scholarly journals Prevalence of cutaneous leishmaniasis in the largest populated city Karachi, Pakistan

2023 ◽  
Vol 83 ◽  
Author(s):  
Ihsanullah ◽  
M. J. Yousuf ◽  
N. Ahmad ◽  
Z. Masood ◽  
S. Majeed ◽  
...  

Abstract. The study was undertaken from September 2018 to April 2019 to determine the prevalence of cutaneous leishmaniasis in human beings living in six districts of Karachi. Suspected persons were screened for the disease and positive cases were identified on the basis of skin lesions and blood samples. Samples were observed by mounting their smear. A total of 207 subjects of different ages and sex groups were investigated, however, only 192 (92%) of the suspected cases were found to have the disease 64% of cases were male which were significantly high (p<0.05), than female 36%. The lesion was more frequently detected among the youth ages of 21-30 years (31%) as compared to other groups. In both sexes, legs were found to be more infected (25% male + 20% female) followed by arms (20% male + 0% female) and face (15% male +11% female). The mixed body parts had shown the lowest infections such as (4% in males + 5%) in females. In conclusion, the highest and lowest leishmaniasis infections were observed in District West (23% male + 9% female) followed by District East (15% male + 7% female), District Malir (11% male+ 4% female), District Central (7% male + 5% female), District Korangi (4% male + 7% female) and District South (4% male + 4% female) respectively.

Author(s):  
Abdullah D. ALANAZI ◽  
Robert PUSCHENDORF ◽  
Mohamed S. ALYOUSIF ◽  
Mohamed S. Al-KHALIFA ◽  
Samir A. ALHARBI ◽  
...  

Background: Dogs can act as reservoirs of canine leishmaniasis, caused by Leishmania species. The aims of this study were to determine the prevalence of canine leishmaniasis using a PCR technique among stray dogs living in three provinces of Saudi Arabia, Riyadh, Al-Ahsa Oasis and Al-Qaseem, where the disease is endemic; and to identify and document different Leishmania to species levels Methods: This cross-sectional investigation was conducted, from Mar 2016 to Apr 2018, in three parts of Saudi Arabia: Central province (Riyadh), Eastern province (Al-Ahsa Oasis) and Al-Qaseem province. Blood samples were collected from 526 dogs; 40 presented cutaneous nodules so were suspected clinically of cutaneous leishmaniasis. Biopsy tissue collections and parasite cultures were performed. A generic kDNA was performed using different primers for Leishmania differentiation. Results: All blood samples were negative for Leishmania infantum infection by molecular analysis, though forty dogs had thick cutaneous lesions in different parts of their body. Four dogs’ skin lesions were associated with dermatitis, splenomegaly and lymphadenomegaly. Parasite culture was used to diagnose cutaneous leishmaniasis, identifying 31/40 (77.5%) positive samples. Overall, of 526 samples, the prevalence of L. major and L. tropica was found to be 4% and 1.9%, respectively. Gender and age had a significant effect on Leishmania prevalence: (P=0.0212 and 0.0357), respectively. Conclusion: This was the first molecular study of dog leishmaniasis from Saudi Arabia of dogs confirmed to have cutaneous leishmaniasis. Further epidemiological and molecular investigations of domestic and wild canine infections with L. major, L. tropica and L. infantum in endemic and nonendemic areas of Saudi Arabia are required, for leishmaniasis control.


2021 ◽  
Vol 49 (1) ◽  
Author(s):  
Hiroyuki Kitano ◽  
Chizu Sanjoba ◽  
Yasuyuki Goto ◽  
Kazumasa Iwamoto ◽  
Hiroki Kitagawa ◽  
...  

Abstract Background Leishmaniasis is not endemic in Japan, and imported cases are rare. However, there are increasing concerns regarding imported cases of cutaneous leishmaniasis from endemic countries to Japan. This report describes a case of imported cutaneous leishmaniasis that was diagnosed and treated in Japan. Case presentation A 53-year-old Pakistani man presented with skin lesions on both malleoli of his right ankle and the dorsum of the left foot. The skin lesions manifested as erythematous nodules surrounding an ulcer in the center of the lesion. The lesions of the malleoli of his right ankle each measured 3 × 3 cm, and the lesion on the top of his left foot measured 5 × 4 cm. He had been living and working in Japan but had a history of a visit to Pakistan for about 2 months in 2018. The skin lesions were biopsied. Giemsa and hematoxylin and eosin staining of biopsy samples showed amastigotes of Leishmania in macrophages, and the presence of Leishmania was confirmed by skin tissue culture. Polymerase chain reaction using biopsy specimens identified Leishmania parasites, and DNA sequence analysis revealed that the species was Leishmania tropica. The patient was treated with intravenous liposomal amphotericin B for 6 days. The erythema disappeared, and the erythematous nodules resolved within 3 weeks. Conclusion This is the first report of imported cutaneous leishmaniasis caused by L. tropica from Pakistan, and it is interesting that all three testing modalities showed positive results in this case.


