Association of Interleukin-1 and Inteleukin-1 Receptor Antagonist Gene Polymorphisms with Multiple Sclerosis in Azeri Population of Iran

2020 ◽  
Vol 20 (7) ◽  
pp. 1110-1116 ◽  
Author(s):  
Mohammad Asgharzadeh ◽  
Zahra A. Jigheh ◽  
Hossein S. Kafil ◽  
Mehdi Farhoudi ◽  
Daryoush S. Oskouei ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Gene Polymorphism ◽  
Fast Food ◽  
Inflammatory Responses ◽  
Interleukin 1 ◽  
Critical Role ◽  
Healthy Controls ◽  
Genotype 1 ◽  
Significant Difference ◽  
Dieting Behaviors

Background: Cytokines as important mediators have a critical role in appropriate immune responses, the irregular production of which can lead to Multiple Sclerosis (MS). Proinflammatory cytokine interleukin-1 (IL-1) triggers inflammatory responses. Function and production of the cytokine are influenced by IL-1 coding gene polymorphism and those antagonists gene polymorphism. Objective: The aim of the present study was to evaluate the possible correlation between MS and IL-1 related alleles in Azeri population of Iran. Methods: Variable number tandem repeats (VNTR) genotypes of 150 MS patients and 220 healthy non-relative controls were determined. Result: n the healthy controls, genotype TT at IL-1A (-889) location was significantly higher than the MS patients (p=0.0001). However, a significant difference was not found between the two groups in genotypic/allelic frequency at IL- 1B+ 3953 location. Evaluation of the IL-1RA gene revealed that genotype 1/2, and genotype 1/3 were significantly higher in the healthy controls and MS patients, respectively. Our findings indicated that the consumption of fast-food in MS patients was significantly higher than controls (p= <0.05). Also, a considerable number of MS patients had inappropriate dieting behaviors such as not eating breakfast (p= 0.0001), and irregular eating habits (p= 0.0001). Conclusion: Polymorphisms of the IL-1B genes and common alleles of IL-1RA were not considered as risk factors for MS disease. However, genotype TT at IL-1A (-889) location and the rare allele of IL-1RA3 can be a potential risk factor for the disease. Furthermore, inappropriate dieting behaviors and consumption of fast-food can increase the risk of MS.

scholarly journals The Association Between Salt and Potassium Intake with Multiple Sclerosis

2019 ◽  
Vol 5 (1) ◽  
pp. 23-27
Author(s):  
Sarah Ghorbani ◽  
◽  
Hamidreza Hatamian ◽  
Amirhossain Mahmoudzadeh ◽  
Sina Raeisi ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Sodium Chloride ◽  
Critical Role ◽  
Interleukin 17 ◽  
Chloride Salt ◽  
Healthy Controls ◽  
Potassium Intake ◽  
Th 17 ◽  
Number Of Patients ◽  
Significant Difference

Background: The number of patients with Multiple Sclerosis (MS) is increasing in Iran. Studies have shown that high sodium chloride (salt) and low potassium intake are associated with the development of MS. High physiological salt concentrations can lead to the induction of Interleukin-17 (IL-17) accompanied by the excessive generation of helper T-17 cells (Th-17). This cytokine plays a critical role in the pathogenesis of autoimmune diseases. This is while potassium supplementation has a blocking effect on IL-17 production. Objectives: Because of the role of salt and potassium in Th 17 development, we hypothesized that sodium chloride (NaCl) would be higher and potassium (K) would be lower in MS patients than healthy controls. Therefore, we investigated the association between salt and potassium intake with MS in Isfahan City population, Iran. Materials & Methods: A case-control study containing 23 patients and 23 healthy controls was performed in Isfahan City, Iran, 2016. NaCl and K levels were measured in 24-h urine. Using the Chi-square test, the patients’ laboratory values were compared with the healthy controls. The level of significance was set at P<0.05 in all analyses. All calculations were performed in SPSS, version 23.0. Results: In this study, urine Na levels were somewhat higher in cases than in controls (Interquartile Range [IQR]; 160[140-211] mEq/24 h vs. 128[83-166] mEq/24 h]) (P=0.027). These results show a significant relationship between urine-Na and MS. Urine k concentrations were lower in cases than controls [IQR; 47(27-70) mEq/24 h vs. 50(29-56) mEq/24 h] but we did not find a significant difference between two groups (P=0.807). Conclusion: Based on this study, a high level of sodium intake may be associated with MS; however, we did not find a significant difference between patients and controls with regard to potassium level.

