Soybean Oligosaccharides Supplementation Attenuates the Fecal Odor Compounds by Modulate the Cecal Microbiota of Broilers

Abstract Background: Abatement of odor emissions in poultry production is very important for the quality and safety in poultry industries and benefit to the environment.Methods: This study was conducted to evaluate the effects of the dietary supplementation of different levels soybean oligosaccharides (SBO) in comparison with chlortetracycline (CHL) on major odor-causing compound in excreta and cecal microbiota of broiler chickens. One-day-old broiler chickens were assigned to 6 treatments with 6 replicate pens (10 birds/pen) for the 42-day experiment, including, the negative control (NC) fed a basal diet, the positive control (PC) fed a basal diet with CHL, and the basal diet with SBO at 0.5, 2.0, 3.5, and 5.0 g/kg, respectively. Fresh excreta was sampled for analysis odor compounds by high performance liquid chromatography. Cecum content was collected to analyze the cecal microbiota by 16S rRNA sequencing. Results: The excreta indole concentration of broilers fed 2.0, 3.5 and 5.0 g/kg SBO and CHL diets were significantly decreased (P < 0.01) compared to NC. Excreta skatole concentration (P < 0.001) and pH (P < 0.05) were decreased by SBO and CHL. Formate concentration of birds fed 3.5 and 5.0 g/kg SBO diets were higher than that of birds fed other diets (P < 0.001). The acetate concentration (P = 0.003) were increased in birds fed 3.5 g/kg SBO diet. Deep sequencing 16S rRNA revealed that the composition of the cecal microbial digesta slightly or significantly changed by the supplementation of SBO or CHL. SBO decreased the abundance of Bacteroides, Bilophila, and Escherichia, which were related to indole and skatole concentration of excreta. While CHL had strong tendency to enrich Ruminococcus and reduce Rikenella. Conclusion: These results indicated that supplementation of dietary SBO was beneficial in attenuating the concentration of odor causing compounds and impact the composition cecal microbiota of broilers.

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Growth Performance, Serum Biochemical Indices, Duodenal Histomorphology, and Cecal Microbiota of Broiler Chickens Fed on Diets Supplemented with Cinnamon Bark Powder at Prestarter and Starter Phases

Animals ◽

10.3390/ani11010094 ◽

2021 ◽

Vol 11 (1) ◽

pp. 94

Author(s):

Mohammed M. Qaid ◽

Saud I. Al-Mufarrej ◽

Mahmoud M. Azzam ◽

Maged A. Al-Garadi ◽

Hani H. Albaadani ◽

...

Keyword(s):

Body Weight ◽

Broiler Chickens ◽

Body Weight Gain ◽

Basal Diet ◽

Positive Control ◽

Negative Control ◽

Broiler Chicks ◽

Feed Conversion ◽

Serum Globulin ◽

Maximum Improvement

Ross 308 broiler chicks (n = 240) aged 1 day were assigned to five groups for eight replicates (six chicks for each) (3♂ and 3♀). Basal dietary groups were supplemented by 2000, 4000, and 6000 mg/kg cinnamon (CN) for 21 days. Basal diet alone was used as a negative control, and basal antibiotic diet (Colimox) was used as a positive control. At 10, 14, and 21 days of age, chicks that received 2000 mg CN and Colimox had a higher body weight, resulting in an increase in body weight gain. CN also resulted in the maximum improvement in the feed conversion ratio and feed efficiency over 1–21 days at the level of 2000 mg/kg. At days 10, the maximum relative breast weight was 2000 mg/kg of CN. Mean serum albumin concentrations, duodenal villus height, and goblet cell density increased (p < 0.05) by 2000 mg/kg of CN, and mean serum globulin and total protein concentrations and crypt depth increased (p < 0.05) by 6000 mg/kg of CN compared with control. Increased cecal Escherichia coli number was CN dose-dependent. In conclusion, dietary inclusion of 2000 mg/kg CN can be applied as an alternative to in-feed antibiotics for broiler starter diet.

