scholarly journals PENGARUH EKSTRAK ETANOL DAUN JALOH (Salix Tetrasperma Roxb) TERHADAP PERSENTASE PARASITEMIA PADA MENCIT (Mus musculus) YANG DIINFEKSI Plasmodium berghei

Author(s):  
Nuzul Asmilia ◽  
Sugito Sugito ◽  
Erdiansyah Rahmi ◽  
Niko Febrianto

This study aimed to verify the effect of ethanol extract of jaloh leaves at various doses on parasitemia percentage of in mice (Mus musculus), wich inoculated Plasmodium berghei. This study used 12 male mice (Mus musculus) strains Balb / C with average body weight of 30.2 grams of 2-month-old. This research using Completely Randomized Design (CRD) with four treatments and three replications. All the mice were infected with Plasmodium berghei prior to treatment on three days post infected the mice in group 2,3 and 4 were given with extract ethanol jaloh leaves at dose 50, 100, 150 mg/kgbw inspectively. The mice in group 1 (K0) were not given anything and consident as control.Observations parasitemia percentage using microscopy on preparations stained thin blood smear Giemsa. The parasitemia percentage data were analyzed using one way ANOVA. On the average (±SD) percentage of parasitemia during the three days in four treatments (K0, K1, K2, and K3) on the first day were 24.87±1.86; 24.33±1.53; 24.20±1.06; and 24.93±1.01. On the second day were 22.67±2.52; 23.00±1.00; 22.80±1.20; and 24.13±2.01; and on the third day were 24.77±1.66; 22.97±3.76; 21.13±1.90; and 24.27±1.42. Statistical analysis showed that ethanol extract of the jaloh leaves for KO, KI, KII, and KIV respectively treatment did not affect the percentage of parasitemia in mice (Mus musculus) inoculated Plasmodium berghei.

2012 ◽  
Vol 13 (4) ◽  
pp. 1099-1111 ◽  
Author(s):  
Emiliano Nunes do Nascimento ◽  
Marcos Cláudio Pinheiro Rogério ◽  
Ana Sancha Malveira Batista ◽  
Maria Socorro de Souza Carneiro ◽  
Angela Maria de Vasconcelos ◽  
...  

This work aimed to determine the nutrient intake and quantitative aspects of sheep carcass from three different genetic groups. Eighteen animals with initial average body weight of 20.1kg were used in a completely randomized design, in a factorial 3x2 scheme (three different crossbreeds - 6 F1Dorper x non defined breed, 6 F1Somalis x non defined breed and 6 F1Santa Inês x non defined breed versus two diets). The diets fed to the animals were formulated with or without cashew nuts. The inclusion of cashew nut meal in the diet influenced nutrient intake. Differences of intake among the animal groups were observed as higher intake for F1Dorper x non defined breed (NDB), followed by F1Somalis x NDB and for F1Santa Inês x NDB. However, the control diet produced no difference among these genotypes. The average daily gain was lower for both crossbreeds when cashew nut meal was added in the diets. The evaluated crossbreeds and diets did not affect the feed:gain ratio. The carcasses analyses showed that the inclusion of cashew nut meal in the diets reduced the cold carcass weight and the rib weight. The addition of cashew nut meal in the diets for lambs reduces food intake and carcass yield. The crossbreed F1Dorper x NDB presented the higher rate of average daily gain.


2019 ◽  
Vol 40 (6) ◽  
pp. 2795
Author(s):  
José Assunção Silveira Junior ◽  
Márcio dos Santos Pedreira ◽  
Christian Albert Carvalho da Cruz ◽  
Cristiane Leal dos Santos-Cruz ◽  
Cláudio Eduardo Silva Freitas ◽  
...  

