scholarly journals The influence of calyx maturation stage and types of packaging on the germination of goldenberry seeds

2020 ◽  
Vol 14 ◽  
Author(s):  
Weslley Rosário Santana ◽  
Érica Duarte Silva ◽  
Gleison Oliosi ◽  
Jalille Amim Altoé ◽  
Jeniffer Ribeiro De Oliveira ◽  
...  
Keyword(s):  
Seed Viability ◽  
Storage Period ◽  
Maturation Stage ◽  
Harvest Time ◽  
Germination Test ◽  
Brownish Yellow ◽  
Greenish Yellow ◽  
Plastic Bags ◽  
Maturation Stages ◽  
Sexual Propagation

Studies of the optimum harvest time for obtaining seeds for the sexual propagation of the goldenberry are essential to help maintain seed viability. This study aimed to determine the optimum harvest time for the goldenberry based on the calyx maturation stage and viability of stored seeds. The design was completely randomized a in factorial design (5 x 3), with three replications. The treatments consisted of a combination of five calyx maturation stages, assessed based on color (green, green-yellow, greenish-yellow, yellow, brownishyellow), and three types of packaging: Kraft paper bags, plastic bags, and glass. The seeds were removed from the fruit and stored at the same temperature and shade conditions for 120 days. Based on the results of the 21-day germination test, it is recommended that goldenberry seeds should be obtained from fruit with a green-yellow to brownish-yellow calyx. All the packaging types analyzed by this study are recommended for the storage of goldenberry seeds obtained from fruit with a green-yellow to brownish-yellow calyx for a 120-day storage period.

scholarly journals Physiological potential of soybean seeds after maturation and submitted to artificial drying

2017 ◽  
Vol 39 (4) ◽  
pp. 374-384
Author(s):  
Cesar Pedro Hartmann Filho ◽  
André Luís Duarte Goneli ◽  
Tathiana Elisa Masetto ◽  
Elton Aparecido Siqueira Martins ◽  
Guilherme Cardoso Oba
Keyword(s):  
Moisture Content ◽  
Dry Matter ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Maturation Stage ◽  
Soybean Seeds ◽  
Germination Test ◽  
Moisture Contents ◽  
Three Samples ◽  
Maturation Stages

Abstract: This study evaluated the physiological potential of soybean seeds harvested during two seasons, on different maturation stages and subjected to different drying temperatures. The seeds were harvested at the maturations stages R7, R7 + 2, R7 + 3, R7 + 5, R7 + 6, R7 + 7, R7 + 10 and R7 + 12 days (55, 50, 45, 40, 35, 30, 25, and 20% of moisture content). For each maturation stage, seeds were divided into three samples: one sample was used to directly evaluate the physiological potential, and the others were dried at 40 °C and 50 °C, until reaching the moisture content of 11.5%. The physiological potential was evaluated through germination test, first germination count of germination, accelerated aging, modified cold, electrical conductivity and seedling emergence. The maximum physiological potential of seeds is achieved at the moisture content of 55%, the point that the dry matter is maximum. The seeds became tolerant to artificial drying approximately at the stage R7 + 7 days (30% of moisture content). Germination and vigor of the soybean seeds reduce as the drying temperature is increased from 40 °C to 50 °C, and this effect is enhanced when the seeds show moisture contents above 30%.

scholarly journals Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

2014 ◽  
Vol 36 (3) ◽  
pp. 344-351 ◽  
Author(s):  
Daiana Karoline Kaiser ◽  
Laura Cristiane Nascimento de Freitas ◽  
Rubia Priscila Biron ◽  
Simone Cristina Simonato ◽  
Michele Fernanda Bortolini
Keyword(s):  
Seed Viability ◽  
Germination Test ◽  
Preparation Methods ◽  
Eugenia Uniflora ◽  
Paper Towel ◽  
Plastic Bags ◽  
Longitudinal Cutting ◽  
Direct Immersion ◽  
Tetrazolium Test

