scholarly journals APOPTOSIS RED NUCLEUS NEURONS OF PARKINSON DISEASE RAT MODEL INDUCED BY PARAQUAT DICHLORIDE

2020 ◽  
Vol 21 (4) ◽  
pp. 588-595
Author(s):  
Ayu Miftahul Khasanah ◽  
Niswah Nurul Fahma ◽  
Tri Wahyu Pangestiningsih
Keyword(s):  
Cell Death ◽  
Treatment Group ◽  
Parkinson Disease ◽  
Rat Model ◽  
Red Nucleus ◽  
Motor Impairment ◽  
The Body ◽  
Control Group ◽  
Male Wistar Rats ◽  
Programed Cell Death

Red nucleus plays a role in motoric control, so that disturbanced of neurons in this nucleus could be affected on motor impairment. Previous study reported that paraquat (PQ) could induced Parkinson disease, a neurodegeneratif disease that affected subtantia nigra pars compactca and clinically characterized by motoric disorders. Apoptosis is a programed cell death that physiologically normal for the body, but in large quantities will cause progresive cell death. This study aimed to observed apoptosis in rats RN neurons following by PQ injection. Ten male Wistar rats with the aged of 3 month, devided into two groups of five. Control group was given aquadest and treatment group was given PQ, injected intraperitoneally, dosage 7 mg/kg BW, twice a week for three weeks. At day 24th all rats were anesthetized using ketamin dosage 40 mg/kg BW and xylazine dosage 5 mg/kg BW, perfused intracardially using 4% paraformaldehyde and mesencephalons were collected. Mesencephalons were processed for histological preparations using the paraffin method and cut stereologically in coronal section, in 4 ?m thickness. Immunohistochemistry staining was done using caspase 3 antibody as a marker of apoptosis. Data were analyzed descriptively and quantitatively. The results in both groups showed RN neurons were large size and stelat or fusiform in shape. The percentage of apoptotic neurons in the RN of treatment group was significantly increased (80,4%±13,8%) compared to the control group (26,6%±18,32%) (P<0.01). In conclusion, following PQ exposure there is a significant increased of apoptosis neurons number in the RN of rat model of Parkinson disease.

Perbandingan Efek Suplementasi Tablet Tambah Darah Dengan Dan Tanpa Vitamin C Terhadap Kadar Hemoglobin Pada Ibu Hamil Dengan Usiakehamilan 16-32 Minggu Di Desa Keniten Kecamatan Mojo Kabupaten Kediri

2017 ◽  
Vol 3 (1) ◽  
pp. 76
Author(s):  
Siti Asiyah ◽  
Dwi Estuning Rahayu ◽  
Wiranti Dwi Novita Isnaeni
Keyword(s):  
Treatment Group ◽  
Vitamin C ◽  
Gestational Age ◽  
Group Treatment ◽  
Sampling Technique ◽  
Hemoglobin Level ◽  
The Body ◽  
Control Group ◽  
Fetal Organ ◽  
And Control

The needed of Iron Tablet in pregnancy was increase than mother who not pregnant.  That  cause of  high metabolism at the pregnancy for formed of  fetal organ and energy. One of effort for prevent anemia in mother pregnant with giving the Iron tablet and vitamin c. The reason of  this research in 4 June – 11 July 2014 is for compare the effect of  iron tablet suplementation with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks In Desa Keniten Kecamatan Mojo Kabupaten Kediri. This research method using comparative analytical.  Research design type of Quasy Eksperiment that have treatment group and control group. Treatment group will giving by Iron tablet and 100 mg vitamin C, and control group just giving by iron tablet during 21 days. Population in this research are all of mother pregnant with Gestational Age Of 16-32 Weeks with Sampling technique is  cluster random sampling is 29 mother pregnant. Comparison analysis of  iron tablet suplementation effect with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks, data analysis using Mann Whitney U-test and the calculated U value (44,5) less than U-table (51). So there was difference of iron tablet suplementation effect with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks Therefore, the addition of vitamin C on iron intake is needed to increase the uptake of iron tablets. When the amount of iron uptake increases, the reserves of iron in the body will also increase, so as to prevent anemia in pregnant women; Keywords : Iron Tablet (Fe), Vitamin C, Hemoglobin level, Mother Pregnant

