scholarly journals Evaluation of the effect of enzymatic hydrolyzate of the bursa of broiler chickens on cytotoxic activity and nonspecific immunity

Vestnik MGTU ◽  
2021 ◽  
Vol 24 (3) ◽  
pp. 259-266
Author(s):  
N. A. Kolberg ◽  
S. A. Leontyeva ◽  
S. L. Tikhonov ◽  
N. V. Tikhonova ◽  
S. V. Shikhalev ◽  
...  

The use of biologically active substances of tissue origin for the creation of biologically active additives and immunomodulatory drugs is an important area of scientific research in the field of nutrition and pharmacology. The aim of the work is to evaluate the effect of enzymatic hydrolysate from the fabricium bag of broiler chickens on the cytotoxicity and nonspecific immunity of mice against the background of experimental salmonella infection using biotechnological methods. For the experiment, three equal experimental groups of white mongrel mice were formed. All laboratory animals were fed daily for seven days with enzymatic hydrolysate at a dose of 750 mg/kg (therapeutic dose), 150 mg/kg (0.2 therapeutic dose) and 3,750 mg/kg (five therapeutic doses). At the same time, the control group of animals received intragastric water in the same volume. 24 hours after the last intake of the enzymatic hydrolysate, the animals were intraperitoneally infected with a culture of Salmonella enteritidis 92. The study reveals the absence of cytotoxic properties and impaired cell viability in cultures L929, J774.1A, HeLa S3, K562, and HST116 against the background of exposure to various concentrations of enzymatic hydrolysate from 0.02 to 10 mg/ml. It should be noted that there is a pronounced cytotoxic effect on MCF-7 tumor cells of the concentration of enzymatic hydrolysate in culture of 5 and 10 mg/ml, which suggests the possibility of using enzymatic hydrolysate for the prevention of tumor diseases. As a result of the experiment, it has been proved that the administration of enzymatic hydrolysate to mice increased the LD50 index and the survival rate of mice infected with the culture of the causative agent of salmonellosis by 1.5 times. Based on the conducted studies, it has been shown that the enzymatic hydrolysate of the lymphoid tissue of broiler chickens does not violate the viability of mammalian cells and does not exhibit cytotoxic properties on the metabolism of healthy mammalian cells, which indicates its safety. In an experiment on mice, the effect of enzymatic hydrolysate of the fabricium bag of broiler chickens on the activation of nonspecific immunity against salmonella infection has been revealed.

Author(s):  
S. A. Shpуnova ◽  
O. A. Yadrishchenskaya ◽  
T. V. Selina ◽  
E. A. Basova

In order to increase the meat productivity of broiler chickens, increase production volumes and reduce the prime-cost of poultry products, it is necessary not only to ensure balanced feeding and compliance with production technology but also to include in the poultry diet non-traditional additives, biologically active substances that contribute to the normalization of metabolic processes and improve the digestibility of feed nutrients. One of these dietary additives is Betulin extracted from birch bark, which has antioxidant and immunomodulatory properties. In this regard, the purpose of the research was to study the influence of compound feed with the introduction of 2 % feed additive based on a synthetic microspherical nanoporous carbon carrier containing biologically active Betulin in its composition on the productivity of broilers. The results of studies on broiler chickens using compound feed with 2 % feed additive have been presented in the article. It has been found that the feed consumption per 1 kg of live weight gain in poultry of the experimental group have been lower by 9,5 % due to better absorption of nutrients, the live weight of broiler chickens of the experimental group at the age of 42 days was 2625,6 g and it was higher than the control group by 3,68 %. The digestibility of crude protein and fat reached 92,73 and 88,03 %, which exceeded the control group by 1,03 and 3,03 abs.%, respectively. It has been found that the use of compound feed with 2 % of the feed additive with the biologically active substance is of practical importance in connection with the increase in the productivity of broilers and the level of profitability of meat production by 8,2 abs.%.


