Effects of Ultraviolet-C Pretreatment on Sugar Metabolism in Yellow Peaches during Shelf Life

To investigate the influence of ultraviolet-C (UVC) radiation pretreatment on the sugar metabolism of yellow peaches (cv. Beinong2 × 60–24–7) during storage, the concentrations of soluble sugar (sucrose, fructose, glucose, and sorbitol), and related gene expression were determined. During UVC pretreatment, peaches were subjected to a dose of 4 kJ·m−2 when they were placed at 15 cm under a UVC lamp tube for 10 minutes at 25 °C. Then, they remained at 15 ± 2 °C for 10 days. Peaches stored at 15 ± 2 °C immediately after picking were used as the control group (CG). UVC pretreatment reduced the ethylene production rate and resulted in a significant increase in the accumulation of sucrose during days 2 to 8 of the storage period, followed by a lower concentration of fructose and glucose and the upregulation of PpaSS1. The expression levels of PpaSPS2, PpaSS1, and PpaST3 were significantly correlated with fructose concentration, and those of PpaSPS2 and PpaST2 were significantly correlated with glucose concentration. The enzyme activity of sucrose phosphate synthase (SPS) was positively correlated with PpaSPS2, PpaSS2, and PpaST2. The enzyme activities of sucrose synthase (SS), acid invertase (AI), and neutral invertase (NI) were positively correlated with PpaSS1, PpaST1, and Ppani, respectively. Expressions of PpSPS1 and PpSPS2 in UVC-pretreated peaches were upregulated on storage days 8 and 2, and there was a UVC-induced peak in SPS activity on storage days 4 and 8, which resulted in the rapid accumulation of sucrose. UVC pretreatment could upregulate the gene expression of PpaSS1 on day 2, which could improve and maintain the quality of peaches for consumption.

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Effect of Gelatin Coated Rosemary Extract (Rosmarinus Officinalis L.) on the Quality of Refrigerated Duck Meat

Agricultural Science Digest - A Research Journal ◽

10.18805/ag.d-180 ◽

2019 ◽

Vol 39 (04) ◽

Author(s):

Kimia Banikarimi K Rai ◽

Habibollah Mirzaei K Basu ◽

Mohammad Farsi K Basu

Keyword(s):

Storage Period ◽

Edible Coating ◽

Water Holding Capacity ◽

Rosemary Extract ◽

Control Group ◽

Total Volatile ◽

Psychrotrophic Bacteria ◽

Rosmarinus Officinalis L ◽

Water Holding

The effect of edible coating of gelatin (1, 2 and 3%) containing aqueous extract of rosemary (1% and 2%) on physiochemical (pH, total volatile nitrogen, peroxide values and water holding capacity) and microbiological (psychrotrophic) properties of duck fillet were evaluated. The pH of coated samples were significantly (plessthan0.05) lower than that of untreated sample. TVN (total volatile nitrogen) value increased with storage period in all the samples but coatings significantly decreased production of TVN compared to the control. Also the coatings decreased lipid oxidation of the meat as rosemary postponed primary oxidation of fillet, while this parameter in control group was sharply increased by time. WHC (water holding capacity) increased significantly (plessthan0.05) with storage period and level coatings. Coating had a significant reducing effect on growth of psychrotrophic bacteria during 9 days at 4°C. In conclusion, gelatin edible coating enriched with rosemary could retard chemical and microbial reactions related to spoilage of meat during refrigerated storage.

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High Carbon Dioxide Treatment Modulates Sugar Metabolism and Maintains the Quality of Fresh-Cut Pear Fruit

Molecules ◽

10.3390/molecules25184261 ◽

2020 ◽

Vol 25 (18) ◽

pp. 4261

Author(s):

Di Wang ◽

Quan Ma ◽

Tarun Belwal ◽

Dong Li ◽

Wenxuan Li ◽

...