The study aimed to adjust the methodology of the tetrazolium test to estimate seed viability of Eugenia uniflora L. (Surinam Cherry). Seeds were collected in September 2012 and divided into four lots: freshly harvested (Lot I); stored in plastic bags in a refrigerator at 10 °C for: 15 days (Lot II); 30 days (Lot III); and 45 days (Lot IV). The freshly harvested seeds were preconditioned with direct immersion in water and wet paper towel, followed or not by longitudinal cutting. The seeds were immersed in a 0.1% tetrazolium solution for 4 hours at 30 °C. Appropriate soaking and preparation methods were applied to the seeds lots using three tetrazolium concentrations: 0.1; 0.5; and 1.0%; and four preconditioning periods (2, 4, 6 and 8 hours) at 30 °C. The viability results obtained by the tetrazolium test were compared with those of the germination test. Direct immersion of seeds in water for 24 hours at 25 °C, followed by a longitudinal cut was efficient for preconditioning the seeds. Seed staining with tetrazolium solution at a concentration of 0.5% for 2 hours at 30 °C can be used to estimate the viability of freshly harvested and stored Surinam Cherry seeds.

scholarly journals CONSERVATION OF SEEDS OF Myrcianthes pungens (Berg) Legr. IN DIFFERENT PACKAGING IN A CONTROLLED ENVIRONMENT1

2019 ◽  
Vol 43 (2) ◽  
Author(s):  
Patrícia Gibbert ◽  
Marlene de Matos Malavasi ◽  
Ubirajara Contro Malavasi ◽  
João Alexandre Lopes Dranski
Keyword(s):  
Water Content ◽  
Dry Matter ◽  
Storage Period ◽  
Germination Test ◽  
Plastic Packaging ◽  
Glass Containers ◽  
Plastic Bags ◽  
Seedling Length ◽  
Plastic Containers ◽  
Physiological And Biochemical

ABSTRACT Studying the physiological and biochemical behavior of Myrcianthes pungens seeds stored in different packages for up to ten months was the objective of this work. Seeds were collected in Toledo, Pato Bragado, and Marechal Cândido Rondon and stored in a cold and dry chamber (11 ºC and 6,3% RH) for ten months. The germination test and the germination speed index (GSI), the seedling length (SL), the seedling dry matter mass (DMS), and the tetrazolium (ZT) test were performed and, for each evaluation, the water content was determined. Non-parametric analysis was used. During storage, the water content of the seeds in the plastic and glass containers varied little, but decreased in the paper packaging. The germination was maintained for ten months when the seeds were stored in plastic containers and for two months in the glass and paper ones. The variables GSI, SL, and DMS presented upwards and downwards variations during the storage period. The ZT revealed that, in the plastic packaging, the seeds remained viable for up to ten months, in glass, up to two months, and in paper, up to four months. Therefore, seeds of Myrcianthes pungens with high initial quality can be stored in a cold and dry chamber in plastic bags, maintaining viability and vigor for a period of ten months

Variations in germination and longevity of pepper (Capsicum annuum L.) seeds harvested at different stages of maturation

2002 ◽  
Vol 50 (2) ◽  
pp. 157-162
Author(s):  
J. A. Oladiran ◽  
P. A. Kortse
Keyword(s):  
Capsicum Annuum ◽  
Seed Weight ◽  
Seed Viability ◽  
Storage Period ◽  
Dry Weight ◽  
Mother Plant ◽  
Fruit Maturation ◽  
Capsicum Annuum L ◽  
Maturation Stages ◽  
Days After Anthesis

Pepper (Capsicum annuum L.) seeds of the cultivars Tatashe and Rodo, extracted from fruits sequentially harvested at different maturation stages, were tested for germinability (when freshly harvested) and longevity (over a period of about 17 weeks). Seed dry weight increased with fruit maturation and was maximum when the fruits turned red-ripe. Over-ripening of the fruits for 10 days on the mother plant did not result in a significant seed weight increase, signifying that the seeds were already fully filled when the fruits turned red-ripe. The seeds of the two cultivars started germinating as from 28 days after anthesis (DAA) and peaked at 71.5% and 40% for Tatashe and Rodo respectively, at 44 DAA. The viability of the seeds of Tatashe declined as storage progressed and the more mature the seeds were, the longer they survived. In Rodo, seed viability increased till the end of the storage period due to dormancy, which became progressively broken with age; seeds from more mature fruits survived better. In a second experiment, when fruits of Tatashe were harvested at the colour breaking, red-ripe and over-ripe stages, seed viability and longevity were best from over-ripe fruits.

