Efektivitas Ekstrak Daun Babadotan Sebagai Green Antiseptic untuk Pencelup Puting Sapi Perah

ABSTRAK. Penelitian dilakukan untuk mengetahui efektifitas ekstrak daun babadotan sebagai bahan aktif cairan pencelup puting (teat dipping) untuk menghambat masuknya bakteri melalui puting, serta membandingkan efektifitasnya dengan povidone iodine. Dua belas ekor sapi perah laktasi penderita mastitis subklinis digunakan dalam rancangan acak lengkap (RAL). Perlakuan berupa teat dipping menggunakan ekstrak daun babadotan 5%, 10%, 15% dan povidone iodine 10% selama 14 hari. Tingkat peradangan kelenjar ambing diketahui dari penjumlahan skor California mastitis test (CMT) dari keempat puting sapi. Hasil penelitian menunjukan penurunan rata-rata total koloni bakteri dalam susu yang sangat signifikan (P0.01) dari 6,1-7,3 log cfu/ml sebelum pencelupan menjadi 4,4-6,0 log cfu/ml sesudah pencelupan. Tidak ada perbedaan efektifitas sebagai antibakteri antara ekstrak daun babadotan dan povidone iodine dalam menurunkan total koloni bakteri dalam susu. Terjadi penurunan tingkat peradangan kelenjar ambing (P0.01) dari rata-rata 6,9 sebelum teat dipping menjadi 4,2 sesudah teat dipping. Persentase penurunan tingkat peradangan ambing ekstrak daun babadotan lebih tinggi (P0.05) dari povidone iodine. pH susu setelah dipping lebih rendah (P0,05) dari pH susu sebelum dipping. Berdasarkan hasil dapat disimpulkan ekstrak daun babadotan dapat digunakan sebagai alternative antiseptik yang bertindak sebagai anti bakteri dan anti inflamasi dengan konsentrasi terbaik 5% ekstrak. (Effectiveness of Ageratum conyzoides leaves extract as green antiseptic for teat dipping practices in dairy cow) ABSTRACT. The research was conducted to determine the effectiveness babadotan leaf extract (BLE) as an active ingredient of antiseptic teat dip for inhibiting the entry of bacteria through the nipple, and to compare its effectiveness with synthetic antiseptic povidone iodine(PI). Twelve lactating dairycows suffering from subclinical mastitis were used in a completely randomized design. Teat dipping use 5%, 10% and 15% of BLE and PI 10% for 14 days. The result show average total bacterial colonies in milk was reduced significantly (P0.01) from 6,1-7,3log cfu/ml at before dipping to 4,4-6,0 log cfu/ml after dipping. There are no differences between BLE and PI in reducing total bacterial colonies. A decline in the level of the mammary glands inflammation (P0.01) from an average at before teat dipping 6.9 to 4.2 after teat dipping. Compared with PI, the percentage decrease in the level of mammary gland’s inflammation of cows using BLE antiseptic is significantly higher (P0.05). Milk pH at after teat dipping practices is lower (P0.05) than before dipping. In conclusion, BLE can be used as an alternativeantiseptic due to its effectiveness as antibacterial and anti-inflammatory. The recommendation concentration of the babadotan antiseptic is 5% of extract.

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Analisis Efektifitas Tepung Jamur sebagai Larvasida Aedes aegypti

Jurnal Natur Indonesia ◽

10.31258/jnat.14.1.126-130 ◽

2012 ◽

Vol 14 (1) ◽

pp. 126

Author(s):

Yekki Yasmin ◽

Lenni Fitri ◽

Betty Mauliya Bustam

Keyword(s):

