Dried distiller´s grains plus solubles supplementation improves low-quality tropical grass utilization on beef steers

ABSTRACT: This study was designed to evaluate the effect of corn dried distiller´s grains (DDGS) supplementation on feed intake, total tract digestibility, and ruminal fermentation of beef steers fed low-quality Guinea grass (Megathyrsus maximus, cv. Gatton panic). Twelve Braford crossbred steers were housed in individual pens (n = 4 steers/treatment), provided with three levels of DDGS supplement: 0%, 0.6%, or 1.2% BW. Steers were blocked by live weight and randomly assigned to treatments within the block. Corn DDGS supplementation increased total OM intake (21.55, 40.23, and 56.69 g/kg BW0.75) and tract OM digestibility (46.33, 49.03, and 72.39 % DM). Total tract digestible OM, CP, NDF and EE intake also increase in response to DDGS supplementation. Forage OM intake decreased when supplementation level reached 1.2 % BW. Also, ruminal pH decreased with DDGS supplementation level (6.88, 6.47, and 6.27). No differences were observed in total volatile fatty acids (VFA) concentration; however, the molar proportion of acetate decreased (77.98, 73.90, and 67.29 % Total VFA) as well as acetate: propionate ratio (4.38, 3.48, and 2.74). On the contrary, propionate proportions increased (18.32, 21.86, and 24.81 % Total VFA). Levels of ammonia and lactate were within suggested values for optimal fermentation and bacterial growth. Low-quality grass supplementation with corn DDGS increased total OM intake and digestibility. Also, DDGS inclusion favorably altered volatile fatty acids profile by reducing the acetate to propionate ratio regarding forage-only diets.

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Concentrate containing calcium salts of fatty acids rich in polyunsaturated fatty acids can change rumen fermentation in grazing goats

Semina Ciências Agrárias ◽

10.5433/1679-0359.2018v39n6p2621 ◽

2018 ◽

Vol 39 (6) ◽

pp. 2621

Author(s):

Ludmila Couto Gomes ◽

Claudete Regina Alcalde ◽

Julio Cesar Damasceno ◽

Luiz Paulo Rigolon ◽

Ana Paula Silva Possamai ◽

...

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Rumen Fluid ◽

Latin Square ◽

Rumen Fermentation ◽

Ruminal Fermentation ◽

Calcium Salts ◽

Linear Effect ◽

Concentrate Supplementation ◽

Ruminal Ph

Feeding goats with calcium salts of fatty acids (CSFA) can supply ruminants with lipids, with minimal effects on ruminal fermentation and fiber digestibility. However, there is a shortage of information on the effect of CSFA on characteristics of rumen fermentation in grassland goats. Thus, the present study aimed to assess the addition of CSFA to concentrate on the parameters of rumen fermentation of grazing goats. Five rumen cannulated goats were distributed in a Latin square 5x5 design (treatments: 0%, 1.5%, 3.0%, 4.5% and 6.0% CSFA. The pH, ammonia N and volatile fatty acids (VFA) content were analyzed in the ruminal fluid at 0, 2, 4, 6 and 8 hours after concentrate supplementation. The pH and ammonia N concentration showed a linear effect with the addition of CSFA. There was no effect observed for the VFA molar concentration after grazing goats were fed with the experimental diet. In conclusion, further research is needed to investigate the addition of CSFA to goat diets because there is evidence that CSFA increases ruminal pH and decreases excess ruminal ammonia without changing the VFA concentration in the rumen fluid.

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Effects of Tithonia diversifolia on in vitro methane production and ruminal fermentation characteristics

Animal Production Science ◽

10.1071/an15560 ◽

2016 ◽

Vol 56 (3) ◽

pp. 437 ◽

Cited By ~ 3

Author(s):

S. A. Terry ◽

R. S. Ribeiro ◽

D. S. Freitas ◽

G. D. Delarota ◽

L. G. R. Pereira ◽

...