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Dietary inulin supplementation modulates the composition and activities of carbohydrate-metabolizing organisms in the cecal microbiota of broiler chickens

PLoS ONE ◽

10.1371/journal.pone.0258663 ◽

2021 ◽

Vol 16 (10) ◽

pp. e0258663

Author(s):

Yun Xia ◽

Jiarong Miao ◽

Yu Zhang ◽

Hongbo Zhang ◽

Lingdong Kong ◽

...

Keyword(s):

Energy Metabolism ◽

16S Rrna ◽

Glycoside Hydrolase ◽

Broiler Chickens ◽

Basal Diet ◽

Attractive Alternative ◽

Optimal Dosage ◽

Growth Promoters ◽

Antibiotic Growth Promoters ◽

Cecal Microbiota

Inulin is a highly effective prebiotic and an attractive alternative to antibiotic growth promoters for increasing production and maintaining health in chickens. However, how inulin elicits its effects on members of the intestinal microbiota is unknown, even though their importance for energy metabolism and the health of chickens is well documented. A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens. We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6–52.1% of total glycoside hydrolase genes and 34.6–47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved. Inulin and bacitracin inclusion in the basal diet did not change significantly the composition or diversity of these CMOs. Inulin supplementation at three levels promoted the activities of Bacteroides, Prevotella and Bifidobacterium, and 2% level appears to be the most optimal dosage for bifidobacterial activity.

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Dietary phosphate equivalence of four forms of Pi contrasted with a novel microbial phytase from Citrobacter braakii in broiler chickens

Animal Production Science ◽

10.1071/an14489 ◽

2015 ◽

Vol 55 (9) ◽

pp. 1145 ◽

Cited By ~ 4

Author(s):

A. J. Cowieson ◽

F. Fru-Nji ◽

O. Adeola

Keyword(s):

Broiler Chickens ◽

Potassium Phosphate ◽

Basal Diet ◽

Positive Control ◽

Negative Control ◽

Microbial Phytase ◽

Dietary Phosphate ◽

Bone Mineralisation ◽

Tibia Ash ◽

Total P

Two 21-day studies were conducted with broilers to evaluate the efficacy of a bacterial 6-phytase from Citrobacter braakii and compare four Pi sources. The four sources were phosphates of monocalcium (MCP), dicalcium (DCP), tricalcium (TCP) with potassium phosphate (KH2PO4) acting as a ‘positive control’ reference. In each study, 336 4-day-old male birds (Ross 708) were blocked based on initial bodyweight (BW) and randomly allotted to one of seven diets with six replicate cages of eight birds each. Access to experimental diets and water from Days 4 to 25 post-hatching was ad libitum. In the first study, the seven diets were: (1) a low-P negative control (NC) corn-soybean meal basal diet formulated to contain crude protein (CP), Ca, total P, and non-phytate P (nPP) at 218, 9.0, 4.5, and 2.0 g/kg, respectively; (2) NC plus 0.75 g Pi from KH2PO4/kg; (3) NC plus 0.75 g Pi from MCP/kg; (4) NC plus 0.75 g Pi from DCP/kg; (5) NC plus 0.75 g Pi from TCP/kg; (6) NC plus phytase at 500 FYT/kg; (7) NC plus phytase at 1000 FYT/kg. Feeding the low-P NC diet reduced (P < 0.01) BW gain (BWG), feed intake (FI), and tibia ash. Supplementing the NC with Pi or phytase linearly improved (P < 0.01) BWG, FI, and tibia ash. Supplementing the NC with 0.75 g/kg Pi from MCP, DCP, or TCP were equipotent in improving BWG and FI; however, percent tibia ash was higher (P < 0.05) in birds fed MCP than either DCP or TCP. The second study was similar to the first study except that Ca, total P, and nPP in the NC were reduced to 7.0, 4.2, and 1.8 g/kg, respectively; and Pi from MCP, DCP, and TCP were reduced to 0.6 g/kg. Similar to observations in the first study, the low-P NC diet reduced (P < 0.01) BWG, FI, and tibia ash; and supplementing the NC with Pi or phytase linearly improved (P < 0.01) BWG, FI, P digestibility and tibia ash. Furthermore, supplementing the NC with 0.75 g Pi from MCP, DCP, or TCP per kg diet were equipotent in improving BWG, FI, and tibia ash though MCP resulted in superior (P < 0.05) retention of P compared with TCP. Results from both studies showed that the phytase was efficacious in releasing phytate-P for growth and bone mineralisation in chickens compensating, at least, the spared Pi, and that Pi sources commonly used in formulating diets of chickens may be different in their potential to supply digestible P.