Effect of High Plane of Nutrition on Blood Biochemical Profile and Onset of Puberty in Prepubertal HF X Kankrej Crossbred Heifers

2019 ◽  
Vol 15 (02) ◽  
pp. 14-17
Author(s):  
K K Hadiya ◽  
A J Dhami ◽  
D V Chaudhari ◽  
P M Lunagariya
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Plasma Levels ◽  
Age Of Onset ◽  
The Body ◽  
Monthly Interval ◽  
Control Group ◽  
Blood Biochemical ◽  
Lower Protein ◽  
The Mean

This study was initiated on 24 prepubertal Holstein x Kankrej crossbred heifers of nearly identical age (7-9 months) and body weight (130-140 kg) at University farm to evaluate the effect of high plane of nutrition on blood biochemical and minerals profile and the age at puberty. Twelve heifers were managed under routine farm feeding (control) and the rest 12 under ideal optimum feeding regime (treatment) that included extra 1 kg concentrate, 30 g min mix and ad-lib dry fodder. The body weight and ovarian ultrasonography together with blood sampling was carried out at monthly interval from 10 to 18 months of age to study the ovarian dynamics and blood biochemical changes. High plane of nutrition to growing heifers was beneficial in reducing the age of onset of puberty (by 2-3 months) compared to routine farm fed group. The mean plasma total protein and cholesterol concentrations showed a rising trend with significant variations from 10 to 16 months of age, where it got mostly stabilized indicating adult profile. The activity of enzymes GOT and GPT also rose gradually and significantly from 10 months till 14-15 months of age, and thereafter it remained more or less static till 18 months of age. The levels of both these enzymes were higher, with lower protein and cholesterol, in control than the treatment group from 15-16 months of age onwards. The mean plasma levels of both calcium and phosphorus increased gradually and significantly with advancing age till 16-17 months of age, with little higher values in supplemented than a control group. The plasma levels of zinc, iron, copper, and cobalt also showed rising trend with significant differences between 10th and 12th-14th months of age, and from 15th to 18th months of age the levels were statistically the same in all the groups with slightly higher values in the treatment group.

scholarly journals Impact of Vanadium Complexes Treatment on the Oxidative Stress Factors in Wistar Rats Plasma

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
R. Francik ◽  
M. Krośniak ◽  
M. Barlik ◽  
A. Kudła ◽  
R. Gryboś ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Reduced Glutathione ◽  
The Body ◽  
Stress Factors ◽  
Control Group ◽  
Vanadium Complexes ◽  
Healthy Male ◽  
Reducing Ability ◽  
Male Wistar Rats ◽  
Antioxidant Parameters

The aim of this study was to investigate the clinical efficacy of vanadium complexes on triglycerides (TG), total cholesterol (Chol), uric acid (UA), urea (U), and antioxidant parameters: nonenzymatic (FRAP—ferric reducing ability of plasma, and reduced glutathione—GSH) and enzymatic (glutathione peroxidase—GPx, catalase—CAT, and GPx/CAT ratio) activity in the plasma of healthy male Wistar rats. Three vanadium complexes: [VO(bpy)2]SO4⋅2H2O, [VO(4,4′Me2bpy)2]SO4⋅2H2O, and Na[VO(O2)2(bpy)]⋅8H2O are administered by gavage during 5 weeks in two different diets such as control (C) and high fatty (F) diets. Changes of biochemical and antioxidants parameters are measured in plasma. All three vanadium complexes statistically decrease the body mass growth in comparison to the control and fatty diet. In plasma GSH was statistically increased in all vanadium complexes-treated rats from control and fatty group in comparison to only control group. Calculated GPX/CAT ratio was the highest in the control group in comparison to others.

Effect Of Pregnancy Exercise On Third Trimester Primigravida Anxiety In Dealing With Childbirth

2021 ◽  
pp. 76-83
Author(s):  
Ismi Puji Astuti
Keyword(s):  
Treatment Group ◽  
Sampling Technique ◽  
The Body ◽  
Rank Test ◽  
Control Group ◽  
P Value ◽  
Research Subjects ◽  
Third Trimester ◽  
Quasi Experimental ◽  
Signed Rank Test

