Effect of Different Extraction Method on Total Flavonoid Contents of Sansevieria trifasciata P. Leaves Extract

2021 ◽  
Vol 7 (2) ◽  
pp. 143-151
Author(s):  
Gina Septiani ◽  
Susanti Susanti ◽  
Fitria Sucitra
Keyword(s):  
Plant Extract ◽  
Extraction Method ◽  
Statistical Tests ◽  
Health Sector ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Total Flavonoid ◽  
Total Flavonoids ◽  
Amount Of Substance ◽  
Anti Inflammatory

Sanseviera leaves (Sansevieria trifasciata P.) is a plant that contains flavonoids. Flavonoid in the health sector act as antibacterial, antioxidant, anti inflammatory and anti diabetic. The extraction method will determine the amount of substance on the plant extract. The purpose of this study was to determine the effect of different extraction method on total flavonoid contents of sansevieria leaves extract. The method used is an experimental by comparing meceration and soxhletation extraction method to the total flavonoid contents of sansevieria leaves extract using UV-Vis spectrophotometric. The results showed that the total flavonoid contents of the maceration extraction method was 13.934 mgQE/g or 1.39% higher than the soxhlet extraction method of 8.117 mgQE/g or 0.81%. The results of statistical tests showed that  significant value of 0.001 (p<0.05),  means that there is a significant effect between the contents of total flavonoids in maceration and soxhlet extraction methods.

scholarly journals PENGARUH METODE EKSTRAKSI MINYAK PUPA ULAT JERMAN TERHADAP KOMPOSISI KIMIA

BUANA SAINS ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 67
Author(s):  
Erik Priyo Santoso ◽  
Farida Kusuma Astuti
Keyword(s):  
Extraction Method ◽  
Crude Protein ◽  
Dry Matter ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Chemistry Laboratory ◽  
Research Material ◽  
Soxhlet Method ◽  
Heat Method

The aim of this research is to know the extraction method of caterpillar pupa without detrimental to chemical composition to know the amount of pupa production of German caterpillar with two extraction methods per 100 grams of pupa To know the content of dry matter, organic matter, crude protein, crude fat, crude fiber and amino acid lysin, methionine, and vitamin E pupa oil of German caterpillar. The study was conducted from February to April 2017, at the Lab. Central University Tribhuwana Tunggadewi. Oil extraction analysis was conducted in the chemistry laboratory of Tribhuwana Tunggadewi University and chemistry laboratory of Poltek Malang. German caterpillar research material, German caterpillar pupa, pupa caterpillar germany extracted by maceration and sokletasi. German caterpillar pupa is obtained from German caterpillar breeders in Amadanom, Dampit, Malang Regency. This research uses two different extraction methods, namely cold extraction method (maseration method) and using heat method (soxhlet method). The conclusions of both research methods are known that the quantity of German caterpillar yield using soxhlet extraction method is more than the maceration extraction method. However, for the quality of the two methods it is still necessary to do further analysis to know the exact comparison of the two methods.

The Effects of Solvents and Extraction Methods on the Antioxidant Activity of P. niruri

2014 ◽  
Vol 68 (5) ◽  
Author(s):  
Hafizah Mohd Hadzri ◽  
Mohd Azizi Che Yunus ◽  
Salman Zhari ◽  
Fahim Rithwan
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Soxhlet Extraction ◽  
Solvent Polarity ◽  
Extraction Methods ◽  
The Other ◽  
Natural Antioxidant ◽  
Total Phenolic

The effects of different types of solvents and extraction method were investigated to determine the presence of antioxidant contents and activity from the P. niruri plant. The aim of this study is to determine which extraction method will give higher natural antioxidant contents and antioxidant activity. The content of natural antioxidant and antioxidant activity were analysed by total phenolic content (TPC), total flavonoid content (TFC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. The results showed that extracts from a supercritical fluid extraction (SFE) method without the addition of modifier showed the highest content of total phenolic (187.66 mg GAE/ g) and flavonoid (1100.93 mg QE/g) in P. niruri compared to the other methods of extraction with different type of solvents. The extract of P. niruri from different extraction methods showed antioxidant activity on DPPH radical scavenging assay. The soxhlet extraction method by methanol showed the lowest IC50 compared to the other methods of extraction. The results revealed that P. niruri extracts had different content of antioxidant and antioxidant activity. The solvent polarity and different methods of extraction play significant roles in determining the most suitable method for production of antioxidant contents and antioxidant activity from P. niruri extracts.