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Effect of two microbial phytase preparations on phosphorus utilisation in broilers fed maize-soybean meal based diets

Agricultural and Food Science ◽

10.23986/afsci.5675 ◽

2001 ◽

Vol 10 (1) ◽

pp. 19-26 ◽

Cited By ~ 1

Author(s):

J. VALAJA ◽

S. PERTTILÄ ◽

K. PARTANEN

Keyword(s):

Aspergillus Niger ◽

Trichoderma Reesei ◽

Soybean Meal ◽

Broiler Chickens ◽

Control Diet ◽

Basal Diet ◽

Positive Control ◽

Negative Control ◽

Inorganic P ◽

Microbial Phytase

The present study was carried out to determine the effect of two microbial phytases, Aspergillus niger (FINASEâ FP-500, 291 PU (phytase units)/g) and Trichoderma reesei phytase (FINASEâ P, 5880 PU/g) on phosphorus (P) and calcium (Ca) utilisation and ileal P and Ca digestibility in broiler chickens fed diets based on maize and soybean meal. A total of 96 Ross broiler chickens housed four birds to a cage were used. Four dietary treatments consisted of a positive control supplemented with dicalcium phosphate (17 g/kg), a negative control without inorganic P, basal diet without inorganic P supplemented with Aspergillus niger phytase (2.6 g/kg) and basal diet without inorganic P supplemented with Trichoderma reesei phytase (0.13 g/kg). Both phytases provided 750 PU/kg feed. P retention per unit intake was lowest and P excretion highest in birds fed the positive control diet with inorganic P (P

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Antibiotic-Induced Dysbiosis of Microbiota Promotes Chicken Lipogenesis by Altering Metabolomics in the Cecum

Metabolites ◽

10.3390/metabo11080487 ◽

2021 ◽

Vol 11 (8) ◽

pp. 487

Author(s):

Tao Zhang ◽

Hao Ding ◽

Lan Chen ◽

Yueyue Lin ◽

Yongshuang Gong ◽

...

Keyword(s):

16S Rrna ◽

Gut Microbiota ◽

High Performance ◽

Fat Deposition ◽

16S Rrna Sequencing ◽

Oral Antibiotics ◽

Ms Analysis ◽

Rrna Sequencing ◽

Cecal Microbiota ◽

Metabolite Network

Elucidation of the mechanism of lipogenesis and fat deposition is essential for controlling excessive fat deposition in chicken. Studies have shown that gut microbiota plays an important role in regulating host lipogenesis and lipid metabolism. However, the function of gut microbiota in the lipogenesis of chicken and their relevant mechanisms are poorly understood. In the present study, the gut microbiota of chicken was depleted by oral antibiotics. Changes in cecal microbiota and metabolomics were detected by 16S rRNA sequencing and ultra-high performance liquid chromatography coupled with MS/MS (UHPLC–MS/MS) analysis. The correlation between antibiotic-induced dysbiosis of gut microbiota and metabolites and lipogenesis were analysed. We found that oral antibiotics significantly promoted the lipogenesis of chicken. 16S rRNA sequencing indicated that oral antibiotics significantly reduced the diversity and richness and caused dysbiosis of gut microbiota. Specifically, the abundance of Proteobacteria was increased considerably while the abundances of Bacteroidetes and Firmicutes were significantly decreased. At the genus level, the abundances of genera Escherichia-Shigella and Klebsiella were significantly increased while the abundances of 12 genera were significantly decreased, including Bacteroides. UHPLC-MS/MS analysis showed that antibiotic-induced dysbiosis of gut microbiota significantly altered cecal metabolomics and caused declines in abundance of 799 metabolites and increases in abundance of 945 metabolites. Microbiota-metabolite network revealed significant correlations between 4 differential phyla and 244 differential metabolites as well as 15 differential genera and 304 differential metabolites. Three metabolites of l-glutamic acid, pantothenate acid and N-acetyl-l-aspartic acid were identified as potential metabolites that link gut microbiota and lipogenesis in chicken. In conclusion, our results showed that antibiotic-induced dysbiosis of gut microbiota promotes lipogenesis of chicken by altering relevant metabolomics. The efforts in this study laid a basis for further study of the mechanisms that gut microbiota regulates lipogenesis and fat deposition of chicken.