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Fold Increase ◽

Gas Production ◽

Tithonia Diversifolia ◽

Ruminal Fermentation ◽

Total Volatile ◽

Treatment Groups ◽

Ch4 Production

The present study examined the effects of Tithonia diversifolia on in vitro methane (CH4) production and ruminal fermentation characteristics. The experiment was conducted as a completely randomised design (CRD) using a control (0% T. diversifolia) and three treatment groups with different concentrations (6.9%, 15.2%, 29.2%) of T. diversifolia, which replaced up to 15.2% and 14% dry matter (DM) of fresh sugarcane and concentrates, respectively. Ruminal fluid was obtained from two ruminally cannulated non-lactating Holstein × Zebu heifers maintained on a diet consisting of T. diversifolia, fresh sugarcane and 4 kg of concentrates. The inclusion of T. diversifolia had no effect (P ≥ 0.15) on cumulative gas production (mL, mL/g incubated DM, mL/g digested DM) or in vitro DM disappearance (%). Carbon dioxide (%, mL, mL/g incubated DM) linearly decreased (P ≤ 0.001) and CH4 (%, mL, mL/g incubated DM) quadratically increased (P ≤ 0.01) with increasing concentrations of T. diversifolia replacing fresh sugarcane and concentrates. The total volatile fatty acids (mM) and acetate (A) proportion of total volatile fatty acids (mmol/100 mmol) linearly increased (P < 0.01) with the increasing inclusion of T. diversifolia. Butyrate (mmol/100 mmol) increased quadratically (P ≤ 0.02), while propionate (P; mmol/100 mmol) decreased quadratically (P < 0.02). The A : P ratio increased linearly (P < 0.0001) with increasing amounts of T. diversifolia in the diet. These results indicated that increasing the amount of Tithonia diversifolia in the substrate DM increased the A : P ratio, which resulted in a six-fold increase of CH4 production when fresh sugarcane and concentrates were replaced at up to 15.2% and 14% (DM basis), respectively.

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The contribution of propionate to glucose synthesis in sheep given diets of different grain content

British Journal Of Nutrition ◽

10.1079/bjn19680009 ◽

1968 ◽

Vol 22 (1) ◽

pp. 69-75 ◽

Cited By ~ 50

Author(s):

G. J. Judson ◽

Elizabeth Anderson ◽

J. R. Luick ◽

R. A. Leng

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Glucose Absorption ◽

Ruminal Fermentation ◽

Total Volatile ◽

Production Rates ◽

Entry Rate ◽

Ruminal Fluid ◽

Glucose Synthesis ◽

The Mean

1. Glucose entry rates into the blood and propionate production rates in the rumen have been measured in sheep given rations containing varying proportions of starch and roughage (lucerne).2. Glucose entry rates and propionate production rates were similar for all rations studied.3. The proportion of the glucose entry rate arising from propionate produced in the rumen was highest on the ration containing the greatest quantity of lucerne and decreased as the proportion of starch in the ration increased. Rate of conversion of propionate into glucose was estimated and was found to decrease as the amount of starch in the ration increased.4. Concentrations of total volatile fatty acids (VFA) in the ruminal fluid were lowest in the ration with the greatest proportion of starch, implying lower VFA production rates with the starch rations although intakes of digestible energy were approximately the same. The mean concentrations and rates of production of propionate in ruminal fluid were similar for all rations.5. The low VFA concentrations and the reduced conversion of propionate into glucose on the high rations, despite similar propionate production rates and glucose entry rates, may have been due to starch escaping ruminal fermentation. It is suggested that this glucose absorption may have reduced gluconeogenesis from propionate.

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Effect of type and feeding frequency of high-fibre feeds on intake, total tract digestibility and ruminal fermentation in beef steers fed a high-concentrate diet

Animal Production Science ◽

10.1071/an18014 ◽

2019 ◽

Vol 59 (12) ◽

pp. 2245

Author(s):

A. López ◽

H. O. Coria ◽

M. A. Nazareno ◽

E. M. García ◽

R. A. Distel ◽

...

