scholarly journals PROBIOTIC CANDIDATE PROTEOLYTIC Bacillus sp. COLLECTED FROM MANGROVE OF MARGASARI, LAMPUNG

2021 ◽  
Vol 8 (1) ◽  
pp. 73-82
Author(s):  
Sumardi Sumardi ◽  
Komang Rima ◽  
Salman Farisi ◽  
Endang Linirin Widiastuti

Intensive shrimp culture has encountered many problems, such as declining water quality through disease caused by pathogenic microbes, which affected mortality. This study aimed to determine any potential probiotic from Bacillus sp. collected from mangrove in East Lampung, which could be used to improve the cultured shrimps' proteolytic and probiotic activity. This is a descriptive research with sampling and data collection of bacteria from many samples of mangrove. Result shows 128 isolates Bacillus from which then it has arrived at five potential probiotic Bacillus sp. The study five Bacillus sp. has been isolated with potential properties for probiotic (KPP212, IP121, UJ131, UJ132, SB141). Each isolate has characteristics with proteolytic property, growth in a wide range of pH 4–10 and osmotic stress (0–6% NaCl), non-pathogenic, ability for glucose fermentation, non-motile, and has negative catalase activity. The five potential Bacillus sp. can be used as probiotics for shrimp farming.

2012 ◽  
Vol 3 (1) ◽  
pp. 30-33
Author(s):  
Thien Ngon Dang

Limited water exchange shrimp culture technology is commonly used today in many shrimp farms in Vietnam to reduce water usage, input of diseases and discharge of nutrient-rich effluents into environment as well as to increase the production per unit area. However, a remaining problem in this technology is that the water quality in shrimp ponds will be reduced due to limitation of water exchange for a long period. The accumulation of inorganic components such as waste feed, bacterial deposits or other biological debris at the pond bottom will lead to low dissolved oxygen, high ammonia-nitrogen level, high fecal coliform bacteria and high turbidity which cause a severe degradation of water quality and detriment to shrimp growth and survival. To solve this remaining problem, an autonomous water-cleaning machine for shrimp ponds was designed to control the waste accumulation in the pond. This is an effective solution to replace manual cleaning methods for water quality management in shrimp farming in the coastal area of the Mekong delta of Vietnam. Especially, this technique can be used for biosecure shrimp production systems according to GMP standards to meet the objectives for sustainable development of shrimp aquaculture in Vietnam. Kỹ thuật nuôi tôm không thay nước đang được sử dụng rộng rãi ở các trại nuôi tôm Việt Nam vì giúp giảm lượng nước sử dụng, hạn chế thải nước vào môi trường và giúp tăng diện tích nuôi trồng tôm. Tuy nhiên, bản thân kỹ thuật này cũng tạo nên một sản phẩm chất thải là phân tôm, thức ăn và chế phẩm sinh học xử lý nước dư thừa. Chất thải này dần dần tích tụ dưới đáy ao tạo thành lớp bùn độc, rất thiếu ôxy và chứa nhiều chất gây hại như ammonia, nitrite, hydrogen sul-fide. Để tránh làm giảm diện tích ao nuôi do chất thải tích tụ làm tôm lảng tránh và tăng mật độ tôm nuôi trồng, thiết bị tự hành thu gom chất thải làm sạch nước trong vuông nuôi tôm đã được nghiên cứu, thiết kế và chế tạo thành công. Thiết bị đã thay thế các hoạt động làm sạch chất thải thủ công của con người, không sử dụng các nguồn năng lượng gây ô nhiễm, giảm nhu cầu về điện góp phần phát triển sản xuất tôm sạch đạt chuẩn GMP và phát triển bền vững ở các tỉnh ven biển miền Tây Việt Nam.