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Ranitidine as an alcohol dehydrogenase inhibitor in acute methanol toxicity in rats

Human & Experimental Toxicology ◽

10.1177/0960327109353777 ◽

2009 ◽

Vol 29 (2) ◽

pp. 93-101 ◽

Cited By ~ 8

Author(s):

Amal A El-Bakary ◽

Sahar A El-Dakrory ◽

Sohayla M Attalla ◽

Nawal A Hasanein ◽

Hala A Malek

Keyword(s):

Alcohol Dehydrogenase ◽

High Performance ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Sprague Dawley ◽

Blood Samples ◽

Methanol Poisoning ◽

Sprague Dawley Rats

Methanol poisoning is a hazardous intoxication characterized by visual impairment and formic acidemia. The therapy for methanol poisoning is alcohol dehydrogenase (ADH) inhibitors to prevent formate accumulation. Ranitidine has been considered to be an inhibitor of both gastric alcohol and hepatic aldehyde dehydrogenase enzymes. This study aimed at testing ranitidine as an antidote for methanol acute toxicity and comparing it with ethanol and 4-methyl pyrazole (4-MP). This study was conducted on 48 Sprague-Dawley rats, divided into 6 groups, with 8 rats in each group (one negative control group [C1], two positive control groups [C2, C3] and three test groups [1, 2 and 3]). C2, C3 and all test groups were exposed to nitrous oxide by inhalation, then, C3 group was given methanol (3 g/kg orally). The three test groups 1, 2 and 3 were given ethanol (0.5 g/kg orally), 4-MP (15 mg/kg intraperitoneally) and ranitidine (30 mg/kg intraperitoneally), respectively, 4 hours after giving methanol. Rats were sacrificed and heparinized, cardiac blood samples were collected for blood pH and bicarbonate. Non-heparinized blood samples were collected for formate levels by high performance liquid chromatography. Eye balls were enucleated for histological examination of the retina. Ranitidine corrected metabolic acidosis (p = .025), decreased formate levels (p = .014) and improved the histological findings in the retina induced by acute methanol toxicity.

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Dietary hybrid phytase and carbohydrases on nutrient digestibility and bone quality of broiler chickens

Pesquisa Agropecuária Brasileira ◽

10.1590/s1678-3921.pab2021.v56.01668 ◽

2021 ◽

Vol 56 ◽

Author(s):

Caroline Bavaresco ◽

Everton Luis Krabbe ◽

Diego Surek ◽

Edenilse Gopinger ◽

Fernando Nicolas Martinez ◽

...

