First reporting of Anisakis sp. in the Armed Snook fish (Centropomus armatus) caught and commercialized in Buenaventura, Colombia

Infectio ◽  
2018 ◽  
Vol 22 (3) ◽  
pp. 136
Author(s):  
Jenniffer Alejandra Castellanos ◽  
Andrés Ricardo Tangua ◽  
Rubén Mercado ◽  
Liliana Salazar
Keyword(s):  
Health Personnel ◽  
Aquatic Organisms ◽  
Human Consumption ◽  
Morphological Identification ◽  
Pacific Region ◽  
Port City ◽  
Artisanal Fishing ◽  
The Mean ◽  
Hematoxylin Eosin ◽  
Anisakid Nematodes

Objective: Nematodes of the Anisakidae family are parasites found in aquatic organisms. The lack of studies on anisakidosis and Anisakis in Colombia has meant this type of parasitosis is not widely known by health personnel and underreporting of the disease is highly likely. The objective of this study was to identify anisakid nematodes in the armed snook fish (Centropomus armatus) obtained by artisanal fishing and sold commercially in the coastal port city of Buenaventura.Material and methods: Morphological identification of these worms was performed using taxonomic keys and supplemented with microscopic study using the histochemical Hematoxylin-Eosin technique.Results: Nematodes of the genus Anisakis were found in 42% and the mean abundance was 2.8 in the C. armatus.Conclusions: The findings confirm the presence of Anisakis sp. in fish for human consumption in Buenaventura, the main fishing port in the Colombian Pacific region. This finding in itself warrants further investigation into the possibility of an emerging disease in Colombia.

Vesseg: An Open-Source Tool for Deep Learning-Based Atherosclerotic Plaque Quantification in Histopathology Image

2021 ◽  
Author(s):  
J.M. Murray ◽  
P. Pfeffer ◽  
R. Seifert ◽  
A. Hermann ◽  
J. Handke ◽  
...  
Keyword(s):  
Deep Learning ◽  
Open Source ◽  
Atherosclerotic Plaque ◽  
State Of The Art ◽  
The Mean ◽  
Time Savings ◽  
Plaque Quantification ◽  
Atherosclerosis Research ◽  
Hematoxylin Eosin ◽  
Microscopy Images

Objective: Manual plaque segmentation in microscopy images is a time-consuming process in atherosclerosis research and potentially subject to unacceptable user-to-user variability and observer bias. We address this by releasing Vesseg a tool that includes state-of-the-art deep learning models for atherosclerotic plaque segmentation. Approach and Results: Vesseg is a containerized, extensible, open-source, and user-oriented tool. It includes 2 models, trained and tested on 1089 hematoxylin-eosin stained mouse model atherosclerotic brachiocephalic artery sections. The models were compared to 3 human raters. Vesseg can be accessed at https://vesseg .online or downloaded. The models show mean Soerensen-Dice scores of 0.91±0.15 for plaque and 0.97±0.08 for lumen pixels. The mean accuracy is 0.98±0.05. Vesseg is already in active use, generating time savings of >10 minutes per slide. Conclusions: Vesseg brings state-of-the-art deep learning methods to atherosclerosis research, providing drastic time savings, while allowing for continuous improvement of models and the underlying pipeline.

Evaluation of heavy metals content in the canned/packed fruit juices from local and imported origin in Lahore, Pakistan

2021 ◽  
Author(s):  
Nazeefa Fatima ◽  
Munazza Khan ◽  
Muhammad Shuaib Kabeer
Keyword(s):  
Heavy Metals ◽  
Atomic Absorption ◽  
Fruit Juices ◽  
Human Consumption ◽  
Acid Digestion ◽  
Digestion Method ◽  
Mean Values ◽  
Nutritive Values ◽  
The Mean ◽  
Mean Concentration