2021 ◽  
Vol 12 (1) ◽  
pp. 18-28
Author(s):  
Heri Ariadi ◽  
Abdul Wafi ◽  
Muhammad Musa ◽  
Supriatna Supriatna

Water quality parameters play an important role in intensive pond ecosystems. The purpose of this study was to determine the relationship between of water quality parameters in intensive shrimp farming of L. vannamei. This research was carried out for 95 days of intensive shrimp farming in PT. Menjangan Mas Nusantara Company, Banten, with the physical, chemical, and microbiological parameters of water as the main reference object of observation. The results showed that during the shrimp culture period the pond water quality parameter concentration was considered to be quite optimal with a stable fluctuation trend, except for the salinity and TOM parameters whose values ​​were above the water quality standard. Correlation test results state that between the physical chemical parameters have a strong and heterogeneous relationship, with the strongest parameters of pH, phosphate, nitrite, and TOM. As for the microbiological variables, the correlation of physical chemistry parameters of water is considered to be very weak, because from the correlation test results, only DO parameters showed the correlation with microbiological parameters. The conclusion of this study, that during intensive shrimp culture period, the physical and chemical parameters of water have a strong correlation of association between one another and the highest are pH, phosphate, nitrite, and TOM, but only dissolved oxygen parameters that show the relationship correlation with microbiological parameters.


DEPIK ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 217-226
Author(s):  
Supono Supono

Abstract. Biofloc technology in shrimp farming is a new technology that has advantages over other technologies. Biofloc technology can maintain water quality, especially ammonia and pH, suppress Vibrio growth, enhance immunity, and as natural food for shrimp. The application of this technology in shrimp farming still faces several obstacles, so not all farmers apply it. This study aims to evaluate the application of biofloc systems in white shrimp farming and formulate appropriate strategies to increase farmer productivity. The study was conducted using the survey and interview method for the perpetrators of white shrimp cultivation in Lampung Province. Collected data were strengths, weaknesses, opportunities, and threats of the application of biofloc technology in the cultivation of white shrimp. The obtained data were analyzed descriptively, while the formulation of strategies was conducted using SWOT analysis. The results showed that shrimp culture technology with biofloc technology has good prospects for increasing shrimp production. However, biofloc technology also has weaknesses that can lead to the failure of shrimp farming. The application of white shrimp biofloc technology was in quadrant I (aggressive) where this system has a good internal strength to capture existing external opportunities (SO strategy). Internal strengths include a high survival rate, relatively faster growth, environmentally friendly, able to minimize disease agents that enter the culture system, and lower feed conversion, while the opportunities they have include: shrimp prices are relatively high, there is a revitalization program unproductive ponds by the government, the issue of eco-labeling, and the growing demand for shrimp exports.Keywords: SWOT analysis, water quality, strategy, shrimp production, aggressive  Abstrak. Teknologi biofloc dalam budidaya udang merupakan teknologi baru yang memiliki keunggulan dibandingkan teknologi lainnya.  Teknologi biofloc dapat menjaga kualitas air terutama amoniak dan pH, menekan pertumbuhan Vibrio, meningkatkan imunitas, serta sebagai pakan alami bagi udang.  Penerapan teknologi ini dalam budidaya udang masih mengalami beberapa kendala, sehingga tidak semua petambak menerapkannya.  Penelitian ini bertujuan untuk mengevaluasi penerapan sistem biofloc  dalam budidaya udang putih dan merumuskan strategi yang tepat untuk meningkatkan produktivitas petambak. Penelitian dilakukan dengan metode surve dan wawancara terhadap pelaku budidaya udang  putih di Provinsi Lampung.  Data yang dikumpulkan berupa kekuatan (strength), kelemahan (weakness), peluang (opportunity) dan ancaman (threat) penerapan teknologi biofloc dalam budidaya udang putih.  Data yang diperoleh dianalisis secara deskriptif, sedangkan perumusan strategi menggunakan   analsis SWOT.  Hasil penelitian menunjukkan bahwa teknologi budidaya udang dengan teknologi biofloc mempunyai prospek yang baik untuk meningkatkan produksi udang.  Namun demikian,  teknologi biofloc juga memiliki kelemahan yang dapat menimbulkan kegagalan budidaya udang. Penerapan teknologi  biofloc udang putih berada pada kuadran I (agresif) dimana sistem ini mempunyai kekuatan internal yang baik untuk menangkap peluang eksternal yang ada (strategi SO).  Kekuatan internal yang dimiliki antara lain: tingkat kelulushidupan tinggi, pertumbuhan relatif lebih cepat, ramah lingkungan, mampu meminimalisir agen penyakit yang masuk dalam sistem budidaya, dan konversi pakan lebih rendah, sementara peluang yang dimiliki antara lain: harga udang relatif tinggi, adanya program revitalisasi tambak yang tidak produktif oleh pemerintah, adanya isu eco labeling, serta permintaan ekspor udang yang terus meningkat.Kata kunci: Analisis SWOT, kualitas air, strategi, produksi udang, agresif