Aedes Aegypti ◽

Beauveria Bassiana ◽

Storage Time ◽

Natural Sciences ◽

The Other ◽

Biology Department ◽

Randomized Design ◽

Two Factors ◽

Result Show ◽

Completely Randomized Design

This research is about effectivity analysis of two kinds of fungi i.e. Beauveria bassiana and Metharrizium anisopliae as Aedes aegyptilarvacide, it has been conducted at laboratories Zoology in Biology Department, Mathematics and Natural Sciences Faculty, Syiah KualaUniversity. Aims of this research were estimating maximum time storage of fungi powder as effective larvacide and the number of bacteriacolonies were found in water used for experiment. This research was used Completely Randomized Design (CRD). The experiments consistof two factors i.e kind and saving time of fungi. The result show that the longer the storage time of Beauveria bassiana the more ineffectiveit was as larvacide and the least fungi colonies were found. Metharrizium anisopliae on the other hand, the longer it was kept, the moreeffective it was as larvacide and the more fungi colonies were found.

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PENGARUH LAMA FERMENTASI OLEH Bacillus subtilis TERHADAP KARAKTERISTIK SERE KEDELE

Jurnal Ilmu dan Teknologi Pangan (ITEPA) ◽

10.24843/itepa.2019.v08.i03.p01 ◽

2019 ◽

Vol 8 (3) ◽

pp. 226

Author(s):

Sri Madiarti Sipayung ◽

I Wayan Rai Widarta ◽

I Desak Putu Kartika Pratiwi

Keyword(s):

Bacillus Subtilis ◽

Ph Value ◽

Total Acid ◽

Fermentation Time ◽

Crude Fiber Content ◽

Randomized Design ◽

Result Show ◽

Soybean Fermentation ◽

Completely Randomized Design ◽

Normal Taste

This research aims to find out the effect of soybean fermentation time by Bacillus subtilis to produce sere kedele with the best characteristics. This research used completely randomized design (CRD) with one treatment factor of the fermentation time with 5 period of fermentation time (12, 18, 24, 30 and 36h). Each treatment was repeated 3 times, resulted 15 experimental units. The data were then analyzed with Analysis of Variance method and if the treatment had an effect on the variable, followed by the Duncan test. The result show that fermentation times had significant effect to moisture, ash, protein, fat, carbohydrate, crude fiber content, total acid, pH value, the number microbes, hedonic test for taste, scoring test for taste and overall acceptance of sere kedele. Fermented by B. subtilis for 12h resulted in the best characteristic under the following criteria : 58.05% moisture content, 2.39% ash content, 15.79% protein content, 14.39% fat contetnt, 9,38% carbohydrate content, 6.1 pH value, 0.11% total acid, 9.59log cfu/g the number microbes, color, flavour, texture liked, normal taste with sour taste and overall acceptance liked. Keyword : soybean, sere kedele, Bacillus subtilis, fermentation, traditional food .

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Penggunaan Daging Keong Mas (Pomacea caniculata) terhadap Kualitas Kerupuk

Caraka Tani Journal of Sustainable Agriculture ◽

10.20961/carakatani.v20i2.20502 ◽

2018 ◽

Vol 20 (2) ◽

pp. 80

Author(s):

Choiroel Anam

Keyword(s):

Single Factor ◽

Physical Chemical ◽

Randomized Design ◽

Result Show ◽

Completely Randomized Design ◽

Tapioca Flour

Research about chips made from flour falvored with gold snail has been conducted to know the characteristic of physical, chemical and organoleptic chips results. Data were analyzed with Completely Randomized Design (CRD) by single factor. The factor is ratio between tapioca flour and gold snail meat.The result show that adding snail meat at batter chips was increasing content of water, protein, ash and taste. In other side decreasing content of strach, will reduce the development and color of chips. Chemical, physical and organoleptic examination showed best result at 30% of gold snail meat as substitution at chip.