scholarly journals Multiple Sclerosis and Diabetes Mellitus: Further Evidence of a Relationship

1982 ◽  
Vol 9 (4) ◽  
pp. 415-419 ◽  
Author(s):  
Sharon A. Warren ◽  
K.G. Warren
Keyword(s):  
Diabetes Mellitus ◽  
Multiple Sclerosis ◽  
Healthy Controls ◽  
Degree Relative ◽  
Significant Difference ◽  
The Difference ◽  
The Relationship ◽  
Prevalence Of Diabetes Mellitus ◽  
Second Degree

SUMMARY:One hundred multiple sclerosis (MS) patients were compared to healthy controls to determine the prevalence of diabetes mellitus in their families. Significantly, more MS patients than controls were diabetic or reported at least one first degree relative (parent, sibling, child) with diabetes. The relationship between MS and diabetes persisted when second degree relatives (grandparents, aunts and uncles) were taken into consideration.A greater percentage of MS patients with another MS relative were diabetic or reported a first degree relative with diabetes mellitus than MS patients without an MS relative. However the difference was not statistically significant. Nor was there a significant difference when percentages reporting either a first or a second degree relative with diabetes were compared.

Association of Thr715Pro P-Selectin Gene Polymorphism in Diabetic Retinopathy in North Indian Population: A Prospective Clinical Study

2021 ◽  
Vol 13 (2) ◽  
pp. 145-153
Author(s):  
Ritika Sharma ◽  
Shri Kant ◽  
Deepak Mishra ◽  
Tanmay Srivastav ◽  
Hemendra Singh
Keyword(s):  
Diabetic Retinopathy ◽  
Clinical Study ◽  
Gene Polymorphism ◽  
Indian Population ◽  
Prospective Clinical Study ◽  
Healthy Controls ◽  
Independent Factor ◽  
North Indian Population ◽  
Genotypic Analysis ◽  
Significant Difference

Introduction: The aim of this study was to evaluate the role of Thr715Pro P-Selectin gene polymorphism in patients with Diabetic Retinopathy in North Indian population and establish its role in the pathophysiology as an independent factor. Materials and methods: This is a prospective clinical study conducted on 60 patients at a tertiary care centre in North India over a period of eighteen months. Sixty patients satisfying the inclusion criteria were selected from the Vitreoretina clinic in the department. They were categorised equally in three groups namely Diabetics with diabetic retinopathy (DwDR), Diabetics without diabetic retinopathy (DwoDR), and non diabetics. The non-diabetics group was further divided into healthy controls, Hypertensive Retinopathy (HR) and Non-exudative Age Related Macular Degeneration (NEAMD). All the patients underwent complete ophthalmic evaluation and blood samples were drawn for the genetic study with their informed consent. Data was analysed using SPSS software version 16. Results: The genotypic analysis between DwDR, DwoDR and the three subgroup of controls comprising of healthy controls, HR and NEAMD showed that Thr715Pro (A/C) polymorphism prevalence was significantly high in DwDR (p = 0.003) and DwoDR (p = 0.003) compared to healthy controls. No significant difference was noted between DwDR, DwoDR and the HR and NEAMD groups.  Conclusion: Thr715Pro P-Selectin gene Polymorphism could not be established as an independent factor in the pathogenesis of diabetic retinopathy, as its association is found with other systemic diseases which create a prothrombotic state.