2018 ◽  
Vol 54 (1) ◽  
pp. 34-46
Author(s):  
Navid Dolatabadi Farahani ◽  
Hamid Taheri Shahraiyni ◽  
Reza Sheikhi

Abstract In this study, the water quality of the Bahmanshir River and its water channels where Choebdeh Shrimp Farms (the largest shrimp culture complex in Iran) are located were simulated using MIKE11 software. First, an integrated hydraulic and salinity model of the river and its water channels was developed. Then, Manning and dispersion coefficients of the river were calibrated and validated. The most important parameters in the water quality model were determined by sensitivity analysis and these parameters were calibrated using in situ measured water quality data. The errors of salinity, temperature, nitrate, ammonia and dissolved oxygen (DO) models in the verification step were 7.9, 1.2, 0.34, 0.79 and 12%, respectively. Then, two scenarios were applied to the river and the effects of these scenarios on the water quality of the river and its channels were evaluated. The results demonstrated that the site selection of the shrimp culture complex had been performed well because different scenarios could not affect the water quality in the channels. Finally, the water quality in the channels was compared with the standard values of shrimp survival parameters. All of the parameters in the channels were in the range of standard values except DO, which was slightly under the standard value.


Author(s):  
Le The Truyen ◽  
Le Thanh Long

The development of new technologies in automation to increase labor productivity has been increasingly enhanced in recent decades. The problem of cleaning water in shrimp ponds greatly affects the quality as well as shrimp production. Environmental pollution of shrimp farming is a matter of concern because the current waste treatment solutions are not yet thorough. A waste remover of shrimp waste combined with the pond bottom siphon method has been researched and developed to increase the ability to thoroughly handle waste generated in the culture environment. This device helps to automate the manual cleaning of the pond bottom by farmers. The device performs operations to clean waste, suck, filter, and remove waste from the culture environment. This device is self-propelled or manually controlled and operates in all weather conditions. This article introduces the process of testing and evaluating the efficiency of waste extraction equipment in shrimp ponds. The device was tested at a super-intensive shrimp farm and evaluated for operational efficiency. The experimental model consists of a shrimp pond operating a waste suction device, a control pond, an automatic monitoring system of water quality parameters (DO, H2S, NH3, pH, and temperature). Experimental ponds operating waste disposal equipment, control ponds are manually cleaned, other farming conditions of the two ponds are similar. The impacts of waste on the shrimp culture environment are determined through analyzing the results of measuring water quality criteria in the pond, thereby assessing the efficiency of waste removal of the equipment. The measurement results show that water quality parameters reach a value within the threshold if operating a waste suction device once per day. The benefits of waste remover operate to help save the cost of labor to clean the pond bottom, protect workers' health.


2021 ◽  
Vol 4 ◽  
Author(s):  
Luiz Drude de Lacerda ◽  
Raymond D. Ward ◽  
Mario Duarte Pinto Godoy ◽  
Antônio Jeovah de Andrade Meireles ◽  
Rebecca Borges ◽  
...  