scholarly journals Seed viability changes during fruit ripening of Tapirira guianensis Aubl.: Implications for collection

2020 ◽  
Vol 9 (11) ◽  
pp. e3459119719
Author(s):  
Lorene Bianca Araújo Tadaiesky ◽  
Dênmora Gomes de Araújo ◽  
Tainah Kaylla dos Santos Aquino ◽  
Saulo Fabrício da Silva Chaves ◽  
Elson Junior Souza da Silva
Keyword(s):  
Environmental Changes ◽  
Seed Viability ◽  
Fruit Production ◽  
Maturation Stage ◽  
Fruit Maturation ◽  
Seed Collection ◽  
Parent Tree ◽  
Randomized Design ◽  
Maturation Stages ◽  
External Characteristics

Environmental changes provoked by man have caused great impacts on nature with irreparable consequences to the environment, life, and biodiversity. Species as Tapirira guianensis that have high ecological potential, due to adaptability, fast growth, and abundant fruit production, can contribute to the restoration of these environments. The objective of this work was to verify the influence of the fruit maturation stage on the physiological potential of the Tapirira guianensis seeds from three genitor plants and, thus, to define seed collection patterns based on the fruit external characteristics. The experiment was performed in a completely randomized design, in factorial scheme 3 x 3, three parent trees (1, 2, and 3) and three fruit maturation stages (green, intermediate and mature fruits), containing 4 replicates of 25 seeds per treatment. Seeds from mature fruits in comparison to those of green fruits presented significant reductions in the water content in the parent trees 1, 2 e 3 de 11%, 21%, and 28%, respectively. Otherwise, seeds from mature fruits presented significant increases in the emergence and emergence speed index of 7% and 11% for parent tree 1, 76%, and 108% for parent tree 2, and 40% and 40% for parent tree 3, in comparison to the seeds from green fruits. This research revealed that was possible to define physiological maturation of seeds according to the fruit maturation stages, being the seeds from intermediate and mature fruits that present higher germination power and development of seedlings and, therefore, indicated for collection of Tapirira guianensis seeds.

scholarly journals Desiccation Tolerance as the Basis of Long-Term Seed Viability

2020 ◽  
Vol 22 (1) ◽  
pp. 101
Author(s):  
Galina Smolikova ◽  
Tatiana Leonova ◽  
Natalia Vashurina ◽  
Andrej Frolov ◽  
Sergei Medvedev
Keyword(s):  
Desiccation Tolerance ◽  
Vascular Plants ◽  
Molecular Mechanisms ◽  
Regulation Of Gene Expression ◽  
Seed Viability ◽  
Late Embryogenesis Abundant ◽  
Maturation Stage ◽  
Terrestrial Plants ◽  
Complex Anatomy ◽  
Orthodox Seeds

Desiccation tolerance appeared as the key adaptation feature of photoautotrophic organisms for survival in terrestrial habitats. During the further evolution, vascular plants developed complex anatomy structures and molecular mechanisms to maintain the hydrated state of cell environment and sustain dehydration. However, the role of the genes encoding the mechanisms behind this adaptive feature of terrestrial plants changed with their evolution. Thus, in higher vascular plants it is restricted to protection of spores, seeds and pollen from dehydration, whereas the mature vegetative stages became sensitive to desiccation. During maturation, orthodox seeds lose up to 95% of water and successfully enter dormancy. This feature allows seeds maintaining their viability even under strongly fluctuating environmental conditions. The mechanisms behind the desiccation tolerance are activated at the late seed maturation stage and are associated with the accumulation of late embryogenesis abundant (LEA) proteins, small heat shock proteins (sHSP), non-reducing oligosaccharides, and antioxidants of different chemical nature. The main regulators of maturation and desiccation tolerance are abscisic acid and protein DOG1, which control the network of transcription factors, represented by LEC1, LEC2, FUS3, ABI3, ABI5, AGL67, PLATZ1, PLATZ2. This network is complemented by epigenetic regulation of gene expression via methylation of DNA, post-translational modifications of histones and chromatin remodeling. These fine regulatory mechanisms allow orthodox seeds maintaining desiccation tolerance during the whole period of germination up to the stage of radicle protrusion. This time point, in which seeds lose desiccation tolerance, is critical for the whole process of seed development.