2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S428-S428
Author(s):  
Jennifer Jubulis ◽  
Amanda Goddard ◽  
Elizabeth Seiverling ◽  
Marc Kimball ◽  
Carol A McCarthy

Abstract Background Leishmaniasis has many clinical manifestations and treatment regimens, dependent on species and host. Old world leishmaniasis is found primarily in Africa and Asia, and is associated with visceral disease, while new world disease, seen primarily in Latin America, is more commonly mucocutaneous. We present a case series of pediatric African patients with New World cutaneous leishmaniasis (NWCL). Methods Data extraction was performed via chart review, analyzing travel history, clinical presentation, diagnosis, and management in children with cutaneous leishmaniasis presenting to the pediatric infectious diseases clinic in Portland, ME. Biopsy specimens were sent to the federal CDC for identification by PCR and culture. Results Five cases of NWCL were diagnosed in pediatric patients in Maine from November 2018 through February 2020. Median age of patients was 10 years (range 1.5-15 years). Four cases (80%) occurred in children from Angola or Democratic Republic of Congo, arriving in Maine via Central/South America, with one case in a child from Rwanda who arrived in Maine via Texas. Three patients had multiple skin lesions and two had isolated facial lesions. Leishmaniasis was not initially suspected resulting in median time to diagnosis of 5 months (range 1-7 months). Four patients were initially treated with antibacterials for cellulitis and one was treated with griseofulvin. After no improvement, patients underwent biopsy with 2 patients diagnosed with L panamensis, 1 with L braziliensis, 1 with mixed infection (L panamensis and L mexicana), and 1 with Leishmania species only. One patient was managed with surgical excision, 3 with ketoconazole, and 1 was observed off therapy. Four patients were referred to otolaryngology. All continue to be followed in infectious disease clinic. Conclusion We present five cases of new world cutaneous leishmaniasis in African pediatric patients arriving to Maine through Latin America or Texas. Patients were diagnosed with cellulitis, tinea corporis or atopic dermatitis initially, underscoring importance of high index of suspicion in migrant patients. Detailed travel history and epidemiologic knowledge is essential to diagnosis, as patients may present with illness not congruent with country of origin. Optimal therapy remains unclear. Disclosures All Authors: No reported disclosures


Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 404
Author(s):  
Kevin Van Tichelen ◽  
Sara Prims ◽  
Miriam Ayuso ◽  
Céline Van Kerschaver ◽  
Mario Vandaele ◽  
...  

The increase in litter sizes in recent years has resulted in more low birth weight (LBW) piglets, accompanied by a higher mortality. A potential intervention to overcome this is drenching bioactive substances. However, if the act of drenching provokes additional stress in LBW piglets, it might counteract the supplement’s effect and be detrimental for the piglet’s survival. To study the effect of the drenching act, piglets from 67 sows were weighed within 4 h after birth. The mean litter birth weight (MLBW) and standard deviation (SD) were calculated. LBW piglets (n = 76) were defined as weighing between (MLBW-1*SD) and (MLBW-2.5*SD). They were randomly allocated to two treatments: “sham” (conducting the act of drenching by inserting an empty 2.5 mL syringe in the mouth during 20 s, once a day, d1 till d7; n = 37) or “no treatment” (no handling; n = 39). On day 1, 3, 9, 24 and 38, piglets were weighed and scored for skin lesions. Blood samples were collected on day 9 and 38 and analyzed to determine glucose, non-esterified fatty acids (NEFA), urea, immunoglobulin G (IgG), insulin-like growth factor 1 (IGF-1) and a standard blood panel test. There was no difference between sham drenched and untreated piglets regarding any of the parameters. In conclusion, this study showed that drenching does not impose a significant risk to LBW piglets and can be applied safely during the first 7 days after birth.


2012 ◽  
Vol 54 (4) ◽  
pp. 215-218 ◽  
Author(s):  
Wilsandrei Cella ◽  
Simone Cristina Castanho Sabaini de Melo ◽  
Cátia Millene Dell Agnolo ◽  
Sandra Marisa Pelloso ◽  
Thaís Gomes Verzignassi Silveira ◽  
...  

