this ranking, 12 of the top 15 fruits were underutilized. Phyllanthus emblica (Indian gooseberry) is at the top of these underutilized fruits, and Psidium guajava (guava) is the best among commonly consumed fruits. These results indicate that underutilized fruits in Sri Lanka can be recommended as high quality and low-cost alternatives for securing nutritional requirements. Hence, underutilized fruits can be promoted as healthy additional fruits in Sri Lanka.

scholarly journals Antibacterial, Antibiofilm and Antioxidant Activities of some Medicinal Plants from Pharmacopoeia of Tassili N’ajjer

2020 ◽  
Vol 14 (3) ◽  
pp. 1835-1844
Author(s):  
Abdelkader Benhelima ◽  
Olivier Vidal ◽  
Zohra Kaid-Omar ◽  
Rabea Sahki ◽  
Jean-Marie Lacroix
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Confocal Scanning Laser Microscopy ◽  
Total Phenolic ◽  
Systematic Research ◽  
Biofilm Inhibition ◽  
Methanolic Extracts ◽  
Cymbopogon Schoenanthus

Three plants adopted by nomads at Tassili n’ajjer (south Algeria) in traditional medicine namely, Cymbopogon schoenanthus, Anabasis articulata and Salvia chudaei, were analysed for theirs antibacterial, antibiofilm and antioxidant properties. Total flavonoid and phenolic contents were measured with 2% AlCl3 and Folin-Ciocalteu’s reagent method, respectively. The antibacterial propertie was investigated by measurement of MIC of plants extract inhibing bacterial proliferation. The antibiofilm propertie was calculated by fluorescent quantization of the DAPI labeled bacterial biomass fixed on the surface and by COMSTAT analysis of confocal scanning laser microscopy (CSLM) images. DPPH radical scavenging and β-carotene/linoleate bleaching methods were used to determine the antioxidant activities of the plants. Total phenolic content was ranged from 21.98 to 2.51 (gallic acid equivalents mg/g methanolic extracts) and the total flavonoid content ranged from 19.27 to 1.65 (catechin equivalent mg/g methanolic extracts). Antibacterial activity against four Gram positive bacteria was shown with plants extracts. The biofilm inhibition concentration of extracts decreasing 50% of biofilm cell density (BIC50) for Salvia chudaei and Cymbopogon schoenanthus extracts ranges from 1 to 10 µg/mL. CSLM images analysis revealed that both surface covering by germ and three dimensional development of the biofilm were reduced with plants extracts. For antioxidant activities, the methanol extracts of the plants evaluated showed low antioxidant activity, with a IC50 between 1.94 and 6.16 mg/ml. Thus, Our systematic research showed that this three common plants of Sahara desert has diversified phytochemicals possessing satisfying extent of antimicrobial, antibiofilm and antioxidant activities.

scholarly journals Antibacterial and antioxidant activities of Homalomena josefii P.C. Boyce and S.Y. Wong rhizome extract

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2122-2131
Author(s):  
K.R. Rinai ◽  
I.S. Ismail ◽  
Son R. ◽  
C.Y. New ◽  
Y. Rukayadi
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Inhibition Zone ◽  
Total Phenolic ◽  
Crude Extracts ◽  
Snake Bites ◽  
Time Kill

The rhizome of Homalomena josefii P.C. Boyce and S.Y. Wong is commonly used in Borneo folk medicine. It is potent in treating stomach aches, headaches, and snake bites. The lack of information about their biological activities leads to an investigation to determine its antibacterial and antioxidant activities of H. josefii rhizome extract. This study aimed to ascertain antibacterial activity in opposition to foodborne pathogens namely Bacillus megaterium ATCC14581, Bacillus pumilus ATCC14884, Proteus mirabilis ATCC21100 and Klebsiella pneumoniae ATCC13773 and its antioxidant properties of H. josefii rhizome extracts. Extraction of dried powdered H. josefii rhizome was carried out through the maceration method using methanol as a solvent to produce crude extracts. The crude extracts were then tested for antibacterial and antioxidant activities. The antibacterial activity was conducted in terms of disc diffusion assay (DDA), minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time-kill assay. All were performed following the Clinical and Laboratory Standard Institute (CLSI) procedure to ensure liable results. Total phenolic compounds (TPC) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) scavenging assay were used to stimulate the antioxidant activity. The results show that DDA inhibition zone of the methanolic extracts ranged from 9.00±0.58 to 10.00±0.00 mm. The extract was able to inhibit the growth of all tested bacteria with MICs value ranging from 0.31 to 5.00 mg/mL. Meanwhile, the extract is able to kill all tested bacteria with MBC values that range from 0.63 to 5.00 mg/mL. Time-kill assay curve analysis results showed that the extract was able to completely kill the bacterial growth at 4 × MIC for 4 hours. The total phenolic compound (TPC) of methanolic extract was 435.138 mg GAE/g and IC50 of the extract was 11.809 μg/mL. In conclusion, the methanolic extract of H. josefii rhizome extract exhibited antibacterial and antioxidant activities, thus it can further be developed as a natural preservative.