Background: The first labor anxiety is an unpleasant psychological condition due to physiological changes that cause psychological instability. The condition of excessive anxiety, worry, fear without cause, and stress, causes the muscles of the body to tense up, especially the muscles in the birth path to become stiff and hard, making it difficult to expand. This study analyzed the effect of pregnancy exercise on third trimester primigravida anxiety in dealing with childbirth. Methods: Quasi experimental design with nonrandomized pretest-postest control group. The sampling technique used was purposive sampling with 34 research subjects. 17 people for the treatment group were given pregnancy exercise 8 times, and 17 people for the control group were given health education about preparation for childbirth. Results: The results of data analysis using the Wilcoxon signed rank test showed that the average reduction in the treatment group was 8.00 and the control group was 3.00. The effect of pregnancy exercise on third trimester primigravida anxiety in the treatment group with a p-value (0.000) <(0.05), while the control group with a p-value (0.180)> (0.05). Conclusion: There is an effect of pregnancy exercise on third trimester primigravida anxiety in dealing with childbirth in the Wuryantoro Health Center work area. 

scholarly journals The Effect of Propanolol on C-Reactive Protein in Patients with Severe Burns at Dr Soetomo Hospital Surabaya

2021 ◽  
Vol 4 (1) ◽  
pp. 27
Author(s):  
Muhammad Aulia U H ◽  
Iswinarno Doso Saputro ◽  
Magda Rosalina Hutagalung
Keyword(s):  
Treatment Group ◽  
Albumin Level ◽  
Mean Value ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Severe Burns ◽  
Hypermetabolic State ◽  
Post Test ◽  
The Mean

Background: The incidence of burns in Indonesia progressively increases with the increase in its population and industries. From January to September 2000, 158 patients were treated in the burn unit of Dr Soetomo Hospital with a mortality rate reaching 5,8%. Burns have a direct effect in causing both local and systemic changes in the body, not occurring in other injuries. In severe burns, a hypermetabolic state can occur, which increases cardiac workload and causes muscle atrophy and other morbidities. The purpose of this study is to examine the effect of propranolol on the hypermetabolic state in severely burned patients by measuring various clinical & laboratory parameters.Methods : This is an experimental study using pre and post test control group design with the objective of assessing the treatment outcome with oral propranolol given in 15 consecutive days for burn patients involving 25%- 60% TBSA. Measurements were taken three times, on day 0, 7 and 14.Results : Obtained 16 samples divided into 2 groups. In the treatment group, there was a significant decrease in CRP levels on days 0, 7 and 14 (p <0.05). The Mid Arm Circumference variable did not obtain a significant decreasing on days 0, 7 and 14. The albumin level studied showed a significant decreasing on day 0 & 7 days with a value of p = 0.045. From the comparison between the two groups, there were significant differences in CRP levels on days 0 & 14, with the mean value of the treatment group -5.12 + 2.88 and the mean value of the control group 2.86 + 7.37, and the value of p = 0.019.Conclusions: This study successfully proved that the administration of propranolol can overcome the effects of hypermetabolism which is characterized by decreasing in CRP levels.

scholarly journals Perbedaan Kadar Glutation (GSH) Hepar Tikus Putih Jantan (Rattus norvegicus) yang diinduksi Parasetamol Dosis Toksik dengan Pemberian Ekstrak Daun Kelor (Moringa oleifera)

2020 ◽  
Vol 20 (1) ◽  
pp. 247
Author(s):  
Nur Insani ◽  
H.M.T Kamaluddin ◽  
Swanny Swanny
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Leaf Extract ◽  
The Body ◽  
Control Group ◽  
Bb Rat ◽  
Toxic Dose ◽  
Experimental Animals ◽  
Significant Difference ◽  
Group Ii