We reviewed the records of 151 patients diagnosed with American cutaneous leishmaniasis (ACL) from 1993 to 2009 in the municipality of Japura, Paraná, Brazil. Gender, age, occupation, place of residence, location of lesions, type and number of lesions were analyzed. The prevalence rate of ACL was 11.5/10,000 hab, of which 84.7% were male, 58.3% lived in rural area and 49.0% were farmers. The most frequent age group was between 30 to 39 years (26.6%). Skin lesions occurred in 92.7% of the patients with predominance in the lower limbs (23.9%) and 49.1% of the records did not include the number of lesions location due to incomplete filling. A single ulceration was present in 44.4%. Japurá is an endemic area for ACL, requiring public actions and preventive education.


2004 ◽  
pp. 147-176
Author(s):  
Stanimir Rakic

In this paper I examine compound names of plants, animals, human beings and other things in which at least one nominal component designates a part of the body or clothes, or some basic elements of houshold in Serbian and English. The object of my analysis are complex derivatives of the type (adjective noun) + suffix in Serbian and componds of the type noun's + noun, noun + noun and adjective + noun in English. I try to show that there is a difference in metaphorical designation of human beings and other living creatures and things by such compound nouns. My thesis is that the metathorical designation of human beings by such compounds is based on the symbolic meaning of some words and expressions while the designation of other things and beings relies on noticed similarity. In Serbian language such designation is provided by comples derivatives praznoglavac 'empty-headed person', tupoglavac 'dullard' debolokoiac 'callos person', golobradac 'young, inexperienced person' zutokljunac 'tledling' (fig), in English chicken liver, beetle brain birdbrain, bonehead, butterfingers, bigwig, blackleg, blue blood bluestocking, eat's paw, deadhead,fat-guts,fathead, goldbrick (kol) hardhat, hardhead, greenhorn, redcoat (ist), redneck (sl), thickhead, etc. Polisemous compounds like eat's paw lend support for this thesis because their designation of human beings is based on symbolic meaning of some words or expressions. I hypothesize that the direction and extend of the possible metaphorization of names may be accounted for by the following hierarchy (11) people - animals - plants - meterial things. Such hierarchy is well supported by the observations of Lakoff (1987) and Taylor (1995) about the role of human body in early experience and perception ofthe reality. Different restrictions which may be imposed in the hierarchy (11) should be the matter of further study, some of which have been noted on this paper. The compounds of this type denoting people have metaphorically meaning conected with some pejorative uses. These compounds refer to some psychological or characteral features, and show that for the classification of people such features are much more important than physical properties. While the animals and plants are classified according to some charecteristics of their body parts, people are usually classified according to psychollogical characteristics or their social functions. I have also noted a difference in structure between compounds designation animals and those designating plants and other things. The designation of animals relies more on metonymy, and that of plants and other things on metaphor based on comparision of noticed similarities. In the compounds designating animals, the nominal component relatively seldom refers to the parts of plants or other things. I guess that the cause may be the fact that the anatomy of plants is very different from the anatomy of animals. As a consequence the structure adjective + noun is much more characteristic of the compounds designating animals in English than the structure noun's + noun, and the same holds, although in a lesser degree for the compounds designating humans. It is also noticeable that in English compounds whose second component a part of body or clothes the first component rarely designates animals. On the other hand the compounds (9), in which the nominal head refers to some superordinate species, the first component often designates animal species, but usually of a very different kind. These data seem to lend support for Goldvarg & Gluksberg's thesis (1998) that metaforical interpretation is favoured if the nominal constituents denote quite different entities.


2021 ◽  
Vol 5 (1) ◽  
pp. 247-262
Author(s):  
Nguyen Anh Quoc ◽  
Nguyen Minh Tri ◽  
Nguyen Anh Thuong ◽  
Dinh The Hoang ◽  
Nguyen Van Bung

Man and nature is a unity between body and individual in behavior. Humans are liberty, creative, happy subjects in behavior and labor. By behavior and labor, humans produce tools, spare parts, machines, and robots to replace internal organs, lengthen the senses, and lengthen defective body parts. Evolution is no longer a mutation in the body but the assembly of accessories into organs, senses, and body parts when needed. People use devices that are manufactured to be used for what people want depending on specific conditions and circumstances. Labor and behavior make objectification of people, but alienated behavior and alienated labor make humanize the object. The time to enjoy liberty, creativity, and happiness is human, and the time to perform alienated behavior and alienated labor is the time to live for the non-human. People are corrupted into slavery to standards, money. It is the process of self-torture, torturing oneself; and the nobility of standards, the wealth of money is the unhappy product of life. Humans are liberty, creative and happy subjects; alienated human beings are all helpless, unhappy, deceit. Money, standards are products of helplessness, unhappiness, lies. Standards, money remove people from life.