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Uji Efektivitas Campuran Herbisida Berbahan Aktif Atrazin dan Topramezon terhadap Beberapa Jenis Gulma

JURNAL AGROTROPIKA ◽

10.23960/ja.v20i2.5164 ◽

2021 ◽

Vol 20 (2) ◽

pp. 93

Author(s):

Dad Resiworo Jekti Sembodo ◽

Nana Ratna Wati

Keyword(s):

Survival Model ◽

Active Ingredients ◽

Ageratum Conyzoides ◽

Eleusine Indica ◽

Randomized Design ◽

Herbicide Mixture ◽

Herbicide Atrazine ◽

Completely Randomized Design ◽

Ld50 Value ◽

Synedrella Nodiflora

The purpose of this study was to determine the effectiveness of mixing herbicides with the active ingredients atrazine and topramezone in controlling weeds and to determine the nature of the mixture of the two active ingredients. This research was conducted in a plastic house in Natar District, South Lampung Regency from October 2020 - January 2021. The study was arranged in a Completely Randomized Design (CRD). The treatments consisted of three types of herbicides with six dosage levels of the active ingredients, namely the single herbicide Atrazine 300 g/l (0, 37.5, 75, 150, 300, and 600 g ai ha-1), Topramezon 10 g/l (0. 1.25 , 2.5, 5, 10, and 20 g ai ha-1), and the herbicide mixture of Atrazine 300 g/l + Topramezone 10 g/l (0. 38.75, 77.50, 155, 310, and 620 g ai ha-1) , and repeated 6 times. The target weeds included broadleaf weeds (Ageratum conyzoides and Synedrella nodiflora), grass groups (Digitaria ciliaris, Echinochloa colonum, and Eleusine indica), and the puzzle group (Cyperus iria). The herbicides atrazine and topramezone have different ways of working so that the analytical method used is the Multiplicative Survival Model (MSM) method. The results showed that mixing the herbicide Atrazine 300 g/l + Topramezon 10 g/l had an expected LD50 value of 46.28 g ai ha-1 and a treatment LD50 of 27.22 g ai ha-1 with a co-toxicity value of 1.7 (Co-toxicity > 1) so that it is synergistic.Key words: Atrazin, Topramezon, mixing herbicide, Multiplicative Survival Model, weed, LD50

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Respon Allelopati Gulma Ageratum conyzoides dan Borreria alata terhadap Pertumbuhan dan Hasil Tiga Varietas Kedelai (Glycine max)

Jurnal Agro ◽

10.15575/162 ◽

2015 ◽

Vol 2 (1) ◽

pp. 39-49 ◽

Cited By ~ 1

Author(s):

Abdul Karim Kilkoda

Keyword(s):