Author(s):  
B. S. Kaloev ◽  
M. O. Ibragimov ◽  
M. M. Shagaipov

The reliability of the results obtained in scientific and economic experiments on the study of the effectiveness of the use of various feed components, including biologically active substances in the rearing of broilers needs to be confi rmed in production conditions. The purpose of the research was to study the joint use of enzyme drugs and lecithin in the rearing of broiler chickens. The results of production experiment on the study of the effectiveness of the joint inclusion of enzyme drugs Sunzyme and Sunfyse 5000 as well as lecithin in the diet of broiler chickens of the cross Ross-308 have been presented in the article. Production tests have been carried out under the conditions of the breeding farm “Achkhoy– Martanovsky” in the Chechen Republic. The duration of the experiment was 45 days. For research on the principle of analog groups two groups have been formed: a control group and an experimental group of 1000 heads in each. The broilers of the control group have been fed compound feed prepared in accordance with the growing period and consisting mainly of grain components of local production. The broilers of the experimental group have been fed the same compound feed, but with the addition of two enzyme drugs Sanzyme and Sanfyse 5000 (100 g/t of feed) as well as lecithin (10 g/kg of feed). The results of the production experiment fully confi rmed the data obtained in the scientific and economic experiment. The positive effect of the joint use of the studied enzyme drugs and lecithin has been manifested in all the studied productive traits. In particular, the increase in live weight of broilers increased by 12,1 %, slaughter yield by 3,5 %, the yield of carcasses of the 1st category by 3,0 %. The main economic indicators also found their confirmation: feed consumption per unit of growth decreased by 11,7 %, additional profit was received in the amount of 27308,2 rubles, the level of profitability of broiler rearing increased by 18,4 %.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 294-294
Author(s):  
Kirill Plemyashov ◽  
Alexander Aristov ◽  
Sergey Semenov ◽  
Natal’ya Kudinova ◽  
Polina Anipchenko ◽  
...  

Abstract Over recent years, the range of biologically active substances aimed at the productivity increase, animals preservation, feed use efficiency, product quality improvement and product safety has been significantly expanded. In this regard, the study of the effectiveness of hepatoprotective farm-produced feed additives in the diets of broiler chickens is of particular interest to science and practice. The broiler chickens pectoral muscles of the control group contained 68–0.08% of water, 32–0.06% of dry matter, 20.45–0.08% of protein, and 1.62–0.09% fat. In a comparative analysis of the obtained data, it may be concluded that the use of the feed additive Hepatron 85% as a hepatoprotector improves the detoxification ability of the liver and promotes the regeneration of the liver cells. Betaine as a lipotropic agent contributes to the fat oxidation, enhances appetite, and adds muscle mass. When determining the chemical composition of the muscles of the leg, thigh, body, wings and neck, it was established that the broiler chickens of the experimental group contained 70–0.07% of water, 30–0.05% of dry matter, 19.7±0.08% of protein and 2.11±0.09% of fat. Using the feed with the additive Hepatron 85% causes a 0.5% increase in protein and 0.18% increase in fat compared to the meat of the broiler chickens on general feed. Thus, the inclusion of the feed additive Hepatron 85% in the ration of broiler chickens instead of 50% of methionine and choline contributes to an increase in average daily gains and meat quality improvement in a number of indicators.


2019 ◽  
Vol 10 (1) ◽  
pp. 43-54 ◽  
Author(s):  
M. Nakphaichit ◽  
S. Sobanbua ◽  
S. Siemuang ◽  
W. Vongsangnak ◽  
J. Nakayama ◽  
...  

Poultry is an important high-quality food and protein source for humans. However, chicken is considered a primary source of foodborne diseases, especially Salmonella Enteritidis infection. Reducing Salmonella contamination in live poultry will thus lower the risk to consumers. Our previous studies reported that Lactobacillus reuteri KUB-AC5 can produce a substance with antimicrobial activity against pathogenic bacteria, especially Salmonella. In vivo testing revealed that this strain greatly influenced the ileal microbiota by improving chicken gastrointestinal health and inhibiting certain pathogenic bacteria. However, its activity against Salmonella in chicken is unknown. This study investigated the effects of the probiotic L. reuteri KUB-AC5 at various concentrations against Salmonella and the microbiota status in the gastrointestinal tract of broiler chickens. Four treatments groups were used: negative-control group (no Salmonella challenge), positive-control group (Salmonella challenge), and 5 or 7 log cfu probiotic supplementation to Salmonella-challenged chickens. The resultant microbial diversities at the growing and finisher stages were not significantly different among the groups (P>0.05). However, a high dosage of KUB-AC5 maintained similar microbial diversity in Salmonella-challenged chickens as observed in the non-challenged group in the early stage. The exposure Salmonella can affect the microbial diversity that consequently contributes to the disease progression in chicken. Low and high dosages of KUB-AC5 eliminated S. Enteritidis from the ileum and caecum at 14, 21 and 35 days of age. A high-dose of KUB-AC5 also enhanced Lactobacillaceae levels in the growing stage in both the ileum and caecum and suppressed Enterobacteriaceae levels in the finisher stage on day 35, whereas these effects were not observed in the low dose of KUB-AC5 or control groups. These results support the potential value of high-dose L. reuteri KUB-AC5 supplementation for three days after hatching in preventing Salmonella infection in chickens.