scholarly journals Effects of Various Drying Methods on Selected Physical and Antioxidant Properties of Extracts from Moringa oliefera Leaf Waste

2020 ◽  
Vol 12 (20) ◽  
pp. 8586
Author(s):  
Ade Chandra Iwansyah ◽  
Tran Dinh Manh ◽  
Yusuf Andriana ◽  
Muhammad Aiman bin Hessan ◽  
Faridah Kormin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Physical Properties ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Drying Methods ◽  
Sun Drying ◽  
Phenolic Contents ◽  
Tray Drying

This study aims to evaluate the effects of different drying methods on the physical properties, total phenolic content, total flavonoid content, and antioxidant activity of extracts from Moringa oliefera L. (MO) leaf waste. The effects of two drying techniques, namely, sun drying (A1) and tray drying (A2), on the physical and antioxidant properties of the extracts obtained using three extracting solvents, i.e., water (b1), ethanol (b2), and ethyl acetate (b3), were investigated. These extracts were analyzed for their physicochemical and antioxidant properties. The antioxidant properties were determined with the Folin Ciocalteau, aluminum chloride, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Fourier transform infrared spectrophotometry (FTIR) was used to identify functional groups in the active compounds. It was found that the physical properties of the MO extract, including yield, pH, total solids, and color, showed significant differences for the two drying methods (p < 0.05). The b3 extract had the highest value for total phenolic contents, total flavonoid contents, and antioxidant activities, followed by b2 and b1, respectively, for both the sun drying and tray drying methods. There was a significant correlation between the total phenolic and flavonoid contents and antioxidant activity (IC50). This study reveals that waste material from MO leaves could be utilized as an antioxidant agent, which is expected to reduce environmental pollution from the MO processing industry.

scholarly journals Lonicera iberica M. Bieb.: Investigation Antioxidant Activity and Bioactive Chemicals

2021 ◽  
Vol 9 (6) ◽  
pp. 1124-1128
Author(s):  
Fatma Ergün
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Phenolic Substance ◽  
Amount Of Substance ◽  
Phenolic And Flavonoid

In this study, it was investigated the total amounts of phenolic and flavonoid substances and antioxidant activities in different solvent extracts of Lonicera iberica M. Bieb. wild fruit. Total phenolic contents of the extracts were determined as equivalent to gallic acid using Folin-Ciocalteu reagent, and total flavonoid contents as equivalent to quercetin by aluminium nitrate method. In addition, the antioxidant properties of the extracts were determined using free radical scavenging (DPPH) and reducing power (FRAP) methods. The amount of total phenolic substance of L. iberica fruits in hexane and methanol extracts was calculated as 30.96 ± 0.67 mg of GAE / g and 23.70 ± 1.56 mg of GAE / g, respectively. In addition, the amount of total flavonoid substance was calculated as 46.50 ± 8.54 mg of QE / g and 42.09 ± 2.58 mg of QE / g, respectively. It was determined that DPPH radical scavenging activity correlated with total phenolic and flavonoid amount of substance, and L. iberica had a strong antioxidant effect. It is predicted that our study will shed light on new researches, since it is the first study done with L. Iberica fruits in this field.

scholarly journals Phytochemical and Antioxidant Potential of Four Traditional Malaysian Medicinal Plants