Interleukin-1 receptor antagonist gene polymorphism and multiple sclerosis

The Lancet ◽  
1995 ◽  
Vol 346 (8980) ◽  
pp. 979-980 ◽  
Author(s):  
J.B.A Crusius ◽  
A.S Peña ◽  
B.W van Oosten ◽  
G Bioque ◽  
A Garcia ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Gene Polymorphism ◽  
Receptor Antagonist ◽  
Interleukin 1 ◽  
Interleukin 1 Receptor Antagonist

scholarly journals Quantification of human plasma metalloproteins in multiple sclerosis, ischemic stroke and healthy controls reveals an association of haptoglobin-hemoglobin complexes with age

PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0262160
Author(s):  
Sophia Sarpong-Kumankomah ◽  
Katherine B. Knox ◽  
Michael E. Kelly ◽  
Gary Hunter ◽  
Bogdan Popescu ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Ischemic Stroke ◽  
Inductively Coupled Plasma ◽  
Rank Correlation ◽  
Positive Association ◽  
Disease Diagnosis ◽  
Size Exclusion ◽  
Control Group ◽  
Healthy Controls ◽  
Significant Difference

Advanced analytical methods play an important role in quantifying serum disease biomarkers. The problem of separating thousands of proteins can be reduced by analyzing for a ‘sub-proteome’, such as the ‘metalloproteome’, defined as all proteins that contain bound metals. We employed size exclusion chromatography (SEC) coupled to an inductively coupled plasma atomic emission spectrometer (ICP-AES) to analyze plasma from multiple sclerosis (MS) participants (n = 21), acute ischemic stroke (AIS) participants (n = 17) and healthy controls (n = 21) for Fe, Cu and Zn-metalloproteins. Using ANOVA analysis to compare the mean peak areas among the groups revealed no statistically significant differences for ceruloplasmin (p = 0.31), α2macroglobulin (p = 0.51) and transferrin (p = 0.31). However, a statistically significant difference was observed for the haptoglobin-hemoglobin (Hp-Hb) complex (p = 0.04), being driven by the difference between the control group and AIS (p = 0.012), but not with the MS group (p = 0.13), based on Dunnes test. A linear regression model for Hp-Hb complex with the groups now adjusted for age found no statistically significant differences between the groups (p = 0.95), but was suggestive for age (p = 0.057). To measure the strength of association between the Hp-Hb complex and age without possible modifications due to disease, we calculated the Spearman rank correlation in the healthy controls. The latter revealed a positive association (r = 0.39, 95% Confidence Interval = (-0.05, 0.83), which suggests that either the removal of Hp-Hb complexes from the blood circulation slows with age or that the release of Hb from red blood cells increases with age. We also observed that the Fe-peak corresponding to the Hp-Hb complex eluted ~100 s later in ~14% of all study samples, which was not correlated with age or disease diagnosis, but is consistent with the presence of the smaller Hp (1–1) isoform in 15% of the population.

scholarly journals A Randomized Control Trial Study to Determine the Effect of Melatonin on Serum Levels of IL-1β and TNF-α in Patients with Multiple Sclerosis

2020 ◽  
Author(s):  
Masoomeh Yosefifard ◽  
Gholamhassan Vaezi ◽  
Ali Akbar Malekirad ◽  
Fardin Faraji ◽  
Vida Hojati
Keyword(s):  
Multiple Sclerosis ◽  
Interleukin 1 ◽  
Serum Levels ◽  
Inflammatory Factors ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Treatment Groups ◽  
Tnf Α ◽  
Significant Difference ◽  
The Central Nervous System

Multiple sclerosis (MS) is the most common neurological disease that happens at a young age. MS is an inflammatory disease; associated with the demyelination of the central nervous system. Therefore, some inflammatory factors are effective in the mechanism and progression of the disease. Melatonin, as a multi-effect substance including anti-inflammatory effects, can reduce symptoms of MS in patients with a change in their inflammatory factors level. In this study, 50 MS patients who were referred to the MS Society of Markazi Province were randomly selected. All patients were treated with routine MS treatment (interferon) and were divided into control (25 placebo recipients) and treatment (25 recipients of 3 mg melatonin per day for 24 weeks) groups. Anthropometric data of patients including height, weight, and age were determined. Blood samples were collected after fasting in order to determine serum levels of interleukin 1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α). Then, samples were immediately centrifuged for serum separation and sera were transferred to a freezer at -80°C and serum levels of these factors were determined; using ELISA kit. The results of this study showed that there was no significant difference between the control and treatment groups in terms of serum levels of TNF-α. However, the level of IL-1β was significantly reduced in the treatment group compared to the control group, indicating that melatonin decreases this inflammatory substance. Our findings suggest a valuable strategy in the treatment of patients who suffer from MS

scholarly journals Investigation of the Correlation between Graves’ Ophthalmopathy and CTLA4 Gene Polymorphism