Comparison of Different Extraction Methods for Antioxidant Property of Flavonoids from Pomelo Peel

2013 ◽  
Vol 652-654 ◽  
pp. 443-448 ◽  
Author(s):  
Qiao Mei Ru ◽  
Ru Fan Cai ◽  
Jin Zhe He
Keyword(s):  
Extraction Method ◽  
Antioxidant Activities ◽  
Soxhlet Extraction ◽  
Antioxidant Property ◽  
Extraction Methods ◽  
Antioxidant Ability ◽  
Microwave Assisted ◽  
Pomelo Peel ◽  
Assisted Extraction ◽  
Different Levels

Antioxidant activity of the flavonoids from pomelo (Citrus grandis (L.) Osbeck) peel obtained by conventional soxhlet extraction (CSE), microwave-assisted extraction (MAE) and supercritical carbon dioxide (SC-CO2) extraction were investigated. For measuring antioxidant activities, different methods have been used corresponding to different levels of antioxidant action. The results indicated that the extraction yields of flavonoids by MAE and SC-CO2 extraction were higher than that by CSE method, whereas flavoniods by SC-CO2 extraction exhibited the strongest antioxidant activities. Our results indicated that SC-CO2 extraction method could effectively reserve the antioxidant ability of pomelo peel flavonoids.

scholarly journals Valorisation of Olea europaea L. Olive Leaves through the Evaluation of Their Extracts: Antioxidant and Antimicrobial Activity

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 966
Author(s):  
Mónica Sánchez-Gutiérrez ◽  
Isabel Bascón-Villegas ◽  
Alejandro Rodríguez ◽  
Fernando Pérez-Rodríguez ◽  
África Fernández-Prior ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Olea Europaea ◽  
Extraction Method ◽  
Water Extract ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Total Phenol Content ◽  
Olive Leaves ◽  
Olea Europaea L ◽  
Olea Europaéa

Olea europaea L. leaves constitute a source of bioactive compounds with recognized benefits for both human health and technological purposes. In the present work, different extracts from olive leaves were obtained by the application of two extraction methods, Soxhlet and microwave-assisted extraction (MAE), and six solvents (distilled water, ethanolic and glycerol mixtures solvents). MAE was applied under 40, 60 and 80 °C for 3, 6.5 and 10 min. The effect of the extraction method, solvent and treatment factors (the latter in MAE) on the total phenol content (TPC), the antioxidant activity (AA) and the phenolic profile of the extracts were all evaluated. The extracts showed high values of TPC (up to 76.1 mg GAE/g DW) and AA (up to 78 mg TE/g DW), with oleuropein being the most predominant compound in all extracts. The Soxhlet extraction method exhibited better yields in TPC than in MAE, although both methods presented comparable AA values. The water MAE extract presented the strongest antimicrobial activity against five foodborne pathogens, with minimum inhibitory concentration (MIC) values ranging from 2.5 to 60 mg/mL. MAE water extract is proposed to be exploited in the food and nutraceutical industry in the frame of a sustainable economy.

scholarly journals Analysis of Secondary Metabolites from Infloroscense Extract of Achyranthus Aspera

2021 ◽  
Vol 9 (10) ◽  
pp. 297-299
Author(s):  
Keerti Samdariya
Keyword(s):  
Free Radicals ◽  
Secondary Metabolites ◽  
Qualitative Study ◽  
Plant Extract ◽  
Extraction Method ◽  
Active Component ◽  
Soxhlet Extraction ◽  
Antioxidant Defence ◽  
Antiallergic Activity ◽  
Antioxidant Defence System

Abstract: The qualitative study of extract of AchyranthesAspera influoroscence shows the presence of different secondary metabolites. The study of active component of influoroscence of Achyranthesaspera was extracted . Plant extract contains several secondary metabolites .The extract was used for different phytochemical test. Test was performed using extract prepared by soxhlet extraction method. This plant have anti oxidative agent ,free radicals , phytoconstituents , and carcinogen detoxification and antioxidant defence system. Keywords: secondary metabolites, phytochemical test, antiallergic activity, antiperoxidative agent ,detoxification etc.

scholarly journals Determination of Oil Content and Physicochemical Properties of Oil Extracted from Niger Seed Oil Grown in Gamo Gofa, Southern Ethiopia

2016 ◽  
Vol 63 ◽  
pp. 141-144
Author(s):  
Bezuayehu Getachew ◽  
Kemal Ahmed ◽  
Mahmud Endris ◽  
Manale Zebene ◽  
Tsegay Hiwot ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Extraction Method ◽  
Oil Content ◽  
Seed Oil ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Chemical Parameters ◽  
Physico Chemical ◽  
Gamo Gofa ◽  
Niger Seed