Keyword(s):

Volatile Fatty Acids ◽

Ammonia Concentration ◽

Similar Response ◽

Beef Steers ◽

Ruminal Fermentation ◽

Total Volatile ◽

Alfalfa Hay ◽

Volatile Fatty Acid Concentration ◽

Economic Constraints ◽

By Products

The inclusion of fibre in a total mixed ration (TMR) often has operational and economic constraints. The objective of the present study was to compare intake and ruminal fermentation of steers fed either a TMR or a diet with the fibre fraction fed separately from the concentrate fraction. Six ruminally fistulated steers were used in a six-treatments, four-period cross-over experiment. Treatment structure was a 3 × 2 factorial. The first factor was fibre delivery either as a part of a TMR or offered separately from the concentrate fraction once every 3 or 6 days. The second factor was represented by two fibre sources, namely, alfalfa hay or cotton by-products. Fibre delivery and source did not significantly affect total, concentrate, fibre and digestible dry-matter intake, compared with TMR. A similar response was observed for ruminal pH, ammonia concentration and total volatile fatty acid concentration. Intake of crude protein and fibre, as well as the concentration of total volatile fatty acids, were higher for alfalfa hay than for cotton by-products. In conclusion, feeding the fibre portion separately from the concentrate fraction once every 3 or 6 days did not negatively affect intake and rumen function compared with when a TMR was fed, regardless of fibre source.

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Effect of dietarysupplementation with treated amaranth seeds on fermentation parameters in an artificial rumen

Czech Journal of Animal Science ◽

10.17221/6710-cjas ◽

2013 ◽

Vol 58 (No. 4) ◽

pp. 159-166 ◽

Cited By ~ 1

Author(s):

P. Kubelková ◽

D. Jalč ◽

P. Homolka ◽

B. Čermák

Keyword(s):

Fatty Acids ◽

Crude Protein ◽

Dry Matter ◽

Volatile Fatty Acids ◽

Control Diet ◽

Ruminal Fermentation ◽

Total Volatile ◽

Microbial Nitrogen ◽

Acid Detergent Fibre ◽

Fermentation Parameters

The rumen simulating technique (RUSITEC) was used to evaluate the effect of dietary inclusion of amaranth seeds on the rumen fermentation and the concentration of fatty acids (FA) in fermentation fluid. Four fermentation vessels of the RUSITEC were used. The control diet (C) consisted of 70% meadow hay and 30% barley meal, whereas 10% of barley meal was replaced by milled (Amill), mechanically ground (Agr), and ground after heating in a microwave amaranth seeds (Aheat+gr) in other experimental vessels, respectively. All diets were isonitrogenous (11% of crude protein). With degradability of dry matter (DM) and acid-detergent fibre (ADF) not affected (P > 0.05), degradability of neutral detergent fibre (NDF) was significantly decreased by the inclusion of Amill (P < 0.001) and Aheat+gr (P < 0.05). Heating, compared to milling, decreased degradation of crude protein (CP) and addition of amaranth seeds generally increased production of microbial nitrogen. Production of fermentation gasses, methane, and total volatile fatty acids (VFA) was not affected by changes in composition of diets. Addition of amaranth seeds in a milled form (Amill) caused a significant decrease in concentrations of acetate and propionate and growth in concentrations of butyrate, iso-valerate, and caproate. No effect (P > 0.05), compared to C, was found on the percentage of saturated or unsaturated FA, but changes in concentrations of some FA were observed. An absence of any detrimental effects on ruminal fermentation patterns indicated that grain amaranth seeds can partially substitute for the barley in ruminant nutrition.

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Effects of feeding wheat middlings on production, digestibility, ruminal fermentation and carcass characteristics in beef cattle

Canadian Journal of Animal Science ◽

10.4141/a02-086 ◽

2003 ◽

Vol 83 (3) ◽

pp. 551-557 ◽

Cited By ~ 3

Author(s):

D. R. ZoBell ◽

L. A. Goonewardene ◽

K. C. Olson ◽

C. A. Stonecipher ◽

R. D. Wiedmeier

Keyword(s):