scholarly journals Physiological quality of Gliricidia sepium (Jacq.) Steud. (Leguminosae - Papilionoideae) seeds subjected to different storage conditions

2012 ◽  
Vol 36 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Renata Conduru Ribeiro Reis ◽  
Claudinéia Regina Pelacani ◽  
Cimille Gabrielle Cardoso Antunes ◽  
Bárbara França Dantas ◽  
Renato Delmondez de Castro
Keyword(s):  
Water Content ◽  
Seed Viability ◽  
Gliricidia Sepium ◽  
Storage Conditions ◽  
Physiological Processes ◽  
Plastic Bags ◽  
Starting Point ◽  
Cultivated Plant ◽  
Physiological Quality

Studies of seed physiological processes represent the starting point for the sustainable utilization of native or cultivated plant species from the caatinga biome for which germination studies are still scarce. In order to determine adequate methods for the conservation of Gliricidia sepium seeds, the physiological quality of propagules exposed to different conditions and times of storage were examined. Recently collected seeds of G. sepium were analyzed immediately, while the other sample was stored for three, six, nine, or twelve months in plastic or paper bags under refrigeration or at room temperature for further analysis. The germination rates of seeds from each storage regime were determined and these rates compared to their water content. It was observed that germination capacity was directly related to changes in seed humidity. Storing seeds in plastic bags yielded the best conservation, mainly when they were stored at low temperatures. However, even at temperatures near 25°C it was possible to prolong seed viability when their water content was controlled through the use of impermeable packing.

Ageing in embryos from wheat grains stored at different temperatures: oxidative stress and antioxidant response

2011 ◽  
Vol 38 (7) ◽  
pp. 624 ◽  
Author(s):  
Carmelina Spanò ◽  
Stefania Bottega ◽  
Roberto Lorenzi ◽  
Isa Grilli
Keyword(s):  
Oxidative Stress ◽  
Low Temperature ◽  
Seed Viability ◽  
Storage Period ◽  
Antioxidant Response ◽  
Protective Role ◽  
Native Page ◽  
Wheat Grains ◽  
Different Temperatures ◽  
Enzymatic Machinery

In the present work we studied oxidative stress as an important cause of seed deterioration during ageing in embryos from durum wheat grains stored at room temperature and at low temperature (10°C). The protective role of low temperature on seed viability was confirmed. The increase of hydrogen peroxide content during dry storage was strongly correlated with the decrease of germinability. Ascorbate and glutathione showed a good correlation with grain germinability and significantly increased upon imbibition, in particular in embryos from viable grains. Ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione peroxidase (GPX) and catalase (CAT) were studied quantitatively (enzymatic assays). APX, GR, and GPX were also studied qualitatively by native PAGE. The enzymes were active in dry, still viable, embryos whereas no activity was detected in non-viable embryos. With the exception of APX, all enzymatic activities decreased upon imbibition. The study of grains stored in different conditions indicated a negative correlation between the efficiency of the antioxidant enzymatic machinery and the age of the grain. The differences detected in differently stored materials confirmed that both germination parameters and the length of storage period are important in determining grain condition.