Brazilian mangroves cover about 11,100 km2 and provide a wide range of ecosystem services. Despite their importance, they are one of the most impacted ecosystems because of combined influences of climate change, pollution, and direct conversion and loss. A major driver of environmental impacts is shrimp farming and this is particularly acute in the semi-arid northeast of Brazil, where mangroves are constrained in a narrow band along ephemeral estuaries that are often impacted by multi-year droughts. Recent changes to Brazilian law, in particular the Forest Code, have weakened protection for mangroves and associated “apicum” (salt pan) ecosystems. In NE Brazil, most shrimp ponds are converted from mangrove-adjacent “apicuns” rather than the mangroves themselves with periodic hydrological connectivity through dammed channels, allowing the flushing of effluents. As a result, the main impacts on mangroves are typically indirect, because of pollution inputs from shrimp pond effluents and associated loss of ecosystem services including reductions in primary productivity, carbon storage capacity, resilience to other environmental stressors, their efficiency as estuarine filters, and biodiversity and abundance of subsistence use of marine species. Soil damage and infrastructure remaining after shrimp pond deactivation impairs mangrove recovery. This extends the duration of the damage and allows the occupation of degraded areas by other activities that can permanently impair ecosystem function. In this review, we address several aspects of the shrimp culture boom in NE Brazilian, their features and consequences, and the future of mangroves in the region considering climate change and rising poverty. Our conclusions on the practices and outcomes of shrimp farming in mangroves are likely to apply to areas with similar environmental settings, e.g., semiarid regions worldwide, and particularly in the Latin America and Caribbean region, and our findings can be taken into account to improve conservation and management of these forests at the least to a regional scale.


Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1443
Author(s):  
Yoshiaki Kamiyama ◽  
Sotaro Katagiri ◽  
Taishi Umezawa

Reversible phosphorylation is a major mechanism for regulating protein function and controls a wide range of cellular functions including responses to external stimuli. The plant-specific SNF1-related protein kinase 2s (SnRK2s) function as central regulators of plant growth and development, as well as tolerance to multiple abiotic stresses. Although the activity of SnRK2s is tightly regulated in a phytohormone abscisic acid (ABA)-dependent manner, recent investigations have revealed that SnRK2s can be activated by group B Raf-like protein kinases independently of ABA. Furthermore, evidence is accumulating that SnRK2s modulate plant growth through regulation of target of rapamycin (TOR) signaling. Here, we summarize recent advances in knowledge of how SnRK2s mediate plant growth and osmotic stress signaling and discuss future challenges in this research field.


Author(s):  
Cristián Raziel Delgado-González ◽  
Alfredo Madariaga-Navarrete ◽  
José Miguel Fernández-Cortés ◽  
Margarita Islas-Pelcastre ◽  
Goldie Oza ◽  
...  

Potable and good-quality drinking water availability is a serious global concern, since several pollution sources significantly contribute to low water quality. Amongst these pollution sources, several are releasing an array of hazardous agents into various environmental and water matrices. Unfortunately, there are not very many ecologically friendly systems available to treat the contaminated environment exclusively. Consequently, heavy metal water contamination leads to many diseases in humans, such as cardiopulmonary diseases and cytotoxicity, among others. To solve this problem, there are a plethora of emerging technologies that play an important role in defining treatment strategies. Phytoremediation, the usage of plants to remove contaminants, is a technology that has been widely used to remediate pollution in soils, with particular reference to toxic elements. Thus, hydroponic systems coupled with bioremediation for the removal of water contaminants have shown great relevance. In this review, we addressed several studies that support the development of phytoremediation systems in water. We cover the importance of applied science and environmental engineering to generate sustainable strategies to improve water quality. In this context, the phytoremediation capabilities of different plant species and possible obstacles that phytoremediation systems may encounter are discussed with suitable examples by comparing different mechanistic processes. According to the presented data, there are a wide range of plant species with water phytoremediation potential that need to be studied from a multidisciplinary perspective to make water phytoremediation a viable method.


Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 40
Author(s):  
Liang Cui ◽  
Bitong Zhu ◽  
Xiaobo Zhang ◽  
Zhuhua Chan ◽  
Chungui Zhao ◽  
...  

The elevated NH3-N and NO2-N pollution problems in mariculture have raised concerns because they pose threats to animal health and coastal and offshore environments. Supplement of Marichromatium gracile YL28 (YL28) into polluted shrimp rearing water and sediment significantly decreased ammonia and nitrite concentrations, showing that YL28 functioned as a novel safe marine probiotic in the shrimp culture industry. The diversity of aquatic bacteria in the shrimp mariculture ecosystems was studied by sequencing the V4 region of 16S rRNA genes, with respect to additions of YL28 at the low and high concentrations. It was revealed by 16S rRNA sequencing analysis that Proteobacteria, Planctomycete and Bacteroidetes dominated the community (>80% of operational taxonomic units (OTUs)). Up to 41.6% of the predominant bacterial members were placed in the classes Gammaproteobacteria (14%), Deltaproteobacteria (14%), Planctomycetacia (8%) and Alphaproteobacteria (5.6%) while 40% of OTUs belonged to unclassified ones or others, indicating that the considerable bacterial populations were novel in our shrimp mariculture. Bacterial communities were similar between YL28 supplements and control groups (without addition of YL28) revealed by the β-diversity using PCoA, demonstrating that the additions of YL28 did not disturb the microbiota in shrimp mariculture ecosystems. Instead, the addition of YL28 increased the relative abundance of ammonia-oxidizing and denitrifying bacteria. The quantitative PCR analysis further showed that key genes including nifH and amoA involved in nitrification and nitrate or nitrite reduction significantly increased with YL28 supplementation (p < 0.05). The supplement of YL28 decreased the relative abundance of potential pathogen Vibrio. Together, our studies showed that supplement of YL28 improved the water quality by increasing the relative abundance of ammonia-oxidizing and denitrifying bacteria while the microbial community structure persisted in shrimp mariculture ecosystems.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 89-89
Author(s):  
Brigit Lozinski ◽  
Brent Frederick ◽  
Adrienne Hilbrands ◽  
Yuzhi Li ◽  
Milena Saqui-Salces ◽  
...  

Abstract Newly-weaned pigs (n = 450; age = 20 d) were used in a 40-d experiment to determine the effects of water quality on pig performance and health. Pigs were sourced from a single commercial sow farm that was negative for both Porcine Reproductive and Respiratory Syndrome virus and Mycoplasma Pneumonia. Pigs were allotted randomly to 45 pens (10 pigs/pen) and pens were assigned to 1 of 3 water treatments that provided water of differing quality. Waters were selected to represent a wide range of perceived water quality. Water quality was determined based on concentration of analytes including sulfates (1,120; 617; 2 ppm), iron (5.4; 5.2; 1.3 ppm), Total Dissolved Solids (TDS; 1,500; 1,050; 348 ppm), hardness (1,410; 909; 235 mg Eq CaCO3/L), magnesium (171; 91; 21 ppm) and sodium (64; 37; 29 ppm) for waters A, B, and C, respectively. Pigs were housed in an environmentally controlled, power ventilated, confinement nursery barn and were allowed ad libitum access to a common three-phase diet and water across all water treatments. Weekly ADG, ADFI, and G:F were measured and subjective scour score (1=solid feces to 4=liquid feces) was recorded daily through day 7. Data for pig growth performance were analyzed using PROC GLIMMIX with water quality as a fixed effect and pen served as the experimental unit. There were no differences among treatments in ADG, ADFI, and G:F (Table 1). Number of pigs treated with antibiotics throughout the experiment did not differ among treatments as determined by Chi-Square analysis. Average subjective scour score on day 7 of the experiment also did not differ among treatments. In conclusion, performance and health of nursery pigs as measured in this experiment were not influenced by the differing water qualities studied.


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