The objective of this study was to evaluate carcass yield, measurements, cuts, and fat deposition of sheep fed banana pseudostem hay and Tifton 85 grass hay, with or without virginiamycin. Thirty-three uncastrated male Dorper × Santa Ines crossbred lambs at five months of age, with an average body weight of 25.00 ± 1.95 kg, were used in a feedlot experiment. The following four diets were evaluated: Tifton 85 grass hay plus concentrate with virginiamycin, banana pseudostem hay replacing 60% of Tifton 85 grass hay plus concentrate with virginiamycin, Tifton 85 grass hay plus concentrate without virginiamycin, and banana pseudostem hay replacing 60% of Tifton 85 grass hay plus concentrate without virginiamycin. The experiment was set in a completely randomized design, in a factorial scheme, using orthogonal contrasts. The diet replacing 60% Tifton 85 grass hay by banana pseudostem hay without virginiamycin had no effect on carcass traits. The combination of virginiamycin and Tifton 85 grass hay provided increases in warm carcass weight, warm carcass yield, spine, omental and mesenteric fat deposition, and, regardless of roughage, increases in the thoracic perimeter.


2016 ◽  
Vol 37 (5) ◽  
pp. 3349
Author(s):  
Ana Clara Bohnen de Barros ◽  
João Restle ◽  
Regis Luis Missio ◽  
Fabrícia Rocha Chaves Miotto ◽  
Wescley Faccini Augusto ◽  
...  

Assessment of carcass and meat characteristics of Holstein-Zebu crossbred young bulls finished in feedlot with crude glycerin levels (0, 60, 120, and 240 g kg-1 dry matter) in replace of ground pearl millet grain and babassu mesocarp bran mixture-based diets. Twenty four bulls 30-month-old and initial average body weight of 390 ± 31.5 kg were used. The animals were distributed in a completely randomized design with four treatments and six replicates. The absolute weight (kg) of commercial cuts did not fit any of the tested regression models. However, the weight of the short ribs and the pistol cut were higher for animals fed with crude glycerin in relation to those fed without inclusion of this food in the diet. The proportion of the commercial cuts, however, was not changed. The increase of the crude glycerin levels linearly increased the proportion of carcass fat and reduced the muscle proportion, without changing the proportion of bone and the edible portion of the carcasses. The ratio muscle/fat reduced linearly with the advance of crude glycerin level of diets. The color of the meat, evaluated subjectively, increased linearly with the advance of crude glycerin levels of the diets, from dark red to slightly dark red. The texture, marbling, shear force, thawing loss and cooking loss were not changed by dietary levels of crude glycerin. The inclusion of up to 240 g kg-1 of crude glycerin in the diets increases the proportion of fat without changing the marketable portion of carcasses. The benefits to the meat quality refer to the improvement of color, without changing the other characteristics of economic interest.


ZOOTEC ◽  
2019 ◽  
Vol 39 (2) ◽  
pp. 345
Author(s):  
Julin Salauhiang ◽  
Marie Najoan ◽  
Cherly J. Pontoh ◽  
Meity R. Imbar

EFFECT OF REPLACEMENT SOME RATIONS WITH FLOUR YELLOW PUMPKIN WASTE (CUCURBITA MOSCHATA) AGAINST PERFORMANCE BROILER. This study aims to determine   the extent of the partial replacement of rations with pumpkin waste flour (Cucurbita moschata) as measured by broiler performance. Using 100 unsexed broiler Cobb 707 aged 1 day with average body weight ± 45.32 g. The design used was a Completely Randomized Design (CRD) consisting of 4 treatments and 5 replications. The treatment composition is as follows: R0 = 100% basal ration (RB) + 0% pumpkin waste flour (TLLK), R1 = 95% RB + 5% TLLK, R2 = 90% RB + 10% TLLK, R3 = 85% RB + 15% TLLK.  Results of analysis of variance showed that the dissecting treatment was not significant (P>0.05) for the consumption, weight and conversion of ration The use of pumpkin waste flour can replace a portion of the ration up to 15%.Key words: Performance, waste pumpkin, broiler.