2019 ◽  
Vol 8 (11) ◽  
pp. 1842 ◽  
Author(s):  
Ding-Ping Chen ◽  
Yen-Chang Chu ◽  
Ying-Hao Wen ◽  
Wei-Tzu Lin ◽  
Ai-Ling Hour ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Regulatory Gene ◽  
Genotype Frequency ◽  
Healthy Controls ◽  
Nucleotide Polymorphisms ◽  
Exon 2 ◽  
Graves Ophthalmopathy ◽  
Potential Marker ◽  
Significant Difference ◽  
Ctla4 Gene

Graves’ disease (GD) is an autoimmune inflammatory disease, and Graves’ ophthalmopathy (GO) occurs in 25–50% of patients with GD. Several susceptible genes were identified to be associated with GO in some genetic analysis studies, including the immune regulatory gene CTLA4. We aimed to find out the correlation of CTLA4 gene polymorphism and GO. A total of 42 participants were enrolled in this study, consisting of 22 patients with GO and 20 healthy controls. Chi-square or Fisher’s exact test were used to appraise the association between Graves’ ophthalmopathy and CTLA4 single nucleotide polymorphisms (SNPs). All regions of CTLA4 including promoter, exon and 3’UTR were investigated. There was no nucleotide substitution in exon 2 and exon 3 of CTLA4 region, and the allele frequencies of CTLA4 polymorphisms had no significant difference between patients with GO and controls. However, the genotype frequency of “TT” genotype in rs733618 significantly differed between patients with GO and healthy controls (OR = 0.421, 95%CI: 0.290–0.611, p = 0.043), and the “CC” and “CT” genotype in rs16840252 were nearly significantly differed in genotype frequency (p = 0.052). Haplotype analysis showed that CTLA4 Crs733618Crs16840252 might increase the risk of GO (OR = 2.375, 95%CI: 1.636–3.448, p = 0.043). In conclusion, CTLA4 Crs733618Crs16840252 was found to be a potential marker for GO, and these haplotypes would be ethnicity-specific. Clinical application of CTLA4 Crs733618Crs16840252 in predicting GO in GD patients may be beneficial.

scholarly journals Kallikrein-related peptidase 6 exacerbates disease in an autoimmune model of multiple sclerosis

2016 ◽  
Vol 397 (12) ◽  
pp. 1277-1286 ◽  
Author(s):  
Hyesook Yoon ◽  
Isobel A. Scarisbrick
Keyword(s):  
Multiple Sclerosis ◽  
Spinal Cord ◽  
Inflammatory Cytokines ◽  
Immune Cells ◽  
Inflammatory Responses ◽  
Critical Role ◽  
Systemic Administration ◽  
Neurological Deficits ◽  
Interleukin 17 ◽  
The Impact

Abstract Kallikrein-related peptidase 6 (Klk6) is elevated in the serum of multiple sclerosis (MS) patients and is hypothesized to participate in inflammatory and neuropathogenic aspects of the disease. To test this hypothesis, we investigated the impact of systemic administration of recombinant Klk6 on the development and progression of MOG35-55-induced experimental autoimmune encephalomyelitis (EAE). First, we determined that Klk6 expression is elevated in the spinal cord of mice with EAE at the peak of clinical disease and in immune cells upon priming with the disease-initiating peptide in vitro. Systemic administration of recombinant Klk6 to mice during the priming phase of disease resulted in an exacerbation of clinical symptoms, including earlier onset of disease and higher levels of spinal cord inflammation and pathology. Treatment of MOG35-55-primed immune cells with Klk6 in culture enhanced expression of pro-inflammatory cytokines, interferon-γ, tumor necrosis factor, and interleukin-17, while reducing anti-inflammatory cytokines interleukin-4 and interleukin-5. Together these findings provide evidence that elevations in systemic Klk6 can bias the immune system towards pro-inflammatory responses capable of exacerbating the development of neuroinflammation and paralytic neurological deficits. We suggest that Klk6 represents an important target for conditions in which pro-inflammatory responses play a critical role in disease development, including MS.