This study was conducted to determine the mean concentration of heavy metals such as lead (Pb), copper (Cu), zinc (Zn), chromium (Cr), manganese (Mn), nickel (Ni), selenium (Se), magnesium (Mg), and iron (Fe) in canned/packed fruits juices, collected from various stores in Lahore in a period of three months. These juices were categorized into four groups; local packed and canned and also imported packed and canned products. Every group consisted of ten samples. By using the di-acid digestion method, the collected samples were digested and analyzed under Atomic Absorption Spectrophotometer (AAS). The results indicated that the mean values of 7 out of 9 tested heavy metals including Pb, Mg, Ni, Fe, Cr, Se and Mn were above permissible limits (set by WHO) in all four understudy groups. Therefore, it was concluded that commercially available fruit juices are not all safe according to their heavy metals content for the human consumption despite their nutritive values.

PHYSICO-CHEMICAL AND SOME HEAVY METALS CONCENTRATION ANALYSES ON THREE COMMON SPECIES OF FISH IN TUNGAN KAWO RESERVOIR KONTAGORA, NIGER STATE, NIGERIA

2021 ◽  
Vol 5 (1) ◽  
pp. 223-232
Author(s):  
Mohammed Jr. Kinta ◽  
A. V. Ayanwale ◽  
U. N. Keke ◽  
Y. I. Auta ◽  
B. S. Adama ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Drinking Water ◽  
Oxygen Demand ◽  
Aquatic Organisms ◽  
Common Species ◽  
Human Consumption ◽  
Long Term Effects ◽  
Aquatic Life ◽  
Physico Chemical ◽  
Fish Samples

Developing countries like Nigeria are faced with increased in generation of domestic, industrial and agricultural wastes, with a large percentage moving. This study evaluates the physico-chemical and some heavy metals concentration in three common species of fish from Tungan Kawo reservoir Kontagora, Nigeria; using standard methods between (July 2018 – February 2019); at four different sampling stations of human activities on the water. Five heavy metals were evaluated (Lead, Copper, Manganese, Iron and Chromium) in the fish samples. Phosphate (0.4 – 2.5) mg/L, Nitrate (3.2 – 7.5) mg/L, Temperature (27 – 32.4) 0C, Dissolved Oxygen (2.4 – 5.2 mg/L), Conductivity (81 – 125 µS/cm), Biochemical Oxygen Demand (1.9 – 4.4 mg/L), Alkalinity (mg/L) and Total Dissolved Solids (117 – 198) ppm were within the standard for drinking water and survival of fish.  However, the pH (6.3 – 9.8) was above the standard for NIS and WHO drinking water but can support aquatic life. Iron (0.64 ± 0.072 mg/kg) was the most highly concentrated in Synodontis clarias while lead (0.01 ± 0.013 mg/kg) was the lowest in Oreochromis niloticus and Coptidon zillii (formerly Tilapia zillii. This current finding indicates that the water is safe for both aquatic life and domestic purpose but not suitable for direct human consumption without being properly treated. However, there is the need for regular monitoring of the heavy metals load in this water body and the aquatic organisms because of the long term effects

scholarly journals Quality assessment of Zeus faber (Peter’s fish) ovaries regularly commercialized for human consumption

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Filippo Giarratana ◽  
Graziella Ziino ◽  
Valerio D'Andrea ◽  
Antonio Panebianco ◽  
Alessandro Giuffrida
Keyword(s):  
Quality Assessment ◽  
Mean Value ◽  
Raw Material ◽  
Human Consumption ◽  
Salmonella Spp ◽  
The Mean ◽  
Fresh Products ◽  
Vibrio Spp ◽  
Zeus Faber