Glutathione (GSH) transferase deficiency due to paracetamol exposure causes further oxidative stress to liver necrosis. To reduce oxidative stress that can cause damage to the liver of the body requires antioxidants. One plant to treat liver disease is the kelor leaf (because it has an active flavonoid material also has antioxidant activity). This study was conducted to determine the difference of glutathione hepar levels of male white rat induced paracetamol toxic dose by giving kelor leaf extract. The type of research is experimental laboratory in vivo with rancagan randomized post test only control group design. With the stages as follows 1.Leaf Extract Kelor with Ethanol 96%, 2.Perpeteration of experimental animals, 3.Treatment of experimental animals by giving extract of 3-dose of kelor leaf that is KP I 250 mg / 200 gr BB rat, KP II 500 mg / 200 gr BB rat, KP III 1000 mg / 200 gr BB rat  for 14 days combined with paracetamol dose 2 gr / 200 gr BB rat compared with the negative control group (group given only paracetamol dose 2 gr / 200 gr BB rat) and control group positif only fed regular feed for 14 days). The result showed that there was a significant difference mean of GSH levels between all treatment groups obtained p = 0,000 (p <α) p values smaller than 0.05. There was the highest increase of GSH in treatment group II (142,7525 μmol / mg) and lowest dose of GSH in positive control group (57,1812 μmol / mg), dose paracetamol toxic dosage and kelor leaf extract 500 mg / gr BB rat can increase GSH hepar p = 0,000 (p <α) p less than 0 , 05. The conclusion of the test results showed that giving of kelor leaf extract at dose of treatment group II can increase GSH hepar level significantly

scholarly journals A Subpressor Dose of Angiotensin II Elevates Blood Pressure in a Normotensive Rat Model by Oxidative Stress

2015 ◽  
pp. 153-159 ◽  
Author(s):  
M. M. GOVENDER ◽  
A. NADAR
Keyword(s):  
Oxidative Stress ◽  
Blood Pressure ◽  
Angiotensin Ii ◽  
Mrna Expression ◽  
Rat Model ◽  
Wistar Rat ◽  
Control Group ◽  
Sod Activity ◽  
Male Wistar Rats ◽  
Experimental Group

Oxidative stress is an imbalance between free radicals and antioxidants, and is an important etiological factor in the development of hypertension. Recent experimental evidence suggests that subpressor doses of angiotensin II elevate oxidative stress and blood pressure. We aimed to investigate the oxidative stress related mechanism by which a subpressor dose of angiotensin II induces hypertension in a normotensive rat model. Normotensive male Wistar rats were infused with a subpressor dose of angiotensin II for 28 days. The control group was sham operated and infused with saline only. Plasma angiotensin II and H2O2 levels, whole-blood glutathione peroxidase, and AT-1a, Cu/Zn SOD, and p22phox mRNA expression in the aorta was assessed. Systolic and diastolic blood pressures were elevated in the experimental group. There was no change in angiotensin II levels, but a significant increase in AT-1a mRNA expression was found in the experimental group. mRNA expression of p22phox was increased significantly and Cu/Zn SOD decreased significantly in the experimental group. There was no significant change to the H2O2 and GPx levels. Angiotensin II manipulates the free radical-antioxidant balance in the vasculature by selectively increasing O2− production and decreasing SOD activity and causes an oxidative stress induced elevation in blood pressure in the Wistar rat.

scholarly journals EFFECT OF BACK EXERCISE TO DECREASE LOW BACK PAIN OF BATIK ARTISANTS IN KAUMAN VILLAGE, SOKARAJA

2019 ◽  
Vol 11 (1) ◽  
pp. 45
Author(s):  
Siti Harwanti ◽  
Budi Aji ◽  
Nur Ulfah
Keyword(s):  
Low Back Pain ◽  
Back Pain ◽  
Treatment Group ◽  
Exercise Therapy ◽  
The Body ◽  
Control Group ◽  
P Value ◽  
Low Back ◽  
Control Group Design ◽  
And Control

ABSTRACT Low back pain ( LBP ) is a musculoskeletal disorder that occurs in the lower back which can be caused by various diseases and  activity of the body being less good. One effort to overcome the LBP is back exercise. The research purpose is to find out the effect of back exercise therapy which concern to lowering painful complaint of batik artisants in Kauman village Sokaraja subsdistrict. This research is using Quasi Experiment technique phenomenological non equivalen control group design (pre -post test). Sample taking is using purposive sampling technique. Data analysis use Friedman experiment for treatment group and Repeated Annova experiment for control group. Experiment analysis is different between treatment group and control group with using Mann Whitney experiment. The result of Friedman experiment in treatment group shows that p-value=0,000(p<0,05), it means the therapy those given is effective to lowering painful complaint. The result of Repeated Annova experiment in control group shows that p-value=0,234(p>0,05), it means the painful complaint seems not different in control group. The result of Mann Whitney experiment shows that p-value=0,000(p<0,05) it means there are differences between painful complaint in treatment group and control group after doing back exercise. There is the effect by giving back exercise therapy which concern to lowering the complaint of low back pain for batik artisants in Kauman village Sokaraja subdistrict.   Key words: Handmade Batik, Low back pain, back exercise

scholarly journals Therapeutic response monitoring after targeted therapy in an orthotopic rat model of hepatocellular carcinoma using contrast-enhanced ultrasound: Focusing on inter-scanner, and inter-operator reproducibility