Fatty Acids ◽

Beef Cattle ◽

Dry Matter ◽

Volatile Fatty Acids ◽

Average Daily Gain ◽

Carcass Characteristics ◽

Ruminal Fermentation ◽

Total Volatile ◽

Wheat Middlings ◽

Daily Gain

Studies were conducted to compare the feed value of wheat middlings (WM) to cereal grains in rations with varying concentrate to roughage levels. In two separate studies, weaned heifers and steers were fed corn silage/alfalf a hay-based growing diets where the concentrate source was either a control diet consisting of rolled barley (heifers) or rolled corn (steers) versus WM, over an 84-d period. In a 107-d study, finishing steers were fed treatments that consisted of rolled corn (C) plus either 35% wheat middlings (WM35) or 50% wheat middlings (WM50). A single cross-over designed digestibility study was also conducted utilizing four cannulated yearling heifers fed either a corn or WM50 diet from the finishing steer study. Results from the heifer and growing and finishing steer studies showed that average daily gain (ADG), dry matter intake (DMI) and feed efficience (FE) were not affected (P > 0.05) by feeding treatment. Carcass characteristics, including hot carcass weight, ribeye area, backfat, yield, quality grade and cutability were also unaffected (P > 0.05) by feed treatment. Results from the digestibility studies indicated total volatile fatty acids (VFA) were increased (P = 0.023) in the WM diet, with acetate lower (P = 0.0003) than the C group, and pH levels of 5.81 and 5.55 for the C and WM treatments respectively (P = 0.011). Dry matter and ADF digestibilities were not affected (P > 0.05) by feed treatment. It is concluded from these studies that WM can be fed to growing beef heifers and steers as an alternative to more traditional concentrate sources such as corn or barley. Wheat middlings could replace up to 50% of the concentrate in a finishing ration. However, certain ruminal fermentation characteristics such as pH, acetate: propionate ratio and total volatile fatty acids may be altered, which could affect performance over an extended feeding period. Key words: Wheat middlings, average daily gain, feed efficiency, digestibility, ruminal degradation, beef cattle

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Effects of Increasing Doses of Condensed Tannins Extract from Cistus ladanifer L. on In Vitro Ruminal Fermentation and Biohydrogenation

Animals ◽

10.3390/ani11030761 ◽

2021 ◽

Vol 11 (3) ◽

pp. 761

Author(s):

Olinda Guerreiro ◽

Susana P. Alves ◽

Mónica Costa ◽

Maria F. Duarte ◽

Eliana Jerónimo ◽

...

Keyword(s):

Fatty Acids ◽

Condensed Tannins ◽

Volatile Fatty Acids ◽

Secondary Compounds ◽

Cistus Ladanifer ◽

Ruminal Fermentation ◽

Experimental Conditions ◽

Ct Dose ◽

Linear Decrease

Cistus ladanifer (rockrose) is a perennial shrub quite abundant in the Mediterranean region, and it is a rich source in secondary compounds such as condensed tannins (CTs). Condensed tannins from C. ladanifer were able to change the ruminal biohydrogenation (BH), increasing the t11–18:1 and c9,t11–18:2 production. However, the adequate conditions of the C. ladanifer CTs used to optimize the production of t11–18:1 and c9,t11–18:2 is not yet known. Thus, we tested the effect of increasing the doses of C. ladanifer CT extract (0, 25, 50, 75 and 100 g/kg dry matter (DM)) on in vitro rumen BH. Five in vitro batch incubations replicates were conducted using an oil supplemented high-concentrate substrate, incubated for 24 h with 6 mL of buffered ruminal fluid. Volatile fatty acids (VFAs) and long chain fatty acids (FA) were analyzed at 0 h and 24 h, and BH of c9–18:1, c9, c12–18:2 and c9, c12, c15–18:3, and BH products yield were computed. Increasing doses of C. ladanifer CTs led to a moderate linear decrease (p < 0.001) of the VFA production (a reduction of 27% with the highest dose compared to control). The disappearance of c9–18:1 and c9,c12–18:2 as well as the production of t11–18:1 and c9, t11:18:2 was not affected by increasing doses of C. ladanifer CTs, and only the disappearance of c9, c12, c15–18:3 suffered a mild linear decrease (a reduction of 24% with the highest dose compared to control). Nevertheless, increasing the C. ladanifer CT dose led to a strong depression of microbial odd and branched fatty acids and of dimethyl acetals production (less than 65% with the highest dose compared to control), which indicates that microbial growth was more inhibited than fermentative and biohydrogenation activities, in a possible adaptative response of microbial population to stress induced to CTs and polyunsaturated fatty acids. The ability of C. ladanifer to modulate the ruminal BH was not verified in the current in vitro experimental conditions, emphasizing the inconsistent BH response to CTs and highlighting the need to continue seeking the optimal conditions for using CTs to improve the fatty acid profile of ruminant fat.