REPORTS ◽  
2021 ◽  
Vol 2 (336) ◽  
pp. 13-17
Author(s):  
N. N. Kuzmina ◽  
O. Yu. Petrov ◽  
A. K. Karynbayev ◽  
A. S. Alentayev

The purpose of the research was to determine the feasibility and effectiveness of using a natural antioxidant of plant origin – dihydroquercetin (DHA), as a biologically active substance, in combination with the main standard diet of broiler chickens. Scientific and economic experience conducted in production conditions on broiler chickens of the "COBB-500" cross allowed us to objectively establish that the addition of the biologically active substance Dihydroquercetin to mixed feed contributes to the increase in meat productivity and safety of broiler chickens. Feeding of broiler chickens was carried out with full-fledged compound feeds, in accordance with the recommendations of the originator for this cross. In terms of energy nutrition and the content of nutrient active substances, they were the same and differed between the groups in the amount of the additive administered. Chickens of the 1st control group received only feed (basic diet), the 2nd experimental group – advanced antioxidant supplement Dihydroquercetin in the amount of 0.5 g per 100 g of feed, the 3rd – 0.75 g per 100 g of feed, and 4th – 1 g per 100 g of feed. The duration of the experiment was 40 days and to clarify the effectiveness of increasing the duration of the broiler fattening period, it was extended to 60 days. Broiler chickens were reared on the floor, temperature and light conditions, air humidity, feeding and watering front of the bird during the experiment corresponded to the recommended standards. As a result of the conducted studies on the effect of adding optimal levels of biologically active feed additive Dihydroquercetin to the diet of broiler chickens, it was found that during the growing period, a higher coefficient of live weight gain was established in broiler chickens of the 3rd and 4th experimental groups, whose live weight increased by 105.32 and 120.48 times by the age of 40 days, while in broiler chickens of the 2nd experimental group by 102.57 times and the lowest–in control group chickens only in 90.96 times. The selected feed additive can be considered as an alternative to the use of feed antibiotics in the feeding of broiler chickens in industrial poultry farming technology.


Author(s):  
U.I. Kundryukova ◽  
◽  
Ye.V. Shatskikh ◽  
L.I. Drozdova ◽  
◽  
...  

This paper discusses the outcomes of the production experiment carried out on one of the poultry farms in the Sverdlovsk Region and at the departments of the Ural State Agricultural University. The experiment was carried out on cross Ross-308 broiler chickens in the number of 80 female and 80 male chickens with an average live weight of 43 g. During the research, the chickens were housed in different cages; the experiment continued throughout the entire rearing period - 38 days. The control group formed of comparable chickens was fed with the basic diet used on the farm with the nutritional value required for this cross, and during rearing, from the 1st to 10th day, the Albac feed antibiotic (500 g per t) was administered; it was substituted with Fortrazin (600 g t) from the 11th to 30th day. From the first day of rearing to the end of fattening, the trial group received the basic diet but the antibiotic was substituted with an alternative safe growth promoter SafMannan in the amount of 0.5 kg per t of compound feed. Comparative zootechnical indices of the control and trial groups showed that the introduction of the SafMannan supplement into the compound feed did not affect the chicken survival rates, and had a promoting effect on the growth and development of broilers accompanied by live weight gains. Histological examination of the breast and thigh muscle groups of the control and trial chickens did not reveal any pathological processes neither with the addition of feed antibiotics or with the introduction of the biologically active supplement SafMannan; the muscle mass increased due to young un-differentiated muscle fibers that were in the process matu-ration, but the final differentiation of the entire muscle mass by the end of the technological cycle did not occur.