2019 ◽  
Vol 1 (17) ◽  
Author(s):  
U. Ayman ◽  
S. K. Das

Background: Papillomatosis is a viral disease manifested with benign cutaneous growths (skin epithelium hyperplasia) in different body parts which is encountered in cattle, goat, dog, rabbit, horse, rodent and also in human. The purpose of the present study is to detect the histo-morphological changes of cutaneous papillomatosis in indigenous cattle. Methods: Ten (10) random samples from skin lesions of warts were collected from adult indigenous cattle (10 cattle) that were diagnosed as cutaneous papillomatosis by clinical examination at Veterinary Teaching Hospital, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. For gross observations, location of appearance of warts, color and size was taken into consideration. Immediately after collection of sample, they were preserved in Bouin’s fluid followed by dehydration, clearing and infiltration and finally embedded by paraffin wax. Then samples were sectioned using rotatory microtome and stained with Harris’s Haematoxylin and Eosin Y to facilitate microscopic study. Results: The results of present study revealed that cutaneous papillomas were mostly located on the head and neck, around eyes, under the chin and perineal region in indigenous cattle. Grossly, warts in cattle are bumpy or cauliflower-like masses of various sizes and numbers that projected from the skin having grey, rough, scaly, and dry surface. When incised, its cut surface has an external layer- keratinized epithelium and an internal core- moist white connective tissue. Histopathological changes indicate hyperplastic features of the affected skin layers of epidermis. Further, the microscopic analysis also revealed orthokeratotic hyperkeratosis, acanthosis and down word hyperplasia, fibrovascular area, keratohyalin granules and koilocytes at different layers of the affected skin. Conclusion: The results of this present study might be useful for the classification and treatment of this common malady based on its histological changes.


Author(s):  
Hirotaka Osawa ◽  
◽  
Jun Mukai ◽  
Michita Imai ◽  

We propose an anthropomorphization framework that determines an object’s body image. This framework directly intervenes and anthropomorphizes objects in ubiquitous-computing environments through robotic body parts shaped like those of human beings, which provide information through spoken directions and body language. Our purpose is to demonstrate that an object acquires subjective representations through anthropomorphization. Using this framework, people can more fully understand instructions given by an object. We designed an anthropomorphization framework that changes the body image by attaching body parts. We also conducted experiments to evaluate this framework. Results indicate that the site at which an anthropomorphization device is attached influences human perception of the object’s virtual body image, and participants in experiments understood several instructions given by the object more clearly. Results also indicate that participants better intuited their devices’ instructions and movement in ubiquitous-computing environments.


2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Carmen M. Sandoval Pacheco ◽  
Gabriela V. Araujo Flores ◽  
Kadir Gonzalez ◽  
Claudia M. de Castro Gomes ◽  
Luiz F. D. Passero ◽  
...  

Macrophages play important roles in the innate and acquired immune responses against Leishmania parasites. Depending on the subset and activation status, macrophages may eliminate intracellular parasites; however, these host cells also can offer a safe environment for Leishmania replication. In this sense, the fate of the parasite may be influenced by the phenotype of the infected macrophage, linked to the subtype of classically activated (M1) or alternatively activated (M2) macrophages. In the present study, M1 and M2 macrophage subsets were analyzed by double-staining immunohistochemistry in skin biopsies from patients with American cutaneous leishmaniasis (ACL) caused by L. (L.) amazonensis, L. (V.) braziliensis, L. (V.) panamensis ,and L. (L.) infantum chagasi. High number of M1 macrophages was detected in nonulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi ( M 1 = 112 ± 12 , M 2 = 43 ± 12 cells/mm2). On the other side, high density of M2 macrophages was observed in the skin lesions of patients with anergic diffuse cutaneous leishmaniasis (ADCL) ( M 1 = 195 ± 25 , M 2 = 616 ± 114 ), followed by cases of localized cutaneous leishmaniasis (LCL) caused by L. (L.) amazonensis ( M 1 = 97 ± 24 , M 2 = 219 ± 29 ), L. (V.) panamensis ( M 1 = 71 ± 14 , M 2 = 164 ± 14 ), and L. (V.) braziliensis ( M 1 = 50 ± 13 , M 2 = 53 ± 10 ); however, low density of M2 macrophages was observed in NUCL. The data presented herein show the polarization of macrophages in skin lesions caused by different Leishmania species that may be related with the outcome of the disease.


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