Keyword(s):

Carbon Dioxide ◽

Fruit Quality ◽

Soluble Sugars ◽

Sugar Metabolism ◽

Sucrose Phosphate Synthase ◽

Soluble Solids ◽

Related Enzyme ◽

Fresh Cut ◽

Carbon Dioxide Co2

The purpose of this study is to explore the effect of 10% carbon dioxide (CO2) on the fruit quality and sugar metabolism of fresh-cut pear during storage. The results indicated that carbon dioxide treatment maintained fruit quality by delaying the decline of firmness and promoting the accumulation of total soluble solids (TSS). Moreover, carbon dioxide enhanced activities of sucrose synthase (SS), and sucrose phosphate synthase (SPS). The activities of amylase, acid invertase (AI), neutral invertase (NI), SS-cleavage, fructokinase (FK), hexokinase (HK), sorbitol oxidase (SOX), NAD-dependent sorbitol dehydrogenase (NAD-SDH), and NADP-SDH in CO2-treated fruit were inhibited. Expression levels of key genes were found to correspond with the related enzyme activities. As a result, the accumulation of glucose, fructose, sorbitol, and sucrose were accelerated by CO2, which were 12.58%, 13.86%, 24.7%, and 13.9% higher than those of the control at the end of storage, respectively. The results showed that CO2 could maintain the quality of fresh-cut pears by regulating the conversion of various sugar components to enhance soluble sugars content.

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Quality of kohlrabi stems (Brassica oleracea var. gongylodes L.) kept in cold storage

Folia Horticulturae ◽

10.2478/v10245-011-0016-3 ◽

2011 ◽

Vol 23 (2) ◽

pp. 107-110 ◽

Cited By ~ 2

Author(s):

Maria Gawęda ◽

Zofia Nizioł-Łukaszewska

Keyword(s):

Ascorbic Acid ◽

Brassica Oleracea ◽

Cold Storage ◽

Dry Matter ◽

Sugar Content ◽

Soluble Sugar ◽

Soluble Sugars ◽

Storage Period ◽

Dry Matter Content

Quality of kohlrabi stems (Brassica oleracea var. gongylodes L.) kept in cold storage Two green kohlrabi cultivars, ‘White Delikates’ and ‘Korist’ F1, were kept in cold storage at a temperature of 2°C and a relative humidity of 95%. Natural mass losses were measured at monthly intervals and dry matter content, soluble sugars, L-ascorbic acid and isothiocyanates were analysed. During five months of storage, very low losses of kohlrabi mass were detected. The decrease in dry matter during that time was between 15 and 18%. After a brief increase, soluble sugar content decreased during storage, and in March, 50% of the initial sugar content was calculated for ‘Delikates’ kohlrabi flesh and 65% for ‘Korist’. L-ascorbic acid was well preserved in the kohlrabi, since 90% remained after storage was completed. The isothiocyanate content changed little and the vegetable remained a good source of these compounds throughout the storage period.

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The impact of different levels of nisin as a biopreservative agent on the chemical, sensory and microbiological quality of vacuum-packed sea bass (Dicentrarchus labrax) fillets stored at 4 ± 2 °C

Grasas y Aceites ◽

10.3989/gya.1261192 ◽

2021 ◽

Vol 72 (2) ◽

pp. e401

Author(s):

Y. Ucar ◽

Y. Ozogul ◽

F. Ozogul ◽

M. Durmus ◽

A.R. Kösker ◽

...

Keyword(s):

Shelf Life ◽

Microbiological Quality ◽

Dicentrarchus Labrax ◽

Storage Period ◽

Sea Bass ◽

Control Group ◽

Gram Positive Bacteria ◽

C Storage ◽

The Impact

Nisin is produced by Lactococcus lactis subsp. lactis and is also known as an antimicrobial agent especially effective against gram-positive bacteria. It has long been used as a preservative in foods and beverages and is generally regarded as safe (GRAS). In the present work, the effects of different concentrations of nisin (0.2, 0.4 and 0.8%) on the sensory, chemical and microbiological quality and shelf-life of vacuum-packed sea bass (Dicentrarchus labrax) fillets were investigated during chilled (4 ± 2 °C) storage. The sensory points for raw and cooked fillets increased with time during the storage period (p < 0.05). The control group, with scores of 9.08, was rejected by panelists on day 12; whereas nisin-treated groups were rejected on day 14 with scores ranging from 9.00-9.17 score. As a result of chemical analyses, lower values (p < 0.05) were obtained from the nisin groups with low oxidative rancidity. Moreover, nisin inhibited microbial growth, which shows antimicrobial activity. Consequently, it was concluded that the application of nisin (especially 0.8%) preserved the organoleptic quality and extended the shelf-life of sea bass fillets.