Author(s):  
G. N. Vyayzenen ◽  
S. V. Razaev ◽  
A. G. Vyayzenen ◽  
N. S. Prodanov ◽  
A. E. Barashkov ◽  
...  

In order to save the cost of expensive compound feed, a number of biologically active substances (vitamins and amino acids) and improve the digestibility of nutrients for the first time in poultry meat using laser technology there is a real possibility of “transferring” the studied amino acids (methionine, glycine) and all water-soluble vitamins using a laser beam through the skin in the breast area of broiler chickens. The research has been carried out in Belgrankorm-Veliky Novgorod LLC in the Krestetsky district in the Novgorod region with a large-cage housing method of broiler chickens of the cross Hubbard. The duration of broilers rearing was 39 days, 4 recipes of compound feed based on wheat grain, corn, soybeen meal with the inclusion of fish flour, sunflower oil, corn gluten, sunflower meal, meat and bone meal and mineral additives (monocalcium phosphate, lime flour) have been used. For better assimilation of nutrients and biologically active substances by the body amino acids (methionine, glycine) and water-soluble vitamins Undevit, both individually and in combination with them in a ratio of 1:1 (by weight), in the form of powders passed through an infra-red laser beam in a spatial modulator, which were attached to two emitters (simultaneously) of the “Uzor-2K-Super” laser in the chest area have been used. Broilers of the experimental groups ate feed more often and faster than the herdmates of the control group. Air ozonation in the poultry house and broilers has been carried out in a mobile way using the Rios ozonator of the Decont series (mode 3, with a capacity of 20 g/m3 ). Innovative methods of influencing on the body caused the increase in the intensity of metabolic processes, growth and development, fattening and meat traits, and feed conversion in broilers.


Animals ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 184 ◽  
Author(s):  
Daniel Hernandez-Patlan ◽  
Bruno Solís-Cruz ◽  
Karine Patrin Pontin ◽  
Juan D. Latorre ◽  
Mikayla F. A. Baxter ◽  
...  

Two experiments were conducted to evaluate the effect of the prophylactic or therapeutic administration of a 0.1% mixture containing ascorbic acid and a solid dispersion of curcumin with polyvinylpyrrolidone and boric acid (AA-CUR/PVP-BA) against Salmonella Enteritidis (S. Enteritidis) in broiler chickens. A third experiment was conducted to evaluate the impact of the dietary administration of 0.1% AA-CUR/PVP-BA in a necrotic enteritis (NE) model in broiler chickens. The prophylactic administration of 0.1% AA-CUR/PVP-BA significantly decreased S. Enteritidis colonization in cecal tonsils (CT) when compared to the positive control group (PC, p < 0.05). The therapeutic administration of 0.1% AA-CUR/PVP-BA significantly reduced the concentration of S. Enteritidis by 2.05 and 2.71 log in crop and CT, respectively, when compared with the PC on day 10 post-S. Enteritidis challenge. Furthermore, the serum FITC-d concentration and total intestinal IgA levels were also significantly lower in chickens that received 0.1% AA-CUR/PVP-BA. Contrary, the PC group showed significantly higher total intestinal IgA levels compared to the negative control or AA-CUR/PVP-BA groups in the NE model. However, 0.1% AA-CUR/PVP-BA showed a better effect in reducing the concentration of S. Enteritidis when compared to the NE model. Further studies with higher concentration of AA-CUR/PVP-BA into the feed to extend these preliminary results are currently being evaluated.


2020 ◽  
Vol 55 (3) ◽  
pp. 47-56
Author(s):  
I.R. Dolinin ◽  
◽  
G.V. Bazekin ◽  
E.N. Skovorodin ◽  
I.V. Chudov ◽  
...  