Keyword(s):

Bone Quality ◽

Broiler Chickens ◽

Basal Diet ◽

Positive Control ◽

Nutrient Digestibility ◽

Metabolizable Energy ◽

Available P ◽

Available Phosphorus ◽

Poultry Diets

Abstract The objective of this work was to evaluate the effect of the use of hybrid phytase, alone or combined with carbohydrases, in poultry diets with nutritional reductions of calcium, available phosphorus, and metabolizable energy on the nutrient digestibility and bone quality of broiler chickens. A total of 1,875 broilers were distributed in five treatments in a completely randomized design, with 15 replicates of 25 chickens each. The treatments consisted of a positive control feed (T1) and of four negative controls (T2 to T5): T1, basal diet (BD) with corn and soybean; T2 and T3, BDs with reductions of 70 and 100 kcal kg-1 metabolizable energy, respectively, and both with reductions of 0.16% Ca and 0.15% available P; and T4 and T5, BDs with the same nutritional reductions, but supplemented with enzymes, i.e., T4 = T2 + 500 phytase units (FTU) per kilogram and T5 = T3 + 500 FTU kg-1 + 560 xylanase units (TXU) per kilogram + 250 glucanase units (TGU) per kilogram. The use of 500 FTU kg-1 hybrid phytase in pelleted corn-soybean meal diets allows a good digestive performance by broilers and replaces the nitrogen-corrected apparent metabolizable energy at 70 kcal kg-1, as well as 0.16% Ca and 0.15% available P.

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Cholesterol-lowering activity of lactic acid bacteria and yeast when used as probiotics in laying quail (Coturnix coturnix Japonica)

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.45.4.305-319 ◽

2020 ◽

Vol 45 (4) ◽

pp. 305-319

Author(s):

L. Istiqomah ◽

A. A. Sakti ◽

A. Sofyan ◽

H. Herdian ◽

A. S. Anggraeni

Keyword(s):

Density Lipoprotein ◽

Basal Diet ◽

Positive Control ◽

Negative Control ◽

Production Performance ◽

Coturnix Japonica ◽

Coturnix Coturnix Japonica ◽

Feed Conversion ◽

Coturnix Coturnix ◽

Laying Quail

This study aimed to evaluate the administration of Lactobacillus plantarum AKK-30 and Saccharomyces cerevisiae B-18 as probiotics in reducing cholesterol of blood, egg, and meat and also the production performance of laying quail (Coturnix coturnix Japonica). A number of 600 birds of the twenty one-day-old quails were distributed in a completely randomized design with 5 treatments and 6 replications (initial BW= 101.35 ± 1.64 g). Treatments were as follows: A = negative control (basal diet without probiotic), B = 1% of L. plantarum AKK-30 (107 cfu/g), C = 1% of S. cerevisiae B-18 (106 cfu/g), D = 1% of a probiotic consortium, E = positive control (commercial probiotic). Parameters observed were blood biochemistry profiles, product quality, and production performance. The results revealed that administration of 1% S. cerevisiae (C) resulted the lowest cholesterol in blood (101.75 mg/dL) and egg (9.44 mg/g) and while administration of 1% L. plantarum(B) increased meat protein and decreased fat content (P<0.05). Level of blood triglycerides, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) were not different among treatments. Probiotic treatments did not affect the growth performance, quail day production (QDP), feed conversion ratio (FCR) and egg uniformity. However, probiotic increased diameter of egg yolk (P<0.05). It was concluded that administration of single probiotic L. plantarum AKK-30 or S. cerevisiae B-18 improves the quality of laying quail products.

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PSIV-B-21 Broilers as an animal model to assess different copper, manganese and zinc mineral sources on absorption

Journal of Animal Science ◽

10.1093/jas/skab235.717 ◽

2021 ◽

Vol 99 (Supplement_3) ◽

pp. 394-394

Author(s):

Angela R Boyer ◽

Heaven Roberts ◽

Dennis Nuzback ◽

Miriam Garcia

Keyword(s):