scholarly journals Successional stages in infant gut microbiota maturation

2021 ◽  
Author(s):  
Leen Beller ◽  
Ward Deboutte ◽  
Gwen Falony ◽  
Sara Vieira Silva ◽  
Raul Tito ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Early Life ◽  
Microbial Colonization ◽  
Maturation Stage ◽  
Gut Flora ◽  
Microbiome Composition ◽  
Gut Colonization ◽  
Maturation Stages ◽  
Colonization Process ◽  
First Year Of Life

Background: Disturbances in the primary colonization of the infant gut can result in life-long consequences and have been associated with a range of host conditions. Although early life factors have been shown to affect the infant gut microbiota development, our current understanding of the human gut colonization in early life remains limited. To gain more insights in the unique dynamics of this rapidly evolving ecosystem, we investigated the microbiota over the first year of life in eight densely sampled infants (total number of samples, n=303). To evaluate gut microbiota maturation transition towards an adult configuration, we compared the microbiome composition of the infants to the Flemish Gut Flora Project population (n=1,106). Results: We observed the infant gut microbiota to mature through three distinct, conserved stages of ecosystem development. Across these successional gut microbiota maturation stages, genus predominance was observed to shift from Escherichia over Bifidobacterium to Bacteroides. Both disease and antibiotic treatment were observed to be associated occasionally with gut microbiota maturation stage regression, a transient setback in microbiota maturation dynamics. Although the studied microbiota trajectories evolved to more adult-like constellations, microbiome community typing against the background of the Flemish Gut Flora Project (FGFP) cohort clustered all infant samples within the (in adults) potentially dysbiotic Bact2 enterotype. Conclusion: We confirmed similarities between infant gut microbial colonization and adult dysbiosis. A profound knowledge about the primary gut colonization process in infants might provide crucial insights into how the secondary colonization of a dysbiotic adult gut can be redirected.

scholarly journals Comparative Proteomics at the Critical Node of Vigor Loss in Wheat Seeds Differing in Storability

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiuling Chen ◽  
Andreas Börner ◽  
Xia Xin ◽  
Manuela Nagel ◽  
Juanjuan He ◽  
...  
Keyword(s):  
Seed Viability ◽  
Storage Period ◽  
Protein Translation ◽  
Artificial Ageing ◽  
Plateau Phase ◽  
Cell Defense ◽  
Critical Node ◽  
Cytotoxic Compounds ◽  
Wheat Seeds

The critical node (CN, 85% germination) of seed viability is an important threshold for seed regeneration decisions after long-term conservation. Dependent on the germplasm, the storage period until CN is reached varies and information on the divergence of the proteomic profiles is limited. Therefore, the study aims to identify key proteins and mechanisms relevant for a long plateau phase and a late CN during artificial seed aging of wheat. Seeds of the storage-tolerant genotype (ST) TRI 23248, and the storage-sensitive genotype (SS) TRI 10230 were exposed to artificial ageing (AA) and extracted embryos of imbibed seeds were analyzed using an iTRAQ-based proteomic technique. ST and SS required AA for 24 and 18 days to reach the CN, respectively. Fifty-seven and 165 differentially abundant proteins (DAPs) were observed in the control and aged groups, respectively. Interestingly, a higher activity in metabolic processes, protein synthesis, transcription, cell growth/division, and signal transduction were already found in imbibed embryos of control ST seeds. After AA, 132 and 64 DAPs were accumulated in imbibed embryos of both aged ST and SS seeds, respectively, which were mainly associated with cell defense, rescue, and metabolism. Moreover, 78 DAPs of ST appeared before CN and were mainly enriched in biological pathways related to the maintenance of redox and carbon homeostasis and they presented a stronger protein translation ability. In contrast, in SS, only 3 DAPs appeared before CN and were enriched only in the structural constituents of the cytoskeleton. In conclusion, a longer span of plateau phase might be obtained in seeds when proteins indicate an intense stress response before CN and include the effective maintenance of cellular homeostasis, and avoidance of excess accumulation of cytotoxic compounds. Although key proteins, inherent factors and the precise regulatory mechanisms need to be further investigated, the found proteins may also have functional potential roles during long-term seed conservation.

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