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Effects of Melatonin Treatment of Postharvest Pear Fruit on Aromatic Volatile Biosynthesis

Molecules ◽

10.3390/molecules24234233 ◽

2019 ◽

Vol 24 (23) ◽

pp. 4233 ◽

Cited By ~ 3

Author(s):

Jianlong Liu ◽

Hanting Liu ◽

Ting Wu ◽

Rui Zhai ◽

Chengquan Yang ◽

...

Keyword(s):

Gene Expression ◽

Consumer Satisfaction ◽

Storage Period ◽

Hydroperoxide Lyase ◽

Pear Fruit ◽

Expression Of Genes ◽

Pyrus Communis L ◽

Genes Encoding ◽

Alcohol Acyltransferase

Aroma affects the sensory quality of fruit and, consequently, consumer satisfaction. Melatonin (MT) is a plant growth regulator used to delay senescence in postharvest fruit during storage; however, its effect on aroma of pear fruit remains unclear. In this study, we assessed the effects of 0.1 mmol L−1 MT on volatiles and associated gene expression in the fruit of pear cultivars ‘Korla’ (Pyrus brestschneideri Rehd) and ‘Abbé Fetel’ (Pyrus communis L.). MT mainly affected the production of C6 aromatic substances in the two varieties. In ‘Korla’, MT inhibited expression of PbHPL, and reduced hydroperoxide lyase (HPL) activity and content of hexanal and (E)-hex-2-enal. In contrast, MT inhibited activity of lipoxygenase (LOX), reduced expression of PbLOX1 and PbLOX2, promoted PbAAT gene expression, increased alcohol acyltransferase (AAT) activity, and increased propyl acetate, and hexyl acetate content in ‘Abbé Fetel’ that similarly led to the reduction in content of hexanal and (E)-hex-2-enal. Content of esters in ‘Abbé Fetel’ pear increased with increasing postharvest storage period. Although mechanisms differed between the two varieties, effects on aroma volatiles mediated by MT were driven by expression of genes encoding LOX, HPL, and AAT enzymes.

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The Influence on Storage Quality of Pleurotus Eryngii by 1-Methylcyclopropene Treatment

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.675-677.1685 ◽

2014 ◽

Vol 675-677 ◽

pp. 1685-1690 ◽

Cited By ~ 1

Author(s):

Chun Yan Zhao ◽

Li Wei Zhao ◽

Yu Zhen Pi ◽

Xi Wu ◽

Fang Fei Ma

Keyword(s):

Weight Loss ◽

Loss Rate ◽

Fruit Body ◽

Storage Period ◽

Pleurotus Eryngii ◽

Control Group ◽

Processing Conditions ◽

Storage Quality ◽

Weight Loss Rate

The paper is concerned with 1-MCP effect on post-harvest Pleurotus eryngii weight loss rate, polyphenol oxidase (PPO) activity and fruit body microstructure at different storage stage.The results showed that compared with control group, the weight loss rate and PPO activity were decreased by 5.94% and 37.38% ,and its microstructure had least change after 14d when treated with 0.3µL/L 1-MCP.The weight loss rate and PPO activity also decreased by 5.42% and 33.98% respectively with least change in fruit body microstructure after 14d when treated with 1-MCP for 24h. It proved that the optimal 1-MCP processing concentration is 0.3µL/L and the most appropriate processing time is 24h. These processing conditions slowed down the weight loss rate and browning level ,also reduced the change of fruit bodies microstructure and extended storage period

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Effect of Broilers Chicken Diet Supplementation with Natural and Acidified Humic Substances on Quality of Produced Breast Meat

Animals ◽

10.3390/ani11041087 ◽

2021 ◽

Vol 11 (4) ◽

pp. 1087

Author(s):

Marek Hudák ◽

Boris Semjon ◽

Dana Marcinčáková ◽

Lukáš Bujňák ◽

Pavel Naď ◽

...