Expression of Interleukin 1, Interleukin 27 and TNF α Genes in Patients with Ischemic Cardiomyopathy Versus Idiopathic Dilated Cardiomyopathy

2021 ◽  
Author(s):  
Mahdiyar Iravani Saadi ◽  
Javad Salami ◽  
Hanieh Abdi ◽  
Ehsan Nabi Abdolyousefi ◽  
Ahmad Niknam ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dilated Cardiomyopathy ◽  
Inflammatory Cytokines ◽  
Interleukin 1 ◽  
Expression Level ◽  
Idiopathic Dilated Cardiomyopathy ◽  
Healthy Controls ◽  
Idiopathic Cardiomyopathy ◽  
Tnf Α ◽  
Significant Difference

Abstract Objective: Congestive Heart failure (CHF) is a complex multifactorial syndrome due to tissue hypo perfusion that is affected by some factors like inflammatory cytokines. In our study we investigated the exact gene expression of three inflammatory cytokines in ischemic and idiopathic cardiomyopathy patients.Materials and Methods: From 49 studied recipients in ischemic group, 23 (46.9%) were male and from 40 studied recipients in idiopathic dilated cardiomyopathy group, 19 (47.5%) were male. For the quantitative analysis of Interleukin (IL)1, IL-27 and TNF-α mRNAs expression level, the SYBR Green Real-Time PCR method was performed using SYBRPremix Ex TaqTM II (Tli RNaseH Plus) (Takara, Japan) and designed primers specific for each genes in an iQ5 thermocycler (BioRad Laboratories, USA) according to the manufacturer’s instructions.Results: Our results showed that the expression level of IL-1 and TNF-α were significantly higher in the ischemic patients compared to healthy controls (P<0.001, P<0.01, respectively); also we found higher levels of IL-1 and IL-27 gene expressions in idiopathic patients compared to healthy controls (P<0.001, P<0.001, respectively). There were not any significant difference of IL-1, IL-27 and TNF-α expression levels between ischemic patients and idiopathic ones.Conclusion: Although we would introduce IL1, IL-27 and TNF α as effective inflammatory cytokines on myocardial functions in ischemic and idiopathic cardiomyopathy patients; there is not any difference between these two groups in gene expression of three main inflammatory cytokines.

Vitamin D Receptor Gene Polymorphism and the Risk of Multiple Sclerosis in Azeri Population of Iran

2020 ◽  
Vol 20 ◽  
Author(s):  
Mahya Pourostadi ◽  
Simin Sattarpour ◽  
Behroz Mahdavi Poor ◽  
Mohammad Asgharzadeh ◽  
Hossein Samadi Kafil ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Vitamin D ◽  
Vitamin D Receptor ◽  
Receptor Gene ◽  
Red Meat ◽  
Healthy Controls ◽  
Vdr Gene ◽  
Pcr Rflp ◽  
Excessive Consumption ◽  
Dieting Behaviors

Background: Multiple sclerosis (MS), a disease of central nervous system (CNS), is associated with damage to the myelin sheath of neurons. It is demonstrated that vitamin D deficiency plays an important role in the development of the disease. Binding of vitamin D to its specific nuclear receptors is way to exert its function. Objective: Possible correlation between vitamin D receptor (VDR) gene and MS was evaluated in Azeri population of Iran. Method: Different genotypes of Bsml site were determined by using PCR-RFLP method in 148 MS patients and 220 nonrelative healthy controls. Results: In MS patients, genotype bb was significantly higher than the healthy controls (p<0.05). Additionally, most subjects of MS group had been insufficiently exposed to sunlight before the age of 15 (p<0.001). Our findings indicated that the red meat intake in MS patients was significantly higher than the healthy controls (p<0.001). In addition, the healthy controls had appropriate dieting behaviors in comparison to MS patients (excessive intake of some foods) (p=0.0001). Conclusions: In conclusion, genotype BB and sufficient exposure to sunlight before the age of 15 were the protective factors against MS. Although, excessive consumption of red meat and inappropriate dieting behaviors were predisposing factors to MS disease.

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