2017 ◽  
Vol 5 (1) ◽  
pp. 9-14
Author(s):  
Norfarizan-Hanoon N.A ◽  
Nurraihana H ◽  
Hasmah A ◽  
Wan Rosli W.I.
Keyword(s):  
Medicinal Plants ◽  
Antioxidant Properties ◽  
Dry Weight ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Frap Assay ◽  
Total Antioxidant ◽  
Different Levels

Phytochemicals and antioxidants can be found at different levels in medicinal plants that are associated with the use in chronic diseases treatment. The objective of the present study was to investigate the phytochemical and antioxidant properties of Albizia myriophylla, Garcinia opaca var. dumosa, Oxalis barrelieri and Tacca cristata, traditional medicinal plants used in healthcare by aborigines or 'Orang Asli' from Kelantan, Malaysia. The total antioxidant capacity was estimated by ABTS (2,2’ azinobis-(3-ethybenzthiazoline-6-sulfonic acid)), DPPH (1,1-diphenyl-2-picrylhydrazyl radical) and FRAP assay. The results showed that the four selected plants had significant differences in their total phenolic content (ranging from 45.92 ± 2.01 to 613.37 ± 8.92 mg GAE/g dry weight), in their total flavonoid content (ranging from 6.03 ± 1.03 to 19.29 ± 2.90 mg CE/g dry weight), in their tannins (ranging from 28.23 ± 7.39 to 64.99 ± 7.27 mg TAE/g dry weight), in their alkaloids (ranging from 0.40 to 3.27%) and in their saponins (ranging from 4.31 to 13.15%). The TEAC values of the extracts ranged from 112.12 ± 3.16 to 237.20 ± 1.55 µmol Trolox/g dry weight, from 8.38 ± 2.09 to 219.36 ± 23.48 µmol Trolox/g dry weight and from 136.83 ± 5.24 to 220.93 ± 48.71 µmol Trolox/g dry weight, for ABTS, DPPH and FRAP respectively. TEAC values determined by ABTS, DPPH and FRAP were well correlated with phenolics, flavonoids and tannins contents. Garcinia opaca demonstrated the highest total phenolics and tannins while Oxalis barrelieri showed the highest total flavonoid and alkaloid compared to other plants studied. Garcinia opaca also had shown the highest antioxidant potential, followed by Oxalis barrelieri. This study had shown that Garcinia opaca var. dumosa and Oxalis barrelieri can be promoted as the source of phenolics antioxidant and radical scavengers.

scholarly journals Effect of Solvents on Bioactive Compounds and Antioxidant Activity of Padina Tetrastromatica and Gracilaria Tenuistipitata Seaweeds Collected From Bangladesh

2021 ◽  
Author(s):  
Mohammad Khairul Alam Sobuj ◽  
Md. Ariful Islam ◽  
Md. Shoebul Islam ◽  
Md. Mohidul Islam ◽  
Yahia Mahmud ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Bioactive Compound ◽  
Total Phenolic ◽  
Gracilaria Tenuistipitata ◽  
Padina Tetrastromatica

Abstract Seaweeds are now recognized as a treasure of bioactive compounds. However, the seaweed of Bangladesh is still unexplored. So, this study was designed to explore the secondary metabolites and antioxidant activities of solvent extracts of Padina tetrastromatica and Gracilaria tenuistipitata. Phytochemical screening and Fourier transform infrared (FTIR) confirm the diverse type of bioactive compounds. Antioxidant activity of extracts were evaluated by 1, 1-diphenyl-2- picrylhydrazyl (DPPH), 2, 2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), reducing power (RP), phosphomolybdenum and hydrogen peroxide assays. Here, methanolic extract of P. tetrastromatica showed the highest amount of total phenolic content (85.61 mg of GA/g), total flavonoid content (41.77 mg of quercetin/g), which showed as potent antioxidant properties confirmed by DPPH (77.07%), ABTS (77.65%), RP (53.24 mg AAE/g), phosphomolybdenum (31.58 mg AAE/g) and hydrogen peroxide (67.89%) assays followed by ethanol and water. This study concluded that bioactive compound and antioxidant activities are dose and solvent-dependent and seaweed type.

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