n the last few years, the consumption of fish eggs has increased rapidly, finding widespread use also in mass catering. This increase has involved also those of the Peter’s fish (Zeus faber). Females of this species, by their reproductive characteristics, have highly developed gonads in different periods of the year, making the raw material easy to find. The aim of the present study was to perform a quality assessment of Zeus faber ovaries regularly commercialized for human consumption. A total number of 34 samples, divided in fresh (11) and frozen (23), were processed for microbiological characterization, parasitological and histological evaluations. Fresh and frozen samples have significant (P<0.01) differences in total bacterial charge, with values of 4.75±0.5 Log CFU/g and 3.65±0.7 Log CFU/g respectively. The mean value of Enterobacteriaceae was 2.58±0.7 Log CFU/g in fresh products, while 52.17% (12) of frozen samples reported loads of <1 Log CFU/g. No Salmonella spp. and Listeria monocytogenes were found. Aeromonas spp. was detected in two frozen sample (with loads of 2.2 and <1 Log CFU/g) and in 5 fresh ovaries with value ranged from 1.70 to 3.48 Log CFU/g. Vibrio spp. was found in 4 (36.36%) and 3 (13.04%) of fresh and frozen samples respectively, with loads always <1 Log CFU/g. All 31 Vibrio strains isolated, were identified as Vibrio alginolyticus, and 61.29% (19) of them was positive for the ToxRS factor and 6.45% (2) for ToxR. The 47.06% (16) of total samples showed infestations by larvae of Anisakis Type 1 in the serous and inside the ovary. In this last case, histologically it was found to be free larvae. This study attested satisfactory hygiene conditions for Zeus faber ovaries currently marked for human consumption. The presence of potentially pathogenic strains of V. alginolyticus and Aeromonas spp., but above all the frequent infestation by Anisakis larvae, represent a potentially hazard for the consumer.

scholarly journals Relative salinity tolerance of freshwater macroinvertebrates from the south-east Eastern Cape, South Africa compared with the Barwon Catchment, Victoria, Australia

2005 ◽  
Vol 56 (2) ◽  
pp. 163 ◽  
Author(s):  
Ben J. Kefford ◽  
Carolyn G. Palmer ◽  
Dayanthi Nugegoda
Keyword(s):  
South Africa ◽  
Aquatic Organisms ◽  
Sea Salt ◽  
Eastern Cape ◽  
Median Lethal Concentration ◽  
Species Sensitivity ◽  
Freshwater Macroinvertebrates ◽  
The South ◽  
Sensitivity Distribution ◽  
The Mean

Salinity is rising in many southern African and Australian rivers with unknown effects on aquatic organisms. The extent of spatial variation, at any scale, in salt tolerances of aquatic organisms is unknown, so whether data from one location is applicable elsewhere is also unknown. The acute tolerances (72-h median lethal concentration (LC50)) to sea salt of 49 macroinvertebrate taxa from the south-east Eastern Cape (SEEC), South Africa were compared with those of 57 species from the Barwon Catchment, Victoria, Australia. The mean LC50 values from both locations were similar (Barwon: 31 and SEEC: 32 mS cm−1) and less abundant (rare) taxa tended to be more tolerant than more abundant (common) taxa. There was, however, a greater range of LC50 values (5.5–76 mS cm−1) in the Barwon Catchment than in the SEEC (11–47 mS cm−1). The species sensitivity distribution (SSD) for SEEC taxa was bimodal whereas the Barwon Catchment’s SSD had a single peak. With few exceptions, members of an order had similar tolerances in both locations. The differences in SSD between locations were related to crustacean, odonate and non-arthropod relative richness. Although it is not ideal to extrapolate SSDs from one location to another, it may be reasonable to assume similar salinity tolerances among related taxa.

scholarly journals Tangential Biopsy Thickness versus Lesion Depth in Longitudinal Melanonychia: A Pilot Study

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Nilton Di Chiacchio ◽  
Walter Refkalefsky Loureiro ◽  
Nilceo Schwery Michalany ◽  
Felipe Veiga Kezam Gabriel
Keyword(s):  
Nail Plate ◽  
Histopathological Diagnosis ◽  
Total Thickness ◽  
Thickness Measure ◽  
Histopathologic Diagnosis ◽  
The Matrix ◽  
General Average ◽  
The Mean ◽  
Longitudinal Melanonychia ◽  
Hematoxylin Eosin