Glycine Max ◽

Root Exudates ◽

Crude Extract ◽

Growth And Yield ◽

Independent Effect ◽

Ageratum Conyzoides ◽

Yield Parameters ◽

Randomized Design ◽

Completely Randomized Design ◽

Almost All

Penelitian ini bertujuan untuk menguji pengaruh eksudat akar gulma Ageratum conyzoides dan Borerriaalatayang didalamnya terkandung alelopati tanin dan fenol terhadap pertumbuhan dan hasil tiga varietas kedelai berdasarkan ukuran benih kedelai. Percobaan dilaksanakan di Rumah kaca Fakultas Pertanian Universitas Padjadjaran, JatinangorKabupaten Sumedang, Jawa Baratpada bulan September 2014. Penelitian disusun dalam Rancangan Acak Lengkap factorial. Faktor pertama adalah ekstrak kasar gulma, yaitu; EG0 = ekstrak gulma 0 g/100 ml(kontrol), EG1 = ekstrak gulma (Ageratum + Borreria)100 (g/v), EG2 = ekstrakgulma (Ageratum + Borreria)200 (g/v), EG3 = ekstrak gulma (Ageratum + Borreria) 300 (g/v), faktor kedua yaitu ukuran bobot dan jenis kedelai; K1 = varietas Gepak Kuning (bobot 100 biji : 6,82 gram) ukuran bobot kecil, K2 = varietas Gema(bobot 100 biji : 12 gram) ukuran bobot sedang, K3 = varietas Grobogan (bobot 100 biji : 17,8 gram) ukuran bobot besar. Tidak terdapat interaksi antara ekstrak kasar gulma dengan ukuran bobot dan jenis kedelai terhadap seluruh parameter pertumbuhan dan hasil yang diamati. Terdapat pengaruh mandiri antar sesama varietas kedelai hampir pada semua parameter pertumbuhan dan hasil tanaman kedelai, kecuali parameter luas daun, dengan nilai tertinggi dihasilkan oleh varietas Groboganyang memiliki ukuran biji besar. Terdapat pengaruh mandiri dari ekstrak kasar gulma Ageratum conyzoides dan Borerriaalata pada semua parameter pertumbuhan dan hasil tanaman kedelai.This study aimed to examine the effect of root exudates of weeds Ageratum conyzoides and Borerria alata therein containing tannins and phenols as allelopathy on growth and yield of three soybean varieties with concern to size of the seed. The experiment was conducted in greenhouse of Faculty of Agriculture, Universitas Padjadjaran, Jatinangor Sumedang, West Java in September 2014. The study was set in completely randomized design factorial. First factor was the weed crude extract, namely; EG0 = weed extract 0 g / 100 ml (control), EG1 = weed extract (A. conyzoides + B. alata) 100 (g / v), EG2 = weed extract (A. conyzoides + B. alata) 200 (g / v), EG3 = weed extract (A. conyzoides + B. alata) 300 (g / v). Second factor was and type of soybean; K1 = variety Gepak Kuning (weight of 100 seeds: 6.82 gram) weight size small, K2 = variety Gema (weight of 100 seeds: 12 gram) weight size medium, K3 = variety Grobogan (weight of 100 seeds: 17.8 gram) weight size big. There was no interaction between weed crude extract and soybean type. Meanwhile, there was independent effect among varieties tested for almost all parameters observed, except for leaf width. There was also independent effect from crude extract of Ageratum conyzoides and Borerria alata on all growth and yield parameters of soybean.RESPON ALLELOPATI GULMA Ageratum conyzoides DAN Borreria alata TERHADAPPERTUMBUHAN DAN HASIL TIGA VARIETAS KEDELAI (Glycine max)<w:LsdException Locked="false" Priority="70" SemiHidden="false" UnhideWhenUsed="false" Name="Dark List

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UNTILIZATION OF Moringa oleifera LEAF EXTRACT ON DECREASING INFESTATION OF Argulus japonicus IN GOLDFISH (Carassius auratus)

AQUASAINS ◽

10.23960/aqs.v8i1.p769-774 ◽

2019 ◽

Vol 8 (1) ◽

pp. 769

Author(s):

Febri Setyawati ◽

Kismiyati Kismiyati ◽

Sri Subekti

Keyword(s):

Carassius Auratus ◽

Leaf Extract ◽

Moringa Oleifera ◽

Optimal Concentration ◽

Percentage Decrease ◽

Goldfish Carassius Auratus ◽

Randomized Design ◽

Completely Randomized Design ◽

Range Test ◽

Moringa Leaf Extract

Abstrak Goldfish (Carassius auratus) is one of the fishes that can be infected by parasites. Commonly, this fish is often attacked by Argulus japonicus ectoparasites. Control of infestation in A. japonicus can be done by utilizing one of the natural ingredients, Moringa oleifera leaf extract, which contains of alkaloids, tannins, saponins and flavonoids. This study aims to determine the effect of M. oleifera leaf extract in reducing the infestation of A. japonicus and the optimal concentration of M. oleifera leaf extract in reducing the infestation of A. japonicus in goldfish (C. auratus). This study used a completely randomized design (CRD) with treatments namely control, 600 ppm, 700 ppm, 800 ppm and 900 ppm with four times of replication. The main parameter is the percentage decrease in infestation of A. japonicus. The results of the research data were analyzed by using ANOVA and followed with the Ducan Multiple Range Test. The results showed that Moringa leaf extract could reduce the infestation of A. japonicus. The optimal concentration of Moringa leaf extract in reducing the infestation of A. japonicus was obtained from a concentration of 700 ppm.