Poultry farming is one of the most important dynamically developing branches of the agro-industrial complex, providing the population with fullfledged food products. It is necessary to search for new useful biological substances to increase the efficiency of the industry. At the same time, biologically active substances must be not only highly effective but also harmless to animals and humans. To date, significant experimental data on the use of biological stimulants in poultry and animal husbandry have been accumulated. The research aimed to study the influence of the biological stimulator Nucleostim on the development of the thymus and its clinical and morphological structure. 100 ROSS 308 cross broiler chickens were used for the research. Experimental and control groups of animals were kept in the same conditions on a unique, balanced diet with free access to water. In the course of the research, it was found that in 3 experimental groups, the mass of internal organs of broiler chickens is greater than in the control group. The most significant increase in organs was observed in chickens receiving Nucleostim at a dose (10 g/kg of feed). The use of the biological stimulator Nucleostim as a top dressing in broiler chickens preserves the structure of the thymus, with clear visualization and differentiation of the cortical layer and brain matter, increases the mitotic activity of thymocytes, improves blood supply to the organ. As a result of research, it was found that Nucleostim has an immunomodulatory effect, which is expressed in hypertrophy and hyperplasia of the thymus, increasing the number of CD 68 macrophages, slowing down the thymus involution processes in broiler chickens.


Author(s):  
Р.В. КАЗАРЯН ◽  
А.С. БОРОДИХИН ◽  
А.Д. АЧМИЗ ◽  
М.В. ЛУКЬЯНЕНКО ◽  
П.В. МИРОШНИЧЕНКО

Проведена сравнительная оценка качества и пищевой ценности мяса цыплятбройлеров и яиц курнесушек, полученных от птиц опытной группы, в рацион которых добавляли в течение 6 и 4 недель соответственно кормовой концентрат, включающий комплекс биологически активных веществ и пробиотическую микрофлору, в количестве 0,3 к массе корма. В качестве контрольной была группа птиц, получавших только основной рацион. Установлено, что содержание белка и жира в мясе цыплятбройлеров опытной группы выше на 7,02 и 2,44 соответственно по сравнению с содержанием белка и жира в мясе цыплятбройлеров контрольной группы. Содержание фосфолипидов, витаминов А и Е в мясе цыплятбройлеров опытной группы соответственно в 2,12 2,4 и 2,2 раза выше по сравнению с аналогичным показателем контрольной группы. При исследовании кулинарных свойств мяса цыплятбройлеров установлено, что суммарная балльная оценка вареного фарша, приготовленного из мяса цыплятбройлеров опытной группы, составила 46,0 баллов, контрольной группы 40,5 баллов, бульона 39,8 и 38,0 баллов соответственно. Введение комплексного кормового концентрата в рацион молодняка курнесушек способствовало получению большего количества яиц (на 17,1) более высокой пищевой ценности по сравнению с аналогичными показателями контрольной группы. Установлено, что в яйцах, полученных от курнесушек опытной группы, содержится жира на 11,52, фосфолипидов в 2,08 раза, витаминов А и Е соответственно в 2,66 и 2,27 раза больше, чем в яйцах от курнесушек контрольной группы. Содержание вкаротина в желтке яиц от курнесушек опытной группы в 4,4 раза выше, чем в желтке яиц от курнесушек контрольной группы. Comparative evaluation of quality and nutritive value of meat of broiler chickens and egg laying hens, obtained from birds of the experimental group in which diet was added for 6 and 4 weeks respectively feed concentrate including biologically active substances and probiotic microflora in the amount of 0,3 by weight of the feed carried out. The group of birds obtained only the basic diet was as a control. It was found that the protein and fat content in the meat of broiler chickens of the experimental group is higher compared to the protein and fat content in the meat of broiler chickens of the control group by 7,02 and 2,44 respectively. The content of phospholipids, vitamins A and E in the meat of broiler chickens of the experimental group respectively in 2,12 2,4 and 2,2 times higher compared to the control group. In the study of culinary properties of meat of broiler chickens found that the total scoring of boiled minced meat prepared from meat of chickensbroilers experimental group amounted to 46,0 points, the control group is 40,5 points, broth 39,8 and 38,0 points respectively. The introduction of a complex feed concentrate in the diet of young laying hens contributes to the production of more eggs (on 17,1) of higher nutritional value compared to the control group. It was found that eggs obtained from laying hens of the experimental group contain fat by 11,52, phospholipids by 2,08 times, vitamins A and E respectively by 2,66 and 2,27 times more than eggs from laying hens of the control group. The content of вcarotene in the yolk of eggs from laying hens of the experimental group is 4,4 times higher than in the yolk of eggs from laying hens of the control group.


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