Longitudinal melanonychia can be caused by melanocyte activation (hypermelanosis) or proliferation (lentigo, nevus or melanoma). Histopathologic examination is mandatory for suspicious cases of melanomas. Tangential biopsy of the matrix is an elegant technique avoiding nail plate dystrophy, but it was unknown whether the depth of the sample obtained by this method is adequate for histopathologic diagnosis. Twenty-two patients with longitudinal melanonychia striata were submitted to tangential matrix biopsies described by Haneke. The tissue was stained with hematoxylin-eosin and the specimens were measured at 3 distinct points according to the total thickness: largest (A), intermediate (B) and narrowest (C) then divided into 4 groups according to the histopathologic diagnosis (G1: hypermelanosis; G2: lentigos; G3: nevus; G4: melanoma). The lesions were measured using the same method. The mean specimen/lesion thickness measure values for each group was: G1: 0,59/0,10 mm, G2: 0,67/0,08 mm, G3: 0,52/0,05 mm, G4: 0,58/0,10 mm. The general average thickness for all the specimens/lesions was 0,59/0,08 mm. We concluded that the tangential excision, for longitudinal melanonychia, provides an adequate material for histopathological diagnosis.

scholarly journals Cadmium, Copper, Lead, and Zinc Contents of Fish Marketed in NW Mexico

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Martín G. Frías-Espericueta ◽  
Francia K. G. Zamora-Sarabia ◽  
J. Isidro Osuna-López ◽  
María D. Muy-Rangel ◽  
Werner Rubio-Carrasco ◽  
...  
Keyword(s):  
Dry Weight ◽  
Fish Muscle ◽  
Atomic Absorption Spectrophotometry ◽  
Human Consumption ◽  
Fish Muscles ◽  
Absorption Spectrophotometry ◽  
Nw Mexico ◽  
Lead And Zinc ◽  
The Mean ◽  
Copper Lead

To assess if they were within the safety limits for human consumption, the Cd, Cu, Pb, and Zn contents of fish muscles, bought from separate stalls of the fish markets of nine cities of NW Mexico, were determined by atomic absorption spectrophotometry. Considering all fish and markets, the mean contents were Zn:23.23±5.83, Cu:1.72±0.63, Cd:0.27±0.07, and Pb:0.09±0.04 µg/g (dry weight). Cu, Zn, and Pb did not reach levels of concern for human consumption, but the high Cd values determined in Mazatlán (Mugil cephalus:0.48±0.15;Diapterusspp.:0.57±0.33;Lutjanusspp.:0.72±0.12; small shark:0.87±0.19 µg/g dry weight) indicate that this was the only metal of concern for human health because the daily individual consumption of fish muscle to reach the PTDI would be within 0.27 and 0.41 kg.

Selenium concentrations in tissues of sheep given a subcutaneous injection of barium selenate or sodium selenate

1994 ◽  
Vol 34 (5) ◽  
pp. 581 ◽  
Author(s):  
JA Archer ◽  
GJ Judson
Keyword(s):  
Body Weight ◽  
Subcutaneous Injection ◽  
Mean Value ◽  
The Body ◽  
Transient Increase ◽  
Sodium Selenate ◽  
Human Consumption ◽  
Protective Mechanism ◽  
Treatment Groups ◽  
The Mean

Young sheep were allocated to 6 treatment groups, each of 8 ewes and 8 wethers. Treatments given were nil, 0.1, or 0.5 mg selenium (Se)/kg body weight as a subcutaneous injection of sodium selenate (Na2SeO4), and 0.8, 1.4, or 2.9 mg Se/kg body weight as a subcutaneous injection of barium selenate (BaSeO4). At 14, 28, 56, and 112 days after treatment, 2 ewes and 2 wethers from each group were slaughtered and samples of blood, liver, kidney, cardiac and skeletal muscle, lymph nodes, and faeces were collected for Se assay. On days 0, 1, 3, 7, 13, 27, and 55 blood and spot faecal samples were taken for Se assay from the 24 sheep selected for slaughter on day 112. In sheep given Na2Se04 there was a rapid but transient increase in the mean Se concentration in tissues, particularly the liver of sheep given the highest dose of Na2SeO4. In these sheep on days 14,28,56, and 112 the respective mean liver concentrations were 169, 62,25, and 6.9 �mol/kg DM: the mean value on day 14 was 15 times the mean value in untreated sheep. In sheep given BaSeO4 there was a gradual increase in mean Se concentration of tissues during the experiment. Faecal Se concentrations increased in sheep given the Se injections. The marked but transient increase in liver Se concentrations in sheep given Na2SeO4 suggests that this organ provides an important protective mechanism against toxicity by readily accumulating and excreting Se into the gut. Our results indicate that the BaSeO4 doses were unlikely to cause Se toxicity in young sheep or result in Se residues in tissues above those recommended for human consumption. The BaSeO4 should be administered subcutaneously in the neck or other sites of the body not sold for human consumption, since deposits of BaSeO4 remain at the site of injection for at least 112 days and may be dangerous if inadvertently consumed.