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Prevalence of mastitis in dairy goat farms in Eastern Algeria

Veterinary World ◽

10.14202/vetworld.2019.1563-1572 ◽

2019 ◽

Vol 12 (10) ◽

pp. 1563-1572 ◽

Cited By ~ 1

Author(s):

Zahra Gabli ◽

Zouhir Djerrou ◽

Abd Elhafid Gabli ◽

Mounira Bensalem

Keyword(s):

Subclinical Mastitis ◽

Mammary Glands ◽

Clinical Mastitis ◽

Control Measures ◽

Dairy Goat ◽

Coagulase Negative Staphylococci ◽

California Mastitis Test ◽

Eastern Algeria ◽

And Control ◽

Positive Half

Aim: This study aimed to investigate mastitis in dairy goat farms through the California mastitis test (CMT) and bacteriological examinations. Materials and Methods: A total of 845 goats belonging to 18 farms from four regions (Tébessa, Guelma, Souk Ahras, and Skikda) were examined. Results: Clinical examination of the mammary glands showed that 30/845 (3.55%) goats had clinical mastitis and 32 goats had half-teat inflammation. CMT subclinical mastitis (SCM) was detected in 815 goats that were presumed to be healthy. CMT showed 46 (5.64%) CMT-positive goats as well as 47 (2.88%) positive half-udders with a score of ≥2. A total of 79 bacteria were isolated and identified from the 79 bacterial positive samples. Bacteriological analyses showed that Gram-positive staphylococci were largely responsible for clinical and SCM. Coagulase-negative staphylococci, with an isolation frequency of 56.96%, were the most prevalent bacteria from all isolated organisms. The second most prevalent organism was Staphylococcus aureus at 40.50% and streptococci (2.53%) had the smallest percentage of isolation. Conclusion: It is suggested that due to the prevalence of mastitis in this species, farmers should be aware of the problem to plan preventive and control measures to reduce dairy goat losses due to this disease.

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The prevalence of subclinical mastitis in dairy goats in Kenya

Journal of the South African Veterinary Association ◽

10.4102/jsava.v71i1.672 ◽

2000 ◽

Vol 71 (1) ◽

Cited By ~ 2

Author(s):

E.N. Ndegwa ◽

C.M. Mulei ◽

S.J.M. Munyua

Keyword(s):

Subclinical Mastitis ◽

Mammary Glands ◽

Dairy Goats ◽

Bacterial Isolation ◽

Milk Samples ◽

California Mastitis Test ◽

Cell Counts ◽

Mere Presence ◽

Leukocyte Counts ◽

Apparently Healthy

California mastitis test (CMT), direct leukocytes counts and bacteriological examination were performed on 630 milk samples from apparently healthy mammary glands of dairy goats comprising a mixed population of German Alpine, Toggenberg, Saanen and Galla crosses to find the prevalence of subclinical mastitis. The prevalence of subclinical mastitis was 9.8 %according to CMT, 9.7 %according to direct leukocyte counts and 28.7 %by bacterial isolation during a 3-month period. The proportion of the bacteriologically positive milk samples was significantly (P < 0.01) higher than that positive for CMTand direct leukocyte counts. There was a significant (P < 0.01) correlation between CMT and direct leukocyte counts. There was no significant direct relationship between bacterial isolation and CMT. Bacterial organisms were isolated in 22.5 % of the 568 CMT-negative milk samples. The results suggest that bacterial organisms isolated from the CMT-negative milk samples were either latent infections or did not stimulate any significant increase in somatic cell counts that could be detected by either the CMT or direct leukocyte counts. The observations of this study indicate that the mere presence of bacteria in goat's milk does not mean that the udder is infected and so does not warrant antibiotic therapy.