The Niger seed oil was collected from gamo-gofa, southern part of Ethiopia for oil extraction. The collected seed were oven dried and crushed in to powder by mortar and pestle. A soxhlet and maceration extractions were used for extraction of the oil. The solvents used for both extractions were n-hexane and ethyl acetate. The main reason two different types of solvents and two different type of extraction methods used in this project were to check which type of solvent and extraction method were effective for extraction of oil from Niger seed. From both extraction methods the extracted oil was separated from the solvents by simple distillation. The oil content and the physico-chemical parameters of the oil were determined and an oil content of 23.45% and 21.35% were obtained by maceration and 33.02% and 22.7% by soxhlet extraction method from n-hexane and ethyl acetate respectively. The physico-chemical parameters of the seed and oil were determined and the result shows that moisture content of the seed was (8.3%) and acid value of the oil (1.7391), saponification value (6.0308), Kinematic viscosity (0.561 m2/s), Density of oil (0.9788 g/ml) and Specific gravity of (0.9947). From the result obtained we conclude that n-hexane is an effective solvent and soxhlet extraction is an effective extraction method for extraction of oil from Niger seed.

scholarly journals Impacts of Extraction Methods and Solvent Systems in the Assessment of Toxic Organic Compounds in a Solid Matrix

2016 ◽  
Vol 1 (1) ◽  
pp. 23 ◽  
Author(s):  
K. E. Ugwu ◽  
P. O. Ukoha
Keyword(s):  
Organic Compounds ◽  
Extraction Method ◽  
Environmental Concern ◽  
Soxhlet Extraction ◽  
Solvent System ◽  
Extraction Methods ◽  
Solid Matrix ◽  
Binary Solvent ◽  
Solvent Mixtures ◽  
Solvent Systems

Polycyclic Aromatic Hydrocarbons (PAHs) are among the listed persistent organic compounds (POP) which are pollutants of environmental concern due to their toxicity. This study evaluated soxhlet and ultrasonic extraction methods using a three-solvent system (acetone+dichloromethane+n-hexane) in order to compare the ability of the techniques to extract selected PAHs in raw coals collected from a coal mine in Okobo-Enjema, Nigeria. Then, binary solvent mixtures consisting of acetone+dichloromethane; dichloromethane+n-hexane; and acetone+n-hexane, were compared with the ternary solvent system for their ability to extract the target PAHs by soxhlet extraction method. The extracts were quantitatively analysed for sixteen PAHs using Gas Chromatography-Mass Spectrometer (GC-MS). Sonication extraction method extracted higher number of PAHs, required fewer amount of solvents, shorter time of extraction and less energy consumption compared to soxhlet extraction, which extracted higher amount of the target PAHs. The total amount of PAHs determined ranged from 0.02 mg/kg to 0.20 mg/kg in the various solvents' mixtures. The ternary mixture extracted larger quantities of the target toxic PAHs. This report will assist in the choice of analytical methods and solvent systems for environmental studies.

scholarly journals PENGARUH METODE EKSTRAKSI TERHADAP KADAR TOTAL FENOLIK, KADAR TOTAL FLAVONOID DAN AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN MENGKUDU (Morinda citrifolia L.)

2021 ◽  
Vol 18 (01) ◽  
pp. 40
Author(s):  
Septiana Laksmi Ramayani ◽  
Eka Amalia Permatasari ◽  
Indah Novitasari ◽  
Maryana Maryana
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Leaf Extract ◽  
Extraction Methods ◽  
Total Flavonoid ◽  
Morinda Citrifolia ◽  
Antihypertensive Activity ◽  
Total Phenolic ◽  
Hypertension Prevention ◽  
Assisted Extraction

ABSTRACT Excessive Reactive Oxygen Species (ROS) can cause hypertension. Prevention of the risk of hypertension is consume an antioxidant. Noni (Morinda citrifolia L). is a plant that empirically proven to lower blood pressure and has antioxidant activity. Antihypertensive activity of noni leaves is influenced by phenolic and flavonoid compounds. The extraction method affects the levels of compound in extracts. The aims of study is determine the effect of the extraction method on the total phenolic level, total flavonoid level and antioxidant activity of noni leaf extract. The extraction methods are maceration, Microwave Assisted Extraction and Soxhlet. The results showed that the different extraction methods affected the total phenol level, total flavonoid level and antioxidant activity of the noni leaf extract. The Soxlet method provides the highest total phenolic level, total flavonoid level and antioxidant activity than other extraction methods. Keywords: Noni leaf extract, extraction method, total phenolic level, total flavonoid level and antioxidant activity

scholarly journals ANTIOXIDANT, ANTI-INFLAMMATORY, AND ANTIDIABETIC ACTIVITY OF HYDROALCOHOLIC EXTRACT OF OCIMUM SANCTUM: AN IN-VITRO AND IN-SILICO STUDY