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Further studies on the effects of dietary cottonseed oil on milk-fat secretion in the cow

Journal of Dairy Research ◽

10.1017/s0022029900019075 ◽

1968 ◽

Vol 35 (3) ◽

pp. 343-352 ◽

Cited By ~ 22

Author(s):

W. Steele ◽

J. H. Moore

Keyword(s):

Fatty Acids ◽

Milk Fat ◽

Volatile Fatty Acids ◽

Octadecenoic Acid ◽

Cottonseed Oil ◽

Rumen Fermentation ◽

Total Volatile ◽

Feeding Experiments ◽

Concentrate Mixture ◽

Low Levels

SummaryThe effects of the isocaloric replacement of part of the dietary concentrate mixture by cottonseed oil on the yield and composition of the milk fat and on the pattern of rumen fermentation was investigated in 2 feeding experiments with a total of 8 cows in mid-lactation. The concentrate mixtures were given with high- or low-roughage diets that supplied 9·1 or 1·8 kg of hay/day.In expt 1 the yield of milk fat was not altered by the addition of 10% cottonseed oil to the concentrate mixtures given either with the high- or with the low-levels of dietary roughage. On the low-roughage treatment, dietary cottonseed oil increased milk yield but reduced the fat content of the milk. The change from the high- to the low-roughage diets containing no cottonseed oil resulted in reductions in the yield and percentage of fat in the milk. In expt 2 the yields of milk and milk fat were similar irrespective of whether the concentrate mixture contained 5 or 10% cottonseed oil.In expt 1 the inclusion of 10% cottonseed oil in the concentrate mixture reduced the yields and percentages of the medium-chain fatty acids (12:0, 14:0 and 16:0) and increased the yields and percentages of the C18 fatty acids in the milk fat. In expt 2, when the concentrate mixture contained 5% cottonseed oil, the yields and percentages of all the fatty acids in the milk fat were similar to the values obtained when the concentrates contained 10% cottonseed oil. For any given concentrate mixture, the change from the high- to the low-roughage treatments in both expts 1 and 2 resulted in increases in the percentage of oleic acid in the milk fat. The highest concentration of trans-octadecenoic acid was observed in the milk fat of the cows when they were given the high-roughage diet with the concentrate mixture containing 10% cottonseed oil.In expt 2 the level of cottonseed oil in the concentrate mixture did not influence the pattern of rumen fermentation as measured by the concentrations of the various volatile fatty acids in the rumen liquor. However, the change from the high- to the low-roughage diets reduced the proportion of acetic and increased the proportions of propionic and n-valeric acids in the total volatile fatty acids in the rumen liquor.

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Metabolism of propane-1:2-diol infused into the rumen of sheep

British Journal Of Nutrition ◽

10.1079/bjn19720125 ◽

1972 ◽

Vol 27 (3) ◽

pp. 553-560 ◽

Cited By ~ 21

Author(s):

J. L. Clapperton ◽

J. W. Czerkawski

Keyword(s):

Carbon Dioxide ◽

Fatty Acids ◽

Acetic Acid ◽

Lactic Acid ◽

Polyethylene Glycol ◽

Energy Metabolism ◽

Heat Production ◽

Volatile Fatty Acids ◽

Carbon Dioxide Production ◽

Total Volatile

1. Propane-1:2-diol (loog/d) was infused through a cannula into the rumen of sheep receiving a ration of hay and dried grass. The concentration of volatile fatty acids, propanediol, lactic acid and of added polyethylene glycol, and the pH of the rumen contents were measured. The energy metabolism of the sheep was also determined.2. Most of the propanediol disappeared from the rumen within 4 h of its infusion. The infusion of propanediol resulted in a 10% decrease in the concentration of total volatile acids; the concentration of acetic acid decreased by about 30%, that of propionic acid increased by up to 60% and there was no change in the concentration of butyric acid.3. The methane production of the sheep decreased by about 9% after the infusion of propanediol and there were increases in the oxgyen consumption, carbon dioxide production and heat production of the animals; each of these increases was equivalent to about 40% of the theoretical value for the complete metabolism of 100 g propanediol.4. It is concluded that, when propanediol is introduced into the rumen, a proportion is metabolized in the rumen and a large proportion is absorbed directly. Our thanks are due to Dr J. H. Moore for helpful discussions, to Mr D. R. Paterson, Mr J. R. McDill and Mr C. E. Park for looking after the animals and to Miss K. M. Graham, Miss A. T. McKay and Mrs C. E. Ramage for performing the analyses.

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