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Peningkatan Kualitas Jerami Padi MenggunakanTeknologi Amoniasi Fermentasi

Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology ◽

10.29303/jitpi.v2i1.18 ◽

2019 ◽

Vol 2 (1) ◽

pp. 96

Author(s):

Muhamad Amin ◽

Sofyan Damrah Hasan ◽

Oscar Yanuarianto ◽

Mohammad Iqbal ◽

I Wayan Karda

Keyword(s):

Organic Matter ◽

Crude Protein ◽

Dry Matter ◽

Crude Fiber ◽

In Vitro Digestibility ◽

Randomized Design ◽

Result Show ◽

Completely Randomized Design ◽

Range Test

An experiment with aim to study the effect of probiotics combination level and length of fermentation on qualityimprovement of rice straw had been conducted at the Laboratory of Nutrition and Feed of Animal HusbandryFaculty, Mataram University. The research was arranged in a Completely Randomized Design in factorialarrangement 3 x 3 with 3 replications. The first factor was the level of probiotics (0, 2 and 4%), the second factorwas the period of fermentation (2, 4 and 6 weeks).The variables measured were crude protein, crude fiber, NDF,ADF, cellulose, hemicellulose, lignin, and in vitro digestibility of dry matter and organic matter. Data wereanalyzed by analysis of variance and tested further by Duncan's Multiple Range Test. The result show thattreatments combination of the probiotics level and fermentation period did not affect (P>0.05) the chemicalcomposition and digestibility. The level of probiotics increased (P<0.05) the crude protein and in vitro dry matterdigestibility. Meanwhile, the lenght of fermentation increased the crude protein and in vitro digestibility of drymatter and organic matter, but decreased the crude fiber, NDF, ADF, cellulose, and hemicellulose of Amofer ricestraw.

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EFFECT OF NONI FRUIT (Morinda citrifolia) POWDER AS FEED ADDITIVE ON INTESTINAL MICROFLORA AND VILI CHARACTERISTICS OF HYBRID DUCK

Buletin Peternakan ◽

10.21059/buletinpeternak.v40i1.8677 ◽

2016 ◽

Vol 40 (1) ◽

pp. 33 ◽

Cited By ~ 2

Author(s):

David Kurniawan ◽

Eko Widodo ◽

Irfan H Djunaidi

Keyword(s):

Intestinal Microflora ◽

Positive Control ◽

Negative Control ◽

Morinda Citrifolia ◽

Feed Additive ◽

Randomized Design ◽

Result Show ◽

Completely Randomized Design ◽

Fruit Powder ◽

Range Test

Noni fruit is a medicanal plant with biological activity like antimicrobial and antioxidant that could potentially be used as a feed additive in poultry. This research investigated the effect of noni fruit powder as feed additive on intestinal microflora and villi characteristics of hybrid duck. The subjects for this study were one hundred fifty of 2-week-old hybrid duck that were divided into 5 dietary groups, namely P0: basal feed serving as negative control, P1: basal feed + 1 % noni fruit powder, P2: basal feed + 2 % noni fruit powder, P3: basal feed + 3 % noni fruit powder, P4: basal feed supplemented with tetracycline antibiotic (300mg/kg feed) as positive control. Data were analyzed by one-way of Completely Randomized Design and if there was significant effect followed by Duncan’s Multiple Range Test. The result show that levels of noni fruit powder significantly influence (P<0.01) intestinal microflora. Total Escherichia coli of intestinal microflora was decreasing, however total Lactic acid bacteria was equal to negative control. Intestinal villi characteristics show that significantly different (P<0.01) with used noni fruit powder. Treatment noni fruit powder 1% can increase villus length and villus surface area. (Key words: Duck, Intestinal changes, Noni Fruit, Phytobiotic)

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