2021 ◽  
Vol 7 (1) ◽  
pp. 74-84
Author(s):  
I Komang Alit Saputra ◽  
Ni Gusti Ayu Manik Ermayanti ◽  
A.A. S. A. Sukmaningsih

This study aims to determine the effect of given robusta coffee (Coffea canephora Pierre ex A. Froehner) leaf extract on the spermatozoa quality of mice (Mus musculus L.) exposed to cigarette smoke. The experiment was using a completely randomized design (CRD) with five treatments and each consisted of six mice as replications. Each treatment was K- (without exposure to cigarette smoke), K+ (exposure to cigarette smoke + 0.5% Na-CMC), P1 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 60 mg/kgbw), P2 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 120 mg/kgbw) and P3 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 180 mg/kgbw). On the 36th day, mice were taken down by anesthesia and followed by surgery to collect spermatozoa. The parameters for this observation were the concentration, motility, viability, morphology, and integrity of the spermatozoa membrane. The data obtained were analyzed statistically with One Way Anova and further tested with DMRT. The results showed that the treatment of robusta coffee leaf exstract in mice exposed to cigarette smoke had a significant effect (P<0,05) on the quality of spermatozoa.


Author(s):  
Kusmardi Kusmardi ◽  
Berna Elya ◽  
Tri Wahyuni ◽  
Rafika Indah Paramita

Objective: Anemia in acute malaria occurs because there is massive lysis of erythrocytes as a result of an increase in parasitemia or use of drugs that may induce hemolysis. In this research, it was tested the effect of administration of a combination of sambiloto (Andrographis paniculata Nees) extract and spirulina (Arthrospira platensis Gomont). This combination is aimed to obtain antiparasite activity of sambiloto and increasing of hemoglobin (Hb) from spirulina.Methods: This research was conducted with a completely randomized design using 75 mice of Swiss Webster strain. Test groups were consisted of AP, AP+ES, AP+PS, carboxymethyl cellulose, and dihydroartemisinin-piperaquine. All mice were infected with Plasmodium berghei on the day 0. The test material extracts were given 3 days before infection (H-3) and every day for 28 days after infection. Parasitemia data were taken on day 3, 7, 10, 15, 21, and 28, while the data for erythrocyte count and Hb level were taken on day 3, 10, and 21. Data processing was conducted using one-way ANOVA, followed by post hoc test.Results: This study showed that combination of sambiloto extract and spirulina powder (AP+PS) gave a significant difference result in suppressing the percentage of parasitemia (p=0.02), increasing the erythrocyte count (p=0.03), and Hb level (p=0.01) at the 15th day, compared to the group given only sambiloto (AP).Conclusion: Combination of spirulina powder and sambiloto extract will decrease parasitemia and increase the number of erythrocyte and Hb in mice that infected by P. berghei.  


2018 ◽  
Vol 6 (1) ◽  
pp. 97
Author(s):  
Budi Ayuningsih ◽  
Iman Hernaman ◽  
Diky Ramdani ◽  
Siswoyo Siswoyo

This research aimed to study the effect of protein and energy on Diet Use Efficiency of female Garut sheep. Four different treatments diets of different protein to TDN (energy) ration such as R1=11.53%:63.13%; R2=10.78%:70.06%; R3=13.54%:63.18%; and R4=13.67%:69.18% were allocated to 20 heads of female Garut sheep with 7-8 months old and average body weight 15.72 ± 1.33 kg. Completely randomized design was used and collected data  was analyzed with Duncan’s test. The results showed that highest diet use efficiency and protein consumption (P<0.05) were seen in R4 treatment, 13.09% and 94.04 g/day. Meanwhile, TDN consumption did not have any different among R4 (480.23 g/day), R2 (474.80 g/day), and R1 (446.77 g/day) but they were higher (P<0.05) than R3 (370.87 g/day). In other hand, TDN digestibility of R2 and R3 were higher than R1 and R3. In conclusion, protein to TDN ratio of 13.67%:69.18% resulted highest diet use efficiency. Keywords: Female Garut Sheep, Digestibility, Protein, Total Digestible Nutrient 