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244304
Author(s):  
Hwaseong Ryu ◽  
Jung Hoon Kim ◽  
Seunghyun Lee ◽  
Joon Koo Han
Keyword(s):  
Hepatocellular Carcinoma ◽  
Targeted Therapy ◽  
Treatment Group ◽  
Rat Model ◽  
Therapeutic Response ◽  
Response Monitoring ◽  
Control Group ◽  
Rat Models ◽  
Significant Change ◽  
Operator Reproducibility

Purpose To assess therapeutic response monitoring after targeted therapy in an orthotopic rat model of hepatocellular carcinoma (HCC) using CEUS with focusing on inter-scanner and inter-operator reproducibility. Materials and methods For reproducibility, CEUS was performed using two different US scanners by two operators in sixteen rat models of HCC. Using perfusion analysis software (VueBox ®), eleven parameters were collected, and intra-class correlation coefficient (ICC) was used to analyze reproducibility. Then seventeen rat models of HCC were divided into treatment group (n = 8, 30 mg/kg/day sorafenib for five days) and control group (n = 9). CEUS was performed at baseline and 14 days after first treatment, and changes of perfusion parameters were analyzed. Results In treatment group, CEUS perfusion parameters showed a significant change. The peak enhancement (PE, 2.50 x103±1.68 x103 vs 5.55x102±4.65x102, p = 0.010) and wash-in and wash out AUC (WiWoAUC, 1.07x105±6.48 x104 vs 2.65x104±2.25x104, p = 0.009) had significantly decreased two weeks after treatment. On the contrary, control group did not show a significant change, including PE (1.15 x103±7.53x102 vs 9.43x102± 7.81 x102, p = 0.632) and WiWoAUC (5.09 x104±3.25x104 vs 5.92 x104±3.20x104, p = 0.646). For reproducibility, the various degrees of inter-scanner reproducibility were from poor to good (ICC: <0.01–0.63). However, inter-operator reproducibility of important perfusion parameters, including WiAUC, WoAUC, and WiWoAUC, ranged from fair to excellent (ICC: 0.59–0.93) in a different scanner. Conclusion Our results suggest that CEUS is useful for assessment of the treatment response after targeted therapy and with fair to excellent inter-operator reproducibility.

scholarly journals Histologi Lumen Dan Endotelium Arteri Dorsal Penis Tikus Wistar (Rattus Novergicus) Yang Diinduksi Pakan Tinggi Lemak

2020 ◽  
Vol 7 (1) ◽  
pp. 73
Author(s):  
I Wayan Rosiana ◽  
I Gede Widhiantara
Keyword(s):  
Treatment Group ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Lumen Diameter ◽  
Control Group ◽  
High Fat ◽  
Arterial Lumen ◽  
Male Wistar Rats ◽  
The Difference

This study aims to look at the histopathological picture of the dorsal arteries of the penis of the hiperlipidemic wistar rats (Rattus novergicus) induction by high-fat diet that seen in terms of lumen diameter and thickness of the arterial endotelium wall. Hyperlipidemia is a risk factor for ateriosclerosis in the penile arteries causing erectile dysfunction in men. This study is an experimental study with a randomized posttest only control goup design. The sample are  10 individuals adult male wistar rats aged 3-4 months with a range of body weight 150-200 grams. Before treatment, adaptation was carried out for 7 days. After that the sample rats in the treatment group were made hyperlidemic by feeding lard for 50 days. Then surgery is performed for histopathological preparations at the posttest. To determine the differences in endotelium thickness and arterial lumen diameter in the two groups, an independent t-test was used. Thick diameter data of the endotelium and dorsal arteries of the penis of the wistar rat between the lower treatment group and the control group. The difference that occurred was statistically significant (p <0.05). So it can be concluded that the provision of high-fat diet (hyperlipidemia) decreases the lumen diameter and endotelium thickness of dorsal arteries penis. Keywords: Dorsal arteries, high-fat diet, Wistar rats

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