2016 ◽  
Vol 9 (5) ◽  
pp. 44 ◽  
Author(s):  
Udayabanu Malairaman ◽  
Vineet Mehta ◽  
Arun Sharma ◽  
Pallavi Kailkhura
Keyword(s):  
Plant Extract ◽  
Soxhlet Extraction ◽  
Antidiabetic Activity ◽  
In Vitro Assays ◽  
Ocimum Sanctum ◽  
Whole Plant ◽  
In Silico Study ◽  
Good Potential ◽  
Anti Inflammatory

ABSTRACTObjective: Ocimum sanctum is known to be beneficial in the management of diabetes, however, its mechanism remains unexplored. This study wasaimed to gain insite into the mechanisms through which it may counter diabetes and its complications.Methods: Hydroalcoholic whole plant extract of O. sanctum was screened for its antidiabetic potential and ability to counter oxidative andinflammatory stress through various in-vitro assays. Further, bioactive compounds that may be responsible for its antidiabetic activity were predictedthrough molecular-docking studies.Results: Crude extractive yield of 35.43% was obtained from Soxhlet extraction which mainly showed the presence of flavonoids, alkaloids, glycosides,and saponins. Plant extract showed good potential to scavenge 2,2-diphenyl-1-picrylhydrazyl free radical (40.95-68.71%) which may be attributedto its high phenolic (0.366 mg gallic acid equivalent/g) and flavonoid (0.113 mg quercetin equivalent/g) contents. Plant showed exceptional antiinflammatoryactivitywhichwasevaluatedthroughinhibition of proteindenaturation(47.61-82.37%) andredbloodcell membranestabilizationassay(43.66-78.28%).Further,extracttreatmentgreatlyinhibitedα-glucosidaseenzyme(34.17-71.45%)butfailedtoproducenoticeableinhibitionofα-amylaseactivity(1.94-14.88%).Dockingstudiespredictedthatrosmarinicacid,stigmasterol,linalool,bieugenol,andaesculinmayberesponsibleforantidiabetic activitypossessed bythe plant throughtheir interactionwith the insulin receptor.Conclusion: These findings conclude that O. sanctum may be beneficial in managing diabetes and its associated complications through inhibitingα-glucosidase activity, reducing oxidative and inflammatory stress.Keywords: Diabetes mellitus, Ocimum sanctum, Antioxidant, Anti-inflammatory, Docking, Diabetic complications.

scholarly journals The Effect of Different Fertilizer and Extraction Method on Secondary Metabolites of Azolla Pinnata

2020 ◽  
Vol 4 (1) ◽  
pp. 28-32
Author(s):  
Muhammad Ameerul Haqim Roshidi ◽  
Husna Sabrina Mahyuddin ◽  
Mohd Azrul Naim Mohamad ◽  
Abdul Latif Noh
Keyword(s):  
Secondary Metabolites ◽  
Extraction Method ◽  
Aquatic Plant ◽  
Soxhlet Extraction ◽  
Dry Weight ◽  
Extraction Methods ◽  
Chicken Manure ◽  
Similar Amount ◽  
Azolla Pinnata ◽  
Slow Moving

AbstractAzolla pinnata is an aquatic plant that comes from the order of Pteridophyta and family of Azollaceae that can be found floating on the surface of waters and can only grow at non-moving or slow-moving water areas. This research aims to understand the effect of different extraction methods and fertilizers on Azolla pinnata and its secondary metabolites. The fertilizers treatments that were tested were chicken manure and AB. The different extraction methods that were tested were SFE and Soxhlet extraction. In the test, the amount of dry weight of A.pinnata obtained from using AB fertilizer was higher compared to chicken manure. For the extraction, both SFE and Soxhlet extraction produced an almost similar amount of extracts but different amounts of secondary metabolites. The amounts of the secondary metabolites were determined and analyzed using GC-MS. The metabolites identified were theamine, bioxirane, acetic acid, 9 methyl-1-decene, glycerine, dihydroxyacetone, thymine, pyranone, 6-fluoro-2-trifluoromethylbenzoic acid, 2-formyl-4,6 dichlorophenyl ester, and isoglutamine. Based on the result, treatment with AB fertilizer provides higher amounts of secondary metabolites in A.pinnata compared to chicken manure, while for extraction methods, SFE provides higher amounts of secondary metabolites in A.pinnata compared to Soxhlet extraction.

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