ZOOTEC ◽  
2020 ◽  
Vol 40 (1) ◽  
pp. 316
Author(s):  
Alden S. Bawole ◽  
Florencia N. Sompie ◽  
Jein R. Leke ◽  
Youdhie H.S. Kowel

THE EFFECT OF BASAL RATIONS REPLACEMENT WITH COCONUT OIL IN  RATION ON THE PERFORMANCE OF SUPER NATIVE CHICKEN GROWER PHASE. This study aims to find out the extent of replacement of some basal rations with coconut oil towards the performance of grower phase super native chickens. The material used was 100 super native chicken aged 8 weeks with an average body weight of ± 866.55g. The design used was a Completely Randomized Design (CRD) which consisted of five treatments and five replications. The structure of the treatment was as follows: R0 = 100% of basal rations (RB) + 0% of coconut oil, R1 = 99.5% of RB + 0.5% of coconut oil, R2 = 99% of RB + 1% of coconut oil, R3 = 98,5% of RB + 1.5% coconut oil, R4 = 98% of RB + 2% of coconut oil. The results of research showed that the treatment was very significantly different (P<0.01) to the consumption. Based on the results of the study, it can be concluded that the use of coconut oil up to the level of 2% gave good results on the performance of super native chickens. Keywords: Performance, Coconut Oil, Super native Chicken.


2020 ◽  
Vol 3 (1) ◽  
pp. 7-12
Author(s):  
Okta Malia ◽  
Dian Samitra ◽  
Eka Lokaria

This study aims to determine the effect of the water boiled leaves of Clidemia hirta on blood sugar levels in Swiss Webster mice (Mus musculus). This research using a completely randomized design research design (CRD) with 5 treatment groups namely; Aquades control group (P0), 3 g sucrose treatment (P1), 3 g sucrose treatment + boiled bristle leaves boiled water at a dose of 0.13 ml/KgBB (P2), 3 g sucrose treatment + boiled bristle leaves boiled water at a dose of 0.26 ml/KgBB (P3), 3 g treatment of sucrose + boiled bristle leaves boiled water at a dose of 0.39 ml/KgBB (P4). Before administration of the extract, mice in the treatment groups P1, P2, P3 & P4 were induced using 3 g of sucrose for 5 days. Then after 5 days for groups P2, P3, and P4 followed by giving boiled water leaves for 7 days. The analysis result of One Way Anova Test shows that Fcount > Ftable (18.86 > 2.51). This shows the boiled water affects the blood sugar levels of male Swiss Webster mice. The concentration of extract at P4 (0.39 ml/KgBB) was more effective in reducing blood sugar levels.


2002 ◽  
Vol 3 (1) ◽  
pp. 1-5
Author(s):  
Amir Hasan Lubis

ABSTRACT. Studies on response of broiler to utilization of cassava meal as substitute for cron diet were conducted using seventy two unsexed hubbard strain broiler chiks. A completely randomized design, with four treatment and six replicates was used and this study. Four treatment consisting of cassava meal were supplemented in each ration at graded level of 0%, 10 %, 20%, and 30%. Each treatment contains 22.5% protein and 3000 cal/kg ration metabolized energy. The result showed there were no significant effects of the four level of cassava meal on body weight, feed consumption, feed conservasion, percentage of abdominal fat. The average body weight for each treatment was 1508.91,1454.99,1417.31 and 1401.95 gram, respectively. Feed conservation was 1.72, 1.72, 1.72 and 1.74 gram, respectively. Percentage of abdominal fat was 2.64, 2.87, 3.11 and 3.14%, respectively. There was a tendency that the increasing level of cassava meal in ration will decresease body weight and feed consumtion. It could be concluded that utilization of cassava meal up to 30% to substitutebcorn in the ration might recommended.


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