Keyword(s):

Lipid Oxidation ◽

Humic Substances ◽

Broiler Chickens ◽

Storage Period ◽

Basal Diet ◽

Control Group ◽

Total Protein Content ◽

Breast Meat ◽

Broiler Diet

This study was conducted to examine the effect of two humic substances (HS) supplemented in broilers’ diet on the breast meat quality of broiler chickens. In this experiment, 120 pieces of one-day-old COBB500 broiler chickens were used. Broilers were divided into three groups, each containing 40 birds with three replications (13, 13, and 14 per one pen). Fattening lasted 38 days. The first experimental diet was supplemented with 0.7% of HS (HS0.7) and the second was enriched with 0.7% of acidified HS (HSA0.7). The control group of broilers (C) was fed a basal diet without the addition of any supplements. HS0.7 samples had the highest total protein content and the lowest content of fat (p < 0.01). The effects of broiler diet and storage had a significant impact on the pH of breast samples, p < 0.001 and p < 0.05, respectively. Supplementation of HS in broiler diet positively affected the lipid oxidation of breast meat samples, as indicated by the lower malondialdehyde content (p < 0.05). HSA0.7 samples had significantly better juiciness after the storage period (p < 0.001). The quality of meat produced with supplementation of HS in broilers’ feed can be considered as valuable in human nutrition due to improved protein and fat content with decreased lipid oxidation.

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Evolution of Merchantability during Storage of Maize Triple Bagged Containing Biopesticides (Lippia multiflora and Hyptis suaveolens)

European Journal of Nutrition & Food Safety ◽

10.9734/ejnfs/2019/v11i430175 ◽

2020 ◽

pp. 274-283

Author(s):

Yao Vanessa Gaël ◽

Konan K. Constant ◽

Niamketchi G. Leonce ◽

A. Aka Boigny Ange ◽

Adama Coulibaly ◽

...

Keyword(s):

Cote D'ivoire ◽

Côte D’Ivoire ◽

Storage Period ◽

Control Group ◽

Cote D’Ivoire ◽

Lippia Multiflora ◽

Côte D'ivoire ◽

Hyptis Suaveolens ◽

Maize Grain

The aim of study was to evaluate merchantability quality of stored maize in triple bagging with biopesticides. Maize grains were collected in March 2016 in the north of Côte d’Ivoire. The fresh leaves of Lippia multiflora and Hyptis suaveolens were collected and dried in sunlight for 7 days in the center of Cote d'Ivoire. Triple bags were bought in Abidjan market. All this material was sent to the Laboratory of Biochemistry and Food Sciences, Félix Houphouët-Boigny University, Côte d’Ivoire, to perform the experiment. Ten treatments were obtained for the experimentation. The first treatment was conservation of 50 kg of maize grain in a polypropylene bag. The second treatment was conservation of 50 kg of maize grain in a PICS bag. The other eight treatments were carried out with PICS bags each containing 50 kg of maize grain and different proportions of chopped leaves Lippia multiflora and Hyptis suaveolens. A central composite design was used for sample constitution. Thus, a control group with polypropylene bags (TPPB0), a control group in PICS bags without biopesticides (TPB0) and 8 experimental lots of triple bags noted TB1 containing 0.625 kg L. multiflora and 0.625 kg H. suaveolens, TB2 with 0.40 kg of L. multiflora and 1.60 kg of H. suaveolens, TB3 with 1.60 kg of L. multiflora and 0.40 kg of H. suaveolens, TB4 with 0.10 kg of L. multiflora and 0.40 kg of H. suaveolens, TB5 with 0.40 kg of L. multiflora and 0.10 kg of H. suaveolens, TB6 with 2.5 kg of L. multiflora and 2.5 kg of H. suaveolens, TB7 with 1.25 kg of L. multiflora and TB8 with 1.25 kg of H. suaveolens have been used. Changes in moisture, damages and weight losses were studied. The results show moisture levels (from 09.02±0.11% to 12.07± 0.06%), weight loss (from 0.49±0.02% to 2.54±0.07%) and damage (from 0.99±0.02% to 3.96± 0.01%), corn stored in triple bagged bags with different proportions of biopesticide were significantly lower than those recorded in the Polypropylene woven sample bag (TPPB0) and in the triple bagged control bag during the storage period. The results obtained indicate stability in the quality of maize stored for 18 months in triple bagged bags containing different proportions of leaves of L. multiflora and H. suaveolens. A proportion of 5% of the mixture of leaves of L. multiflora and H. suaveolens (2.5 kg of L. multiflora and 2.5 kg of H. suaveolens) in triple bagged bags is recommended for a better preservation of the merchantability of the stored maize grains kernels. Storage of maize grains in PICS bags with the leaves of L. multiflora and H. suaveolens appears as a method of effective and inexpensive conservation to ensure the merchantability quality of maize.