Repeated Measures ◽

Basal Diet ◽

Positive Control ◽

Negative Control ◽

Mineral Absorption ◽

Study Objective ◽

T1 And T2 ◽

Status Data ◽

The Impact ◽

Mineral Sources

Abstract The study objective was to evaluate absorption differences between different sources of Copper (Cu), Manganese (Mn) and Zin (Zn) in broiler diets. Cobb500 chicks (n = 130, 0-d old) were housed in colony brooders and offered basal diet and water ad libitum for eight days. Following the transition phase, 96 straight-run chicks were randomly assigned to one of 48 battery cages (2 chicks/cage; 12 cages/treatment) and offered one of three soy-corn based diets for three weeks: Negative Control (NC; Basal diet at 6 mg/kg Cu, 35 mg/kg Mn, and 36 mg/kg Zn); Positive Control (PC; NC + 15 mg/kg Cu, 100 mg/kg Mn, and 100 mg/kg Zn from Sulfate sources); Treatment 1 (T1; NC + 15 mg/kg Cu, 100 mg/kg Mn, and 100 mg/kg Zn from hydroxychloride Product 1); and Treatment 2 (T2; NC + 15 mg/kg Cu, 100 mg/kg Mn, and 100 mg/kg Zn from hydroxychloride sources Product 2). Week three birds were bled and dissected to obtain liver, breast and femur samples for mineral absorption status. Data were analyzed using RV3.6.1 procedures with non-repeated measures. Plasma and breast tissue were not (P ≥ 0.05) impacted by mineral source, however numerical increases were reported in PC, T1 and T2 over NC. Liver Cu and Zn concentrations were non-significant (P ≥ 0.05) for all treatments. However liver Mn concentrations increased (P ≤ 0.05) in Pc and T1. Copper bone concentrations were not (P ≥ 0.05) affected, but an increase (P ≤ 0.05) in Mn and Zn was reported for PC, T1 and T2 versus NC. These results indicate increased absorption occurs when mineral sources are adequately supplemented beyond basal diet levels and that source may impact absorption rate. Additional research is needed to address the absorption ability of different mineral sources to better understand the impact on bird performance.

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PSVI-1 Improvement of gut morphology and meat quality of broiler chickens by encapsulated citral and cinnamaldehyde

Journal of Animal Science ◽

10.1093/jas/skz258.401 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 195-195

Author(s):

Chongwu Yang ◽

Janghan Choi ◽

Marion Mogire ◽

Argenis Rodas-González ◽

Moussa S Diarra ◽

...

Keyword(s):

Growth Performance ◽

Meat Quality ◽

Ph Value ◽

Basal Diet ◽

Positive Control ◽

Negative Control ◽

Intestinal Morphology ◽

Post Mortem ◽

Gut Morphology

Abstract This study investigated the effects of encapsulated citral (CIT) and cinnamaldehyde (CIN) on growth performance, intestinal morphology and meat quality in broilers. A total of 320 0-day-age male broilers (Cobb 500) were allocated 32 pens with 8 pens per treatment. The treatments included: 1) Corn-wheat-SBM basal diet (negative control); 2) basal diet with 30 ppm avilamycin premix (positive control); 3) basal diet with 50 ppm encapsulated CIT and CIN (CCL); 4) basal diet with 100 ppm encapsulated CIT and CIN (CCH). There were no significant differences between treatments in growth performance (P > 0.05) at each feeding stage. Additionally, birds had similar relative organ weights of heart, liver, spleen and bursa (P > 0.05). The higher ratios of villus height to crypt depth (VCR; P ≤ 0.05) were detected in broilers fed either CCL or CCH, with an average being 14.67 and 15.13 in the duodenum, and 15.13 and 13.58 in the jejunum, respectively. Regarding meat quality, a higher pH value (pH = 6.21) in the breast was observed in 24-h post mortem chickens fed CCL (P ≤ 0.05). No significant differences of pH were detected in the breast in 96-h post mortem birds. The breast color (redness, yellowness, and lightness) in either 24-h or 96-h post mortem chickens was not significantly different among treatments (P > 0.05). No change in purge loss (%) was observed in the breast after 48 h. The severity of white striping (WS) or woody meat (WB) was higher in the positive control (WS = 0.58; WB = 0.15) than in other treatments (P ≤ 0.05). In conclusion, dietary treatment with encapsulated CIT and CIN improved gut morphology and meat quality of broilers by increasing VCR in the small intestine and reducing the severity of WS/WB in the breast, respectively, without affecting growth performance.

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