scholarly journals Fungi in bottled water

2018 ◽  
pp. 410-416
Author(s):  
Pollyana Rubem da Silva ◽  
Amanda Rafaela Carneiro de Mesquita ◽  
Marcos Antonio Barbosa de Lima ◽  
Edleide Freitas Pires
Keyword(s):  
Filamentous Fungi ◽  
Bottled Water ◽  
Human Consumption ◽  
Morphological Identification ◽  
Water Contaminants ◽  
Pet Bottles ◽  
Brazilian Legislation ◽  
Water And Wastewater ◽  
The City ◽  
Penicillium Spp

Bottled water is a product considered safe, although studies already proved fungi incidence even of potentially pathogens. The Brazilian legislation does not set limits for this microorganism in water for human consumption, limiting only for bacteria. This study evaluated the fungi contamination in bottled water commercialized in the region of the city of Recife-PE, Brazil. The samples were collected between september and december of 2016. Thirty five samples originated from seven companies, different lots, bottled in 500 mL polyethylene terephthalate (PET) bottles and coded from A to G and analyzed to fungi counting by mean of pour plate technique using acidified Potato Dextrose Agar (PDA) at pH 4.0 and incubated to 25°C±2°C for up to seven days, accordingly to the Standard Methods for the Examination of Water and Wastewater Methodology. pH data from the samples were obtained by information containing in the labels from the packages. It was observed the fungi presence in 20 samples (57.1%). From the positive samples, there were identified filamentous fungi in 14 (70%), only yeast in 1 (5%), and both in 5 (25%). Packages were considered contamination source. The predominant filamentous fungi in each sample were isolated, purified and submitted to morphological identification at the genus level. The results showed that Aspergillus spp. and Penicillium spp. can be water contaminants. The samples pH, around 5.0, might have contributed for the found fungi maintenance. The filamentous fungi found may be pathogenic or toxigenic, which represents a risk to public health.

scholarly journals Effects of plastics and microplastics on aquatic organisms and human health

2020 ◽  
Vol 37 (4) ◽  
pp. 437-443
Author(s):  
Fevziye Nihan Bulat ◽  
Berna Kılınç
Keyword(s):  
Environmental Pollution ◽  
Human Health ◽  
Water Environment ◽  
Aquatic Organisms ◽  
Human Consumption ◽  
Aquatic Species ◽  
Food Network ◽  
The Subject ◽  
Day By Day

Today, plastics have been used in many areas and their use have been increasing day by day. This increase in the use of plastic causes environmental pollution as well as negatively affects organisms in the environment and therefore human health. Plastics reach to the water environment through different transport routes. Microplastics that have reached to the water environment are consumed by aquatic organisms. Microplastics in aquatic species (fish, mussels, shrimp, seals, etc.) were highlighted in many studies. Microplastics consumed by aquatic organisms are included in the food network, reaching as far as human consumption. Therefore, the importance of the subject have been emphasized, the evaluation of the studies on microplastics and the risks it poses have been examined in this compilation study which was carried out in order to raise awareness about this issue and to ensure that the necessary measures will be taken.

Sign in / Sign up

Export Citation Format

Share Document