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Effects of Resveratrol Supplementation on the Motility, Structural Integrity, and Mitochondrial Function of Freeze-Thawed Dog Sperm

10.20944/preprints202012.0821.v1 ◽

2020 ◽

Author(s):

Seonggyu Bang ◽

Ahmad Yar Qamar ◽

Bereket Molla Tanga ◽

Xun Fang ◽

Jongki Cho

Keyword(s):

Gene Expression ◽

Plasma Membrane ◽

Structural Integrity ◽

Human Sperm ◽

Control Group ◽

Mitochondrial Activity ◽

Sperm Analysis ◽

Damage Repair ◽

Trans Stilbene

Antioxidants have multiple protective roles in cells and can be used as a supplement to protect cells against cryopreservation-induced detrimental effects, including protecting sperm fertility quality. The antioxidant resveratrol (3,5,4-trihydroxy-trans-stilbene; RSV) has been shown to be a protective supplement for the cryopreservation of animal sperm, including human sperm. In this study, we assessed the effect of RSV supplementation on canine sperm cryopreservation. Semen was collected from four dogs and the effect of different concentrations of RSV (0, 100, 200, and 400 µM) on post-thaw quality of sperm was examined. After thawing, sperm motility was assessed using computer aided sperm analysis, and the structural integrity of the plasma membrane, acrosome, and chromatin were examined, as well as mitochondrial activity and gene expression were assessed. Dog sperm cryopreserved with 200 µM RSV showed significant improvement in motility and viability following thawing compared with that of the control group (p &lt; 0.05). Moreover, RSV-supplemented samples showed significantly higher numbers of sperm with an intact plasma membrane, active mitochondria, and structural integrity of acrosomes and chromatin than that of control samples (p &lt; 0.05). Furthermore, gene expression showed that RSV supplemented samples showed lower expression of pro-apoptotic (BAX) oxidative stress-related (ROMO1) and oxidative induced DNA damage repair (OGG1) whereas higher expression levels of anti‐apoptotic (BCL2) protamine-2 (PRM2), protamine-3 (PRM3) and sperm acrosome‐associated (SPACA3) genes than control. Our results suggest that RSV, at its optimum concentration, can be efficiently used as an alternative antioxidant in the cryopreservation of dog sperm.

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Analysis of the Application of Fungicide and Ethylene Absorbent for Extended Storage Life of Kyoho Grapes at Room Temperature

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.731.374 ◽

2015 ◽

Vol 731 ◽

pp. 374-380

Author(s):

Zhen Lu Zhang ◽

Dong Li Li ◽

Wen Cai Xu ◽

Hui Ye Duan ◽

Ya Bo Fu

Keyword(s):

Vitis Vinifera ◽

Room Temperature ◽

Storage Period ◽

Storage Life ◽

Control Group ◽

Vitis Vinifera L ◽

Table Grapes ◽

Combined Use ◽

Physiological Quality

The Kyoho grape is one of the most important varieties of table grapes (Vitis vinifera L.). In this experiment, we used SO2 germicidal bags and ethylene absorbent released pads to discover the effect on the quality of grapes during the storage period. The results showed that the combined use of germicidal bags and ethylene absorbent released pad were able to maintain the sensory, physicochemical, and physiological quality of grapes at a higher level compared with the control group.

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